Bioinformatics support at School of Biological Sciences University of Essex Igor Chernukhin Steven Yates
Jan 21, 2016
Bioinformatics support at School of Biological Sciences
University of Essex
Igor ChernukhinSteven Yates
Bioinformatics support
NGS transcriptome analysisChip-seqMicroarray analysis
Bioinformatics support
NGS transcriptome analysis Fast sequence-aligning tools working in parallel
computing environment Static software for transcriptome analysis and R or Matlab workframe Network analysis & Graphical presentation
Chip-seqMicroarray analysis
Transcriptomics RNA-seqWorkflow (example)
Filtering illumina reads from contaminating bacterial sequences
Transcriptome assembly
Counting reads
NGS transcriptomics
Transcriptome annotationTypically 60 - 70% transcripts should
be annotated with Uniprot
Analysisof Differentially Expressed Genes
Clustering analysisof expression data
GO enrichmentanalysis
DEG analysis in R::edgeR (example)NGS transcriptomics
Dispersions
Gene Ontology - enrichment analysis
NGS transcriptomics
R::topGO:Evaluates overrepresentation of GO
terms in DEG using several statistical models
Revigo: Updating/removing redundant GO terms
Cytoscape: building network of activated biological processes
GO - enrichment network(example)
NGS transcriptomics
Data clustering analysisfor time-series datasets:
finding similar expression profile
NGS transcriptomics
Matlab or R workspace:Choosing clustering algorithm
Analysis of individual clusters
Data clustering analysis (example)
NGS transcriptomics
Finding similar expression profile
Data clustering:analysis of individual clusters (example)
NGS transcriptomics
Response to salt
967, 7728, 12711: Iron starvation induced protein (Ferritin)
NGS transcriptomics
5628, 11741, 21761: Protein heat shock protein Hsp7015404: Eukaryotic translation initiation factor
Response to salt & cold
Data clustering:analysis of individual clusters (example)
NGS transcriptomics
Biological data modelling
Inferring gene networkwith Variational Bayesian State Space
Modelling
VBSSM will generate a network of gene interactions from time series expression data by treating the data as linear dynamical system, also known as a state-space model.
Plant bioinformatics
Online tools
http://bsproteomics/PlantBinfo/
Plant bioinformatics
Room 5.41
Igor Chernukhin (igorc)Steven Yates (sayates)
Bioinformatics support at School of Biological Sciences
University of Essex