BIOTECHNOLOGY - DOST PCIEERDpcieerd.dost.gov.ph/.../pdf/2017/roadmaps/Biotechnology.pdfCompetitive and Sustainable Agriculture and Fisheries Sectors for Food Security and Increased

BIOTECHNOLOGYR&D ROADMAP

(2013-2019)

DEPARTMENT OF SCIENCE AND TECHNOLOGY

AGRICULTURE

Competitive and Sustainable Agriculture

and Fisheries Sectors for Food Security and Increased Productivity

AGRICULTURE : CROPSABACA and COCONUT

COCONUT: M.M. for dwarf variety, developmental processes, oil and lipid production, and metabolic

processes (JAN 14, 2017)[2]Transcriptome sequence of 8 coconut varieties & putative gene markers for early

flowering, fast-growing, high nut yield and water content & quality (JAN 14, 2017)[4]

ABACA: M.M. for fiber quality & virus resistance (APRIL 30, 2014)[1]

COCONUT: M.M. for early flowering, fast growth, oil and nut yield and water content and quality (JAN 14, 2019)[3]Varieties from M.A. breeding of Tall x Tall and Tall x Dwarf (JAN 14, 2019)[7]Genetic linkage map derived from validated QTLs for routine M.A. breeding (JAN 14, 2019)[8]Markers and NGS-Eco TILLING platform for glandular trichome genes; SNP markers for coconut scale

insect resistance gene (JAN 14, 2019)[9]COCONUT: Estabm’t of breeding population [3]

ABACA: Identificat’n of genes for fiber quality & ABTV resistance and construction of genomic DNA library/ database[1]

ABACA: Development of SSR based primers from the NGS Abuab and gDNA library [1]

Selection & nationwide replanting of genuine PCA hybrid seed nuts & 'San Ramon' composite variety [7]Dev’t of genetic linkage map of coconut for High Yield and Outstanding Quality of Copra Oil [8]Charact’n of coconut glandular trichome loci/genes tagged with sequence-specific DNA markers[9]

COCONUT: Generating contig assemblies of the transcriptomes of normal,

makapuno and lono phentoypes [5]Gene discovery of at least 5 coconut genes involved in several

metabolic pathways [6]Dissemination of Outstanding Tall coconut selections in target

growing regions based on genetic relationships [7]Dev’t of polymorphic DNA markers bet. parental populat’ns [8]Construct’n of web-based genome database of Cocos nucifera [9]

COCONUT: Establishment of biomarkers for early flowering, fast growth,

oild & nut yield and water content & quality. [3]Genomic sequencing of Laguna Tall (LAGT) and Catigan

Green Dwarf (CATD) of Cocos nucifera [2]Purification of total RNA from 8 rep coconut varieties [4]

Dev’t of markers for dwarf variety, developmental processes oil & lipid prod’n & metabolism[2]Dev’t of primers for t M.A. breeding, hybridity testing, forensics, fingerprinting, genotyping [3]Dev’t of draft transcriptome sequences of the 8 coconut varieties & putative gene markers [4]Comparison of listings and Identificat’n of candidate genes and relevant gene networks [5]Dev’t of at least 5 constructs with one or two genes for oil biosynthesis [6]

Regeneration of transformed tissues into whole plantlets in bottles [6] Dev’t of composite/synthetic varieties from M.A. breeding [7] Mapping of QTLs of productivity & yield/quality of copra oil & by-products [8] NGS-Eco TILLING platform for glandular trichomes genes [9]

COCONUT: Dev’t of linkage map early flowering, oil, nut yield, H2O content [3]Ident’n of early flowering, high nut yield, H2O content & quality[4]Dev’t of M.M. for use in M.A. selection and breeding [5]M.M. for oil biosynthesis of high oil-yielding, makapuno

& lono coconuts (JAN 14, 2017) [5]Genes involved in metabolic pathways & oil

biosynthesis (JAN 14, 2017) [6]

COCONUT:

Dev’t of robust markers for routine M.A. breeding [8] Eval’n of plant/s that exhibit differential reaction

against scale insect infestation [9]

Competitive and Sustainable Agriculture and Fisheries Sectors for Food Security

and Increased Productivity

Extract’n of total RNA & generation of transcriptomics sequence data using a appropriate NGS Platform [11]DNA Fingerprinting of entries using the established markers for sucrose content, smut

and downy mildew) to produce a molecular database of the varieties [12]Evaluation of the clones through Brix and morpho-agronomic characteristics [13]

AGRICULTURE : CROPSCOFFEE, SUGARCANE, RICE, RUBBER and CACAO

RUBBER: Validated 185 rubber nurseries in North

Cotabato, Zamboanga Peninsula, Caraga, Sultan Kudarat, Palawan, Quezon,Kalinga Apayao, & Nueva Viscaya (MAR 2015)[16]gDNA libraries (APRIL 2015)[19]

RUBBER & CACAO : Designed primers (DEC 2017)[19]

SUGARCANE: M.M. for High Sucrose Content,

Resistance to Smut & Resistance to Downy-Mildew (AUG 16, 2016)[11]Protocol of M.A. nobilization for

improvement (AUG 16, 2016)[12]5 New Improved Varieties with high

sucrose content & resistance to fungal diseases through MAS (AUG 16, 2016)[13]

COFFEE: Identf’n of M.M. for resistance to pests (berry borer & scale insect), diseases (leaf blight & rust), yield, and aroma of NSIC varieties & strains [10]

RICE: Testing the induction of resistance in 3 susceptible hybrid rice varieties & Field experiments and advance evaluation in farmers field [15]

RUBBER & CACAO: Prep’n for 2 gene-enriched gDNA libs & Isolation of 100 transformed bacterial colonies [19]

RUBBER & CACAO : Validated PCR primers for possible two (2) trait association (JAN 2018)[19]

RUBBER & CACAO : Selection of SSR markers from exome databases for primer design (gene mining) [19]

RUBBER & CACAO: Sequencing of 200 colonies, identified SSRs from sequenced colonies and designed primers; test designed primers to the 10 NSIC varieties [19]

RUBBER: Baselined rubber plantations/nurseries with high yielding rubber clones from the target nurseries, Identification of clones and SSR analysis [16]

SUGARCANE: Const’n of RNA sequence data for mined

information and database [11]Identf’n of accessions with markers for most

traits & interspecific for parental purposes [12]Identf’n of promising sugarcane clones with high

sucrose content, and resistant smut and downy mildew through the use of MAS [13]

Determt’n of the effect of chitosan and carrageenan application on rice growth, nutrient uptake, and physiological process [14]Testing Induction of resistance, dosage refinement, and

frequency of application [15]

SUGARCANE: Testing and validation of some of these markers in sugarcane breeding populations/ lines[11] Evaluation of the effectivity of markers in identifying the true hybrids [12] Field testing of promising varieties [13]

CACAO: Gene-enriched gDNA libraries for cacao (APRIL 2015)[19]

COFFEE: M.M. for pest/disease resistance,yield & aroma (OCT 2016)[10]RICE: Technology on Radiation-

Modified Carrageenan & Chitosan Rice management (MAY 16, 2016)[14]Varieties resistant to tungro

disease & best pest treatment (MAY 16, 2016)[15]

AGRICULTURE : CROPSMANGO, SQUASH and TOMATO High Yielding and Pest & Diseases

Resistant crops

MANGO: Re-evaluation of mango collections with peel color, thickness, and resistance to anthracnose, stem-end rot, fruit fly, cecid fly, leaf hopper [18*]

MANGO: DNA markers for ‘Carabao-Mango’(FEB 28 2014)[17]

MANGO: Database of morphological and molecular characteristics (2017)[18*]

MANGO: Diagnostic kit (dipstick kit) for Ident’n of ‘Carabao-Mango’ (FEB 28 2015)[17]

MANGO: Testing of the diagnostic kit (dipstick kit) in mango nurseries [17] SQUASH: Dev’t of populations resistant to SLCV, ZYMV & PRSV-W [17] TOMATO: Dev’t of DNA markers tagging the viral resistance gene sequences [21]

MANGO: Development and identification of DNA molecular markers, database of molecular characteristics and population structure of Philippine mango cultivars [17]

SQUASH: Determination of predominant strains of squash-infecting Begomovirus and development of infectious DNA clones of local strains of the major squash virus, the Squash leaf curl virus (SLCV) [20]

TOMATO: Screening the loss of susceptibility mutants of tomato against TYLCV/ToLCV, CMV [21]

MANGO: Ident’n of ‘Carabao’ mango specific proteins for dev’t of diagnostic kit (dipstick kit [17] SQUASH: Identify sources of genetic resistance to SLCV, the Zucchini yellow mosaic virus (ZYMV),

and the Papaya ringspot virus type W (PRSV-W) in collaboration with AVRDC [20] TOMATO: Conduct molecular characterization of induced resistance gene sequences [21]

MANGO: M.M for red blush, thick peel, resistance to insect pests (fruit fly, cecid fly, leaf hopper) and major diseases (anthracnose and stem-end rot) (2016)[18*]

MANGO: Ident’n of M.M. in varieties associated with key traits [18*]

* Proposed Project

TOMATO: Allelic variant resistant to TYLCV/ToLCV & CMV (MAR 2013)[20]

SQUASH: Six (6) germplasm resistant to SLCV, ZYMV & PRSV-W (MAR 2014)[20]TOMATO: Characterized virus resistance

(MAR 2014)[21]

SQUASH: One (1) improved population and 1 open-pollinated variety moderate resistance to SLCV, ZYMV, and PRSV-W (MAR 2015)[20]TOMATO: DNA markers tagging the virus resistance (MAR 2015)[21]

TOMATO: Multi-location field trial and Plant Variety Protection (PVP) filing towards commercial varietal release and Genomics & variability of Tomato leaf curl Philippine [22*]

TOMATO: M.A. breeding for leaf curl virus resistant (2019)[22*]

TOMATO: Commercial release of virus resistant tomato(2018)[22*] Spin-off negotiations with local & intern’l breeding institutions/seed companies (2018)[22*] Genome charact’n & extent of genetic diversity among ToLCPHV species in the Philippines (2018)[22*]

TOMATO:Open-pollinated varieties and hybrids of fresh-market and processing resistant to ToLCPHV (2019)[22*]Genetic population/s of a fresh-market & processing for the target disease resistance and abiotic stress tolerance traits (2019)[22*]

SQUASH: One (1) DNA clone for predominant strain of SLCV (MAR 2013)[20]

Genomics-assisted mapping & pyramiding of disease resistance & abiotic stress tolerance genes (2019)[22*]

AGRICULTURE : LIVESTOCK & POULTRYGOAT, WATER BUFFALO, SWINE, POULTRY and MEAT PRODUCTS

Competitive and Sustainable Agriculture and Fisheries Sectors for Food Security and Increased

Productivity

MEAT PRODUCTS: Testing & Valid’n of PCR and LAMP based methods [4]

SWINE: Pilot scale at capacity of 300 units/yr & prod’n of PED LAMP test kits [6]

WATER BUFFALOES: Equation for predicting genetic merit of animals based on BLUP EBVs and GEBVs and Selection index for bulls and heifers incorporating BLUP EBVs and GEBVs. (NOV 30, 2017)[7]

MEAT PRODUCTS: Dev’t of LAMP-based species ident’n protocol (4 species)[4]

SWINE & POULTRY: Eval’n of PECM in commercial swine and poultry farms [5]

WATER BUFFALOES: DNA sampling, extraction, genotyping & data eval’n [7]

GOAT: Primer design of the CAEV strains and field validation. [1]

SWINE & POULTRY: Estab’t of pilot scale prod’n system for protein enrichment of copra meal [5]

GOAT: Measurement of efficiency, reliability & validation of the designed LAMP assay for CAEV followed by the Charact’n/testing of CAEV [1]

MEAT PRODUCTS: DNA barcode sequence of 12 animal species) (MAR 31 2016)[4]SWINE: Policy brief to institutionalize the adoption of LAMP assays by state-based animal disease diagnostic

laboratories (JUN 30 2016)[6]WATER BUFFALOES: genetic architecture of the Phil. dairy population (JUN 30 2016)[6]

WATER BUFFALOES: Dev’t of model for predicting genomic breeding values for marker genotypes using Valid’n of prediction eq’n [7]

GOAT: LAMP Protocol for the detection & screening of CAEV (JUL 16 2013)[1]

SWINE: LAMP Protocols for detection of PED virus, Salmonellosis & Cryptosporidiosis & LAMP test kit for PED virus (SEPT 30 2014)[2]Gene markers for ident’n of positive & disease resistance/ screening of genetic defects(DEC 31 2014)[3]LAMP Protocols respiratory & gastrointestinal diseases via “lab in a mug” heat blocks (JUN 30 2014)[6]

SWINE: Genetic data on the presence or absence of positive/negative genes (DEC 31, 2015)[3]Packaged LAMP test kit for PED virus (for pilot scale production) (JUN 30 2014)[6]

MEAT PRODUCTS: 4 sets of species-specific LAMP primers & 12 pairs of species-specific PCR primers (MAR 31 2015)[4]SWINE & POULTRY: PECM as a new feed product (DEC 31, 2015)[5]WATER BUFFALOES: SNP markers for milk production traits (DEC 15, 2015)[7]

SWINE: LAMP primers for PED virus, Salmonellosis & Cryptosporidiosis (SEPT 30 2013)[2]

Design of LAMP primers & for PEDV, Salmonellosis, Cryptosporidiosis [2]Ident’n & optim’n of gene primers of positive traits (litter size, growth rate, meat quality, muscle mass), disease resistance (salmonellosis, PRRSV, intracellular pathogen, E. coli and influenza) & screening of genetic defects (acid meat, stress syndrome and scrotal hernia) [3]

SWINE & POULTRY: Bioprocessing technology for protein enrichment of copra meal (DEC 31 2014)[3]MEAT PRODUCTS: LAMP ident’n protocols & test

kits (MAR 31 2014) [4]

SWINE:Opt’n of LAMP protocols, Dev’t & valid’n

of LAMP-based quick test kit [2]Blood sample coll’n, DNA extraction and

Genotyping of DNA samples to identify positive, disease resistance genes and screen genetic defects [3]Re-optimization of PED LAMP protocols

using fabricated “Lab in a Mug” heat block [6]

GOAT: LAMP primers and Phylogenetic Analyses for CAEV, (JUL 16 2014)[1]

SWINE & POULTRY: Testing & optimization of operating condition in each of the steps in the prod’n system and Establ’t of product (PECM) quality data & analysis [5]

MEAT PRODUCTS: Optim’n of PCR-based species ident’n protocol (for 12 animal species) for fresh meat & meat products [4]

Ass’n analysis of genotype w/ phenotype & Establ’t of genetic service laboratory [3]

SWINE: Field validt’n of PED in Reg. 3 & 4 [6]

WATER BUFFALOES: Genome ass’n studies, comparison of models & Principal component analysis [7]

AGRICULTURE : INLAND AQUATICMILKFISH, MUSSEL SHRIMP and TILAPIA

Competitive and Sustainable Agriculture and Fisheries Sectors for Food Security and Increased

Productivity

MILKFISH: M.M. of best stock for breeding & distinction of wild fry vs. hatchery-bred fry (MAY 2015)[1]Procedure using fish scales to measure/detect early growth rates (MAY 2015)[2]M.M. for lectin to assess innate immunity (MAY 2015)[3]Diagnostic kit for Microbial Infection (MAY 2015)[4]

Utilization and innovation of databases/methodologies and dev’t of local applications to control and/or neutralize WSSV [9]

MILKFISH: Dev’t of microsatellite and RAPD markers for evaluating the impact of domestication on local milkfish hatchery stocks [1]Sequencing of mitochondrial cytochrome b gene and control region [2]Cloning & sequencing of lectin genes from a pooled cDNA library from the liver, kidney, and spleen [3]Utilization of advanced molecular technologies to identify & detect pathogenic bacterial species from diseased fish samples [4]

MILKFISH: Ident’n of highly genetically variable stocks for use in stock replenishment [1]Design gene specific primers based on teleost lectin sequence databases & peptide sequences [3]Dev’t of a molecular diagnostic technology for a rapid and reliable detection of pathogenic species [4]

SHRIMP: Standard protocol for dsRNA mass production (JUN 2016)[9]Protocol on selection/sourcing of P. monodon seedstock (JUN 2016)[10]

MILKFISH: Populations with high genetic variability (MAY 2014)[2]

SHRIMP: Sequencing of 300 pathogens from outbreaks & the field [6] Establ’t of an online Philippine shrimp pathogen database [7] Synthesis of gold metal nanoparticles-DNA conjugates &

incorporation into a lateral flow strip biosensor (LFSB) [8]

MUSSELS: Determt’n of genetic variability from different sites using RAPDs, RFLPs and unique regions of specific genes [5] TILAPIA:

Examination of levels of genetic diversity in improved strains of Nile and red tilapia [11]

Generate scientific data of varieties (GIFT, FAST, GET-EXCEL, SEAFDEC, GST (GenoMar Supreme Tilapia). [12]

SHRIMP: Genome sequence of 300 shrimp pathogens (DEC 2015)[6]Online Philippine Shrimp Pathogen Biobank (DEC 2015)[7]Diagnostic kit - Lateral Flow Strip Biosensor (LFSB) (DEC 2015)[8]

MUSSEL: M.M. for higher survival, faster growth & disease resistance (2015) [5]

TILAPIA: Comparison of Genetic Variation of Philippine Tilapia & Other

countries (i.e. China, Thailand & Malaysia) (SEP 2015)[11]Profile of hatcheries: strain, management applied, maturity

status and production (SEP 2015)[12]

MUSSELS: Identif’n of stains/varieties/ populations for selective breeding and generate a genetic profile for the natural populations of this species. [5]

Utilization of NGS & bioinformatics to conduct “association studies” to identify a gene or a group of genes in tiger shrimp related to high growth, high health, disease tolerance etc [10]

SHRIMP: Dev’t & eval’n LFSB prototype performance for WSSV

screening & field test trials and Dev’t of Nested PCR protocols for the detection of WSSV, IHHNV, MBV and Vibrio spp; [8]

AGRICULTURE : MICROBIALS for crops Competitive and Sustainable Agriculture and Fisheries Sectors for Food Security and Increased

Productivity

STIMULANTS: Microbial phytohormone-based biostimulant (MAY 31 2013)[4]BIOCONTROL: Yeast for postharvest pathogens

of fruits & vegetables (MAY 31 2013)[6]BIOBANK: Characterized Strains & re-identified

taxonomic methods (MAY 31 2013)[8]

BIOCONTROL: Application mode of Biocontrol agent (MAY 31 2014)[6]BIOBANK: New strains isolated with BFSP properties (MAY 31 2014)[8]MYKOPLUS: Increased awareness & adoption and Business plan for

MykoPlus technology (MAY 31 2014)[1]

MYKOPLUS:Quantification of the effect and soil amendments/properties on growth and yield [1] BACTERIAL INOCULANT Formul’n & shelf life assessment of EB inoculant as new biofertilizer [2] BIOFERTILIZER: Formul’n of a consortium of microorganisms & carrier materials & Field testing [3] STIMULANTS: Screening of locally available materials as substrate for phytohormone production [4] BIOBANK: Charac’n & Polyphasic identif’n of strains, biofertilizer, biostimulant & biopesticide [6]

MYKOPLUS (for Yellow Corn & Eggplant): Establishment of demonstration trials and trainings on application [1] BACTERIAL INOCULANT (for Eggplant & Sugarcane): Morphological, biochemical & molecular characterization of EB isolates [2] BIOFERTILIZER: Assessment of the rhizobacterial dynamics in acid soils & Testing of isolates for plant growth promotion [3] STIMULANTS: Construction of phytohormone-hyper-producing consortia for potential phytohormone prod’n [4] BIOCONTROL: Isolation and identification of yeasts with potential antagonistic activity [6] BIOBANK: Preservation of cultures [8]

MYKOPLUS: eterm’n of the economics of using MykoPlus [1] BACTERIAL INOCULANT: Bio-efficacy & Field Testing the

effectiveness of formulated EB in the field; Cost- benefit analysis for using the developed product(s) [2]

MYKOPLUS: Established profitability of MykoPlus (MAY 31 2015)[1]BACTERIAL INOCULANT: Established profitability of Inoculant (MAY 31 2015)[2]BIOFERTILIZER: Established profitability of Biofertilizer(MAY 31 2015)[3]STIMULANTS: Established profitability of stimulants (MAY 31 2015)[4]BIOCONTROL: Established profitability of Biocontrols (MAY 31 2015)[6]BIOPESTICIDE: Most effective carrier forml’n & Established profitability (MAY 31 2015)[7]BIOBANK: M.M. of the new BFSP strains & preserved strains for long-term use & distribution for

researchers (MAY 31 2015)[8]

BIOPESTICIDES: Screening for pesticidal compounds from callus microbe co-cultures[5]Isolation & screening of microbes with presumptive biocontrol action to causal agent of banana Fusarium wilt & bacterial blight of corn (Rhizoctonia solani) & forml’n of the effective biocontrol agents[7]

BIOPESTICIDES: Isolation, charac’n & forml’nn of pesticidal compounds from callus-microbe co-cultures [5] Toxicological eval’n & microbial community population dynamics study as affected by the

introd’n of the biocontrol agents [7]

BIOPESTICIDES: Eval’n of the formulated biopesticide and Cost & return analysis [ 5] Efficacy trials of biocontrol agents & Economic [ 7]

BIOCONTROL: Biomass recovery, formulation & storage stability tests, Laboratory, greenhouse and field tests; Cost benefit analysis [6]

BIOBANK: Dev’t of M.M. accession of strains in the PNCM culture holdings [8]

BIOFERTILIZER: Cost-benefit analysis on the appl’nn of the new microbial inoculant [3] STIMULANTS: Forml’n of microbial phytohormone-based stimulant, eval’n of product

effects & economic analysis on the use of biostimulant product [4]

BIOPESTICIDE:Prod’n Process of biopesticides by callus &

microbe co-culture (MAY 31 2013)[5]Microbial isolates w/ biological control

action (MAY 31 2013)[7]

MYKOPLUS: Mykoplus Primer (MAY 31 2013)[1]

BACTERIAL INOCULANT: Inoculant in Powder Form (MAY 31 2013)[2]

BIOFERTILIZER: Multi-strain biofertilizer (MAY 31 2013)[3]

BIOPESTICIDE: Formulated biopesticides (MAY 31 2014)[5]Toxicological and environmental impact assessment

with biocontrol agent application (MAY 31 2014)[7]

BACTERIAL INOCULANT: Fertilizer w/ EB inoculant (MAY 31 2014)[2]BIOFERTILIZER: Info on soil microbial diversity of the acid soil envi. (MAY 31 2014)[3]STIMULANTS: Fertilizer using chemical fertilizer & biostimulant (MAY 31 2014)[4]

ENVIRONMENT & NATURAL RESOURCES

Identification of the microorganisms effective in accumulating heavy metals and capable of biofilm formation [1] Screening & Identification of Biofilm Formers as Potential Microbial Remediators for Heavy Metal Contaminated Wastewater [2]

Isolation, identification & functional characterization of compounds, enzymes & best genetic flora & fauna varieties

Field-based correlation of gene-markers and best properties of endangered species/varieties.

Development of policy recommendations on bioprospecting

Screening, Isolation, identification & functional characterization of compounds, enzymes & best genetic varieties

Review of regulations on bioprospecting Field or gene-based conservation programs

for identified aquatic/terrestrial species Biotechnology based ecological restoration

of polluted sites

Pilot scale and field testing of compound and enzyme production for bioremediation technologies and industrial, health and agricultural applications

ENVIRONMENT & NATURAL RESOURCES : Biodiversity Conservation and Bioremediation Biotech

Conservation of Philippine Biodiversity for Bio-remediation,

Industrial, Health and Agricultural Applications

Field-based, gene-based conservation programs for identified aquatic, marine and terrestrial speciesBiotechnology based ecological restoration of polluted sitesReview of regulations on bioprospecting

Bacteria forming Biofilms for heavy metal absorption (Dec 31, 2013)[2]

Microorganisms effective in accumulating heavy metals and capable of biofilm formation (Feb 14, 2014)[1]

Database for conservation, source of high quality seeds, potential industrial uses bioremediation technologies for contaminated wastewater and soilField-based, gene-based conservation programs for identified aquatic, marine and terrestrial speciesBiotechnology based ecological restoration of polluted sitesDevelopment of policy recommendations on bioprospectingGenetic varieties of endemic species, ecotourism flora and fauna

Compound /enzymes for bioremediation technologies,industrial, health & agricultural applications

National Greening Program for reforestation project; database for conservation, source of high quality seeds, potential industrial uses Biosensor & test kits, for marine toxins photo bioreactor, biofertilizers, biofilters,

identified species for bioremediation, marine detrivores & filter feedersGenetic & hydrodynamic connectivity patterns within archipelago and region,Baseline genetic diversity & resilience estimates for selected indicator species

Gene sequencing design, run & data analysis, identification of key genes of plants, animals & microorganisms for conservation, Disaster mitigation and remediation

Key genes of plants, animals & microorganisms for conservation, disaster mitigation & remediationDatabase of identified species and their genome

Compounds & enzymes from best genetic varieties propagation protocol

Field-based correlation of gene-markers & best properties of identified speciesGene products from identified M.M. for Isolates microbial culture collectionCompounds and enzymes from best genetic flora & fauna varieties Harmful algal blooms detection

ENERGY

Efficient and Sustainable Alternative Fuel Source

Gene sequencing design, run & data analysis, & identification of key genes of marine & microbial samples for bioenergy.

Genetic engineering of microorganisms to express enzymes that produce ethanol for biofuels

Pilot-scale genetically engineered microbial production of ethanol & lactic acid for biofuels.

Performance data at bench scale Algal based bioethanol using improved strainsAlgal based biodiesel using improved microbial strains

Isolation, chemical, biochemical & functional characterization of genes, small molecules & enzymes for bioenergy

Energy biomass & substrate optimization for ethanol & lactic acid production for biofuels

Small-scale genetically engineered microbial production of ethanol for biofuels

Optimized Conditions for Pretreatment, Saccharification & Fermentation of Lignocellulosic Feedstocks (Mar 31, 2015)[1,3]

Identified Microbial Strains for bioethanol from a. Lignocellulosic feedstocks (Mar 31, 2015)[2]b. Sugar-based feedstocksc. Macroalgae

Nanostructrure of microbial strains - Microbial strains for biodiesel from microalgae

Development of Microorganisms Capable of Utilizing Lignocellulosic Hydrolysates for Fuel Ethanol Production[2]

Identification and evaluation of appropriate enzymes and the appropriate pretreatments, saccharification and fermentation conditions for specific lignocellulosic feedstocks[1,3]

ENERGY: BIOFUELS

HEALTH

Small scale validation, stability testing and community-based testing of the POC kit [1]

HEALTH Harnessing Biotechnology to

Boost National Competitiveness in the

Development of Health Care Solutions and Services for

Inclusive Growth

Prototype of the Lateral POC Sytem (Dec 31, 2014)[1]Data on the advantage of MODS Assay in

terms of rapid production of test results and major advantage in terms of performance, efficiency, and cost effectiveness (March 2014)[1]

Production, screening and characterization of immuno reagents for the lateral flow test [1] Design and development of a Lateral POC System[1]

Validated and stable Lateral POC system for the detection of dengue (June 30, 2014)[1]Genomic SNP markers for cardiovascular conditions, and responses to treatment for

cardiovascular diseases (June 2016) [3]SNPs Database for cardiovascular disease, hypertension and dyslipidemia (June 2016) [3]Biomarkers in the form of genetic and transcriptomic biomarkers that are predictive of T2DM,

related medical conditions, complications and of clinical responses to various treatment (Mar 2016) [4]

Optimization of Biotek-M dengue-seco kit , Stability testing of Biotek-M dengue-seco kit [2]

Biotek-M Dengue-seco Kit (Lyophilized Format) (March 30, 2015) [2]

Genotyping, SNP Screening, Processing of samples for microarray procedures (Hypertension, Coronary Artery Disease & Dyslipidemia) [3]

Extraction of DNA, processing and documentation of samples for quantity & purity (Hypertension, Coronary Artery Disease and Dyslipidemia) [3]

Extraction of DNA, processing and documentation of samples for quantity & purity (Type 2 Diabetes Mellitus) [4]

Comparative laboratory performance testing between dengue-seco against the liquid format [2]

Criteria for evaluation are specificity and sensitivity of the tests as well as convenience, efficiency, and cost-effectiveness.[6]. Comparative evaluation of TB diagnostic tests such as the gold standard Lowenstein-Jensen Method, the automated MB

Bac/t system, and the MODS Assay for the determination of TB and MDR-TB positive among sputum samples from the PGH and the NTRL Laboratories [6].

Genotyping for Type 2 Diabetes Mellitus, SNP Screening, Processing of samples for microarray procedures [4]

Validation of published mutations in the KRAS, PIK3CA, BRAF, PTEN and AKT1 genes in Filipino colorectal cancer patients through next generation sequencing technology. (Dec 2017) [5]Detection of novel mutations in colorectal cancer tumors that can be further characterized in order to determine their

molecular and biological functions in cancer development. (Dec 2017) [5]

Patient recruitment, sample collection & baseline data collection for 2,850 participants (Hypertension, Coronary Artery Disease, Dyslipidemia) [3] and for 2,062 participants (Type 2 Diabetes Mellitus) [4]

Identification of mutations in the KRAS, PIK3CA, BRAF, PTEN and AKT1 genes will be determined via targeted sequencing (Colorectal Cancer) [5]

Assessment of the mutations in the KRAS, PIK3CA, BRAF, PTEN and AKT1 genes using various mutational assays (Colorectal Cancer )[5]

These markers can be translated to diagnostic kits to identify at-risk individuals who may require special and tailored interventions and/or counseling (Mar 2016) [4]A descriptive database for the prevalence of these SNPs among Filipinos (Mar

2016) [4]

INDUSTRIAL BIOTECHNOLOGY

INDUSTRIAL BIOTECH

Microbial cultures and Product Application for:a. Functional Food Ingredients (Aug 31, 2013) [20].b. Colorants & Flavoring (Mar 31, 2013) [19].

Establishment and optimization of the production of oil and protein enriched residues from pili pulp, process testing and evaluation of product quality [40].

Screening & other varietal studies, biochemical studies for :1. Plant Growth Hormone Producers/ Biofertilizers [24], [25], [26]2. Aquaculture Probiotic feeds [27].3. Protein-Rich-by-Products feeds from Enzyme Processing [28] [30].

Extraction studies, varietal studies, product dev’t & process design for1. Aquaculture Probiotic feeds [27].2. Plant Growth Hormone Producers/ Biofertilizers [24], [25], [26]

Identified strains, stable starter, cultures, applications for Indigenous Food Fermentation(April 31, 2013) [21]

Screening of strains, Optimization/Scale-up, Product Testing, Product Dev’t, Application studies, testing for Colorants & Flavoring &n Functional Food Ingredients [19], [20]

Screening, improvement of strains, prod’n process for fermentation in cacao beans [21] Screening of isolates, process dev’t , applicat’n studies for fermentat’n in cacao beans [21] Scaling up, feasibility, setting up of incubation facility (5,000L) of industrial Enzymes [23] Optimization at lab scale, stability and quality testing of Industrial Enzymes [23]

Product Testing, Product development, Application studies, testing for Microbial-based Colorants & Flavoring (Monascus purpureus M108) [23]Extraction studies, varietal studies, product development &

process design for Protein-Rich-by-Products feeds from Enzyme Processing [28] [30].

Identified strains, stable starter, cultures, targeted applications on Inoculants for fermentation in cacao beans (Dec 31, 2014) [22]

Product & processing technology

Identified strains, cultures, applict’ns on Inoculants for fermentation in cacao beans (Dec 31, 2014) [22]Preliminary Extraction & Application Studies of Industrial Enzymes (Jan 31, 2014) [23]Upscale Production of Industrial Enzymes (Feb 28, 2014) [28], (Feb 9, 2015) [30]Microbial cultures and Product Application for Functional Food Ingredients (Aug 31, 2013) [20].

Small-scale genetically engineered microbial production of lactic acid

Production / manufacturing of food ingredients, enzymes, microbial starter culture, and natural products00

Product and process technologies, 1.From Healthy sugars (xylitol from the hydrolysis products of corn cobs, corn fibers & sugarcane bagasse)

(Proposal) [41]2. From Microbial-Based Colorants and Flavorings (Feb 28, 2015) [29]

Upscale Production using conventional fermentation runs of Industrial Enzymes (Proposal) [35]Commercial products of Industrial Enzymes (Feb 9, 2015) [30]

Globally Competitive and Innovative Industry and Biotechnology Services

Product & processing technology:1. For Plant Growth Hormone Producers/ Biofertilizers (Aug 31, 2014) [24],

(June 30, 2014) [25] (June 30, 2014) [26].2. And new packaging for fermentation process inoculant in cacao beans (Dec

31, 2014) [2]3. For Protein-Rich-by-Products feeds from Enzyme Processing (Feb 28,

2014) [28]. 4. For Colorants and Flavorings (Feb 28, 2015) [29]5. For Aquaculture Probiotic f eeds(June 30, 2014) [27].6. From Pili pulp oil and by-product feed (June 30, 2014) [40].

Commercial products 1. From plant-growth hormone producers/biofertilizers (Aug 31, 2014) [24], (June 30, 2014) [25]

(June 30, 2014) [26].2. Of Innoulant for Fermentation Process in cacao beans (Dec 31, 2014) [22]3. Of Industrial Enzymes (Feb 28, 2014) [28],

Pilot-scale genetically engineered microbial production of ethanol & lactic acid for industrial consumption.

Product and Lab scale processing technology of pili resin (Proposal) [42]Large Scale Production using vectors for cry proteins as fertilizers (Proposal) [43]Formulated Bacteriocin Product, Optimized Process (Proposal) [34]Lab Scale Production Technology of Other Industrial Enzymes (Proposal) [37]Product and process technologies from Enzymes from Yacon(Proposal) [37]Commercial products pf Industrial Enzymes for Food and Beverage Applications (Proposal) [35]

Large Scale Production using vectors for cry proteins as fertilizers (Proposal) [43]Lab Scale Production Technology of Other Industrial Enzymes (Proposal) [37]Product and Lab scale processing technology of pili resin (Proposal) [42]Formulated Bacteriocins Product, Optimized Process (Proposal) [34]

Review and updatingStabilization studies, packagingAdvanced delivery studiesField testing, market testing, commercialization

Upscale processing technology of pili resin (Proposal) [42]Product Safety Sheets, Stable Products, Shelf Life Information (Feb 28, 2015) [29] , (Proposals) [31] [32] [33]

Product and process technologies 1. From Healthy sugars (xylitol from the hydrolysis products of corn cobs, corn fibers and sugarcane bagasse) (Proposal)[41]2. From Microbial-Based Colorants and Flavorings (Proposal) [31] [32] [33]3. From Enzymes from Yacon(Proposal) [37]

Stable Strains, Patent Organisms, customized staeter cultures, optimized process for starters of Indigenous Food Fermentation (Proposal) [36]Upscale Production using conventional fermentation runs of Industrial Enzymes (Proposal) [35]

INDUSTRIAL BIOTECH: Continuation… Globally Competitive and

Innovative Industry and Biotechnology Services