Top Banner
Bioluminescenc e: Natural Energy Provider Kenny J. Colón Research Proposal
12
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Page 1: Bioluminescence

Bioluminescence:Natural Energy

Provider

Kenny J. ColónResearch Proposal

Page 2: Bioluminescence

Introduction

Bioluminescence • The emission of non-heated

light produced by living organisms because of luciferin –reacts to oxygen to produce light- and luciferase– speeds up the process. The oxygen is used to break organic material releasing CO2 and light.

• It occurs mostly in deep-sea organisms, but also in some fungi, bacteria and insects.

Photosynthesis• The transformation of light

energy and CO2 to make organic material and liberate oxygen.

• The process starts in the stroma and in the thylakoids of the chloroplasts of a cell of a plant or other autotrophs.

• The cycle ends when a molecule of G3P (triose phosphate) transforms and produces a sugar.

Page 3: Bioluminescence

Process of Bioluminescence

The reaction involves the following elements:– Enzymes (Luciferase) - biological catalysts that accelerate and control

the rate of chemical reactions in cells.– Photons - packs of light energy.– ATP - adenosine triphosphate, the energy storing molecule of all living

organisms.– Substrate (Luciferin) - a specific molecule that undergoes a chemical

charge when affixed by an enzyme.– Oxygen - as a catalyst

A simplified formula of the bioluminescent reaction:ATP(energy) + Luciferin (substrate)+ Luciferase(enzyme) + O2(oxidizer) = light (photons)

Page 4: Bioluminescence

Abstract

• Present evidence of energy transferring from bioluminescent organisms to any other photosynthetic source.

• Develop new ways of using chemical based energy without further mayor consequences.

• Obtaining, observing and studying the interactions and behaviors between samples of bioluminescent and photosynthetic organisms.

Page 5: Bioluminescence

Hypothesis

• Demonstrate that the light emitted from a bioluminescent organism is absorbed and processed normally by a photosynthetic organism.

• Prove that the inverse processes of bioluminescence and photosynthesis can occur on a same organism not related with both adaptations at once.

Page 6: Bioluminescence

Aims

• Manage to integrate two relative processes in spite their inverse tendencies.

• Deepen the study of the bioluminescent and photosynthetic nature.

• Understand the behavior of these organisms in different stages and phases.

• Notice differences in Dinoflagellates that are exposed to bioluminescent emissions and the ones that are not.

Page 7: Bioluminescence

BackgroundBioluminescence is an adaptation that some organisms obtain from the absence of light in their environment. These creatures had to develop ways to adjust their process of producing energy.

Puerto Rico has the three most brilliant “bio bays” of the five that there are in the world. This island has enough ecosystems with bioluminescent presence to study the diversity it hides in comparison.

Page 8: Bioluminescence

Methodology

• Visit various “bio bays” and take samples of water with bioluminescent and non-bioluminescent or photosynthetic presence (Dinoflagellates).

• Gather the samples into different environments and observe which adaptation is dominant over the other, jointed and separated respectively.

Page 9: Bioluminescence

• Positive Control:– Bioluminescent and non-bioluminescent samples

together.• Negative Control:– Both samples observed separately.

Each group will be analyzed in three different areas depending on the light exposure:

• Complete dark• Outside dark exposure• Intermittent daylight exposure

Page 10: Bioluminescence

Expected Results

• The expected results are that the photosynthetic source will obtain enough light energy from the bioluminescent source and would be able to use that energy to synthesize their organic material.

• The bioluminescent source in turn will supplement itself with the oxygen liberated in the photosynthetic process of the non-bioluminescent organisms.

Page 11: Bioluminescence

Future Studies

• Deepen the study of luciferin and lucferase components to prove that it can be manipulated to non-bioluminescent organisms.

Page 12: Bioluminescence