BBI Solutions - Human Proteins

Human Proteins

“With over 200 different proteins

available, we cater for all of your diagnostic

needs”

We offer both native and recombinant human proteins to ensure supply sustainability for our customers

Bulk capabilities – Available is sizes from 100ug to kg quantities

Quality systems - Operating to ISO 13485 we guarantee product quality, traceability and comprehensive documentation

Custom analysis - with global labs and hospitals, we can access many diverse testing platforms, providing you with the exact analysis results you need

Responsibly managed supply - over 25 years experience sourcing human biologicals at our HTA approved site

Bulk human protein production

Our human proteinsAcute Phase/Inflammation Markers Haptoglobin α-1-Acid Glycoprotein

C-Reactive Protein Ferritin- Liver Tumour Necrosis Factor Alpha

Hormones & Cytokines

FSH Growth Hormone hCG β-hCG Parathyroid Hormone (PTH) PAPP-A Prolactin SHBG

Renal Markers Albumin Cystatin C Lipocalin (NGAL)

RBP

α 1-Microglobulin (α1M) Tamm Horsfall

Glycoprotein Prealbumin

Immunoglobins IgA IgG IgM IgF Kappa Light Chain Lambda Light Chain

Tumour Markers Alpha Fetoprotein (AFP) Beta 2-Microglobulin

(β2M) CA125 CA15-3 CA19-9 CYFRA-21 PSA

PSA-ACT Complex PSA non Binding PAP Neuron specific Enolase Thyroid Peroxidase

Complement & clotting proteins C3c C4c Complement C1q D-Dimer Plasminogen

Cardiac Markers Apolipoprotein A1 (Apo

A1)

Apolipoprotein E (Apo E) Apolipoprotein B (Apo B) Beta-2 Glycoprotein 1 Creatine Kinase MB(CK-

MB) Creatine Kinase MM(CK-

MM)

High-Density Lipoprotein

Low-Density Lipoprotein

Myeloperoxidase (MPO)

Myoglobin

NT-Pro BNP

Troponin Complex

Troponin I

Troponin T

Infectious Diseases HIV TB

Malaria Clostridium difficile Toxin A PBP2a recombinant

protein

Plasma proteins Albumin

1 antitrypsin Butyryl Cholinesterase Alpha-2 macroglobulin Transferrin Transferrin Receptor HbA1c Ceruloplasmin

Autoimmune Markers Thyroxine Binding

Globulin Thyroglobulin Alpha 1 anti

chymotrypsin

>96% purity, minimising the risk of interferences due to contaminants

<3% dimer content ensuring the NGAL protein is highly specific for acute kidney injury

A faster response time, compared to other standard biomarkers

High quality, manufactured in the UK, under ISO 9001 Quality Management System

NGAL is a novel marker for the early diagnosis of acute kidney injury, our new product is available for sampling now and offers:

NEW – Recombinant Human NGAL

Highly specific to kidney injury

Results demonstrate that recombinant NGAL meets the required purity specification and is low in dimer

1. Novex Sharp Pre-stained Marker

2. Recombinant NGAL (non reduced sample)

1 2

New in 2015

Recombinant Galectin-3

Recombinant Procalcitonin

Recombinant Troponin I

and T

Recombinant from E.coli ≥ 96% pure Product available for

sampling

Troponins I and T levels become elevated in the blood within 3 or 4 hours after heart attack and may remain elevated for 10 to 14 days.

A novel Biomarker for chronic heart failure.

Increasingly used as a diagnostic marker for

various cancers.

From human cell line ≥ 95% pure Lyophilised Prototype samples

now available for evaluation

A promising marker for the early diagnosis of

clinically relevant bacterial infections and

sepsis.

≥ 96% pure Lyophilized From Escherichia Coli Prototype samples now

available for evaluation

Custom development and manufacture

Feasibility Optimisation Verification

Recombinant Screening of gene

databases E.coli & Pichia Cloning Transformation Small scale

fermentation

Native Sourcing of raw

materials of human origin

Small scale extraction and purification

Recombinant Optimised growth

conditions Formulation Downstream

processing Purification Fermentation capacity

of 30L per batch

Native Large scale extraction Downstream

processing Purification

Native & Recombinant HPLC Western blot Protein

electrophoresis IEF Gels Commercial

autoanalysers Stability trials Validation

Success story

Gene expression and purification of recombinant Lysin

The Challenge

A customer required two forms of recombinant Lysin however could not find these commercially and did not have the capabilities to manufacture them

The Solution

Our R&D team delivered the 2 variants to specification, timescale and budget

DNA Synthesis

Expression in bacteria

DNA Design Cloning Transformation Purification Characterisation

DNA gel