Bioinformatics on Proteomics Hsueh-Fen J uan April 24, 2 003 NTNU
Dec 20, 2015
Outline Introduction Primary Structure Analysis Protein Sequence Searching Protein Sequence Alignment Proteomics Tools Protein Identification Useful Bio-websites
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
Introduction ExPaSy: Expert Protein Analysis System SWISS-PROT: Protein knowledgebase TrEMBL: Computer-annotated supplement
to Swiss-Prot
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Useful Bio-websites
Primary Structure Analysis Object:
To compute the characters of proteins.
-Amino acid composition
-Atomic composition
-pI
-Molecular weight
Introduction
Primary Structure Analysis
Protein Sequence Searching
Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
The Information Stored in Genes Is Expressed by a Multistage Process
Eur J Nucl Med 2002, 29, 115-32
Standard Proteome Analysis by 2DE-MS
Current Opinion in Chemical Biology 2000, 4:489–494
Mass Fingerprint Searching in http://www.expasych/tools/peptident.html
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 2500 2600 2700 2800 2900m/z0
100
%
kcw1212-1 14 (1.129) Cn (Cen,4, 50.00, Ht); Sm (SG, 1x2.00); Sb (15,10.00 ); Cm (6:15) 1: TOF LD+ 7.95e31132.917
965.893
966.895
1022.925
1023.925
1791.532
1133.923
1517.239
1134.9241500.215
1189.9631349.2351190.965
1248.008 1350.239
1351.239
1518.245
1519.244
1574.283
1774.5311576.280
1792.532
1793.535
1794.537
2216.8611848.5772168.845
1850.563
1851.577 2126.732
2217.8702218.857
2810.3872344.987
2552.1152732.213 2866.446
Mass spectrometry
Introduction
Primary Structure Analysis
Protein Sequence Searching Protein Sequence Alignment
Proteomics Tools
Protein Identification
Useful Bio-websites
Useful Bio Websites PROSITE
http://tw.expasy.org/prosite/ Amos' WWW links page
http://tw.expasy.org/alinks.html Phosphoprotein Database (PPDB)
http://www-lmmb.ncifcrf.gov/phosphoDB/