Bacteria Isolation and Characterization Presentation

ISOLATING BACTERIA FROM TROPICAL CONTAMINATED

SOILS IN PUERTO RICO

RISE SPRING 2015

Sara Diaz

Patricia Rodriguez

Camille Ruiz

Justin Cotto

SAMPLING LOCATIONS

Sample Coordinate

s

Environmental

description

Locatio

n

Date/

Time

Ruiz C

- #1

18°6’55.1”N;

66°9’36.2”W

Moist (underneath

grass),

5 cm; 72°F

Cayey,

PR

January/

28/15

7:30 p.m.

Cotto J

- #2

18.15º N

66.18º W

Moist environment

(above grass), 76ºF

Cidra,

PR

January/

29/15

8:00 a.m.

SAMPLING LOCATIONS

Sample Coordinates Environmental

description

Location Date/

Time

Diaz S

-#1

18°15'15.7"N 66°06'06.9"W

Moist environment

80°F

Aguas

Buenas,

P.R.

January/

27/15

4:26 p.m.

Rodríguez P

-#2

18°15'15.7"N 66°06'06.9"W

Moist environment

80°F

Aguas

Buenas,

P.R.

January/

27/15

4:26 p.m.

RESULTS FROM DIFFERENT PROCEDURES

Process CLRV30TSA01A CLRV30M01B JCR30TSA01A

Gram Stain Negative Positive Positive

Bacterial Morphology Coccus Bacillus Bacillus

PCR/Electrophoresis

#1

Negative Negative Negative

PCR/Electrophoresis

#2

Positive Negative -

Antibiotic Production Negative Negative Negative

GRAM STAIN RESULTS

CLRV30TSA01A CLRV30M01B JCR30TSA01A

POSITIVE ELECTROPHORESIS

RESULTS FROM DIFFERENT PROCEDURES

Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01

Gram Stain Positive Positive

Bacterial Morphology Bacillus Bacillus

PCR/Electrophoresis

#1

Negative Negative

PCR/Electrophoresis

#2

Negative Negative

Antibiotic Production Negative Negative

GRAM STAIN RESULTS

S15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B

ANTIBIOTIC RESISTANCE TEST RESULTS

Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A

Kanamycin 2.0 cm 1.0 cm 2.0 cm

Penicillin 0.0 cm 0.0 cm -

Tetracycline 1.6 cm 2.4 cm 2.2 cm

Gentamicin 1.9 cm 0.9 cm -

Chloramphenicol 0.0 cm 0.0 cm 2.5 cm

Bacitracin 0.0 cm 0.0 cm 1.5 cm

Vancomycin 0.0 cm 0.0 cm -

Streptomycin 1.6 cm 1.0 cm 1.9 cm

ANTIBIOTIC RESISTANCE TEST RESULTS

Antibiotic S15UPRC-

RISESCDM30T01B

Streptomycin 2.2 cm

Chloramphenicol 2.3 cm

Erythromycin 1.0 cm

Kanamycin 1.6 cm

Gentamicin 0.6 cm

AntibioticS15UPRC-RISEPR30M01

Rifampin 0.13 cm

Kanamycin 0.16 cm

Vancomycin 0.17cm

Streptomycin 0.30 cm

Tetracyclin 0.14 cm