Calcium Cycling in Cardiac Cells Lin Guo-hua
Jan 29, 2016
Calcium Cycling in Cardiac Cells
Lin Guo-hua
Cardiac E-C coupling
Slow or L-type Ca2+ Channels (DHPRs)
L-type Ca2+ Channels
Ca2+ Release Channel of the Sarcoplasmic Reticulum (Ryanodine Receptor)
cardiac ryanodin receptor
Physiological and pathophysiological regulation
Ca2+ Pump of the Sarcoplasmic Reticulum
(SERCA)
SERCA
SERCA-PLN complex
Phospholamban (PLN)
&Sarcolipin
(SLN)
The Ca2+ Pump of the PlasmaMembrane (PMCA)
Sodium/Calcium Exchanger (NCX)
Troponin C (TnC)
Calmodulin (CaM)
Calmodulin (CaM) &
Calmodulin Kinase II (CaMKII)
Calmodulin Kinase II (CaMKII)
Regulation of Ca Transporters by CaM and CaMKII
frequency-dependent acceleration of relaxation (FDAR)
Calsequestrin (CSQ)
Organization of EC coupling elements
Integrative Analysis of Calcium Cycling in Cardiac Muscle
[Ca2+]i Transient in Heart Cell
[Ca2+]I Transient
[Ca2+]i sparks
&
Ca2+ Requirements for Activation of Myofilaments
Autoregulation of SR Ca2+ Content
Changes of sarcolemmal fluxes and SR CaCa2+ release during potentiation of CICR by caffeine (500 mmol/L)
Modulation of the RyR
relationships between the various Ca2+ fluxes
Ca2+ Influx During the Cardiac AP
Ca2+ Removal From the Cytosol During
Cardiac Relaxation
Frequency-dependent changes in the fraction of Ca release that is taken back up by the SR
Measurement of SR Ca2+ content and forcefrequency relationship.
force frequency relation
Frequency-dependence of isometric twitch force (twitch) and rapid cooling contractures (RCC) in nonfailing and failing human myocardium
Relationship between NCX and SERCA protein levels and in non-failing and failing human myocardium
Caffeine contractures in isolated rabbit myocytes following adenoviral gene transfer of LacZ and NCX
Frequency-dependent changes in twitch force, aequorin light signals RCCs in huma atrial muscles (A) and ventricular muscles (B).
Steady-state twitches, postrest twitches, and RCCs in isolated human atrial myocardium.
Rapid Cooling Contracture
Paired RCCs and Ca2+ Reuptake Into the SR
Rest-dependent changes in the amplitudes of twitch force and RCCs) in human atrial myocardium (A) and ventricular muscles (B)
postrest potentiation & postrest decay
restitution
postextrasystolic potentiation
Ca2+ Restitution of the Ca transient