1 Research Papers of Antrodia camphorata Papers from PubMed, key word: Antrodia cinnamomea as of 4-6-2012 http://www.ncbi.nlm.nih.gov/sites/entrez Table of Contents 1: Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5 through inhibition of nuclear factor kappaB pathway . ............................................................................................................... 3 2: Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia cinnamomea using complex media. ................................................................................................................................................................ 4 3: Apoptotic effects of Antrodia cinnamomea fruiting bodies extract are mediated through calcium and calpain-dependent pathways in Hep 3B cells. ......................................................................................................................................................... 4 4: Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia cinnamomea on Propionibacterium acnes and lipopolysaccharide induced hepatic injury in mice. .................................................................................................. 4 5: Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures. ..................................................................................................................................................................................... 5 6: Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced apoptosis through the activation of adenosine A2A receptors. .................................................................................................. 5 7: Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. ..................................................................................................................................................................... 5 8: Antiangiogenic activities of polysaccharides isolated from medicinal fungi. ........................................................................ 6 9: Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. .............. 6 10: Zhankuic Acid F: A New Metabolite from a Formosan Fungus Antrodia cinnamomea. ..................................................... 7 11: New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. ...................................... 7 12: Fermented Antrodia cinnamomea Extract Protects Rat PC12 Cells from Serum Deprivation-Induced Apoptosis: The Role of the MAPK Family . ........................................................................................................................................................ 7 13: Identification and characterization of a lipase gene from Antrodia cinnamomea. ............................................................... 7 14: Maleimide and maleic anhydride derivatives from the mycelia of Antrodia cinnamomea and their nitric oxide inhibitory activities in macrophages. .......................................................................................................................................................... 8 15: Isolation and analysis of genes specifically expressed during basidiomatal development in Antrodia cinnamomea by subtractive PCR and cDNA microarray . .................................................................................................................................... 8 16. Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea. ............ 8 17. Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. ........ 9 18. Ethanolic extracts of Antrodia cinnamomea mycelia fermented at varied times and scales have differential effects on hepatoma cells and normal primary hepatocytes. ...................................................................................................................... 9 19. Cloning and heterologous expression of a novel ligninolytic peroxidase gene from poroid brown-rot fungus Antrodia cinnamomea. .............................................................................................................................................................................. 9
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Cancer Fighting Mushroom - Antrodia Cinnamomea as of 4-6-2012
Antrodia cinnamomea a.k.a. Antrodia camphorata), a precious and unique folkloric medicinal mushroom enriched in polyphenolics, isoflavonoids, triterpenoids, and polysaccharides, has been diversely used in Formosa (Taiwan) since the 18th century.
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Research Papers of Antrodia camphorata
Papers from PubMed, key word: Antrodia cinnamomea as of 4-6-2012
http://www.ncbi.nlm.nih.gov/sites/entrez
Table of Contents
1: Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5
through inhibition of nuclear factor kappaB pathway. ............................................................................................................... 3
2: Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia cinnamomea
using complex media. ................................................................................................................................................................ 4
3: Apoptotic effects of Antrodia cinnamomea fruiting bodies extract are mediated through calcium and calpain-dependent
pathways in Hep 3B cells. ......................................................................................................................................................... 4
4: Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia cinnamomea on Propionibacterium
acnes and lipopolysaccharide induced hepatic injury in mice. .................................................................................................. 4
5: Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged
6: Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced
apoptosis through the activation of adenosine A2A receptors. .................................................................................................. 5
7: Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in
8: Antiangiogenic activities of polysaccharides isolated from medicinal fungi. ........................................................................ 6
9: Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. .............. 6
10: Zhankuic Acid F: A New Metabolite from a Formosan Fungus Antrodia cinnamomea. ..................................................... 7
11: New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. ...................................... 7
12: Fermented Antrodia cinnamomea Extract Protects Rat PC12 Cells from Serum Deprivation-Induced Apoptosis: The
Role of the MAPK Family. ........................................................................................................................................................ 7
13: Identification and characterization of a lipase gene from Antrodia cinnamomea. ............................................................... 7
14: Maleimide and maleic anhydride derivatives from the mycelia of Antrodia cinnamomea and their nitric oxide inhibitory
activities in macrophages. .......................................................................................................................................................... 8
15: Isolation and analysis of genes specifically expressed during basidiomatal development in Antrodia cinnamomea by
subtractive PCR and cDNA microarray. .................................................................................................................................... 8
16. Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea. ............ 8
17. Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. ........ 9
18. Ethanolic extracts of Antrodia cinnamomea mycelia fermented at varied times and scales have differential effects on
hepatoma cells and normal primary hepatocytes. ...................................................................................................................... 9
19. Cloning and heterologous expression of a novel ligninolytic peroxidase gene from poroid brown-rot fungus Antrodia
33. Anti-inflammatory effects of methanol extract of Antrodia cinnamomea mycelia both in vitro and in vivo..................... 14
34. 4-Acetylantroquinonol B Isolated from Antrodia cinnamomea Arrests Proliferation of Human Hepatocellular Carcinoma
HepG2 Cell by Affecting p53, p21 and p27 Levels. ................................................................................................................ 15
35. Developmental Toxicity Assessment of Medicinal Mushroom Antrodia cinnamomea T.T. Chang et W.N. Chou (Higher
Basidiomycetes) Submerged Culture Mycelium in Rats. ........................................................................................................ 15
36. Cytochrome P450 Genes in Medicinal Mushroom Antrodia cinnamomea T.T. Chang et W.N. Chou (Higher
Basidiomycetes) are Strongly Expressed During Fruiting Body Formation. ........................................................................... 16
37. Methanol extract of Antrodia cinnamomea mycelia induces phenotypic and functional differentiation of HL60 into
monocyte-like cells via an ERK/CEBP-β signaling pathway. ................................................................................................. 16
38. Proteomic analysis of differently cultured endemic medicinal mushroom antrodia cinnamomea T.T. Chang et W.N. Chou
from Taiwan. ............................................................................................................................................................................ 16
39. Medium modification to enhance the formation of bioactive metabolites in shake flask cultures of Antrodia cinnamomea
by adding citrus peel extract. ................................................................................................................................................... 17
40. Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells
Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways. ........... 17
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41. Ethanol extracts of fruiting bodies of Antrodia cinnamomea exhibit anti-migration action in human adenocarcinoma
CL1-0 cells through the MAPK and PI3K/AKT signaling pathways. ..................................................................................... 18
Below are from ScienceDirect ................................................................................................................................................. 19
1: Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture ..................... 19
2: The hepatoprotective activity against ethanol-induced cytotoxicity by aqueous extract of Antrodia cinnamomea ............. 19
3: Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in
submerged culture of Antrodia cinnamomea ........................................................................................................................... 19
4: Characterization and biological functions of sulfated polysaccharides from sulfated-salt treatment of Antrodia
5: The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures ....... 20
6: Triterpenoids from Antrodia cinnamomea ........................................................................................................................... 21
7: Purification and partial characterization of a lipase from Antrodia cinnamomea ................................................................ 21
8: A sesquiterpene lactone, phenyl and biphenyl compounds from Antrodia cinnamomea ..................................................... 21
9: Glucose stimulates production of the alkaline-thermostable lipase of the edible Basidiomycete Antrodia cinnamomea ... 21
10: Antrodia cinnamomea sp. nov. on Cinnamomum kanehirai in Taiwan .............................................................................. 22
11. Steroids and triterpenoids of Antodia cinnamomea—A fungus parasitic on Cinnamomum micranthum .......................... 22
12. Characterization and functional elucidation of a fucosylated 1,6-α-D-mannogalactan polysaccharide
from Antrodiacinnamomea ...................................................................................................................................................... 22
13. Chemical profiling of the cytotoxic triterpenoid-concentrating fraction and characterization of ergostane stereo-isomer
ingredients from Antrodia camphorata ................................................................................................................................... 22
14. Analysis of volatile compounds of Antrodia camphorata in submerged culture using headspace solid-phase
15. Development of a LC–MS/MS method for the determination of antrodin B and antrodin C from
Antrodia camphorata extract in rat plasma for pharmacokinetic study ................................................................................... 23
1: Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line
PLC/PRF/5 through inhibition of nuclear factor kappaB pathway.
Food Chem Toxicol. 2007 Jul;45(7):1249-57. Epub 2007 Jan 17.
Hsu YL, Kuo PL, Cho CY, Ni WC, Tzeng TF, Ng LT, Kuo YH, Lin CC.
Department of Pharmacy, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
In this study, we first report the anti-invasive effect of ethylacetate extract from Antrodia cinnamomea (EAC) fruiting
bodies in the human liver cancer cell line PLC/PRF/5. Treatment with EAC decreased the cancer invasion of PLC/PRF/5
cells in a dose-dependent manner. This effect was strongly associated with a concomitant decrease in either the level or
activity of VEGF, MMP-2, MMP-9 and MT1-MMP, and an increase in the expression of TIMP-1 and TIMP-2. EAC
inhibited constitutively activated and inducible NF-kappaB in both its DNA-binding activity and transcriptional activity.
Furthermore, EAC also inhibited the TNF-alpha-activated NF-kappaB-dependent reporter gene expression of MMP-9 and
VEGF, and the invasion of cancer cells. EAC also exhibited an inhibitory effect on angiogenesis in a Matrigel Plug
Angiogenesis Assay. Further investigation revealed that EAC's inhibition of cancer cell growth and invasion was also
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evident in a nude mice model. Our results indicate that EAC inhibits the activation of NF-kappaB, and may provide a
molecular basis for drug development using EAC as an anti-invasive agent in the prevention and treatment of cancer.
PMID: 17316946 [PubMed - in process]
2: Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia
cinnamomea using complex media.
Bioresour Technol. 2007 Sep;98(13):2511-7. Epub 2006 Oct 27.
Lin ES, Chen YH.
Department of Cosmetic Science, Vanung University, No. 1 Van-Nung Road, Chung-Li, Taoyuan 320, Taiwan, ROC.
Submerged cultures were used to identify growth-limiting nutrients by Antrodia cinnamomea strains. The mycelial biomass
and EPS production by A. cinnamomea BCRC 35396 were markedly higher than other A. cinnamomea strains. A relatively
high C/N ratio was favorable for both the mycelial growth (5.41g/l) and EPS production (0.55g/l); the optimum ratio was 40.
The glucose was available utilized preferentially for mycelial growth, rather than for EPS production. Flushing the culture
medium with nitrogen had a stimulating effect on both mycelial growth and EPS production. In addition, peptone, yeast
extract and malt extract appeared to be important and significant component for EPS production. Phosphate ion, magnesium
ion and thiamine were probably not essential for mycelial growth. By optimizing the effects of additional nutrition, the
Ming-Chi Tsai, Tuzz-Ying Song, Ping-Hsiao Shih and Gow-Chin Yen aDepartment of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227,
Taiwan bDepartment of Nutrition and Health Science, Chungchou Institute of Technology, 6, Lane 2, Sec 3, Shan-chiao Road,
Yuanlin, Changhwa 51003, Taiwan
Received 27 September 2006; revised 10 January 2007; accepted 10 January 2007. Available online 25 January 2007.
Abstract: Antrodia cinnamomea, a well-known tradition Chinese medicine, possesses anti-tumor, anti-oxidation activities
and stimulates the immune system. The aim of this study was to investigate the protective effect of water-soluble
polysaccharides from the fermented filtrate and mycelia of Antrodia cinnamomea in submerged culture (ACSC) on
hydrogen peroxide-induced cytotoxicity and DNA damage in Chang liver cells. Oxidative DNA damage was evaluated by
single cell gel electrophoresis (Comet assay) or by the formation of 8-hydroxy-deoxyguanosine (8-OHdG) adducts. The
polysaccharides isolated by ion-exchange chromatography contained glucose, xylose, galactose, arabinose, and mannose.
The results showed that incubation of Chang liver cells with isolated polysaccharides at 200 µg/mL for 5 h prior to H2O2
treatment (50 µM, 30 min) significantly reduced oxidative DNA damage as detected by the formation of comet tail DNA
and 8-OhdG adducts by 89% and 69%, respectively. Pre-treatment Chang liver cells with polysaccharides also reduced the
levels of thiobarbituric acid reactive substances (TBARS) (p < 0.01) and intracellular reactive species (ROS) (p < 0.01)
induced by H2O2. Moreover, glutathione S-transferase (GST) and the GSH/GSSG ratio were significantly increased in
Chang liver cells pre-incubated with the polysaccharides (p < 0.01). These results demonstrate that polysaccharides in
ASCS have antioxidant properties which may involve up-regulation of GST activity, maintenance of normal GSH/GSSG
T.T. Chang1 and W.N. Chou2 1Division of Forest Protection, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, Taiwan 2National Museum of Natural Science, Taichung, Taiwan
A new basidiomycete Antrodia cinnamomea sp. nov., causing brown heart rot of Cinnamomum kanehirai in Taiwan, is
described and illustrated.
11. Steroids and triterpenoids of Antodia cinnamomea—A fungus parasitic on Cinnamomum micranthum
Phytochemistry, Volume 41, Issue 5, March 1996, Pages 1389-1392, doi:10.1016/0031-9422(95)00767-9
Shu-Wei Yang*, Ya-Ching Shen†, and Chung-Hsiung Chen*, * School of Pharmacy, National Taiwan University, Taipei, Taiwan, Republic of China † Institute of Marine Resources, National Sun Yat-sen University, 70 Lien-Hai Rd, Kaohsiung, Taiwan, Republic of China
Available online 10 September 1998.
Abstract: Two ergostane related steroids, zhankuic acids D and E together with three lanosta related triterpenes,
15α-acetyl-dehydrosulphurenic acid, dehydroeburicoic acid, dehydrosulphurenic acid were isolated from the fruit body of
the fungus Antrodia cinnamomea. Their structures were determined by spectral analyses and comparison with known
compounds.
12. Characterization and functional elucidation of a fucosylated 1,6-α-D-mannogalactan polysaccharide
Ming-Hong Yena, Ya-Ting Tsuia, Hsuan-Lun Chena, Ming-Feng Houc, Mei-Chin Lua, f, g, 2, , Yang-Chang Wua, h, i, , a Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan b Research and Development Center of Chinese Herbal Medicines and New Drugs, College of Pharmacy, Kaohsiung Medical
University, Kaohsiung 807, Taiwan c Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan d Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan e Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
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f Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan g National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan h Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan i Natural Medicinal Products Research Center, and Center for Molecular Medicine, China Medical University Hospital,
Taichung 404, Taiwan
Received 28 June 2011; revised 9 September 2011; Accepted 12 September 2011. Available online 16 September 2011.
Abstract: Antrodia camphorata (AC), also known as A. cinnamomea, an endemic species in Taiwan, is one of the treasured
medicinal mushrooms. AC is traditionally used for its chemopreventive biofunctions. In this investigation, we report a
convenient method for concentrating the antiproliferative active triterpenoid-rich fraction (FEA), from ethanolic extract of
AC (EEAC). A series of stereo-isomers of zhankuic acids (1-8) from the FEA was purified by HPLC using an efficient
acidic solvent system. The structures of compounds 1-8 were elucidated based on spectroscopic data analysis, and the
absolute configuration of α-chiral carboxylic acid at C-25 in the structures was assigned based on reaction with (R)- and
(S)-1-(9-anthryl)-2,2,2-trifluoroethanol. Major ingredients of FEA (eight ergostanes 1-8 and two lanostanes 9-10) were
further characterised by high-performance liquid chromatography-photodiode array detection/mass spectrometry
(HPLC-PDA/MS). Compounds 1-8 and their pair mixture forms (antcin K, antcin C, zhankuic acid C, and zhankuic acid A)
were subjected to anti-proliferative assay against three human leukemia cell lines. Among them, the derivatives with
carbonyl group at C-3 showed cytotoxicity with IC50 values ranging from 16.44 to 77.04 µg/ml.
14. Analysis of volatile compounds of Antrodia camphorata in submerged culture using headspace solid-phase
a Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122,
PR China b The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China c State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China
Received 22 June 2009; revised 29 October 2010; Accepted 29 December 2010. Available online 8 January 2011.
Abstract: In this work a headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass
spectrometry (GC–MS) and GC–olfactometry (GC–O) was developed to evaluate the profile of the volatile compounds that
contribute to the aroma of Antrodia camphorata in submerged culture. For this purpose, the HS-SPME sampling method for
the volatile compounds of A. camphorata in submerged culture was optimised by a D-optimal design. A HS extraction of
the culture broth of A. camphorata followed by incubation on a carboxen/polydimethylsiloxane (CAR/PDMS) fibre during
31.8 min at 54.6 °C gave the most effective and accurate extraction of the volatile compounds. By the optimised method, a
total of 49 volatile compounds were identified in culture broth of A. camphorata, while a total of 55 volatile compounds
were identified in the mycelia. A series of C8aliphatic compounds (mushroom-like odour), several lactones (fruity odour)
and L-linalool (citrus-like odour) were the most potent key odourant in both the mycelia and culture broth. This combined
technique is fast, simple, sensitive, inexpensive and useful to monitor volatile compounds associated to A. camphorata.
Research highlights
► Volatiles of fermented Antrodia camphorata were analysed by HS-SPME–GC–MS. ► Aroma of fermented A.
camphorata was evaluated by HS-SPME–GC–O. ► HS-SPME condition for the volatiles was extensively optimised by a
D-optimal design.
15. Development of a LC–MS/MS method for the determination of antrodin B and antrodin C from
Antrodia camphorata extract in rat plasma for pharmacokinetic study
Yongli Liua, b
, Xin Dia, Xingchao Liu
a, Wenjin Shen
a, Kelvin Sze-Yin Leung
b a School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China b Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong, PR China
Received 18 March 2010; revised 14 May 2010; Accepted 15 May 2010. Available online 12 June 2010.
Abstract: A selective and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was
developed and validated for the determination of antrodin B and antrodin C in rat plasma. Both target compounds, together
with the internal standard (diazepam), were extracted from rat plasma samples by liquid–liquid extraction with ethyl acetate.
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Chromatographic separation was carried out on an Agilent XDB-C8 column with an isocratic mobile phase consisting of
acetonitrile and water (70:30,V/V) at a flow rate of 0.5 mL/min. The mass spectrometric detection was performed by
selected reaction monitoring (SRM) mode via atmospheric pressure chemical ionization (APCI) source operating in positive
ionization mode. The assay exhibited a linear dynamic range of 47.6–4760 ng/mL for antrodin B and 56.6–5660 ng/mL for
antrodin C. The intra- and inter-day precision was less than 5.3% and the accuracy was less than 2.7% for both analytes.
The validated method has been applied to the pharmacokinetic study of antrodin B and antrodin C in rats following oral
administration of Antrodia camphorata extract.
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