Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research
Dec 17, 2015
Breast Cancer ECM Visualization using Multiphoton Imaging
Jeremy BredfeldtLOCI, Morgridge Institute for
Research
H&E and SHG
• Protocol– Thaw & fix sample– Section sample to 200 microns on a vibratome– Image with SHG– Paraffin embed tissue wafer– Section and stain for H&E– Slide scan
• Green = SHG signal (Collagen Type I)
Normal Associated Tissue
11.6 mm
H&E
SHG
Invasive Ductal Carcinoma
H&E
SHG
Fibroadenoma
H&E SHG
7.5 mm
Mucinous Carcinoma
H&ESHG
Intrinsic Fluorescence
• Green = SHG• Red = Autofluorescence from FAD or Collagen
type III
FibroAdenoma
Extrinsic Fluorescence
• Green = SHG (Collagen type I)• Red = E-Cadherin stained cells &
Autofluorescence from Collagen type III
Terminal Ductal Lobular Unit (Red) Surrounded by Collagen (Green)
Duct in Cross Section, Surrounded by IDC cells
IDC
Duct
IDC Cells Near a Vessel with ECM
IDC Cells
Vessel