Blood Collection & Processing

8/12/2019 Blood Collection & Processing

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BLOOD

COLLECTION AND

PROCESSING

M KASTHURI BAI


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OBJECTIVE

After following this lecture, students must

be able to understand:

Processes involved in blood donation

center.

Important criteria in donor selections. The donor unit processing.


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LECTURE CONTENT

Donation process

Registration

Donor selection

Medical history Physical examination

Phlebotomy

Adverse donor reactionDonor unit processing

Storage of donated blood


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Donation process

Donor area - attractive

- open at convenient hours

- comfortably ventilated

- understanding

- professional

- well trained personnel


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Registration

Information fully identify the donor

Details

Date & time donation Name and address

Contact no.

Gender

Age/ date of birth

Previous transfusion/ TTD


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Donor selection

Important in protecting safety of blood

supply

To obtain truthful & accurate medicalhistory to ensure safe transfusion

Protect donor confidentiality

Uniform donor history questionaire


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Physical examination

Donor should be in good health

Donor deferred if under influence of

alcohol or drugs or with obvious signs of

cold or other illness


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Determination of Hb and hct levels

Hb : 12.516.0 g/dl (female)

: 13.518.0 g/dl (male) Blood pressure

Systolic : < 180 mmHg

: < 100 mmHg

Pulse

Pulse rate should be between 50100

beats per minute


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Temperature

Orally should not exceed 37.5C (99.5 F)

Weight 50 kg (110lb ) or more450 mL of blood

< 50 kgnot more than 12% of blood volume

Formula : Body wt(lbs) x 450 = allowable amt

110 (lbs)

Skin lesionfree at venipuncture


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Phlebotomy

Bag selection

Identification

Selection of vein Phlebotomy & collection of samples

Care of donor


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Bag selection

FDA approved

Pyrogenfree, sterile, sufficientanticoagulant

According to need and amount ofcollection

Single / triple


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Identification

Assigning identification number to each

individual donation

Allows donation process to be tracedand tracked

Numeric or alphanumeric no should be

placed on donation record, primary bag,

all satelite bags and sample tubes


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Selection of vein

Both arm inspected for lesions that may

indicate IV drug use or signs of infection

Blood drawn from large, firm vein in theantecubital area of the arm

Tourniquet or BP set inflated to 40

mmHg

Aseptic technique must be used


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Phlebotomy and collection of

samples Final check to verify donors

identification

During collection blood mixed withanticoagulant

Amount collected monitored carefully so

total does not exceed 525 mL

PPE


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Care of donor

After removing needle from vein

Apply firm pressure with sterile gauze

Donor recline on bed of donor chair for few

minutes Allow to sit. Follow donor to refreshment

area

Post phlebotomy care

Eat and drink before leaving

Drink more fluid in next 4 hours

Avoid alcohol until you have eaten


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Care of donor

Do not smoke for 30 minutes

If bleeding persists, raise hand and apply pressure

Fainting/ dizziness occurlie down with head btwknees

If symptoms persists contact BDC

Resume all normal activity if asymptomatic

Remove bandage within few hours Maintain high fluid intake for approx 3 days

THANK DONOR & ENCOURAGE REPEAT DONATION


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Adverse donor reaction

Personnel should be trained in CPR

Syncope (fainting)

Caused by sight of blood

Symptom: weakness, sweating, dizziness,pallor. Unconcious

Deep breathing and hyperventilation

Fainting

Place donor on back with feet raised above head Loose tight clothing

Adequate airways

Aromatic spirits


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Donor unit processing

ABO & Rh grouping

Antibody screening

Screening of transfusion transmitted

diseases Syphilis

Hbs Ag

Anti HCV

Anti HIV

Anti HTLV I / II

CMV


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LECTURE CONTENT

Donation process

Registration

Donor selection


Phlebotomy




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Blood unit Storage condition Storage duration

Red blood cell 14 C Max 42 days

Platelets RT 5 days on agitator

Fresh frozen plasma frozen 1 year

Cryoprecipitate frozen 1 year

Granulocyte RT Transfused within 24

hours


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Thank you

Blood Collection & Processing

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