Biocidal Materials for Public Health and Consumer Products (Overview) Available Through TETRAD Technologies Group, Inc. Contact:: Dr. Martin Dudziak, PhD.

Biocidal Materials for Public Health and Consumer Products

(Overview) Available Through

TETRAD Technologies Group, Inc.

Contact:: Dr. Martin Dudziak, [email protected]

(804) 740-0342(757) 847-5511

(202) 415-7295 cell

An Anti-Microbial Monomer/Polymer Offering Cleaner, Faster, Safer, More Comprehensive

Anti-Microbial Protection

Not a serious toxin and danger like Triclosan Cheaper, faster-acting than silver/copper-based compounds Non-leaching; stays within the material Extends functional life of product treated Produces self sanitizing materials Can be applied as spray, in solution, or as powder

Benefits

• Non- leaching, permanent antimicrobial surface• Biocide has been proven to be nontoxic, to contain no carcinogens,

teratogens, mutagens or reproductive toxins• Active kills instantly by disrupting the cell membrane, eliminating

any possibility of mutation, adaptation or resistance• Broad kill range• Environmentally friendly-EPA and FDA approved• The technology can be used on wide range of materials.• Provides both collective and individual bio-defense protection.• Added cost is modest compared to benefit. Approximately 2-5 cents

per square foot of coating or 5 to 50 cents per pound of plastic or resin.

Typical Applications

• Individual and collective protection– Clothing, Uniforms, and other Wearable Fabrics– Carbon filters– Tent Webbing and canvas materials– Painted Surfaces– Laminate and counter tops in field operations

• Contamination avoidance– Bulk polymer Resins/ Plastics– Resin Concentrates– Sanitation and Health Safety Equipment

• Medical treatment– Medical Devices– Surgical drapes and wound dressings

• Nano coatings– Interior surfaces/ walls floors– HVAC ducts– Filters

Action: The Poly-Quat AntimicrobialIs bound to the product controlling Microbes

on contact

Is substantive to the product substrate Is not consumed when challenged by microbes Mechanically destroys the cell Will not cause microbial adaptation Functions equally in a nano coating or bulk substrate treatment

process Can be added into manufacturing process or applied later Can be combined with other surface protectants (e.g., water

repellant, anti-static)

The Antimicrobial AgentWhy did we choose this antimicrobial?

• 3 (trimethoxysilyl)propyl–dimethyloctadecyl ammonium chloride

• Unique in the scope of its kill range/broad killBacteria (gram positive)

Staphylococcus AureusBacteria (gram negative)

Salmonella TyphosaYeastAlgaeFungi

EPA Registered Chemically active

Antimicrobial Treatment Improves Fiber Strength

Mean Yield Strength by Boil Treatment

n = 30

0

2000

4000

6000

8000

10000

12000

14000

16000

18000

20000

Boil No Boil

Boil / No Boil

Mean

Yie

ld S

en

gth

No Base

Sodium Hydroxide

Ammonium Hydroxide

p = 0.002

p < 0.001 p < 0.001 p = 0.045

p < 0.001

ASTM standard D2256-02 : Tensile Strength

Antimicrobial JSLIST Fabric

Sample ID Log Kill

(1) JSLIST w/ 0.2% HM-4072 6.48

(2) JSLIST W/ 0.20% HM-4072 Triton X-100 6.47

(3) 65/35 Polyester/cotton W/ 0.2% HM-4072 and 3.6% Zonyl 7040

>7.41

(3) Boiled in water 5 min 7.22

(3) Laundered 6 times 6.32

(4) 100 % Cotton w/ 0.2% HM-4072 and 3.6 % Zonyl 7040

7.12

(4) Boiled in water 5 min 7.07

Dynamic Shake Flask Test: E. coli, one hour, sample size 2.5 X 2.5 cm

0

1

2

3

4

5

6

7

0 2 4 6 8

Rounds of exposure

Lo

g #

su

rviv

ors

Blank

Test

Repeated Exposure of Treated fabric to E. coli

Dynamic Shake Flask Test-E. coli2.5 cm Sample One hour contact time

Tent Webbing Soil Burial TestPoly Quat

Time Control 1% Poly-QUAT0 100 100

Week 1 118 105

Week 2 112 105

Week 3 80 99

Week 4 63 120

Week 5 35 99

Week 6 30 108

Week 7 Dis 86

Week 8 Dis 85

Week 9 Dis 76

Percent Strength Remaining After Soil Burial

ASTM 30-1999

Antimicrobial Activated Carbon

% HM 4072 % Poly-Quat Log Kill % CTC

0.1 None 77.6

0.25 6.11 73.4

0.5 6.26 75.4

0.05 5.92 75.4

0.01 5.75 77.0

0.25 6.18 78.0

0 (Control) 0 (control) 1.11 E6 (cfu/mL)* 79.5

Dynamic Shake Flask Test: E. coli, one hour, sample size 2.5 X 2.5 cm

* Colony forming units in starting broth. The control exhibits no kill.

Polymer Applications

Material Log Kill

0.2 % active

PVC 6.69

HDPE 6.35

Melamine/formaldehyde 7.1

Polyurethane 6.44

PVOH 5.54

Dynamic Shake flask test, E. coli

Dynamic Shake Flask Test-E. coli2.5 cm Sample One hour contact time

Anthrax Surrogate: Bacillus Cereus

Identification Form Log Kill

Textile/PVOH Cells No Kill

Textile/PVOH/L-alanine Cells No Kill

Textile/PVOH/L-alanine Cells No Kill

Textile/PVOH/0.2% HM4072/L-alanine

Cells 5.79

PVOH cast film/0.2% HM4072/L-alanine

Cells 5.77

PVOH cast film/L-alanine Spores No Kill

PVOH cast film/0.2% HM-4072/L-alanine

Spores 4.16

Dynamic Shake Flask Test-E. coli (1.06E+06)2.5 cm Sample One hour contact time

Resin Coating Systems

Resin System % Poly Quat Log Kill

Water Acrylic Melamine/ Al 0.1 4.73

0.25 5.30

Water Borne Acrylic Urethane/ Al

0.2 3.6

0.4 4.34

0.4 aged 1 week 5.55

Dynamic Shake Flask Test-E. coli2.5 cm Sample One hour contact time

HM 4072 Sanitizing Nano Coating Surface Log Kill

Aluminum 0.2 % from IPA >7.08

Aluminum 0.2 % from Water 7.08

Aluminum 0.2% From Water Leached 1 Hr 4.55

Aluminum 0.02 % from IPA 7.08

Stainless Steel 0.2 % From Water 5.90

Glass Slides 0.2 % from Water 6.24

Laminate 0.2 % from Water 6.31

Dynamic Shake Flask Test-E. coli2.5 cm Sample One hour contact time

Substrate surface

SiHO

OH

OH

N+

CH3

CH3

CH3

Cl-

Octadecylaminodimethyltrihydroxy-silylpropyl Ammonium Chloride

Untreated Control surface

Treated surface

Living Bacteria Dead Bacteria