BacMagic ™ Systems plEx/Bac ™ Expression Vectors Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
The original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
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EMD Chemicals Inc.480 South Democrat Road Gibbstown, NJ 08027
BacMagic™ SystemsplEx/Bac™ Expression Vectors
Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
For more information or to orderNovagen products, contact EMD Chemicals.
You can also order Novagen products throughour distribution partner, VWR InternationalPhone 800 932 5000Web www.vwr.com
LT 10003558 10/09
BacMagic™, pIEx/Bac™, pBAC™ and TriEx™, GeneJuice®, InsectDirect®, BacVector®, GST•Tag™ and Novagen® are trademarks of EMD Chemicals Inc. or its affiliated companies.
Strep•Tag® is a registered trademark of Institut fuer Bioanalytik GmbH.
Product prices and availability are subject to change. Products are warranted only to conform to the specifications set forth on their label/packaging and/or certificate of analysis at the time of shipment or for the expressly stated duration. NO OTHER WARRANTY WHETHER EXPRESS, IMPLIED OR BY OPERATION OF LAW IS GRANTED. The products are intended for research purposes only and are not to be used for drug or diagnostic purposes, or for human use. The products may not be resold or used to manufacture commercial products without the prior written approval of EMD Chemicals. EMD makes no warranties of any kind for any technical advice provided by EMD, or for any results occurring as a result of the application of such advice. Product users must review the full instruction set included with the product prior to use. All sales are subject to EMD Chemical's complete Terms and Conditions of Sale.
BacMagic™ SystemThe original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.
BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
pIEx/Bac™ Expression VectorspIEx/Bac™ dual purpose vectors are the perfect combination with BacMagic™ System for baculovirus infection and expression. Because the pIEx/Bac™ vectors are compatible with both plasmid-mediated and baculovirus expression, it is easy to use a single construct in both expression systems for HT screening and optimized high-yield protein expression.
Homologous recombination schemes of the BacMagic™-3 System
∆chiA
Gene ORF1629
Recombinant virus
BacMagic™-3 DNA
∆chiA
lef2 Bac
Gene ORF1629
lef2
lef2
1629
Transfer plasmid
∆v-cath
∆v-cath
∆p10
∆p10
Day 1 Day 5 Day 8 - 10 Days 11 - 13
Cotransfect insect cells with recombinant transfer plasmid
plus an AcNPV BacMagic™ DNA
Harvest recombinant baculovirus; screen for expression; amplify viral
• Faster baculovirus production without tedious plaque purification• Compatible with pIEx/Bac™, pBAC™, pTriEx™, and other transfer plasmids using the lef2/603 and ORF1629 recombination sites• Deletion of chitinase to maximize secreted and membrane targeted protein production• Includes Insect GeneJuice® Transfection Reagent for high efficiency transfection
New pIEx/Bac™-4 and –5 vectors encode an N-terminal GST•Tag™ sequence (Table 1). The GST•Tag™ affinity tag is popular for insect cell expression because it may improve solubility for some proteins.
BacMagic™ SystemThe original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.
BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
pIEx/Bac™ Expression VectorspIEx/Bac™ dual purpose vectors are the perfect combination with BacMagic™ System for baculovirus infection and expression. Because the pIEx/Bac™ vectors are compatible with both plasmid-mediated and baculovirus expression, it is easy to use a single construct in both expression systems for HT screening and optimized high-yield protein expression.
Homologous recombination schemes of the BacMagic™-3 System
∆chiA
Gene ORF1629
Recombinant virus
BacMagic™-3 DNA
∆chiA
lef2 Bac
Gene ORF1629
lef2
lef2
1629
Transfer plasmid
∆v-cath
∆v-cath
∆p10
∆p10
Day 1 Day 5 Day 8 - 10 Days 11 - 13
Cotransfect insect cells with recombinant transfer plasmid
plus an AcNPV BacMagic™ DNA
Harvest recombinant baculovirus; screen for expression; amplify viral
• Faster baculovirus production without tedious plaque purification• Compatible with pIEx/Bac™, pBAC™, pTriEx™, and other transfer plasmids using the lef2/603 and ORF1629 recombination sites• Deletion of chitinase to maximize secreted and membrane targeted protein production• Includes Insect GeneJuice® Transfection Reagent for high efficiency transfection
New pIEx/Bac™-4 and –5 vectors encode an N-terminal GST•Tag™ sequence (Table 1). The GST•Tag™ affinity tag is popular for insect cell expression because it may improve solubility for some proteins.
EMD Chemicals Inc.480 South Democrat Road Gibbstown, NJ 08027
BacMagic™ SystemsplEx/Bac™ Expression Vectors
Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
For more information or to orderNovagen products, contact EMD Chemicals.
You can also order Novagen products throughour distribution partner, VWR InternationalPhone 800 932 5000Web www.vwr.com
LT 10003558 10/09
BacMagic™, pIEx/Bac™, pBAC™ and TriEx™, GeneJuice®, InsectDirect®, BacVector®, GST•Tag™ and Novagen® are trademarks of EMD Chemicals Inc. or its affiliated companies.
Strep•Tag® is a registered trademark of Institut fuer Bioanalytik GmbH.
Product prices and availability are subject to change. Products are warranted only to conform to the specifications set forth on their label/packaging and/or certificate of analysis at the time of shipment or for the expressly stated duration. NO OTHER WARRANTY WHETHER EXPRESS, IMPLIED OR BY OPERATION OF LAW IS GRANTED. The products are intended for research purposes only and are not to be used for drug or diagnostic purposes, or for human use. The products may not be resold or used to manufacture commercial products without the prior written approval of EMD Chemicals. EMD makes no warranties of any kind for any technical advice provided by EMD, or for any results occurring as a result of the application of such advice. Product users must review the full instruction set included with the product prior to use. All sales are subject to EMD Chemical's complete Terms and Conditions of Sale.