Biofilter in-situ incubation Ken Collins Southampton Oceanography Centre
Dec 31, 2015
Biofilter in-situ incubation
Ken CollinsSouthampton Oceanography Centre
Sampling pump
mixing pump
Oxygen electrode
Control – seawater only
Biofilter + seawater
Pump batteries
Winkler bottle
Oxygen meter
Biofilter incubation 25.4.02
time mins
0 20 40 60 80 100 120 140 160 180 200
Oxy
gen
conc
entr
atio
n m
ol l-1
100
120
140
160
180
200
220
240
260
280
300
chamber Winkler chamber regressioncontrol Winklercontrol regression
O2 = 276.4 - 0.728 * mins
r2 = 0.97
faunal community respiration rates
Temperature oC
4 6 8 10 12 14 16 18 20 22
oxy
gen
con
sum
ptio
n
mo
lO2 g
AF
DW
-1 h
-1
0
10
20
30
40
50
60
s
s
s
o
o
o
r
rrr r
r r
r
s - Southamptono - Obanr - artificial reef
Slovenia +
Soton & Oban biofilter incubations
group
ascidians barnacles crustacea hydroids molluscs worms
prop
ort
ion
AF
DW
0.0
0.2
0.4
0.6
0.8
1.0
28.3.0225.4.0216.9.0217.9.0218.9.02
Temperature effect Q10
ln Q10 = [10/(T2-T1)] ln k2/k1
if Q10 = 2,, T2 = 20oC, T1= 15oC
ln 2 = [10/(20-15)] ln k20/k15
.693 = 2 ln k20/k15
k20= 1.414 k15
faunal community respiration ratesnormalised to 20oC using Q10 = 2.0
incubation temperature oC
8 10 12 14 16 18 20 22
oxy
gen
co
nsu
mp
tion
m
olO
2 gA
FD
W-1
h-1
20
30
40
50
60
70
80
90
100
s
s
o
o
o
r
r
rr
r
rr
r
s - Southamptono - Obanr - artificial reef
Slovenia +
Average respiration rate at 20oC
= 53 +/_ 16 umolO2 gAFDW-1 h-1
oxygen consumption- carbon flux (1mole O2 = 1mole C)- nutrient flux (Redfield ratio
C:N:P=106:16:1)- filtration rate