Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO

Carl Henrik Gørbitz, Ute KrengelDepartment of Chemistry, UiO

Crystal engineering

Protein crystallography

Biomarker identification

Molecules

H2

ethanesucrose

Molecules

Molecules

Crystal

Crystal

”Crystal engineering”, we make a supermolecule!

Building blocks

nodelinker

Problem 1

Molecules are very small, hard to

put together one by one

Xe-atoms on Ni-surface (Don Eigler, 1989)

Problem 2

As they are so small, we must put together very many before we reach a macroscopic size

A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules

Building blocks

Building blocks

Glue

Intermolecular forces

Must be: strong Directional

Two main types used in CE: Hydrogen bonds Metal coordination

MOFs

“Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous” (from Wikipedia)

MOF-5

Can we do this without metal ions?

Guanidinium derivatives

Fumaric acid complex

CN

N

NH

H

H

H

H

H

+

Guanidinium derivatives

DNA-based systems

DNA-based systems

DNA-based systems

Applications

Construction of polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more

Biomarker identification

Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule

Present in small amounts = hard to identify

Biomarker identification

Nano-HPLC-system

Group

Carl Henrik

Fassil

Lianglin

Malgorzata

Lise-Lotte

StevenOla

Protein crystallography

Structure/Function Studies of Structure/Function Studies of Medically Relevant SystemsMedically Relevant Systems

Department of Chemistry

Ute Krengel

University of Oslo

X-ray crystallography

Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)

MethodsMethods

Glycobiological Targets◦Mucins◦Bacterial Toxins◦Bacterial Adhesins◦Anti-tumor Antibodies◦Mushroom Lectins

Enzymes◦Chorismate mutases

GlycoNor

TargetsTargetsProtStru

ct

Glycobiological Targets:◦Bacterial Toxins – Structure and Function

Investigation of blood group dependence Delivery mechanisms? Drug design

◦Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray

structure determination

Masters ProjectsMasters Projects

GroupGroup

Ute

Øyvind

Hedda

DanielJulie

Dipankar

Dani