BIOLOGICAL BARRIERS...BIOLOGICAL BARRIERS 16-21 February 2014 3 Non Cellular Barriers: Mucus and Surfactant Session Chairs: Ulrich F. Schäfer and Catherine Nordgård 13:40 Mucus and

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS

16-21 February 2014

Helmholtz-Institute for Pharmaceutical Research Saarland

and

Saarland University

www.uni-saarland.de/biobarriers2014

Programme Chairs and Organisers:

Prof. Dr. Claus-Michael Lehr apl. Prof. Dr. Ulrich F. Schäfer Prof. Dr. Marc Schneider Dr. Nicole Daum Dr. Brigitta Loretz Dr. Maike Windbergs

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

1

Sunday Feb 16, 2014 Conference Start: 12:30 p.m.

Nanomedicine

10:00 Registration Open

Welcome Brunch Buffet

12:45 Opening of the Conference by the Programme Chairs and Organisers

Session Chairs: Claus-Michael Lehr and David Grainger

13:00 Hot Topic in Nanomedicine: How to Overcome Resistance to Treatments? Patrick Couvreur (University of Paris-Sud, FR)

13:30 Development of a Highly Elastic Photocrosslinkable Cell-laden Tropoelastin-based Hydrogel Nasim Annabi (MIT, US)

14:00 Crossing Biological Barriers Promoted by Cyclodextrins Gerhard Wenz (Saarland University, DE)

14:30 - 15:00 Coffee Break

Session Chairs: Maike Windbergs and Gerhard Wenz

15:00 Microfluidics for Encapsulation and Controlled Release David A. Weitz (Harvard University, US)

15:30 Physico-Chemical Concepts for Nanoparticle Transport and Penetration Through Oral Barriers David Grainger (University of Utah, US)

16:00 Porous Healing PLGA Particles for Delivery of Large Molecules Steven Schwendemann (University of Michigan, US)

16:30 - 17:00 Coffee Break

Session Chairs: Ulrich F. Schäfer and Steven Schwendemann

17:00 Bio Meets Nano: The Nanoparticle-Protein Corona Roland Stauber (University of Mainz, DE)

17:30 It Takes Two to Tango: Interactions Between Nanomaterials and the Immune System Bengt Fadeel (Karolinska Institute, SE)

Contributed Talks:

18:00 Small-Angle X-ray Scattering for Nanomedicine Andreas Thünemann (Federal Institute for Materials Research and Testing, DE)

18:15 Active Particulate Polymer Carriers Christian Wischke (HZG, DE)

18:30 Welcome Reception with Dinner Buffet

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

2

Monday Feb 17, 2014Conference Start: 8:15 a.m.

Bacterial Barriers

8:15 Welcome Address by Dirk Heinz, Scientific Director of Helmholtz Centre for Infection Research (HZI)

Session Chairs: Brigitta Loretz and Hanne Mørck Nielsen

8:30 Polymers at Bacterial and Eukaryotic Cellular Surfaces Cameron Alexander (University of Nottingham, UK)

9:00 Transport Phenomena at the Bacterial Cell Wall Mathias Winterhalter (University of Bremen, DE)

9:30 Antimicrobial Peptides, Peptidomimetics and Cell-Penetrating Peptides Henrik Franzyk (University of Copenhagen, DK)

10:00 - 10:30 Coffee Break

Session Chairs: Marc Schneider and Mathias Winterhalter

10:30 Invasin Modified Drug Carriers Petra Dersch (HZI, DE)

11:00 Bacteria Movement in Biofilms Romain Briandet (INRA, FR)

Contributed Talks:

11:30 Inhalable Dry Powders for Local Administration of Antimicrobial Peptides Against P. Aeruginosa: Overcoming Lung Barriers Ivana D‘Angelo (University of Naples, IT)

11:40 The Differential Role of Clustering of Glycosaminoglycans for the Uptake of Amphipathic and Arginine-rich Cell-penetrating Peptides Rike Wallbrecher (Radboud University Medical Centre, NL)

11:50 Enhanced Pulmonary Delivery and Antipseudomonal Activity of Quorum Sensing Inhibitors Using Ultra-small Solid Lipid Nanoparticles Noha Nafee (University of Alexandria, EG)

12:00 Selective Delivery of Active Agents to Antigen-presenting Cells by TargoSphere Nanocarriers: Implications for the Treatment of Tuberculosis Ulrich Kalinke (Twincore, DE)

12:10 - 13:40 Lunch Break

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

3

Non Cellular Barriers: Mucus and Surfactant

Session Chairs: Ulrich F. Schäfer and Catherine Nordgård

13:40 Mucus and Surfactant as Barriers to Pulmonary Drug Delivery Claus-Michael Lehr (HIPS, DE)

14:00 Lung Surfactant - A Barrier or a Shuttle for Drug Delivery? Jesus Perez-Gil (University of Madrid, ES)

Contributed Talk:

14:30 Bio-inspired Hybrid Alveolar Surfactant Modified Nanogels Mediate Improved siRNA Delivery Lynn de Backer (Gent University, BE)

14:40 Thinking Alternatively:Transport of Nanoparticles across the Buccal Mucosa Eva Roblegg (University of Graz, AT)

15:10 - 15:40 Coffee Break

Session Chairs: Claus-Michael Lehr and Christian Ruge

15:40 Particle Transport Through Mucus Jung Soo Suk (Johns Hopkins School of Medicine, US)

16:10 Studying the Transport of Nanoparticles in Burkholderia Multivorans Biofilms and Cystic Fibrosis sputum with Use of Single Particle Tracking Microscopy Kevin Braeckmans (Ghent University, BE)

16:40 Mucus in Gastrointestinal Drug Delivery: A Dynamic Barrier of Complexity Catherine Nordgård (University of Trondheim, NO)

Contributed Talks:

17:10 Biosimilar Mucus as an in vitro Model for Assessment of Intestinal Drug Absorption Marie Boeg (University of Copenhagen, DK)

17:20 Nanoparticle Transport Across the Mucus Barrier - Analysis via Microchip Array Aline Moosmann (IBMT, DE)

17:30 Overcoming the Mucus Barrier with Highly Densely Charged Nanoparticles: A New Approach Irene Pereira de Sousa (University of Innsbruck, AT)

17:40 Dendritic Core-Multishell Nanocarriers for the Delivery of Active Compounds Michael Unbehauen (Free University of Berlin, DE)

17:50 From Colloids to Capsules: Novel Pathways to Multifunctional Inorganic Microcapsules Michael Maas (University of Bremen, DE)

18:00 Poster Viewing

19:00 Reception with Food and Drinks at the Science Park

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

4

Tuesday Feb 18, 2014Conference Start: 8:15 a.m.

Overcoming the Skin Barrier

8:15 Welcome Address by Volker Linneweber, President of Saarland University

Session Chairs: Marc Schneider and Michael Roberts

8:30 Hair follicles – An Efficient Target for Drug Delivery Jürgen Lademann (Charité Berlin, DE)

9:00 Laserporation for Vaccination and Specific Immunotherapy Richard Weiss (University of Salzburg, AT)

9:30 Tight Junctions in Healthy and Diseased Skin Johanna Brandner (University Hospital Hamburg, DE)

10:00 Skin Defensins Jürgen Harder (University of Kiel, DE)

10:30 - 11:00 Coffee Break

Modelling of Skin Absorption

Session Chairs: Ulrich F. Schäfer and Richard Mendelsohn

11:00 Extension of the Jmax Concept Michael Roberts (University of Queensland, AU)

11:30 Mathematical Description of Transdermal Delivery form Silicone Matrices Már Másson (University of Iceland, ISL)

11:50 Computational Modelling of the Skin Barrier Dirk Neumann (Scientific Consilience, DE)

Contributed Talk:

12:20 A predictive microscopic mathematical diffusion model for finite dose skin penetration: Extension of the model considering stratum disjunctum layers Michael Heisig (Goethe University Frankfurt, DE)

12:30 - 14:00 Lunch Break

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

5

Skin Barrier - Analytical Techniques

Session Chairs: Claus-Michael Lehr and Jürgen Lademann

14:00 Correlative Microscopy of the Human Skin Barrier Roger Wepf (ETH, CH)

14:30 Infrared Spectroscopy and Microscopic Imaging: Applications to Biophysics and Skin Pharmacology Richard Mendelsohn (Rutgerns University, US)

15:00 Chemically Selective Visualization of Human Skin by Confocal Raman Microscopy Maike Windbergs (Saarland University, DE)

15:30 - 16:00 Coffee Break

Contributed Talks:

Session Chairs: Maike Windbergs and Johanna Brandner

16:00 Image Contrast Enhancement by FLIM for Characterization and Tissue Localization of Nanocarriers Ulrike Alexiev (Free University Berlin, DE)

16:10 Transfollicular Vaccination Ankit Mittal (HIPS, DE)

16:20 Development of Hyaluronic Acid Based IgG-loaded Dissolving Microneedles for Intradermal Protein Delivery Juha Mönkäre (Leiden University, NL)

16:30 Raster Image Correlation Spectroscopy on Liposomes Jonathan Brewer (University of Southern Denmark, DK)

17:00 Transdermal Delivery of Macromolecules Samir Mitragotri (University of California, US)

17:30 Poster Viewing

18:45 Departure to Conference Dinner by Bus

19:00 Conference Dinner at Casino am Staden

10thInternational Conference and Workshop on

BIOLOGICAL BARRIERS 16-21 February 2014

6

Wednesday Feb 19, 2014Conference Start: 8:15 a.m.

In Vitro Models of Biological Barriers 8:15 Welcome Address by Mardas Daneshian, Center for Alternatives to Animal Testing-Europe (CAAT)

Session Chairs: Brigitta Loretz and Eleonore Haltner

8:30 Human Skin Models Joke Bowstra (Leiden University, NL)

9:00 Lung Models in Medicine and Toxicology Donna Davies (University of Southampton, UK)

9:30 Lung Models in Drug Delivery Nicole Daum (HIPS, DE)

10:00 Poster Award

10:10 - 10:40 Coffee Break

Session Chairs: Nicole Daum and Donna Davies

10:40 Dynamic Models of the Gastrointestinal Tract Heike Walles (University of Würzburg, DE)

11:10 Human-on-a-Chip - Combining Organ Equivalents into Systemic Arrangements in vitro Uwe Marx (Technical University of Berlin, DE)

Contributed Talks:

11:40 Development of Three Dimensional Basement Membranes Angela Rossi (University of Würzburg, DE)

11:50 Steps towards the future: Replacement of human studies by validated in vitro barrier models: EU Marketing authorization for an oral prednisolone formulation Eleonore Haltner (Across Barriers, DE)

12:00 Closing Remarks by the Programm Chairs and Organisers

12:15 End of Conference