Caenorhabditis Caenorhabditis elegans elegans (C. elegans) (C. elegans) Kathy Szeniawski Kathy Szeniawski Clayton State University Clayton State University Spring 2008 Spring 2008 Alternate Food Sources Alternate Food Sources Population Growth Population Growth & Behaviors & Behaviors
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Caenorhabditis elegans (C. elegans) Kathy Szeniawski Clayton State University Spring 2008
Caenorhabditis elegans (C. elegans) Kathy Szeniawski Clayton State University Spring 2008. Alternate Food Sources Population Growth & Behaviors. Non Parasitic “Bacteria Eating” Nematode 959 Somatic Cells Hermaphrodites and Males Egg/Embryonic Stage L1-L4 molts ? Dauer Stage (Hibernation) - PowerPoint PPT Presentation
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Worm ResearchWorm Research Research papers on “Different food source” Research papers on “Different food source”
experiments used different strains of experiments used different strains of bacteriabacteria to to feed to the worms. feed to the worms.
I hypothesized that wild type worms in their I hypothesized that wild type worms in their natural environment consumed food items since natural environment consumed food items since the lab worms that were collected were located in the lab worms that were collected were located in compost piles, mushroom beds and rotten fruit. compost piles, mushroom beds and rotten fruit.
N2 wild type (normal) worms usedN2 wild type (normal) worms used Transfer worms onto plates containing food Transfer worms onto plates containing food
productsproducts Observe and document population growth and Observe and document population growth and
Repeat experiment with controlled Repeat experiment with controlled seedingseeding
Use equal masses of food products Use equal masses of food products on all plateson all plates
Collect statistical data every day Collect statistical data every day – Population sizePopulation size– Amounts of Large worms vs. Small Amounts of Large worms vs. Small
wormsworms Observe behaviorObserve behavior
ConclusionConclusion
Different food sources CAN sustain Different food sources CAN sustain C. elegansC. elegans
Cannibalistic behavior observed several times; Cannibalistic behavior observed several times; Only larval worms observed participating in Only larval worms observed participating in cannibalismcannibalism
Larval worms observed killing/eating dust mitesLarval worms observed killing/eating dust mites Overall Population size quickly spikes, then Overall Population size quickly spikes, then
tapers off until only a few Dauer worms existtapers off until only a few Dauer worms exist Worm size in population tapers off to all small Worm size in population tapers off to all small
worms at the end of the growth curveworms at the end of the growth curve
Thank-YouThank-You
Dr. Francine Norflus, Research Dr. Francine Norflus, Research MentorMentor
Dr. Michelle FurlongDr. Michelle Furlong Larry and MaryEllen SzeniawskiLarry and MaryEllen Szeniawski Kheem TanKheem Tan Clayton State University Faculty and Clayton State University Faculty and
StaffStaff
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