St engthening and E pansion of CP007 Strengthening and Expansion of Postgraduate Research Capabilities for the Development and Production of Inexpensive i k d l i Livestock and Poultry Vaccines Dr. Md. Bahanur Rahman (SPM) Dr. Md. Tanvir Rahman (ASPM) Bangladesh Agricultural University
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St engthening and E pansion of
CP007
Strengthening and Expansion of Postgraduate Research Capabilities for the
Development and Production of Inexpensive i k d l iLivestock and Poultry Vaccines
Dr. Md. Bahanur Rahman (SPM)Dr. Md. Tanvir Rahman (ASPM)
Bangladesh Agricultural University
Department of Microbiology and Hygiene: lAt a Glance
• Established: 1961• Established: 1961
• Degree offered: PhDPhDMS in Microbiology MS in Veterinary Public Health & Food Hygieney yg
• Courses offered at undergraduate level: Faculty of Vet. Science: 12 courses (22 Cr)Faculty of Anim. Husbandry: 4 courses (6 Cr)F lt f A il E & T h 2 (3 C )Faculty of Agril. Eng. & Tech.: 2 courses (3 Cr)
Departmental Teachers’ StrengthDepartmental Teachers’ Strengthp gp g
Procurement of state-of–art equipment andinstruments to enhance scientific capabilities ofthe relevant laboratories
E h t d i t f f i l Enhancement and improvement of professionalskills and research capabilities of academic staff,MS and PhD students
Improvement of IT facilities for PG students
Evaluation of quality safety potency and efficacy Evaluation of quality, safety, potency and efficacyof developed vaccines
Public-private partnership (PPP) establishment Public private partnership (PPP) establishment
Overall progress of the projectMajor Activities Present SituationRenovation and modernization of 6 laboratories, Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) and the Dept.Enrolment of MS and PhD students
Completed (6 Labs, 4 labs in LPVRPC, corridor, walls, ceiling, Enrolment of MS and PhD students
Local training floors)
Procurement and Installation of equipment / instruments and application in research
Completed (19 equipment & pp q pinstruments ) and research is in progress
IT facilities of the PG students Internet and bioinformatics facilities with PC for PG students
Vaccine Seeds, seed repository, inexpensive i d l t
Primary trial of 4 i d t d dvaccines development
Establishment of Reference Laboratory
vaccines conducted and genomic analysis in progressMolecular Microbiology y gy& Tissue culture Ref Lab established
Vaccines’ quality, safety, potency and efficacy
Molecular Microbiological Research F iliti D l dFacilities Developed
• Molecular characterization of bacteria and virus targeting their DNA and RNA using PCR and RT-PCR approach, cloning and sequencing etcE t bli h t f M d Ti C lt f iliti• Establishment of Modern Tissue Culture facilities for isolation, identification and characterization of animal and poultry viruses
• Installation of Cell imaging and documentation systemDNA d RNA i li ti d d t ti• DNA and RNA visualization and documentation system
• Protein analysis system e.g., SDS-PAGE andProtein analysis system e.g., SDS PAGE and Westerm blotting
Renovation of the Department and Departmental laboratories
Major Renovation WorksMolecular Bacteriology labMolecular Virology labo ecu a o ogy abImmunology LabVaccinology LabVaccinology LabTissue culture Lab Food microbiology LabFood microbiology Lab
4 Labs of LPVRPC including corridorsRenovation of DepartmentalRenovation of Departmental Corridors, Floor, Walls, Ceiling, glass doors etc
Laboratories Renovation
Lab before HEQEP Project Lab after HEQEP Project
Laboratories RenovationLaboratories Renovation
Lab before HEQEP ProjectLab before HEQEP Project
Lab after HEQEP Project
Laboratories Renovation
Lab before HEQEP Project Lab after HEQEP Project
Laboratories Renovation
Lab after HEQEP ProjectLab before HEQEP Project
Laboratories RenovationLaboratories Renovation
Lab after HEQEP Project
Departmental Renovation
Corridor before HEQEP Project Corridor after HEQEP Project
Renovation of Livestock and Poultry Vaccine
Research and ProductionResearch and Production Centre (LPVRPC)
LPVRPC R t dLPVRPC Renovated
LPVRPC after HEQEP Project
ll i f fInstallation of state-of-art Equipment forEquipment for
Postgraduate Researchg
Equipment Installed80 C d f Refrigerated Micro-centrifuge-80 C deep freezer Refrigerated Micro centrifuge
machine
Shaker incubatorAutoclave
Equipment InstalledI t d i W t b th h k i b tInverted microscope Water bath shaker incubator
IT and informatics facilities PhotocopierIT and informatics facilities Photocopier
Sero-typing of FMDVSero typing of FMDVFMDV serotypes -O, A and Asia-1 have been isolated, adapted in BHK-21 cell and finally identified by sandwich ELISA and RT-PCR for Master Seedand finally identified by sandwich ELISA and RT PCR for Master Seed development
FMDV sero-types O, A and Aisa-1 seedsFMDV sero types O, A and Aisa 1 seeds
Field trial of Trivalent FMD vaccinee d t a o a e t acc e
Scientific BreakthroughScientific Breakthrough FMDV serotypes-O, A and Asia-1 isolated, yp
identified, adapted in BHK-21 cell NDV adapted in BHK-21 cell
Experimental BEI inactivated trivalent FMD killed ND FC and HS vaccinesFMD, killed ND, FC and HS vaccines vaccine developed
Primary trial of these vaccines completed & Primary trial of these vaccines completed & second trial in progress
G i l i i Genomic analysis in progress
Mi bi l R itMicrobial Repository DevelopmentDevelopment
Microbial RepositoryMicrobial Repository
D l t d ti f•Development and preservation of bacterial and viral vaccine seeds
•Establishment of bacterial and viral strain repository from filed isolates
Microbial Repository
1901 bps
574 bps
Amplification of Hexon Amplification of 16s RNA gene ofAmplification of Hexon gene of EDS-76 virus
Amplification of 16s RNA gene of multi-drug resistant E. coli from chicken
Microbial Repository
Pseudomonas aeruginosaStaphylococcus aureus
500 bp 540 bp
Bacterial RepositoryBacterial Repository
Lab VisitsLab Visits
Laboratory Visit
Laboratory Visit
Laboratory Visity
Field VisitField Visit
Field Visit and vaccination campaignField Visit and vaccination campaign
Field Visit and vaccination campaign
Local and International Collaboration
Local CollaborationLocal Collaboration
FAO, Bangladesh
Department of Livestock Services (DLS)
Phenix Hatcher Ltd, Gazipur
International Collaboration EstablishedEstablished
Dr Roman Dziarski Dept of Microbiol & ImmunolDr. Roman Dziarski, Dept. of Microbiol. & Immunol., Indiana Univ., School of Med. USA
Dr Z Kornelius Associate Professor Dept of ProteinDr Z. Kornelius, Associate Professor, Dept. of Protein Evolution, Max Planck Institute, Germany
Prof Shi Tung Liu Mol Microbial Genetics Lab Dept ofProf. Shi-Tung Liu, Mol. Microbial Genetics Lab., Dept. of Microbiol & Immunol., Chang-Gung Univ., Taiwan
Dr Monica Cagiola Istituto Zooprofilattico SperimentaleDr. Monica Cagiola, Istituto Zooprofilattico Sperimentale dell'umbria e delle marche via g. salvemini, 06100, Perugia, Italy
Impact on Higher Studies and Research
Impact on Higher Studies and Research• Higher education quality and innovative research capability
developed
• Modern laboratories established and in use• Modern laboratories established and in use
• State-of art equipment procured , installed and in use
• Ultrapure grade chemicals & reagents procured and using for research
• Molecular microbiology, Tissue culture , vaccinology andMolecular microbiology, Tissue culture , vaccinology and immunology research facilities developed
• International & national collaboration established
• Establishment of PPP for production and distribution of vaccines in progress
T h & PG d f h D h F l ll h• Teachers & PG students of the Dept., the Faculty as well as the University benefited from implementation of this project
Acknowledgment• World Bank• Ministry of Education• Ministry of Education• UGC• HEQEP• HEQEP• BAU • Faculty of Veterinary Science BAU• Faculty of Veterinary Science, BAU• Dept. of Microbiology & Hygiene, BAU• FAO Bangladesh• FAO, Bangladesh• Department of Livestock Services (DLS)
Thank you all from the Department of Microbiology and Hygiene, BAU