DNA Nanotechnology Dr. Tai-ping Sun Biology 280S: Biotechnology & Genetic Engineering Joshua Mendoza-Elias Fall 2008 QuickTime™ and a TIFF (Uncompressed) decom are needed to see this In Cancer Therapeutics QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture.
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DNA Nanotechnology
Dr. Tai-ping SunBiology 280S: Biotechnology & Genetic Engineering
Joshua Mendoza-EliasFall 2008
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.In Cancer Therapeutics
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
OutlinePart 1 - The Fight Against Cancer
I. Cancer: Barriers to therapeuticsII. Cancer: Current Diagnostics & TherapeuticsIII. Definition of Nanotechnology
A. What it is isB. How it is made
V. Examples of early Nanotechnology
Part 2 - DNA as new NanotechnologyVI. Properties of DNAVII. Innovative uses of DNA-folding Pathways VIII. DiagnosticsIX. Drug releaseX. The Shape of Things to Come
CancerClinical Manifestation:
-Uncontrolled cell proliferation-Loss of apoptosis-Growth Factors/Anti-Growth Factors-Angiogenesis-Invasion/metatases
Damage to somatic cell genome:-Oncogenes-Tumor suppressors-DNA replication errors-Epigenetics
Cancer: Barriers to Treatment. Immune System Surveillance
NCI Alliance for Nanotechnology in Cancer:http://youtube.com/watch?v=5jqQxuVncmc
Outline:Part 1 - The Fight Against Cancer
I. Cancer: Barriers to therapeuticsII. Cancer: Current Diagnostics & TherapeuticsIII. Definition of Nanotechnology
A. What it is isB. How it is made
V. Examples of early Nanotechnology
Part 2 - DNA as new NanotechnologyVI. Properties of DNAVII. Innovative uses of DNA-folding Pathways VIII. DiagnosticsIX. Drug releaseX. The Shape of Things to Come
DNA Nanotechnology: PropertiesVersatile: Information on multiple levels
Nucleic acid sequence
2° & 3 ° Structure
State Dependent: f(x):Energy Environment
H = E + PV
Designed DNA molecules: Principles and Applications of Nanotechnology
Condon, Anne Nature Reviews (2006) 7: 565-575
Designed DNA molecules: Principles and Applications of Nanotechnology
Condon, Anne Nature Reviews (2006) 7: 565-575
An Autonomous Molecular Computer for Logical Control of Gene Expression
Yaakov Benenson, Binyamin Gil, Uri Ben-Dor, Rivka Adar & Ehud Shapiro Nature (2004) 429: 423-429Prostate Cancer:
PPAP2BLipid phosphate phosphohydrolase (Vascular endothelial growth factor and type I collagen inducible protein) (VCIP)
GSTP1Glutathione S-transferase. Role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione.
PIM1ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
HPNHepsin, a Putative Cell-Surface Serine Protease, is Required for Mammalian Cell Growth
References:[1] Condon, Anne. Designed DNA molecules: principles and applications of molecular nanotechnology. Nature Reviews (2006) 7: 565-575.
[2] Ferrari, Mauro. Cancer nanotechnology: oppurtunities and challenges. Nature Reviews (2005) 5: 161-171.
[3] Beneson, Y., Gil, B., Ben-Dor, U., Rivka, A. and Shapiro, E. An autonomous molecular computer for logical control of gene expression. Nature (2004) 429: 423-429.
[4] Beneson, Y., Gil, B., Ben-Dor, U., Rivka, A. and Shapiro, E. An autonomous molecular computer for logical control of gene expression. Nature (2001) 414: 430-434.
[5] Lu, Y., Liu, J. Functionjla DNA nanotechnology: emerging applications of DNAzymes and aptambers. Current Opinion in Biotechnology (2006) 17: 580-588.
[6] Dittmer, W., Reuter, A., Simmel, F. A DNA-based machine that can cyclically bind and release thrombin. Angewandte Chemistry International Edition (2004) 43: 3549-3553.