Carrion Fly and Disease Vector Activity Associated with a Decomposing Corpse LTJG David England,MSC,USN LTJG David England,MSC,USN Medicolegal Entomology Medicolegal Entomology Navy Entomology Center of Navy Entomology Center of Excellence Excellence Naval Air Station Naval Air Station Jacksonville, FL Jacksonville, FL
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Carrion Fly and Disease Vector Activity Associated with a Decomposing Corpse LTJG David England,MSC,USN Medicolegal Entomology Navy Entomology Center of.
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Carrion Fly and Disease Vector Activity Associated with a Decomposing Corpse
LTJG David England,MSC,USN LTJG David England,MSC,USN Medicolegal Entomology Medicolegal Entomology
Navy Entomology Center of ExcellenceNavy Entomology Center of ExcellenceNaval Air StationNaval Air StationJacksonville, FLJacksonville, FL
ObjectivesObjectives
1.1. Basic Methods For Estimating Basic Methods For Estimating Postmortem IntervalPostmortem Interval
2.2. Oregon Decomposition And Insect Oregon Decomposition And Insect Succession ModelSuccession Model
3.3. Current Forensic Entomology Current Forensic Entomology EndeavorsEndeavors
AccumulatedAccumulated
DegreeDegree
DayDay
HoursHours
Post MortemPost Mortem
IntervalInterval
(PMI)(PMI)
Estimation of PMIEstimation of PMI Insect succession Insect succession
patternspatterns Insect life cyclesInsect life cycles Influential Influential
variablesvariablesLife Cycle
Variables
Succession
PMIPMI
Variables that influence Variables that influence PMIPMI
Succession of insects generally follows a similar Succession of insects generally follows a similar pattern at the family level, but there are pattern at the family level, but there are variations at the genus and species levels with variations at the genus and species levels with respect to differing geographical locations respect to differing geographical locations
Forensic entomology ‘works’ because succession Forensic entomology ‘works’ because succession is a very predictable and consistent eventis a very predictable and consistent event
Site LocationSite Location
Soap Creek Soap Creek Ranch,Ranch,
18km NW of 18km NW of CorvallisCorvallis
Animal ModelAnimal Model
36 kg domestic pigs36 kg domestic pigs Euthanasia methodEuthanasia method ClothedClothed CagedCaged Human homicide Human homicide
Temperature readings: ambient, carcass and Temperature readings: ambient, carcass and maggot mass maggot mass
Insect SamplingInsect Sampling
Sticky, Pitfall, Hand Sample and NetSticky, Pitfall, Hand Sample and Net One Pig Weighed Daily (Change in Mass)One Pig Weighed Daily (Change in Mass) Growth ChambersGrowth Chambers
22°C and 12 hours controlled light22°C and 12 hours controlled light Identifications - OSU Insect CollectionIdentifications - OSU Insect Collection
April, 2006April, 20066 months 6 months after cage after cage removalremoval
6 months 6 months after cage after cage removalremoval
Decomposition SummaryDecomposition Summary
Green bottle fly within 10 minGreen bottle fly within 10 min Ovipositing/egg cluster 6 hrsOvipositing/egg cluster 6 hrs 33 hrs: first hatch33 hrs: first hatch 12 hrs: predators (hornet)12 hrs: predators (hornet) 72 hrs: predators (beetles)72 hrs: predators (beetles) 168 hrs: green bottle maggots left carcass168 hrs: green bottle maggots left carcass Jan. 1Jan. 1stst little evidence left; dead vegetation little evidence left; dead vegetation