ANALYTICAL SOLUTIONS AUSTRALIA Cannabinoid Analytical and Preparative Separations Supercritical Fluid Chromatography (SFC) is a relatively new technique that offers several advantages including faster run times, better resolution and more concentrated separations. The limitations of SFC are: it has only been proven on small molecular groups and gradient development is more complicated than for HPLC. The cannabinoid group is one application that works well on SFC. The main cannabinoid species that require separation are Cannabidivarin, Cannabidiol, Cannabichromene, Tetrahydrocannabivarin, Tetrahydrocannabinol, Cannabinol, Cannabigerol, Cannabidiolic acid, Tetrahydrocannabinolic acid and Cannabigerolic acid. Column type: Eluent: Flow rate: Oven °C: Back P: Detection: Phenomenex Lux, 5μm CO and co-solvent Ethanol 5ml/min 24 100 bar DAD @ 220nm 2 ANALYTICAL SEPARATION OF CANNABINOIDS BY SFC Separation conditions: Cannabinoids Chemical Structures 1. Cannabidivarin (CBDV) 2. Cannabidiol (CBD) 3. Cannabichromene (CBC) 4. Tetrahydrocannabivarin (THCV) 5. Tetrahydrocannabinol (THC) 6. Cannabinol (CBN) 7. Cannabigerol (CBG) 8. Cannadiolic Acid (CBDA) 9. Tetrahydrocannabinolic Acid (THCA) 10. Cannabigerolic Acid (CBGA) 6 Time (min) Normalised response (220nm) 4 5 3 2 1 7 8 9 10 1. Cannabidivarin (CBDV) 3. Cannabichromene (CBC) 5. Tetrahydrocannabinol (THC) 7. Cannabigerol (CBG) 9. Tetrahydrocannabinolic acid (THCA) 2. Cannabidiol (CBD) 4. Tetrahydrocannabivarin (THCV) 6. Cannabinol (CBN) 8. Cannabidiolic acid (CBDA) 10. Cannabigerolic acid (CBGA)