Calling onScience - AIM Group€¦ · Cornelis Brekelmans, Belgium Marilyn Brown, USA Donald Broom, UK JoseV. Castell, Spain Isabella De Angelis, Italy Sebastien Farnaud, UK Suzanne

Rome (Italy)August 30 - September 3, 2009

CallingonScienceCallingonScience

VI I Wor ld Congresson Al ternat ives

& Anima l Use in theL i fe Sc iences

VI I Wor ld Congresson Al ternat ives

& Anima l Use in theL i fe Sc iences

Final programme

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Index of contentsIndex of contentsWelcomeCongress CommitteesProgramme overviewAcknowledgementsAwards and grantsScientific and practical informationGeneral informationAbout RomeExhibition and Congress floorplansRegistrationsSocial programmePre-Congress Satellite MeetingsScientific programme:- Monday, August 31, page 25- Tuesday, September 1, page 31- Wednesday, September 2, page 37- Thursday, September 3, page 43Poster exhibitionIndex of chairpersons, speakers and presenting authorsIndex of sessionsIndex of posters

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CoCoWelcomeWelcome

Dear WC7 participants,Since we took the challenge, in an euphoric moment during WC5 in Berlin, to organizethe 7th World Conference on Animal Use and its Alternatives, in short WC7, a lot haschanged both in the world of alternative methods and in our private lives. Both of uschanged jobs and moved our families away from beautiful Italy:Thomas took the posi-tion of Director of CAAT and Professor of Evidence-basedToxicology at the Johns Hop-kins University in Baltimore, USA and Herman went to Brussels to head a newnon-profit scientific organization (Orange House Partnership) with the aim to providescientific assistance in the area of chemical and food safety to developing countries.More importantly, however, we have seen since the start of REACH and the animal te-sting ban for cosmetic ingredients in Europe, the milestone publication of the NationalResearch Council for toxicity testing in the 21st century in the US and the creation ofJaCVAM in Japan to mention only a few highlights.We have had the successful WC6 inTokyo and we have experienced that the field of the 3R’s has expanded with more andmore methods available, validated and accepted.We have witnessed that globalizationis at work, that our wish to see more formal international collaboration has come true.We are aware that what was until recently termed “alternative” has become mainstreamscience and an integral part of life sciences.These developments have created businessopportunities and can solve regulatory problems, most notably in priority setting. Alltogether the field is moving to the forefront of scientific developments, leading the pathfor quality assurance and predictive usefulness.Thus, it seemed timely to give WC7 the motto “CALLING ON SCIENCE”, both in thesense of challenging established sciences with new approaches and in calling for thesound scientific base for innovative technologies and concepts. A substantially increa-sed number of sponsors, a record submission of abstracts and the support of many in-dividuals and friends have made it possible to welcome you in this beautiful part ofRome.We strongly believe that we can offer you an outstanding opportunity to meetwith colleagues and friends, share and discuss progress and return inspired to your of-fices and laboratories at home. On the occasion of the 50th birthday of Russell & Bur-ch’s renowned 3R principles you will experience a flourishing field ready for thechallenges of the life sciences in the 21st century.We hope you will enjoy WC7 as much as we intend to do.

Herman B.W.M. Koëter Thomas Hartung

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Congress CommitteesCongress Committees

Patric Amcoff, FranceSara Amundson, USANirmala Bhogal, UKBas J. Blaauboer,The NetherlandsCornelis Brekelmans, BelgiumMarilyn Brown, USADonald Broom, UKJose V. Castell, SpainIsabella De Angelis, ItalySebastien Farnaud, UKSuzanne Fitzpatrick, USAFranz Paul Gruber, Switzerland

Barbara Grune, GermanyTohru Inoue, JapanAbigail Jacobs, USARobert Kavlock, USATsutomu Miki Kurosawa, JapanMark Lafranconi, USAManfred Liebsch, GermanyEmily McIvor, UKDavid J. Mellor, New ZealandFrauke Ohl,The NetherlandsPaul W.J. Peters,The NetherlandsChristopher J. Portier, USA

Octavio Presgrave, BrazilChristoph A. Reinhardt, SwitzerlandAmy Rispin, USAVera Rogiers, BelgiumBart Sangster,The NetherlandsLeonard Schechtman, USAMichael Schwarz, GermanyHasso Seibert, GermanyTroy Seidle, CanadaEllen Silbergeld, USANoriho Tanaka, JapanMassimo Tettamanti, Italy

Sonja Van Tichelen, BelgiumBert Van Zutphen,The NetherlandsGilman Veith, USASimon Webb, BelgiumCarlWestmoreland, UKNeil L.Wilcox, USACatherine Willet, USAMarilyn Wind, USAMaria Flavia Zucco, Italy

Ivan Arisi, ItalyEmilio Benfenati, ItalyCornelis Brekelmans, BelgiumChristof J. Burek, GermanyNeil G. Carmichael, BelgiumCecilia Clemedson, SwedenRaffaella Corvi, ItalyRodger Curren, USAOdile de Silva, FranceRoland Dobbelaer, BelgiumJeffrey Everitt, USAElaine M. Faustman, USASuzanne C. Fitzpatrick, USA

Alan M. Goldberg, USABarbara Grune, GermanyGerhard Gstraunthaler,AustriaMarlies Halder, ItalyMakoto Hayashi, JapanAndreas Hess, GermanySebastian Hoffmann, GermanyAbigail Jacobs, USAJoanna Jaworska, BelgiumRobert Kavlock, USAJos Kleinjans,The NetherlandsHolger Knaut, USAHajime Kojima, Japan

Roman Kolar, GermanyJoachim Kreysa, ItalyHarry A. Kuiper, GermanyMiki Tsutomu Kurosawa, JapanMario L. Chovel Cuervo, CubaPamela J. Lein, USAPauline McNamee, UKSiegfried Morath, USADavid Morton, FranceChristine Mummery,The NetherlandsWalter Pfaller, AustriaErwin L. Roggen, DenmarkAgapios Sachinidis, Germany

Leonard Schechtman, USAKristine Schirmer, SwitzerlandGerrit Schüürmann, GermanyMichael Schwarz, GermanyWilliam S. Stokes, USAVicki Stone, UKRaymond Tice, USAJan van der Valk,The NetherlandsPhilippe Vanparys, BelgiumJoanne Zurlo, USAMarilyn L.Wind, USA

Congress Co-ChairsThomas Hartung, USA

Herman B.W.M. Koëter, Belgium

Steering CommitteeUmberto Agrimi, Italy

Clément Gauthier, CanadaCoenraad Hendriksen,The Netherlands

Marcel Leist, GermanyMartin Stephens, USA

Alternatives Congress TrustMichael Balls, UK

Alan Goldberg, USACoenraad Hendriksen,The Netherlands

Andrew N. Rowan, USAHorst Spielmann, Germany

Scientific Organizing CommitteeThe Congress Co-Chairs gratefully acknowledge the following Session Chairs for

having actively contributed to the organization of the scientific sessions:

Programme Review PanelThe Congress Co-Chairs gratefully acknowledge the following experts for their help with

the Congress scientific programme:

AIM Group – AIM CongressMilan OfficeVia G. Ripamonti, 12920141 Milano, Italye-mail:[email protected]

RegistrationsAIM Group – AIM CongressRome OfficeVia Flaminia, 106800189 Roma, Italye-mail:[email protected]

Hotel ReservationAIM Group – AIM DMCMilan OfficeVia G. Ripamonti, 12920141 Milano, Italye-mail:[email protected]

Scientific and Organizing Secretariat

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Programme overviewProgramme overview

Auditorium Room Cavalieri 1 Room Cavalieri 2 Room Ellisse Terrazza Monte Mario Room San PietroHrs

Welcome Cocktail and Get-Together Party (Gardens of the Cavalieri Hotel)

Registrations (Garden Lobby) and posters set up

SUNDAY AUGUST 30PRE-SATELLITE MEETINGS AND CONGRESS REGISTRATIONS

SYMPOSIUMco-organized by

CELLTOX and ESTIV"From tissueengineering to

alternatives: research,discovery anddevelopment"

JOINT MEETING ofAltweb Project Teamand 3Rs Centers

organized by the JohnsHopkins Center for

Alternatives to AnimalTesting (CAAT)

ANIMAL PROTECTIONSATELLITE MEETING"Bringing togetherInternational Animal

ProtectionOrganisations"

organized by HumaneSociety of the UnitedStates (HSUS) and

Eurogroup for Animals

Auditorium Room Cavalieri 1 Room Cavalieri 2 Room Ellisse Terrazza Monte Mario Room San PietroHrs

08:00-09:00

09:05-09:15

09:15-10:00

10:10-12:40

12:40-13:40

13:40-14:40

14:50-17:20

17:30-18:15

20:30-23:00

Lunch - poster exhibition visit

Garden Party (Gardens of the Cavalieri Hotel)

MONDAY AUGUST 31THEME 1: INNOVATIVE TECHNOLOGIES, CONCEPTS AND APPROACHES

Early Morning SessionAnimal WelfareAssociations

Plenary session 1The U.S. Tox21Community and thefuture of toxicologytesting

Opening ceremony

Early Morning SessionIndustry activities 1

Early Morning SessionAsian regulatoryaffairs in Animal Welfareand alternatives

Breakout sessionIn vitro technologies

Breakout sessionOmics and systemsbiology

Plenary session 2Animal health andwelfare in the foodchain: food for thought

Breakout sessionNon-invasivetechnologiesBreakout sessionNon-vertebrate models Breakout sessionDatabases: scientificapproaches

Breakout sessionCurrent and evolvingconcepts for thevalidation of safetyassessment method

Breakout sessionIntegrated approaches Breakout sessionHigh throughputtechnologiesBreakout sessionIn silico models Breakout sessionChemical and physicalmethods

10:10-11:10 Extrabreakout sessionStatus report onPREDICT-IV11:30-12:30 Extrabreakout sessionStatus report onCAESARLunch sessionDatabases: progressreportLunch sessionGood Cell CulturePractices

14:00-15:00

15:00-16:00

16:00-17:00

17:00-18:00

18:00-19:00

19:00-20:00

20:00-22:00

14:50-15:50 Extrabreakout sessionStatus report onEvidence BasedToxicology Project16:00-17:00 Extrabreakout sessionUpdate on ICH andVICH Progress

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Early morning sessionAssociations

Breakout sessionChemicals & pesticidesBreakout sessionBasic research

Breakout sessionCosmetics

Plenary session 4Metabonomics-driventop-down systemsbiology: techniquesand applications

Breakout sessionVaccines andbiologicalsBreakout sessionFood improving agents Breakout sessionNanomaterial toxicitytesting

Breakout sessionAnimal use policies

14:45-15:45 Extrabreakout sessionStatus report onReProTect16:00-17:00 Extrabreakout sessionStatus report onOSIRIS

Breakout sessionPharmaceuticalsBreakout sessionGenetically modifiedorganisms

Breakout sessionEducationand tranining

Lunch session2009 developments inthe field of alternativemethodsLunch sessionRecent progress andfuture directions inthe validation andregulatory acceptanceofalternative testmethods that reduce,refine and replaceanimal use

10:00-11:00 Extrabreakout sessionStatus report onICATM11:10-12:10 Extrabreakout sessionStatus report on EPAA

Early morning session3R centers 2

Plenary session 3Brueghel's two

monkeys: passing thefinal exam in the history

of mankind

Early morning sessionEducational activities

Auditorium Room Cavalieri 1 Room Cavalieri 2 Room Ellisse Terrazza Monte Mario Room San PietroHrs

08:00-09:00

09:10-09:55

10:00-12:30

12:30-13:30

13:30-14:30

14:45-17:15

17:25-18:10

TUESDAY SEPTEMBER 1THEME 2: AREAS OF ANIMAL USE

Lunch - poster exhibition visit

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Calling for Science - Answering the CallThe European Commission launched a 25 Million € call for proposals towards a strategy towards

alternatives to repeat-dose systemic safety tests using animals.The cosmetic industry has pledged toprovide financial support to projects selected by the European Commission with the same amount.

Two information events around this call will be held at the occasion of the WC7:Monday, August 31: General Introduction to the Research Project

18:30-20:00 - Room EllisseTuesday, September 1: Bringing together interested scientists

18:30-20:00 - Room San PietroParticipation is open. No attendance fee is required.

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Auditorium Room Cavalieri 1 Room Cavalieri 2 Room Ellisse Terrazza Monte Mario Room San PietroHrs

08:00-09:00

09:10-09:55

10:00-12:30

12:30-13:30

13:30-14:30

14:45-17:15

17:25-18:10

20:00-23:00

WEDNESDAY SEPTEMBER 2THEME 3: PROGRESS IN LIFE SCIENCE DOMAINS

Plenary session 5Predictive testingstrategies: R&Dachievements andperspectives

Breakout sessionSkin and eye toxicity I

Breakout sessionSkin and eye toxicity II Breakout sessionGenotoxicity andcarcinogenicityBreakout sessionEnvironmental science Breakout sessionAnimal welfare science Breakout sessionNeuroscience

14:45-15:45 Extrabreakout sessionStatus reporton Sens-itiv16:00-17:00 Extrabreakoutsession Status reporton ESNATS

Breakout sessionSystemic toxicity andtarget organsBreakout sessionReproduction,development andfertility

Breakout sessionDisease models Breakout sessionImmunology

10:00-11:00 Extrabreakout sessionStatus report onACuteTox11:10-12:10 Extrabreakout sessionStatus report onToxCastLunch sessionAnimal use policies:the future of AnimalWelfare LegislationLunch sessionVaccines:acceptance of 3Rmethods

Early morning sessionIndustry activities 2 Early morning session3R Centres 3

Lunch - poster exhibition visit

Early morning sessionLessons learnedfrom the validationand potentialregulatoryapplicability ofin vitro alternativepyrogen tests

Plenary session 6Is forefront scienceconsidered and appliedin regulatory riskassessment?

Congress Dinner (Villa Miani)

Programme overviewProgramme overview AA

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Plenary session 7- The principles of humane experimental technique:timeless insights and unheeded warnings- The principles of humane experimental technique:is it relevant today?

Awards ceremony

Special recognition event

Plenary session 8Calling on science: making alternatives the newtoxicity-testing gold standard

WC8 presentation

Closing speeches

AuditoriumHrs

THURSDAY SEPTEMBER 3SPECIAL EVENT: 50 YEARS AFTER RUSSEL & BURCH

09:00-09:45

09:45-10:00

10:45-11:15

11:15-12:00

12:00-12:15

12:15-12:45

WC7 side meetingsGeneral Assembly of EUSAAT

Monday, August 3118:30-19:30 - Room San Pietro

Project Board Meeting CarcinoGENOMICSDevelopment of a high throughput genomics-based

test for assessing genotoxic and carcinogenic propertiesof chemical compounds in vitro

Tuesday, September 118:15-22:00 - Room Terrazza Monte Mario

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The Congress Co-Chairs and the Alternatives Congress Trust would like to express their gratitude toall those who have generously supported the Congress:

PLATINUM SPONSORS AND PATRONS OF THE WC7

GOLD SPONSORS AND BENEFACTORS6th World Congress, Japan

SILVER SPONSORS

BRONZE SPONSORS

CONGRESS SUPPORTERSAGAATI Foundation,The Netherlands

CCAC (Canadian Council on Animal Care), CanadaCognis, Germany

GlaxoSmithKline, UKIntervet/Schering-Plough Animal Health,The Netherlands

NIEHS, USANotox,The NetherlandsNovozymes, Denmark

SOT (Society of Toxicology), USATNO,The Netherlands

AcknowledgementsAcknowledgements

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Awards and grantsAwards and grantsThe Humane Society of the United States’ 2009 Russell & Burch AwardThe HSUS presents the Russell and Burch Award to scientists who have made outstanding contributions to the advance-ment of alternative methods in the areas of biomedical research, testing, or higher education.The award, named in honorof William Russell and Rex Burch, who formulated the Three Rs approach, carries a $ 5,000 prize and is a means of reco-gnizing the important role that scientists play in limiting the use and suffering of animals in laboratories. Candidates arejudged on the scientific merit of their contribution to the alternatives field, as well as on their impact and professional com-mitment to this field.Charles River Laboratories’ Excellence in Refinement AwardThe Charles River Laboratories’ Excellence in Refinement Award, sponsored by Charles River’s Commitment to HumaneAnimal ResearchThrough Excellence and Responsibility (CHARTER) Program (formerly known as the Charles River Foun-dation), in cooperation with the Johns Hopkins Center for Alternatives to AnimalTesting (CAAT), honors an individual whohas made an outstanding contribution to the development, promotion and/or implementation of refinement alternatives.This award is based on the conviction that the humane care of laboratory animals is both a moral imperative and a scien-tific necessity. The award, which includes $ 5,000 to further the recipient’s scientific endeavors, is presented during theWorld Congress on Alternatives and Animal Use in the Life Sciences.CAAT’s Recognition AwardThis award, presented at every World Congress, honors an individual or organization who has made an outstanding con-tribution to the field of the 3Rs, the development of alternative methods, or the field of in vitro science.Doerenkamp-Zbinden Foundation AwardsThe origins of the DZ Foundation (DZF) lie in the award of a yearly prize for distinguished services to animal protec-tion in science. Next to scientists, persons in the fields of animal protection or philosophy and public persons may beawarded the DZ-Prize in so far as they have distinguished themselves in the sense of the replacement and reductionof animal experiments.The prize 2009 is bestowed with CHF 25,000 and can be divided.Laureates of the DZF:2009 Cross-agency workgroup that produced The U.S. Environmental Protection Agency’s

Strategic Plan for Evaluating the Toxicity of Chemicals (U.S. EPA 2009)2007 Günter Verheugen,Vice President of the European Commission (EU)2007 zet (Austria) and Animal Welfare Academy (Germany)2006 David Dewhurst (UK)2005 Herman B.W. M. Koëter (Italy) and Manfred Liebsch (Germany)2004 Franz P. Gruber (Switzerland)2002 Coenraad Hendriksen (The Netherlands)2001 Alan Goldberg and Ian Kimber (USA)2000 Vera Rogier (Belgium) and Horst Spielmann (Germany)1999 Andrew Rowan (USA)1998 Bert van Zutphen (The Netherlands)1997 Michael Irwin, R. Moffatt and Carl Pinkert (USA)1996 Thomas Hartung and Albrecht Wendel (Germany)1990 Paul Flecknell (UK)1989 Beat Gaehwiler (Switzerland) and Harvey S. Singer (USA)1988 Charles E. Branch (USA)1987 James E. Heavner (USA)ROME 2009, DZ Poster AwardsTo sponsor the World Congress on Alternatives 2009 in Rome the DZ Foundation board decided to post 15 awards forthe best posters in the sense of the principles of the DZF.The members of the FB and the SAB present at WC7 will decide to whom this awards shall be given. Direct applicationsfor the awards are not possible.The awards are bestowed with € 1,000.00 each.BHMF AwardThe Bo Holmstedt Memorial Fund (BHMF) donates twice a year an award of € 1,000.00 for reasearchers who presenton their posters a promising way to solve a toxicological problem by a technique that respects the 3 R-principle.

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Dieter Lütticken AwardIntervet/Schering-Plough Animal Health, a leading global animal health company, sponsors the international Dieter Lüttic-ken Award for alternatives in animal testing to support individual scientists and life science research institutions that makesignificant contributions to the 3R-concept, i.e. reducing, refining and/or replacing the use of animals in testing for deve-lopment and manufacturing of veterinary medicines.The total amount of the award is € 20,000.00 and will be presentedduring the award session. Applications for the Dieter Lütticken Award 2009 can be submitted until November 15, 2009.For submissions and more details, please contact Intervet/Schering-Plough Animal Health - Global Communications Ani-mal Health ([email protected]).Dr. Ethel Thurston ScholarshipsThe American Fund for Alternatives to Animal Research (AFAAR) has awarded four scholarships of $ 2,500 to doctoral stu-dents of merit who demonstrate an interest and priority in replacing the use of animals in research and teaching and wi-shed to take part in the WC7.The awards are made under the direction of the New England Anti-Vivisection Society incollaboration with the British Union for the Abolition of Vivisection to honor AFAAR's founder, the late Dr. Ethel Thur-ston. The candidates who have been selected are:Sara Aghazadeh, IranSuzan Commandeur,The NetherlandsCristiana Nicoli De Mattos, BrazilLavinia Stingl, Germany

WC7 Participation GrantsThe WC7 has provided eleven Participation Grants to those participants with limited financial resources, whose abstractshave been selected for either oral or poster presentation. Participation grants cover the registration fee and offer a lump-sum refund of travel and accommodation expenses, according to the Country of origin of the attendee.

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Scientific and practical informationScientific and practical information GGThe programme of WC7 has been developed around specific day themes as follows:Day 1, August 31: Innovative technologies, concepts and approachesDay 2, September 1: Areas of animal useDay 3, September 2: Progress in life science domains(Half) Day 4, September 3: 50 years after Russell & BurchDuring the first three days, the programme is organized in:Morning Sessions: held from 08:00 to 09:00Plenary Sessions: held one in the morning and one in the afternoonBreakout sessions: 2,5 hour-sessions running in parallel on specific topicsExtra-breakout sessions: 1 hour-sessions running in parallel focused mainly on international projects updatesLunch sessions: 2 per day, successivelyFaculty (Chairpersons, Speakers) and Oral Communication PresentersPlease locate your Session Room in due time, in order to be there at least 5 minutes before the session starts.Please respect the timing given to each presentation, in order to allow a smooth management of the Sessions.Technical information for Speakers and Oral Communication PresentersEach Room will be provided with a laptop and beamer and will be connected by LAN network to the Slide Centre.The use of personal laptop should thus be avoided, since it may cause delays and technical problems.Exceptions have to be agreed in advance by the WC7 Organizing Secretariat.Speakers are kindly requested to go to the Slide Centre at least 2 hours before the beginning of their session - whennot possible the day before - in order to have their presentation copied and transferred via LAN to the relevantSession Room.Posters exhibitionAccepted abstracts are displayed in the CongressVenue from Sunday, August 30 toThursday,September 3 in the followingAreas:Main Lobby LevelMichelangelo Room - Poster Area 1Galleria San Pietro - Poster Area 2Corridor Monte Mario - Poster Area 3Garden Lobby LevelCorridor Cavalieri - Poster Area 4Corridor Belle Arti - Poster Area 5Please refer to the section: Poster Exhibition (page 44-61) to know where the different Poster categories are located.Posters should be set up preferably from 16:00 to 20:00 hrs on August 30 or as soon as possible after arrival of thepresenter(s) and removed on September 3.Authors are responsible for the set up and dismantling of their poster(s): the WC7 Organizing Secretariat will provide forthe necessary stationery.Guidelines for posters displayPoster size must be cm 70 (width) x cm 100 (height). Your poster has been given a number and should be fixed on theboard marked with the same number. Authors are kindly suggested to stand by their poster(s) during lunch time of theday the theme of which corresponds most closely to the subject of the poster for possible questions by the congressparticipants. Authors could also leave a note to inform when they will be available at their poster. All presentationsmust be in English.Please note that poster authors should be registered participants.Oral communicationsSome of the abstracts submitted for oral presentations have been selected for inclusion in the Breakout Sessions of theCongress.They are marked by the Abstract ID number.Abstract BookThe Abstract Book edited by ALTEX contains all accepted abstracts from submissions and from the Invited Speakers.A copy is included in the Congress Kit, which will be given to all Participants at the Registration Desks.Demonstration of Altweb/ALTEX websiteCAAT has organized a demonstration of the Altweb/ALTEX website, scheduled for Tuesday, September 1 from 12:30 to14:30 in the Exhibition Area (Garden Lobby level, in front of the Registration Desks).

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General informationGeneral informationCongress VenueROME CAVALIERI HOTELThe Waldorf Astoria CollectionVia A. Cadlolo, 101 - 00135 Rome, Italyph. +39 06 35091website: www.romecavalieri.comThe Rome Cavalieri offers to its guests a complimentary daily shuttle service to the City Centre (PiazzaBarberini).The time table is available from the Hotel Concierge and at the WC7 Organzing Secretariat.

Congress rooms and areas:

WC7 Organizing Secretariat (AIM Group)The pre-registration and on-site registration desks, accommodation and travel desks, are at the disposal ofparticipants as follows:SUNDAY,AUGUST 30 16:00-19:00 hrsMONDAY,AUGUST 31 07:00-18:30 hrsTUESDAY, SEPTEMBER 1 07:00-18:30 hrsWEDNESDAY, SEPTEMBER 2 07:00-18:30 hrsTHURSDAY, SEPTEMBER 3 08:30-13:30 hrsAdmission & badgeEach participant which has paid his/her registration fee will receive a name badge.Name badges are used as passes. All participants, accompanying persons and official guests are kindlyrequested to wear their badge during all congress activities and social programme.Badges are distinguished as follows:Faculty: redDelegates (including abstract authors): transparentAccompanying persons: yellowExhibitors/Sponsors staff/press: green

Sightseeing for Accompanying personsAlthough the Congress has not forseen pre-organized tours or excursions, you will have the opportunity

to evaluate some customized proposals of guided walking tours: the Cultural Association EOS(www.eoscultura.it), based in Rome, whose scope is the promotion of cultural and artistic knowledge,

will be present at the WC7 Secretariat Desk and will offer a selection of sightseeing tours guidedby archeologists or art historians. Detailed programme is available at the EOS desk.Approximate price

for 2,5/3 hour tours is € 26,00 (€ 18,00 for young participants under 18) + costs for possibleentrance tickets.Tours may require a minimum attendance of 8 participants to be organized.

GARDEN LOBBY LEVELAuditorium (Congress sessions)Cavalieri 1, Cavalieri 2 (Congress sessions)Foyer (Exhibition)Garden Lobby (Registrations,Exhibition,Internet Point)Room Leonardo (InterNiche Multimedia Exhibition)Room Ellisse (Congress Sessions)Room Caravaggio (Slide Centre)Corridor Cavalieri (Poster Area 4)Corridor Belle Arti (Poster Area 5)Cloakroom

MAIN LOBBY LEVELRoom San Pietro (Congress sessions)Room Michelangelo (Poster Area 1)Room Terrazza Monte Mario (Congress Sessions)Galleria San Pietro (Poster Area 2)Corridor Monte Mario (Poster Area 3)

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Lunches and lunch couponsOn August 31, September 1 and 2, from 12:30 hrs to 14:30 hrs, box lunches are distributed free of chargeto Congress participants (Faculty, delegates, exhibitors).The relevant coupons are inserted in the plasticname badge holder.Attendances certificatesCertificates of attendance are handed out on request to all registered participants at the end of the meeting.Exhibition AreaA technical and publication exhibition will take place in the Congress Venue throughout the scientific works.The exhibition will follow the following timetable:SUNDAY,AUGUST 30 16:00-19:00 hrsMONDAY,AUGUST 31 09:00-18:30 hrsTUESDAY, SEPTEMBER 1 09:00-18:30 hrsWEDNESDAY, SEPTEMBER 2 09:00-18:30 hrsTHURSDAY, SEPTEMBER 3 09:00-13:00 hrs

Multimedia ExhibitionInterNICHE will organize a Multimedia Exhibition (ME) for education and training alternatives that willconsists of:- viewing and free trial of alternatives using InterNICHE Alternatives Loan System items;- POP (Pulsating Organ Perfusion) Trainer for surgical practice set up with ethically sourced organs fordemonstrations of live surgical training at the event;- detailed demonstrations and training in selected alternatives by InterNICHE trainers and by selectedproducers of alternatives;- possibility for all delagates to demonstrate their alternatives at a free workstationThe ME will be set up in the Room Leonardo.

InternetWI-FI Internet cards can be bought at the hotel Reception.WC7 Internet Point is available for all Congress attendees free of charge: users will be required to givetheir identity document and are asked to use a PC for not more than 15 minutes, in order to allow accessto everybody.LanguageThe official language of the WC7 is English. Simultaneous translation will not be provided.InsuranceThe Organizing Secretariat cannot accept liability for personal injuries sustained or for loss or for damageto property belonging to congress participants, either during or as a result of the congress. Please checkyour own insurance.BarInside the Congress venue several bars are available; they follow the Hotel rates.CloakroomA cloakroom is at participants’ disposal, for free. It is located in the Garden Lobby Level. Delegates arekindly requested not to leave their personal belongings after the closing time of each Congress day.

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Congress transportationWC7 has organized a private bus transportation service between the Congress hotels (see list below) andthe Congress Venue.The service is foreseen each day in the morning and at the end of the day sessions, and for all social events.For the detailed timetable, please refer to the flip charts shown in the hotels lobby.This service is reserved to the attendees who have reserved through the WC7 official Organizing Secretariat.

No-smokingSmoking in the congress area is not allowed.Mobile phoneParticipants are kindly requested to switch their mobile phones to silence mode or turnedoff in the rooms where scientific sessions are held.

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It is hard to say what is most breathtaking about the Eternal City, the Vatican, the Forum, the Colosseum,just to name few of the most famous symbols of the Italian capital.This city is incomparable and part of its allure lies in its history, which proceeds side by side with legend.Through the course of the centuries, the city has acquired a unique appearance, which makes it possible forthe visitor to stroll through twenty-five centuries of history, architecture and art.Public TransportationThe cost of the surface and underground transportation is the same.Tickets of different time limit and itinerary are available at ATAC counters, news-stands, tabacconists andautomatic ticket dispensers, but not in buses. All tickets must be validated at the beginning of the first tripeither at underground station or on buses.Please note that tickets must be bought before boarding the bus/metro.MetroThe 2 line system has an X shape, with both lines meeting at the Central Railway Station Termini.The first line, line B south goes from Laurentina to Rebibbia.Line A links Anagnina, in the south, withBattistini, in the northwest.The metro operates from 05:30 until 23:30 hrs (0:30 hrs on Saturdays) every 3-4 minutes during rushhours, every 5-6 during the day and every 8-10 minutes early morning and late evening.TaxiLicensed taxis are white, have a name, an identification number, and are equipped with a taxi-meter.Do not trust people who approach you offering private taxi service. In the city centre there are a numberof taxi-ranks, like for example in Piazza della Repubblica, Piazza Venezia and Largo Argentina.A taxi can becalled at the following telephone numbers: 06.3570, 06.4994, 06.5551.Please remember that in this case the taxi-meter is turned on when the taxi picks up the call. Because ofthe frequent changes made to the rates, you should read carefully the information shown inside the taxi.CurrencyThe official currency is the Euro.Bank and exchangeBanks are open from Monday to Friday, from 08:30 to 13:30 hrs and some of them again in the afternoonfrom 14:45 to 15:45 hrs (afternoon opening may vary depending on the bank).Banks are closed on Saturday and Sunday. Many banks have automatic currency exchange machines that alsotake credit cards. Most hotels, restaurants and shops accept foreign currency and major credit cards.Post officesThe opening of the post offices are:Monday to Friday from 08:30 to 15:30 hrs, Saturday from 08:30 to 12:00 hrsWeatherAugust and September weather in Rome is generally hot. The temperature can rise over 35°.ElectricityVoltage in Italy is mostly 220 volts - 50 Hz. Foreign appliances could require an adapter.TippingService is usually included in the bill in bars and restaurants, but tips are welcome.Useful NumbersAmbulance: dial 118Police: dial 113Fire brigade: dial 115

About RomeAbout Rome

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Registration feesFull participation Euro 525,00 (VAT included)Students/ Young Scientist* Euro 375,00 (VAT included)Accompanying person Euro 300,00 (VAT included)Daily registration Euro 150,00 (VAT included)* Students/young scientists under 30 are eligible when presenting an official confirmation of enrolment in ascientific institutionWhat is covered by the fee?The Full Participation includes:- congress kit (bag, badge,ALTEX abstact book and final programme)- admission to all scientific events- box lunches on August 31, September 1 and 2- the Welcome Cocktail and Get-Together Party on Sunday- the Garden Party on Monday- the Congress Dinner on WednesdayThe registration fee for Accompanying Persons includes:- the opening and closing ceremony- the Get-Together Party on Sunday- the Reception on MondayThe registration fee does not include accommodation and travel costs.PaymentThe payment of the registration fee should be made at the “New registration” desk by one of the followingmethods:- credit card (American Express,Visa, Mastercard and CartaSI are accepted)- cashThe relevant original invoice will be sent after the Congress. Participants are requested to provide full detailsfor the invoice heading and delivery.

Social programmeWelcome Cocktail and Get-Together PartySunday, August 30A Cocktail Reception will give the welcome to all WC7 attendees. Location is Room Belle Arti and thebeautiful gardens of the Rome Cavalieri.Join for a drink around the pool and listen to the music of LEVEL EYE…“…a two person group with a full band sound, they present themselves with a repertoire of originals andcovers in a Rhythm & Blues, Soul style.The music of Ivy and Ian is not something that goes in one ear and out the other. It’s music that’s intenselyalive; this profound emotion can be felt…”This event is included in all Registration fees. Extra tickets can be bought at the costof Euro 42,00 per person (VAT included).

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Garden PartyMonday, August 31A buffet of different Pastas and Pizzas for all tastes - we are in Italy of course… Although the location is thesame as the Cocktail Reception, the ambience and the music, by PLAY IT SAFE band, will be rather different!This event is included in all Registration fees. Extra tickets can be bought at the costof Euro 60,00 per person (VAT included).

Congress DinnerWednesday, September 2Location:Villa Miani (Via Trionfale, 151 - Rome)Placed on the slopes of Monte Mario,this beautiful mansion built at the beginningof the 20th Century, offers a unique viewon the city of Rome.Residents in the Cavalieri Hotel canreach the location walking for less than1 km in the wonderful age-old park.Transfers will be organized from/tothe other Congress hotels.This event is included in the Full and Student/Young scientist Registration fees.Tickets can be bought at the cost of Euro 75,00 per person (VAT included).

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August 27-28 – San Servolo Island, Venice

Workshop: Genomics in cancer risk assessmentCo-organized by the carcinoGENOMICS Program, ECVAM, HESI Application of Genomicsto Mechanism-based Risk Assessment Technical Committee, Netherlands Genomics InitiativeDetails on www.hesiglobal.org

August 30 – Rome Cavalieri Hotel

14:00-17:00Room: EllisseSymposiumFrom tissue engineering to alternatives: research, discovery and developmentCo-organized by CELLTOX (Associazione Italiana di Tossicologia in vitro) and ESTIV(European Society of Toxicology in Vitro)

14:00-17:00Room: Monte Mario TerraceJoint meeting of Altweb Project Team and 3Rs CentersOrganized by the Johns Hopkins Center for Alternatives to Animal Testing (CAAT)

17:00-20:00Room: San PietroAnimal Protection Satellite MeetingBringing together International Animal Protection OrganisationsOrganized by the Humane Society of the United States (HSUS) and Eurogroup for Animals

Pre-Congress Satellite MeetingsPre-Congress Satellite Meetings

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MORNING SESSIONS08:00-09:00Animal Welfare Associations (MS3)Room: EllisseChair: Sonja vanTichelenThe foundation Animalfree Research:33 years of replacing animal experimentsStephanie SchindlerThe American Society for Prevention ofCruelty to Animals (ASPCA) and 3R initiativesSafdar A. KhanEurogroup for AnimalsKirsty ReidThe HSUS and the 3RsMartin L. StephensVereniging Proefdiervrij(Dutch Society for Replacement of Animal Testing)Marja ZuidgeestThe work of the RSPCA - laboratory animalsMaggy Jennings, Barney Reed

08:00-09:003R centers 1: Asian regulatory affairsin animal welfare and alternatives (MS1)Room: Monte MarioChair: Miki Tsutomu KurosawaThe continuation of Asian activitiesof alternative research fromWC6 toWC7Miki Tsutomu KurosawaAccreditation of animal experimentsby Japan Health Science FoundationYasuo OhnoTwo laws about laboratory animal welfareand use in KoreaJae-Hak ParkLaboratory animal welfare and ethical reviewof animal experiments in Chinese RegulationsRuizhang Rong

08:00-09:00Industry activities 1 (MS2)Room: San PietroChair: Betsy N. MerrillBuilding a 3Rs corporate cultureat Johnson & JohnsonSheldon SloanProcter & Gamble’s approachto the developmentof alternativesMark LafranconiThe COLIPA programmeon alternativesOdile de Silva

OPENING CEREMONY09:05-09:15Room: Auditorium

PLENARY SESSION 1 (PL1)09:15-10:00Room: AuditoriumChair: Horst SpielmannThe U.S.“Tox21 Community” and the futureof toxicology testingRaymond R.Tice

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BREAKOUT SESSIONS10:10-12:40In vitro technologies (BS9)Room: AuditoriumChair: Horst SpielmannThe development of tissue engineering modelsfor the study of human liver diseasesBrianThomson(ABS 39) Advances in the development of anin vitro high-throughput test system to evaluatethe carcinogenic potential of chemicalsPablo Steinberg et al.(ABS 266) In vitro assays of ventricularcardiomyocytes as screening tools in drugdevelopment and safety pharmacologyElke Guenther et al.3D tissue engineered models of skinand oral mucosa for investigation of normaland abnormal epithelial biologySheila MacNeil(ABS 483) Using clinically relevant celecoxibconcentrations to identify potential biomarkersin human colon polyp cellsEugene Elmore et al.

10:10-12:40Integrated approaches (BS1)Room: Cavalieri 1Chair: Gerrit SchüürmannConcept of integrated testing strategies(ITS) for chemical safety assessmentGerrit SchüürmannExposure based waiving under REACHTheo VermeireChemical category evaluation as non-testcomponent of ITSOvanes G. Mekenyan(ABS 64) Tiered approaches to the useof alternative approaches to animal testingfor the safety assessment of cosmetics:skin and eye irritation, genotoxicityJulia Scheel et al.Use of TTC under REACH - AreTTCsapplicable to industrial chemicals?Inge MangelsdorfWeight-of-evidence based ITS approachin human toxicologyDinant KroeseTowards a theory of tiered testingas approach within ITSChristina Ruden

10:10-12:40High throughput technologies (BS3)Room: Cavalieri 2Chairpersons: Christopher P. Austin;Maurice P. WhelanThe role of quantitative high throughputscreens in toxicology testingChristopher P. AustinIHCP/ECVAM activities on high throughputand high content screening for hazard profilingof chemicals and nanomaterialsMaurice P. WhelanR&Ds of in vitro and in silico alternativesto animal testing under the strategicR&Ds of chemical risk analysis technologiesby the NEDO, JapanTakuya IgarashiOECD activities related to the internationaldevelopment and evaluation of high throughputtechnologies for hazard assessmentMichihiro OiBiokinetic factors in in vitro systems: analyticaltools to analyse the freely available concentrationBas J. Blaauboer

10:10-12:40In silico models (BS7)Room: EllisseChair: Joanna JaworskaIntroduction to the sessionJoanna Jaworska3R systems for biomedicaldiscovery accelerationLaurence HunterIn silico prediction of the toxicpotential of drugs and chemicalsAngeloVedaniPredicting CYP-mediated xenobioticmetabolism in human skinGabriele CrucianiThe cyberbiohybrid lung: a prototypecell-based computational model forpulmonary drug developmentGus Rosania(ABS 240) The patient specific virtualcardiac ventricular wall: quantitative predictionof pro-arrhythmic effects of anti-arrhythmicsArun Holden et al.

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10:10-12:40Chemical and physical methods (BS2)Room: Terrazza Monte MarioChair: Siegfried MorathOptical biochipsStéphane FollonierLiquid chromatography-mass spectrometrydetection of marine biotoxins in seafood:a technique to reduce mouse bioassaysJean Pierre LacazeSingle molecule force spectroscopy of specificblood protein interactionsMark H. Schoenfisch(ABS 314) Saving animals by in vivoimaging with PET and MRI: proposed innovationsin developmental neurotoxicty testingDidima De Groot et al.

EXTRA BREAKOUT SESSIONS10:10-11:10Status report on PREDICT-IV (EB1)Room: San PietroChair: Christof J. BurekPREDICT-IV a new approachfor integrated in vitro toxicity testing into theearly stages of drug developmentChristof J. BurekCell culture approaches chosen to predictadverse effects of therapeutic compoundsWalter PfallerThe crucial role of biokinetics in in vitro testingEmanuela TestaiHow to integrate in vitro PK/PD informationfor toxicity predictionFrédéricY. Bois

11:30-12:30Status report on CAESAR (EB3)Room: San PietroChair: Emilio BenfenatiCAESAR’s approach for alternative in silicomethods for REACHEmilio BenfenatiThe in silicomodel for bioconcentration factor (BCF) in fishAlessandra RoncaglioniThe in silico model for mutagenicityGiuseppina GiniThe in silico models for carcinogenicity, skinsensitization and developmental toxicityJacques R. ChrétienFormation of mechanistic categories and localmodels to facilitate the prediction of toxicityMarkT.D. Cronin

12:30-14:50LUNCH - POSTER EXHIBITION

LUNCH SESSIONS12:40-13:40Databases: progress report (SL1)Room: San PietroChair: Barbara GruneWealth and diversity of the 3Rs onlineMaryW.WoodThe AnimAlt-ZEBET Database: a uniqueresource for comprehensive and value-addedinformation on 3Rs alternativesDaniel ButzkeAltweb + ALTEX: a 21st Century unionMichael M. HughesAnimalWelfare Information Center (AWIC):providing information for improved animal careand use in research, teaching and testingKristina M.AdamsAltTox.Org: connectingstakeholders on issues concerning non-animalmethods of toxicity testingMartin L. Stephens

13:40-14:40Good Cell Culture Practices (SL2)Room: San PietroChair: Gerhard GstraunthalerThe Bologna Statement on Good CellCulture Practice (GCCP) - 10 years laterGerhard GstraunthalerQuality Control in cell culture:simultaneous authenticationand cross-contamination detectionWilhelm G. DirksStandardisation in the Cultureof Stem Cell LinesGlyn StaceyGCCP initiatives in JapanHajime Kojima

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BREAKOUT SESSIONS14:50-17:20Omics and systems biology (BS4)Room: AuditoriumChair: Jos KleinjansOpening wordsJos KleinjansGene expression profiling as a tool to developalternatives to animal toxicity testingJoost H.M. van DelftMetabolic profiling as a tool in biomarkerresearch and systems biologyHector KeunEvaluation of chemically inducedepigenetic alterations through whole genomeDNA methylation analysisIgor PogribnySystems modeling as an iterativeapproach towards understanding mechanismsof toxicity and carcinogenesisRalf HerwigUsing systems biology to develop thegene-environment disease interactome for humansChristopher J. Portier

14:50-17:20Non-invasive technologies (BS5)Room: Cavalieri 1Chairpersons:Andreas Hess; Kay BruneIntroduction to the sessionAndreas HessBioimaging of laboratory animals: reduce animalsnumbers, increase animal to human translationMyrtle Davis-MillinNeuroimaging techniques and applications to painperceptionPaul L. FurlongNon-invasive FMRI of the pain systemin rodents under mild anesthesiaAndreas HessNon invasive imaging of small animalsin biomedical research: multimodal imagingwith PET and MRTBernd Pichler(ABS 109) Urinary metabolites asnon-invasive biomarkers for the pleiotrophiceffects of peroxisome proliferator activatedreceptor-alpha agonists in ratsRaymond Poon et al.(ABS 313) Ultrasonic vocalizations(USV) in rat pups.An animal friendly markerfor neurotoxicity during developmentDidima De Groot et al.

14:50-17:20Non-vertebrate models (BS6)Room: Cavalieri 2Chair: Holger Knaut(ABS 327) Dictyostelium amoebae:a model host to study infectious bacteriaPierre Cosson et al.(ABS 10) Reducing animal-based drugtoxicology testing using a single cell organismJ. Mukanowa et al., presented by Robin S.B.WilliamsMedium and high-throughput toxicity screensusing C. elegansJonathan H. Freedman(ABS 47) Galleria mellonella as a flexiblemini-host system to study virulenceof aspergillus fumigatus and efficacy of theantifungal drugs used in treatmentJoanne L. Slater et al.Drosophila melanogaster:an invertebrate genetic model system forcomplex-disease screening and testingLinda L. Restifo14:50-17:20Databases: scientific approaches (BS8)Room: EllisseChair: Ivan ArisiData integration in the life sciences:from data to networks, models and phenotypesShankar SubramaniamMining on expressed sequences:examples from plant genomicsMaria Luisa ChiusanoGo3R, http://www.go3r.org, the first semanticsearch engines for alternative methodsMichael SchroederData mining and knowledge extractionfrom scientific data basesGiovanni Felici(ABS 560) Chemical safety assessment-evaluationof in silico predictive systemValentina Karan et al.

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14:50-17:20Current and evolving conceptsfor the validation of safetyassessment method (BS10)Room: Terrazza Monte MarioChairpersons: Hajime Kojima;Joachim Kreysa;William S. StokesValidation of innovative technologiesand strategies for regulatory safety assessmentmethods: challenges and opportunitiesWilliam S. StokesValidation of innovative test methodsfor safety testing: drawbacks and advantagesof Japanese validation studiesHajime KojimaValidation of innovative test methodsfor safety testing: ECVAM’s experienceand lessons learnedJoachim KreysaThe use of test method performancestandards to expedite validation of innovativeand improved versions of test methodsand testing strategiesMarilyn L.WindUse of results of alternative testing methodsfor regulatory purposes in the EUFinn PedersenInternational harmonisationand regulatory acceptance of OECD testguidelines and validation principlesPatric Amcoff

EXTRA BREAKOUT SESSIONS14:50-15:50Status report on EvidenceBased Toxicology Project (EB2)Room: San PietroChair: Sebastian HoffmannEvidence-based toxicology: breakingthe chain of uncertainty?Claudius GriesingerToxRTool: a tool to assess reliabilityof toxicological dataSebastian Hoffmann

16.00-17.00Update on ICH and VICH Progress (EB12)Room: San PietroChair: Umberto AgrimiVICH:Approach to the 3Rs with particular referencetoTarget Animal Safety guidelinesTim RowanMaintenance of internationally harmonised (ICH)safety guidelines taking into consideration new dataand approaches including the 3RsRepresentative of EFPIA (European Federationof Pharmaceutical Industries and Associations)

PLENARY SESSION 2 (PL2)17:30-18:15Room: AuditoriumChair: Martin L. StephensAnimal health and welfare in the food chain:food for thoughtHerman B.W.M. Koëter

20:30-23:00Garden Party

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MORNING SESSIONS08:00-09:00Associations (MS6)Room: EllisseChair: Luigi Campanellaecopa: realisations and future perspectivesVera RogiersESTIV, a bridge between scientists workingon alternative methods in EuropeGreet E.R. SchoetersCELLTOX -The Italian Associationfor in vitroToxicologyIsabella De AngelisChemistry contribution to the reductionof the experiments on animalsLuigi Campanella

08:00-09:003R centers 2 (MS5)Room: Monte MarioChair: Carol J. HowardThe Nc3rs - Supporting scienceand animal welfareVicky RobinsonA proposal of establishing a BrazilianCenter forValidation of AlternativeMethods (BRACVAM)Octavio PresgraveA National Center for animal alternativesin India: the Mahatma Gandhi - DoerenkampCentre for Alternatives to Animal usein Life Science Education (MGDCAALSE)M.A.Akbarsha

08:00-09:00Educational activities (MS4)Room: San PietroChair: Jan van derValkEstablishment of dedicatedchairs for alternative methods asstrategy to promote the 3RMarcel LeistCAAT’s humane science policy,outreach & academic programsBetsy N. MerrillThe 3R's in veterinary medical educationFrauke Ohl

PLENARY SESSION 3 (PL3)09:10-09:55Room: AuditoriumChair: Coenraad HendriksenBrueghel's two monkeys: passing the finalexam in the history of mankindIngrid Newkirk

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BREAKOUT SESSIONS10:00-12:30Chemicals and pesticides (BS12)Room: AuditoriumChair: Neil G. CarmichaelREACH and the need for IntelligentTesting Strategies (ITS)Kees van LeeuwenReduction of ‘fish’ use in environmentalhazard and risk assessmentGraham F. WhaleRegulatory requirementsfor pesticide and biocide testing and scopefor alternatives apporachesManfred LiebschInitiatives to decrease redundancy in animaltesting of pesticidesBennard van RavenzwaayStrategies for reproductive anddevelopmental toxicity assessmentGeorge Daston(ABS 544) An examination of newchemical regulation policies as a meansto revolutionize toxicity testingKristie M. Sullivan et al.

10:00-12:30Basic research (BS11)Room: Cavalieri 1Chair: Joanne ZurloConcept of the 3Rs in basic researchJoanne ZurloIncorporating the 3Rs into basicmechanistic toxicology researchJames D.YagerThree-dimensional human tissue structuresto study cellular differentiationPaul GeneverViable alternatives: choosing the properexperimental model for neuroscience studiesRandall J. NelsonRestoration and maintenance of the in vivo-likehepatocellular homeostatic balance:key for the establishment of long-termliver-based in vitro modelsMathieuVinken(ABS 492) Molecular and genomiccharacterization of estrogenic effectsof 3-methylcholanthrene (3-MC)Ingemar Pongratz et al.

10:00-12:30Pharmaceuticals (BS14)Room: Cavalieri 2Chair: PhilippeVanparysIntroduction to the sessionPhilippeVanparysWhat did we learn up to now by a projectsuch as START-UP?Vera RogiersAdherence to the 3R principles in the ICH processPeter KasperImplementation of the 3Rs in the EuropeanPharmaceuthical IndustryFreddyVan GoethemCurrent situation of alternatives for animaltesting in Japanese pharmaceutical industryTakuja IkedaHow we use alternatives in the US and whyRobert E. Chapin(ABS 558) Human tissue pharmacology:a true animal alternativeSarah Lynagh et al.

10:00-12:30Genetically modified organisms (BS16)Room: EllisseChair: Harry A. KuiperSafety assessment of GMOs and derivedfood and feed: the role of animal feeding trialsand alternative methodsHarry A. KuiperSafety testing of GM crops for human and animalconsumptionBruce G. HammondLatest developments in allergenicitytesting of GMOsJean MichelWalOmics approaches for the safetyassessment of GMOsHoward Davies

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10:00-12:30Education and training (BS19)Room: Terrazza Monte MarioChairpersons: Jan van derValk;Bert van ZutphenInstitutional animal user training in CanadaClément J.T. GauthierEducation and training for researchers inlaboratory animal science in AustraliaMargaret A. RoseAsian trend of education and training inlaboratory animal medicine for refinementMiki Tsutomu KurosawaFELASA Category C courseBryan HowardEducation and training for researchers inlaboratory animal science in the United StatesMarilyn J. Brown

EXTRA BREAKOUT SESSIONS10:00-11:00Status report on ICATM (EB4)Room: San PietroChair: Marilyn L.WindWhat is the International Cooperation onAlternativeTest Methods (ICATM) and what is its role?Marilyn L.WindICATM’s importance for ECVAM and the 3R’s in the EUJoachim KreysaCurrent and future contributionsof NICEATM and ICCVAM to the ICATMWilliam S. StokesJaCVAM’s role in the 3R’s and ICATMHajime KojimaHealth Canada’s role in ICATMDavid Blakey

11:10-12:10Status report on EPAA (EB5)Room: San PietroChairpersons: Georgette Lalis;Odile de Silva; Cornelis BrekelmansEPAA: what is new?Georgette LalisEPAA: paving the way towards new perspectives on safetyGianni Dal NegroAcute toxicity testing: an EPAA analysis across sectorsSally Ann RobinsonIn vitro metabolism test systems as essential partsof integrated test strategies for long-term toxicitiesreinforced by EPAA's joint effortsSandra CoeckeConclusionsOdile de Silva

12:30-13:45LUNCH - POSTER EXHIBITION

LUNCH SESSIONS12:30-13:302009 developments in the fieldof alternative methods (SL3)Room: San PietroChair: Alan M. GoldbergThe EU / COLIPA call for research proposals forsystemic toxicityJuergen BuesingThe Orange House PartnershipHerman B.W.M. KoëterTheTransatlantic Think-Tank forToxicology (t4)Marcel LeistCAAT EuropeThomas HartungFICAM -The Finnish Centre forAlternative MethodsTuula Heinonen

13:30-14:30Recent progress and future directions inthe validation and regulatory acceptanceof alternative test methods that reduce,refine and replace animal use (SL4)Room: San PietroChairpersons:William S. Stokes;Hajime Kojima; Joachim KreysaRecent progress and future directionsat NICEATM-ICCVAM: validationand acceptance of alternative methodsthat reduce, refine and replace animal useWilliam S. StokesRecent progress and futuredirections at JaCVAMHajime KojimaRecent progress and futuredirections at ECVAMJoachim Kreysa

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BREAKOUT SESSIONS14:45-17:15Cosmetics (BS13)Room: AuditoriumChair: Odile de SilvaIntroductionOdile de SilvaChallenges and progress in thedevelopment of in vitro assays for eyeirritation safety evaluation: a Colipa updatePauline McNameeThe COLIPA strategy foranimal-free genotoxicity testingPaul L. CarmichaelSkin sensitization: the COLIPA strategyfor developing and evaluating non-animaltest methods for risk assessmentGavin M.C.K. MaxwellThe use of in silico/QSAR approachesto predict toxicity and fateOvanes G. MekenyanExperience on product safetyassessment within the cosmetics industryPascal BertheRound TableUse of alternative methods in the safetyevaluation of cosmetic productsRobert BronaughInternational regulatory acceptancefor non-animal alternatives in the areaof cosmetics regulationLaurent SellèsRegulatory requirements for cosmetics inthe EU and status of non-animal testsVera RogiersReview of safety evaluation of cosmeticingredients using Quasi-drugHajime KojimaPanel discussion

14:45-17:15Vaccines and biologicals (BS18)Room: Cavalieri 1Chairpersons:Marlies Halder;Mario Landys Chovel Cuervo3R trends and opportunities in vaccinequality control: an overviewCoenraad HendriksenPotency testing of rabies vaccine for humanand veterinary use, possibilities for alternativesLukas D. Bruckner;Wlamir Correa de MouraIn vitro tests for the assessmentof clostridial vaccine antigensKeith RedheadZEBET expert meeting on “Alternativemethods to replace the LD50 potency testin botulinum neurotoxin product testing:scientific and legal challenges"Sarah Adler(ABS 358) Transgenic biopharming:ethical, scientific and safety considerationsNirmala Bhogal et al.(ABS 372) The development of new adjuvantswith minimal side-effectsL. van Straalen et al., presented by Jeffrey J. Bajramovic

14:45-17:15Food improving agents (BS15)Room: Cavalieri 2Chair:Abigail JacobsIntroductionAbigail JacobsA detailed description of QSAR andTTCand their application to a variety of toxicologyendpoints for food additivesKirk B.ArvidsonIn vivo approaches to simultaneouslyassess both genotoxicity and carcinogenicityof food additivesAkiyoshi NishikawaAn industry perspective on alternativemethods to establish level of safety concernof food chemicals in absence of sufficienttoxicological dataManuel Dominguez Estevez

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14:45-17:15Nanomaterial toxicity testing (BS17)Room: EllisseChairpersons: Vicki Stone;Roel SchinsIntroductionVicki StoneCell culture models of the epithelialairway barrier to study the toxic potentialof nanoparticles: comparison to in vivo modelsBarbara Rothen-RutishauserGenotoxicity of nanoparticles - comparisonof in vivo and in vitro modelsRoel SchinsUse of in vitro and inhalation models forassessment of nanoparticle effects on lung cellsBrooke MossmanUsing in vitro models to predictthe in vivo pathogenicity of fibres and highaspect ratio nanoparticlesVicki Stone(ABS 69) Implications of action plansand research programmes towards replacinganimal testing in nanotechnologyUrsula Sauer(ABS 445)Alternative thinking on hazardassessment of new materials: nanomaterials and 3R’sCyrille A.M. Krul et al.

14:45-17:15Animal use policies (BS20)Room: Terrazza Monte MarioChair: Roman KolarAnimal experiments - Criteria for ethicalevaluation and ethical limitsJörg LuySpecial protection for primates - the needfor faster progressMaggy JenningsApplying the information gained from animalharm categorization systems - Case studyof Canada's categories of invasivenessGillian G. GriffinWhy should mammalian fetusesbe included in regulations protecting animalsduring experiments if they do not becomeconcious until after birth?David J. Mellor(ABS 9) Reviewing the reviews: an analysisof the process of ensuring regulatory compliance inthe use of animals in science in New ZealandVirginia M.Williams et al.

EXTRA BREAKOUT SESSIONS14:45-15:45Status report on ReProTect (EB6)Room: San PietroChair: Michael SchwarzReProTect: a 6th frameworkprogram aiming to develop new in vitrotests in reproductive toxicologyMichael SchwarzResearch area I: fertilityGiovanna LazzariResearch area II: implantationand placental functionLennart DenckerResearch area III: prenataldevelopmentHorst SpielmannResearch area IV: cross cuttingtechnologiesAlberto Mantovani

16:00-17:00Status report on OSIRIS (EB7)Room: San PietroChair: Gerrit SchüürmannIntroduction to the OSIRIS projectGerrit SchüürmannIn vitro toxicological alertsin ecotoxicological hazard assessmentHelmut SegnerDeveloping strategies for integratingin vitro, in vivo and in silico informationin mutagenicity/carcinogenicityRomualdo BenigniBayesian evaluation of non-animalinformation to support decisionmaking - skin sensitization test caseJoanna Jaworska

PLENARY SESSION 4 (PL4)17:25-18:10Room: AuditoriumChair: Marcel LeistMetabonomics-driven top-down systems biology:techniques and applicationsJohn C. Lindon

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MORNING SESSIONS08:00-09:00Industry activities II (MS8)Room: EllisseChair: Cornelis BrekelmansAssuring safety without animal testing:Unilever’s ongoing research programme todeliver novel ways to assure consumer safetyCarlWestmorelandProviding solutions for industry, regulatorsand the animal protection communityRodger D. CurrenThe science strategy of the European Centrefor Ecotoxicology andToxicology of Chemicals,ECETOCDavid OwenThe economics of animal testingAnnamaria Bottini

08:00-09:003R centers 3 (MS9)Room: Terrazza Monte MarioChair: Michael M. HughesCAAT: a 3Rs Center for the 21st CenturyCarol J. HowardANZCCART’s publication strategy: maintain,update and expandGeoffry DandieThe new 3Rs program atthe Canadian Council on Animal CareGillian G. Griffin

08:00-09:00Lessons learned from the validationand potential regulatory applicabilityof in vitro alternative pyrogen tests (MS7)Room: San PietroChairpersons: Leonard Schechtman;Suzanne C. FitzpatrickOpening remarks and speaker introductionLeonard SchechtmanDevelopment, purpose and importance of in vitropyrogenicity testsMardas DaneshianIn vitro pyrogenicity tests - Lessonslearned from the European validation studyand ESAC peer reviewMarlies HalderICCVAM review and evaluation of pyrogenicity testRichard McFarlandThe Monocyte ActivationTest (MAT) for pyrogensin year zero of its regulatory acceptanceThomas Montag-LessingWrap-up and closing remarksSuzanne C. Fitzpatrick

PLENARY SESSION 5 (PL5)09:10-09:55Room: AuditoriumChair: Clément J.T. GauthierPredictive testing strategies: R&D achievementsand perspectivesJean-Roch Meunier

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BREAKOUT SESSIONS10:00-12:30Skin and eye toxicity I (BS21)Room: AuditoriumChair: Rodger CurrenIntroduction to the current regulatory statusof in vitro dermal toxicity assaysRodger CurrenContribution of post-validation activities in the areaof in vitro Reconstructed human Epidermis (RhE) skinirritation tests to the process of OECD acceptanceManfred LiebschAn iterative approach to completeregulatory acceptanceVera RogiersBuilding and tuning in vitro skin toxicityprotocols is driven by tissue model structureand test-application constraintsJosè CotovioMechanistic assays for in vitro prediction ofskin sensitization: integrating biological andchemical measurements in a holistic approachAndreas NatschIn vitro phototoxicity studies withreconstructed human skin equivalentsHelena Kandarova(ABS 173) Assuring safety without animal testing:skin allergy case studyCameron Mackay et al.Closing remarksPauline McNamee

10:00-12:30Systemic toxicityand target organs (BS23)Room: Cavalieri 1Chair:Walter PfallerIntroductory remarks to the sessionWalter PfallerBiokinetic considerations in the use ofin vitro systems for estimating systemic toxicityBas J. BlaauboerHow to evaluate the target organ toxicitiesof cyclosporines? Lessons learned from in vitromodels applied in drug developmentArminWolfEvaluation of organ-specific toxicity with Integrateddiscreet Multiple Organ Co-culture (IdMOC) systemAlbert P. LiIn vitro models to study pulmonary absorption andclearance of drugs and (nano)particlesClaus Michael LehrMolecular mechanisms of toxicityin human renal epithelial cellsPaul Jennings(ABS 485) Characterization of toxicologicaleffects on the energy metabolism after exposure toendocrine disrupting compoundsTine L.M. Hectors et al.

10:00-12:30Reproduction, developmentand fertility (BS25)Room: Cavalieri 2Chair: Elaine M. FaustmanIntroductionElaine M. FaustmanEstablishing an extended 1-generation study at theOECD level - successes and challengesRalph L. CooperThe ReProTect Framework Program:new innovative approaches for evaluatingfertilization, implantation and prenatal developmentMichael SchwarzAssessment of the embryonic stem cell test andapplication and use in the pharmaceutical industryRobert E. ChapinThree-dimensional gonocyte-sertoli cell co-culture:characterizing systems based omic responses within vivo testis gene expression profilesXiaozhongYu(ABS 357) Modification and evaluation of thechicken embryotoxicity screening test (CHEST)as in vitro test system for embryotoxicitySusanne N.E. Boehn et al.(ABS 28) Evaluating teratological findings fromalternative developmental toxicity assay used inconjuction with traditional in vivo rodent studiesBelenTornesi et al.Summary and final discussionElaine M. Faustman

10:00-12:30Disease models (BS26)Room: EllisseChair: Jeffrey EverittIntroduction to session on disease models:balancing need with opportunityJeffrey EverittIn vitro models of skin cancer usinghuman cells and tissueMike P. Philpott(ABS 38) A cell-based chronic wound bioassayto replace unnecessary animal experimentationPhil Stephens et al.Refining clinical care in animal disease modelsKarl A.AndrutisEfforts to refine psychiatric disease modelsGianni Dal Negro

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10:00-12:30Immunology (BS29)Room: Terrazza Monte MarioChair: Erwin L. RoggenIntroduction: the challengesof in vitro immunotoxicologyErwin L. RoggenInnate immune mechanisms in contactdermatitis and the resulting responsesStefan F. MartinAnalyzing immune mechanismsunderlying multiple sclerosisDaniel AltmannFrom the 3Rs of animal studies to the 3Rsof the MIMIC system: Representative, Reliableand ReproducibleWilliam L.Warren(ABS 387) Search for biomarkersto evaluate immunotoxicity of chemicals and todevelop cell-based screening methodsSetsuya Aiba et al.(ABS 521) Uromodulin exhibits pro-inflammatoryproperties in an in vitro model of whole bloodactivation and neutrophil migrationSinikka Prajczer et al.

EXTRA BREAKOUT SESSIONS10:00-11:00Status report on ACuteTox (EB8)Room: San PietroChair: Cecilia ClemedsonOverview of the ACuteTox projectand multivariate analysis of the in vitro dataCecilia ClemedsonStatistical analysis of the ACuteTox data:challenges and statistical approachesAnnette Kopp-Schneider

11:10-12:10Status report on ToxCast (EB9)Room: San PietroChair: Robert KavlockToxCast: using high throughput screeningto identify profiles of biological activityRobert KavlockToxMiner: relating ToxCast bioactivityprofiles to phenotypic outcomesRichard Judson

12:30-14:45LUNCH - POSTER EXHIBITION

LUNCH SESSIONS12:30-13:30Animal use policies: the futureof animal welfare legislation (SL5)Room: San PietroChair: Roman KolarThe revision of EU Directive 86/609/EEC on theprotection of animals used for scientific purposesSusanna LouhimiesThe need for a coherent and comprehensivestrategy on reducing animal testsSonja vanTichelen

13:30-14:30Vaccines: acceptanceof 3R methods (SL6)Room: San PietroChair: Roland DobbelaerAcceptance of 3R in testing of vaccinesfor human and veterinary use:industry’s view on current status andbottlenecks to move forwardRuurd StolpAcceptance of 3R in testing of vaccinesfor human and veterinary use:EDQM’s view as on current status andbottlenecks to move forwardJean-Marc SpieserAcceptance of 3R in testing of vaccinesfor human and use: WHO’s view on currentstatus and bottlenecks to move forwardRepresentative of theWorld Health Organisation

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BREAKOUT SESSIONS14:45-17:15Skin and eye toxicity II (BS22)Room: AuditoriumChair: Pauline McNameeECVAM Bottom-Up/Top-Down testing approach:testing strategy to reduce/replacethe Draize test and validation/regulatoryacceptance of in vitro assays: current statusValérie ZuangECVAM validation drives EpiOcularTMexpanded applicability domain for Europeanlegislation relevant test articles: successfulinternational pre-validationPatrick J. HaydenOptimization and pre-validation of an in vitrotest strategy for predicting ocular irritancyusing the SkinEthicTM Human Corneal Epithelial(HCE) model protocolNathalie AlépéeProspective validation study of in vitroreconstructed human tissue assays for detectionof acute eye irritationStuart J. Freeman(ABS 142) Development of new alternative methodfor eye irritation test using gel embedded culturemethodMegumi Nakashima et al.Combining alternative test methods topredict eye irritation potential: a practical approachbased on data mining techniquesEls AdriaensMeasuring depth of injury in an isolatedrabbit eye irritation test using biomarkersof cell death and viabilityJohnW. HarbellClosing remarksRodger Curren

14:45-17:15Genotoxicity and carcinogenicity (BS24)Room: Cavalieri 1Chairpersons: Makoto Hayashi;Raffaella CorviRe-defining in vitro genotoxicity tests forbetter performance - a report fromthe InternationalWorkshop on GenotoxicityLutz MüllerGenotoxicity testing in vivo: strategies toreduce the number of animals in mandatoryregulatory studiesPeter KasperCell transformation assays: current statusRaffaella CorviThe NEDO project on carcinogenicityNorihoTanaka(ABS 444) The use of ex vivo humanskin for safety testingCyrille A.M. Krul et al.(ABS 27) In vitro test, image analysis andstatistical classification: towards an integratedapproach for human carcinogenicity testingChiara Urani et al.

14:45-17:15Environmental science (BS27)Room: Cavalieri 2Chairpersons: Kristine Schirmer;Scott E. BelangerRecent advances in developing alternativesfor acute and chronic fish toxicity tests usingfish cell lines and embryosStefan ScholzIntegrating non-vertebrate toxicologicalinformation as a substitute to vertebrateenvironmental testingScott E. BelangerUse of mode and mechanism of action informationto support in silico prediction of eco-toxicityMarkT.D. CroninThe universal biomarker concept: usingnon-mammalian alternatives to protect theenvironment and human healthRichard D. HandyDevelopment and regulatory acceptanceof alternative OECD approaches and test guidelinesfor the protection of the environment and humanhealth - an international perspectivePatric Amcoff(ABS 301) The ILSI-HESI project on animal alterna-tive needs in environmental risk assessmentMichelle Embry et al.

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14:45-17:15Animal welfare science (BS28)Room: EllisseChair: David MortonWhen do mammalian young become sentinet?David J. MellorInternational perspectives on animal welfareMargaret A. RoseAnimal husbandry, standardizationand scientific validityHannoWürbelEnrichment, chronic stressand its impact on research dataJoe GarnerLess pain, same gain: combining science and welfareDavid Morton(ABS 538) Planning for refinement and reductionDerek Fry et al.(ABS 239) Improving the qualityof publications on animal experimentsto make systematic reviews possibleCarlijn R. Hooijmans et al.(ABS 163) Applicability of alternativeapproaches in food and feed risk assessmentat the European Food Safety AuthorityDaniela Maurici et al.

14:45-17:15Neuroscience (BS30)Room: Terrazza Monte MarioChairpersons: Pamela J. Lein;Alan M. GoldbergOverview of Developmental Neurotoxicity Testing(DNT): problems and approaches for minimizinganimal use and maximizing data collectionPamela J. LeinHuman neural progenitor cells for DNT testingEllen FritscheIn vitro models to study cell-cell interactionsthat influence developmental neurotoxicityLucio G. CostaA genetic model system for studying effects ofmutations and toxins on developing neurons in vitroLinda L. RestifoIn vitro high throughput analyses of cellularprocesses critical for neurodevelopmentWilliam R. Mundy(ABS 105) Gene expression as a sensitiveendpoint to evaluate developmental neurotoxicityinduced by pesticidesAnna K. Price et al.

EXTRA BREAKOUT SESSIONS14:45-15:45Status report on Sens-it-iv (EB10)Room: San PietroChairpersons: Erwin L. Roggen;Hans-UlrichWeltzienThe current knowledge about the biologicalprocesses that occur when tissue is exposed tosensitizing materialsErwin L. RoggenPCLS: from learning about in vivo toreduction and replacementArmin BraunPotential EC and DC-based tests forassessing sensitisationSue GibbsT-cell priming and amplification:exploiting key events in contact sensitizationStefan F. Martin

16:00-17:00Introduction and status reportof the Embryonic Stem cell-basedNovel Alternative Testing Strategies(ESNATS) EU project (EB11)Room: San PietroChair:Agapios SachinidisEmbryonic Stem cell-based NovelAlternativeTesting Strategies (ESNATS);progress report after 18 monthsAgapios SachinidisEmbryonic stem cells as tools for neurotoxicologyKarl-Heinz Krause

PLENARY SESSION 6 (PL6)17:25-18:10Room: AuditoriumChair: Umberto AgrimiIs forefront science consideredand applied in regulatory risk assessment?Vittorio Silano

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PLENARY SESSION 7 (PL7)09:00-09:45Room: AuditoriumChairpersons: Herman B.W.M. Koëter;Thomas HartungThe principles of humane experimental technique:timeless insights and unheeded warningsMichael BallsThe principles of humane experimental technique:is it relevant today?Alan M. Goldberg

Special Recognition Event09:45-10:15Room: Auditorium

Awards Ceremony10:15-10:45Room: AuditoriumChair: Coenraad Hendriksen• The Humane Society of the United Statesbestowing its 2009 Russell & Burch Award• Charles River Foundation bestowing itsexcellence in Enrichment Award• CAAT bestowing its recognition award• Doerenkamp-Zbinden Foundation bestowing itsyearly award for distinguished services to animalprotection in science• Doerenkamp-Zbinden Foundation bestowing 15poster awards• Bo Holmstedt Memorial Fund bestowing1 poster award•Young scientist participation awards• Intervet/Schering-Plough Animal Health bestowingits Dieter Lütticken Award to reduce the use ofanimals in research, development and manufacturingof veterinary medicine

PLENARY SESSION 8 (PL8)11:15-12:00Room: AuditoriumChair: Andrew RowanCalling on Science: making alternativesthe new toxicity-testing gold standardMelvin Andersen

WC8 Presentation12:00-12:15Room: AuditoriumClément J.T. Gauthier

Closing speeches12:15-12:45Room: AuditoriumThomas Hartung; Herman B.W.M. Koëter

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Poster exhibitionPoster exhibition

(PO1): INTEGRATED APPROACHESAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 7- A FRAMEWORK FOR USING STRUCTURAL, REACTIVITY,METABOLICAND SIMILARITYTO EVALUATETHE SUITABILITYOFANALOGS FOR SAR-BASEDASSESSMENTSS.Wu 1, J.Amburgey 2, K. Blackburn 1, J. Jaworska 3,T. Federle 11 Cincinnati, USA; 2 Mason, USA; 3 Brussels, BelgiumID ABS: 80 - BAYESIANASSESSMENT OF SKIN IRRITATION POTENTIAL,COMBININGTHE RESULTS FROM INDIVIDUAL PHYSICO-CHEMICALEXCLUSION RULESE. Rorije, J.P. Rila,T.AldenbergBilthoven,The NetherlandsID ABS: 81 - OPTIMIZATION OFAN INTELLIGENTTESTING STRATEGYTOASSESS FISHTOXICITY UNDERTHE REACH FRAMEWORKA.Roncaglioni 1,A. Lombardo 1,O. Schifanella 1,H. Segner 2, E. Benfenati 11 Milan, Italy; 2 Bern, SwitzerlandID ABS: 92 - APPLICATION OFTHETHRESHOLD OFTOXICOLOGICALCONCERN-CONCEPT IN SAFETYASSESSMENT OF CHEMICALLYCOMPLEX MIXTURESG. Houben, S. Koster, L. Krul, M. RennenZeist,The NetherlandsID ABS: 106 - COMPARATIVE ULTRASTRUCTURAL STUDY ONCHANGES OF PLATELETS AND FIBRIN NETWORKS IN HUMANANDMOUSEANIMAL MODELASTHMAR. Pretorius, N.OberholzerPretoria, South AfricaID ABS: 172 - STRATEGIES FORTHE RATIONAL INTEGRATION OFADME INFORMATION INTO PREDICTIONS FOR BIOLOGICALACTIVITYJ. Madden, F Bajot, M.T.D. CroninLiverpool, United KingdomID ABS: 539 - APPLICATION OFAN INTELLIGENTTESTING STRATEGYTOTHE US EPA ENDOCRINE DISRUPTOR SCREENING PROGRAMC.Willett 1, K. Sullivan 21 Norfolk, USA; 2Washington, DC, USAID ABS: 545 - INTEGRATEDTESTING STRATEGIES FORACUTEINHALATIONTOXICITYASSESSMENTK. Sullivan, C. Sandusky1Washington, USA

(PO2): CHEMICAL AND PHYSICAL METHODSAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 182 - POTENTIAL OFTHE DIRECT PEPTIDE REACTIVITYASSAY(DPRA) FORTHE IN CHEMICO DETECTION/DISCRIMINATION OFCHEMICAL RESPIRATORYVS SKIN SENSITIZERSJ.Lepoittevin 1,O.Fleischel 1,E.Gimenez-Arnau 1,L.Foertsch 2,F.Gerberick 21 Strasbourg, France; 2 Cincinnati, USAID ABS: 344 -VISCOSITY STUDIES OFWATER-SOLUBLE NONSTARCHPOLYSACCHARIDES EXTRACTED FROM FEEDSWITH DIFFERENTWHEAT PROPORTIONSR. Caprita, A. CapritaTimisoara, RomaniaID ABS: 345 - INFLUENCE OF EXTRACTION CONDITIONSANDBARLEY SOLUBLE BETA -GLUCANS CONTENT ONTHEWATEREXTRACTVISCOSITYA. Caprita, R. CapritaTimisoara, RomaniaID ABS: 421 - USE OF CHEMICAL REACTIVITYTO REFINETHE INVITROEYE IRRITATION PREDICTIONWITHTHE SKINETHIC-HCETMRECONSTRUCTED HUMAN CORNEAL EPITHELIUMC. Rousseau, D. Duché, J. Eilstein, G. Lereaux, E.Arbey,J.M.Ovigne, N.Alépée, J.R. MeunierAulnay sous Bois, FranceID ABS: 537 - CHEMICALALTERNATIVESTOTHEANIMALTESTSP. DiazMontevideo, Uruguay

(PO3): HIGH THROUGHPUT TECHNOLOGIESAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 29 - HTS FOR SELECTIVE MUSCARINIC CHOLINERGICRECEPTORSANTAGONISTSV.TonkopiiSt.Petesburg, RussiaID ABS: 76 - MINIATURIZEDTHREE-DIMENSIONAL CELL CULTUREANDMETABOLIC ENZYMEARRAYS FOR HIGH-THROUGHPUTTOXICITYASSAYSD. Rozzell 1, M.-Y. Lee 1, J. Ryan 1, J. Dordick 2, D. Clark 31 NY, USA; 2 Berkeley, CA, USAID ABS: 511 - ARTIFICIAL LIFE COULD INTHE FUTURETOREPLACEMENTTHEANIMALS FROMTHE LABORATORY?A. Moreno MorenoBogota, Colombia

(PO4): OMICS AND SYSTEMS BIOLOGYAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 12 - A MECHANISTIC COMPARISON OF INVITROAND INVIVO STUDIES OF LUNGTOXICITY USINGTRANSCRIPTOMICANALYSISA.White 1, K Donaldson 2, B Gaiser 2, P Daly 2, K Berube 3,T Hughes 3,L Merolla 1, P Carthew 1,Y Adeleye 1, S Fletcher 11 Bedford,United Kingdom;2 Edinburgh,United Kingdom;3 Cardiff,United KingdomID ABS: 145 - GENE EXPRESSION PROFILES AS ENDPOINTSIN HAZARD IDENTIFICATION OF ENVIRONMENTAL EXPOSUREP. Silingardi 1, E. Morandi 1, M.G. Mascolo 1, S. Perdichizzi 1, M.Vaccari 1,F. Rotondo 2,A. Guerrini 1, S. Grilli 1,A. Colacci 11 Bologna, Italy; 2 Reggio Emilia, ItalyID ABS: 274 - GENE ONTOLOGY CATEGORIZATIONANDBIOINFORMATIC PROCESSING IN HUMAN CELL CULTURE PHENOTYPINGR. Grafström 1, R. Ceder 1,2, M. Perala 11Turku, Finland; 2 Stockholm, SwedenID ABS: 352 - SYSTEMS BIOLOGY PBPK MODELLINGOF METABOLIC INTERACTIONSF. Bois1Verneuil en Halatte, FranceID ABS: 405 - TOXICOGENOMICS,ANOTHERTECHNOLOGICAL´HYPE´ ORATRUE REVOLUTION? HOWTO FURTHERTHE SOCIETALACCEPTANCEAND EMBEDDING OF EMERGINGTECHNOLOGIESM. Pijnappel1 Nijmegen,The NetherlandsID ABS: 479 - TOX-PROFILER;AWEB-APPLICATION FORINTERPRETATIONAND STORAGE OFTOXICOGENOMICS BASEDGENE-EXPRESSION DATAA. Boorsma 1, R.H. Stierum 21 Maastricht,The Netherlands; 2 Zeist,The NetherlandsID ABS: 480 - TRANSCRIPT PROFILING OFTHE INDUCTIONOF TERMINAL DIFFERENTIATION OF KERATINOCYTES AND ITSPOTENTIAL RELEVANCE INTOXICITYTESTINGR. Ceder 1,2, M. Merne 1, K. Roberg 3, R. Grafström 1,21Turku, Finland; 2 Stockholm, Sweden; 3 Linköping, SwedenID ABS: 494 - TOWARDSAN INVITRO SYSTEMS BIOLOGYAPPROACHTOASSESS (DEVELOPMENTAL) NEUROTOXICITYE.VanVliet 1, S. Morath 1, C. Eskes 1, P. Honegger 3, S. Coecke 2,T. Hartung 11 Baltimore, USA; 2 Ispra, Italy; 3 Lausanne, SwitzerlandID ABS: 531 - TRANSCRIPTOMICANALYSIS OFADIPOSETISSUEMETABOLICVARIATION IN HUMAN SUBJECTSA. Bennett, P.Tisdale, I. Macdonald, K.TsintzasNottingham, United KingdomID ABS: 541 -THE USE OF INVITROTOXICOGENOMICS FORSCREENING ESTROGENICACTIVITY: WHAT ISTHEADDEDVALUETOCLASSICAL ESTROGENICITY SCREENS?C.Vanparys,T.Hectors,K.Van derVen,M.Maras, J. Robbens,R. Blust,W.de CoenAntwerp, Belgium

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(PO5): NON-INVASIVE TECHNOLOGIESAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 110 - NON-INVASIVE ENDOCRINE MONITORING IN URINESAMPLES:A PREFERENTIAL METHOD FORASSESSING CHINCHILLAS(CH. LANIGERA) REPRODUCTIVE PHYSIOLOGYANDWELFAREM. Ponzio, J.M. Busso, R.D. Ruiz, M.H. Fiol de CuneoCórdoba,ArgentinaID ABS: 294 - LONGITUDINAL MEASUREMENTS OF BREATHINGFREQUENCY IN SEPHADEX CHALLENGED RATS. NON-INVASIVEMETHODTO MONITOR LUNG INFLAMMATIONH. Falk Håkansson, H. Siller, H. Lal, C. BrunmarkLund, SwedenID ABS: 336 - MEASUREMENT OF FECAL GLUCOCORTICOIDMETABOLITES AS ATOOLTO MONITOR STRESS DURING MATINGAND PREGNANCYM. Chelini 1, R. Palme 2, E. Otta 11 São Paulo, Brazil; 2Vienna,AustriaID ABS: 427 - INVIVO CONFOCAL MICROSCOPIC GRADING SYSTEMFOR NON-INVASIVE STANDARDIZED CORNEAL EVALUATION:APPLICATIONTOTOXIC-INDUCED DAMAGE IN RATA. Pauly, F. Brignole-Baudouin, H. Liang, J.M.Warnet, C. BaudouinParis, France

(PO6): NON-VERTEBRATE MODELSAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVEL

ID ABS: 470 - A NEWAPPLICATION OFTHE SLUG MUCOSALIRRITATION (SMI) ASSAY: DETECTING NASAL STINGING, ITCHINGAND BURNING (SIB)J. Lenoir, E.Adriaens, J.P. RemonGhent, Belgium

(PO7): IN SILICO MODELSAREA 2 - GALLERIA SAN PIETRO - LOBBY LEVELID ABS: 30 -VIRTUAL UTERUS:A COMPUTATIONALTOOL FORMYOMETRIUM FUNCTIONAND DEVELOPMENT DURING PREGNANCYW.Tong 1, P. Li 2, M.J.Taggart 3,A.V. Holden 11 Leeds, United Kingdom; 2 Fornebu, Norway; 3 Newcastle, United KingdomID ABS: 31 - USE OF CHEMOINFORMATICS IN PREDICTIVETOXICOLOGYP.Tiikkainen 1,A. Poso 2, O. Kallioniemi 1,3, R. Grafström 1,41Turku, Finland; 2 Kuopio, Finland; 3 Helsinki, Finland; 4 Stockholm, SwedenID ABS: 71 - CATEGORIZATION OF CHEMICALS FOR REPEATED DOSETOXICITYY. Sakuratani 1, S. Sato 1, S.Nishikawa 1, J.Yamada 1,A.Maekawa 1,M.Hayashi 1,21Tokyo, Japan; 2 Iwata, JapanID ABS: 87 - IN SILICO MODELSTO PREDICT RODENTCARCINOGENICITY OF NATURALLY-OCCURRING CHEMICALS:COMPARATIVE STUDYAND FIRST INSIGHTS INTO MODES OFACTIONP.Mazzatorta 2, S. Ringeissen 1, R. Note 1, B. Schilter 2, J.R. Meunier 11 Aulnay-Sous-Bois, France; 2 Lausanne, SwitzerlandID ABS: 88 - BUILDINGA DECISIONTREEALGORITHMTO PREDICTSKIN IRRITATION SEVERITY EFFECTSC.Yang 2, S. Ringeissen 1, R. Note 1, S. Loisel-Joubert 3, M.A. Lopez 1, C. Boulle1, J.M.Ovigne 1,A.Trotier-Faurion 1, G.Ouedraogo 1, J.R. Meunier 11 Aulnay-sous-Bois, France; 2 Columbus, USA; 3 Asnieres-sur-Seine, FranceID ABS: 90 - REDEFINING PHYSICO-CHEMICAL BOUNDARIES FORSKIN IRRITATIONAND CORROSION POTENTIALJ. Rila, E. Rorije,T.AldenbergBilthoven,The NetherlandsID ABS: 120 -SKIN SENSITISATIONANDAQUATICTOXICITYPREDICTION USING STRUCTURALALERTS AND OCTANOL-WATERPARTITION COEFFICIENTS INA KNOWLEDGE-BASED SYSTEMM. PayneLeeds, United Kingdom

ID ABS: 131 - KNOWLEDGE BASE OF BASICACTIVE STRUCTURES FROMTWENTY-EIGH