BIO PacRim conference Ogden.pdfVitamin solution (100 X) Vitamin solution 0.005 0.005 ml Common name g/L Vendor Boric Acid 0.00286 VWR Manganese Chloride 0.00181 VWR Zinc Sulfate 0.000222

BIO PacRim conferenceContact: Kimberly Ogden

Department of Chemical and Environmental EngineeringUniversity of ArizonaTucson, Arizona USA

[email protected]

Project Goals

• Obtain long term algal cultivation data in outdoor pond systems

• Work with industrial, government, and academic partners to advance the algal biofuels, feed, and bio-products industry

• Optimize biomass and lipid content for production of biofuel using impaired waters

• Develop real time sensors and control strategies for efficient cultivation

• Improve and refine cultivation models, as well as system techno-economic models and life cycle assessments.

Testbed Facilities

ARID Raceway US Patent # 8,245,440

Ability to Cultivate GMO Algae in Climate Controlled Green

Houses

Testbed Facilities

Hot house with raceway for seasonal extension

Raceways and Solix System - Centrifuge harvester

Testbed Facilities

Multi-scale Testbed Facility with Harvesting Infrastructure

Cultivation of Chlorella sorokinana

2014

Media Optimization

Required volume (L) = 1

Chemical Common name g/L Total Grams Total Kilograms

Na2CO3 Soda Ash 0.02 0.02 0.00002

NaCl TruSoft 5 5 0.005

(NH2)2CO Urea 0.05 0.05 0.00005

MgSO4 * 7H2O Magnesium sulphate 0.012 0.012 0.000012

NH4H2PO4 MAP 0.01 0.01 0.00001

KCl Potash 0.129 0.129 0.000129

FeCl Iron Chloride 0.0035 0.0035

Di Sodium EDTA 0.00436 0.00436

Trace metals solution 1 1 0.001

Vitamin solution (100 X) Vitamin solution 0.005 0.005 ml

Common name g/L Vendor

Boric Acid 0.00286 VWR

Manganese Chloride 0.00181 VWR

Zinc Sulfate 0.000222 VWR

Copper Sulfate 0.000079 VWR

Cobalt Nitrate 0.0000494 VWR

Sodium Molybdate 0.000391 VWR

Common name g/L Vendor

Vitamin B12 0.135 VWR

Manganese Chloride 0.025 VWR

Zinc Sulfate 0.335 VWR

Trace metals solution

Vitamin solution

Pe-06 (or Pe-02_B)

Semi continuous Operation

0

0.5

1

1.5

2

2.5

3

3.51

0/1

5/1

4

10

/17

/14

10

/19

/14

10

/21

/14

10

/23

/14

10

/25

/14

10

/27

/14

10

/29

/14

10

/31

/14

11

/2/1

4

11

/4/1

4

11

/6/1

4

11

/8/1

4

11

/10

/14

11

/12

/14

11

/14

/14

11

/16

/14

11

/18

/14

11

/20

/14

11

/22

/14

11

/24

/14

OD

75

0

Continuous pH, Temp, DO

-10

-5

0

5

10

15

20

25

30

35

40

45

0

1

2

3

4

5

6

7

8

9

10

10/18/2014 10/23/2014 10/28/2014 11/2/2014 11/7/2014 11/12/2014 11/17/2014 11/22/2014 11/27/2014

pH

OD 750

DO

Temp C

CO2 input

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

10.00

10/24/2014 10/26/2014 10/28/2014 10/30/2014 11/1/2014 11/3/2014 11/5/2014

Cu

mm

ula

tive

CO

2 (

L)

pH

an

d O

D 7

50

OD 750

pH

CO2

Culture Diagnostics

• PCR diagnostics of invasive strains identified by 18S rRNA gene fragment sequencing

• Specific primers for algae and bacterial contaminants

• Flow Cam Systems for monitoring

• Real time OD monitor and flow cell –Invention disclosure and patent

• Contaminant Control to maintain Chlorella sp.

Molecular Based Culture Diagnostics

Testbed Facilities

Climate Controlled Cultivation Systems for Growth and Biomass

Optimization and Modeling

Characterizing Cold Weather Strains

Opportunities for Collaboration

• Production of algal biomass for a variety of applications – Fuel– Feed– High value Products

• Cultivation of genetically modified algae

• Design and implementation of integrated, controlled systems for cultivation, harvesting, and conversion.

• Optimization of algal productivity in impaired waters

• Culture diagnostics using molecular markers