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Trng i hc Tr Vinh Bo co thc hnh ha phn tch 2 Khoa Ha Hc ng Dng

Trng i hc Tr Vinh Bo co thc hnh ha phn tch 2 Khoa Ha Hc ng Dng

TRNG I HC TR VINHKHOA HA HC NG DNG----------

BO CO THC HNHHA PHN TCH 2 Ngi hng dn: Hunh Th Hng Hoa Sinh vin thc hin: Nhm 2 L Th Thanh Hng Nguyn Trng Hu Nguyn Vn Khang Nguyn Tho LinhTr Vinh, 27 /8/2014

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Tr Vinh, ngy thng nm 2014 Ngi hng dn

Hunh Th Hng Hoa

VN HNH MY GCHNG DN TOTALCHROM CHO PHIN BN 6.3

I. Gii thiu1. Quyn s dng? ng k phn mm s dng Chng ta phi ng k vi PerkinElmer phn mm h tr bn c th s dng chng trnh h tr v c thng bo v ci tin phn mm cng nh bt k vn no khc. Bao gm nhng gi sau: TotalChrom Client/Server: Tho thun cp php phn mm ng k ca bn p ng yu cu ny. TotalChrom Workstation: Chng ta phi in vo giy php vnh vin ca bn PAK ng dng v gi cho PerkinElmer bng cch lm theo cc ch dn trn hnh thc. TotalChrom Turnkey : bao gm 2 gi trn.Lu : Ngi dng Turnkey ch nn thc hin theo cc hng dn ci t th cung cp vi h thng ca h v b qua tt c cc phn trong hng dn ny c lin quan ci t. Ngi dng v my tnhVic u tin bn phi thnh tho vi my tnh ca bn. lm quen vi cch c ti liu trn Microsoft Window. Tm hiu chng, nu bn vn khng hiu c th tm mua ti liu cc ca hng a phng cng nh trng hc, s cng ngh,

Ngi dng mi v Microsoft WindowsNhng bc bn nn thc hin khi lm quen vi phn mm Total Chrom:1. Cc my tnh ca bn phi c ci t t Perkin Elmer m bo mc tng thch ca phn mm.2. ng c gng vn hnh khi bn cha hiu v n.3. Phi chc rng h iu hnh ca bn lun hot ng bnh thng, ng tc phn mm c th chy n nh.2. Gii thiu v ci t Lu : H thng Turnkey c pht hnh vi phn mm ci t trn a cng. Khch hng Turnkey ch cn thc hin theo cc th hng dn ci t c cung cp vi h thng ca h v b qua tt c cc phn trong hng dn ny c lin quan n ci t.Trc khi my tnh chng ta c th thu thp d liu, bn cn phi lm nh sau: 1. Ci t v cu hnh cc thnh phn phn cng my tnh. 2. Ci t v cu hnh phn mm h iu hnh. 3. Ci t v cu hnh phn cng thu thp thng tin v d liu. a) IEEE, ni tip, hoc my ch u cui.b) 900 / NCI giao din, 600 Series LINK, hoc giao din Softlink.c) Analog cp tn hiu.4. Ci t v cu hnh phn mm TotalChrom. Nhng iu cn chun b ci tThi gian ci t s khc nhau. Bn c th phi nh n s tr gip ca nhn vin Perkin Elmer ci t Total Chrom. Trong khi ch i bn nn lm nhng vic sau: Gii nn tt c thit b, c phn cng v phn mm. Cn phi c b tr li cp. Cung cp khng gian thit b cn thit cho h thng d liu. Hy chc chn c ngun in y v khng gian sch s, r rng, v an ton. t giao din A / D v / hoc m-un LINK nhng v tr mong mun. Hy chc chn rng bn c cc dy cp cn thit. Chng bao gm cc ng dn tng t, my in cp, IEEE / cp ni tip, ng dy dn t xa, Cc dn pht hin v bt u dn t xa cho cc thit b PerkinElmer ca bn kt ni bi cc k s dch v PerkinElmer. i vi dng c non-PerkinElmer, xin vui lng c nhng tn hiu kt ni vi cp xon i cho kt ni vi giao din 900 series. Chng ti khng th m ln thit b ca nh cung cp khc.Cn phi c mt bn sao ca my tnh ca bn v / hoc hng dn s dng h iu hnh. Ngoi ra, c hng dn s dng v a cho phn mm ca bn th ba, pht hin, my tnh, card m rng, v thit b ngoi vi. Nu chng ta mua iu khin thit b LINK cho thit b phn tch khc PerkinElmer, chng ta c th cn phi thay i cc thit b ny trc ln ci t. K s dch v hoc i din bn hng ca bn s c chi tit v nhng thay i ny. Sao lu bt k d liu cha c lu vi mt mng hoc a sao lu trc khi ci t. Nhng g cn cho ci t v tr gip:TotalChrom Application Managers Guide hng dn y v ci t v cu hnh. Nhn phm F1 c tr gip. Nu mt cu hnh c ci t ng Micrsoft Windows th ch mt 1 pht ci t Total Chrom.Nu gp cc vn khc bn c th gi v hotline: 1-800-762-4000 3. Lm th no c hiu qu cao v nhanh chng? Ngi dng mi vo mt h thng sc k d liuHng dn TotalChrom ca ngi s dng cung cp mt ci nhn tng quan. Xin vui lng, xem li cc phn trc khi chng ta bt u s dng TotalChrom. Cng vic ca chng ta s d dng hn nhiu. Thc hin ci t dch v t chnChng ta nn xem xt tt c cc thng tin trong gii thiu chng ca Hng dn TotalChrom ca ngi s dng dch v trc khi PerkinElmer i din ci t h thng. Trc khi i din dch v ca bn i, bn nn hiu nhng iu c bn ca vic to ra mt phng php, mt thit b cu hnh, v thu thp d liu khi chy. S dng TotalChrom Users TutorialKHNG xa cc tp tin v d vn chuyn vi TotalChrom khi bn ang thc hin vi hng dn. y l nhng hu ch s dng nh x l s c tr nu cn thit trong tng lai. Tm hiu cc vn c bn u tin TotalChrom c nhiu tnh nng v tnh linh hot m nhiu phng th nghim khng bao gi nhn ra tn ti. Bit ni Tr Gip TotalChrom c m rng tp tin tr gip trc tuyn (F1) cho mi phn mm m-un. Ngay sau khi phn mm c ci t, lm quen vi cc ch tr gip, lm th no tm kim thng tin tr gip, v lm th no tm kim nh ngha. TotalChrom thi quen ha cung cp v s cch xem, chnh sa v thao tc d liu. Thu thp d liu chyChc chn rng chng ta hiu bit v cch d liu, phng php, trnh t v cc tp tin c chnh sa, lu, v t tn trc khi chuyn.By gi chun b mu phn tch bn ngoi. Sau khi thc hin ci t, thit lp phng php phn tch tiu chun bn ngoi, tn thnh phn v hiu chun cc phng php ca bn theo cc hng dn trong sch hng dn ca ngi dng. Tim iu khin cho n khi bn cm thy thoi mi vi kt qu ca bn. Lp li qu trnh vi mt hot ng tiu chun ni b.

4. Nhng vn xy ra C gng tr li cc cu hi sau:H thng ca chng ta t nht l p ng yu cu ti thiu nh nu trong TotalChrom Ghi ch Pht hnh? Nu khng, chng ta s c vn . y thc s l c bn yu cu i vi cc phin bn ca phn mm.Chng ta kim tra cc ti liu? Kim tra cc phn ca ti liu gii thch cc lnh v th tc chng ta ang s dng m bo rng chng ta ang hot ng phn mm mt cch chnh xc. ng qun cc tp tin HELP (bng cch nhn phm F1)Chng ta thc hin cc th tc qun l tt trn h thng ca bn? bao gm vic v sinh my v c nh cc dy cp.Bn ang gp phi thng bo li c th? Xc nh xem nhng sai st n t my tnh, h iu hnh DOS, Windows, hoc cc phn mm sc k, v vit xung hoc lm cho mt bn in mn hnh chnh xc nhng g c thng bo cho bit. Bn nn truy cp vo h thng li ca Perkin Elmer.L vn lp li, hoc n c th c nhn i trn mt h thng khc? C bn gii quyt phn mm v phn cng b n l bit chnh xc khi chng xy ra v nhng g cc iu kin m dn n xut hin ca chng. Nu bn c nhiu h thng TotalChrom c sn, kim tra xem vn xy ra trn cc h thng khc. Nu khng, iu ny c th ch ra mt vn h thng v s gip tp trung cc n lc x l s c. Vn c th c nhn i vi cc tp tin d liu th nghim i km vi h thng? Nu khng, iu ny c th ch ra mt vn vi cc tp tin mi m bn to. Bn c trn mng? Xem nu vn vn cn tn ti khi bn ang b ngt kt ni t mng loi tr mt vn mng.iu g thay i? Hy hi tt c nhng ngi s dng hoc duy tr ca bn h thng cho d bt c iu g c thc hin n m bn khng bit v. C ai ci t mt gi phn mm mi hoc trnh iu khin c th l can thip C ai vo ca m lm thay i TotalChrom hoc Windows tp tin cu hnh t ln cui bn vn hnh h thng? C ai thay i th mc cho php ca ti? C mt khu ngi dng v / hoc qu trnh TotalChrom ti khon ca ti ht hn? iu g xy ra cho vn hoc ngh ca ti? Nh cc vn v xut lin quan n hot ng phn mm, chc nng, tnh nng, hoc ti liu c bo co ca ngi dng ng k, h tr phn mm bt u mt phn mm iu tra. Cuc iu tra ny bt u vi mt tm kim cho d vn ny c bo co trc y. Nu c, bn s c thng bo v phn gii. Nu mt vn c khng c bo co, phn mm h tr s c gng lp li kinh nghim ca bn v chun c h tr h thng my tnh bn ra ca PerkinElmer. Nu vn c th c nhn i bi phn mm h tr, v n nm trong phn mm ca chng ti, mt Phn mm Bo co iu tra (SIR) s c chun b v chuyn tip n PerkinElmer B sn phm, cng nh pht trin phn mm cho cc hnh ng tip theo.C g Vn ca ti khng th gii quyt ngay lp tc? Chng ti cn c thng tin chnh xc v tnh trng my ca bn a ra hng gii quyt ph hp.5. Nng cp v cp nhtC hai loi nng cp: nng cp sn phm v nng cp phn mm. Nng cp sn phm khch hng c la chn mua vo cc dng sn phm PerkinElmer vi cc tnh nng ti thiu, sau chuyn n nhiu sn phm y tnh nng. Nng cp thng gn lin vi nhu cu chin lc ca s pht trin phng th nghim c quyt nh bi ngi qun l phng th nghim v k s bn hng PerkinElmer.V d, bn c th mua mt sn phm nng cp ln TotalChrom t phn mm sc k Turbochrom Professional ca chng ti. Chng ti ch ni n nng cp sn phm y phn bit vi nng cp phn mm. Nng cp phn mm l phn chnh ca thit lp sn phm kt hp vi ci tin phn mm hoc s dng nn tng mi, h iu hnh, giao din ngi dng. TotalChrom v6.2 l mt phn mm nng cp t phin bn Turbochrom Professional v4 Cp nht phn mm khc nh th no?Nhng ci tin nh, thay i tnh nng, hoc bn sa li l kt qu khi ngi dng s dng vi cc phn mm c thc hin nh k cho cc m sn phm. Cc ci tin v sa li c tch ly v thng qua th nghim, chng ti c th pht hnh mt cp nht phn mm.Bn cp nht khng c ci tin nhng tnh nng chnh thng kt hp vi mt bn nng cp, nhng thng c mt lot cc ci tin v sa gip thc hin thao tc d dng hn. Mt phin bn cp nht c th hin bi s thay i cc ch s th hai ca m phin bn (v d; TotalChrom v6.2 n TotalChrom v6.x). Chnh sch v nng cp phn mm v cp nht ca PerkinElmer l g?Nng cp phn mm v cp nht s c cng b trong mt bc th gi cho ngi ng k s dng. Bc th ny s t vn cho bn v cc th tc lm theo v nhng g lin quan n chi ph c c giy php mi. Nu bn cha ng k, bn s khng nhn c thng bo v nhng sn phm mi.Theo quy nh chung, n c li cho ngi dng chuyn n phin bn hin ti ca cc phn mm. Bn cnh thc t rng vic pht hnh hin nay lun lun cha nhng ci tin v sa cha mi nht theo yu cu ca ngi s dng, phin bn c tr nn kh khn hn h tr v chng c thay th. Mt pht hnh bo tr l g?Nh chungs ta bit, pht trin phn mm l mt qu trnh ch ng. Trong khi bn c th s dng v1.1.1, Software Support c th c th nghim v1.1.2, trong khi Software Quality Assurance c th c th nghim v1.1.3, trong khi Software Development c th c m ha v1.1.4. Nu Software Support khng pht hnh v1.1.2, hoc nu v1.1.3 khng qua th nghim Software Quality Assurance, bn c th khng bao gi nhn thy cc phin bn. Bn c th ngh rng chng ta b qua mt phin bn hoc t nht l b qua cho bn bit v n, nhng thc t c th khng bao gi lm n pht hnh.Tuy nhin, nu v1.1.2 c chp thun cho pht hnh, chng ti c th a ra mt pht hnh bo dng (MR). Mt MR c xc nh bi mt s thay i trong th ba ch s ca m phin bn phn mm (v d; v6.1.0 v6.1.2). MRs thng ch cha cc bn sa li. Khng c thay i ti liu c thc hin.MR sn c v thng tin t hng c th nm trong khu vc h tr phn mm ca trang web ca chng ti (http://las.perkinelmer.com/). Bn cng c th nhn c thng tin ny t i din h tr phn mm ti a phng. B phn h tr s ch gi MRs cho nhng ngi dng c ng k cho cc phin bn ca sn phm m MR c to ra. Bn bo tr ph Thnh thong, k s phn mm c th pht hnh mt a bo dng c cha mt vi bn sa li cho mt m-un chng trnh c th c gi tr gii hn trong ton b cc c s ngi dng. Pht hnh bo dng b sung (SMRs) ch c pht hnh khi mt vi vn c th c c nh m khng nh hng n cc module khc trong phn mm. M SMR s c cung cp trn trang web PerkinElmer truy cp trc tip bi ngi s dng.Tm ttChng ti hy vng bi gii thiu ny c hu ch. Tt c chng ti trong Software Support mun c tri nghim ca bn vi cc sn phm ca chng ti v ton b i PerkinElmer, thnh cng trong nhiu nm. Xin vui lng cho chng ti bit lm th no chng ti c th gip nhiu hn, cho d l mt tnh nng m bn mun thy trong cc phn mm, hay mt chng trnh h tr bn mun thy chng ti trin khai trong tng lai.II. Hng dn phn 11. iu hng trong TotalChrom Chng ta truy cp vo cc chc nng khc nhau trong TotalChrom t ca s iu hng. Cch thun tin nht bt u iu hng l t ca s "TCWS_Links" (hoc TCCS_Links cho TotalChrom Client / Server), c to ra trong qu trnh ci t. m ca s nh hng: 1. Nhp vo nt Start, Programs, sau nhp vo TotalChrom Workstation Ver6.3 hin th ca s Lin kt. Double-click vo biu tng TCNav trong ca s ny

Hp thoi ng nhp xut hin:

Lu : Chng ta phi ng nhp vo mt ti khon c kch hot quyn qun l truy cp. Nu bn c thc mc, tham kho ngi qun l h thng ca bn.2. Enter your Logon User Name and your Password. Then choose OK.

Ca s iu hng xut hin:

Cc ca s iu hng l mt giao din ha ca cc chc nng chnh trong TotalChrom. iu hng c bn lnh vc chnh: Title Bar v Menu Bar trn u trang. Cng c Selection Panel bn tri.Nt chc nng trung tm ca mn hnh.Lu : Mn hnh ny cho thy cc tu chn TCPublisher c la chn. thay i, xem li v ng , chn TotalChrom Review & Approve trong hp thoi Reporting Options. iu ny c hin th bng cch chn Reporting t Build>Configuration>User> menu.Status Bar di cng ca ca s.Chng ti s dng cng c la chn bng iu khin v mt s nt chc nng trong hng dn. Thu thp File d liu u tinTrong phn ny ca hng dn, chng ta s: S dng Quick Start Xy dng mt phng php nhanh chng bng cch ch nh cc cng c thng s.Xy dng mt danh sch vial Thu nhn d liuiu chnh thi gian thc ca cc im d liu khi chng c thu nhn.Xem li cc bn in ra. S dng Quick Start thu nhn d liu.Cc ty chn Quick Start cho php bn thu thp d liu khng cn pht trin mt phng php ton din. Vi Quick Start, bn ch nhp cc thng s cn thit cho vic thc hin mt hot ng.Khi ng nhanh c ch khi bn ln u tin bt u s dng TotalChrom, nu bn ang thc hin "kho st" chy trn mt loi mu m bn khng phn tch trc y, hoc khi bn ang pht trin thit lp cng c phn tch. bt u qu trnh ci t thu nhn d liu:1. Chn thit b bn mun s dng thu thp d liu t bng iu khin la chn cng c trong iu hng (bn s s dng cng c ny trong sut ton b hng dn).

2. Click vo ci t trong bng iu hng

Hp thoi ci t cng c xut hin

3. Trong hp thoi Setup Instrument, chn Quick start khi Setup Type.

4. Nhn vo biu tng th mc bn cnh hp vn bn Raw File Path nh trn. Xut hp thoi hin TotalChrom Path- Select:5. Trong hp thoi TotalChrom Path-Select, chn ton ng th mc C:\penexe\..\examples bng cch nhn i cho n khi ng dn y s c hin th trn danh sch cc th mc. Chn OK.6. Thc hin cc quy trnh tng t cho hp vn bn Result File Path.7. nhp mt Base File Name, nhn vo nt bn phi ca hp vn bn.

Xut hin hp thoi Default Base Names.

8. Trong hp thoi Default Base Names, chn NONE cho Separator v loi TEST trong file Base File Name Hp thoi ny tho lun v tn tp tin Tokenized (mt tnh nng mi cho phin bn 6.2). Vui lng tham kho Users Guide m t chi tit v ty chn ny. Tt c cc file c lin quan n phng php ny - file nguyn gc, kt qu, phng php v trnh t - s s dng base file name.9. Nhn OK ng hp thoi Default Base Names.10. hp kim tra " Suppress processing" v " Suppress reports/plots" c kim sot nh trong mn hnh trn.Chng ti mun x l tp tin d liu ngay lp tc sau khi n c thu thp, v chng ti mun bo co c in sau cng.11. Khng c nhp vo nt OK no.2. Xy dng mt phng php nhanh xy dng mt phng php nhanh1. Trong hp thoi Setup Instrument, bm vo nt bn tri ca Quick method (phng php nhanh).

2. Chng ta c th nhp bt k thng tin m t v phng php trong ca s ny hoc bm vo NEXT n trng. Do Not Select Audit Trail (Khng Chn Kim ton Trail).Ca s tip theo xut hin l hp thoi Instrument Notes3. Chng ta c th nhp bt k thng tin c th c vo hp thoi ny hoc chn t cc mu n th xung vi mt trong cc mu thit k sn. Chng ta cng c th chn trng, bng cch bm vo NEXT hin th hp thoi Data Acquisition. Cc thng tin hin th trn tab Data Channels khc nhau ty thuc vo cng c chng ta chn lc u ca th tc ny. iu ny c th hin trong hai con s sau y:

Data Channels tab for a 900/NCI Series Interface

Data Channels tab for a LINK-controlled instrument 4. Trong Data Channel (knh d liu) bn di, hy chn knh (A, B, hoc Dual) ph hp vi cch pht hin ca u do (detector) c kt ni vi giao din. 5. i vi mt cng c LINK-controlled, chn mt ngun d liu t danh sch th xung (mt s dng c khng cung cp mt s la chn). HOC i vi 900/NCI Series Interface, chn phm vi in p cn thit tng ng vi lng pht hin t detector.

6. Chn mt ty chn Set Data Rate.

Nu chng ta bit cch ly khong rng v khong hp nht ca peack lc ban u ca n, chn By peak width at Base (chiu rng peack) v nhp s giy trong khung k bn. TotalChrom s s dng tnh ton t l ly mu ti u cho peack . HOC Capillary GC = 20 pts/sec HPLC or IC = 5 pts/sec Packed column GC = 2 pts/sec

Chn By sampling rate (tc ly mu) v chn hoc nhp mt tc (ty thuc vo thit b). Tc ly mu ngh l: 7. Chng ta mun tit kim thi gian hon thnh qu trnh chy bng cch s dng thi gian ch v thi gian chy lu tr.i vi mt cng c LINK-controlled, chn Store all data from run. (Lu tr tt c d liu t qu trinh chy."

HOC i vi 900/NCI Series Interface, li Delay time (thi gian ch) thit lp l 0, v nhp Run time (thi gian chy) tng ng vi chiu di ca qu trnh chy.

8. Chn NEXT hin th u tin trong mt lot cc hp thoi i vi cng c chng ta chn.9. Hon thnh mi lot cc hp thoi bng cch s dng cc gi tr thch hp cho mu thit b.Lu : Nhn F1 khi mt hp thoi c m ra hin th cc tr gip trc tuyn cho hp thoi .

Cc hp thoi hin th trong phn cn li ca lot bi ny ph thuc vo cng c m ta chn. Chng ta s khng nhp bt k thng s x l cho phng php nhanh. N thng d dng hn thit lp cc thng s sau khi thc t bng cch s dng phng php bin tp ha. Chng ti s s dng chc nng trong phn 2 ca hng dn. Khi chng ta t n hp thoi Component (thnh phn), l mn hnh cui cng trong lot cho tt c cc loi cng c, nhp vo Finish. Khi chng ta hon thnh hp thoi Quick Method, Method Summary, Component List (phn danh sch), hoc kt hp c hai mn hnh s c hin th cho thy cc thng s ban u m chng ta thit lp.

lu Phng php nhanh1. Chn Save t menu File lu phng php. Cc phng php nhanh c lu bng cch s dng tn tp tin m chng ta nhp trong hp thoi Setup, cng vi phn m rng .MTH (v d, TEST.MTH trong hng dn ny). 2. Chn Exit t menu File tr v ci t hp thoi Setup Instrument. bit thm thng tin v cc thng s thit b, xem Chng 6, Developing Instrument Parameters in the Method, trong hng dn ngi s dng TotalChrom. bit thm thng tin v phng php nhanh, xem Using Quick Method trong Chng 12 ca hng dn ngi s dng TotalChrom. Mc d n khng phi l cn thit cho tt c cc cu hnh thit b, cng vic tip theo chng ta s thc hin trong hng dn ny c xy dng mt danh sch l xc nh cc mu c tim. 3. Xy dng danh sch l xy dng mt danh sch l1. Trong hp thoi Setup Instrument, bm vo nt Vial list

M ca s Vial List th ca s Sequence Information hin ra:

2. Nu chng ta s dng mt LINK-controlled LC autosampler, chng ta phi ch ra cc loi khay ng.a. Chn Global Parameters t menu Change hin th hp thoi Global Parameters

b. Chn loi ci t trong danh mc Tray.c. Nhp chut vo OK.3. Trong ca s Sequence Information, chn u tin trong ct Vial bng cch nhp vo n.

4. i vi h thng ly mu t ng LINK-controlled, nhp s l ni bn mu nm. i vi tt c cc cu hnh cng c khc, li cc mc mc nh. 5. Khng nhp bt k l c. Thay vo , xa cc hng cn li, t 2 n 5. 6. Lm ni bt dng th 2 sau nhp vo menu Edit. Ch rng chng ta c nhiu la chn cho cc thao tc hng trong bng tnh.

7. Chn Delete t menu Edit. Dng 2 s b xa v di chuyn ln hng 3-5 trong bng tnh. 8. Xa 3 hng cn li theo cch tng t. Chng ta hon tt danh sch l v sn sng lu li v bt u chy. lu danh sch l: 1. Chn Save t menu File. Danh sch l c lu di dng mt, bng cch s dng c s tn tp tin m bn nhp vo hp thoi Setup, cng vi phn m rng .SEQ (TEST.SEQ trong hng dn ny). 2. Chn Exit t menu File ng ca s danh sch l v tr v hp thoi Setup Instrument. 3. Chn OK trong hp thoi Setup Instrument khi to cc cng c v tr v Navigator. By gi chng ta hon thnh tt c mi th m chng ta cn c c d liu.Bn sn sng bm tiu chun ca bn v thu thp d liu.

Injecting your Standard and Acquiring Data (Tim mu chun v thu thp d liu)By gi chng ta hon thnh cc th tc ci t v cng c c khi to, khung Status trong Navigator ch ra rng thit b sn sng.

Bt u thu thp d liuCch chng ta bt u chy ph thuc vo cc thit b ca v lm th no m chng c kt ni. i vi h thng ly mu t ng LINK-controlled, bm vo nt Run trong Navigator sau chn Start Run t menu.

HOC i vi mt A/D kt ni vi mt thit b sc k vi mt b phn ly mu t ng, A/D c th c chuyn n bt u ly mu khi mu t ng tim.HOC i vi mt thit b m khng c b phn ly mu t ng, bm mu chun vo sc k. Nu sc k c kt ni vi LINK, nhn bt u trn sc k. Nu sc k c kt ni vi mt A/D, chng ta c th phi nhn nt khi ng trn bng iu khin pha trc ca n. Khi bt u chy, Status ch ra rng thit b ang hot ng v ly mu.4. Xem Real-Time Plot Nt Real-Time Plot cho php hin th mt biu v d liu khi n ang c hin th bi mt giao din. M ca s Real-Time Plot: Trong Navigator, nhp chut vo nt Real-Time m ca s Real-Time Plot.

Cc thng s cho mn hnh hin th ban u n t hp thoi Real-Time Plot Scale m chng ta hon thnh trong phng php nhanh bng cch chp nhn mc nh ca n. C mt s iu m chng ta c th lm t ca s Real-Time Plot. Chng ta s th nghim vi vic thay i mn hnh hin th t menu Options. Mn hnh thay i ny l tm thi, ch nh hng n mn hnh hin th hin ti; chng khng thay i cc thit lp trong phng php ny v chng khng nh hng n d liu.

a) Phng to trong Real-Time PlotZoom In (phng to) v Zoom Out (thu nh) cc lnh trong menu Options ca ca s Real-Time s cho php chng ta phng to v lm gim tm nhn ca chng ta v biu thi gian thc nh cc im d liu ang c thu thp. Phng to v thu nh ca biu thi gian thc: 1. Trong ca s Real-Time Plot, hin th menu Options.

2. Chn Zoom In (cho cc knh m chng ta mun xem) m rng biu . 3. Chn Phng to mt ln na cho n khi ln nh chng ta mun. 4. Chn Zoom Out cho n khi xem biu l khi phc li kch thc ban u ca n.b) Qut biu Lnh Rescale trong menu Option cho php thay i cc thng s biu trong khi d liu c thu thp. Thay i cc thng s m rng quy m trong biu thi gian thc: 1. Trong ca s Real-Time Plot, chn Rescale t menu Options m hp thoi Rescale.

2. Gp i gi tr cho Full Scale, sau chn OK.3. Cho php thi gian kt thc v cc d liu c x l. 4. ng ca s Real-Time Plot, chn Exit t menu Instrument tr v Navigator. Lu : Chng ta s khng s dng li bt k tp tin c to ra trong phn ny ca hng dn. Trong phn 2, chng ta s s dng tp tin mu c chuyn ti TotalChrom. III. Hng dn phn 21. Ti u ha phng php s dng chnh sa ha Trong phn ny ca hng dn, chng ta s s dng mt s tnh nng c bn ca phng php chnh sa ha: Thay i mn hnh hin th th. iu chnh cc thng s pht hin cao im. To mt danh sch thnh phn.Xem d liu di dng ha gip bn xc nh nh ang c pht hin mt cch chnh xc, nu ng c s ang c t ng cch, v nu thi gian lu gi d kin v ca s tm kim cho cc thnh phn c xc nh chnh xc nh. bit thm thng tin v phng php chnh sa ha, xem Chng 10 trong hng dn ngi s dng TotalChrom.

M phng php chnh sa haChng ti s bt u bng cch m Phng php ha bin son v hin th mt sc k m c ti u ha. m phng php chnh sa ha v ti mt tp tin: Trong Navigator, nhp vo nt Chnh sa ha. M ca s phng php chnh sa ha cng nh m mt hp thoi chn tp tin s hin th mt danh sch cc file d liu th.

Chn "Examples" th mc t danh sch th xung ng dn TotalChrom nhanh hoc t danh sch cc th mc. T danh sch cc tp tin trong th mc "Examples", chn halo003.raw. Chn Open

Mn hnh hin th mc nh cho thy mt sc k ang hot ng trong khu vc lm vic chnh ca ca s v mt sc k tham kho trn n. Tn ca tp tin d liu th s xut hin trong thanh trng thi v tn ca cc phng php c s dng x l d liu xut hin trn thanh tiu .

Trong cc bc sau, chng ta s khm ph mt s trong nhng cch m bn c th ti u ha mt phng php t c kt qu nh th ny. thit lp li cc thng s phng php mc nh: Chn New t menu File. Cc thng s mc nh phng php c p dng cho cc tp tin halo003.raw.

Trc khi chng ta thay i bt k thng s phng php, chng ti s tri nghim vi vic thay i cch thc thng tin c hin th sc k. Thay i hin th thC mt s cch thay i mn hnh hin th th. Chng ti s th nghim vi hai trong s trong phn ny ca hng dn.Chng ta c th s dng chut thay i mn hnh hin th ni bt ca cc khu vc ca ct truyn m bn mun m rng. Chng ti s lm iu ny t sc k tham chiu; bn cng c th lm iu t sc k lm vic. chn v thao tc mt phn ca sc k tham kho: Chn mt phn ca sc k tham kho nh trong v d di y bng cch gi nt chut tri v ko chut phc tho khu vc. Th nt chut.

Sc k lm vic s m rng ph hp vi vng c nh du trong sc k tham kho. Ch rng cc sc k lm vic ph hp vi vng c nh du chnh xc, do , bt k nh c chiu cao y , bn khng chn l ct ngn.Di chuyn con tr (mi tn) vo khu vc c nh du trong sc k tham kho. Con tr chut thay i thnh mt con tr crosshair dy. iu ny cho thy rng bn c th di chuyn vng la chn. V tr con tr bt c ni no trn sc k lm vic, sau nhp vo nt bn phi khi phc li sc k vi quy m ban u ca n.

Nu bn di chuyn hnh ch nht nhiu hn mt ln, nhp vo nt chut phi n khi tr v sc k vi quy m ban u ca n. Mt thng bo s c hin th khi khng c m rng hn. rescale hin th th mt khu vc quan tm: Vi sc k tr li vi quy m ban u ca n hin th ton b chy, chn Rescale Plot t menu hin th.

Trong hp thoi Rescale Plot, lm cho thay i cc gi tr sau:a. Thi gian kt thc thay i 0,55.b. Thay i Offset 0.c. Thay i hon ton quy m 10.Nhp vo OK v li cc th s dng cc gi tr mi.Mn hnh ca bn s trng ging nh v d di y:

bit thm thng tin v vic thay i mn hnh hin th, xem "Changing Display Options" trong Chng 10 ca hng dn ngi s dng TotalChrom. Diu chnh pht hin nhK t khi pht hin cc thit lp mc nh nh rt nhy cm, qu nhiu nh c pht hin trong sc k mu ca chng ti do nhiu ng c s. m bo rng TotalChrom pht hin nh nh, nhng khng gii thch nhiu c bn nh nh, chng ta phi tnh ton nhiu c bn v thit lp nhiu v khu vc ngng b qua n. tnh ton ngng nhiu ti u:Chn ngng ting n / Din tch t menu Process.Cc thng bo trn thanh trng thi cho bn phc tho nhng phn ng c s m bn mun s dng tnh ton mt mc ngng mi.Chn ton b ng c s bng cch gi nt chut tri v ko chut phc tho khu vc.

Th nt chut hin th nhiu / Khu vc hp thoi Threshold.

TotalChrom tnh ton ngng ti u cho (khong 80 v 400).Click vo OK ti x l cc d liu v v li cc th s dng cc ngng ngh. Nh trong v d di y, ting nhiu v khu vc cc gi tr ngng mi loi b cc nh c.

Nhn vo nt chut phi tr li sc k vi mc trc m rng (kch thc y sc).S lng cc im d liu trn mt nh pht hin cao im. Nu bn c qu nhiu im trn nh cao, bn c th gim t l ly mu cho chy trong tng lai (bn khng th thay i t l ly mu d liu khi thu thp c) hoc lm tng yu t t nhm lc ra d liu khng cn thit t cc tp tin hin c.iu tip theo chng ti s lm ti u ha pht hin nh cao l iu chnh cc yu t t nhm. thit lp mt yu t t nhm mi: Chn Tn s mu / t nhm yu t t menu Process. Cc thng bo trn thanh trng thi cho bn phc tho nh hp s dng tnh ton. Xem tng b rng ca nh u tin (khng chn phn tailing) bng cch gi nt chut tri v ko chut

Th nt chut hin th cc nhm yu t hp thoi.

TotalChrom tnh ton mt yu t t nhm c ngh ca 2 trong v d trn (so vi cc yu t t nhm c ca 1 t phng php mc nh). Vi ty chn t nhm yu t c la chn, chn OK ti x l cc d liu v v li sc k bng cch s dng yu t t nhm mi. S thay i sc k ca chng ti l tinh t. Khi bn ti u ha mt phng php trong cng vic hng ngy ca bn, nhng thay i c th nhn thy c th l ng k.

By gi bn ti u ha cc phng php pht hin nh v hi nhp. Bc tip theo l phi c TotalChrom gn tn cho mt s nh v tnh ton s lng (hoc nng ) ca cc nh ni. lm iu ny bn phi to mt danh sch thnh phn. To mt danh sch phn Trong thi gian pht hin cao im, TotalChrom gn mt s trt t cho mi nh pht hin. iu ny c gi l danh sch nh. Danh sch thnh phn, mt khc, l mt danh sch m bn to ra trong cc phng php xc nh mt nh nh mt thnh phn c th. Chng ti s to ra ba thnh phn trong bi tp ny. thm cc thnh phn nh duy nht:Chn Edit Components t the Calibration menu.

th c thu hp nhng ch cho cc Chnh sa cc thnh phn hp thoi:

Click vo nh 5 (thi gian lu 2,81) di chuyn hnh ch nht la chn n t nh u tin.Chn Ref. check box nh l mt thnh phn nh tham chiu cho cc thnh phn khc.Cao im tham chiu c s dng iu chnh thi gian lu gi d kin trong xc nh thnh phn.Chn hp kim ISTD _ _ nh mt tiu chun thnh phn ni b cho cc thnh phn khc.Cc tiu chun ni b iu chnh cc phn ng i vi bt k s thay i trong khi lng tim. Tn thnh phn, nhp Chlorotoluene.Tng ca s tm kim l tng ca cc ca s tuyt i v tng i, p dng cho c hai bn thi gian lu gi d kin ca cc thnh phn.Chn nh cao nht Tm trong hp ca s kim tra.iu ny s xc nh cc nh ni cao nht tch ra bn trong ca s tm kim nh cc thnh phn c ch nh.iu ny hon thnh mc cho nh cao ny. Hp thoi ca bn s ging nh v d di y

Chn Trc di chuyn vng la chn n nh 4 (nh cao nht trc 5).

i vi tn thnh phn cho 4 nh, nhp 1,2-Dichloroethene. T c tham kho v ISTD danh sch th xung, chn Chlorotoluene.Thay i mc Absolute Window 5 ( ca s tng i thit lp 3).Chn tm nh cao nht trong hp ca s kim tra.Hp thoi ca bn s ging nh v d sau:

Chn Trc di chuyn vng la chn n 3 (nh trc nh 4).Tn thnh phn cho nh 3, nhp Chloroform.T c tham kho v ISTD danh sch th xung, chn ChlorotolueneThay i mc Absolute Window 5 ( ca s tng i thit lp 3).Chn nh cao nht Tm trong hp ca s kim tra.Chn Tr v ng hp thoi

Sc k ca bn by gi c dn nhn vi ba thnh phn m bn va to.2. S dng phng php bin tpTrong phn ny chng ta s: Thit lp cc thng s hiu chun Thc hin hiu chun th cngChng ta s s dng phng php bin tp xy dng mt phng php hon chnh. N bao gm cc thng s khng c sn trong phn ho ca phng php bin tp hoc khi chng ta cn pht trin mt phng php nhanh khc. Thit lp cc thng s hiu chunPht trin cc thng s hiu chun trong mt phng php:1. Chn phng php t menu Other chuyn sang Method Editor v hin th cc thng tin tm tt cho tng phn ca phng php.

2. Chn Edit component t menu Components m ca s Components

Ca s c hai cp ca Method Editor

Tn ca cc thnh phn m chng ta mun phn tch xc nh bn tri ca s. thn tin cc thnh phn c la chn nm bn phi. Chng ta s thay i cc thng s c sn bng cch thm vo cp hiu chun cho mi thnh phn.3. nh du Chloroform , sau chon th Calibration m cc hp thoi cc thng s hiu chun cho thnh phn .

* Ghi ch: cc thng s i y l gi tr mc nh ban u ca TotalChrom. Nhng gi tr ny c th khc nhau trn cc my khc.

4. Chn Use curve trong tu chn Calibration Type.iu ny to ra mt ng cong hiu chun da trn d liu thu thp t cc tiu chun mu.5. Chn Area trong tu chn Response.iu ny cho bit TotalChrom dng hiu chun v nh lng s dng din tch peak.6. Chn Point to Point khi thy Curve Fit Type. l trung bnh ca tt c cc s liu ti to v d liu tr v ti mi cp hiu chun ly c mt im. Mi cp im s c ni vi nhau bng mt ng thng phn khc to thnh mt ng cong hiu chun.7. Chn None cho c Scaling v Weighting.8. Under Origin Treatment, nh du vo Include check box.iu ny s thm vo im (0,0) hiu chun (s 0 tr v 0).Phn tip theo m t quy trnh lm th no thm mt cp hiu chun trong phn cui ca bng h thoi.

9. Chn Level v nhp Level 1 cho tn ca Level.Nn g tn cp l ch v s.10. Chn hp thoi vn bn Amount v nhp 30.Gi tr din tch s c tnh ton v in vo khi chng ta chy ng hiu chun.11. Nu c nhng mc mc nh hin th trn danh sch (bt c g ngoi Level 1 c nhp vo) chn tng mc v n delete xo n. y l mt quy trnh xo mt cp hiu chun.Khi nhp vo hp thoi Level 2, mn hnh s hin th ging nh v d sau:

12. Chn Next hin th thng tin hiu chun cho 1,2-Dichloroethe.13. Chn cc mc tng t nh Chloroform cho 1,2-Dichloroethene:Calibration Type = Use Curve Response = Area Curve Fit Type = Point to Point Scaling/Weighting = None Origin = Include Calibration level = Level 1 Calibration amount = 30Xo cc mc cp hiu chun mc nh, nu c.14. Chn Next hin thng tin hiu chun cho Chlorotoluene.15. Chn cc mc tng t cho Chlorotoluene ging nh lm vi cc thnh phn trc . Ngoi tr s lng hiu chun l 20 thay v 30:Calibration Type = Use Curve Response = Area Curve Fit Type = Point to Point Scaling/Weighting = None Origin = Include Calibration level = Level 1 Calibration amount = 20Xo cc mc cp hiu chun mc nh, nu c.16. Chn Apply, sau chn OK quay li ca s Method Summary.By gi chng ta hon tt vic thm cp hiu chun cho mi thnh phn. Chng ta c mi th chng ta cn thc hin hiu chun th cng.

Thc hin hiu chun th cngKhi t hiu chun mt phng php, bn ang to ra s ln hiu chun lp li. Mt ln lp li l mt ln phn tch ca ng chun hiu chun cho nhng cp hiu chun c bit. V n l mt cng ngh phan tch c o, nn bn c th lp li ly hiu chun trung bnh.Khi thc hin hiu chun th cng, chng ta xy dng danh sch cc files kt qu v levels hiu chun m chng ta mun hiu chun v cc loi hiu chun m chng ta mun thc hin.Thc hin hiu chun th cng1. Chn Cablirate t menu Components m hp thoi Manual Calibration.

2. Duyt n th mc Example, chn biu tng th mc mu vng bn phi hp thoi Selected File, sau chn file kt qu Halo003.rst t hp thoi File-Select.

Sau chn Open, y l tp tin chng ta xc nh nh mt tiu chun hiu chun.3. Chn Level 1, t menu ko th xung trong hp thoi Calibration Parameters Level.y l cp hiu chun ca file tiu chun.4. Chn Replace khi thy Type.Nu phng php ca chng ta c hiu chun, iu ny s thay th nhng ln hiu chun tn ti bng nhng ln mi.5. Chn Add thm th mc hiu chun vo danh sch Filename/Level/Cal Type cui hp thoi.Nu c nhiu cp hiu chun thi im ny, chng ta nn chn theo kt hp file, level, and type v thm n vo danh sch.6. Chn Identify peaks before calibrating nhng peaks kt qu c xc nh trc khi hiu chun.Mn hnh s hin th ging nh v d sau:

7. Chn OK thc hin hiu chun s dng thng tin trong danh sch Filename/Level/Cal Type v quay li ca s Method Summary.8. xem nhng hiu chun cp nht, quay li th Calibration bng cch chn Edit Component t menu Components, sau chn th Calibration.

Ch rng din tch c tnh ton v hin th pha di gc phi ca ca s.9. Chn OK quay li ca s Method Summary.10. xem ng cong hiu chun, chng ta c th chn danh sch Component t ca s Window chuyn sang mn hnh Method Summary.

Chng ta c th chuyn i gia Method Summary v Component List bng ca s nh v ln ca Method Editor bng cch chn vo gc trn bn phi ca s.11. Quay li ca s Method Summary, chn Window ri chn Method Summary.

Mc d c rt nhiu cch c th tu chnh mt phng php. Tip theo l phn lu li nhng g lm.

Lu phng phpLu mt phng php mi1. Chn Save As t menu File.2. Khi hp thoi Description m ra, nhp bt k thng tin no bn mun lu tr vi method file. Khng chn Audit trail.3. Chn OK.4. t tn method bng cch g tn nh File name.

Lu method ng dn (C:\penexe\\practice.mth).5. Chn Save lu method.Chng ta hon thnh ti u vic phng php ny v lu li cho vic s dng trong tng lai.Bc tip theo l to nh dng cho bi bo co s c in ra sau khi phn tch s dng phng php ny.

Xy dng nh dng bo coSau khi TotalChrom phn tch d liu ban u, n s dng file nh dng bo co khi in kt qu phn tch. File ny iu khin cc thng s bao gm bo co c in ra v xc nh thng tin xut ra nh th no trn giy.Chng ta s s dng Report Format Editor thc hin chc nng ny. Tn bo co. Thm, thay th, v xo ct bo co. Lu bo co s dng cho sau ny.3. To nh dng bo co mi1. Ca s Method Editor vn cn hin th, chn Report Format Editor t menu Other.Khi khng c nh dng bo co vi tn ny, TotalChrom s dng tn ca phng php m vi gi tr mc nh ci t mt bo co nh dng mi.

Vic u tin chng ta lm l thay i tn ca bo co.2. Chn Title t menu User Notes.3. Trong hp thoi Title, thay i tn mc nh sang TUTORIAL REPORT.4. Chn OK hin th li bo co vi tiu mi.Chng ta c th hin th hu ht cc hp thoi bin tp bng cch bm trc tip vo khu vc cn thay i (v d, bm TUTORIAL REPORT hin th hp thoi Title)Tip theo chng ta thm ct vo bo co5. xem danh sch tt c cc ty chn cho tiu ct, chn Report hin th menu.6. La chn Component Name t Report menu.

7. Trong hp thoi Component Name thay i s ct t 9 sang 3.8. Chn Insert/Add.Mt ct cho Component Name c chn vo trc ct Area. Phng ch tr nn nh hn bo co ph hp trn mn hnh, v mt phng ch nn s c s dng khi bo co ny c in.

By gi chng ta s thay i tiu ct bng cch thay th n vi la chn khc nhau.

9. La chn Adjust Amount t menu Report.

10. Trong hp thoi Adjusted Amount, thay i s ct t 10 sang 7.11. Chn Raplace Adjusted Amount c thm vo ct 7, thay cho Normalized Area.

Thay i cui cng chng ta s lm trc khi lu file l xo mt ct t bo co.

12. La chn Delete Column t Edit menu.13. Trong hp thoi Delete Column, nhp 9 cho ct b xo (Area/Height).14. Chn OK.Ch rng vi cc tiu ct t hn phng ch tr li kch thc ban u ca n.

15. Chn Save t menu File.16. Chn Exit t menu File quay li tm tt phng php.Khng ng ca s Method Editor.By gi chng ta ti u ho v lu li phng php thc hnh, chng ta s xy dng mt trnh t xc nh cho vic ti u ho phng php ny.

Trnh t c dng kim sot vic thu thp v phn tch d liu sc k mi ln tim mu.Vi mc ch ny, chng ti s xy dng mt trnh t ti x l nhng d liu c lu tr. lm iu ny, chng ti s s dng mu t v bng biu. Trong hu ht cc trng hp, tp tin .idx s c s dng cho vic ti x l d liu.4. S dng mu t Cc mu t n gin ha qu trnh xy dng trnh t bng cch cho php bn xc nh cu trc c bn ca n trong mt hp thoi.

Xy dng trnh t s dng mu1. Chn Method Editor, chn Sequence Editor.2. In the Sequence Editor window, choose New from the File menu

Sau hp thoi Global Parameters c m Ti Build Parameters chn tn thit b Instrument.Chn From template Build3. Vo Configuration

Chn nh hnh trn. Sau OK m hp thoi Sequence Template. Study: g tn Tutorial xc nh php phn tch c thc hin Method: Chn phng php c th theo ng dn C:\penexe\\practice.mth S dng phng php mi c ti u ha ti x l cc tp tin d liu th. Thay i Base file name thnh HALO cho Chanel ANote: HALO l tn ca mt nhm cc tp tin th c cung cp bi TotalChrom nh v d. Khng th s dng cc tp tin th m bn c c trong phn 1 ca hng dn t mi ngi dng c mt cch khc nhau.y l tn c s ca cc file kt qu s c lit k trong bng trnh t. Calibration: b chn Include calibration standards. Chng ti hiu chnh phng php bng th cng trong Method Editor.

Sample: + Chn 3 Number of samples. S mu phn tch trong trnh t.

+ Chn 1 # Injections per sample Click OK ng. Khi hon thnh cc mu t, TotalChrom xy dng trnh t. Sau c th xem v chnh sa cc d liu chui di dng bng biu. S dng bng biu trnh t Cc thng s trong mt trnh t c th c hin th di dng bng trong Sequence Information. S dng bng tnh l cch d nht chnh sa mt trnh t.

Bng trnh t ca bn nn c 3 hng, s lng mu m bn ch nh. Mi hng c

Cha thng tin v cc mu v cc gi tr s c s dng khi cc d liu th c x l li. xem cc ct pha sau v thng tin bn phi cun bng v bn phi Ct Method hin th tn ca cc phng php. Ct Report l tn ca tp tin bo co s c s dng to bo co. Ct Data xc nh cc tp tin c dng lp bo co. Khi bn xy dng mt trnh t c c th t ca cc injection, cm t ny s l tn c s ca cc tp tin c to. Cc thng s m bn khng thay i cha gi tr mc nh hoc khng c gi tr. Cc ct c hin th c th khc nhau ty thuc vo option c chn trong Global Parameters, Configuration hoc ngi dng nh ngha. Edit: khi pht trin danh sch vial trong Phn 1 ca hng dn. N cung cp nhiu la chn cho cc thao tc hng trong bng tnh. Change : thay i ng dn cho cc tp tin d liu TotalChrom bit phi tm chng trong th mc c sn ch khng phi trong sch hng dn. Chnh sa trnh t bng bng biu:1. Chn Path t Change m hp thoi Change Path.

Ending Row: chn 3 Path to change: nhp du * New path: Chn th mc Example

Change path for: chn Data

Bn s nhn thy rng cc ng dn c hin th trong ct d liu ch vo th mc Examples1. Click vo Printer th xung v chn mc i din cho printer m bn mun s dng.2. Lm tng t cho cc ct Plotter3. Chn Fill Down t Change sao chp cc mc t hng 1 n mi trong ct. Nhp phi vo cc v chn Fill Down cng c th thc hin iu ny

Lu trnh t nh l mt trnh t mi:1. Chn File/ Save as2. Khi hp thoi Description m ra, nhp thng tin ca tp tin trnh t m bn mun lu tr. Khng chn Audit trail. OK3. Trong hp thoi Save As. Nhp tn tp tin vo File name. Save trnh t bng ng dn C:\penexe\.\tn.seq4. Cui cng chn OK. 5. Qu trnh x l li mt tp tinh trnh t Mi ln c c d liu v lu tr n trong mt tp tin d liu th, c th x l d liu lp i lp li.

X l li cc tp tin trong mt chui1. Chn Actions/BatchBi v chng ti la chn chc nng Batch Reprocessing khi chng ta m mt trnh t, trnh t c cung cp nh l mc nh.

2. Chn ng dn d liu thc t trong th mc Examples directory ti Actual data path.Data Path l ni m d liu th v kt qu c lu tr. Nu s dng ng dn y cho cc tp tin d liu trong bng th t, m lm trong bi tp trc, bn c th trng field ny. Chn OK.3. Start analysis: Chn PEAK DETECTION4. End analysis: Chn REPLOT GENERATION5. Batch execution: Chn Local nu c my khch/my ch. Chn Interactive nu bn coa mng my tnh.

6. Chn Overwrite existing result files. Cui cng chon OK.Ca s Batch Reprocessing m, lit k cc tp tin liu s c x l li, chng l ba tp tin t trnh t.

7. Chn Start Reprocess menu bt u x l. bn cng c th bt u qu trnh Batch bng cch nhp vo mi tn mu xanh trn thanh cng c.Cc mc trong danh sch s b xa khi chng c x l. Report v Replot ca mi tp tin s c in mt ln d liu phn tch hon tt.

8. ng ca s Batch Reprocessing v tr v Navigator. chn Exit t menu File. bit thm thng tin v qu trnh ti x l, xem Chapter 15 of the TotalChrom Users Guide.6. Xem kt qu s dng kt qu x l li Khi Ti ch hon tt, bn c th xem kt qu s dng kt qu x l li. Xem file kt qu trong kt qu x l li

1. Chn Results Reprocess trn Navigator.2. Chn file Halo003.rst trong Examples. Open.

3. Thy rng tp tin Halo003.rst c cc thng s phng php p dng cho n m bn ch nh trong practice.mth to ra trong Graphic Method Editor.Bng cch s dng Print Preview, hoc nhn vo cc bo co c in, bn s nhn thy rng cc phng php x l c s dng cho cc tp tin d liu ny l phng php practice.mth m bn to ra.

Bo co in ra ca bn s ging nh bo co trn.7. Xem xt v ph duyt d liu Phn ny s bao gm ba ca s chnh: mt phn cha danh sch cc thng tin mu (file list), mt phn cha im sc k ca mu hin ang c chn (plot window), mu t danh sch tp tin, v th ba cha bo co hin th cc kt qu t nhng im mu (report window).

Mn hnh hin th ca bt k ca s c th c bt hoc tt bng cch s dng cc lnh n hoc nt thanh cng c. Mi ca s cng c th c ng li bng cch s dng Close trn ca s i km chnh n. Mi ca s c th c bt gia kch thc bnh thng v ti a theo cch thng thng cho cc ca s ni (tc l vo nt mi tn kt hp trong khung).S i km s c thc hin nh m ca s i km hon ton lp y ca s m. Trong khi n s c th m kho mt ca s c rt t im khi.Docking s c s dng cho php b tr linh hot vi kch thc tng i d dng ca cc ca s, thay v bt k mc ch no khc.Khi mt tp tin duy nht c chn, ca s hin th cc th sc k t ca s tp tin v bo co hin th kt qu (nu c sn). Sc c th c ch thch vi thng tin mu, tn nh v / hoc thng tin nh cao khc, ng nn v nhn thm vo bi ngi s dng.Cc d liu kt qu Tutorial Part 2 86, c th c hin th di dng mt bng peak n gin v / hoc trong mt bo co TotalChrom nh dng c th c xc nh bi tp tin .rpt.Khi c nhiu hn mt tp tin c chn trong danh sch tp tin, tt c sc s c hin th, nh dng xp chng hoc ph ln nhau v ca s bo co hin th mt bng tm tt cc kt qu t tt c cc tp tin. Khng c gii hn ty ca s lng sc c hin th - ch xp chng kh ph hp cho s lng th bt k.V d sau y ch ra quy trnh xem xt v ph duyt c s dng nh th no. V d ny c pht trin minh ha mt s kha cnh quan trng ca c im k thut Xem xt v ph duyt. N khng n gin cng khng phc tp.N m t qu trnh xem xt v ph duyt. N khng hon ton chnh xc.In this Example1. Michael ng nhp vo TotalChrom trn mt trong nhng my tnh trong phng th nghim v m Xem xt v ph duyt mi trng bng cch nhn vo biu tng trong TotalChrom Navigator.

a. Chn ba mu bo co TCR i din cho CK46123, CK46124 v b. CK46125 trong hp thoi Open File v nhn OK.2. Ct Signature Status trong danh sch tp tin ch ra nh gi ca c ba TCR, nh Michael hy vng (Vicky n vo ngy ch nht xem xt li mu sau khi James hon thnh chy sc k). sc v bo co cho cc mu u tin c hin th trong ca s Plot and Report. ng phng to sc kim tra s tch hp v c s cho mi nh.3. Tt c trng tha ng. Michael lp i lp li qu trnh ny cho hai tp tin TCR cn li. Cc sc k v tch hp c v tt p cho tt c ba mu sc k.4. By gi ng mun xem xt cc bo co cht ch hn trc khi k kt , v th ng chn lnh All Reports t Sign menu.Ca s Report Review xut hin vi bo co Actives cho mu CK46123 hin th. Michael lu rng cc Review, Final Review and Hold c kch hot trn thanh cng c Sign. Trong khi Approve and Final Approve c v hiu ha.

5. Anh ta s khng s dng Review t khi cng ty SOP ch yu cu hai mc xem xt. ng cng hy vng mnh s khng s dng Hold v vic t chi cc l sn phm dn n rt nhiu th tc giy t v s ch khng mong mun t qun l cp trn. Mt khi ng p dng mt Final Review xem xt cc bo co, chng s sn sng cho Susanna ph duyt cui cng.6. Michael Zoom xem bo co d dng. Sau , ng kim tra cc thng tin tiu cn thn m bo ng phng php c s dng. Cun bng kim tra bng peak m bo s lng bo co ca tng thnh phn hot ng v cc thng s ph hp nm trong gii hn. Khi ng tha mn, Michael nhp chut vo Final Review.7. Bo co cho tp cht CK46123 sau c hin th. Michael xem xt li cc thng tin tiu biu mt ln na v kim tra hm lng ca mi tp cht. Tt c nhng gi tr nm trong gii hn v do Michael chn Final Review mt ln na.8. Michael lp i lp li kim tra ca mnh trn cc bo co CK46124. C hai u t yu cu, do , ng chn Final Review. Bo co CK46125 cng t, v vy ng chn Final Review. Bo co cho tp cht CK46125 c hin th. Michael lu rng cc tp cht ring trong gii hn nhng tng s qu cao.9. ng chn Hold. L do cho Hold c hin th, theo SOP, Michael g li gii thch v l do ti sao anh khng ph duyt bo co.10. ng nhn OK v ghi ch rng tn bo co trn thanh trng thi ca ca s Review Reports c theo sau bi (Hold Unsigned). l bo co cui cng trong thit lp. Nhn Finish kt thc.11. Hp thoi bo co ng nhp c hin th. iu ny cho thy ba tp tin TotalChrom Report ng la chn ban u l nt cao nht trn thang iu khin. Mi nt cao nht c hai nt th cp di n, i din cc bo co ring l. Status l ct c nh du ch ra Reviewed Final and one Hold.C:\data\september\09\CK46123.tcr C:\data\september\09\actives_r2.rpt Reviewed Final C:\data\september\09\impurities_r3.rpt Reviewed Final C:\data\september\09\CK46124.tcr C:\data\september\09\actives_r2.rpt Reviewed Final C:\data\september\09\impurities_r3.rpt Reviewed Final C:\data\september\09\CK46124.tcr C:\data\september\09\actives_r2.rpt Reviewed Final C:\data\september\09\impurities_r3.rpt Hold12. Michael chn Sign v hp thoi Electronic Signature hin th. 13. Nhp user name v password. 14. Chn OK, mn hnh quay li Review v Approve. Danh sch tp tin vn cha tp tin TCR cho 3 mu CK46123, CK46124 and CK46125. Ct Status hin th Reviewed Final cho 5 bo co u tin v Hold cho bo co cui cng.Khi Susanna bc vo phng Lab. Debbie ni cc php phn tch phi c thc hin li trc ngy cui tun. Susanna ni : Debbie khng vui v mt mu khng t, CK46125 c hm lng tng tp cht rt cao. Ta khng th chuyn i, cn nhng ci khc th sao?. CK46123 tt nht, CK46124 th trung bnh. Michael ni chng ta nn vui v 2 mu kia ok bn c mun kim tra li khng, Susanna ng v ngi vo my vi tnh. 15. Susanna chn All Reports trong Sign, xem xt ln lt cc bo co trong ca s Review Reports. 5 bo co u tin c nh du bi Reviewed Final ci m Michael ng .16. Final Approve click vo Final Approve cho mi bo co. Sau c click th 5, bo co cui cng c hin th v Susanna ghi ch rng nt Hold trn thanh trng thi. 17. C y click vo nt Comment, ch c trn thanh cng c Sign c sn. Mi bo co khng c duyt u c 1 Comment i km. Hp thoi hin th ch ra l do Michael khng duyt bo co. Bn vit sai chnh t phenylethyllonamide, Michael, Susanna tru iu khng ging bn. C y click Finish ng. Xem li tn bo co trn Sign Reports. Chn Sign v nhp tn, password vo Electronic Signature.Khi c y ng hp thoi, danh sch file trn Review and Approve c cp nht, ch ra Signature Statuses ca 3 file sample. C:\data\september\09\CK46123.tcr Reviewed Final C:\data\september\09\CK46124.tcr Reviewed Final C:\data\september\09\CK46124.tcr HoldKhi James i vo phng lab, Michael ni Good Morning, James. James bo anh ta hy vong Susanna vi Michael v Debbie i michael vn phng ca anh y. C y bo Jame a michael ti mt con ng ng. Ok, ti s n v xo i tin xu cho c y, hy gi cho ti nu bn cn. Michael gi Susanna khi c y ra khi phng lab. l nhng g ngi ban hng PerkinElmer ni. Susanna tr li li nhng phn mm TotalChrom software rt d s dng v ti khng bao gi c.S hng dn ny vit cho ngi mi s dng, tm tt ngn gn cch s dng, ci t phn mm TotalChrom. Cn nhiu iu khc cha c ua ra trong hng dn ny. Tt nht nn c k TotalChrom Users Guide hiu nguyn l hot ng v ng dng ca n ny. Ngi dng nn training v TotalChrom hiu r hn v phn mm ny. bit thm thng tin, lin h 1-800-762-4000. TotalChrom Support Team sn sng gip bn khi gp kh khn. Nhng ngi dng cc nc khc, lin h vn phng PerkinElmer gn nht.

GVHD SVTHHunh Th Hng Hoa 74 Nhm 2