Illumina’s TruSight Tumor 170 is an enrichment-based, targeted panel NGS assay, optimized to detect 170 gene variants from common solid tumors. It simultaneously analyzes DNA and RNA extracted from formalin-fixed, paraffin-embedded (FFPE) cancer tissue samples or from cell lines. The method is automated on the Biomek NX P Span-8 Liquid Handler and has been Illumina Qualified. The Biomek automated TruSight Tumor 170 workflow (Fig. 1) aligns with the kit’s protocol and guarantees at least 16 RNA libraries and 16 DNA libraries can be prepared from a single reagent kit. It reduces hands-on time (Tables 1 and 2) while simplifying library prep workflow. The method interface makes setup easy: use the Method Options Selector (Fig. 2) to choose run options (e.g., number of samples), and Guided Labware Setup (Fig. 3) with automatic reagent calculation for accurate system prep that virtually eliminates setup errors. Biomek NX P Span-8 Automated TruSight Tumor 170 • Simultaneously process up to 32 samples (16 RNA and 16 DNA) • Flexibility to select modules to run and number of samples to process (3 – 32) • Modular process preserves reagents and automates most laborious steps • Proven to deliver quality sequencing libraries revealing known variants 1 Biomek Automated TruSight Tumor 170 Assay - Illumina Qualified
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Illumina’s TruSight Tumor 170 is an enrichment-based, targeted panel NGS assay, optimized to detect 170 gene variants from
common solid tumors. It simultaneously analyzes DNA and RNA extracted from formalin-fixed, paraffin-embedded (FFPE) cancer
tissue samples or from cell lines. The method is automated on the Biomek NXP Span-8 Liquid Handler and has been Illumina
Qualified. The Biomek automated TruSight Tumor 170 workflow (Fig. 1) aligns with the kit’s protocol and guarantees at least 16 RNA
libraries and 16 DNA libraries can be prepared from a single reagent kit. It reduces hands-on time (Tables 1 and 2) while simplifying
library prep workflow. The method interface makes setup easy: use the Method Options Selector (Fig. 2) to choose run options
(e.g., number of samples), and Guided Labware Setup (Fig. 3) with automatic reagent calculation for accurate system prep that
virtually eliminates setup errors.
Biomek NXP Span-8 Automated TruSight Tumor 170
• Simultaneously process up to 32 samples (16 RNA and 16 DNA)
• Flexibility to select modules to run and number of samples to process (3 – 32)
• Modular process preserves reagents and automates most laborious steps
• Proven to deliver quality sequencing libraries revealing known variants1
For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com
AAG-3266FLY12.17
Discovery In Motion | 3
Compact Bench-Top System
Core Components:
Beckman
Biomek NXP Span 8 TruSight Tumor 170 Sample Prep System
Description
BIOMEK NXP SPAN 8 WITH GRIPPER
BIOMEK NXP HALF TRASH ALP KIT
KIT, 1 ML SYRINGE - 8 PACK
Low Volume Disposable Mandrel Kit, 8-Pack
Biomek Software 4.1
NXP 3X3 ALP
BIOMEK NXP SPAN 8 ACTIVE WASH ALP KT
ORBITAL SHAKER ALP, Large Diameter Orbital Shaker Pins
Static Peltier
3rd Party Hardware
Inheco 3200376 Adapter C ABgene AB-0765, AB-0859 (PN 3200376)
Alpaqua Magnum™ EX Universal Magnet Plate (PN A000380)
Illumina
TruSight Tumor 170 Kit, for Use with NextSeq 500/550 (OP-101-1003, OP-101-1004)
Simple Setup, Flexible Run-Time Options
Figure 2. Automated TruSight Tumor 170 Method Options Selector (MOS). Provides the flexibility to select runtime options with built-in Illumina defined safe stop points.
Figure 3. Automated TruSight Tumor 170 Guided Labware Setup Interface (GLS) Reduces Setup Errors by Guiding the User through the Instrument and Reagent Setup.
Figure 4. Guided Labware Setup includes a reagent calculator with instructions for preparation and placement of each on the system.
Simple Setup Reduces Setup Errors
Contact Beckman Coulter at 1-800-742-2345Learn more
Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions