Biocontrol Justin Kim. Need Knowledge Base Jorge M. Vivanco (2002) Harsh Pal Bais (2000)

Biocontrol

Justin Kim

Need

Knowledge Base

Jorge M. Vivanco (2002)Harsh Pal Bais (2000)

R. Charudattan (2006)R. Charudattan (2006)

Biocontrol

Biocontrol Agent

• (Elizabeth and Emmert, 1999).

Bacillus Subtilis

Kung-Ta Lee (1993) Kung-Ta Lee (2001)

Literature Review

Fall, Ray (2004)

Disease

Ross Ballard (2008)

Jason Brantner (2004)

Arabidopsis/SoybeanN= 120

Control Group-Seeds grown in normal environmentN=30

V3- Plant inoculated with Pseudomonas Putida from fusariumN=30

V2- Plant inoculated with bacillus subtilis from the fusariumN=30

Mortality of the plants are comparedMean +- SDTwo way Anova

V1- plant grown with the fusariumN=30

Methodology Flow Chart

Do-Ability

• Biocontrols available from Online Carolina Catalog

Purpose-

To find biocontrol agents that works better against certain diseases

Hypothesis-Alternative- Bacillus subtilis worked better against the disease

Alternative- Pseudomonas putida worked better against the disease

Budget PlanVendor Item # Quantity Description Price Total

Carolina Online 154921 1 Bacillus subtilis, Living, Tube $9.20 $9.20

Carolina Online 156033 1 Fusarium Oxysporum $9.20 $9.20

Carolina Online 177600 1 Arabidopsis: Columbia (Col-0) Seed, Pk 200 $10.50 $10.50

Carolina Online 593120 1 Walter QZG Zoom Stereomicroscope $519.00 $519.00

BD CATALOG 442104 1 Diluting fluid- used to count Bacteria

Carolina Online 741270 1 Petri Dish, Polymethylpentene, 100 x 15 mm, each $6.45 $6.45

Carolina Online 195700 1 Murashige and Skoog Salt Base, Powdered, 10 x 1 L $16.05 $16.05

Carolina Online 155265 1 Pseudomonas putida, Living, Tube $9.20 $9.20

Bibliography

• Baker et al., "Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves" Phytopathology 73:1148-1152 (1983).

• Cavaglieri L., Orlando J., Rodríguez M.I. - Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level- Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km, 601, (5800) Río Cuarto, Córdoba, Argentina

• Elizabeth AB, Emmert HJ. Biocontrol of plant disease: A (Gram-)positive perspective. FEMS Microbiol Lett. 1999;171:1–9

• Estruch et al., "Transgenic plants: An emerging approach to pest control" Nature Biotechnology 15:137-141 (1997).• G. Heidi . Elnaga, Abo - Bacillus subtilis as a Biocontrol Agent for Controlling Sugar Beet Damping-off Disease -Egypt.

J. Phytopathol., Vol. 34, No.1, pp. 51-59 (2006)• Olsson J, Bőrjesson T, Lundstedt T, Schnürer J. Detection and quantification of ochratoxin A and deoxynivalenol in

barley grains by GC-MS and electronic nose. Int J Food Microbiol. 2002;72:203–214.• Onji Y, Aoki Y, Tani N, Umebayashi K, Kitada Y, Dohi Y. Direct analysis of several Fusarium mycotoxins in cereals by

capillary gas chromatography-mass spectrometry. J Chromatogr A. 1998;815:59–65.• Placinta CM, D’Mello JPF, Macdonald AMC. A view of worldwide contamination of cereal grains and animal feed

with Fusarium mycotoxins. Anim Feed Sci Tech. 1999;78:21–37.• Pelczar, Michael. Pelczar, Rita- “plant disease”- Encyclopædia Britannica. 2009. Encyclopædia Britannica Online• Singh et al., "Bacillus subtilis as a control agent against fungal pathogens of citrus fruit" Trans. Br. Mycol. Soc.

83(3):487-490 (1984).• Williams & Wilkins- Bergey's Manual of Sytematic Bacteriology, , vol. 2, pp. 1123-1124, 1126, 1133 (1986).• Xin Zhang,1 Bing-xin Zhang,†‡1 Zhen Zhang,2 Wei-feng Shen,2 Ching-hong Yang,3 Jing-quan Yu,4 and Yu-hua Zhao-

Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field- Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China

• Yamada et al., "Biological activity of antifungal substances produced by Bacillus subtilis" J. Pesticide Sci. 15(1):95-96 (1990).