Bacteriophage Antibiotic resistant bacteria eXolution

6F, Sambo building ,296, Jungdae-ro, Songpa-gu, Seoul, Republic of Korea, Postal code 05724

Powder

Bacteriophage

Package5 kg / 10 kg pack

SpeciesSwine, Cattle, Poultry

Active ingredient

Dosage

Bacillus subtilisBacteriophage targeting

Salmonella spp.Escherichia coli

Clostridium perfringensStaphylococcus aureus

General dosage : Mix 300~500 g / MT of feedPiglet dosage : Mix 1 kg / MT of feed

IndicationControlling pathogens susceptible to eXolution bacteriophage, Growth promoter

ApplicationSpecies : Poultry, Swine, Cattle | Type : Feed additive powder type

StorageStore in a cool dry place. Avoid direct sunlight

Shelf life1 year after manufacture date

eXolutionBacteriophage

Effective to Biofilm

Other advantages of Bacteriophage

Bacteriophage can destroy and penetrate the biofilm, so that not only bacteriophage

but also antibiotics can reach to bacteriainside biofilm.

Bacteriophage can control antibioticresistant bacteria.

Because of difference in mode ofaction between bacteriophage and antibiotics,

chance of resistance is very lowwith bacteriophage.

Biofim

AntibioticAntibiotic

Bacteria

“PAS effect”Phage-AntibioticsSynergistic effect

Bacteriophage attacks bacteria with specific receptor only.Therefore beneficial bacteria such as Lactobacillus are safe from eXolution andit will help health of livestock.

- No side effect according to dosage

- No withdrawal time

Effective toAntibiotic resistant bacteria

* PAS : Phage Antibiotic Synergy

Bacteriophage

eXolutionWhat is Bacteriophage?

How Bacteriophage kills Bacteria?

Challenge trial

·Natural material - Most abundant in nature, 1031 in earth, can be found in everywhere

·Safe to human and animal – Attack only target bacteria

·Have bacteria as a host, much smaller than bacteria

·Found prior to antibiotic discovery (1896)

Layer Egg productioneXolution

SwineeXolution

BroilereXolution

Harmful Microflorachange in feces

eXolution

Harmful Microflorachange in organs

eXolution

- Egg production, % -

0 to 3 WK 4 to 6 WK

90.892

89.9

92.1

Con eXolution

- Bacteria Population, Log10 cfu/g -

- Bacteria population, Log10 DNA copy numbers/g -

E. coli Salmonella

6.756.28

3.472.11

Con eXolution

1.566

1.5411.528

FCR

1.62

1.55 1.56

FCR

Con Avilamycin eXolution

5.665.27

4.89

Salmonella

Con 0.04% eXolution 0.08% eXolution

Con BMD eXolution

Items Con Apramycin eXolution

G/F

Dry matter, %

Nitrogen, %

Energy, %

0.357

77.40

77.00

76.63

0.377

80.08 *

78.43

79.22

0.371

79.57 *

79.22 *

78.46

Items Con eXolution

ADG, g

ADFI, g

FCR

737

2,079

2.82

764

2,129

2.79

Log10 cfu/g Con Apramycin eXolution

E.Coli

Salmonella

6.55

3.62

6.00 *

2.57 *

6.14 *

2.02*

Fecal shedding Log10 cfu/g Con Sal. Enteriridis SE+0.2% eXolution

No. at weaningPiglets / litter, kg

BW at weaningPiglets / litter, kg

ADG, g / pig

Sow feed without eXolution

eXolution eXolution- -Creep feed

10.1

56.8

234

10.4

65.1

260

10.2

57.6

235

10.6

65.3

258

eXolution

SE+0.1% eXolution

3 dpi 0 3.45

1.58

3.17

1.58

2.80

0.7106 dpi

Phage Attaches to receptor site onbacterial cell wall,

penetrates it, and insert its DNA.

The phage DNA directsthe cell’s metabolism

to produce viral components-proteinsand copies of phage DNA.

Empty phage headsare synthesized.

Heads are packedwith DNA.

Collars, sheaths, and base plateshave been attached to heads.

Tail fibers are added last.

Bacterial cell lyses,releasing completed

infective phages.

* FCR : Feed Conversion Ratio

Nucleus : 2,800nmNucleus : 2,800nm

E.coli : 2,000nmE.coli : 2,000nm

Phage : 95nmPhage : 95nm

Eukaryotic cell : 10,000nmEukaryotic cell : 10,000nm