Beyong probiotic and prebiotic a synbiotic health supplement

Vitafoods 2013, May 14th F. Pousset Copyright

BEYOND PROBIOTIC AND PREBIOTIC

A SYNBIOTIC HEALTH SUPPLEMENT

SHS

FOR INTESTINAL HEALTH AND QUALITY OF LIFE

Florence Pousset


  Synbiotic: literature survey

Probiotics Prebiotics Synbiotics

Num

ber o

f pub

licat

ions

Years

1400

1200

1000

800

600

400

0

200


  Synbiotic: definition

If a synbiotic relationship is intended, then it should be verified scientifically

SYMBIOSIS

‘A mixture of probiotics and prebiotics that beneficially affect the host by improving the survival and the implantation of live bacteria in the gastrointestinal tract of the host’

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  Synbiotics efficacy : experimental studies

Rowland et al. 1998

azoxymethane A

bera

nt c

rypt

foci

/ co

lon

Control Inulin B. longum SynB β-gl

ucos

idas

e ac

tivity

Colorectal cancer

Synbiotics efficacy probiotics or prebiotics efficacy only in decreasing abnormal crypt foci and increasing β-glucosidase activity

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Control Inulin B. longum SynB


  Synbiotics efficacy : clinical studies

Fujimori et al., 2009 Treatment Total IBDQ score (Week 0 – Week 4)

Cross-over RCT B. longum (2e9) 162 - 169

Patients with ulcerative colitis (n = 120) Psyllium (8g/dose) 174 - 182

Synbiotic 168 – 176 (p<0.03)

Worthley et al., 2009 Treatment Abdominal bloating

Bowel movements

Gurgling

Cross-over RCT B. Lactis (e9) 78.9% 63.2% 89.5%

Healthy volunteers (n = 20)

High amylose maize starch (25g/dose)

77.8% 66.7% 83.3%

Synbiotic 61,1% 88.9% 94.4%

IBDQ: Inflammatory Bowel Disease Questionnaire

Synbiotic therapy could prove more effective than therapies based on prebiotics or probiotics


 Non severe digestive troubles

More than half of the population report at least one of these symptoms

External influences

•  Diet •  Stress •  Drugs i.e. antibiotics •  Travel •  Illness •  Psychological factors

Symptoms

•  Abdominal pain 63% •  Diarrhoea 55% •  Bloating 53% •  Flatulence 44% •  Constipation 44% •  Other 15%


Synbiotics

  Gallup for non severe clinical digestive troubles

Core Foundation study 2010



SHS synbiotic

Synbiotic: double effect

Prebiotic « Novel Fiber » Low calorie content 3.3 Kcal/g Low glycaemic index < 28 Low sodium content

4% B. longum 109 cfu/g

Corn Soluble fibers Carbohydrates

K+ Mg++ B6

 Synbiotic Health Supplement

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 Preclinical study results: in vitro SHS synbiotic

Bifidobacterium longum is sensitive to all tested prebiotics SHS prebiotic novel fiber

Short chain fructooligosaccharides Inulin

Acacia gum Galactooligossacharides

SHS prebiotic induces the highest and earlier B. longum growth than commercially available competitors


 Preclinical study results: in vivo SHS synbiotic

SHS synbiotic induces a 13% increase of rat forelimb grip strength

Nutriose, SHS prebiotic novel fiber and B. longum administered independently have no effect

Muscle strength evaluation: Grip test


  Rat digestive parameters

P 1 4 16 64 SHS (g/day)

Jejunum crypt height

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Intestinal cellular permeability

p < 0.0011 Week 6

P 1 4 16 64 SHS (g/day)

Cry

pt v

illi h

eigh

t (µ

m)

(FD

4) 1

0-4 m

g/m

l pla

sma

Jejunum trophicity might favor nutrient absorption

Decreased cellular permeability might increase resistance to pathogens

SHS synbiotic


  Rat colonic transit time SHS

Cumulative faecal pellet output

Week 3 P

1 g/day

4 g/day

64 g/day

16 g/day

Num

ber o

f fae

ces

Time (min.) 15 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240

SHS synbiotic regulates colonic transit time according to the dose


Change of colon circular muscle contractility

 Jejunum trophicity

Unchanged blood biological parameters

  Total fecal anaerobes Facultative anaerobes

  Functional digestive properties

SHS

  Intestinal permeability

Transit time regulation

Forelimb grip strength improvement

SHS synbiotic


IN NON SEVERE DIGESTIVE TROUBLES IN HUMAN


  Observational study 1

SHS (n = 30)

7 14 28 42 56 Days

Qg

QoL

0

Healthy volunteers 18 – 75 years

n = 60

- 14

Placebo (n = 30)

Escalating dose : 2, 4, 6, 8 g/day

Quality of life questionnaire (QoL): 11-item quality of life, to evaluate: physical sensations and psychological symptoms related to their digestive troubles. Score from 1 to 10, from "no symptom" to "extremely severe symptoms" intensity

General questionnaire (Qg): 16-item general questionnaire to evaluate non severe clinical digestive troubles (transit time and regularity, bloating, gas, stool consistency and frequency…)

SHS synbiotic


Stool consistency Sc

ore

(%)

Day 7 Day 42

Placebo

Day 7 Day 42

SHS

  Efficacy in gut function

SHS synbiotic increase moulded stool consistency And did not induce diarrhoea as common laxatives

Significant decrease of transit activity and regularity Significant decrease of stool number

SHS synbiotic


SHS synbiotic

  SHS synbiotic significant decrease Bloating Digestive comfort Heaviness Tends to decrease the frequence of flatulences

  Efficacy in non severe clinical digestive troubles


SHS synbiotic

SHS synbiotic induces a significant better general-well being a significant feeling of being more relaxed

  Quality of life: mood changes

Transit improvement

Irritability Annoyed Stress Frightened

Scor

e (%

)

Day 7 Day 42

Day 7 Day 42

Placebo

SHS


  Observational study 2 SHS

SHS (n = 24)

14 28 42 56 Days

Dyn

Qg

0

Healthy volunteers 65 – 75 years

n = 48

- 14

Placebo (n = 24)

Standard dose: 4 g/day

Dynamometer (Dyn): evaluation of biceps muscle strength

General questionnaire (Qg): 15-item grouped into three dimensions, general well-being, muscle strength / vitality and non severe digestive troubles (transit time, bloating, gas…)


Day 0 – Day 56

  Synbiotic and muscular strength SHS

Day 0 - Day 28

SHS Placebo

- 6 - 4 - 2 0 2 4 6 Muscle strength (kg)

- 6 - 4 - 2 0 2 4 6 Muscle strength (kg)

SHS synbiotic: a long lasting energy supplier

< 0: 17% 0 < 46% < 2 > 2: 37%

< 0: 45% 0 < 46% < 2 > 2: 9%

< 0: 41% 0 < 50% < 2 > 2: 9%

< 0: 17% 0 < 33% < 2 > 2: 50%


  SHS functions

Beneficial bacteria

Low GI carbohydrates B6, K+, Mg++

Energy

Biomass

Faeces

Non severe digestive Troubles improvement

Metabolic activity

Mood changes

Quality of life improvement

Bowel

Systemic

Psychologic

Muscle strength

Energy / Vitality

Transit regulation

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SHS synbiotic


  Synbiotics efficacy prebiotics or probiotics used independently

  SHS synbiotic activities Transit regulation Non severe digestive troubles improvement Well-being

  Potential applications Sportmen Elders Convalescents Diabetics

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 Take home messages SHS synbiotic


Thank you

SHS

G.E.RM. INNOPHARM JOINT PHARMA RESEARCH ABC CONSEILS

Beyong probiotic and prebiotic a synbiotic health supplement

Health & Medicine

synbiotic relationship

prebiotics efficacy

synbiotics efficacyprobiotics

longum 2e9patients

longum synb rowland

abnormal crypt foci

intestinal health

mixture of probiotics