Camera Excitation Filter Wheel Emission Filter Wheel Automated Interchangeable Dichroic Mirror Splitter Microscopy Body Lens Xenon Arc Lamp Sutter Controller Computer MetaFluor Image Acquisition Image Analysis Linear Polarized Fiber Laser Beam Steerer Piezoelec tric Mirror Laser Beam Fluorescence Neutral Density
Camera Excitation Filter Wheel Emission Filter Wheel Automated Interchangeable Dichroic Mirror Splitter Microscopy Body Lens Xenon Arc Lamp Sutter Controller.
Dec 30, 2015
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Camera
Excitation Filter Wheel
Emission Filter Wheel
Automated InterchangeableDichroic Mirror
Splitter
Microscopy Body
Lens
Xenon Arc Lamp
Sutter Controller
Computer
MetaFluorImage Acquisition
Image Analysis
Linear Polarized Fiber Laser
Beam Steerer
Piezoelectric Mirror
Laser Beam
Fluorescence
Neutral Density
Excitation Filter (nm)
Dichroic Mirror (long pass; nm)
Emission Filter (nm)
CFP420/20 450
475/40
YFP (FRET) 535/25
mOrange2548/20 560
572/20
mCherry (FRET) 630/20
mCherry 580/20 600 630/20
Table 1. The settings of filters for the Multicolor FRET imaging.
Camera
GFP/RFPNikon
Eye
A B
A/B
CFP FRET
Ratio Pseudo Color
Color
BrightnessIMD Display Color
Brightness
IMD Display Color
Brightness
1.6
0.950.95
1.15
1.35
1.55
-8 -4 0 4 8 12 16
Lyn
Kras
Ratio<0.95
Ratio>1.6
1.6
0.95
1.35
0.95
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