Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir with Potential for Hydrocarbon Biodegradation Isabel Natalia Sierra García* Microbial Resource Division -DRM Research Center for Chemistry, Biology and Agriculture – CPQBA / UNICAMP State University of Campinas – UNICAMP Campinas, SP, Brazil
27
Embed
Caracteriza§£o Estrutural e Funcional de Genes de Degrada§£o de Petr³leo Originados de
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Characterization of Metagenomic Sequences from a Brazilian Petroleum
Reservoir with Potential for Hydrocarbon Biodegradation
Isabel Natalia Sierra García*
Microbial Resource Division -DRMResearch Center for Chemistry, Biology and Agriculture – CPQBA / UNICAMP
State University of Campinas – UNICAMPCampinas, SP, Brazil
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Introduction
Methodologyand results
Conclussions
Crude oil
AlkanesSaturated hydrocarbons
Introduction
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Linear alkanesBranched alkanesCyclo alkanes
Alkanes are the major components of crude oil and the most abundant and available carbon and energy sources in reservoirs.
Microbial degradation of crude oil in reservoirs
Increase the oil viscosityAmount of sulfur
Acidity
Worldwide problem for petroleum industryrecovering and refining difficult and costly
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Pathways for aerobic and anaerobic bacterialalkane degradation
Wentzel et al. (2007). Appl Microbiol Biotechnol 76: 1209-1221
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Introduction
Several bacterial strains can degrade alkanes using enzymesystems that have still not been characterized and that mayinclude new proteins not related to those currently known
(Rojo, 2009).
Metagenomic approach
•Culture independent method which allows the access to the metabolic potential of previosly uncultured microorganisms
• In petroleum environments would allow the access to new genes or complete metabolic pathways
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Introduction
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Contig 3 1365 8 glycosyltransferase, group 2 family protein
53.9% Thiobacillusdenitrificans ATCC
25259
YP_315294.1|
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Methodology and Results
Li X, et al. Microbiol Res (2010)
Aldehyde dehydrogenases (ADLHs)
Catalyzes the oxidation of aldehydes to carboxilic acids
ADLHs are involved in the third step of the oxidation of alkanes
Alkane Alkyl alcohol Alkyl aldehyde Fatty acid
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Introduction
Methodologyand results
Conclusions
Conclusions
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Metagenomic approach have allowed the access to potentialnew sequences involved with hydrocarbon biodegradation
Complete sequencing of the shotgun library may improve theassembly and may allow a complete coverage for themetagenomic fosmid responsible for hydrocarbon degradation
Accessing new catabolic properties from such a hostileenvironment may represent an interesting source forbiotechnological use in bioremediation
For the first time in Brazil, gene sequences responsible for thedegradation processes in oil reservoirs are being identified frommicrobial metagenomic data
Characterization of Metagenomic Sequences from a Brazilian Petroleum Reservoir withPotential for Hydrocarbon Biodegradation
Conclusions
Dr. Valéria Maia de OliveiraDr. Anete Pereira de Souza