Bio -1000F Test Reference Exposure Time x2 Exposure Time x2 Exposure Time x4 Exposure Time x4 Following electrophoresis (voltage: 50 V), the images were caught by Bio-1000F with different exposure times. 5 μl of Bio100 TM Mass DNA Ladder (Protech Technology Enterprise Co., Ltd.) was loaded into line 1 (2 0 ). Twofold serial dilutions of the ladder were prepared in 1x DNA loading dye and loaded into lanes 2 to11 (2 -1 to 2 -10 ). 5 μl of Bio100 TM Mass DNA Ladder (Protech Technology Enterprise Co., Ltd.) was loaded into line 1 (2 0 ) of 2% TAE agarose gel. Twofold serial dilutions of the ladder were prepared in 1x DNA loading dye and loaded into lanes 2 to11 (2 -1 to 2 -10 ). The images were caught by Bio-1000F with different exposure times. Following electrophoresis (voltage: 50V), the gel were immersed into 1x TAE buffer with 1/20,000 Diamond™ Nucleic Acid Dye for 30 minutes at 30°C . Protocol Result Manufacturer : Promega Stain : Diamond™ Nucleic Acid Dye DNA Electrophoreis Post-Staining Precast Gel 3 μl Diamond™ Nucleic Acid Dye in 60 ml 2% TAE agarose gel. 2 3 1 1 3 2
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Bio -1000F Test Reference
Exposure
Time
x2Exposure
Time
x2
Exposure
Time
x4Exposure
Time
x4
Following electrophoresis (voltage: 50 V), the images were caught by Bio-1000F with different exposure times.
5 µl of Bio100TM Mass DNA Ladder (Protech Technology Enterprise Co., Ltd.) was loaded into line 1 (20). Twofold serial dilutions of the ladder were prepared in 1x DNA loading dye and loaded into lanes 2 to11 (2-1 to 2-10).
5 µl of Bio100TM Mass DNA Ladder (Protech Technology Enterprise Co., Ltd.) was loaded into line 1 (20) of 2% TAE agarose gel. Twofold serial dilutions of the ladder were prepared in 1x DNA loading dye and loaded into lanes 2 to11 (2-1 to 2-10).
The images were caught by Bio-1000F with different exposure times.
Following electrophoresis (voltage: 50V), the gel were immersed into 1x TAE buffer with 1/20,000 Diamond™ Nucleic Acid Dye for 30 minutes at 30°C .
Protocol
Result
Manufacturer : PromegaStain : Diamond™ Nucleic Acid Dye
DNA ElectrophoreisPost-StainingPrecast Gel
3 µl Diamond™ Nucleic Acid Dyein 60 ml 2% TAE agarose gel.
2
3
1 1
3
2
Dilution
Times
104
Dilution
Times
105
Dilution
Times
106
Result
Decreasing the concentration of Diamond™ Nucleic Acid Dye in precast gels can solve the problem of
smeared and discrepant DNA migration.
Bio -1000F Test Reference
Improvements for Nucleic Acid Gel Stain
Dilution
Times
5x106
( Exposure time: x4 ) ( Exposure time: x4 )
( Exposure time: x12 ) ( Exposure time: x12 )
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