Microgen Bioproducts Ltd Microgen Bioproducts Ltd Microgen Bioproducts Ltd Microgen Bioproducts Ltd Bacillus ID MID Bacillus ID MID Bacillus ID MID Bacillus ID MID- - -66 66 66 66 An identification system for An identification system for An identification system for An identification system for Mesophilic Mesophilic Mesophilic Mesophilic Bacillus species and Bacillus species and Bacillus species and Bacillus species and related genera associated with related genera associated with related genera associated with related genera associated with food poisoning and food spoilage food poisoning and food spoilage food poisoning and food spoilage food poisoning and food spoilage
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Bacillus ID MIDBacillus ID MID----66666666 An ... TrainingMID66 24.02.09… · Bacillus ID MIDBacillus ID MID----66666666 Biochemical identification system that consists of 24 substrates
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Bacillus ID MIDBacillus ID MIDBacillus ID MIDBacillus ID MID----66666666
An identification system for An identification system for An identification system for An identification system for MesophilicMesophilicMesophilicMesophilic Bacillus species and Bacillus species and Bacillus species and Bacillus species and related genera associated with related genera associated with related genera associated with related genera associated with food poisoning and food spoilagefood poisoning and food spoilagefood poisoning and food spoilagefood poisoning and food spoilage
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Bacillus ID MIDBacillus ID MIDBacillus ID MIDBacillus ID MID----66666666
�General information
�Procedure
�Competitors
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Bacillus ID MIDBacillus ID MIDBacillus ID MIDBacillus ID MID----66666666
�Biochemical identification system that consists of 24 substrates
�Identification of mesophilic Bacillus and related Genera
�Substrates based on conventional methods: direct correlation with standard methods
�The last well of the second strip is a control which facilitates differentiation between positive and negative reactions
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List of organisms List of organisms List of organisms List of organisms in databasein databasein databasein database
�The only system developed for the identification of mesophilic
Bacillus and related genera of food poisoning & food spoilage
importance.
�Presence of control well acts as a aid in identification
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Competitors Competitors Competitors Competitors
�Biomerieux (API 20E + API50CHB )
�BD (Crystal Gram positive)
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bioMerieuxbioMerieuxbioMerieuxbioMerieux, API, API, API, API
�System that consists of 70 substrates
�Not all substrates are relevant to result: extra cost
and wastage
�Adaptation of two separate systems (API 20E &
50CHB), not a system devoted to Bacillus
�Requires the purchase of two separate systems
�Taxonomy employed is out-of-date
�Expensive per test
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BD,Crystal Gram Positive kit
� System is incubated for 18 hours and consists of 30 substrates for the identification of 121 Gram-positive organisms
� Not a dedicated system for the identification of Bacillus
� Self-inoculating device which remains closed after inoculation
�Wells are 2mm in diameter and very difficult to read
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Microgen Bacillus-ID vs
API50CHB+API20E
� A total of 49 strains of “Bacillus and related genera” comprising strains from recognised culture collections and isolates from various food and beverage sources were identified using the Microgen®Bacillus ID (MID - 66) and the API System comprising the API 50CHB and the API 20E
Total
Tested
MID-66 API
B. firmius 2 2 2
B. cereus 11 11 11
B. licheniformis 11 11 11
P. macerans 1 1 1
B. megaterium 1 1 1
B. pumilus 4 4 4
B. sphaericus 3 3 3
B. subtilis(1) 11 11 11
B. thuringiensis(2) 2 2 0
P. alvei 1 1 1
B. circulans 2 2 2
Total 49 49 47
Percent Correct 100.0% 95.9%
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Microgen Bacillus-ID vsAPI50CHB+API20E
� The Microgen® Bacillus ID successfully identified all of the isolates tested. The API system combination of the 50CHB and 20E was also able to identify 100% of the isolates however some taxonomyemployed was found to be out of date (see below):
2 isolates identified a B.amyloliquefaciens - a member of the B.subtilis
group
2 B.thuringiensis not included in API database - isolates identified a B.cereus. B.thuringiensis is a member of the B.cereus group. If the ID