BU M.Sc. Biochemistry Syllabus

DURGAPUR COLLEGE OF COMMERCE & SCIENCE

(DCCS)

RAJBANDH, DURGAPUR

PG DEPARTMENT

OF

THE UNIVERSITY OF BURDWAN

FOR

M.Sc. BIOCHEMISTRY

SYLLABUS

[According to the Choice Based Credit System (CBCS)]

FOR

M.Sc. BIOCHEMISTRY

THE UNIVERSITY OF BURDWAN

SYLLABUS

FOR

Program - M.Sc. BIOCHEMISTRY

SEMESTER - I

CODE COURSE DIVISION CORE /

ELECTIVE

L:T:P CREDIT

POINT

EXAM

HOURS

(Theory &

Practical )

FULL

MARKS

MBIOC

0101

BIOMOLECULES &

METABOLISM

THEORY &

PRACTICAL

CORE 4:0:4 8 2 & 5 100

MBIOC

0102

ENZYMOLOGY THEORY &

PRACTICAL

CORE 4:0:4 8 2 & 5 100

MBIOC

0103

PLANT

BIOCHEMISTRY

THEORY CORE 4:0:0 4 2 50

MBIOC

0104

NUTRITIONAL

BIOCHEMISTRY


Total Semester I marks = 300 Total Semester I Core Credit Points = 24

Total Semester I Credit Points = 24

SEMESTER - II


ELECTIVE

L:T:P CREDIT

POINT

EXAM

HOURS

(Theory &

Practical)

FULL

MARKS

MBIOC

0201

CHEMICAL

PRINCIPLES


MBIOC

0202

HUMAN

PHYSIOLOGY,

REPRODUCTIVE

BIOLOGY &

ENDOCRINOLOGY

THEORY

CORE

4:0:0 4 2 50

MBIOC

0203

MOLECULAR

BIOLOGY

THEORY &

PRACTICAL

CORE 4:0:4 8 2 & 5 100

MBIOC

0204

IMMUNOLOGY THEORY &

PRACTICAL

CORE 4:0:4 8 2 & 5 100

Total Semester II marks = 300 Total Semester II Core Credit Points = 24

Total Semester II Credit Points = 24

SEMESTER - III


ELECTIVE

L:T:P CREDIT

POINT

EXAM

HOURS

(Theory &

Practical)

FULL

MARKS

MBIOC

0301

MEDICAL

BIOCHEMISTRY

THEORY &

PRACTICAL

CORE 4:0:4 8 2 & 5 100

MBIOC

0302

BIOPHYSICAL &

BIOCHEMICAL

TECHNIQUES

THEORY &

PRACTICAL

CORE

4:0:2 6 2 & 3 75

MBIOC

0303

CELL BIOLOGY THEORY &

PRACTICAL

MAJOR

ELECTIVE

4:0:2 6 2 & 3 75

MBIOC

0304

GENETICS THEORY MAJOR

ELECTIVE

4:0:0 4 2 50

Total Semester III marks = 300 Total Semester III Core Credit Points = 14

Total Semester III Major Elective Credit Points=10

Total Semester III Credit Points = 24

SEMESTER - IV


ELECTIVE

L:T:P CREDIT

POINT

EXAM

HOURS

(Theory,

Practical &

Project

presentation)

FULL

MARKS

MBIOC

0401

PROJECT / TERM

PAPER / REVIEW

WORK

-

CORE

Notio

nal

hrs:

100

4 5 50

MBIOC

0402

BIOINFORMATICS,

BIOSTATISTICS &

RESEARCH

METHODOLOGY

THEORY &

PRACTICAL

CORE

4:0:2 6 2 & 3 75

MBIOC

0403

GENETIC

ENGINEERING

THEORY MAJOR

ELECTIVE

4:0:0 4 2 50

MBIOC

0404

MICROBIOLOGY THEORY &

PRACTICAL

MAJOR

ELECTIVE

4:0:4 8 2 & 5 100

Total Semester IV marks = 275+(*) Total Semester IV Core Credit Points = 10

Total Semester IV Major Elective Credit Points=12

Total Semester IV Minor Elective Credit

Points=(*)

Total Semester IV Credit Points = 22+(*)

* STUDENT WILL HAVE TO EARN ATLEAST 2 CREDITS (25 MARKS) FROM A MINOR

ELECTIVE THAT THEY WILL PURSUE FROM ANOTHER DEPARTMENT OF THE UNIVERSITY

OF BURDWAN DURING 4TH

SEMESTER.

MINOR ELECTIVE #


ELECTIVE

L:T:P CREDIT

POINT

EXAM

HOURS

(Theory)

FULL

MARKS

MBIOC

0405

GENERAL

BIOCHEMISTRY

THEORY MINOR

ELECTIVE

2:0:0 2 1 25

# MAY BE OPTED BY PG STUDENTS OF THE UNIVERSITY OF BURDWAN, EXCEPT THE

STUDENTS OF M.Sc. BIOCHEMISTRY. HOWEVER, THE NUMBER OF STUDENTS INTAKEFOR

MINOR ELECTIVE WILL BE SUBJECTED TO THE AVAILABLITY OF SEATS.

RULES & REGULATIONS OF CBCS (Choice Based Credit System):

Core course is the one which should compulsorily be studied by a candidate as a core requirement of

the Programme.

Major Elective course is the one that a candidate would choose from a pool of Major Elective

courses from the main discipline / subject of study.

Minor Elective course is the one that a candidate would choose from a pool of Minor Elective

courses from other Departments of The University of Burdwan.

A candidate has to register for a minimum of 20 credits per semester and maximum being 24 credits

per semester.

A candidate has to earn a minimum of 82 credits for successful completion of a Master’s degree with

a distribution of credits for different courses as following:

* This crediting is exclusively for the Department of Biochemistry

A candidate can earn a maximum of 96 credits during completion of Master’s degree.

COURSE TYPE CREDIT*

Core 72 Credits

Major Elective Minimum of 16 Credits and

Maximum of 22 Credits

Minor Elective Minimum of 02 Credits

MBIOC0101

BIOMOLECULES & METABOLISM

CREDIT: 08 FULL MARKS: 100

Theory:

1. Carbohydrate: Classification; Conformational analysis of monosaccharides and disaccharides

(pentoses and hexoses); anomeric effect, reverse anomeric effect and their origin; mutarotation and

abnormal mutarotation; a detailed account regarding the structures of polysaccharides; isolation,

purification, properties; Reducing and non-reducing sugar.

2. Amino acids & Proteins : classification of amino ac ids; non standard amino acids, non-protein

functions of amino acids; dipolar ion, isoelectric point; ninhydrin reaction; synthesis and properties

of essential amino acids; peptide bond ;Proteins Classification ; Denaturation; primary structure, C-

terminal and N-terminal amino acid determinations; secondary, and quaternary structures,

Ramachandran plot, helix-coil transition, factors responsible for stabilizing interaction of secondary

and tertiary structures, protein folding; determination of amino acid sequences in proteins; study of

myoglobin and hemoglobin and collagen; Merifield’s solid-state peptide synthesis.

3. Nucleic acids : Classification, occurrence, nucleoside, nucleotides - structures of ribosyl and

deoxyribosyl nucleotides, functions, Detailed account of stabilizing interactions in DNA structure;

determination of nucleotide sequence in DNA, structural polymorphism of DNA and RNA,

secondary and tertiary structure of tRNA; keto-enol tautomerism and hyperchromism.; a short

account on miRNA, siRNA, snRNA.

4. Bioenergetics: The laws of thermodynamics, concept of entropy and free energy; bioenergetics of

ATP synthesis and hydrolysis, high energy bonds in biomolecules.

5. Carbohydrate metabolism, Glycolysis: Embden Meyerhoff pathway; glyoxylate cycle; glycogen

biosynthesis, glycogenolysis; gluconeogenesis; pentose phosphate regulation.

6. Amino acid metabolism, essential amino acid biosynthesis : Histidine, tryptophan, tyrosine,

aspartic acid, methionine, regulation, concerted and sequential feed back; degradation of amino acid

with reference to ketogenic, glucogenic and both types of amino acids, urea cycle; biosynthesis of

heme, glutathione, polyamines and their significances.

7. Fats, Lipids & Lipid metabolism: Classification, lipids in biological membrane; membrane

fluidity, lipid-linked proteins, detailed structure and a brief account on functions of lipids.

Biosynthesis and catabolism of lipids, lipoproteins; regulation of lipid metabolism; lipid transport.

8. Nucleotide biosynthesis: de- novo and salvage pathway and their regulation, abnormal synthesis

and diseases.

Practical:

1. Separation of mixture of sugars by thin-layer chromatography, leaf pigments by column

chromatography and amino acids by paper chromatography.

2. Isolation of casein from milk sample.

3. Estimation of amino acid by ninhydrin method

4. Determination of N- terminal and C- terminal amino acids of a known protein

5. Estimation of protein content by Biuret and Lowry & Bradford’s method.

6. Estimation of reducing sugar content by DNS method and total sugar by Anthrone method

7. Extraction and estimation of ascorbic acid

8. Estimation of DNA by DPA method

9. Estimation of RNA by orcinol method

10. Determination of lipid composition of wheat grain.

11. Determination of acid value and saponification value of natural fats.

Suggested reading:

Principle of Biochemistry- D.L.Nelson and M.M.Cox, Freeman and Co.

Biochemistry- J.M.Berg, J.L.Tymocko and L.Stryer, Freeman and Co.

Biochemistry- D.Voet and J.G.Voet, John Willey and Sons

Principles of Biochemistry- H.R.Horton, L.A.Moran and G.Scrimgeour, D.Perry, D.Rawn, Prent Hall

Out lines of Biochemistry- E.E.Conn, P.K.Stumph, G.Bruening and R.H.Doi, John Willey and Sons

Instant notes of Biochemistry- B.D.Hames and N.M.Hooper, Viva Books

Thermodynamics for Chemists- S.Glasstone, Affiliated East-West Press

An Introduction to Thermodyanamics- Y.V.Rao, Universities Press

Harper’s Illustrated Biochemistry- R.K.Murray, D.K.Grannes and V.W.Rodwell, McGraw Hill

Organic Chemistry – R. T. Morrison & R. N. Boyd, Prentice Hall

Organic Chemistry vol. 1&2 – I.L. Finar, Pearson Edition.

Stereochemistry of Carbon Compounds- D.Nasipuri, New Age International

An Introduction to Practical Biochemistry – David T. Plummer, Tata – McGraw Hill.

Biochemical Methods – Sadasivam and Manikam, New Age Pub.

Note:

Mid semester tests shall be conducted during the 8th

and 16th

weeks of the semester period on the

topics of this paper. The marks obtained will be considered for the final awarding.

Continuous Assessment: [C1= 10 marks, C2= 10 marks & C3= 80 marks (Theory: 40 marks &

Practical: 40 marks)].

Marks distribution (Theory): 02 questions of 08 marks (out of 04), 04 questions of 04 marks (out of

08) & 08 questions of 01 mark (out of 12).

Marks distribution (Practical): 01 question of 18 marks (compulsory), 01 question of 10 marks (out

of 02), Practical note book – 04 marks & Viva-voce – 08 marks.

MBIOC0102

ENZYMOLOGY


Theory:

1. Basic Concepts: Nomenclature and classification, special characteristics of enzymes, cofactors,

coenzymes, ribozyme.

2. Enzymatic Catalysis & Kinetics: Concept of free energy for understanding enzymes, investigation of

structure and common features of active site, enzyme specificity, binding energy, rate constant, order and

molecularity of reactions, factors affecting rate of chemical reactions, activation energy, kinetics of

uncatalyzed reaction, steady state concept, Michaelis-Menten model, significance of KM & Vmax values,

kinetic perfection in enzymatic catalysis, kinetics of multisubstrate reactions.

3. Catalytic Strategies: Basic catalytic principles, proteases – mechanism of action of peptide hydrolysis by

chymotrypsin, its catalytic triad, active sites analysis of cysteine, aspartyl and metalloprotease, mechanism

of actions of carbonic anhydrase, restriction endonuclease and nucleotide monophosphate kinases.

4. Enzyme Inhibition: Types of inhibition, kinetics of competitive and noncompetitive inhibitors,

mechanism based inhibition and pharmaceutical importance, transition state analogs and catalytic

antibodies, protease inhibitors, mechanism of penicillin activity.

5. Regulatory Strategies: Allosteric control, multiple forms of enzymes, reversible covalent modification,

proteolytic activation. Serine & threonine kinases and their activating signals, regulations of protein kinase

A, glycogen phosphorylase and pyruvate dehydrogenase complex.

6. Models for Enzyme Regulation: Cooperativity of oligomeric enzymes and Hill equation; the Adair

equation and MWC model, KNF model; regulation of aspartate transcarbamylase activity; mnemonical

enzymes.

7. Applications: Clinical and diagnostic importance of different isozymes, enzyme immobilization and

industrial implications of immobilized enzymes; uses of type II restriction endonucleases; uses of enzymes

in biosensors.

Practical:

1. Subcellular fractionation of different intracellular organelles by differential centrifugation and

confirmation of the fractions using marker enzymes assay.

2. Assay of salivary -amylase by DNS method and germinating seed -amylase by starch-iodine

assay.

3. Purification of alkaline phosphatase from plant sources.

4. Biochemical characteristics of purified alkaline phosphatase: i) progress curve analysis ii) pH and

temperature optima iii) effect of Mg++

ion and F- ion and iv) Km and Vmax determination.

5. Assay of Serum lactate dehydrogenase activity, effect of inhibitors on LDH activity and

determination of inhibitor constant (ki).

6. Separation of isoenzymes of LDH by polyacrylamide gel electrophoresis.

Suggested reading: Enzymology-Biochemistry,Biotechnology,Clinical Chemistry- T. Palmer, Affiliated East-West Pres




An Introduction to Practical Biochemistry – David T. Plummer, Tata – McGraw Hill.

Note:

Mid semester tests shall be conducted during the 8

th and 16

th weeks of the semester period on the








MBIOC0103

PLANT BIOCHEMISTRY


Theory:

1. Cytosolic Carbon Metabolism : Anabolism and catabolism, carbohydrate synthesis and

degradation–central role of hexose phosphates; sucrose metabolism and its importance, synthesis and

breakdown; starch synthesis and breakdown, glycolysis and its enzymes, pentose phosphate pathway

and its enzymes; anaerobic respiration; entry of hexose phosphates into glycolysis.

2. Mitochondrial Metabolism : Carbon metabolism in mitochondrion- enzymes and reactions of TCA

cycle, catabolic and anabolic role of TCA cycle – removal of TCA cycle intermediates for anabolic

purpose and their replenishment through anaplerotic reactions; oxidation of mitochondrial NADH

and synthesis of ATP (oxidative phosphorylation), cyanide resistant pathway (alternative oxidase

pathway).

3. Photosynthetic carbon metabolism : Role of light - PS-I and PS-II reactions, formation of ATP

(Photophosphorylation), reducing power characteristics of ribulose-1, 5-bisphosphate carboxylase-

oxygenase (RUBISCO) - activation, mechanism and regulation, Calvin cycle and C2 cycle

(glycolate metabolism), carbon metabolism through C4 and CAM pathways, CO2 concentrating

mechanism; chloroplast - cytosol interactions - transport of proteins into chloroplast; flux of carbon

between chloroplast and cytosol.

4. Nitrogen metabolism: Molecular biology of N metabolism, N2 fixation, NO3 reduction and NH4

assimilation; outlines of biosynthesis of an aliphatic and an aromatic amino acid; protein

biosynthesis and protein turnover.

5. Plant Hormones: Outlines of biosynthesis of IAA, GA, zeatin, ethylene and ABA and their major

developmental role (cell division and growth, seed germination, dormancy, flowering (senescence

and fruit ripening).

Suggested reading:

Plant Biochemistry- Hans, Walter, Heldt, Elsevier pub

Biochemistry and Molecular Biology of Plants- B.Buchanan, W.Gruissen and L.Johnes, IK Int

Molecular Plant Biology – P.M.Gilmartin and C.Bowler, Oxford University Press

Plant Physiology- W.G.Hopkins and P.AHuner, John Willey and Sons

Plant Physiology- L.Teiz and E.Zeiger, Panima pub.




Principles of Biochemistry- Horton, Moran, Scrimgeour, Perry and Rawn, Prentice Hall

Note: Mid semester tests shall be conducted during the 8

th and 16



Continuous Assessment: [C1= 05 marks, C2= 05 marks & C3= 40 marks (Theory: 40 marks)].



MBIOC0104

NUTRITIONAL BIOCHEMISTRY


Theory:

1. Basic concepts: Energy content and thermogenic effect of foods; measurement of energy expenditure;

direct and indirect calorimetry; definition of BMR and SDA and their affecting factors.

2. Carbohydrates: Dietary requirements and sources of natural carbohydrates; physiological action of

carbohydrates (dietary fibre).

3. Proteins: Protein reserves of human body; nitrogen balance studies and factors influencing nitrogen

balance; essential amino acids and concepts of protein quality; cereal proteins and their limiting amino

acids; protein requirement at different stages of development.

4. Lipids: Major class of dietary lipids; properties and composition of plasma lipoproteins and

conjugated lipids; dietary needs of lipids; essential fatty acids and their physiological functions.

5. Electrolytes and water balance: Electrolyte concentrations of body fluids; concept of acidosis and

alkalosis.

6. Minerals: Nutritional significance of dietary calcium, phosphorus, magnesium, iron, iodine, zinc and

copper; trace elements (selenium, cobalt and molybdenum) nutrition.

7. Vitamin: Dietary sources, biochemical functions and specific deficiency diseases.

8. Nutritional requirements: Nutritional requirements during pregnancy and lactation of infants and

children.

Suggested reading:

Nutritional Biochemistry- Tom Brody

A Text Book of Medical Biochemistry- M.N.Chatterjea and R.Shindea, Jaypee pub.


Lippincott’s Ellastrated Biochemistry- P.C.Champe, R.A.Hervey and D.R.Ferrier, Wolters Kluwer

Medical Physiology- A.C.Guyton and J. E. Hall, Saunders pub

Human Physiology- C. C. Chatterjee, Medical Allied Agency

Nutritional Biochemistry- Swaminathan

Note:


and 16th






MBIOC0201

CHEMICAL PRINCIPLES


Theory:

1. Atomic structure and periodic properties : Introduction to Quantum Mechanics: wave particle

duality, uncertainty principle, standing waves, stationary states, atomic orbitals, Postulates of

Quantum Mechanics, Particle in a box, quantum mechanical model of hydrogen like species; Born

Oppenheimer approximation, LCAOMO method.

2. Periodic Table, Structure & Bonding: Modern form of periodic table, periodic properties;

ionization energy, election affinity, electronegativity, atomic / ionic size, ionic potential. Ionic and

covalent bonding, ionic solids, ionic radii, lattice energy (Born-Haber Cycle); M.O. and V.B.

approaches for diatomic molecules, VSEPR theory and shapes of molecules, hybridizations,

resonance, dipole moment, structure parameters such as bond length, bond angle and bond energy,

hydrogen bonding, Van der Waals interactions.

3. s-, p-, and d- Block Elements : Important bio-inorganic compounds of representative elements of

alkali and alkaline earth metals, coordination complexes of transition elements; valence bond and

crystal field theory, color, geometry and magnetic properties, applications of coordination

molecules in different fields of chemistry and related disciplines.

4. Chemical Equilibria : Colligative properties of solutions, ionic equilibria in solution, solubility

product, common ion effect, hydrolysis of salts, pH, buffer and their applications in chemical

analysis, equilibrium constants (Kc, Kp and Kx) for homogeneous reactions.

5. Electrochemistry: Conductance, Kohlrausch law, half cell potentials, emf, Nernst equation,

galvanic cells, thermodynamic aspects and their applications.

6. Reactive Intermediates and Organic Reactions Mechanisms : Classical and non-classical

carbocations and carbanions, radicals, carbenes, arynes, nitrenes, -generation, stability, structure

and reactions; SN1, SN2, E1 and E2 reactions, Hoffmann and Saytzeff rules, addition reactions,

Markonikoff rule and Kharasch effect, Diels-Alder reaction, aromatic electrophilic substitution,

orientation effect as exemplified by various functional groups, identification of functional groups

by chemical tests, rearrangements.

7. Structure-Reactivity Correlations: Acids and bases, electronic and steric effects, optical and

geometrical isomerism, tautomerism, conformers, concept of aromaticity, thermodynamic and

kinetic parameters, correlation diagram, Hammett equation, Taft equation.

Suggested reading:

Concise Inorganic Chemistry- J.D.Lee, Blackwell Science

Advanced Inorganic Chemistry- Cotton

Advanced Organic Chemistry-Reactions, mechanism and structure- Jerry March, John Wiley & Sons

Organic Chemistry- R.T.Morrison and R.N.Boyd, Prentice Hall

Organic Chemistry- T.W.G.Solomons and C.B.Fryhle, John Wiley & Sons

Physical Chemistry- P.C.Rakshit, Sarat Books Distributors

Atkins’ Physical Chemistry- P. Atkins and J.D.Paula, Oxford University pub

Elementary Physical Chemistry- S.R.Palit, Book Syndicate pub

Instants Notes in Chemistry for Biologist- J.Fisher and J.R.P Arnold, Viva pub

A Guide Book to Mechanism in Organic Chemistry- Peter Sykes, Pearsons pub

Notes:


th and 16






MBIOC0202

HUMAN PHYSIOLOGY, REPRODUCTIVE BIOLOGY & ENDOCRINOLOGY


Theory:

1. Digestive system: Basic structure and organization of digestive system; digestion, absorption and

assimilation in the gastrointestinal tract.

2. Respiratory system: Structure and function of trachea and lung; transport of O2 and CO2; lung

surfactants.

3. Cardiovascular system: Heart as a pump; lymphatic system; blood composition and function; cardiac

cycle.

4. Muscle: Types and structure; mechanism of muscle contraction.

5. Nervous system : Organization of nervous system; development of action potential and nerve impulse,

neurotransmitters and its action; major sense organ and receptors.

6. Excretory system: Nephron; mechanism of urine formation (filtration); osmoregulation - acid-base

balance and its regulation; composition of urine.

7. Reproductive Biology: Gametogenesis - molecular events during fertilization, mechanisms of

ovulation, fertilization and implantation; control of ovulation – role of hormones and drugs; activators and

inhibitors of sperm function.

8. Endocrine system: Hormones of anterior pituitary, thyroid and pancreas – chemical structure of

hormones and physiological roles; biosynthesis of thyroxine and sex steroids; role of cAMP.

Suggested reading:

Medical Physiology- A.C.Guyton,

Medical Physiology- W.F.Ganong, McGraw Hill

Principles of Anatomy and Physiology- G.J.Tortora, B.Derickson, John Wiley and Sons pub

Human Physiology- Dr.C.C. Chatterjee, Medical Allied Agency

Notes:


and 16th






MBIOC0203

MOLECULAR BIOLOGY


Theory:

1. Introduction: Abiotic origin of life, RNA world, central dogma of molecular biology, universality of

DNA as the genetic material except in plant virus and retrovirus.

2. Nucleic acid: Structure of DNA, A, B and Z DNA; structure of RNA and their function; chromatin

structure, super helical DNA, enzymes changing the super helicity.

3. Replication: Semiconservative replication, Messelson and Stahl experiment, bidirectional replication,

enzymes involved in prokaryotic and eukaryotic replication, replication process in prokaryotes, replication

inhibition.

4. Transcription: Transcription in prokaryotes and eukaryotes, promoters, enhancer and transcription

factors, transcription inhibition.

5. Post transcriptional modifications: Processing of m RNA - splicing, 5’ capping and polyadenylation,

rRNA and tRNA processing in prokaryotes and eukaryotes.

6. Translation: Genetic code – nature and properties of genetic code, translation in prokaryotes and

eukaryotes, factors involved, post translational modifications, signal sequences, inhibition by antibiotic.

7. Gene expression: Operon - lactose, tryptophan and arabinose, catabolite repression, λ phage gene

regulation, environmental regulation of gene expression.

8. Recombination: Homologous and site specific recombination, transposable elements in prokaryotes and

eukaryotes, Retroposons.

9. Oncology: Oncogenesis, mode of action of different carcinogens, role of retrovirus, P53

gene and other

tumor suppressor genes, protooncogenes, apoptosis.

Practical:

1. Isolation of chromosomal DNA from E.coli and plants.

2. Characterization of isolated DNA by Agarose gel Electrophoresis.

3. Isolation of plasmid DNA from E.coli.

4. Transformation of E.coli with plasmid.

5. Cloning of prokaryotic DNA using a suitable vector

6. Restriction digestion of plasmid DNA with E.coli.

7. DNA amplification using PCR method

8. UV induced mutagenesis in E. coli

9. SDS-PAGE and determination of molecular weight of unknown protein.

Suggested reading:

Molecular and Cell Biology-Alberts, Johnson, Lewis, Raff, Roberts and Walter, Garland Sc

Molecular Cell Biology- Lodish, Berk, Matsudaira, Scott, Zipursky and Darnell, Freeman pub

Principles of Genetics- D.P.Snustad and M.J.Simmons, John Willey and Sons

i- Genetics- P.J.Russel, Pearson-Benjamin Cumming

Cell and Molecular Biology- Gerald Karp, John Willey and Sons

Essential of Molecular Biology- D.Friefelder, Narosa pub

Advanced Molecular Biology-Aconcise reference- R.M.Twyman, Viva

The Cell- A Molecular Approach, G.M.Cooper, R.E.Hausman, ASM Press

Molecular Plant Biology- P.M. Gilmartin and C.Bowler, Oxford University

Freifelder’s Molecular Biology- G.M.Malacinski, Narosa

Biochemistry and Molecular Biology- W.H.Elliot and Elliot, D.C.Elliot, Oxford University pub

Genome- T.A. Brown, Garland Science

Molecular Biology of the Gene- Watson, Baker, Bell, Gann, Levine, Losick, Pearson Education

Genes VIII- B.Lewin, Prentice Hall

Principles of Biochemistry- D.L.Nelson and M.M.Cox, Freeman and Co.

Note:


th and 16









MBIOC0204

IMMUNOLOGY


Theory:

1. Immunity and immune response : Cells and organs of the immune system (including thymus), T

cell, B cell and macrophages, innate and acquired immunity; primary and secondary immune response;

humoral and cell mediated immunity; antigen - immunogenicity, haptens and antigenecity, epitopes –

properties.

2. Antibody / Immunoglobulins: Structure and function of different types of immunoglobulins;

immunoglobulin genes, generation of antibody diversity; affinity maturation.

3. Antigen recognition and Lymphocyte activation: T-cell, different types and functions; T-cell

receptor; T-cell maturation; B cell activation and maturation; MHC- different types, antigen processing

and presentation, complement system; grafting - autograft and allograft; autoimmunity.

4. Effector mechanisms of Immune Responses: Cytokines and its action.

5. Immunotechniques: ELISA, RIA, FACS, western blotting, immunofluorescence, immunodiffusion,

immunoelectrophoresis; hybridoma related to monoclonal antibody production.

Practical

1. Immunization of animal with any antigen, isolation and purification of the antibody.

2. Ag – Ab reactions – agglutination.

3. Quantitative precipitin test.

4. Ouchterlony Immunodiffusion test.

5. Immunoelectrophoresis

6. Albumin determination by Laurell rocket immunoelectrophoresis.

7. Antigen detection by Dot ELISA method.

8. Agglutination inhibition test.

Suggested reading: Cellular and Molecular Immunology- A.K.Abbas and A.H.Lichtman, Saunders pub

Immunology- R.A.Goldsby, T.J.Kindt, B.A.Osborne, J.Kuby, W.H.Freeman and Co.

Immunology- I.Roitt, J.Brostoff, D.Male, Mosby pub

Instants Notes Immunology- P.M.Ladyard, A.Whelam and M.W.Fanger, Viva Books pub

Note:


th and 16





Marks distribution (Theory): 02 question of 08 marks (out of 04), 04 questions of 04 marks (out of




MBIOC0301

MEDICAL BIOCHEMISTRY


Theory:

1. Disorders of carbohydrate metabolism: Hypo- and hyper-glycemia, various types of glucose

tolerance tests; galactosemia.

2. Lipids, Lipoproteins and Apolipoproteins: Physiology of lipoproteins, lipidosis in detail;

clinical inter-relationships of lipids like sphingolipidosis and multiplesclerosis; atherosclerosis;

Hyperlipoproteinemia; Familiail hypercholesterolemia.

3. Diagnostic Enzymes: Principles of diagnostic enzymology; clinical significance of creatinine

kinase, lactate dehydrogenase, SGPT and SGOT.

4. Disorders of Hormones and Minerals metabolism: Diseases of the Thyroid gland; steroid

hormone- progesterone and progestrogen; calcium and phosphorous; osteoporosis;

Hypogonadism.

5. Biochemical Aspects of Hematology: Haemoglobinopathies, thalassemias and anemias; blood

clotting, homeostasis and thrombosis, extrinsic and intrinsic pathways of blood clotting.

6. Detoxification mechanism in the body: Enzymes of detoxification, detoxification through

cytochrome P450.

Practical:

1. Estimation of blood glucose, detection of serum- (i) urea, (ii) uric acid, (iii) creatine, (iv) creatinine

and (v) bile pigment

2. Blood grouping (ABO – Rh)

3. Determination of urine sample for the presence of (i) sugar, (ii) ketone bodies, (iii) protein, (iv) bile

pigment

4. Estimation of Hb concentration, total W.B.C and R.B.C count in blood

5. Determination of lipid, total cholesterol, LDL and HDL, and triglycerides

6. Quantitative estimation of blood alkaline phosphatase and acid phosphatase.

7. Social outreach Programme (through Field work): Visit to nearby villages & perform

(Awareness Camp / Health Camp / Advisory Camp / etc.)*

* Programme detail will be as per the decision of the Department.

Suggested reading:

A Text Book of Medical Biochemistry- M.N.Chatterjea and R.Shindea, Jaypee pub. Harper’s Illustrated Biochemistry- R.K.Murray, D.K.Grannes and V.W.Rodwell, McGraw Hill

Lippincott’s Ellastrated Biochemistry- P.C.Champe, R.A.Hervey and D.R.Ferrier, Wolters Kluwerl

Text Book of Medical Physiology- A.C.Guyton, J.E.Hall, Saunders pub

Medical Physiology- W.F.Ganong, McGraw Hill pub

Marks Basic Medical Biochemistry- M.A.Liberman and Allan Marks,

Clinical Chemistry and in Diagnosis and Treatment- P.D.Mayne

Text Book of Biochemistry- D.M.Vasudevan and Seekumari, S, Jaypee pub.

Note:


and 16th





Marks distribution (Theory): 02 question of 08 marks (out of 04), 04 questions of 04 marks (out of



of 02), Practical note book – 04 marks & Viva-voce – 06 marks, Field work – 05 marks, Field report

– 05 marks & Viva-voce (on field work) – 05 marks.

MBIOC0302

BIOPHYSICAL & BIOCHEMICAL TECHNIQUES


Theory:

1. Ultraviolet Spectroscopy: Principle, elucidation of stereochemistry, effects of solvents, applications in

aromatic, heterocyclic and biological molecules, Fluorescence and Phosphorescence, Fluorescence

Spectroscopy.

2. Infrared Spectroscopy: Concept of vibrational spectra, Identification of functional groups, simulation of

bio-reactions, FTIR and its advantages.

3. Nuclear Magnetic Resonance Spectroscopy: Nuclear spin, NMR – active nuclei, principle, chemically

equivalent and nonequivalent protons, chemical shifts and coupling constants, characteristics of 1H-NMR

signals in simple biomolecules.

4. Mass Spectrometry: Electron impact mass spectroscopy, low and high resolutions, molecular ion,

fragment ions (odd / even electron type), factors affecting cleavage pattern, biological applications; MALDI-

TOF and its application in analysing proteins and other macromolecules.

5. Radiation and Tracer technique & Liquid Scintillation Spectrometry : Introduction, principle,

methodology and biological application : Concept and methodology; tracer packet and its developments,

application in interpretation of biochemical process; tracer technique in biology, radiation dosimetry,

radiation dosimetry, radioactive isotope and half life of isotope, effect of isotope in biological system;

autoradiography, Cerenkov radiation.

6. Chromatography, Electrophoresis and Centrifugation : Introduction, principle and biological

applications of column chromatography, thin-layer chromatography, paper chromatography, high

performance liquid chromatography, ion exchange chromatography, affinity chromatography, gas liquid

chromatography and molecular exclusion chromatography; ultracentrifugation.

Practical:

1. Verification of Lambert’s – Beer’s Laws by spectrophotometric method

2. Absorption spectra of Hb isolated from human blood.

3. Spectrophotometric analysis of DNA and RNA

4. Colorimetric determination of pKIN

5. Determination of viscosity of DNA solution.

6. Determination of heat of activation of α-amylase.

Suggested reading:

Biophysical Chemistry- A.Upadhaya, K.Upadhaya and N.Nath, Himalaya Publishing House

Physical Biochemistry- Applications to Biochemistry and Molecular Biology- D. Freifelder, Freeman

Biophysical Chemistry- Debojyoti Das, Academic pub

Biophysical Chemistry- R.N.Roy

Practical Biochemistry-Principles and techniques- K. Wilson and J.Walker, Cambridge pub

Spectroscopy of Organic Compounds- P.S. Kalsi

Organic Spectroscopy- Willium Kemp

Elementary Organic Compounds- Y.R.Sharma

Application of Absorption Spectroscopy of Organic Compounds- J.R.Dyre, Prentice Hall

Note:


th and 16



Continuous Assessment: [C1= 7.5 marks, C2= 7.5 marks & C3= 60 marks (Theory: 40 marks &






MBIOC0303

CELL BIOLOGY


Theory:

1. Cellular Boundaries: Electon microscopic structure of cell wall (prokaryotes & eukaryotes):

function: Cell Membrane; membrane architecture, asymmetry, dynamics of membrane fluidity,

proteins in membrane, transport mechanism through membrane (Active & passive).

2. Cellular organelles: GERL system, Architecture, enzymes & function; hydroxylation system

(oxidase system and cytochrome P-450) in ER, glycosylation, role in tissue dynamics (fertilization

and germination of seed) of Lysosome; diseases.

3. Cell Division & Growth: Over view of cell cycle & its regulation, Mitosis & Meiosis at Molecular

level.

4. Protein sorting / targeting: Intracellular protein transport to Mitochondria, Chloroplast,

Peroxisome, Lysosome, Endoplasmic reticulum, Golgi body & plasma membrane.

5. Mitochondria: Ultrastructure; organization of ETC, ATP synthetase, mechanism of oxidative

phosphorylation.

6. Chloroplast: Ultrastructure; pathways of photophosphorylation, mechanism of ATP formation, C3

and C4 pathway.

7. Nucleus: Ultrastructure, envelope - ultrastructure, pore complex, protein transport via pore complex,

structure and organization of chromatin.

8. Cytoskeleton: Structural organization of microtubules, intermediate filaments and microfilaments.

9. Cell Signaling : Molecular basis of cell signaling, G-protein coupled receptors, second messenger,

IP3 and DAG pathway, RTK system, JAK-STAT.

Practical:

1. Study of Mitosis & Meiosis in plant material, following acetocarmine & acetoorcein squash

technique.

2. Study of Karyotype in root tips of Allieum sepa & Allieum sativa following acetoorcein

squash technique.

3. Demonstration of spiral nature of chromosome.

4. Demonstration of orcein breakage of chromosome.

5. Determination of chiasma frequency.

6. Study of effect ionizing radiation on chromosomes.

Suggested reading:

Molecular Cell Biology- Lodish, Berk, Matsudaira, Scott, Zipursky and Darnell, Freeman pub

Cell and Molecular Biology- Gerald Karp, John Willey and Sons The Cell- A Molecular Approach, G.M.Cooper, R.E.Hausman, ASM Press

Essential Cell Biology- Alberts, Bray, Hopkin, Johnson, Lewis, Raff, Walter, Garland Science

Cell and Molecular Biology- E.D.P.DeRobertis and E.M.F.DeRobertis, Williums & Wilkins

Molecular Biology of the Cell- Alberts, Johnson, Lewis, Raff, Roberts and Walter, Garland Sc

Note:


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MBIOC0304

GENETICS


Theory:

1. Mendelism: Chromosomal basis of inheritance – Mendelian concept, multiple alleles, gene interaction,

linkage.

2. Non Mendelian inheritance: Cytoplasmic inheritance and maternal effects (snail coiling and kappa

particle inheritance), cytoplasmic male sterility.

3. Sex linked inheritance: X-linked, Y-linked and XY- linked inheritance.

4. Sex determination: Genetic basis of sex determination and dosage compensation in Drosophila, C.

elegans and human.

5. Gene and its function: Modern concept, cistron, recon and muton; inborn error of metabolism;

complementation.

6. Gene Mapping: Bacteria, Neurospora, Drosophila, pedigree analysis in human.

7. Chromosomal anomalies: Structural and numerical changes in chromosome.

8. Mutation: Types; methods for detection (ClB and Muller- 5); molecular basis of mutation; DNA damage

and repair, P elements in Drosophila sp.

9. Human cytogenetic : Karyotype and nomenclature; chromosomal banding; genetic basis of diseases like

down syndrome, Klienfelters syndrome, Turner’s syndrome, cry-du-chat syndrome, chronic myelogeneous

leukemia, burkitts lymphoma and retinoblastoma.

10. Population Genetics: Hardy - Weinberg theory; factors affecting Hardy- Weinberg equilibrium.

11. Genomics: Present status – functional and structural genomics; concept of physical, cytological and

genetic map; chromosome walking; chromosome jumping; brief outline of human genome project.

Suggested reading:

Principles of Genetics- D.P.Snustad and M.J.Simmons, John Willey and Sons

i- Genetics, P.J.Russel- Pearson-Benjamin Cumming

Genetics- M.W.Strickberger, Prentice Hall

Concepts of Genetics- W.S.Klug and M.R.Cummins, Pearson pub

Principles of Genetics- R.H.Tamarine, Tata McGraw Hill

Principles of Genetics- E.W.Sinnot, L.C.Dunn, T.Dobzhansky, Tata McGrawl Hill pub

Cell and Molecular Biology- E.D.P.DeRobertis and E.M.F.DeRobertis, Williums & Wilkins

Instants Notes in Genetics- P.C.Winter, G.I.Hickey and M.L.Fletcher, Viva Books

Note:


th and 16






MBIOC0401

PROJECT / TERM PAPER / REVIEW WORK


The student has to spend 100 notional hours on Project work / Term Paper / Review

work.

Marks distribution: Project work / Term Paper / Review work – 30 marks, Seminar on

the topic – 10 marks & Viva-voce – 10 marks.

MBIOC0402

BIOINFORMATICS, BIOSTATISTICS & RESEARCH METHODOLOGY


Theory:

1. Descriptive statistics: Population, sample, variables, attributes. Data, collection and presentation of

data, Primary data and secondary data. Diagrammatic representation of data. Tabulation and

graphical representation of data. Mean, median, mode, mean deviation, standard deviation and

standard error; coefficient of variance, quartile deviation. Skewness and measures of skewness.

2. Bivariate frequency distributions: Bivariate data, scatter diagram, concept of correlation and

regression; methods of least squares.

3. Theoretical distributions: Binomial, Poisson and Gaussian.

4. Sampling theory and test of significance: Systematic, random and stratified sampling. Null and

alternative hypothesis, type-I and type-II error, critical region. Chi-square and Student t-test.

5. Analysis of variance: One way and two way classified data with one observation per cell.

6. Programming Language: Basic concepts of programming languages like C and R.

7. Database: Genome databases, protein databases, other secondary databases.

8. Sequence alignment: Idea about the algorithms used in BLAST and FASTA; concept of multiple

sequence alignment.

9. Phylogenetic analysis: Different Methods and its importance.

Practical:

1. Using MS Excel sheet, STATISTICA, Sigma Plot and R

2. Chi square test, Z test and ANOVA using MS Excel and R

3. Database similarity searching for nucleotide and protein sequence using known sequences using

BLAST N and BLAST P program.

4. Phylogenetic analysis using multiple sequence alignment

5. Protein secondary and tertiary structure prediction

6. Retrieval of 3D structure or protein and their respective co-ordinate files from PDB and visualization

using RasMol.

Suggested reading: Statistical Methods- N.G.Das, Tata McGraw Hill pub

Biostatistics- How it works- S.Selvin, Pearson pub

Basic Statistics- A.M.Gun, M.K.Gupta, B.Dasgupta, World Press pub

Probability and Statistics- K.K.Mukherjee, New Central Book Agency pub

Biostatistical Analysis- Jerrold H. Zar, Pearson pub

Introduction to Biostatistics- P.K.Banerjee

Bioinformatics- S.C.Rastogi, N.Mendiratta and P.Rastogi, Prentice Hall

Bioinformatics- T.K.Attwood and D.J.Parissmith, Pearson Education

Bioinformatics Computing- B.Bergeron, Pearson pub

Bioinformatics- David W Mount, CBS pub

Note:


th and 16









MBIOC0403

GENETIC ENGINEERING


Theory:

1. Fundamentals of the Molecular manipulation of DNA: Various tools and techniques.

2. Molecular cloning of DNA: Enzymes involved – DNA ligases, topoisomerases, gyrases,

methylases, nucleases; restriction endonuclease and restriction modification system; general

overview of host and vectors; cloning based on plasmids, bacteriophages, cosmids, M-13,

phagemid, BAC, YAC, shuttle vectors, expression vector.

3. Transgenesis: Different methods of transformation -chemical method, eletroporation,

microinjection and gene gun; Agrobacterium mediated transformation; transgenic plants (disease

resistance, protein production, herbicide resistance); animals as bioreactors.

4. Techniques involved in Recombinant DNA technology : Protein and nucleic acid blotting -

southern, northern and western; construction of libraries - genomic and cDNA, screening; DNA

sequencing; genetic fingerprinting; gel retardation and DNA foot printing; PCR, RT-PCR and Q-

PCR; site directed mutagenesis and protein engineering; FISH; gene knockout technique; DNA

microarray.

5. Gene therapy: General principles; methods for gene targeting; gene therapy in cystic fibrosis,

DMD and SCID; RNAi as a tool of gene therapy.

6. Stem cell therapy: Basic concept of stem cell therapy.

7. Process technology: Production of recombinant therapeutic proteins e.g. insulin, erythropoietin,

factor VIII and IX; Hepatitis and surface antigen; production of amino acids, alcohol and vinegar

by fermentation.

Suggested reading:

Principles of Gene Manipulation and Genomics- S.Pimerose and R.Twyman, BlackWell pub

Recombinant DNA- J.D.Watson, M.Gilman, J.Witkeski and M.Zoller, Scientific American

From Genes to Clones- E.L.Winnacker, Panima pub

Molecular Biotech. and Principles and Appl. of Recomb. DNA- Glick and Pasternik, ASM Press

Gene Cloning, T.A.Brown- Stanley Thrones pub


Recombinant DNA and Biotechnology- H.Kreuzer and A.Massey, ASM Press

Essential Genetics-A Genomics Perspective- L.Hartland W.Jones, Jones and Bartlet pub

Genome Mapping and Sequencing-I.Dunham, Harizon Scientific press

Note:

Mid semester tests shall be conducted during the 8th and 16th weeks of the semester period on the





MBOIC0404

MICROBIOLOGY


Theory:

1.Historical Perspective: Landmark discoveries relevant to the field of microbiology- contributions of

Louis Pasture, Robert Koch, Edward Jenner, Alexander Flemming, Iwanowsky.

2.Methods in Microbiology: Pure culture techniques; methods of sterilization (physical and chemical);

enrichment culture technique; Lyophilization technique, basic principles of Light microscopy, Phase

contrast microscopy & Electron microscopy.

3.Microbial Taxonomy and Diversity : Bacteria, Archaea and their broad classification; Bergey’s

Manual; Characteristics of eukaryotic microbes- fungi, algae and protozoa; Viruses and their

classification(as per ICTV); molecular approaches to microbial taxonomy; brief ideas on prions, viriods

and virusoids.

4.Prokaryotic Cell structure and functions: Brief idea about the structures and functions of bacterial

cell wall, cell membrane, ribosome, flagella, pili, capsule, nucleoid, plasmids, endospore, cell

inclusions.

5.Microbial Nutrition and Growth : Major types of micro-organisms based on modes of nutrition;

macro-and micro-nutrients, Definition of growth, Bacterial growth phases and growth curve,

mathematical expression of exponential growth phase, batch culture, continuous culture, effect of

environmental factors(Temperature, pH, oxygen, osmotic pressure) on bacterial growth.

6.Microbial Metabolism: Outlines of Glycolysis, TCA Cycle, Entner-Duodoroff pathway; Glyoxylate

pathway; fermentation (only homo & heterolactic); nitrate respiration; chemolithotrophy-definition and

mechanism of sulfur metabolism.

7.Medical Microbiology: Normal microbial flora of human body(skin, respiratory tract, GI tract and

UG tract); mechanism of microbial pathogenicity; Nosocomial infection; emerging infectious diseases;

Examples of human diseases caused by viruses, bacteria, fungi and protozoa.

8.Antibiotics: General characteristics and classification of antibiotics; modes of action of anti-bacterial

(Penicillin and Streptomycin), anti-fungal (Amphotericin B and Azoles), anti-protozoal

(Metronidazole) and Anti-viral (AZT) drugs.

9.Microbial Genetics :; Brief idea on Bacterial genetic recombinations - transformation, conjugation

and transduction; mechanisms of replication of retro-virus and polio virus, lytic and lysogenic cycle of

phages; Effects of mutations on micro-organisms(concepts of auxotrophs, prototrophs, conditional

lethal mutants, regulatory mutants); Ames test for mutagenesis; Replica Plating method.

10. Microbial Ecology: Microbial interactions; role of micro-organisms in carbon, sulphur and

nitrogen cycles; VAM and its importance, MPN method for E.coli detection, membrane filter method,

IMViC reactions.

11. Fermenter: Basic design and working principles.

Practical:

1. Pure culture techniques: Spread plate, Pour plate and Streak plate methods.

2. Preparation of agar slant and stab.

3. Isolation and enumeration of bacteria from soil & water (on Nutrient Agar); study of colony

features.

4. Isolation of fungi from soil & water (on PDA).

5. Staining methods: Simple staining (bacteria/fungus), Gram staining (bacteria) and Endospore

staining (bacterial spore).

6. Physiological and biochemical tests – IMViC tests, amylolytic test, proteolytic test, and catalase test.

7. Microbial assay of antibiotics: tube dilution assay (min - inhibiting conc.), disk assay & agar cup

assay.

8. Microbiological examination of water: MPN (presumptive test, confirmation test & completed test)

9. Isolation of bacteriophage from sewage.

Suggested reading:

Brock Biology of Microorganism- M.Madigan, J.M.Martinko and J.Parker, Prentice Hall

Alkamo’s Fundamental of Microbiology- J.C.Ponnerville, Johnes and Barlet pub

Microbiology- L.M.Prescott, J.P.Harley and D.A.Klein, McGraw Hill

Microbiology- N.J.Pelczar, S.Chand, R.Krieg, Tata McGraw Hill

General Microbiology- R.Y.Stanier, L.Ingraham, L.Wheelis and R.Penter, McMillan Press

General Microbiology- H.G.Shclegel, Cambridge

Fundamental Principles of Bacteriology- A.J.Salle, Tata Mcgraw Hill

Microbiology- G.Black, John Willey and Sons

Microbiology-A Introduction- G.J.Tortora and B.R.Funke, C.L.Case, Pearson

An Introduction to Microbiology- P.Tauro, K.K.Kapoor, K.S.Yadav, New Age International

Introduction to Microbiology- J.L.Ingraham and C.A.Ingraham, Thomsone pub

Fermentation Microbiology and Biotechnology- E.Elmanisi and C.Bryce, Trailor and Francis Industrial

Microbiology- Waits

Principles of Fermentation- A.Whitaker, P.F.Standbury, S.J.Hall, Aditya Books Pub

Industrial Microbiology- L.E.Casida, JR., New Age International

Introduction to Modern Hydrology- J.Dimmock, A.J.Easton and K.N.Leppord, BlackWell pub Medical

Microbiology- G.F.Brooks, J.S.Butel and S.A.Morse, McGraw Hill

Note:


th and 16









MBIOC0405

GENERAL BIOCHEMISTRY


Theory:

1. Bioenergetics: Cell as an energetic system - application of first and second laws of Thermodynamics, cell as

an open system, dynamic equilibrium, chemical potential, water potential in water relations, chemical

potential of ions; free energy in biochemical reactions, phosphate transfer and phosphorylation, redox systems

and redox potential; ATP synthesis and hydrolysis, high energy bonds in biomolecules. Glycolysis, TCA

cycle, Electron transport chain, oxidative phosphorylation.

2. Carbohydrates: Definition; structure of carbohydrates- monosaccharide, aldohexoses and Ketohexoses;

Haworth Structure Anomeric structures of D-Glucose, mutarotation, pyranose and Furanose rings; examples

of different types of monosaccharide. Oligo- and polysaccharides- reducing (maltose) and non-reducing

(sucrose), disaccharides; Glycoproteins, proteoglycans.

3. Lipids: Definition, distinction between fats and oils, structure of lipids (fatty acids, glycerolipids,

sphingolipids).

4. Amino acids, peptides and proteins: Structures and important properties, Classification of amino acids,

important physical and chemical properties of amino acids (optical isomerism, UV-absorption region,

ionization, reactions due to amino group and carboxyl group). Primary structure of peptides and proteins-

peptides bond formation, hydrolysis of peptides and proteins. Primary, secondary, tertiary and quaternary

structures. Classification of proteins (based on solubility and composition). C and N terminal amino acid

determination.

5. Nucleic acids: Structure of nucleic acids; nucleosides, nucleotides, primary structure, A, B and Z form of

DNA. A preliminary idea of secondary structures of RNA and DNA. Melting point and Denaturation of DNA.

6. Enzymes: Definition of enzymes, important terms (enzyme unit, specific activity). Classification of enzymes;

physico-chemical properties, factors affecting activity; mechanism of enzyme action, coenzymes, cofactors.

Suggested reading:




Principles of Biochemistry- H.R.Horton, L.A.Moran and G.Scrimgeour, D.Perry, D.Rawn, Prent Hall

Out lines of Biochemistry- E.E.Conn, P.K.Stumph, G.Bruening and R.H.Doi, John Willey and Sons

Instant notes of Biochemistry- B.D.Hames and N.M.Hooper, Viva Books

Thermodynamics for Chemists- S.Glasstone, Affiliated East-West Press

An Introduction to Thermodyanamics- Y.V.Rao, Universities Press


Principle of Biochemistry – J. L. Jain, V. K. Jain

Textbook of Biochemistry – U. Satyanarayana

Biochemistry – D. Das

Textbook of Biophysics and biophysical chemistry – D. Das

Note:


th and 16

th weeks of the semester period on the topics of this

paper. The marks obtained will be considered for the final awarding.

Continuous Assessment: [C1= 2.5 marks, C2= 2.5 marks & C3= 20 marks (Theory: 20 marks)].

Marks distribution (Theory): 01 question of 08 marks (out of 02), 02 questions of 04 marks (out of 04) & 04

questions of 01 mark (out of 06).

BU M.Sc. Biochemistry Syllabus

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