Bio 230 - Microbiology - Spring 2013 Study Guide 13 http://www.answersingenesis.org/docs2004/images/xylitol_comic.gif
Bio 230 - Microbiology - Spring 2013
Study Guide 13
http://www.answersingenesis.org/docs2004/images/xylitol_comic.gif
How DNA Sequencing Works
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
Part of a radioactively labelled sequencing gel
http://en.wikipedia.org/wiki/DNA_sequencing
Reaction with all four of the dideoxy nucleotides (A, G, C and T)
present, and with different fluorescent colors on each.
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
One reaction can get the sequence of a fragment of DNA as long
as 700 or so nucleotides
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
DNA sequence (455 nucleotides of the lysU gene of E. coli) which
was generated by an automated sequencing device.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/DNA_sequence.gif
Overview of the 454 sequencing technology
The development and impact of 454
sequencing Jonathan M Rothberg & John H Leamon
Nature Biotechnology 26, 1117 - 1124 (2008)
Published online: 9 October 2008
doi:10.1038/nbt1485
454 Sequencing
454 Sequencing
Shotgun Sequencing: Assembly of Random Sequence Fragments
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
http://cancergenome.nih.gov/media/whitehead_center_image.jpg
Large-scale Sequencing Center
http://wishart.biology.ualberta.ca/basys/cgi/submit.pl
Automated, in-depth annotation of bacterial genomic
(chromosomal and plasmid) sequences
Expanded view of the E. coli K-12 genome showing gene location
and COG functional categories
Genome Resources for Bio 230
http://www.geneseo.edu/~simon/bio230/genome_resources.html
Two-colour microarrays, the probes are
oligonucleotides, cDNA or small
fragments of PCR products
corresponding to mRNAs. This type of
array is typically hybridized with cDNA
from two samples to be compared (e.g.
patient and control) that are labeled
with two different fluorophores (e.g.
Rhodamine (red) and Fluorescein
(green)).
http://en.wikipedia.org/wiki/DNA_microarray
http://en.wikipedia.org/wiki/DNA_microarray
PCR
RNA Transcription Levels Can be Validated with qPCR
Figure 5. Distribution of cell cycle-regulated genes within functional
categories. The number of genes in each category is in parentheses.
Genes were assigned to a functional category based on the classification
scheme defined by Clusters of Orthologous Genes (COGs)
The End