Biotech in Animal Production

CNG NGH SINH HC

NG DNG TRONG

CHN NUI V TH Y

PGS. TS. Dng Thanh Lim

B mn Dinh dng

Khoa Chn nui Th Y

Trng i hc Nng Lm

Cng ngh sinh hc

trong chn nui ng vt

1. Cng ngh sinh hc trong sinh sn vt nui.

2. Cng ngh sinh hc trong k thut di truyn.

3. Cng ngh sinh hc trong sn xut thc n

chn nui v thc phm.

Cng ngh sinh hc

trong sinh sn

ng vt

Bioscope II

Atomic Force MicroscopeMultiMode

scanning probe microscope

Knh hin vi quan st c phng i ln c kh nng

quan st v chp ct lp cu trc t bo

Knh hin vi

thao tc c

th ht ly

nhn v cy

chuyn

nhn t bo

Cng ngh sinh hc ng dng trong sinh

sn v tnh vt nui sn xut sinh

phm dc v thc phm chc nng

Hai trng hp nhn dng

thnh cng trn th gii

- Sinh sn v tnh cu Dolly

- Sinh sn v tnh chut Cumulina.

- Sinh sn v tnh ng vt chuyn gen

Ngi ta hy vng vi k thut ny, c th to ra

nhiu con ging sn xut dc phm trong thi

gian ngn, hoc to t bo gc cung cp cc

c quan b phn cho c th ngi.

Cu Dolly ra i bng

phng php sinh sn v tnh

Chut Cumulina ra i bng

phng php sinh sn v tnh

1. To ra ging ng vt c kh nng khng bnh tt:

Bnh b in: Trc ht cn to ra ging ng vt c kh nng chng li bnh b in BSE (bovine spongiform encephalopathy).

To ra ging chut chuyn gen nhn bit nhanh PrP Pht hin sm nhng triu chng bnh l no Chn, nhn ging n dng khng li prion (PrPR).

Bnh vim v:

Th sn xut protein sa c cha lysostaphin, mt loi protein enzyme c kh nng chng li staphylococcus, vi khun gy vim m trong v. y l hng nghin cu rt kh thi.

Nhn v tnh ging t cha bnh (Therapeutic breeds). Nhng ging ny c kh nng sn xut protein khng sinh chng khun. tng t nghin cu kh nng t khng ca trn t?

Mc tiu ca nghin cu chuyn gen, sinh sn v

tnh l to nhng ng vt c nhiu u th

To ra ging heo thi t phosphate ra mi trng.

To ging heo cho tht c cht lng tt trong ba n.

To thnh phn dinh dng sa v tht tt cho sc khe.

Xy dng ging trang tri c cht lng tt

C kh nng khng khunThay i nhng thuc tnh ch bin:

- Gim thi gian lm kt vn

- Tng sc kt vn ng cc

Sa c hot cht chng khun gy bnh

v vi khun lm cho sa mau h hng.

-10

-5

0

5

10

15

20

25

30

#34 #37 #53

SFA

MFA

PUFA

Cht bo sa thay i c li cho sc khe con ngi:Gim thp acid bo no,

Lm tng acid CLA, linoleic

bin i do vi khun d c,

chng vim, chng ung th.

Ging d sa chuyn gen

-10

-5

0

5

10

15

20

25

30

#34 #37 #53

Loi Protein dc phm Chc nng cha bnh Loi th

-1 - antitrypsin C chc nng chng vim D, Cu

Anti-thrombin III Chng s nhim trng v x cng D

nghn mch, cc mu ng.

C1 inhibitor Cha ph huyt qun do di truyn Th

Collagen Cha bng, gy xng, incon B

Factor IX & VIII Bnh a chy mu C r phi

Cu, heo

Fibrinogen cha fibrin glue, bng, phu thut, Heo, cu

trong ha tr liu, trong dc phm

Human fertility hormones Trit sn, vaccin nga thai D, b

Human hemoglobin Thay th mu ngi, truyn mu Heo

Human serum albumin Cha bng, shock, chn thng, D, b

phu thut

Lactoferrin Tr vi khun gy bnh ng rut B

LAtPA Tissue Plasminogen Activator (t-PA) Cha lot do mu tnh mch D

Monoclonal antibodies (Mab) Chng ung th kt trng D

anti-TNF IgG1 (monoclonal) Chng li c au tim D

Tissue plasminogen activator t qu, deep vein D

thrombosis, pulmonary embolism

Trang tri nui ging ng vt cng ngh sinh hc dc phm

Table modified from GEN Co.

Loi sn phm chuyn gen N sn xut Th trng. Giai on TN

h- Collagen Cattle** Pharming $500 M (+) Sng ch

h- Fibrinogen Cattle** Pharming $600 M (+) Cha ng k

h- Esterase Inhibitor **/ Cattle Rabbits Pharming $150 M Cha ng k

h- Factor VIII Pigs ProGenics* $300 M (+) Cha ng k

h- Alph- glucosidase Rabbits/ Cattle** Pharming $300 M (+) Phase II (Eur)

h- AT- III Goats GTC ? Phase II (US)

h- SA Cattle** GTC $1B Cha ng k

h- SA Cattle** Argentina Cha ng k

Spider Silk Goats** Nexia Huge Device

h- AAT Goats PPL/ Bayer ? Phase II

h- Factor IX Sheep PPL ? Cha ng k

h- Lactoferrin Cattle Pharming ? Cha chc chn

** Animals made using nuclear transfer.

Nhng nghin cu ng vt chuyn gen c nhiu tm nng

dc ng dng vo sn xut

Table modified from GEN

ng vt chuyn gen v sn lng Protein sinh phm ca n

c tnh sn lng protein sinh phm trong sa v qui m n th chuyn gen trn Th gii

Loi Protein sinh phm c tnh hng nm cn, kg c tnh qui m th Loi th

Protein C 100 33 B1,100 Heo

tPA 75 600 DCu

1-anti-trypsin 5,000 33,000 Cu42,000 D

Factor IX 2 13 Cu

22 Heo

Human serum albumin 1,000 300 B8,300 D

The number of lactating animals may be calculated as: (expression in g/L) x (annual milk

production in L) x (purification efficiency)

t-PA

BloodClot

C ch ph hy huyt khi trong c th bi sinh phm t-PA (Tissue Plasminogen Activator)

t-PA l mt enzyme, n c th ph tan huyt khi ng

lm tt nghn mch mu

PlasminogenS hot ha

Plasmin

B gySi Fibrin

Ph tan huyt khiT chc

Thng tn t-PA

StreptokinaseFrom Bacteria

UrokinaseFrom the Kidneys

To ra con d nhn gen ti t hp 1:Vector cu trc (Construction)

Murine Way Acid Protein Promoter

LAtPA cDNA

SV40 Poly A Signal

Murine Way Acid Protein Promoter, tn hiu biu l

gen trong nhng t bo thuc ng vt c v.

Qui tc sp xp LAtPA cDNA cho im bin i t tPA (asn glu AA 117) c glycosylated vi longer

acting (LA) tPA

SV40 l virus c cung cp poly A (on cui gen), tn

hiu t bo mRNA kt thc.

Promoter

sequenceGene

Poly A

sequence

Hp t

Zygote

Nucleus

Suctio

n

Suction Pipit

Vector

Micro-injector

To ra con d ti t hp 2:Vector lng ghp (Insertion)


Nucleus

Zygote Vector


Nucleus

ZygoteNhiu phin bn ca vector b b

gyMt s vo ctrong nhn


Chromosome

Nucleus Vector


Vector lng ghp vo cchromosome

Sinh sn v tnh to ra con d ti t hp 3: D con ln ln cho sa c cha tPA

Hp t ti t hp hoc

phi

Thu hoch sa

tPA tinh ch

Nhn v tnh b chuyn gen c kh nng

sn xut protein chng bnh vim vTransgenic Cow Clone for

Mastitis Disease Resistance

By Jan SuszkiwJanuary 10, 2001 BELTSVILLE, Md., Jan. 10--U.S. Department of Agriculture and University of Vermont (UV) Cc nh khoa hc trng i hc Vermont sn xut dng thun v tnh b Jersey cung cp gen chng bnh vim v cho ngnh Cng ngh sinh hc to ging b sa c kh nng chng li bnh vim v do vi khun.

T bo tuyn v b Jersey ny c kh

nng sn xut Lysostaphin, l mt loi

protein endopeptidase c hiu l h

mng t bo ca vi khun gy vim v

http://www.ars.usda.gov/is/pr/2001/010110.2.htm

Nhng con b chuyn gen ny c nhn ging v tnh, n c kh nng tng hp ra mt loi men c tc dng ph hy mng t bo vi khun gy vim v. Do vy b t bnh nn t dng khng sinh iu tr vim v, t t to ra dng vi khun khng thuc.

B Holstein bin i gen c kh nng

sn xut protein lysostaphin khng t

cu khun Staphylococcus aureus

gy bnh vim v b

Nhng con b sa sn xut sa giu

lysostaphin c gi tr nh mt loi

thc phm chc nng.

Cng ngh chuyn gen

to ra ging gia sc c

kh nng sn xut protein

c tc dng cha bnh v

phng chng bnh tt

To ra ging ng vt sn xut thucTrang tri dc phm sinh hc phn t

http://muextension.mi

ssouri.edu/xplor/regp

ubs/ncr552.htm

To ra phi t th tinh

trong ng nghim.

a gen DNA c

kh nng tng hp

ra dc phm vo

phi th tinh.

Cy phi chuyn

Gen vo cu ci cho

mang thai phi c

chuyn gen

Cu con chuyn gen

sn xut thuc tr

bnh trong sa

Nhng Protein c tc dng cha bnh trong sa

c sn xut ra bi th cho sa chuyn gen.

Sn xut ch Y khoa siu nh, siu chc, siu mnhDSpider silk

protein

Hp cht khng khun phng bnh vim v b.BLysostaphin

Cha bnh x u nang v kh thng.CuAlpha-1-

antitrypsin

Khng sinh t nhin c s dng trong phu thut tim BLactoferrin

Cha bnh x u nang (cystic fibrosis)CuCFTR (cystic fibrosis

transmembrane

conductance regulator

Cha bnh a chy mu (hemophilia)D, Heo,

Cu

Factor VIII,

Factor IX

Lm gim s lng mu cn thit trong mt s trng

hp phu thutD

Antithrombin

III

ng dng trong iu tr bnhng vtTn Protein

http://www.ext.vt.edu/pubs/biotech/443-003/443-003.html#L3

Sn xut sa b giu lactoferrin

c gi tr phng chng bnh tt.

Lactoferrin ti t hp ca ngi (rhLF), mt loi phc glucoprotein-Fe. Protein lactoferin ti t hp ngi (rhLF) c trong sa b chuyn gen ging vi lactoferrin t nhin ca ngi (hLF t nhin), u c v tr gn Fe v phng thch Fe ging nhau.

Lactoferrin c kh nng khng khun, khng nm, khng ni c t, v khng hoat ng ca cc virus. Lactoferrin tng tc vi t bo ch, iu bin tin trinh vim ty, v t bo v ca t bo ch. Lactoferrin trong sa c vai tr hp thu Fe, c th coi y l mt loi sa dc phm chc nng.

Sn xut trng g cha bnh ung th

Mt nhm cc nh khoa hc Anh

pht trin thnh cng loi g

Chuyn trng bin i gen c kh

nng ra nhng qu trng cha

cc loi protein chng ung th.

Trung tm sng to ra cu Dolly

bng sinh sn v tnh nm 1997,

lai to thnh cng khong

500 con g bin i gen c kh

nng trng chng ung th.

Loi protein cha bnh nm trong

lng trng trng, c th chit xut

sn xut thuc cha ung th c

hiu qu v tn t chi ph.

Loi trng g ny c cha protein miR24

(mt loi khng th cho php cha tr bnh

ung th da) c kh nng ngn chn s tng

sinh ca virus trong t bo. Cc nh nghin

cu vn cn phi mt khong 10 nm na

cung ng i tr cho th trng mt loi

protein chng ung th gi r.

68-nucleotide miRNA precursor, t y sn xut

ra 2 miRNA, vi 2 miRNA ny s tng hp ra

2 loi protein: smiR189 v miR24. Trong ny c

protein miR24 tn cng t bo ung th

Trng g bin i gen c kh nng cha bnh ung th

Chuyn gen tng hp protein ca loi

nhn vo trong gen tng hp sa ca loi

d ni, n sn xut ra mt loi protein

sa c bit c th ko ra si t lm ch

khu Y khoa, tan c trong c th.

D sa Biosteel ca Cng ty

Nexia Biotechnologies In C.

Qubec, Canada.

Sn phm ch yu ca Cng ty Nexia ca Canada l dc phm

v cht liu sinh hc. Protein ti t hp trong sa d BELE c

c tnh ging nh si t ca nhn ni. V si nh, chc nh thp

nn ngi ta cn gi sn phm l BioSteel, Vi phng php

ny Cng ty Nexia sn xut trn 80 loi sinh phm dc.

Chuyn on gen

nhn ni vo dGing d cho

Sa ko si

D BELE s dng trong nghin cu

chuyn gen ca Cng ty Nexiabiotech.

D BELE c c im l thnh thc rt sm, do

rt thun tin trong cng tc nghin cu di truyn.

Sn xut sa

Sn xut sa

Mang thai

Gieo tinh

Thnh phn Amino Acids

trong sa d chuyn gen

1. Glycine2. Alanine 3. Valine4. Leucine5. Isoleucine6. Serine 7. Threonine 8. Phenylalanine 9. Tyrosine 10.Tryptophan

1. Aspartate 2. Glutamate 3. Asparagine 4. Glutamine 5. Cysteine 6. Methionine 7. Lysine 8. Arginine 9. Histidine 10.Proline

Khng khc g khi so snh vi protein truyn thng

Sa d chuyn gen ca nhn ni c s dng

lm ch khu tan, siu chc dng trong Y khoa

Ch khu Y khoa rt cht, c th cu c ln c

Th nghim ch khu Y khoa trong phng TN

Kt qu nhng th nghim ch khu

Y khoa lm t sa d nhn gen nhn ni

Nhm cc nh khoa hc tho lun

cc tng ng dng cng ngh mi ny

Cng ngh sinh hc ng dng trong s sinh sn Th tinh nhn to trong ng nghim

Chuyn cy phi, nhn ging qu

http://www.scienceatstmarys.net/KS3 computer work/Variation/Selecting the best IV.ppt

Chuyn cy phi iu tr him mun

Thit b knh hin vi thao tc

s dng trong cng ngh sinh hc

Micromanipulation

http://www.cvm.tamu.edu/CollegeHour/02 Spring 2005/08 Reproductive Sciences.ppt

Knh hin vi thao tc ly nhn v bm nhn t bo

Thao tc vi tim di

knh hin vi thao tc c phng

i ln chuyn nhn t bo

Chuyn nhn t bo

Nucleus Transfer

Khot ly nhn t bo

(removing the nucleus)

Bm nhn vo trong t bo

Chuyn nhn t bo

Nucleus Transfer

a tinh trng vo bn trong t bo trng

Sperm Injection

Tim tinh trng vo trong

t bo cht ca trng, nui

trng trong ng nghim

thnh phi v cy vo t

cung

K thut ny p dng cho

sinh sn th qu him, n

ng v sinh khng c kh

nng th tinh

http://ims.cos.edu:8081/Jim Sellers/Bio 20 PP-Frontiers in Biology Fall 2005/Weathers_Amanda.ppt

Giao tTrng Tinh trng

Tin nhn

cTin nhn

ci

Vi pipet

Tim gen

Chuyn gen

Trng

th tinh

Gen tim vo trng

Th tinh bng phng php vi tim

Ci thin ngun gen

Kim tra nh gi

Ph bin sn xut

Cng ngh sinh hc c th rt ngn m hnh

nhn ging, khng cn tri nhn ging b m?

Ging

Ht nhn

Ging thng phm

Thp ging Phn bit gii tnh, nhn v tnh

AI, MOET, JIVET

p dng cho ging cao sn, nhng kh nng

sinh sn, nhn ging t nhin thp.

Artificial insemination (AI)

In Vitro Fertilisation (JIVET)

Embryo transfer (MOET)

Tng nhanh i sau t mt gia nh

Pht huy c tnh tt t gc cha m tt chn la k lng, tn km

Nhn nhanh c th c c tnh tt t gc cha m phc v sn xut.

http://centaur.vri.cz/docs/CENTAUR_3.PPT

http://www.cvmbs.colostate.edu/bms/640_RM_endangsld.ppt

TLHFSH

GnRH

T = Testosterone

LH = Leuteinizing Hormone (regulates T secretion)

FSH = Follicle Stimulating Hormone (regulates seminiferous tubule function)

GnRH = Gonadotropin Releasing Hormone (regulates LH & FSH secretion)

INHIBIN from Sertoli cells feedsback on the pituitary to regulate FSH secretion

PITUITARY/TESTIS

FEEDBACK SYSTEM

TIME

CO

NC

EN

TR

AT

ION

INHIBIN

http://www.umanitoba.ca/womens_health/rp017c.ppt

http://depts.washington.edu/cfas/WHIN/2005_sanjuan/Bardeguez.ppt

HYPOTHALAMUS

GnRH (Gonadotropin releasing hormone)

PITUITARY

LH (Luteinizing hormone)

FSH (Follicle stinulating hormone)

TESTIS

Leydig cells (produce Testosterone)

Sertoli cells (produce Inhibin)

Nhng hormon s dng trong

k thut sinh sn nhn to

Cng ngh sinh hc ng dng

trong sn xut hormon

1. Sn xut hormon ti t hp: rBST, rBGH

2. Sn xut Hormon IGF iu tr bnh ln.

3. Sn xut Insulin ti t hp: rInsulin

Cng ngh sinh

hc sn xut

Insulin

Nguyn l:- Ly on plasmid tng hp insulin trong t bo ngi chuyn vo plasmid E. Coli

- E.Coli tng hp ra Insulin

ng dng:- Thuc tr tiu ng- Lm vaccine ch khng huyt thanh insulin.

- Chn on bnh

http://www.idrc.ca/uploads/user-S/11095853171RabihTalhouk-AsPresented-IDRC-Sep29-04.ppt

Cu trc ca Insulin

C 2 chui polypeptide; chui th nht c 21

acid amin v chui th hai c 30 acid amin.

Hai chui lin kt vi nhau qua cu disulfide

Gene m ha protein insulin tim thy trong

chromosome 11, da vo cu trc ca gen

ny, ngi ta ti t hp DNA tng hp insulin

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Cu trc ca Insulin

http://www.fda.gov/cder/meeting/followOn/Ahn.ppt

K thut ti t hp DNA

Nhng Enzyme gii

hn c s dng

ct gen insulin v ct

plasmid ca vi khun

(E. coli) cng ging

nhau u cui nn

dnh li c sticky

ends

K thut ti t hp DNA

Dng bin i ca E. coli c s dng

trnh s tn cng gen l (foreign genes)

Gen insulin gn vo E. coli l

B-galactosidase, n kim sot s sao chp.

Nhng t bo vi khun s ti to v sao

chp bn sao ca gen insulin.

K thut ti t hp DNA

Protein Insulin c tinh ch

(B-galactosidase thc hin)

Nhng chui peptide hn hp v ni li vi

nhau bi cu disulfide.

Sn phm cui cng c tn goi l Humulin

tn ha hoc ni ln insulin ca ngi.

S dng insulin ngi chng

bnh tiu ng

Trn th gii c hn 150.000.000 ngi mc chng bnh tiu ng. Hu qu ca n rt nghim trng, gy ra nhng bin chng phc tp,nguy him n tnh mng.

Tng chi ph cho cha tr bnh tiu ng ch ring cho nc Php c n 5,7 t Euro trong nm trong 2002

Insulin ti t hp ln u tin xut hin trn th trng nm 1982

Nhng dng sn phm insulin pht trin nhanh chng v a dng (insulin tc dng nhanh- hoc tc dng ko di, hoc insulin dng ht)

Trong nm 2002 tng s bn ra l 4.2 t Euro

Bin chng c th xy ra khi

s dng Insulin ngi

Hypoglycemia (thp ng huyt) xy ra vi

insulin ngi nhiu hn so vi insulin ng vt.

S dng insulin h ng huyt ngi tiu

ng nn c s khm bnh v hng dn ca

Bc s iu tr. Khng nn t tin dng rt nguy

him.

Sn xut bovin somatotropin (bST)http://www.monsantodairy.com/about/history/

Qu trinh sn xut

somatotropin v

ng dng trong

chn nui b sa,

nng nao nng

sut, cht lng sa

Sn xut bST bng con ng cng ngh sinh hc

Ti t hp sn xut bST v ng dng nng cao nng sut sahttp://www.extension.umn.edu/distribution/livestocksystems/DI6337.html

Hot ng ca BST t nhin

Sn xut BST tng hp

1.Thy trc tuyn yn tit ra BST, l

mt hormon c cu trc nh 1 peptid,

c tc dng ln s tng hp sa.

2.Hormon ny vo mu i khp c th.

3.BST tc ng n gan sn xut ra mt

protein quan trong IGF-1 (Insulin like

Growth Factor-1).

4.IGF-1 tc ng ln tuyn v, phn ct

cht bo sinh nng lng cho sn xut sa..

A.Gen p ng cho sn xut BST c

c lp t DNA ca b. B.Gen ny c

a vo vi khun E. Coli bng k thut

c bit. C. Vi khun E.Coli c gen tng

hp BST c nhn ln trong mi trng

nui cy. D.Thu nhn BST trong mi trng

E.Tinh loc BST t VK to ra BST tng hp.

http://www.avs.uidaho.edu/avs221-bio212/Past Team Presentations/Recombinant Bovine Somatotropin.ppt

B sa c chch

Kch t ti t hp rbST

B c chch

hormon BST

c g c bit

trong nui

dng khng?

C, l nhu cu

dinh dng cao

hn, nhng tiu

hao dinh dng

cho sn xut1 kg

sa thp hn b

bnh thng.

Cc vn rc ri

c lin quan vi rBGH hay rBST

Lm tng 16 vn th y xy ra trn b sa dng rBST: Gim t l u thai v trong lng s sinh ca b,

Tng t l tiu chy,

H hi chn,

Thng tn t bo somatic (c m),

Gy vim v.

Vim v v khng thuc lin quan n sc khe ngi

C s lin quan gia IGF-1 v bnh ung th trn ngi

trn b sa c s dng rBST.

CHA BN CI

IGF-1 hin din v ging nhau trn b v ngi

rBGH lm tng IGF-1 trong sa b

IGF-1 tng cao kch thch t bo ung th trn ngi

rBST, IGF-1 v UNG TH

rBGH

Kch thch ung th

trn ngi

IGF-1 tiu ha nh th no?!

Tng IGF-1

Trong sa b

IGF-1 tng

trn ngi

CASEIN BO V IGF-1

Casein nh hng trn ton b cu trc IGF-1 (80%) v receptor kt dnh hot tnh. . .Ngun ti liu: (C.J. Xian et al, Degradation of IGF-1 in the adult rat gastrointestinal tract is limited by a specific antiserum or the dietary protein casein, Journal of Endocrinology, v. 146, 1995.)

T bo ny cho thy IGF-1 c th tiu ha cn st li (67%) khi c s hin din ca casein.Ngun ti liu: (Michael Hansen, Consumer Policy Institute, Letter to Maine Attorney General G. Steven Rowe, Feb. 11, 2003, citing T. Kimura et al, Journal of Pharmacology and Experimental Therapeutics, v. 283, 1997.))

Casein tng cng cao, cng lm cho IGF-1 tr nn n nh hn, kh b men tiu ha phn giiNgun ti liu: (P. Anderle et al, In Vitro Assessment of Intestinal IGF-1 Stability, Journal of Pharmaceutical Sciences, v. 91, 2002.)

S dng GH iu tr bnh lnng dng trong iu tr bnh ln bm sinh.

http://arbl.cvmbs.colostate.edu/hbooks/pathphys/endocrine/hypopit/gh.html

(Insulin like

grower factor)

Somatotropin c an ton thc phm

cho ngi tiu dng khng?

L mt Protein hormon

1. C cc m vi s lng nht nh.

2. Khng tch ly trong m m.

3. L mt protein c cu to t cc acid amin.

4. Gii hn tc ng theo loi.

5. Khng c hot tnh nu s dng qua ng ming, v n b tiu ha nh nhng protein khc trong thc n

L mt Steroid hormon

C th tch ly trong c th ng vt v ngi.

C nhiu trong cc m m, kh loi ra ngoi.

L phn t hu c c ngun gc cholesterol.

Khng gii hn tc ng theo loi, k c ngi.

C hot tnh khng i khi s dng qua ng ming.

Posilac l sn phm thng mi

c cha rBST c s dng M

Posilac c sn xut bi cng ty Monsanto

Ch c FDA chp nhn cho s dng nng cao

sc sn xut sa ca b.

L ch phm s dng chch vo c bp

9 triu b sa cc nc th gii th ba s

dng ch phm Posilac ca Monsanto.

Nhng

Canada CHA (Canadas health

administration) khng chp nhn cho s dng

Posilac

EU (European Union) cm s dng rbST

Nht bn, c v New Zealand cm s dng

Posilac

Mt s kch t tng hp thuc steroid

b cm s dng trn th v VN.

S TT Tn ho cht, kch t S TT Tn ha cht, kch t

1 Carbuterol 9 Metronidazole

2 Cimaterol 10 19 Nor-testosterone

3 Clenbuterol 11 Ractopamine

4 Diethylstilbestrol (DES) 12 Salbutamol

5 Dimetridazole 13 Terbutaline

6 Fenoterol 14 Stilbenes

7 Isoxuprin 15 Terbolone

8 Methyl-testosterone 16 Zeranol

Nhng loi kch t Steroid hormon b cm s dng

OH

H

H

H

CH3OH

O

CH3

H

H

H

CH3OH

O

CH3

H

H

H

CH3C

CH3

O

Beta-estradiol TestosteroneProgesterone

O

H

CH3OH

H

CH3

CH3

OH

OHO

OH

CH3HOOH

OHHTrenbolone Zeranol Diethylstibestrol

NH2

Cl

Cl

CH CH2NH C(CH3)3OH

Clenbuterol

OH

HOH2C

CH CH2NH C(CH3)3OH

Salbutamol

H2N

F3C

CH CH2NH C(CH3)3OH

ClMabuterol

H2N

NC

CH CH2NH C(CH3)3OH

Cimaterol

Cng ngh sinh hc vi sinh

ng dng sn xut thc n b sung

1. Thng qua t bo nm men sn xut

Selenium hu c ng dng trong thc n

chn nui v phng bnh thoi ha c.

2. S dng vi khun hu ch ng rut

sn xut Probiotic phng chng bnh

ng rut thay th khng sinh.

Selenium

Vai tr sinh hc ca Se: Phng chng nhng bnh c lin quan vi thiu Se nh:

Bnh teo c trn cu, b v g (thng xy ra trn th nui theo li CN) Bnh hoi t gan trn chut v heo do thiu Se Bnh th tng tit dch trn g v g Ty (tch nc xong bng) Bnh a thoi ha hoi t tim, gan, c v thn trn chut.

Chc nng trong trao i cht ca Se: Selenium cu to enzyme glutathione peroxidase, ph hy cc gc t do peroxides trong cytosol, gc ny c nguy c gy ung th.

C th coi Se c hot tnh chng oxyha (vi vy c mi quan h vi vitamin E trong hot ng chng oxyha).

Mui selenium v c tng i c, selenium hu c c nhiu trong t bo nm men khng c, s dng an ton hn. V vy cng ngh sinh hoc tng hp selen hu c.

Selenium tham gia trong enzyme Glutathione Oxidation thc hin

chu trnh phn ng oxyha kh, bin H2O2 thnh H2O, chng li

s hnh thnh cc gc t do trong c th http://lpi.oregonstate.edu/infocenter/minerals/selenium/gsh.html

Selenium hu c v chc nng sinh hc

Cng ngh sinh hc sn xut

Selenium hu c bi

t bo nm men

Video clips: 1, 2.

Cng ngh sinh hc ng dng trong sn

xut Probiotics, Prebiotic v Synbiotics

Probiotics = L nhng vi sinh vt hu ch, sng c trong mi trng ng rut, ci thin sc khe ng rut vt ch. Tm kim VSV probiotic trong ng rut vt ch khe mnh, nh danh, nui thun hoc hn hp.

Prebiotics = Ngun thc n cho vi sinh vt sng hu ch trong ng rut vt ch (khng phi cho vt ch). Prebiotics thng l cc cht x tan khng tiu ha on trn ca rut, c kh nng ln men su\inh acid hu c bi VSV probiotics.

Synbiotics = Hn hp probiotics v prebiotics c tc dng:Ci thin kh nng sng v chim ng trong ng rut caprobiotics. Cho nhiu nh hng tt hn s dng probiotics n thun. Prebiotics bao bc xung quanh VSV probiotics.

C ch hot ng ca

Probiotics, prebiotic v synbiotics

(S khng chim lnh hay s cnh tranh loi b)

1. Sn xut cc cht khng vi khun gy bnh.

2. Gi cn bng pH thp bi nhng acid bo mch ngn v acid lactic do vi khun hu dng ln men sinh ra.

3. Nng cao hot ng ca i bch cu, thc bo VK gy bnh

4. c ch cnh tranh v tr bm dnh ca vi khun gy bnh.

5. Cnh tranh ngun dinh dng vi vi khun gy bnh.

6. iu chnh khng th, tng cng khng th khng c hiu.

7. Gim thp s chuyn i yu t tin ung th thnh ung th bi nh hng ca hot ng enzyme.

8. Gim chng to bn, t gim c t sinh ra trong rut.

9. Gim cholesterol ti hp thu, t gim cholesterol mu.

89

Thnh rut

T bo nim mc rut

VK c li (Probiotics)

VK gy bnh

A.

B.VK gy bnh

VK c li (Probiotics)

Thnh rut

T bo nim mc rut

Hng ro phng ng t nhin

90

Bacillus subtilis/cereus/mesentericus

Clostridium butyricum, Bifidobacterium

Lactobacillus acidophilus

Enterococcus faecium..

S DNG VI KHUN C CH

TR SINH (PROBIOTIC)

Saccharomyces cerevisiae/boulardii..

Aspergillus oryzae

c im chung ca

probiotics l:

Tng i thch nghi trong ng rut ng vt

C kh nng chng li stress trong d dy, rut

khng li vi cc yu t ch bin thc n

Phi t cc yu cu, qui nh ca lut l an

ton sinh hoc v c hiu qu ln sc khe.

Ny chi trong ng rut

hiu qu hng ro sinh hc

Tng cng ti hp thu Nit

Sn xut cc enzyme

Sn xut cc acid hu c

Kch thch min dch cc b

1

Enzymes

2

N

3

5

acid hu c4

C ch tc ng cu

Probiotic

Video clips 1, 2

T l tiu ha hi trng (Ileal)

G 21 ngy tui

+15.9%

+18.9%

+17.9%

+3.8%

+3.5%

+7.9%

+12.8%

+12.2%

+15.1%

70

75

80

85

90

95

100

MET LYS ARG THR VAL LEU ILE PHE HIS

Essential Amino Acids

CONTROL ESPORAFEED

50%

60%

CaCONTROL ESPORAFEED

50%

60%

Mg

CONTROL ESPORAFEED

10%

15%

20%

K

CONTROL ESPORAFEED

Ca

K

Mg

nh hng dng tnh ca Probiotics

trn s tch ly khong

+9.1%

+12.0%

+28.6%

S QUI TRNH SN

XUT PROBIOTICS

Nhn ging a vo b Ly tm tch Sn phm th

trong ng ln men ln lp t bo t cui cng

nghim Sy phun loc

bao sp, hoc

cht x tan

New!

S QUI TRNH SN

XUT PROBIOTICS (TT)

Sn phm th

(do Norel sn xut)

Sn phm hot ng ng gi Sn phm

thng mi

Cht mang

New!

Sn xut trng g giu khng th phng chng

bnh ng rut cho ngi v ng vt

Trng g giu khng th phng v chng

bnh do vi khun v virus gy bnh

Vi khun gy bnh tn cng t bo nim mc rut v

gy mt nhiu nc, tiu chy, gim hp thu.

Vi khun bnh b khng th c hiu trong lng

kt dnh v lm v hot chng

Virus gy bnh tn cng nim mc rut

l cho t bo nim mc v tung, gy tiu chy

n lng trng c khng th c hiu s lm

v hot cc virus gy bnh ng rut

Trng g giu khng th chng bnh c

sn xut Nht Bn bi Cng ty GHENhttp://www.ghen.co.jp/en/ingredients/index.html

ELISA

QUY TRNH SN XUTPROTIMAX

PROTIMAX

ProtimaxProtimax

G mi nui trong tri SPFChng vaccin c kim Sot g sn xut khng Th lng cao

Trng g giu khng th c kim tra hm lngkhng th cn thn

Nghin cu to ra ch phm p dngVo sn xut

Th nghim khNng phng hTrn heo con

Cc loi khng th c hiu c

sn xut t lng trng g

Salmonella typhimurium

Salmonella cholaraesuis

Salmonella enteriditus

Salmonella dublin

Salmonella heidleberg

lostridium perfringens

Rotavirus

TGE

E.coli 987P

E.coli F41

E.coli K88

E.coli K99

E.coli 1362

E.coli 2134* ( 2134 is an 18

E. Coli)

E.coli 263

E.coli J5

C ch tc ng ca Protimax

PROTIMAX cn c kh nng

kt dnh cc vi khun gy bnh

v virus, khng cho chng bm

vo thnh rut v nhn ln

trong ng tiu ha ca heo

con, nh vy gip cho heo con

trnh c cc bnh nhim

trng gy tiu chy.

IgG

IgG

Nhng kt qu th nghim Protimax M

1.441.39Ch s bin chuyn thc n, FCR

+ 13.64%1.351.19Lng thc n tiu th bnh quan,

/heo/ngay (lb.)

5548.4Tng lng thc n tiu th / heo (lb.)

+ 9.46%0.940.86Bnh qun tng trng / ngy/heo, (lb.)

40.740.7S ngy nui

38.234.9Tng trng bnh qun / heo, (lb.).

51.747.4Trng lng bnh qun u ra(,lb.)

13.512.5Trng lng bnh qun u vo,( lb.)

- 29.92%1.462.09%T l t vong

4563S heo cht

30312955S heo u ra

30763018S heo u vo

% Khc bitProtimaxi chng

10.39%1.381.54Ch s bin chuyn T, FCR

- 9.40%1.061.17Lng thc n bnh qun /

heo/ngy (lb.)

46.953.1Tng thc n / heo (lb.)

+1.31%0.770.76Tng trng/ ngy/con,lb.

4445S ngy nui

34.134.6Tng tng trng / heo,(lb.)

45.145.6P bnh qun u ra, (lb.)

1111P bnh qun u vo, (lb.)

- 45.50%4.728.66%T l t vong

72131S heo cht

14551382S heo u ra

15271513S heo u vo

% Khc bitProtimaxi chng

Hiu qu s dng Protimax trn heo min Nam Carolina.

Thank you for

Your attention

Biotech in Animal Production

Documents