Canine Adenovirus - 2 Case C08-0353 Julia Conway Resident Veterinary Anatomic Pathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC.

Canine Adenovirus - 2Canine Adenovirus - 2

Case C08-0353Case C08-0353Julia ConwayJulia Conway

ResidentResidentVeterinary Anatomic PathologyVeterinary Anatomic Pathology

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Case historyCase history 8-week old female yorkie from Miami pet

store Shipped from South America 1/5 BCS Unknown vaccination status Died with clinical signs of respiratory

infection Sample submitted

– Lung

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Morphologic DiagnosisMorphologic Diagnosis

NNecrotizing bronchiolitisecrotizing bronchiolitis

Fibrinosuppurative interstitial Fibrinosuppurative interstitial pneumoniapneumonia

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Canine AdenovirusCanine Adenovirus CAV-1CAV-1

– Infectious canine hepatitisInfectious canine hepatitis– Can cause pulmonary pathologyCan cause pulmonary pathology

CAV-2CAV-2– Primary adenoviral pneumonia rarePrimary adenoviral pneumonia rare– Associated with immunosuppressionAssociated with immunosuppression– Often in conjunction with co-infection Often in conjunction with co-infection

(CDV & CPI) or secondary infection(CDV & CPI) or secondary infection

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Canine Adenovirus 2Canine Adenovirus 2

Genus - MastadenovirusGenus - Mastadenovirus Family - AdenoviridaeFamily - Adenoviridae icosahedralicosahedral double strandeddouble stranded DNA virusDNA virus Antigenically related to CAV-1Antigenically related to CAV-1

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CAV-2CAV-2 Clinical and gross findingsClinical and gross findings

– inapparent to nonspecific respiratoryinapparent to nonspecific respiratory– bronchopneumonia and interstitial bronchopneumonia and interstitial

pneumoniapneumonia EpitheliotropicEpitheliotropic

– bronchiolar epithelium bronchiolar epithelium Bronchial epitheliumBronchial epitheliumpneumocytespneumocytes

TransmittedTransmitted– Oral, nasal and ocular secretionsOral, nasal and ocular secretionsPresented at SEVPAC 2008 – Presented at SEVPAC 2008 –

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PCR ResultsPCR Results

CAV-2CAV-2 positivepositive Canine Distemper VirusCanine Distemper Virus positivepositive Canine parainfluenzaCanine parainfluenza positivepositive

CAV-1CAV-1 negativenegative

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Morphologic DiagnosisMorphologic Diagnosis NNecrotizing bronchiolitisecrotizing bronchiolitis, subacute, , subacute,

multifocal, moderate to severe, with large, multifocal, moderate to severe, with large, basophilic, intranuclear inclusions (etiology basophilic, intranuclear inclusions (etiology consistent with consistent with AdenovirusAdenovirus) and rare eosinophilic ) and rare eosinophilic intranuclear and intracytoplasmic inclusions intranuclear and intracytoplasmic inclusions (etiology consistent with (etiology consistent with MorbillivirusMorbillivirus), lung.), lung.

Interstitial pneumoniaInterstitial pneumonia,, fibrinous and fibrinous and suppurative, subacute, diffuse, moderate with suppurative, subacute, diffuse, moderate with large, basophilic, intranuclear inclusions large, basophilic, intranuclear inclusions consistent with adenovirus, lung.consistent with adenovirus, lung.

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Acknowledgements:Acknowledgements: Jeff Abbott, DVM, PhD, Dipl. ACVPJeff Abbott, DVM, PhD, Dipl. ACVP

William Castleman, DVM, PhD, Dipl. ACVPWilliam Castleman, DVM, PhD, Dipl. ACVP

Katie Kingham, UF VMC Histology staffKatie Kingham, UF VMC Histology staff

EM – Lou Ann Miller, Center for Microscopic EM – Lou Ann Miller, Center for Microscopic imaging – UIUCimaging – UIUC

Fellow ResidentsFellow Residents

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QUESTIONS?QUESTIONS?

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