AwalMart_Catalogue2

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Researches by scientists throughout the world have proved that these herbs can be used for the diseases indicated.

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1) 5-Elements:

Traditionally used for symptomatic relief of problems related to:

1). Lethargy 2). Poor immunity 3). Sleep problem (Insomnia) Herbs:

Polygonum Multiflorum Semen Sinalpis Alba Poria Cocos Wolf Fructus Schisandra Chinensis Radix Salvia Miltiorrhiza Overall TCM Pathology

Promotes healthy kidney, liver, spleen, heart and lungs for the purposes of Restorative Immunology. Supports the cardiovascular system, promotes healthy blood pressure and blood flow as well as healthy cholesterol level. Promotes healthy bones and women’s vitality. Promotes uninterrupted sleep (for those who suffer from insomnia). Prevents excessive perspiration. Increases red blood cell production. For breathlessness. 1. Radix Polygonum Multiflorum

Function of the herb:

• To tone up liver and kidney and replenish their vital essence • Treat fatty liver, increase red blood cells and reduce blood fats • Exert same effect as adrenoticortical hormone • Nourish blood • For aching back and knee • Treat tiredness and exhaustion • Protect heart from damage from free radicals • Anti aging

• Improve immune system through its antioxidant effect • Treat cholesterol problem

• Retard and treat arteriosclerosis (hardening of blood vessels) • Protect liver • Regulate blood

• Increase red blood cell production

Polygonum Multiflorum: Research shows that "The extract of the root of Polygonum multiflorum exhibited a significant antioxidant activity assessed by the DPPH radical scavenging activity in vitro. The bioassay-guided fractionation of the extract yielded a stilbene glucoside, (E)-2,3,5,4'-tetrahydroxystilbene-2-O-beta-d-glucopyranoside (1) as an active constituent responsible for the antioxidant property. Compound 1 demonstrated a moderate DPPH radical scavenging activity (IC50, 40 microM), while the corresponding deglucosylated stilbene 2 exhibited a much higher activity" (IC50, 0.38 microM). “The radical scavenging effects of stilbene glucosides from Polygonum

multiflorum” Arch Pharm Res 2002 Oct;25(5):636-9.

2. Perilla Frutescens


• Used as anti-asthmatic herb • Expectorant for chronic bronchitis

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Chronic systemic inflammation can inflict degenerative effects throughout the body. A primary cause of this destructive cascade is the production of cell-signaling chemicals known as inflammatory cytokines. Along with these dangerous cytokines, imbalances of hormone-like messengers called prostaglandins also contribute to chronic inflammatory processes. The body needs fatty acids to survive and is able to make all but two of them: linoleic acid, in the omega-6 family and linolenic acid, in the omega-3 family. These two fatty acids must be supplied by the diet and are therefore considered essential fatty acids (EFAs). Perilla is rich in linolenic/alpha-linolenic acid (ALA). Since alpha-linolenic acid or ALA cannot be manufactured by the body it must be obtained from food. ALA, as well as the fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), belongs to a group of fatty acids called omega-3 fatty acids. EPA and DHA are found primarily in fish while ALA is highly concentrated in plants like perilla. Once ingested, the body converts ALA to EPA and DHA, the two types of omega-3 fatty acids more readily used by the body. If we can consume sufficient ALA then intake of EPA and DHA is optional. Omega-3 fatty acids help reduce inflammation and most omega-6 fatty acids tend to promote inflammation. Omega-3 fatty acids have been shown to reduce inflammation and help prevent certain chronic diseases such as lung disease like asthma and arthritis. These essential fatty acids are highly concentrated in the brain and appear to be particularly important for cognitive "The leaves of Perilla frutescens Britton (Labiatae) are one of the most popular garnishes in Japan, used as an antidote for fish and crab meat allergy or as a food colorant. The present study was conducted to evaluate its anti-allergic effect and to identify its active constituents using mice ear-passive cutaneous anaphylaxis (PCA)-reaction. 48 hr after the cutaneous injection of anti-ovalbumin serum into the ears of mice, ovalbumin and evansblue dye were intravenously injected. Perilla was extracted with boiling water, and intraperitoneally injected 15 min before ovalbumin-treatment. Thirty min after ovalbumin-treatment, the ears were removed and the colorant in the ear was colorimetrically quantitated. Perilla extract significantly suppressed the PCA-reaction, which was brought about by rosmarinic acid with a partial contribution from some macromolecular compounds. The anti-allergic titer of rosmarinic acid was more effective than tranilast, which is a modern anti-allergic drug. Perilla and rosmarinic acid are potentially promising agents for the treatment of allergic diseases." "Anti-

allergic effect of Perilla frutescens and its active constituents." Phytother Res. 2003

Mar;17(3):240-3

Okamato M, et al.Dept of Medicine, Misasa Medical Branch, Tottori, Japan. Dietary sources of alpha-linolenic acid, such as perilla seed oil, may have the capacity to inhibit the generation of leukotrienes by leucocytes in patients with asthma, as has been reported with the consumption of other long-chain n-3 fatty acids. The factors affecting the suppression of leukotriene (LT) C4 generation by leucocytes were examined by comparing the clinical features of patients with asthma who had been given dietary perilla seed oil (n-3 fatty acids). Group A consisted of patients in whom the leucocytes generation of LTC4 was suppressed by dietary perilla seed oil. Group B consisted of those in whom LTC4 generation was not suppressed. Conclusions: Dietary supplementation with perilla seed oil in selected patients with asthma suppresses the generation of LTC4 and is associated with clinical features such as respiratory function and lipometabolism. “Effects of perilla seed oil supplementation on

leukotriene generation by leucocytes in patients with asthma associated with lipometabolism.”

Int Arch Allergy Immunol 2000 Jun; 122 (2) 137-42.

Okamoto M. et al., Okayama University Medical School, Tohaku-gun, Tottori. The effects of perilla seed oil (n-3fatty acids) on bronchial asthma were compared with the effects of corn-oil (n-6 fatty acids) in relation to the pulmonary function and the generation of Leukotriene b4 (LTB4) and C4 (LTC4) by leucocytes. 14 asthmatics subjects were divided randomly into two groups: one group of 7 consumed perilla seed oil-rich supplementation and the other group of 7 consumed corn-oil rich supplementation for 4 weeks. Conclusion: The results suggest that perilla seed-oil-rich supplementation is useful for the treatment of asthma in terms of suppression of LTB4 and LTC4 generation by leucocytes, and improvement of pulmonary function. “Effects of dietary

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supplementation with n-3 fatty acids compared with n-6 fatty acids on bronchial asthma.”

Intern Med 2000 Feb; 39(2):107-11.

3. Semen Sinapis Alba


• Used for cough • For stuffy feeling in the chest

Research: "Sinapis alba is effective for strengthening the lungs. The effect of naturally occurring allyl and phenyl isothiocyanates found in this herb in the inhibition of B16F-10 melanoma cells-induced metastasis has been studied in C57BL/6 mice. The two compounds were administered by three different modalities. Simultaneous administration of the compounds with tumour induction was found to produce significant reduction in the lung tumour nodule formation; of the two compounds studied, allyl isothiocyanate have shown to be more effective and it produced 93% inhibition of tumour nodules while phenyl isothiocyanate has an inhibition of 77.3%. Prophylactic administration also produces considerable effect but no effect was observed by their administration after the tumour development. Lung collagen hydroxyproline, lung uronic acid, lung hexosamine content, serum sialic acid and serum GGT levels were also estimated and the enhanced levels of these parameters in the control animals were found to be reduced by the simultaneous administration of these isothiocyanates; these data were also correlated with the inhibition of lung tumour nodules produced and the increase in life span of the animals." "Effect of naturally occurring allyl and phenyl isothiocyanates in the

inhibition of experimental pulmonary metastasis induced by B16F-10 melanoma cells.

Fitoterapia 2003 Jun;74(4):355-63

"A calcium-soluble protein isolate (CSPI) was prepared from the supernatant obtained after addition of 0.75 M calcium chloride to a pH 5.0 aqueous extract of yellow mustard (Sinapis alba) seed meal. Total amino acid analysis showed that the CSPI has significantly higher (p < 0.05) contents of glutamic acid + glutamine, cysteine, and proline when compared to the precipitated, calcium-insoluble proteins. Peptide mass fingerprinting of tryptic peptides of the major polypeptides by mass spectrometry indicated that the CSPI is composed mainly of cruciferin proteins with a contribution from napins (the major allergenic proteins of S. alba). The S. alba CSPI had significantly higher (p < 0.05) protein solubility and emulsion formation ability in the presence of 0.75 M calcium chloride when compared to similar isolates prepared from Brassica juncea (brown mustard) and soybean seed meals. We suggest that the S. alba CSPI could be used to prepare calcium-fortified high protein liquid products." "Characterization of a calcium-soluble protein fraction from yellow mustard (Sinapis

alba) seed meal with potential application." J. Agric Food Chem. 2004 Sep 22;52(19):6030-4

4. Poria Cocos Wolf


• It has regulatory effect on human immune cells.

"The structure of a new triterpene derivative isolated from Poria cocos was determined to be 3 beta-p-hydroxybenzoyldehydrotumulosic acid by spectral and chemical methods. 3 beta-p-hydroxybenzoyldehydrotumulosic acid showed marked inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)- and arachidonic acid (AA)-induced ear inflammation in mice. The 50% inhibitory doses of 3 beta-p-hydroxybenzoyldehydrotumulosic acid were 0.27 and 1.25 mg per ear on TPA- and AA-induced inflammation, respectively." "3 beta-p-

hydroxybenzoyldehydrotumulosic acid from Poria cocos, and its anti-inflammatory effect."

Phytochemistry. 1998 Aug;48(8):1357-60

"Pachymic acid, 3-O-acetyl-16 alpha-hydroxytrametenolic acid, and poricoic acid B had been isolated from the sclerotium of Poria cocos Wolf. These compounds showed a strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice. At 0.2 mumol/mouse,

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these compounds markedly inhibited the promoting effect of 12-O-tetradecanoylphorbol-13-acetate (1 microgram/mouse) on skin tumor formation following initiation with 7,12-dimethylbenz[a]anthracene (50 micrograms/mouse)." "Inhibitory effects of lanostane-type triterpene acids, the components

of Poria cocos, on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage

carcinogenesis in mouse skin." Oncology. 1996 Sep-Oct;53(5):382-5.

"Triterpene carboxylic acids were isolated from the methanol extract of Hoelen, Poria cocos, and found to inhibit 12-O-tetradecanoylphorbol 13-acetate (TAP)-induced mouse ear edema. Their chemical structures were identified as 3 beta,-16 alpha-dihydroxylanosta-7,9(11),24-trien-21-oic acid, 16 alpha-hydroxydehydropachymic acid, 16 alpha-hydroxytrametenolic acid and dehydrotumulosic acid." "Isolation of inhibitors of TPA-induced mouse ear edema from Hoelen, Poria cocos."

Chem Pharm Bull (Tokyo) . 1996 Apr;44(4):847-9

"The antioxidant properties of twenty medical herbs used in the traditional Mediterranean and Chinese medicine were studied. Extracts from Forsythia suspensa, Helichrysum italicum, Scrophularia auriculata, Inula viscosa, Coptis chinensis, Poria cocos and Scutellaria baicalensis had previously shown anti-inflammatory activity in different experimental models. Using free radical-generating systems H. italicum. I. viscosa and F. suspensa protected against enzymatic and non-enzymatic lipid peroxidation in model membranes and also showed scavenging property on the superoxide radical. All extracts were assayed at a concentration of 100 microg/ml. Most of the extracts were weak scavengers of the hydroxyl radical and C. chinensis and P. cocos exhibited the highest scavenging activity. Although S. baicalensis inhibited the lipid peroxidation in rat liver microsomes and red blood cells, the extract showed inhibitory actions on aminopyrine N-demethylase and xanthine oxidase activities as well as an pro-oxidant effect observed in the Fe3+-EDTA-H2O2 system. The results of the present work suggest that the anti-inflammatory activities of the same extracts could be explained, at least in part, by their antioxidant properties." " Antioxidant activity of

anti-inflammatory plant extra." Life Sci. 2002 Jan 18;70(9):1023-33

"Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3 -hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells." "Inhibitory

effects of triterpenes isolated from Hoelen on free radical-induced lysis of red blood cells"

Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and

Pharmaceutical University.

5. Fructus Schisandra Chinensis


• Effective heart tonic • Used to treat liver and gall bladder stones • Used to treat insomnia and forgetfulness • Used to treat for neurasthenia (tiredness and exhaustion) • Researchers now recognize it is an “adaptogen” herb – capable of increasing the body’s

resistance to disease, stress and other debilitating process, boosts recovery after surgery and improves health of adrenals.

• It is used as an adjuvant herb to treat liver disease Schisandra Chinensis: “Schisandra chinensis (TURCZ.) BAILL., originally a Japanese-Manchurian endemite, yields a vegetable drug (Schisandrae fructus) with a number of very utilizable therapeutic effects. The paper reports the results of phytochemical and pharmacological-toxicological studies approximately from the year 1990 carried out both with the drug and, in particular, the principal

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isolated lignans of the dibenzo[a,c]cyclooctadiene type. The results confirm the validity of the historical use of the drug, in particular as a hepatoprotective, adaptogenic, and antioxidative agent. It is obvious that a very positive therapeutic effect based on the use of a complex mixture of its principal constituents because their biological effects are complementary and potentiate each other. At the same time, some lignans (e.g. gomisin A, gomisin N) are interesting as new prospective medicines” “Phytotherapeutic aspects of diseases of the circulatory system. 7. Schisandra chinensis (Turcz.)

Baill.): its composition and biological activity”. Ceska Slov Farm. 2001 Jul;50(4):173-80.

Schisandra Chinensis: "The effects of Schisandrin B (Sch B) and dimethyl-4,4'-dimethoxy-5,6,5',6'dimethylene-dioxy-biphenyl-2,2'-+ ++bicarboxylate (DDB) treatment on myocardial ischemia-reperfusion (IR) injury in isolated perfused rat hearts were examined under both in vitro and ex vivo conditions. In vitro administration of liposome-entrapped Sch B or DDB during reperfusion did not protect against myocardial IR injury, whereas ascorbic acid or Trolox supplemented perfusate produced protective effect, as evidenced by the significant decrease in the extent of lactate dehydrogenase leakage as well as an improvement in contractile force recovery. Myocardial protection afforded by N-acetyl-L-cysteine supplemented perfusate was not accompanied by the enhancement in contractile force recovery. In ex vivo experiment, pretreatment of Sch B (0.6/1.2 mmol/kg/day x 3) protected against IR-induced myocardial damage in a dose-dependent manner. The myocardial protection was associated with an enhancement in myocardial glutathione antioxidant status, as indicated by significant reductions in both the extent of IR-induced reduced glutathione depletion and inhibition of Se-glutathione peroxidase and glutathione reductase activities. In contrast, the inability of DDB pretreatment to enhance myocardial glutathione antioxidant status resulted in a failure in preventing IR injury. The ensemble of results suggests that the myocardial protection afforded by Sch B pretreatment, which was unlikely due to free radical scavenging action, may be mainly mediated by the enhancement of myocardial glutathione antioxidant status, particularly under oxidative stress conditions." “Schisandrin B protects against myocardial ischemia-reperfusion

injury by enhancing myocardial glutathione antioxidant status.” Mol Cell Biochem 1999

Jun;196(1-2):151-6.

6. Radix Salvia Miltiorrhiza

Function of the herb: • Promote blood circulation • Treat coronary heart disease • Increase red and white blood cells • Protect liver • Improve function of liver and spleen

In one study Salvia Miltiorrhiza (Sm) administration has been shown to reduce hepatic fibrosis (improve liver function) in rats. We investigated the hemodynamic effects of Sm on bile duct ligated (BDL) rats. Hemodynamic, histological, and vascular contractile studies were conducted in rats 4 weeks after bile duct ligation. An aqueous extract of Sm (0.2 g twice per day) or vehicle was administered for 4 weeks to BDL rats. Sm treatment in BDL rats significantly reduced histological grades of fibrosis and ameliorated the portal hypertensive state (including portal venous pressure, superior mesenteric artery blood flow, cardiac index, and total peripheral resistance) as compared with vehicle treatment. Moreover, Sm treatment enhanced the vascular sensitivity of mesenteric arteries to phenylephrine in BDL rats. Sm treatment had no effect on plasma biochemical profiles of either BDL or normal rats. Our results suggest that 4-week Sm treatment ameliorates the portal hypertensive state in BDL rats. Article: "Hemodynamic effects of Salvia miltiorrhiza on cirrhotic rats". Canadian

Journal of Physiology and Pharmacology. Volume 79, Number 7, July 2001, pp. 566-572(7) Salvia Miltiorrhiza has also been shown to be able to do the following:

• Activates kallikrein-kinin system in the (rat) kidney to promote production and secretion of prostaglandin E2, inducing dilation of renal vascular system, an increase in renal blood flow and glomerular filtration rate

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• Increases coronary blood flow without affecting heart rate

"Excessive oxidative stress is implicated in hepatic fibrogenesis. Extracts of Salvia miltiorrhiza (Sm) have been shown to protect cells against oxidative stress. In this study we investigated the in vitro and in vivo effects of Sm on hepatic fibrosis. A cell line of rat hepatic stellate cells (HSC-T6) was stimulated with transforming growth factor-beta1 (TGF-beta1). The inhibitory effects of Sm (50-400 microg/ml) on TGF-beta1-induced alpha-smooth muscle actin (alpha-SMA) secretion and the mRNA expressions of fibrosis-related genes, including alpha-SMA, connective tissue growth factor (CTGF), and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed. Fibrosis was induced by dimethylnitrosamine (DMN) administration in rats. DMN-treated rats were randomly assigned to 1 of 4 groups: saline, Sm (20 mg/kg), Sm (100 mg/kg), or silymarin (100 mg/kg), each given by gavage twice daily for 5 weeks starting from the onset of DMN administration. Sm (200 and 400 microg/ml) significantly inhibited TGF-beta1-stimulated alpha-SMA secretion and the mRNA expressions of alpha-SMA, CTGF, and TIMP-1 in HSC-T6 cells. Fibrosis scores of livers from DMN-treated rats with either a low (1.8 +/- 0.2) or high (1.8 +/- 0.1) dose of Sm, or silymarin (1.4 +/- 0.2) were significantly reduced in comparison with DMN-treated rats receiving saline (3.1 +/- 0.1). Hepatic collagen contents were also significantly reduced by either Sm or silymarin treatment. The mRNA expression levels of alpha-SMA, TGF-beta1, and procollagen I were all attenuated in Sm- and silymarin-treated rats. Moreover, levels of plasma aspartate transaminase activities were reduced by Sm and silymarin treatment. In conclusion, our results show that Sm exerted antifibrotic effects in both HSC-T6 cells and in rats with DMN-induced fibrosis." "Antifibrotic effects of Salvia

miltiorrhiza on dimethylnitrosamine-intoxicated rats". J. Biomed Sci. 2005;12(1):185-95.

"Acute liver damage from D-galactosamine was initiated in rats that were then treated with Paeonia lactiflora and Salvia miltiorrhiza. Parameters measured in this study were changes in ALT, bilirubin, levels of plasma fibronectin and pathological histology. The results showed that both herbs increased plasma fibronectin and improved the reticuloendothelial system function. Aggregation of albumin, collagen fragments and immune complexes were markedly reduced. Liver immune damage and micro-circulation disorders were avoided, justifying the important role of Paeonia lactiflora and Salvia miltiorrhiza in protecting hepatocytes". Citation : Qi, XG, "Protective mechanism of Salvia

miltiorrhiza and Paeonia lactiflora for experimental liver damage", Chung His Ho Tsa Chih,

11(2), pp 102-4, 1991.

"The preventive effect of Salvia miltiorrhiza extracts (SMEs) on the progress of bone loss induced by ovariectomy (OVX) was studied in rats. We measured body weight and bone histomorphometry in sham, OVX or SMEs-administered OVX rats. From light microscopic analyses, a porous or erosive appearances were observed on the surface of trabecular bone of tibia in OVX rats, whereas those of the same bone in sham rats and in SMEs-administered rats were composed of fine particles. The trabecular bone area and trabecular thickness in OVX rats decreased by 50% from those in sham rats, these decreases were completely inhibited by administration of SMEs for 7 weeks. In this study, the mechanical strength in femur neck was significantly enhanced by the treatment of SMEs for 7 weeks. In OVX rats, free T3 was normal in all cases, whereas free T4 was significantly increased. Although there was no difference between OVX and SMEs-administered rats in T3 level, we have found significant difference between them in T4 level. These results strongly suggest that SMEs are effective in preventing the development of bone loss induced by OVX in rats." "Prevention of bone

loss in ovariectomized rats: the effect of Salvia miltiorrhiza extracts." Immunopharmacol

Immunotoxicol. 2004 Feb;26(1):135-44.

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7. Radix Glycyrrhiza Glabra


• It is used to invigorate functions of the heart and spleen for the treatment of symptoms due to deficiency of vital energy of these viscera.

Research: Licorice has long been used to treat a variety of inflammatory conditions such as asthma, skin diseases (e.g., psoriasis or eczema), peptic ulcers, and even rheumatoid arthritis. Although for the most part good clinical studies in this area are lacking, the existing evidence for the anti-inflammatory effects of particularly GA at least support the therapeutic rationale for some of these treatments. Early reports from the late 1950s demonstrated that 2% topical preparations of GA were beneficial in inflammatory cutaneous disorders.40 Animal studies utilizing rodent models of inflammation during this period confirmed the efficacy of these clinical findings.41 More recently, Inoue et al. demonstrated the potent anti-inflammatory effect of GA on TPA-induced mouse ear edema.42 Both oral and topical preparations of GA when given 30 min before TPA treatment inhibited edema formation. A possible mechanism for these anti-inflammatory effects was offered by Shiki et al., who showed that GL stabilizes lysosomes by inhibiting phospholipase A activity in the lysosomal membrane.43 This prevented the release of proteolytic enzymes and acid phosphatases from lysosomes into damaged tissue. Another possible anti-inflammatory mechanism could involve GA’s ability to prevent mononuclear leucocyte migration into the injured tissue as shown in a carrageenan-induced rat paw edema model.44 An additional mechanism could involve GA inhibition of the human complement cascade as shown by Kroes et al.45

Anti-inflammatory Effects: The above-mentioned anti-inflammatory effects of GA and GL resemble cortisol’s effect on the inflammation process. Recent studies show that GA is a potent inhibitor of the skin enzyme 11-b-hydroxysteroid dehydrogenase (11-?OHSD) which catalyses the conversion of active cortisol into less active metabolites.46 Thus, GA likely acts by potentiating the known anti-inflammatory effects of cortisol. This idea is further supported by studies showing that GA, GL and carbenoxolone do not have anti-inflammatory effects in adrenalectomized animals, indicating that the effects are due to cortisol. This inhibition of 11-?OHSD appears to occur in most all tissues. In rats this enzyme activity was decreased in most organs after intraperitoneal injection of GA.47

Antitussive and Expectorant Effects: Licorice has through the years developed a reputation as an effective expectorant and cough suppressant chiefly used to treat the symptoms of the common cold. Licorice extracts are often found in a variety of throat lozenges. However, no clinical studies have been carried out supporting this widespread use. The cough suppressant effect could be related to its demulcent properties on irritated throat tissues. The well-documented protective effects on gastric mucosal cells could also occur in the upper esophagus and throat, perhaps explaining some of its claimed antitussive effects. The strong, sweet taste of GL could be partly responsible for its expectorant properties.

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40. Colin-Jones E. Glycyrrhetinic acid. Br Med J. 1957;161. 41. Finney R, Somers G. The anti-inflammatory activity of glycyrrhetinic acid and derivatives. J Pharm Pharmacol. 1958;10:613-620. 42. Inoue H, Mori T. Modulation by glycyrrhetinic acid derivatives of TPA-induced mouse ear oedema. B J Pharmacol. 1989;96:204-210. 43. Shiki Y, Ishikawa Y, et al. Effect of glycyrrhizin on lysosome labilization by phospholipase A2. Amer J Chin Med.1986;14 (3-4):131-137. 44. Capasso F, Mascoto N, et al. Glycyrrhetinic acid, leucocytes and prostaglandins. J Pharm Pharmacol. 1983;35:332-335. 45. Kroes B, Beukelman C, et al. Inhibition of human complement by b-glycyrrhetinic acid. Immunology. 1997;90(1):115-120. 46. Teelucksingh S, Mackie A, et al. Potentiation of hydrocortisone activity in skin by glycyrrhetinic acid. Lancet. 1990:1060-1063. 47. Marandici A, Monder C, et al. Inhibition by glycyrrhetinic acid of rat tissue 11-hydroxysteroid dehydrogenase in vivo. Steroids. 1993;58(4):153-156. "(R)-4-(3,4-Dihydro-8,8-dimethyl)-2H,8H-benzo[1,2-b:3,4-b']dipyran-3yl)-1,3-benzenediol

(glabridin), a flavonoid present in licorice extract, is known to have antimicrobial, anti-inflammatory, and cardiovascular protective activities. In the present study, we report the inhibitory effect of glabridin on nitric oxide (NO) production and inducible nitric oxide (iNOS) gene expression in murine macrophages. Glabridin attenuated lipopolysaccharide (LPS)-induced NO production in isolated mouse peritoneal macrophages and RAW 264.7 cells, a mouse macrophage-like cell line. Moreover, iNOS mRNA expression was also blocked by glabridin treatment in LPS-stimulated RAW 264.7 cells. Further study demonstrated that the LPS-induced nuclear factor (NF)-B/Rel DNA binding activity and NF- B/Rel-dependent reporter gene activity were significantly inhibited by glabridin in RAW 264.7 cells and that this effect was mediated through the inhibition of inhibitory factor- B degradation and p65 nuclear translocation. Moreover, reactive oxygen species generation was also suppressed by glabridin treatment in RAW 264.7 cells. In contrast, the activity of mitogen-activated protein kinases

was unaffected by glabridin treatment. In animal model, in vivo administration of glabridin increased the rate of survival of LPS-treated mice and inhibited LPS-induced increase in plasma concentrations of nitrite/nitrate and tumor necrosis factor- . Collectively, these data suggest that glabridin inhibits NO production and iNOS gene expression by blocking NF- B/Rel activation and that this effect was mediated, at least in part, by inhibiting reactive oxygen species generation. Furthermore, in vivo anti-inflammatory effect of glabridin suggests a possible therapeutic application of this agent in inflammatory diseases." "Glabridin, an Isoflavan from Licorice Root, Inhibits Inducible Nitric-

Oxide Synthase Expression and Improves Survival of Mice in Experimental Model of Septic

Shock" Journal of Pharmacology And Experimental Therapeutics Fast Forward First published

on November 10, 2004; DOI: 10.1124/jpet.104.077107 For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/5-Elements_Testimonials.htm

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2) Adam’s Pride


1. Improve and enhance men’s vitality 2. Erectile Dysfunction 3. Strengthen bodily functions 4. Improve men’s .appetite. 5. Remove tiredness after sexual activity 6. Sexual enhancement

Herbs:

Radix Morinda Officinalis Radix Achyranthis Bidentatae Rehmannia Root Cornus Officinalis Ligusticum ChuanXiong Panax Quinquefolium Radix Dipsaci Paeonia Lactiflora 1. Radix Morinda Officinalis

Function of the herb: Ÿ It is used to reinforce the vital function of the kidneys especially that of sexual organs

for the treatment of impotence and premature ejaculation of men. a) It is used to reinforce the vital function of the kidneys especially that of sexual organs for the treatment of impotence and premature ejaculation of men. The herb is pungent and sweet in flavour, slightly warm in nature, it is tropistic to the liver and kidney channels. Being sweet and warm, it can invigorate kidney-yang; as a pungent and warm agent, it is capable of dispelling wind-dampness. Being slightly warm, moist and dry, it has effects of invigorating Yang and supplementing vital essence and is especially effective in strengthening muscles and bones, serving to treat insufficiency of kidney-yang and deficiency-cold of essence and blood marked by impotence, infertility and athralgia due to wind-dampness. It tonifies the kidney, invigorating Yang, strengthening muscles and bones, dispelling wind and eliminating dampness. Source: "Legendary Chinese Healing Herbs" -

Dr. Henry Lu- Academy of Oriental Heritage, Blaine, USA. The author is best known for his

translation of "Yellow Emperor's Classics of Internal Medicine" written around 300 B.C. from

Chinese to English; "Dictionary Of Traditional Chinese Medicine" written by Prof. Lou Zhicen

- Professor of Pharmacognosy, Prof. Li Shuncheng - Associate Professor of Medicine and Prof.

Tang Zijin - Associate Professor of Medicine of Beijing Medical College.

b) Research showed that it benefits the reproductive organs A 30g/kg/day dose of Radix Morindae Officinalis decoction was administered to castrated mice once a day for 15 days. Results showed that the weights of levator ani muscles, seminal vesicle and prostate were not significantly effected. Qiao

Zhisheng, et al. Comparison of pharmacological activities of different Radix Morindae

Officinalis species, Chinese Journal of Integrated Traditional and Western Medicine. 1991;

11(7):415.

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2. Radix Achyranthes Bidentatae

Function of the herb: • Nourish liver, kidney and strengthen sinew and bones • Treats aching backs and knees • Treats asthenia (loss of strength/debility) of lower limbs • Regulates and activates blood • Tones up liver and kidneys

Researches by scientists have shown that Radix Achyrantes can be used to improve bone quality. Bones are forms from bone marrows. Bone marrows come from kidney essence. One of the reasons why men suffer from ED problems is because of weakening kidneys. One of the phyto-chemicals of Achyrantes is beta-sitosterol. Researches by scientists have discovered that beta-sitosterol reduces blood levels of cholesterol, and is sometimes used in treating hypercholesterolemia (high level of cholesterol in the blood. It is a well known fact high cholesterol can cause ED problems. Beta-sitosterol also prevents the oxidation of LDL cholesterol thereby reducing the risk of atherosclerosis. a) Bioassay-directed fractionation of a butanol-soluble fraction of methanol extract of the root of Achyranthes bidentata resulted in the isolation of 5 new oleanolic acid glycosides 1-5, namely, 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester (1), achyranthoside C dimethyl ester (2), achyranthoside C butyl dimethyl ester (3), achyranthoside E dimethyl ester (4), and achyranthoside E butyl methyl ester (5), together with 10 known compounds. Their structures were established on the basis of spectroscopic interpretation and chemical methods. All the oleanolic acid glycosides inhibited the formation of osteoclast-like multinucleated cells (OCLs) induced by 1alpha,25(OH)2D3 in a co-culture assay system. (An osteoclast (from the Greek words for "bone" and "broken") is a type of bone cell that removes bone tissue by removing the bone's mineralized matrix. This process is known as bone resorption. Osteoclasts and osteoblasts are instrumental in controlling the amount of bone tissue. Osteoblasts form bone; osteoclasts resorb bone. Osteoclasts are formed by the fusion of cells of the monocyte cell line. Osteoclasts are characterized by high expression of tartrate resistant acid phosphatase (TRAP) and cathepsin K.). "Five new oleanolic acid glycosides from Achyranthes

Bidentata with inhibitory activity on osteoclast formation." Planta Med 2005 July 71 (7): 673-9

b) Eight compounds were separated from the roots of Achyrathes bidentata by repeated chromatography. On the basis of physicochemical properties and spectral analysis their structures were elucidated as alpha-spinasterol (1), beta-sitosterol (2), chrysophanol (3), dibutyl phthalate (4), palmitic acid (5), alpha-spinasterol-3-O-beta-D-glucoside (6), daucosterol (7) and ecdysterone (8). Compounds 1-7 were isolated from the plant for the first time. “Separation and identification of the

compounds from Achyranthes bidentata Bl”: Zhongguo Zhong Yao Za Zhi 1997

May;22(5):293-5, 319-20. c) Beta-sitosterol (BS) is a compound that has shown various activities potentially useful for human health. In the present study, we determined its antigenotoxic capacity and lymphocyte induction potential in mouse as well as its capacity to trap free radicals in vitro. BS, in doses from 200 to 1,000 mg/kg, was able to significantly reduce the frequency of sister chromatid exchanges induced by 10 mg/kg of doxorubicin (DX) in bone marrow cells. The same range of BS doses also gave rise to a strong reduction in the rate of micronucleated, polychromatic erythrocytes induced by DX. In addition, we determined an increase in the production of lymphocytes in mice administered with BS. By means of the DPPH assay, the compound was shown to trap free radicals in a concentration dependent manner as high as 78.12% using 250 µg/ml. Our research established three relevant biological activities of BS which show its potential as a chemopreventive (The use of chemical agents, drugs, or food supplements to prevent disease) agent. “Cell protection induced by beta-

sitosterol: inhibition of genotoxic damage, stimulation of lymphocyte production, and

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determination of its antioxidant capacity”. Archives of Toxicology DOI 10.1007/s00204-007-

0277-3

d) Research by scientists showed that beta-sitosterol decreases cholesterol synthesis. CaCo-2 cells were used to address the effect of the plant sterol, beta-sitosterol, on cholesterol trafficking, cholesterol metabolism, and apoB secretion. Compared to cells incubated with micelles (5mM taurocholate and 250 microM oleic acid) containing cholesterol, which caused an increase in the influx of plasma membrane cholesterol to the endoplasmic reticulum and increased the secretion of cholesteryl esters derived from the plasma membrane, beta-sitosterol did not alter cholesterol trafficking or cholesteryl ester secretion. Including beta-sitosterol in the micelle together with cholesterol attentuated the influx of plasma membrane cholesterol and prevented the secretion of cholesteryl esters derived from the plasma membrane. Stigmasterol and campesterol had effects similar to beta-sitosterol although campesterol did not promote a modest influx of plasma membrane cholesterol. Including beta-sitosterol in the micelle with cholesterol decreased the uptake of cholesterol.Compared to cholesterol, 60% less beta-sitosterol was taken up by CaCo-2 cells. No observable esterification of beta-sitosterol was appreciated and the transport of the plant sterol to the basolateral medium was negligible. Cholesterol synthesis and HMG-CoA reductase activities were decreased in cells incubated with beta-sitosterol. This was associated with a decrease in reductase mass and mRNA levels. Cholesteryl ester synthesis and ACAT activities were unaltered by beta-sitosterol. Both stigmasterol and campesterol decreased reductase activity, but only campesterol increased ACAT activity. Beta-sitosterol did not affect the secretion of apoB mass. The results suggest that beta-sitosterol does not promote cholesterol trafficking from the plasma membrane to the endoplasmic reticulum. Beta-sitosterol interferes with the uptake of micellar cholesterol causing less plasma membrane cholesterol to influx and less cholesteryl ester to be secreted. Despite its lack of effect on cholesterol trafficking, beta-sitosterol decreases cholesterol synthesis at the level of HMG-CoA reductase gene expression. "Effect of micellar beta-sitosterol on cholesterol metabolism in

CaCo-2 cells." Journal of Lipid Research, Vol 38, 348-360

e) Some of the researches done on the relationship between beta-sitosterol and cholesterol:

i) At McGill University in Montreal (Can. J. Physiol. Pharmacol. 75, 1997, p. 217-27) doctors did a review of the literature on beta-sitosterol and cholesterol metabolism. They researched in detail 18 major studies that used sitosterols to lower cholesterol and triglycerides. They concluded, "addition to diet of phytosterols represents an effective means of improving circulating lipid profiles to reduce risk of coronary heart disease." This study came complete with forty high quality references and left no doubt about the effectiveness of phytosterols on humans. Also at McGill University (Metabolism Clinic Experiments 47, 1998, p. 751-6) patients on a fixed diet were given sterols from pine oil for a mere ten days in a strict, randomized crossover study. This was not a low fat or low cholesterol diet at all. They successfully lowered both their total cholesterol and LDL levels in this short term placebo controlled experiment. They concluded, these results demonstrate the short term efficacy of pine oil plant sterols as cholesterol lowering agents" ii) A very interesting study was done at the Center for Human Nutrition in France (Ann. Nutr. Metab. 39, 1995, p. 291-5) in that healthy people with normal cholesterol levels were given beta- sitosterol to see if their normal levels could be lowered even further. We always think of studies as using unhealthy people with pathological cholesterol levels given supplements to make them normal again. Amazingly enough the healthy people lowered their normal cholesterol levels even more with no change in diet or exercise. In fact, they were a full 10% lower in only a month. This kind of effect is really fascinating. They said, "The present results may be of great interest in the prevention of high cholesterol diet-associated risks, especially in the prevention of cardiovascular diseases. Since beta-sitosterol was so effective for people who didn't even need it, think what it will do for those people who do need to lower their blood lipids. They concluded, "These findings suggest that a significant lowering of plasma total and LDL cholesterol can be effected by a modest dietary intake of soybean phytosterols"

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iii) A good study was done at the Wageningen Agricultural Institute in the Netherlands, the same clinic that did so much good research on trans fatty acids (Am. J. Clin. Nutr. 72, 2000, p. 1510-5). They gave men and women a margarine containing plant sterols and got very significant reductions in cholesterol as well as lower LDL levels in only three weeks. Why a clinic would give margarine to people after studying the negative effects of hydrogenated oils is another matter. Again, these were healthy subjects with normal cholesterol levels, yet they still got great benefits very quickly with no change in diet or exercise. iv) At Uppsala University in Sweden (Eur. Heart J. Supp. 1, 1999, p. S80-S90) the doctors wanted to give the volunteers the phytosterols in conjunction with a cholesterol lowering diet to see the results of a more comprehensive lifestyle program. The results were really impressive in that the men and women lowered total cholesterol a full 15% and LDL cholesterol a full 19% in less than a month. The shows the very dramatic results you can get with just adding some reasonable dietary changes even without any exercise program at all. v) At the University of British Columbia at their St. Paul's Hospital (American Journal of Medicine 107 (1999) p. 588-94) a very impressive review was done complete with 86 references of using plant sterols to lower total cholesterol and LDL. They said of the recent research, "In 16 recently published human studies that used phytosterols to decrease plasma cholesterol levels in a total of 590 subjects, phytosterol therapy was accompanied by an average 10% decrease in total cholesterol and 13% decrease in LDL cholesterol levels." This is the best review to date and should convince anyone of the effectiveness of sterols over drugs. vi) At the University of Kagawa in Tokyo two studies were done. The first was done on healthy young men who were given plant sterols for only five days. In this short time their cholesterol levels fell measurably (Joshi Eiyo Daigaku Kiyo 14, 1983, p. 165-72). The second study was done on healthy young women (same journal 15, 1984 p. 11-18) again giving them plant sterols for only five days. "Administration of phytosterol (mainly sitosterol) increased the output of fecal cholesterol." These were all healthy young Japanese people eating a traditional low-fat diet who did not have a cholesterol problem to begin with, yet they received measurable results in only five days. vii) At the University of California in San Diego men were isolated in a hospital ward and fed 350 mg of cholesterol and then beta-sitosterol supplements (American Journal of Clinical Nutrition 35, 1982, p. 697-700). This resulted in a 42% decrease in cholesterol absorption in the intestines. They said, "Evidently, the judicious addition of beta-sitosterol to meals containing cholesterol rich foods will result in a decrease in cholesterol absorption with a consequent decrease in plasma cholesterol" viii) The University of Helsinki took a big interest in lowering cholesterol with plant sterol therapy back in 1988. The first study (Clinical Chimica Acta 178, p. 41-9) studied familial (genetic) hypercholesteremia. The higher the sterol levels they found in the patients blood the more cholesterol was excreted rather than absorbed. The second study was in 1989 (Metabolism Clinical Experiments 38, p. 136-40). Men were studied again for blood levels of sterols and they found the higher the levels the more cholesterol was excreted successfully. The third study in 1994 (American Journal of Clinical Nutrition 59, p. 1338-46) studied vegetarians who eat twice as many plant sterols as normal people. They showed one reason vegetarians have lower cholesterol levels besides the food they eat is the efficiency of their cholesterol excretion due to their intakes of plant sterols. In the last study in 1999 (Current Opinion Lipidology 10, p. 9-14) they said, "Plant sterols may be useful for the treatment of hyper-cholesteremiaãƒ•hey may have a potent cholesterol lowering effect as shown in normal and hypercholesteremic men and women with and without coronary heart disease and diabetes mellitus" ix) The best study of all was a review from the University of British Columbia (American Journal of Medicine 107, 1999, p. 588-94). This included a full 86 references, and went over seventeen different human studies using plant sterols to lower cholesterol since 1951 (Proceedings of the Society for Biological Medicine 78, 1951, p. 143-7). A total of 590 men and women were used in these studies with phytosterol therapy resulting in an average 10% reduction in total cholesterol and a 13%

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reduction in LDL cholesterol. They found this worked best in high-fat diets; the worse the diet the more results the researchers got. 3. Radix Rehmannia Glutinosa


Ÿ It is used to nourish the vital essence Ÿ It is an effective blood tonic a) Research by scientists showed that Rehmannia improves blood movement. Effects of 50% ethanolic extract (JR-ext) from Chinese Rehmanniae Radix (the steamed and dried root of Rehmannia glutinosa, "Jyuku-Jio" in Japanese) on the hemorheology of inflammatory, thrombosic and intact animals were examined in the in vivo models. JR-ext (200 mg/kg, p.o.) inhibited the reduction of fibrinolytic activity and erythrocyte deformability, the decrease in erythrocyte counts and the increase in connective tissue of the thoracic artery in a chronic inflammatory model, adjuvant-induced arthritis. However, JR-ext was ineffective on the development of edema in the arthritic rats and on acute and chronic inflammation. JR-ext inhibited the reduction of erythrocyte deformability, but not the decrease of coagulative factors in a thrombosic model, endotoxin-induced disseminated intravascular coagulation (DIC). JR-ext also showed a promoting effect on erythrocyte deformability and fibrinolytic activity in intact rats. These results suggest that orally administered JR-ext can prevent an inducement of impediment in the peripheral microcirculation of various chronic diseases through the improvement of hemorheology (blood movement). “Studies on rehmanniae radix. I. Effect of 50%

ethanolic extract from steamed and dried rehmanniae radix on hemorheology in arthritic and

thrombosic rats.” Biol Pharm Bull (1994) 17: 1282-6.

3. Fructus Cornus Officinalis


Ÿ It is used to replenish the vital essence of the liver and kidneys In our previous study, we reported the renoprotective effect of Corni Fructus (Cornus officinalis SIEB. et ZUCC. In this study, we investigated the effects of morroniside isolated from Corni Fructus on renal damage in streptozotocin-treated diabetic rats. Oral administration of morroniside at a dose of 20 or 100 mg/kg body weight/d for 20 d to diabetic rats resulted in significant decreases in increasing serum glucose and urinary protein levels. Moreover, the decreased levels of serum albumin and total protein in diabetic rats were significantly increased by morroniside administration at a dose of 100 mg/kg body weight/d. In addition, morroniside significantly reduced the elevated serum urea nitrogen level and showed a tendency to reduce creatinine clearance. Morroniside also significantly reduced the enhanced levels of serum glycosylated protein, and serum and renal thiobarbituric acid-reactive substances. Protein expressions related to the advanced glycation endproduct (AGE) level and actions, oxidative stress such as N(epsilon)-(carboxyethyl)lysine, as well as receptors for AGE and heme oxygenase-1 were increased in diabetic rats, but the levels were also significantly decreased by the administration of morroniside. This suggests that morroniside exhibits protective effects against diabetic renal damage by inhibiting hyperglycemia and oxidative stress. These results indicate that morroniside is one component partly responsible for the protective effects of Corni Fructus and Hachimi-jio-gan against diabetic renal damage. “Protective Effects of Morroniside Isolated from

Corni Fructus against Renal Damage in Streptozotocin-Induced Diabetic Rats.” Biol Pharm

Bull. 2008 Jul;31(7):1422-8

b) Clinical results demonstrate that Fructus corni can promote a protective antioxidant defense

state by affecting some important enzymatic and nonenzymatic oxidant-scavenging systems and

may thus be useful for the prevention or treatment of disorders associated with oxidative

damage in the kidneys. The present study determined the effects of Fructus corni extract (FCE) on the levels of hydrogen peroxide (H2O2) and superoxide anion (O2-), on the glutathione (GSH) redox cycle and on the activities of antioxidant enzymes in bovine pulmonary artery endothelial cells

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(PAECs). 2. Confluent monolayers of PAECs were incubated with FCE, and oxidative stress was triggered by hypoxanthine and xanthine oxidase (to induce H2O2) or H2O2 (to induce O2-). 3. FCE exhibited a concentration-dependent suppression of H2O2 and O2-. 4. It modulated the GSH redox cycle by increasing the intracellular GSH content, the activities of GSH peroxidase and GSH disulfide reductase, and by decreasing the intracellular level of GSH disulfide. 5. It also increased the activities of superoxide dismutase and catalase. 6. These results demonstrate that FCE can promote a protective antioxidant defense state by affecting some important enzymatic and nonenzymatic oxidant-scavenging systems and may thus be useful for the prevention or treatment of disorders associated with oxidative damage. “Fructus corni enhances endothelial cell antioxidant defenses”. Gen

Pharmacol. 1998; 31(2):221-5

5) Rhizoma Ligusticum Chuanxiong


Ÿ It is used to invigorate blood circulation and promote the flow of vital energy a) Ligustilide is the most abundant bioactive ingredient in Rhizoma Chuanxiong, a Chinese medicinal herb commonly used for the treatment of cardiovascular ailments. The present study reported, for the first time, the pharmacokinetics of ligustilide, administered in its pure form and in an herbal extract, in rats. After i.v. administration of pure ligustilide, it was distributed extensively (Vd, 3.76 ± 1.23 l/kg) and eliminated rapidly (t1/2,

0.31 ± 0.12 h). The i.v. clearance (CL) of ligustilide after Chuanxiong extract administration was significantly higher than that dosed in its pure form [CL, 20.35 ± 3.05 versus 9.14 ± 1.27 l/h/kg, p < 0.01; area under the curve (AUC), 0.79 ± 0.10 versus 1.81 ± 0.24 mg !

h/l, p < 0.01], suggesting significant interaction between ligustilide and components present in the extract. Dose-dependent pharmacokinetics was observed after i.p. administration, and a significantly higher dose-normalized AUC (1.77 ± 0.23 mg ! h/l) at 52 mg/kg was obtained than that at 26 mg/kg

(0.93 ± 0.07 mg ! h/l, p < 0.05). Oral bioavailability of ligustilide was low (2.6%), which was partly because of extensive first-pass metabolism in the liver. Seven metabolites of ligustilide were identified, and three of them were unequivocally characterized as butylidenephthalide, senkyunolide I, and senkyunolide H. These three compounds also occurred naturally in the herb and were reported to be bioactive. “Pharmacokinetics and Metabolism of Ligustilide, a Major Bioactive Component

in Rhizoma Chuanxiong, in the Rat.” Drug Metabolism and Disposition DOI:

10.1124/dmd.107.017707

b) The purpose of the present study was to investigate the effect of ligustilide on vasodilatation in rat mesenteric artery and the mechanisms responsible for it. Isometric tension of rat mesenteric artery rings was recorded by a sensitive myograph system in vitro. The results showed that ligustilide at concentrations more than 10 µM relaxed potassium chloride (KCl)-preconstricted rat mesenteric artery in a concentration-dependent manner. The vasodilatation effect of ligustilide was not dependent on endothelium. Ligustilide rightwards shifted concentration-response curves induced by KCl, calcium chloride (CaCl2), noradrenaline (NA) or 5-hydroxytryptamine (5-HT) in a non-parallel manner. This suggests that the vasodilatation effects were most likely via voltage-dependent calcium channel (VDCC) and receptor-operated calcium channel (ROCC). Propranolol, glibenclamide, tetraethylammonium and barium chloride did not affect the vasodilation induced by ligustilide, showing that p-adrenoceptor, ATP sensitive potassium channel, calcium-activated potassium channel and inwardly rectifying potassium channel were not involved in the vasodilatation. Ligustilide concentration-dependently inhibited the vasoconstriction induced by NA or CaCl2 in Ca2+-free medium, indicating that the vasodilatation relates to inhibition of extracellular Ca2+ influx through VDCC and ROCC, and intracellular Ca2+ release from Ca2+ store. Since caffeine-induced contraction was inhibited by ligustilide, inhibition of intracellular Ca2+ released by ligustilide occurred via the ryanodine receptors. Our results suggest that ligustilide (from Rhizoma Ligusticum Chuanxiong) induces vasodilatation in rat mesenteric artery by inhibiting the VDCC and ROCC, and receptor-mediated Ca2+ influx and release. “Ligustilide induces vasodilatation (relaxes the smooth muscle

in blood vessels) via inhibiting voltage dependent calcium channel and receptor-mediated Ca2+

influx and release.” Vascular Pharmacology ISSN 1537-1891 2006, vol. 45, n 3, pp.171-176

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c) Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5"105 µl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6±4.6% of the control to 12.5±2.1% in the presence of TMP (1.46 mM) and SAB (10 µM) (P<0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress. “Inhibition of shear-induced platelet aggregation in rat by tetramethylpyrazine and salvianolic

acid B, active component of Rhizoma Ligustici Chuanxiong”. Clinical Hemorheology and

Microcirculation Vol. 31, Number 2/2004 Pages 97-103

6. Radix Dipsacus Asper


Ÿ It is used to replenish and tonifys the liver and kidneys

The methanol extract from Dipsacus asper Wall (Dipsacaceae) was found to show antioxidant activity against free radical and Cu(2+)-mediated LDL oxidation. In further study, to identify active constituents from the plant, six caffeoyl quinic acid derivatives: 3,4-di-O-caffeoylquinic acid (1), methyl 3,4-di-O-caffeoyl quinate (2), 3,5-di-O-caffeoylquinic acid (3), methyl 3,5-di-O-caffeoyl quinate (4), 4,5-di-O-caffeoylquinic acid (5) and methyl 4,5-di-O-caffeoyl quinate (6) were isolated. Their structures were identified by spectroscopic methods including 2D-NMR. The isolated compounds, 1-6, were found to be potent scavengers of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), and are more potent than butylated hydroxyl toluene (BHT) used as a positive control. The compounds 1-6 also inhibited Cu(2+)-mediated low-density lipoprotein (LDL) oxidation. They increased the lag time of conjugated dienes formation and inhibited the generation of thiobarbituric acid reactive substances (TBARS) in a dose-dependent manner. These results suggested that Dipsacus asper due to its antioxidant constituents, 1-6, may have a role to play in preventing the development and progression of atherosclerotic disease. “The antioxidant activity of caffeoyl quinic acid from

the roots of Dipsacus asper.” J Ethnopharmacol. 2006 May 22; : 16809011

7. Radix Paeonia Lactiflora


• Used to nourish the blood • Used as a spasmodic and pain relieving agent for headaches, abdominal pains, spasms of the

calf, muscles etc.

a) Paeonia lactiflora and Astragalus membranaceus are two popular traditional Chinese medicines, commonly used in Chinese herb prescription to treat liver disease. The extract prepared from the roots of Paeonia lactiflora and Astragalus membranaceus (PAE) demonstrated more excellent hepato-protective activity than the single herbs used individually as indicated in our preliminary studies. The present study was carried out to investigate the effects of PAE on liver fibrosis in rats induced by carbon tetrachloride (CCl4) and to explore its possible mechanisms. Liver fibrosis was induced in male Sprague–Dawley rats by injection with 50% CCl4 subcutaneously twice a week for 8 weeks. At the same time, PAE (40, 80 and 160 mg/kg) was administered intragastrically. Upon pathological examination, the PAE-treated rats significantly reduced the liver damage and the symptoms of liver fibrosis. Administration of PAE decreased CCl4-induced elevation of serum transaminase activities, hyaluronic acid, laminin and procollagen type III levels, and contents of hydroxyproline in liver tissue by approximately 30–60%. It also restored the decrease in SOD and GSH-Px activites and inhibited

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the formation of lipid peroxidative products during CCl4 treatment. Moreover, PAE (80, 160 mg/kg, ig) decreased the elevation of TGF-#1 by 47.7% and 53.1%, respectively. In the primary cultured hepatic stellate cells (HSCs), PAE also significantly decreased [3H] thymidine incorporation in cells stimulated with platelet-derived growth factor-B subunit homodimer (PDGF-BB) and suppressed [3H] proline incorporation. These results suggested that PAE significantly inhibited the progression of hepatic fibrosis induced by CCl4, and the inhibitory effect of PAE on hepatic fibrosis might be associated with its ability to scavenge free radicals, decrease the level of TGF-#1 and inhibit collagen synthesis and proliferation in HSCs. “Effects and mechanisms of extract from Paeonia lactiflora

and Astragalus membranaceus on liver fibrosis induced by carbon tetrachloride in rat.”

Journal of Enthnopharmacology Volume 112, Issue 3 25 July 2007, Pages 514-523

b) This study examined the effect of Paeoniae Radix (PR) on endothelial function and the activity of superoxide dismutase (SOD) of erythrocytes in rats administered a high-fat diet. Administration of the extract of PR increased the endothelium-dependent relaxation and the activities of SOD compared with high the cholesterol diet group significantly. Hypercholesterolaemia induced an increase of endothelial superoxide anion and endothelial dysfunction. Paeoniae Radix is suggested to have a protective effect on endothelial cells and their function. “Effect of extract prepared from the roots

of Paeonia lactiflora on endothelium-dependent relaxation and antioxidant enzyme activity in

rats administered high-fat diet.” (The endothelium is the thin layer of cells that line the interior

surface of blood vessels. Endothelial cells line the entire circulatory system, from the heart to

the smallest capillary. These cells reduce turbulence of the flow of blood allowing the fluid to be

pumped farther.) Phytother Res. 1999 Sep;13(6):526-8

c) Paeoniae Radix (the roots of Paeonia lactiflora Pallas) is a crude drug that is used in Asia and Europe to improve blood flow. We studied its vasodilator effect and mechanisms of action in vitro. The extract from Paeoniae Radix (PRE) relaxed prostaglandin F2a-precontracted aortic ring preparations of isolated rat aorta that contained endothelium. Relaxation by PRE did not occur in specimens without endothelium, and was inhibited by pretreatment with 10-4 M NG-nitro-1-arginine methyl ester. Paeoniflorin and paeonol, the main active components of Paeoniae Radix, lacked a vasodilator effect. The effect of the component gallotannin was examined after treating PRE with tannase, but the product lacked a vasodilator effect. Pentagalloylglucose, hexagalloylglucose, heptagalloylglucose, and octagalloylglucose were extracted from PRE ; they relaxed aortic rings with endothelium, but failed to relax aortic rings without endothelium. We conclude that PRE exhibits an endothelium-dependent vasodilator effect on isolated rat aorta. “Endothelium-dependent

vasodilator effect of extract prepared from the roots of Paeonia lactiflora on isolated rat aorta.”

Planta medica ISSN 0032-0943 1996, vol. 62, n 5, pp. 436-439 (21 ref.)

d) Research showed that the herb: 1) Lower cholesterol levels. “Zhang Y. The effects of nifedipine, diltiazem, and Paeonia lactiflora

Pall. on atherogenesis in rabbits. Chung Hua Hsin Hsueh Kuan Ping Tsa Chih 1991;19:100–3” 2) Improve liver function and help prevent liver damage from chemical toxins such as carbon

tetrachloride. “Qi XG. Protective mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage. Chung Hsi I Chieh Ho Tsa Chih 1991;11:69, 102–4”

3) Inhibit blood platelet aggregation. “Liu J. Effect of Paeonia obovata 801 on metabolism of thromboxane B2 and arachidonic acid and on platelet aggregation in patients with coronary heart disease and cerebral thrombosis. Chung Hua I Hsueh Tsa Chih (Chin Med J) 1983;63:477–81.”

e) Clinical research by western scientists confirmed the validity of the historical use of the drug,

Radix Paeonia Lactiflora for improving liver functions by preventing liver damage and micro-

circulation disorders. "Acute liver damage from D-galactosamine was initiated in rats that were then treated with Paeonia lactiflora and Salvia miltiorrhiza. Parameters measured in this study were changes in ALT, bilirubin, levels of plasma fibronectin and pathological histology. The results showed that both herbs increased plasma fibronectin and improved the reticuloendothelial system function. Aggregation of albumin, collagen fragments and immune complexes were markedly reduced. Liver immune damage and micro-circulation disorders were avoided, justifying the

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important role of Paeonia lactiflora and Salvia miltiorrhiza in protecting hepatocytes". Protective

mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage. Zhong

Xi Yi Jie He Za Zhi 1991 Feb;11(2):102-4, 69. PMID: 2060049

For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/Adams%20Pride_Testimonials.htm

3) Alco-Liv


1. Liver cirrhosis (hardened liver) 2. Hepatomegaly (enlarged liver and spleen) 3. Adrenal fatigue (our herbs induce production of cortisone. Cortisone helps in the relief of stress,

physical neurogenic and physiological) 4. Liver detoxification (removes heavy metals like lead, mercury, cadmium, cobalt and radium) 5. Promotes bile production and excretion 6. Softens and shrinks liver and spleen 7. Increases red blood cells production 8. Treats fatty liver 9. Tired Eyes (For those who have dark circle under the

eyes they should add SpitalCare)

Herbs:

Radix Bupleurum Scorzonerifolium Lysimachia Christinae Artemisa Scoparia Radix Glycyrrhiza Glabra Scutellaria Baicalensis 1. Herba Lysimachia Christinae


• Counteract toxic effects to clear up liver heat expel urinary stones and promote urination

2. Radix Scutellaria Baicalensis


• It is used to protect the liver • It is used to induce liver regeneration • It is used for the treatment of pyogenic infection. It is used for febrile diseases

Research by western scientists has shown that this herb has anti-inflammatory and anti-cancer properties. The article wrote "Scutellaria baicalensis is a widely used Chinese herbal medicine that has been used historically in anti-inflammatory and anticancer therapy. The purpose of this study is to verify its anticancer activity on head and neck squamous cell carcinoma (HNSCC) in

vitro and in vivo and to investigate its effect on cyclooxygenase-2 (COX-2), which converts arachidonic acid to prostaglandin E2

(PGE2) and is highly expressed in HNSCC. Two human HNSCC cell lines (SCC-25 and KB) and a nontumorigenic cell line (HaCaT) were tested in vitro for growth inhibition,

proliferation cell nuclear antigen expression, and COX-2 activity and expression after treatment with Scutellaria baicalensis extract. Its effects were compared with those of baicalein (a flavonoid isolated

from Scutellaria baicalensis), indomethacin (a nonselective COX inhibitor), and celecoxib (a selective COX-2 inhibitor). Four nude mice with s.c. inoculation of KB cells were tested for its anticancer activity in vivo by oral administration of

Scutellaria baicalensis at a dose of 1.5 mg/mouse (75 mg/kg), five times/week for 7 weeks. Scutellaria baicalensis and other agents demonstrated a strong

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growth inhibition in both tested human HNSCC cell lines. No growth inhibition of HaCaT cells was observed with Scutellaria baicalensis. The IC50s were 150

$g/ml for Scutellaria baicalensis, 25 $M for celecoxib, and 75 $M for baicalein and indomethacin. Scutellaria

baicalensis, as well as celecoxib

and indomethacin, but not baicalein, suppressed proliferation cell nuclear antigen expression and PGE2 synthesis in both cell types. Scutellaria baicalensis

inhibited COX-2 expression, whereas celecoxib inhibited COX-2 activity directly. A 66% reduction in tumor mass was observed in the nude mice. Scutellaria baicalensis selectively and effectively inhibits cancer cell growth in vitro and in vivo and can be an effective chemotherapeutic agent for HNSCC. Inhibition of PGE2 synthesis via suppression of COX-2 expression may be responsible for its anticancer activity. Differences in biological effects of Scutellaria baicalensis compared with baicalein suggest the synergistic effects among components in Scutellaria baicalensis." "Inhibition of Cancer Cell Proliferation and Prostaglandin E2 Synthesis

by Scutellaria Baicalensis." Cancer Research 63, 4037-4043, July 15, 2003 American Association

for Cancer Research Western scientists have proven that this herb can be used to induce liver regeneration. The article wrote "The aim of this study is to elucidate the effects of Scutellaria

baicalensis Georgi (SbG) extract

and its constituents on macrophage-hepatocyte interaction in primary cultures. By using trans-well primary Kupffer cell culture or conditioned medium (CM) from murine macrophage RAW264.7 cell line (RAW cells), effects of SbG on hepatocyte growth were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide and trypan blue exclusion assay. Cytokine production, antibody-neutralization studies, and molecular mechanisms of transforming growth factor (TGF)-ß1 gene expression were elucidated on SbG-treated RAW264.7 cells. In addition, recombinant human TGF-ß1 (r-human TGF-ß1) was added to elucidate the mechanisms of SbG effects on cultured hepatocytes. Immunohistochemistry using anti–NF- B antibody was used to determine the possible signal transduction pathways in primary hepatocyte culture. The results showed that SbG stimulated the proliferation of cultured hepatocytes, possibly through NF- B, but not of Toll-like receptor 4 activation; whereas SbG-RAW-CM and SbG in trans-well significantly suppressed the proliferation of hepatocytes. Antibody-neutralization studies revealed that TGF-ß1 was the main antimitotic cytokine in SbG-treated RAW cells CM. The growth stimulation effect of SbG on cultured hepatocytes was inhibited by exogenous administration of r-human TGF-ß1. Furthermore, SbG induced NF-kB translocation into the nuclei of cultured cells. In the RAW264.7 line, SbG and baicalin stimulated TGF-ß1 gene expression via

NF- B and protein kinase C activation. We conclude that SbG stimulates

hepatocyte growth via activation of the NF- B pathway and induces TGF-ß1 gene expression through the Kupffer cell–hepatocyte interaction, which subsequently results in the inhibition of SbG-stimulated hepatocyte growth." "Effects of Scutellaria baicalensis Georgi on Macrophage-

Hepatocyte Interaction Through Cytokines Related to Growth Control of Murine Hepatocytes"

Experimental Biology and Medicine 231:444-455 (2006) Society for Experimental Biology and

Medicine 3. Radix Bupleurum Scorzonerifolium (Who's Firewood).

Function of the herb: • Disperse liver energy • Protect liver • Promote liver bile production • Treat fatty liver • Soften and shrink liver and spleen • It is rich in saponins that reduce inflammation, regulate hormone level and is used for liver

problems. 4. Folium Artemisa Scoparia

Function of the herb: • It is used to eliminate damp heat in the liver and jaundice caused by hepatitis

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• It is used to protect the liver • It is used to promote liver bile production and excretion • It is used as an antiviral herb • It is used as an adjuvant herb to treat liver and gall bladder disease

Research by western scientists has shown that this herb is effective for treating hepatitis. The article wrote "Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-gamma/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-gamma/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression." "Scoparone from Artemisia capillaris

inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by

interferon-gamma Plus LPS." Arch Pharm Res. 2005; 28(2):203-8 (ISSN: 0253-6269) Another group of scientists wrote "The hepatoprotective activity of crude extract of artemisia scoparia (aerial parts) was investigated against experimentally produced hepatic damage using carbon tetrachloride (CCl4) as a model hepatotoxin. CCl4 at the dose of 1.5 ml/kg, produced liver damage in rats as manifested by the rise in serum levels of AST and ALT to 395 +/- 110 and 258 +/- 61 IU/l (mean +/- SEM; n = 10) respectively, compared to control values of 106 +/- 15 and 26 +/- 04. Pretreatment of rats with plant extract (150 mg/kg) significantly lowered (P < 0.01), the respective serum GOT and GPT levels to 93 +/- 05 and 27 +/- 03 IU/l, indicating hepatoprotective action. Pentobarbital sodium (75 mg/kg)-induced sleeping time in mice was found to be 140.8 +/- 1.5 min (n = 10) which was similar (P > 0.05) to that obtained in the group of animals pretreated with the plant extract (139.9 +/- 1.8 min). CCl4 treatment extended the pentobarbital sleeping time to 212.2 +/- 19.1 min and pretreatment of animals with plant extract reversed the CCl4-induced prolongation in pentobarbital sleeping time to 143.9 +/- 5.5 min (P < 0.001) which further confirms the protective action of the plant extract against CCl4-induced liver damage. These data indicate that the plant artemisia scoparia is hepatoprotective and validate the folkloric use of this plant in liver damage". "Hepatoprotective effects of artemisia scoparia against carbon tetrachloride: an environmental

contaminant." J Pak Med Assoc. 1994 Mar;44(3):65-8. "Tissue factor (TF) is an important regulator and effector molecule of coagulation in various inflammatory states. In sepsis, expression of TF by activated endothelial cells leads to disseminated intravascular coagulation. Scoparone is extracted from the traditional Chinese herb Artemisia

scoparia and is known to have potent anti-inflammatory properties. In the current studies, we examined the effects of scoparone on inhibiting lipopolysaccharide (LPS)-induced TF expression in cultured human umbilical vein endothelial cells (HUVECs). Flow-cytometric analysis revealed LPS (10 $g/ml)-activated surface TF induction was concentration-dependently inhibited by scoparone (10-400 $M). The concentrations of scoparone used in this study did not affect cell viability. The elevation of the procoagulant activity of TF by LPS was suppressed by scoparone. The LPS-induced superoxide formation was markedly decreased by scoparone. Messenger RNA expression of TF in LPS-activated HUVECs was also reduced by scoparone. Furthermore, scoparone did not significantly affect the IB degradation. Our results demonstrate that the inhibition of scoparone on LPS-induced TF expression in HUVECs may mediate by the mechanisms suppressing superoxide anion formation and TF transcription." "Scoparone Inhibits Tissue Factor Expression in Lipopolysaccharide-

Activated Human Umbilical Vein Endothelial Cells." Journal of Biomedical Science

2003;10:518-525 (DOI: 10.1159/000072379) "A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris Thunb

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(Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 microg/mL) inhibited the secretion of EtOH-induced interluekin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). AC also inhibited the EtOH-, IL-1alpha-, and TNF-alpha-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells." "Inhibitory effect of Artemisia capillaris on ethanol-induced

cytokines (TNF-alpha, IL-1alpha) secretion in Hep G2 cells." Immunopharmacol

Immunotoxicol. 2002 Aug;24(3):441-53. "Rutin, a well-known flavonoid was investigated for its possible protective effect against paracetamol- and CCl(4)-induced hepatic damage. Paracetamol produced 100% mortality at the dose of 1 g/kg in mice while pre-treatment of animals with rutin (20 mg/kg) reduced the death rate to 40%. Oral administration of a sub-lethal dose of paracetamol (640 mg/kg) produced liver damage in rats as manifested by the rise in serum level of transaminases (AST and ALT). Pre-treatment of rats with rutin (20 mg/kg) prevented the paracetamol-induced rise in serum enzymes. The hepatotoxic dose of CCl(4) (1.5 ml/kg; orally) also raised the serum AST and ALT levels. The same dose of rutin (20 mg/kg) was able to prevent the CCl(4)-induced rise in serum enzymes. Rutin also prevented the CCl(4)-induced prolongation in pentobarbital sleeping time confirming its hepatoprotectivity. These results indicate that rutin possesses hepatoprotective activity and the presence of this compound in Artemisia scoparia may explain the folkloric use of the plant in liver damage." "Protective effect of

rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents." Fitoterapia. 2002 Dec;73(7-

8):557-63. "Hepatoprotective activity of hydro-methanolic extract of Artemisia scoparia (Compositae) was investigated against acetaminophen-induced hepatic damage. 2. Acetaminophen at a dose of 1 g/kg produced 100% mortality in mice while pretreatment of animals with plant extract (150 mg/kg) reduced the death rate to 20%. 3. Acetaminophen at a dose of 640 mg/kg produced liver damage in rats as manifested by the rise in serum levels of GOT and GPT to 1528 +/- 310 and 904 +/- 261 IU/l (n = 10) respectively, compared to respective control values of 80 +/- 11 and 38 +/- 09. 4. Pretreatment of rats with plant extract (150 mg/kg) lowered significantly the respective serum GOT and GPT levels to 85 +/- 11 and 23 +/- 06. 5. These results indicate that Artemisia scoparia contains hepatoprotective constituents and this study rationalizes the traditional use of this plant in hepatobiliary disorders." "Protective effect of Artemisia scoparia extract against acetaminophen-

induced hepatotoxicity." Gen Pharmacol. 1993 Nov;24(6):1455-8. 5. Radix Glycyrrhiza Glabra

Function of the herb: • Cleanse and protect the liver • Promotes production of red blood cells • Invigorates the function of the heart and the spleen • Improves the vital energy • Improves the immune system

"Aflatoxin B(1) (AFB(1)) causes oxidative stress and ROS formation via metabolic activation of AFB(1). Glycyrrhizic acid (GA) has been reported to have antioxidative properties. The present study was to investigate the effect of GA, a major component of licorice on AFB(1)-induced cytotoxicity in human hepatoma cell line (HepG2). GA displayed protective effects against AFB(1) treatment. Both CYP1A1, and glutathione S-transferase (GST) activities were increased in cells after treatment with the GA, while CYP1A2 did not seem to be affected by GA. For cells without GA pre-treatment, cell injury was implicated as indicated by the decrease in cell viability. The time-course study of GA showed pretreatment of cells with GA for 24 h was effective. The treatment of cells with GA and AFB(1) enhanced the detoxifying enzyme activity. The pre-treatment of cells with GA provides protective effects in terms of the enzyme activity and increase in cell viability. The results suggest that GA protects against aflatoxin-induced oxidative stress. This may contribute to its anticarcinogenic

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capability. The protective effect is likely due to its capacity to inhibit the metabolic activation of hepato-toxin, a critical factor in the pathogenesis of chemical-induced carcinogenicity." "Inhibition

of glycyrrhizic acid on aflatoxin B1-induced cytotoxicity in hepatoma cells" Toxicology. 2003

Jun 30;188(2-3):211-7". "Isoflavan derivatives, glabridin (1), hispaglabridin A (2), hispaglabridin B (3), 4'-Omethylglabridin (4) and 3'-hydroxy-4'-O-methylglabridin (5), isolated from Glycyrrhiza glabra, were investigated for their ability to protect liver mitochondria against oxidative stresses. Mitochondrial lipid peroxidation linked to respiratory electron transport and that induced non-enzymatically were inhibited by these isoflavans. Hispaglabridin A (2) strongly inhibited both peroxidations and 3'-hydroxy-4'-O-methylglabridin (5) was the most effective at preventing NADH-dependent peroxidation. 3'-Hydroxy-4'-O-methylglabridin (5) protected mitochondrial respiratory enzyme activities against NADPH-dependent peroxidation injury. Dihydroxyfumarate-induced mitochondrial peroxidation was also prevented by this isoflavan. Isoflavans from G. glabra were shown to be effective in protecting mitochondrial function against oxidative stresses." "Protection of mitochondrial functions against

oxidative stresses by isoflavans from Glycyrrhiza glabra." J Pharm Pharmacol. 2000

Feb;52(2):219-23. "Glycyrrhizin, an aqueous extract of licorice root, has anti-inflammatory activity and has been used for the treatment of chronic viral hepatitis. In the present study we describe the mechanism by which glycyrrhizin inhibits complement. Glycyrrhizin inhibited the cytolytic activity of complement via the activation of both the classical and alternative pathways, while it had no effect on immune adherence, suggesting that it blocks C5 or a later stage of the complement cascade. Further analysis revealed that glycyrrhizin inhibits the lytic pathway in which the membrane attack complex (MAC) is formed. This mechanism suggests that glycyrrhizin may prevent tissue injury caused by MAC not only in chronic hepatitis but in many autoimmune and inflammatory diseases." "Glycyrrhizin inhibits the lytic

pathway of complement--possible mechanism of its anti-inflammatory effect on liver cells in

viral hepatitis." Microbiol Immunol. 2000;44(9):799-804 "(R)-4-(3,4-Dihydro-8,8-dimethyl)-2H,8H-benzo[1,2-b:3,4-b']dipyran-3yl)-1,3-benzenediol

(glabridin), a flavonoid present in licorice extract, is known to have antimicrobial, anti-inflammatory, and cardiovascular protective activities. In the present study, we report the inhibitory effect of glabridin on nitric oxide (NO) production and inducible nitric oxide (iNOS) gene expression in murine macrophages. Glabridin attenuated lipopolysaccharide (LPS)-induced NO production in isolated mouse peritoneal macrophages and RAW 264.7 cells, a mouse macrophage-like cell line. Moreover, iNOS mRNA expression was also blocked by glabridin treatment in LPS-stimulated RAW 264.7 cells. Further study demonstrated that the LPS-induced nuclear factor (NF)- B/Rel DNA binding activity and NF- B/Rel-dependent reporter gene activity were significantly inhibited by glabridin in RAW 264.7 cells and that this effect was mediated through the inhibition of inhibitory factor- B degradation and p65 nuclear translocation. Moreover, reactive oxygen species generation was also suppressed by glabridin treatment in RAW 264.7 cells. In contrast, the activity of mitogen-activated protein kinases

was unaffected by glabridin treatment. In animal model, in vivo administration of glabridin increased the rate of survival of LPS-treated mice and inhibited LPS-induced increase in plasma concentrations of nitrite/nitrate and tumor necrosis factor- . Collectively, these data suggest that glabridin inhibits NO production and iNOS gene expression by blocking NF- B/Rel activation and that this effect was mediated, at least in part, by inhibiting reactive oxygen species generation. Furthermore, in vivo anti-inflammatory effect of glabridin suggests a possible therapeutic application of this agent in inflammatory diseases." "Glabridin, an Isoflavan from Licorice Root, Inhibits Inducible Nitric-

Oxide Synthase Expression and Improves Survival of Mice in Experimental Model of Septic

Shock." JPET 312:1187-1194, 2005


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4) Breath-Ezy


1. Weak lungs 2. Excessive sweating 3. Expectorant for asthma 4. Improves immune system 5. Chronic bronchitis 6. Bronchial asthma 7. Pulmonary emphysema (improves lung function) 8. Improves intellectual activity and concentration 9. Improves endurance 10. Prevents loss of energy

Herbs:

Perilla Frutescens Semen Sinapis Alba Radix Pinella Ternata Cinnamoni Ramulus Cinnamomum Cassia Zingiberis Rz Glycyrrhiza Rx

1. Perilla Frutescens

Function of the herb: • Used as anti-asthmatic herb • Expectorant for chronic bronchitis

Chronic systemic inflammation can inflict degenerative effects throughout the body. A primary cause of this destructive cascade is the production of cell-signaling chemicals known as inflammatory cytokines. Along with these dangerous cytokines, imbalances of hormone-like messengers called prostaglandins also contribute to chronic inflammatory processes. The body needs fatty acids to survive and is able to make all but two of them: linoleic acid, in the omega-6 family and linolenic acid, in the omega-3 family. These two fatty acids must be supplied by the diet and are therefore considered essential fatty acids (EFAs). Perilla is rich in linolenic/alpha-linolenic acid (ALA). Since alpha-linolenic acid or ALA cannot be manufactured by the body it must be obtained from food. ALA, as well as the fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), belongs to a group of fatty acids called omega-3 fatty acids. EPA and DHA are found primarily in fish while ALA is highly concentrated in plants like perilla. Once ingested, the body converts ALA to EPA and DHA, the two types of omega-3 fatty acids more readily used by the body. If we can consume sufficient ALA then intake of EPA and DHA is optional. Omega-3 fatty acids help reduce inflammation and most omega-6 fatty acids tend to promote inflammation. Omega-3 fatty acids have been shown to reduce inflammation and help prevent certain chronic diseases such as lung disease like asthma and arthritis. These essential fatty acids are highly concentrated in the brain and appear to be particularly important for cognitive

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"The leaves of Perilla frutescens Britton (Labiatae) are one of the most popular garnishes in Japan, used as an antidote for fish and crab meat allergy or as a food colorant. The present study was conducted to evaluate its anti-allergic effect and to identify its active constituents using mice ear-passive cutaneous anaphylaxis (PCA)-reaction. 48 hr after the cutaneous injection of anti-ovalbumin serum into the ears of mice, ovalbumin and evansblue dye were intravenously injected. Perilla was extracted with boiling water, and intraperitoneally injected 15 min before ovalbumin-treatment. Thirty min after ovalbumin-treatment, the ears were removed and the colorant in the ear was colorimetrically quantitated. Perilla extract significantly suppressed the PCA-reaction, which was brought about by rosmarinic acid with a partial contribution from some macromolecular compounds. The anti-allergic titer of rosmarinic acid was more effective than tranilast, which is a modern anti-allergic drug. Perilla and rosmarinic acid are potentially promising agents for the treatment of allergic diseases.". "Anti-


Mar;17(3):240-3 Okamato M, et al.Dept of Medicine, Misasa Medical Branch, Tottori, Japan. Dietary sources of alpha-linolenic acid, such as perilla seed oil, may have the capacity to inhibit the generation of leukotrienes by leucocytes in patients with asthma, as has been reported with the consumption of other long-chain n-3 fatty acids. The factors affecting the suppression of leukotriene (LT) C4 generation by leucocytes were examined by comparing the clinical features of patients with asthma who had been given dietary perilla seed oil (n-3 fatty acids). Group A consisted of patients in whom the leucocytes generation of LTC4 was suppressed by dietary perilla seed oil. Group B consisted of those in whom LTC4 generation was not suppressed. Conclusions: Dietary supplementation with perilla seed oil in selected patients with asthma suppresses the generation of LTC4 and is associated with clinical features such as respiratory function and lipometabolism. “Effects of perilla seed oil supplementation on


Int Arch Allergy Immunol 2000 Jun; 122 (2) 137-42. Okamoto M. et al., Okayama University Medical School, Tohaku-gun, Tottori. The effects of perilla seed oil (n-3fatty acids) on bronchial asthma were compared with the effects of corn-oil (n-6 fatty acids) in relation to the pulmonary function and the generation of Leukotriene b4 (LTB4) and C4 (LTC4) by leucocytes. 14 asthmatics subjects were divided randomly into two groups: one group of 7 consumed perilla seed oil-rich supplementation and the other group of 7 consumed corn-oil rich supplementation for 4 weeks. Conclusion: The results suggest that perilla seed-oil-rich supplementation is useful for the treatment of asthma in terms of suppression of LTB4 and LTC4 generation by leucocytes, and improvement of pulmonary function. “Effects of dietary


Intern Med 2000 Feb; 39(2):107-11.

2. Semen Sinapis Alba


• Used for cough • For stuffy feeling in the chest • Warm the lung, promote energy circulation and eliminate phlegm

"Sinapis alba is effective for strengthening the lungs. The effect of naturally occurring allyl and phenyl isothiocyanates found in this herb in the inhibition of B16F-10 melanoma cells-induced metastasis has been studied in C57BL/6 mice. The two compounds were administered by three different modalities. Simultaneous administration of the compounds with tumour induction was found to produce significant reduction in the lung tumour nodule formation; of the two compounds studied, allyl isothiocyanate have shown to be more effective and it produced 93% inhibition of tumour nodules while phenyl isothiocyanate has an inhibition of 77.3%. Prophylactic administration also produces considerable effect but no effect was observed by their administration after the tumour development. Lung collagen hydroxyproline, lung uronic acid, lung hexosamine content, serum sialic

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acid and serum GGT levels were also estimated and the enhanced levels of these parameters in the control animals were found to be reduced by the simultaneous administration of these isothiocyanates; these data were also correlated with the inhibition of lung tumour nodules produced and the increase in life span of the animals." "Effect of naturally occurring allyl and phenyl isothiocyanates in the

inhibition of experimental pulmonary metastasis induced by B16F-10 melanoma cells.

Fitoterapia. 2003 Jun;74(4):355-63

"Peptide mass fingerprinting of tryptic peptides of the major polypeptides by mass spectrometry indicated that the calcium-soluble protein isolate (CSPI) is composed mainly of cruciferin proteins with a contribution from napins (the major allergenic proteins of S. alba). The S. alba CSPI had significantly higher (p < 0.05) protein solubility and emulsion formation ability in the presence of 0.75 M calcium chloride when compared to similar isolates prepared from Brassica juncea (brown mustard) and soybean seed meals. We suggest that the S. alba CSPI could be used to prepare calcium-fortified high protein liquid products." "Characterization of a calcium-soluble protein fraction

from yellow mustard (Sinapis alba) seed meal with potential application." J Agric Food Chem.

2004 Sep 22;52(19):6030-4

3. Radix Pinella Ternata

Function of the herb: • Effective as an expectorant • Used for asthma problems

4.Cinnamoni Ramulus

Function of the herb: • Effective as an expectorant • Used for asthma problems

5. Cinnamomum Cassia

Function of the herb: • To warm up vital function of kidney

6. Zingiberis Rz

Function of the herb: • To warm up the lung for chronic bronchitis with thin, white and foamy phlegm

7. Glycyrrhiza Rx

Function of the herb: • It is used to invigorate functions of the heart and spleen for the treatment of symptoms due to

deficiency of vital energy of these viscera. Licorice has long been used to treat a variety of inflammatory conditions such as asthma, skin diseases (e.g., psoriasis or eczema), peptic ulcers, and even rheumatoid arthritis. Although for the most part good clinical studies in this area are lacking, the existing evidence for the anti-inflammatory effects of particularly GA at least support the therapeutic rationale for some of these treatments. Early reports from the late 1950s demonstrated that 2% topical preparations of GA were beneficial in inflammatory cutaneous disorders.40 Animal studies utilizing rodent models of inflammation during this period confirmed the efficacy of these clinical findings.41 More recently, Inoue et al. demonstrated the potent anti-inflammatory effect of GA on TPA-induced mouse ear edema.42 Both oral and topical preparations of GA when given 30 min before TPA treatment inhibited edema formation. A possible mechanism for these anti-inflammatory effects was offered by Shiki et al., who showed that GL stabilizes lysosomes by inhibiting phospholipase A activity in the lysosomal

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membrane.43 This prevented the release of proteolytic enzymes and acid phosphatases from lysosomes into damaged tissue. Another possible anti-inflammatory mechanism could involve GA’s ability to prevent mononuclear leucocyte migration into the injured tissue as shown in a carrageenan-induced rat paw edema model.44 An additional mechanism could involve GA inhibition of the human complement cascade as shown by Kroes et al.45 Anti-inflammatory Effects: The above-mentioned anti-inflammatory effects of GA and GL resemble cortisol’s effect on the inflammation process. Recent studies show that GA is a potent inhibitor of the skin enzyme 11-b-hydroxysteroid dehydrogenase (11-?OHSD) which catalyses the conversion of active cortisol into less active metabolites.46 Thus, GA likely acts by potentiating the known anti-inflammatory effects of cortisol. This idea is further supported by studies showing that GA, GL and carbenoxolone do not have anti-inflammatory effects in adrenalectomized animals, indicating that the effects are due to cortisol. This inhibition of 11-?OHSD appears to occur in most all tissues. In rats this enzyme activity was decreased in most organs after intraperitoneal injection of GA.47

Antitussive and Expectorant Effects: Licorice has through the years developed a reputation as an effective expectorant and cough suppressant chiefly used to treat the symptoms of the common cold. Licorice extracts are often found in a variety of throat lozenges. However, no clinical studies have been carried out supporting this widespread use. The cough suppressant effect could be related to its demulcent properties on irritated throat tissues. The well-documented protective effects on gastric mucosal cells could also occur in the upper esophagus and throat, perhaps explaining some of its claimed antitussive effects. The strong, sweet taste of GL could be partly responsible for its expectorant properties. 40. Colin-Jones E. Glycyrrhetinic acid. Br Med J. 1957;161. 41. Finney R, Somers G. The anti-inflammatory activity of glycyrrhetinic acid and derivatives. J Pharm Pharmacol. 1958;10:613-620. 42. Inoue H, Mori T. Modulation by glycyrrhetinic acid derivatives of TPA-induced mouse ear oedema. B J Pharmacol. 1989;96:204-210. 43. Shiki Y, Ishikawa Y, et al. Effect of glycyrrhizin on lysosome labilization by phospholipase A2. Amer J Chin Med.1986;14 (3-4):131-137. 44. Capasso F, Mascoto N, et al. Glycyrrhetinic acid, leucocytes and prostaglandins. J Pharm Pharmacol. 1983;35:332-335. 45. Kroes B, Beukelman C, et al. Inhibition of human complement by b-glycyrrhetinic acid. Immunology. 1997;90(1):115-120. 46. Teelucksingh S, Mackie A, et al. Potentiation of hydrocortisone activity in skin by glycyrrhetinic acid. Lancet. 1990:1060-1063. 47. Marandici A, Monder C, et al. Inhibition by glycyrrhetinic acid of rat tissue 11-hydroxysteroid dehydrogenase in vivo. Steroids. 1993;58(4):153-156. "(R)-4-(3,4-Dihydro-8,8-dimethyl)-2H,8H-benzo[1,2-b:3,4-b']dipyran-3yl)-1,3-benzenediol

(glabridin), a flavonoid present in licorice extract, is known to have antimicrobial, anti-inflammatory, and cardiovascular protective activities. In the present study, we report the inhibitory effect of glabridin on nitric oxide (NO) production and inducible nitric oxide (iNOS) gene expression in murine macrophages. Glabridin attenuated lipopolysaccharide (LPS)-induced NO production in isolated mouse peritoneal macrophages and RAW 264.7 cells, a mouse macrophage-like cell line. Moreover, iNOS mRNA expression was also blocked by glabridin treatment in LPS-stimulated RAW 264.7 cells. Further study demonstrated that the LPS-induced nuclear factor (NF)-B/Rel DNA binding activity and NF- B/Rel-dependent reporter gene activity were significantly inhibited by glabridin in RAW 264.7 cells and that this effect was mediated through the inhibition of inhibitory factor- B degradation and p65 nuclear translocation. Moreover, reactive oxygen species generation was also suppressed by glabridin treatment in RAW 264.7 cells. In contrast, the activity of mitogen-activated protein kinases

was unaffected by glabridin treatment. In animal model, in vivo administration of glabridin increased the rate of survival of LPS-treated mice and inhibited LPS-induced increase in plasma concentrations of nitrite/nitrate and tumor necrosis factor- . Collectively, these data suggest that glabridin inhibits NO production and iNOS gene expression by blocking NF- B/Rel activation and that this effect was mediated, at least in part, by inhibiting reactive oxygen species generation. Furthermore, in vivo anti-inflammatory effect of glabridin suggests a possible therapeutic application of this agent in inflammatory diseases." "Glabridin, an Isoflavan from Licorice Root, Inhibits Inducible Nitric-Oxide

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Synthase Expression and Improves Survival of Mice in Experimental Model of Septic Shock" Journal of Pharmacology And Experimental Therapeutics Fast Forward First published on November 10, 2004; DOI: 10.1124/jpet.104.077107 For testimonies kindly go to this webpage for more information:

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5) ColonEase


1. Colon Cleanse 2. Constipation (taken together with 40 Plus Tonic and Spitalcare)

Herbs:

Rheum Palmatum Herba Lysimachia Christinae Poria Cocos Wolf Polyporus Umbellatus Caulis Uncaria Rhynchophylla

1) Radix Rheum Palmatum

Function of the herb: • Relieves pain in case of injury or inflammation • It is used for detoxification

Cell adhesion and spreading is a crucial step in the metastatic cascade of cancer cells, and interruption of this step is considered to be a logical strategy for prevention and treatment of tumor metastasis. Emodin is the major active component of the rhizome of Rheum palmatum L., with known anticancer activities. Here, we first found that emodin significantly inhibited cell adhesion of various human cancer cells. This inhibition was achieved through suppressing the recruitment of focal adhesion kinase (FAK) to integrin ß1 as well as the phosphorylation of FAK followed by the decreased formation of focal adhesion complex (FAC). In understanding the underlying mechanisms, we found that emodin inhibited the lipid raft clustering and subsequent colocalization of integrin ß1 and FAC proteins within lipid rafts. Lipid profile analysis revealed significant decrease of cholesterol and sphingolipids in raft fraction after emodin treatment. Cholesterol replenishment abolished the adverse effect of emodin on the translocation of integrin ß1 and FAC proteins into the lipid raft fraction and cell adhesion. Therefore, data from this study provide novel evidence that emodin inhibits cell adhesion and spreading through disruption of the membrane lipid raft-associated integrin signaling pathway. (Cancer Res 2006; 66(11): 5807-15)

"Emodin Inhibits Tumor Cell Adhesion through

Disruption of the Membrane Lipid Raft-Associated Integrin Signaling Pathway " [Cancer

Research 66, 5807-5815, June 1, 2006] © 2006 American Association for Cancer Research 2) Herba Lysimachia Christinae

Function of the herb: • Counteract toxic effects • To clear up liver heat • Expel urinary stones and promote urination

3) Poria Cocos Wolf


• It has regulatory effect on human immune cells

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"Because of the reported immune-enhancing and anti-tumor activities of some mushroom polysaccharides, their applications as biological response modifiers have attracted significant attention. We have purified a water-soluble beta-glucan PCM3-II, comprising mainly 1right curved arrow 3 and 1right curved arrow 4 linkages, from the mycelia of Poria cocos (Schw.) Wolf (Fu-ling). In this study, the growth-inhibitory effect of PCM3-II was further explored on the human breast carcinoma MCF-7 cells in vitro. The dose effect of PCM3-II was studied by incubating the breast cancer cells with 12.5-400 microg/ml of the glucan for 72 h. The MTT study showed that PCM3-II reduced proliferation and viability of the MCF-7 cells dose-dependently, so that the cancer-cell growth was decreased by 50% of the control level at 400 microg/ml of the glucan. The time effect of PCM3-II was then investigated by treating the breast cancer cells with 400 microg/ml of the glucan for 24, 48 and 72 h, respectively. Results from the flow cytometry study demonstrated that PCM3-II induced cell-cycle G1 arrest time-dependently and about 90% of the cells in cell cycle were accumulated at G1 phase after 72 h of treatment. The G1 arrest was associated with downregulations of the unscheduled cyclin D1 and cyclin E expressions in the breast cancer cells. Apoptosis was also induced by PCM3-II in the MCF-7 cells, so that the subG1 cells in DNA histogram of the flow cytometry were elevated by 5-fold of the control level at 48 h and by 24-fold at 72 h of treatment. The immunoblot study also showed that the glucan induced depletion of the antiapoptotic Bcl-2 protein, but not the proapoptotic Bax protein, so that the Bax/Bcl-2 ratio was elevated in the breast cancer cells at the time when the most prominent apoptosis was also observed. In conclusion, although the detailed mechanism for the anti-tumor activity of the P. cocos beta-glucan still needs further investigation, this study provides preliminary insights into its mode of action and perspectives of its development as a water-soluble anti-tumor agent." "Growth-inhibitory effects of a beta-glucan

from the mycelium of Poria cocos on human breast carcinoma MCF-7 cells: cell-cycle arrest

and apoptosis induction." Oncol Rep. 2006; 15(3):637-43 (ISSN: 1021-335X) 4) Polyporus Umbellatus

Function of the herb: • It is used for detoxification • It is used as a diuretic

Below are some research on the herb.

"Grifola umbellata (also known as Polyporus umbellatus) is a mushroom that is used as a diuretic in Chinese medicine. Herein, we report on the isolation and identification of a cytotoxic compound from the sclerotia of G. umbellata. For the isolation of the compound, a hexane soluble fraction of the sclerotia of G. umbellata was subjected to column chromatography on silica gel and/or Sephadex LH-20 column eluted with organic solvents. The structure of the isolated compound was elucidated using IR, MS, 1H-, and 13C-NMR spectra, and the structure of the compound was determined as ergosta-4, 6, 8 (14), 22-tetraen-3-one (erogone). Cytotoxic activities of erogone compared to human cancer cell lines, HT-29 (colon cancer), HeLa 229 (cervix cancer), Hep3B (liver cancer), and AGS (stomach cancer) were compared using the XTT assay kit. Ergone inhibited all cell lines as the dose was increased. In the case of Hep3B and HT-29 cell lines, maximal cytotoxic activities of erogone were achieved at the concentrations of 10 and 15 µ/mL, respectively. However, the cytotoxic activities of erogone compared to HeLa 229 and AGS were much weaker than those of Hep3B and HT-29 cell lines. Values of 50% inhibitory concentrations (IC50) of erogone against Hep3B, HT-29, HeLa 229, and AGS were 5, 7.2, 26.3, and 22 µ/mL, respectively. Cytotoxic activities of erogone against various tumor cell lines were evaluated in this study for the first time. Therefore, other mushrooms were also examined for the presence of the compound. In the present study, we measured the content of erogone in eight mushrooms—Grifola umbellata, Lentinus edodes (Berk.) Singer, Ganoderma applanatum (Pers.:Wallr.) Pat., Tricholoma matsutake (S.Ito et S.Imai) Singer, Sarcodon aspratus (Berk.) S.Ito, Ramaria botrytis (Pers.) Ricken, Pleurotus eryngii (DC.:Fr.) Quél., and Sparassis crispa (Wulf.)Fr.—and four mycelia—Grifola umbellata, Lentinus edodes, Ganoderma applanatum, and Tricholoma

matsutake—using HPLC. The contents of erogone in mushroom and mycelium were in the range of

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4.8%29.0 µ/g and 15.5%38.0 µ/g, respectively. Among mushrooms and mycelia tested, the mycelia of Grifola umbellata and Tricholoma matsutake had the highest amounts of erogone (38.0 µ/g)." "Cytotoxic Activities of Ergosta-4, 6, 8 (14), 22-tetraen-3-one from the Sclerotia of Grifola

umbellata" International Journal of Medicinal Mushrooms ISSN 1521-9437 Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats. "Inhibitory effects of triterpenes isolated from Chuling

(Polyporus umbellatus Fries) on free radical-induced lysis of red blood cells." Biol Pharm Bull.

2005; 28(5):817-21 (ISSN: 0918-6158) In another research by the Medical Department of Tokyo University, the National Cancer Center Laboratory and Tokyo College of Pharmacy of the herb on guinea pigs show a significant effect on the anti-cancer activity of the main compound beta (1-3)(1-6) D-Glucans. 5) Caulis Uncaria Rhynchophylla

Function of the herb: • It is used as antipyretic and anticonvulsive agent

Uncaria rhynchophylla (Miq.) Jack (UR) and Gastrodia elata BI. (GE) are traditional Chinese herbs that are usually used in combination to treat convulsive disorders, such as epilepsy, in China. The aim of this study was to compare the anticonvulsive and free radical scavenging activities of UR alone and UR in combination with GE in rats. For the in vitro studies, brain tissues from 6 male Sprague-Dawley (SD) rats were treated with 120 microg/ml kainic acid (KA), with or without varied concentrations of UR or UR plus GE. For the in vivo studies, male SD rats (6 per group) received intraperitoneal (i.p.) injection of KA 12 mg/kg to induce epileptic seizures and generation of free radicals, with or without oral administration of UR 1 g/kg alone or UR 1 g/kg plus GE 1 g/kg. Epileptic seizures were verified by behavioral observations, and electroencephalography (EEG) and electromyography (EMG) recordings. These results showed that UR alone decreased KA-induced lipid peroxide levels in vitro, whereas UR plus GE did not produce a greater effect than UR alone. UR significantly reduced counts of wet dog shakes (WDS), paw tremor (PT) and facial myoclonia (FM) in KA-treated rats and significantly delayed the onset time of WDS, from 27 min in the control group to 40 min in the UR group. UR plus GE did not inhibit seizures more effectively than UR alone, but did further prolong the onset time of WDS to 63 min (P < 0.05 vs. UR alone). UR alone reduced the levels of free radicals in vivo, as measured by lipid peroxidation in the brain and luminol-chemiluminescence (CL) counts and lucigenin-CL counts in the peripheral whole blood, but the combination of GE and UR did not reduce free radical levels more markedly than UR alone. In conclusion, our results indicate that UR has anticonvulsive and free radical scavenging activities, and UR combined with GE exhibit greater inhibition on the onset time of WDS than UR alone. These findings suggest that the anticonvulsive effects of UR and GE may be synergistic. However, the mechanism of interaction between UR and GE remains unknown. "Anticonvulsive and free radical scavenging actions of two herbs, Uncaria rhynchophylla (MIQ) Jack and Gastrodia elata Bl., in kainic acid-treated rats." Life Sci (1999) 65: 2071-82 For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/Intestcleanse_Testimonials.htm

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6) D-Be-Te

1. Diabetes • Lowers blood sugar • Treats Retinopathy (eye problems) • Treats Neuropathy • Improves the function of liver, spleen, kidney, pancreas and bladder

Herbs:

Radix Rehmannia Glutinosa Radix Trichosanthis Semen Cuscutae Radix Polygonum Multiflorum Herba Cynomorii Semen Plantaginis Poria Cocos Wolf

Research: A) Research has shown that Radix Rehmannia Glutinosa , Radix Trichosanthis, Radix Polygonum Multiflorum and Poria Cocos Wolf are effective in treating diabetes and its related problems. I) Hsu Hong – Yen and Hsu Chau – Shin, “Commonly Used Chinese Herb Formulas with

Illustrations”, Oriental Healing Arts Institute 1980. II) Hsu Hong Yen and Kuwaki Takahide, “Diabetes Mellitus and Chinese Herb Formulas

Bulletin of the Oriental Healing Arts Institute Vol. 3, No 1, p 13 1978. III) Chen D.C., Gong D. Q., and Zhai Y, Journal of Traditional Chinese Medicine 14 (3): 163

– 166, 1994. IV) Lin L et al., “A Clinical Study on Treatment of Vascular Complications of Diabetes”,

Journal of Chinese Medicine 14 (1): 3 – 9, 1994. B) Research from The School of Pharmacy, Shanghai Jiao Tong University, Shanghai China, The College of Pharmaceutical Science and Technology, Tianjin University, Tianjin China and Tianjin Institute of Pharmaceutical Research, Tianjin China shows that polysaccharide of Poria Cocos Wolf and Radix Rehmannia Glutinosa can restore the functions of pancreatic tissues and can cause an increase in insulin output by the functional beta cells, while other ingredients of these plants enhance the microcirculation, increasing the availability of insulin and facilitating the metabolism in the insulin dependent process. Pharmacological and clinical evaluation indicated that these herbs can significantly lower blood glucose. C) In a research by Dr. K. Casanas and team, “The Treatment of Diabetes Mellitus with Chinese Medicine” a formulation containing Radix Rehmannia Glutinosa, Radix Trichosanthis and other herbs was tested on 150 cases of Type 2 Diabetes Mellitus. 138 cases showed great improvement in their diabetic condition. D) Test on animals showed that Semen Cuscutae can improve the function of the body, kidney and thymus gland as well as increasing their white cell count, red cell count, hemoglobin content and superoxide dismutase (SOD) activity. Mi Hemin ed. Comparison Study on the bone support and yang strengthening effects of Semen Cuscutae, Chinese Traditional Herbal Drugs, 1991. 22 (12): 547.

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E) Research has shown that the triterpenoid compounds in Herba Cynomorii (oleanolic acid and ursolic acid) are effective in protecting against chemically induced liver injury in laboratory animals. The researcher suggests that the mechanism of hepatoprotection by these two compounds may involve the inhibition of toxicant activation and the enhancement of the body’s defence system. Oleanolic acid and usolic acid have been long recognized to have anti inflammatory and anti hyperlipidermic properties in laboratory animals. Liu J “Pharmacology of oleanolic acid and ursolic acid” 1995. Dec 1 49 (2): 57 – 68. Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, USA. F) Research has shown that ursolic acid (of Herba Cynomorii) treatment can normalize the disturbed anti-oxidant status of rats intoxicated with CC 14 by maintaining the levels of glutathione and by inhibiting the production of malondiable-hyde due to its radical scavenging properties. Martin-Aragon Si, De Las Heras B etc “Pharmacological modification of endogenous antioxidant enzymes by ursolic acid onTetrachloride induced liver damage in rats and primary cultures of rats hepatocytes”. Exp Toxical. Pathol. 2001:53 (2 – 3) 199 – 206 , Dpto. Farmacologia, UCM, Madrid, Spain. G) Research has shown that the two tripenoid compound, ursolic acid and oleanolic acid can enhance the total white blood cells count of Balb/C mice “Phytomedicine : International Journal of Phytotheraphy and Phytopharmacology”. July 01, 2003, Raphael T.J. and Kuttan G. H) Cucurbitacin are used for diabetes. Dr. S.Dharmananda, Institute for Traditional Medicine, Portland, Oregon. (Cucurbitacin are found in herbs like trichosanthis, citrillus and momordica) I) Plant sterol like those found in Semen Plantaginis has been approved by FDA, USA for the treatment of coronary heart disease. FDA Talk Paper Sept. 5, 2000 J) L-arabinose is a phytochemical of Semen Plantaginis. It is a 5-carbon sugar. Research showed that is has a unique property. It inhibits sucrose activity in the intestine. Sucrose is an enzyme that breaks down sucrose into glucose and fructose. By inhibiting the activity of sucrose, L-arabinose prevents the breakdown of sucrose into glucose and fructose, thus reducing the absorption of sugar into the body. In one rat study, the little critters were fed up to 30 grams of sucrose per 100 gram of feed. Researchers added anywhere between 0 to 1.0 grams of L-arabinose per 100 grams feed. The rats ate this sugar brew for 10 days. Researchers found that lipogenic enzymes (enzymes that build fat) and triglyceride concentrations in the liver went up when the rats consumed sucrose. When L-arabinose was consumed, these changes did not occur. L-arabinose feeding reduced fat weights, plasma insulin and triglycerides. Conclusion: L-arabinose inhibits sucrose activity, reduces sucrose utilization, and decreases lipogenesis and decreases plasma insulin and triglycerides. Ref: Osaki et al “L-arabinose feeding prevents increases due to dietary sucrose in lipogenic enzymes and triacylglycerol levels in rats”. Journal of Nutrition 2001, 131: 796 – 799. K) Researches have shown that plants containing the phyotchemical triterpene (saponin) glycosides have hypoglycemic and hypolipemic activities. The triterpenes compounds are sub divided into 20 groups of which oleanic triterpene (oleanic acid, ursolic acid and alpha-and beta amyrin) is found in the largest variety of plants (Semen Cuscutae, Herba Cynomorii, Radix Rehmannia Glutinosa and Semen Plantaginis). References: 1) Ernst E “Plants with hypoglycemic activity in humans". Phytomedicines 1997; 4 (1): 73-78 2) Marles KJ and Farunworth N.R. “Antidiabetic plants and their active constituents: An update”. Protocal Journal of Botanical Medicine 1996; 1 (3) ; 85-137. 3) Suttisri R, Lee I.S. and Kinghorn D, “Plant-derived triterpenoid sweetness inhibitors”. Journal of Ethnopharmacology 1995; 47:9-26. 4) Hong-Yen Hsu: “Chinese Herb Theology for diabetes mellitus”, Bulletin of the Oriental Healing Acts Institute 1978; 3 (1) : 12-17.

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5) Huang K.L et al., “Studies on the anti hyperglycemic actions of Chinese Herbs”, 1980 – 1984. The Annual Reports of the National Research Institute of Chinese Medicine, Taipei Hsien, Taiwan. 6) Yoshikawa M, Matsuda H. “Anti diabetogenic activity of oleanolic acid glycosides from medicinal foodstuff”. Biofactors 2000: 13: 231-237. For testimonies kindly go to this webpage for more information: http://www.nutrihuang.com/Diabe-X_Testimonials.htm

7) Eve’s Pride


1. Menstrual cramps 2. Irregular menstrual period 3. Sallow (pale) complexion 4. Pale lips and finger nails 5. Tonifies, nourishes and promotes blood flow

Herbs:

Radix Angelica Sinensis Radix Paeonia Lactiflora Radix Atractylodes Lancea Radix Glycyrrhiza Glabra Diospycos Kaki L. 1. Radix Angelica Sinensis (Ought-To-Return, Danggui)


• Invigorate blood circulation • Promote the flow of vital energy for treatment of abnormal menstruation • Regulate menstruation due to hormonal imbalance • Treat headaches and heart disease due to blood stagnation

"Therapeutic effect of Danggui Funing (DGFN) pill in treating 207 patients with abdominal pain were studied with control. Among 207 patients with abdominal pain, 162 of DGFN pill group and 45 of control groups (35 atropine group and 10 placebo group). The effective rate of abdominal pain in the three groups were 93.27%, 97.14% and 0% respectively. There was no significant difference (P > 0.05) between the DGFN pill group and the atropine group, but the difference were statistically significant (P < 0.01) between the above-mentioned two groups and the placebo group. These results revealed that the therapeutic effect of DGFN pill was reliable. There were three pharmacological effects of DGFN pill: The blocking on M, alpha and H1 receptors, the analgesic effect and the antiseptic effect. The DGFN pill was the drug of rapid-efficacy and low toxicity." "Efficacy of

danggui funing pill in treating 162 cases of abdominal pain." Zhongguo Zhong Xi Yi Jie He Za

Zhi. 1992 Sep;12(9):531-2, 517. 2. Radix Paeonia Lactiflora

Function of the herb: • Nourish blood • Regulate menstrual flow • For menstrual cramp • Help ladies' reproductive system

Peony contains a unique glycoside called paeoniflorin. Proanthocyanidins, flavonoids, tannins, polysaccharides, and paeoniflorin are all considered to contribute to the medicinal activity of various forms of peony. Paeoniflorin's major effect seems to be to calm nerves and alleviate spasm. One study

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has confirmed the efficacy of formula with peony and licorice for relieving muscle cramps due to cirrhosis of the liver, diabetes, and dialysis, approved for treatment of muscle cramps. Another formulation combines peony root with dong quai and four other herbs and has been found to effectively reduce symptoms of cramping and pain associated with dysmenorrhea (painful menses). 3. Radix Atractylodes Lancea Function of the herb:

• Expel wind • Relieve pain • Sharpen vision

4. Diospycos Kaki L

Function of the herb: • For treatment of constipation and haemorrhoids • For the treatment of bronchial complaints • For the treatment of hypertension • It is used to treat hiccups • It has anthelmintic, antihaemorrhagic, antivinous, expectorant, febrifuge and restorative

properties 5. Radix Glycyrrhiza Glabra

Function of the herb: • Cleanse and protect the liver • Promotes production of red blood cells • Invigorates the function of the heart and spleen • Improves the vital energy • Improves the immune system • Improves hormonal balances • Prevents osteoporosis and improves bone mass

"Post-menopausal women have higher incidence of heart diseases compared to pre-menopausal women, suggesting a protective role for estrogen. The recently Women's Health Initiative (WHI) randomized controlled trial concluded that the overall heart risk exceeded benefits from use of combined estrogen and progestin as hormone replacement therapy for an average of five years among healthy postmenopausal US women. Therefore, there is an urgent need for new agents with tissue-selective activity with no deleterious effects. In the present study, we tested the effects on vascular tissues in vitro and in vivo of two natural compounds derived from licorice root: glabridin, the major isoflavan, and glabrene, an isoflavene, both demonstrated estrogen-like activities. Similar to estradiol-17beta (E2), glabridin (gla) stimulated DNA synthesis in human endothelial cells (ECV-304; E304) and had a bi-phasic effect on proliferation of human vascular smooth muscle cells (VSMC). Raloxifene inhibited gla as well as E2 activities. In animal studies, both intact females or after ovariectomy, gla similar to E2 stimulated the specific activity of creatine kinase (CK) in aorta (Ao) and in left ventricle of the heart (Lv). Glabrene (glb), on the other hand, had only the stimulatory effect on DNA synthesis in vascular cells, with no inhibition by raloxifene, suggesting a different mechanism of action. To further elucidate the mechanism of action of glb, cells were pre-incubated with glb and then exposed to either E2 or to gla; the DNA stimulation at low doses was unchanged but there was abolishment of the inhibition of VSMC cell proliferation at high doses as well as inhibition of CK stimulation by both E2 and by gla. We conclude that glb behaved differently than E2 or gla, but similarly to raloxifene, being a partial agonist/antagonist of E2. Glabridin, on the other hand, demonstrated only estrogenic activity. Therefore, we suggest the use of glb with or without E2 as a new agent for modulation of vascular injury and atherogenesis for the prevention of cardiovascular diseases in post-menopausal women." "Estrogen-like activity of licorice root constituents:

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glabridin and glabrene, in vascular tissues in vitro and in vivo." J Steroid Biochem Mol Biol.

2004; 91(3):147-55 "Licorice root extract may be helpful for treating symptoms associated with premenstrual syndrome (PMS). Studies have shown that the extract has estrogenic activity and may help to regulate the estrogen-progesterone ratio."

• Costello CH, Lynn EV. Estrogenic Substances from Plants: I. Glycyrrhiza. J Am Pharm Soc 1950; 39: 177-80.

• Kumagai A, Nishino K, Shimomura A, Kin T, Yamamura Y. Effects of glycyrrhizin on estrogen action. Endocrinol Jpn 1967; 14 :34-8.

"A review of the estrogen-like activity of the licorice root derivative glabridin. The effects of this isoflavan, which acts on bone metabolism, were studied in the osteoblastic cell lines of mice. Glabridin significantly increased the growth of these osteoblastic cells and within them caused an elevation of alkaline phosphatase activity, collagen content and osteocalcin secretion. These elevations were prevented by the introduction of cycloheximide and tamoxifen, showing that glabridin’s effects result from a protein component; thus demonstrating its involvement in estrogen actions. A second examination showed that the licorice root derived glabridin prevented the destruction of osteoblastic cells and decreased the production of prostaglandin and nitric oxide in those cells. Therefore, the enhancement of osteoblast function caused by glabridin can help prevent osteoporosis and other inflammatory bone diseases." "The licorice root derived isoflavan glabridin

increases the function of osteoblastic MC3T3-E1 cells" Biochem Pharmacol. 2005 May 24. NIH. "There is an increasing demand for natural compounds that improve women’s health by mimicking the critical benefits of estrogen to the bones and the cardiovascular system but avoiding its deleterious effects on the breast and uterus. The estrogenic properties of glabridin, the major isoflavan in licorice root, were tested in view of the resemblance of its structure and lipophilicity to those of estradiol. The

results indicate that glabridin is a phytoestrogen, binding to the human estrogen receptor and stimulating creatine kinase activity in rat uterus, epiphyseal cartilage, diaphyseal bone, aorta, and left ventricle of the heart. The stimulatory effects of 2.5–25 $g/animal glabridin were similar to those of 5 $g/animal estradiol. Chemical modification of glabridin showed that the position of the hydroxyl

groups has a significant role in binding to the human estrogen receptor and in proliferation-inducing activity. Glabridin was found to be three to four times more active than 2'-O-methylglabridin and 4'-O-methylglabridin, and both derivatives were more active than 2',4'-O-methylglabridin. The effect of increasing concentrations of glabridin on the growth of breast tumor cells was biphasic. Glabridin showed an estrogen receptor-dependent, growth-promoting effect at low concentrations (10 nM–10 $M) and estrogen receptor-independent antiproliferative activity at concentrations of >15 $M. This is the first study to indicate that isoflavans have estrogen-like activities. Glabridin and its derivatives exhibited varying degrees of estrogen receptor agonism in different tests and demonstrated growth-inhibitory actions on breast cancer cells". "Estrogenic and Antiproliferative Properties of

Glabridin from Licorice in Human Breast Cancer Cells" Cancer Research 60, 5704-5709,

October 15, 2000] © 2000 American Association for Cancer Research "Several isoflavans from the licorice root that presented antioxidant activity have been isolated in our lab. Of these, glabridin is the major constituent (11%) of the alcohol extract. Its lipophilicity and its structural similarity to estradiol led us to test it for estrogenic like-activities. In the present study, the properties of newly identified phytoestrogenic compounds, the isoflavans, and isoflavene were investigated by comparing their ability to bind to the human estrogen receptor (ER ) and their effect on estrogen-responsive human breast cancer cells, over a broad range of concentrations. In vivo studies included the effects of glabridin and glabrene on rat uterus wet weight and on the induction of the immediate early “estrogen-induced protein” creatine kinase B in rat skeletal and cardiovascular tissues as well as uterus. Chemical modifications were performed to shed some light on the binding and antiproliferation mechanisms involved. Our results indicate that glabridin and glabrene bound to the human estrogen receptor, exhibited varying degrees of estrogen receptor agonism in vitro and in

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vivo, demonstrated estrogen-independent inhibitory activity on the growth of breast cancer cells and inhibited serotonin re-uptake which may be beneficial as antidepressant agents." Recent Publications

1) Snait Tamir, Mark Eizenberg, Dalia Somjen., Naftali Stern, Rayah Shelach, Alvin Kaye, and Jacob Vaya. Estrogenic and Antiproliferative Properties of Glabridin from Licorice in Human Breast Cancer Cells. Cancer Research. 2000, 60, 5704-5709.

2) Snait Tamir, Mark Eizenberg, Dalia Somjen, Sarit Izrael and Jacob Vaya. Estrogen Like Activity of Glabrene and other Constituents Isolated from Licorice Root. J Steroid Biochem Mol Biol. 2001. 78:291-298. 3) Snait Tamir, Sarit Izrael, Jacob Vaya. The Effect of Oxidative Stress on ERa and ERb Expression. The Journal of Steroid Biochemistry and Molecular Biology (August - 2002), 81 4) Jacob Vaya, Dalia Somjen and Snait Tamir., (2002) Estrogen-Like Activity of Licorice Root Extract and its Constituents. Submitted for publication in: Herbal Medicines: editors; Lester Packer and Barry Halliwell, Publisher- Marcel Dekker For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/Ladycare_Testimonials.htm

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8) InfaLive


1. Poor Appetite (children) 2. Weak Children

Herbs:

Poria Cocos Wolf Agastachis Herba Rhizoma Atractylodis Alba Radix Pueraria Lobata 1. Poria Cocos Wolf

Function of the herb: • It has a regulatory effect on human immune cells

1) Clinical research by western scientists have shown that Poria cocos improves kidney

functions. "The antinephritic (having the effect of reducing or counteracting disease, inflammation, etc in the kidneys) effect of pachyman on original-type anti-GBM nephritis in rats was investigated. Pachyman was given to original-type anti-GBM nephritic rats for 10 days from the day of anti-GBM serum injection. Pachyman prevented urinary protein excretion and the elevation of serum cholesterol content. Histopathological observations of the glomeruli indicated that although the number of nuclei and adhesion to capillary walls of Bowman's capsule in nephritic control rats were significantly increased, pachyman reduced the degree of histopathological changes such as hypercellularity and adhesion as compared to the control group. Although the serum complement CH50 ratio in control group was significantly lower than that in the normal group, the decrease in serum complement CH50 was inhibited by pachyman, and rat C3 deposition in the glomeruli in the pachyman-treated group was significantly reduced. These results suggest that pachyman was effective against original-type anti-GBM nephritis in rats and that the antinephritic mechanisms of pachyman may be partly due to the inhibitory action of this agent on C3 deposition in the glomeruli." "Studies on antinephritic effects

of plant components (3): Effect of pachyman, a main component of Poria cocos Wolf on

original-type anti-GBM nephritis in rats and its mechanisms." Hattori T, Hayashi K, Nagao T,

Furuta K, Ito M, Suzuki Y. Department of Pharmacology, Faculty of Pharmacy, Meijo

University, Nagoya, Japan. Jpn J Pharmacol. 1992 May;59(1):89-96 PMID: 1507662

2) Clinical studies by western scientists have shown that Poria Cocos Wolf has anti-tumor and

immune-enhancing activity. "Because of the reported immune-enhancing and anti-tumor activities of some mushroom polysaccharides, their applications as biological response modifiers have attracted significant attention. We have purified a water-soluble beta-glucan PCM3-II, comprising mainly 1right curved arrow 3 and 1right curved arrow 4 linkages, from the mycelia of Poria cocos (Schw.) Wolf (Fu-ling). In this study, the growth-inhibitory effect of PCM3-II was further explored on the human breast carcinoma MCF-7 cells in vitro. The dose effect of PCM3-II was studied by incubating the breast cancer cells with 12.5-400 microg/ml of the glucan for 72 h. The MTT study showed that PCM3-II reduced proliferation and viability of the MCF-7 cells dose-dependently, so that the cancer-cell growth was decreased by 50% of the control level at 400 microg/ml of the glucan. The time effect of PCM3-II was then investigated by treating the breast cancer cells with 400 microg/ml of the glucan for 24, 48 and 72 h, respectively. Results from the flow cytometry study demonstrated that PCM3-II induced cell-cycle G1 arrest time-dependently and about 90% of the cells in cell cycle were

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accumulated at G1 phase after 72 h of treatment. The G1 arrest was associated with downregulations of the unscheduled cyclin D1 and cyclin E expressions in the breast cancer cells. Apoptosis was also induced by PCM3-II in the MCF-7 cells, so that the subG1 cells in DNA histogram of the flow cytometry were elevated by 5-fold of the control level at 48 h and by 24-fold at 72 h of treatment. The immunoblot study also showed that the glucan induced depletion of the antiapoptotic Bcl-2 protein, but not the proapoptotic Bax protein, so that the Bax/Bcl-2 ratio was elevated in the breast cancer cells at the time when the most prominent apoptosis was also observed. In conclusion, although the detailed mechanism for the anti-tumor activity of the P. cocos beta-glucan still needs further investigation, this study provides preliminary insights into its mode of action and perspectives of its development as a water-soluble anti-tumor agent." Growth-inhibitory effects of a beta-glucan from

the mycelium of Poria cocos on human breast carcinoma MCF-7 cells: cell-cycle arrest and

apoptosis induction." Zhang M; Chiu LC; Cheung PC; Ooi VE Department of Biology, The

Chinese University of Hong Kong, Hong Kong, P.R. China. Oncol Rep. 2006; 15(3):637-43

(ISSN: 1021-335X) PreMedline Identifier: 16465424

2. Herba Agastachis Rugosus

Function of the herb: • Stimulate spleen • Regulate and harmonize stomach function • Improve appetite • Warm middle region • Relieve stomach stagnation

They are used internally to improve the appetite and strengthen the digestive system[238]. Considered to be a "warming" herb, it is used in situations where there is "dampness" within the digestive system, resulting in poor digestion and reduced vitality[254]. [238] Bown. D. Encyclopaedia of Herbs and

their Uses. Dorling Kindersley, London. 1995 ISBN 0-7513-020-31. [254] Chevallier. A. The

Encyclopedia of Medicinal Plants Dorling Kindersley. London 1996 ISBN 9-780751-303148 3. Rhizoma Atractylodis Alba

Function of the herb: • Invigorates function of spleen and stomach • Strengthen spleen • To tone up energy • Protect liver • Dry up dampness • Benefit the lymphatic system • For weakness and spiritlessness

4. Radix Pueraria Lobata

Function of the herb: • It produces body fluid • It benefits the stomach • Elevates energy


http://www.nutrihuang.com/Infalive_Testimonials.htm

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9) NerboneCare

Traditionally used for:

1. Rejuvenates Nerves 2. Rejuvenates Tendons 3. Rejuvenates Ligaments 4. Rejuvenates Bones 5. Rejuvenates Blood Vessels 6. Osteoarthritis Herbs:

Radix Achyranthes Bidentata Semen Prunus Persica Cortex Eucommia Ulmoides Fructus Psoralea Corylifolia Herba Lycopus Lucidus Radix Notopterygium Incisum

1. Radix Achyranthes Bidentatae

Function of the herb: • Nourish liver, kidney and strengthen sinew and bones • Treats aching backs and knees • Treats asthenia (loss of strength/debility) of lower limbs • Regulates and activates blood • Tones up liver and kidneys

Bioassay-directed fractionation of a butanol-soluble fraction of methanol extract of the root of Achyranthes bidentata resulted in the isolation of 5 new oleanolic acid glycosides 1-5, namely, 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester (1), achyranthoside C dimethyl ester (2), achyranthoside C butyl dimethyl ester (3), achyranthoside E dimethyl ester (4), and achyranthoside E butyl methyl ester (5), together with 10 known compounds. Their structures were established on the basis of spectroscopic interpretation and chemical methods. All the oleanolic acid glycosides inhibited the formation of osteoclast-like multinucleated cells (OCLs) induced by 1alpha,25(OH)2D3 in a co-culture assay system. (An osteoclast (from the Greek words for "bone" and "broken") is a type of bone cell that removes bone tissue by removing the bone's mineralized matrix. This process is known as bone resorption. Osteoclasts and osteoblasts are instrumental in controlling the amount of bone tissue. Osteoblasts form bone; osteoclasts resorb bone. Osteoclasts are formed by the fusion of cells of the monocyte cell line. Osteoclasts are characterized by high expression of tartrate resistant acid phosphatase (TRAP) and cathepsin K.). "Five new oleanolic acid glycosides from Achyranthes

Bidentata with inhibitory activity on osteoclast formation." Planta Med 2005 July 71 (7): 673-9 "Analgestic effect of different processed products of Achyranthes bidentata in mice was observed in hot plate and acetic acid induced writhing test. The experiment results showed that water extract of Achyranthes bidentata and its processed products could inhibit the pain. The analgestic effect of its product processed with wine is the most powerful and lasting. The results showed that Achyranthes bidentata could obviously inhibit the ear inflammation of mice which was led by croton fruit oil. The anti-inflammatory effect of its product processed with wine is the most powerful." "The research on

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analgestic and anti-inflammatory action of different products of Achyranthes Bidentata" Zhong

Yao Cai. 1997 Oct;20(10):507-9 2. Semen Prunus Persica

Function of the herb: • For treatment of traumatic pain due to blood stasis

This herbal seed is rich in potassium that intervenes directly, together with the calcium content, in the process of bone formation. 3. Cortex Eucommia Ulmoides

Function of the herb: • Used to replenish liver and kidney • Strengthen bones and muscles • Treats aching back and knees • Tone up liver and kidneys • Strengthen tendon and bones

Historically, it has been commonly used for alleviating lower body pain and for weakness of the sinews and bones. This is a condition that arises with deficiency of the liver and kidney. "Aucubin and the other iridoids of eucommia are likely responsible for the anti-inflammatory effect, which is attained by inhibiting the arachidonic acid pathway" (Recio MC, Structural considerations on the

iridoids as anti-inflammatory agents, Planta Medica 1994; 60(3): 232-234 and Yue H, et al., Development of healthy beverage from Eucommia ulmoides foliage, Chinese Medicinal Herbs 1999; 22(8): 385-387).This may partly explain its use in treatment of arthritis. Eucommia was mentioned in the Shennong Bencao Jing "Eucommia is acrid and balanced. It mainly treats pain in the lower back and knees, supplements the center, boosts the essence qi, fortifies the sinews and bones, strengthens the will, and eliminates genital damp itch and dribbling urine after voiding. Protracted taking may make the body light and slow aging". 4. Fructus Psoralea Corylifolia

Function of the herb: • Used to warm up the kidney

The Chinese name for this herb is "bu gu zhi" meaning "tonify bone resin". Research has shown that it helps to stimulate bone formation. Because Psoralea promotes bone calcification it is useful in treating osteoporosis and osteoarthritis Research: A cell differentiation bioassay measuring alkaline phosphatase activity was developed using osteoblast-like UMR 106 cell line as a model. The effect of fractions and compounds of Psoralea corylifolia L. extract on osteoblastic differentiation was investigated. The fractions or compounds were co-cultured with cells for 48 h, the cellular ALP activity was then measured. The crude ethanol extract of Psoralea corylifolia L. increased ALP activity by 39.5% at a concentration of 0.1 mg/ml, and the activity was concentrated in the ethyl acetate fraction, which produced a maximum ALP activity increase of 38.2%. A significant activity was observed for a flavonoid, corylin, providing 30.0% increment in ALP. These effects suggest that Psoralea corylifolia L. extract, its ethyl acetate fraction and corylin would stimulate bone formation. "Osteoblastic differentiation bioassay

and its application to investigating the activity of fractions and compounds from Psoralea

corylifolia L." Pharmazie Vol 58 Issue 12 Dec 2003: 925-928.

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Research: Bakuchiol is a major component of Corylifoliae Fructus (Psoralea corylifolia L.) and has been clarified earlier to have antitumoral and cytotoxic activity. We have studied this molecule and it’s methoxy derivative to get the information that whether it can also be an antioxidant. It has been observed by biochemical assay that even at a very low concentration (IC50 = 8.0 m mol dm-3) it can inhibit lipid peroxidation.

Bakuchiol Methoxy bakuchiol

To gain more insight into the mechanism of antioxidant activity the reactions of .OH, N3., CCl3OO.,

GS. etc with bakuchiol has been studied and the bimolecular rate constant for reaction of these radicals are 3.6 x 109, 1.3 x 109, 2.8 x 108, and 9.7 x 108 dm3mol-1s-1, respectively. Methoxy-bakuchiol has been used to resolve the transients observed in the case of thiyl radical induced oxidation of bakuchiol. The bimolecular rate constant for the reaction of methoxy bakuchiol with thiyl radical is 2.8 x 108 dm3mol-1s-1. The phenolic hydrogen and the a -hydrogen with respect to the benzene ring are the two abstractable hydrogen atoms leading to two transients formed in the case of bakuchiol. Whereas in the case of methoxy bakuchiol it is the a -hydrogen with respect to the benzene ring abstracted to produce the transient. The mechanism has been supported by theoretical calculations. Both the semi-empirical as well as abinitio level calculations suggest that the radical formed due to hydrogen abstraction from a -position with respect to the benzene ring is the favoured one in the case of methoxy bakuchiol. It has also been observed that linoleate-peroxyl radical can efficiently cause oxidation of bakuchiol. From all these observations it can be concluded that at micro-molar concentration bakuchiol can be a potential antioxidant. "Pulse radiolysis study on the antioxidant

activity of bacuchiol: kinetic aspects." Radiation Chemistry & Chemical Dynamics Division,

Bio-Organic Division, Bhabha Atomic Research center, Mumbai 400 085, India 5. Herba Lycopus Lucidus

Function of the herb: • To invigorate blood circulation

Objective: To study the effect of active fractions from Lycopus lucidus (L. F04) on platelet aggregation and thrombus formation so as to investigate its mechanism of promoting blood

circulation and removing blood stasis. Method: The effects of Lycopus lucidus L. F04 on platelet aggregation induced by ADP in vivo, and thrombosis of artery-vein by-pass and thrombus formed in circuitous loop in vitro were examined using the blood stasis animal model of rats made by injecting high molecular weight dextran (HMWD). Result: The increase of maximum platelet aggregation rate induced by ADP in vivo in HMWD model was evidently inhibited with 0.408 g/kg or 0.204 g/kg or L. F04, and the effect showed a trend of concentration-dependence. As compared with the control, the thrombus weight in rat model of blood stasis increased clearly and its length showed only a trend of increase. Both 0.408 g/kg and 0.204 g/kg of L. F04 had the effect of resisting the thrombus formation, while 0.408 g/kg showed better effects of reducing the thrombus dry weight and wet weight. Both 0.408 g/kg and 0.204 g/kg of L. F04 could inhibit the thrombosis in artery-vein by-pass; the inhibition rates were 27.41% and 27.14% respectively. Conclusion: Lycopus lucidus L. F04 could significantly

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inhibit platelet aggregation and thrombus formation. "Effects of active fractions from Lycopus

lucidus L. F04 on platelet aggregation and thrombus formation." Space Med Med Eng (Beijing)

(2004) 17: 313-7 6. Radix Notopterygium Incisum

Function of the herb: • Used as anti-rheumatic and analgesic agent

Notopterol was identified as the analgesic component of Notopterygium incisum by using the acetic acid-induced writhing method. Notopterol also indicated an anti-inflammatory activity by its inhibitory effect in the vascular permeability test. The intensive prolongation of pentobarbital-induced hypnosis was possibly caused by its inhibitory effect on the drug metabolism in liver. Pharmacological differences between the analgesic components of N. incisum, Aralia cordata and Angelica pubescens were also discussed. "Analgesic component of Notopterygium incisum Ting"

Chem Pharm Bull (Tokyo). 1993 May;41(5):926-9


http://www.nutrihuang.com/Nerbonecare_Testimonials.htm

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10) Rener-Liv


1. Weak kidneys (lower back ache) 2. Osteoporosis (One of our herbs contains plant-like hormones and behaves like progesterone. It

helps to stimulate the absorption of calcium and our herbs have high colloidal calcium content. One of the herb’s calcium content can be as high as 40,000 ppm. Pure calcium compound like calcium carbonate are not well absorbed by the body)

4. Enhance women’s vitality (Not so tired and exhausted) 4. Increase body warmth (Ideal for those who constantly feel cold especially at night) 5. Excessive perspiration 6. Tonifies liver, kidney and spleen 7. Improves menstrual period 8. Purifies blood 9. Improves immunity

Herbs:

Poria Cocos Wolf Diospycos Kaki L Radix Polygonum Multiflorum Salvia Miltiorrhiza

1. Poria Cocos Wolf


• It has regulatory effect on human immune cells. "The structure of a new triterpene derivative isolated from Poria cocos was determined to be 3 beta-p-hydroxybenzoyldehydrotumulosic acid by spectral and chemical methods. 3 beta-p-hydroxybenzoyldehydrotumulosic acid showed marked inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)- and arachidonic acid (AA)-induced ear inflammation in mice. The 50% inhibitory doses of 3 beta-p-hydroxybenzoyldehydrotumulosic acid were 0.27 and 1.25 mg per ear on TPA- and AA-induced inflammation, respectively.".”3 beta-p-


Phytochemistry 1998 Aug;48(8):1357-60

"Pachymic acid, 3-O-acetyl-16 alpha-hydroxytrametenolic acid, and poricoic acid B had been isolated from the sclerotium of Poria cocos Wolf. These compounds showed a strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice. At 0.2 mumol/mouse, these compounds markedly inhibited the promoting effect of 12-O-tetradecanoylphorbol-13-acetate (1 microgram/mouse) on skin tumor formation following initiation with 7,12-dimethylbenz[a]anthracene (50 micrograms/mouse)." "Inhibitory effects of lanostane-type triterpene acids, the components


carcinogenesis in mouse skin." Oncology. 1996 Sep-Oct;53(5):382-5.

"Triterpene carboxylic acids were isolated from the methanol extract of Hoelen, Poria cocos, and found to inhibit 12-O-tetradecanoylphorbol 13-acetate (TAP)-induced mouse ear edema. Their

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chemical structures were identified as 3 beta,-16 alpha-dihydroxylanosta-7,9(11),24-trien-21-oic acid, 16 alpha-hydroxydehydropachymic acid, 16 alpha-hydroxytrametenolic acid and dehydrotumulosic acid." "Isolation of inhibitors of TPA-induced mouse ear edema from Hoelen, Poria cocos."

Chem Pharm Bull (Tokyo). 1996 Apr;44(4):847-9

"The antioxidant properties of twenty medical herbs used in the traditional Mediterranean and Chinese medicine were studied. Extracts from Forsythia suspensa, Helichrysum italicum, Scrophularia auriculata, Inula viscosa, Coptis chinensis, Poria cocos and Scutellaria baicalensis had previously shown anti-inflammatory activity in different experimental models. Using free radical-generating systems H. italicum. I. viscosa and F. suspensa protected against enzymatic and non-enzymatic lipid peroxidation in model membranes and also showed scavenging property on the superoxide radical. All extracts were assayed at a concentration of 100 microg/ml. Most of the extracts were weak scavengers of the hydroxyl radical and C. chinensis and P. cocos exhibited the highest scavenging activity. Although S. baicalensis inhibited the lipid peroxidation in rat liver microsomes and red blood cells, the extract showed inhibitory actions on aminopyrine N-demethylase and xanthine oxidase activities as well as an pro-oxidant effect observed in the Fe3+-EDTA-H2O2 system. The results of the present work suggest that the anti-inflammatory activities of the same extracts could be explained, at least in part, by their antioxidant properties." "Antioxidant activity of

anti-inflammatory plant extra." Life Sci. 2002 Jan 18;70(9):1023-33

"Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3 -hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells." "Inhibitory



Pharmaceutical University. 2. Diospycos Kaki L


• For treatment of constipation and haemorrhoids • For the treatment of bronchial complaints • For the treatment of hypertension • It is used to treat hiccups • It has anthelmintic, antihaemorrhagic, antivinous, expectorant, febrifuge and restorative

properties "The effect of dietary persimmon (Diospycos Kaki L)(Pers, 7.0%) on lipid metabolism and antioxidant activity was investigated in 40 male Wistar rats adapted to cholesterol-free or 1% cholesterol diets. The rats were divided in four groups of 10. The basal diet contained wheat starch, casein, soybean oil, and mineral and vitamin mixtures. The control group (C) consumed the basal diet. To the basal diet were added 7 g/100 g dry persimmon (Pers), 1 g/100 g cholesterol (Chol), or both (Chol/Pers). The experiment lasted 4 wk. Plasma total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), triglycerides (TG), total phospholipids (TPH), HDL phospholipids (HDL-PH), lipid peroxides (LP) and liver TC concentrations were measured. Groups did not differ before the experiment. In the Chol/Pers vs. Chol group, the persimmon-supplemented diet significantly

(P < 0.05) lessened the rise in plasma lipids due to dietary cholesterol: TC (3.88 vs. 4.88 mmol/L; 20%), LDL-C (2.24 vs. 3.27 mmol/L; 31%), TG (0.72 vs. 0.89 mmol/L; 19%), LP (2.20 vs. 3.25 mmol/L; 32%) and TC in liver (32.8 vs. 49.9 $mol/g; 34%), (P < 0.001). The Chol/Pers diet significantly reduced the decrease in HDL-PH due to dietary cholesterol (0.73 vs. 0.58 mmol/L; 25.8%, P < 0.001) and decreased the level of TPH (1.32 vs. 1.73 mmol/L; 23%, P < 0.001).

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Persimmon in rats fed the basal diet without cholesterol did not significantly affect the variables measured. These results demonstrate that persimmon possesses hypolipidemic and antioxidant

properties that are evident when persimmon is added to the diet of rats fed cholesterol. These properties are attributed to its water-soluble dietary fiber, carotenoids and polyphenols." "

Dietary

Persimmon Improves Lipid Metabolism in Rats Fed Diets Containing Cholesterol". Department

of Pharmaceutical Chemistry, School of Pharmacy, The Hebrew University of Jerusalem,

Jerusalem, Israel The Journal of Nutrition Vol. 128 No. 11 November 1998, pp. 2023-2027

3. Radix Polygonum Multiflorum


• To tone up liver and kidney and replenish their vital essence • Treat fatty liver, increase red blood cells and reduce blood fats • Exert same effect as adrenoticortical hormone • For aching back

• Treat tiredness and exhaustion • Anti aging

• Improve immune system through its antioxidant effect • Protect liver • Regulate and nourish blood

• Increase red blood cell production

"The extract of the root of Polygonum multiflorum exhibited a significant antioxidant activity assessed by the DPPH radical scavenging activity in vitro. The bioassay-guided fractionation of the extract yielded a stilbene glucoside, (E)-2,3,5,4'-tetrahydroxystilbene-2-O-beta-d-glucopyranoside (1) as an active constituent responsible for the antioxidant property. Compound 1 demonstrated a moderate DPPH radical scavenging activity (IC50, 40 microM), while the corresponding deglucosylated stilbene 2 exhibited a much higher activity (IC50, 0.38 microM)." "The radical

scavenging effects of stilbene glucosides from Polygonum multiflorum". Arch Pharm Res. 2002

Oct;25(5):636-9.



• Promote blood circulation • Treat coronary heart disease • Increase red blood cells • Protect liver • Improve function of liver and spleen

In one study Salvia Miltiorrhiza (Sm) administration has been shown to reduce hepatic fibrosis (improve liver function) in rats. We investigated the hemodynamic effects of Sm on bile duct ligated (BDL) rats. Hemodynamic, histological, and vascular contractile studies were conducted in rats 4 weeks after bile duct ligation. An aqueous extract of Sm (0.2 g twice per day) or vehicle was administered for 4 weeks to BDL rats. Sm treatment in BDL rats significantly reduced histological grades of fibrosis and ameliorated the portal hypertensive state (including portal venous pressure, superior mesenteric artery blood flow, cardiac index, and total peripheral resistance) as compared with vehicle treatment. Moreover, Sm treatment enhanced the vascular sensitivity of mesenteric arteries to phenylephrine in BDL rats. Sm treatment had no effect on plasma biochemical profiles of either BDL or normal rats. Our results suggest that 4-week Sm treatment ameliorates the portal hypertensive state in BDL rats. "Hemodynamic effects of Salvia miltiorrhiza on cirrhotic rats" Canadian Journal of

Physiology and Pharmacology, Volume 79, Number 7, July 2001, pp. 566-572(7) Salvia Miltiorrhiza has also been shown to be able to do the following:

Page 45

• Activates kallikrein-kinin system in the (rat) kidney to promote production and secretion of

prostaglandin E2, inducing dilation of renal vascular system, an increase in renal blood flow and glomerular filtration rate

• Increases coronary blood flow without affecting heart rate "Excessive oxidative stress is implicated in hepatic fibrogenesis. Extracts of Salvia miltiorrhiza (Sm) have been shown to protect cells against oxidative stress. In this study we investigated the in vitro and in vivo effects of Sm on hepatic fibrosis. A cell line of rat hepatic stellate cells (HSC-T6) was stimulated with transforming growth factor-beta1 (TGF-beta1). The inhibitory effects of Sm (50-400 microg/ml) on TGF-beta1-induced alpha-smooth muscle actin (alpha-SMA) secretion and the mRNA expressions of fibrosis-related genes, including alpha-SMA, connective tissue growth factor (CTGF), and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed. Fibrosis was induced by dimethylnitrosamine (DMN) administration in rats. DMN-treated rats were randomly assigned to 1 of 4 groups: saline, Sm (20 mg/kg), Sm (100 mg/kg), or silymarin (100 mg/kg), each given by gavage twice daily for 5 weeks starting from the onset of DMN administration. Sm (200 and 400 microg/ml) significantly inhibited TGF-beta1-stimulated alpha-SMA secretion and the mRNA expressions of alpha-SMA, CTGF, and TIMP-1 in HSC-T6 cells. Fibrosis scores of livers from DMN-treated rats with either a low (1.8 +/- 0.2) or high (1.8 +/- 0.1) dose of Sm, or silymarin (1.4 +/- 0.2) were significantly reduced in comparison with DMN-treated rats receiving saline (3.1 +/- 0.1). Hepatic collagen contents were also significantly reduced by either Sm or silymarin treatment. The mRNA expression levels of alpha-SMA, TGF-beta1, and procollagen I were all attenuated in Sm- and silymarin-treated rats. Moreover, levels of plasma aspartate transaminase activities were reduced by Sm and silymarin treatment. In conclusion, our results show that Sm exerted antifibrotic effects in both HSC-T6 cells and in rats with DMN-induced fibrosis." "Antifibrotic effects of Salvia

miltiorrhiza on dimethylnitrosamine-intoxicated rats". J Biomed Sci. 2005;12(1):185-95.



11(2), pp 102-4, 1991.




Overall Treatment Pathology

For kidney, liver and spleen function, lower backache, women feeling cold at night, pain of joints & bones due to osteoporosis, purify blood, children with retarded growth, enhance women's vitality and improves functions of the intestines. How does RENER-LIV treat osteoporosis? The herbs stimulate

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the liver and kidneys in the production of somatomedin. Somatomedin stimulates growth of bone and muscles and promotes cell growth and division and also influences calcium, phosphate, carbohydrate and lipid metabolism. That is why some experiences “puberty” type of sleep at night. It is like turning back the clock and experience a second slow rejuvenation. In TCM, kidneys govern the bones and marrow. Taken long-term it benefits the skin. For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/Rena-Liv_Testimonials.htm

11) Run On


1. Weak Legs

Herbs:

Drynariae Rhizoma Herba Cynomorium Songaricum Eucommia Ulmoides Gentiana Macrophylla 1. Drynariae Rhizoma


• Strengthen myoskeletal systems Scientific researches on Drynariae rhizome aka Rhizoma Gusuibu (the Chinese name translates to "Mender of Shattered Bones"), found that the herb assists and promotes the mending of sinews and bones. Drynariae Rhizoma (DR), a traditional Korea medicine, which is known for its effect to strengthen myoskeletal systems, frequently appears as the main ingredient in prescriptions for bone injuries. However, it is unclear how it pharmacologically contributes to the reformation of bone. In this study, the effect of DR on bone cells was investigated in vitro for the first time. The human osteoprecursor cells (OPC-1) were incubated in the medium with different concentrations of DR and the cell proliferation was studied. When the concentration of DR was < or = 120 microg ml(-1), the proliferation of OPC-1 was enhanced. However, the proliferation of OPC-1 was inhibited by DR with the concentrations of > 250 microg ml(-1). Under most treatments, the cells presented very pale expression for cyclooxygenase-2 protein; slightly intensified band showed at the highest DR concentration, 120 microg ml(-1) during the course of culture. On the other hand, we investigated the immunomodulatory activity of DR on cellular and humoral immunity. When different doses of ethanolic and water extracts of DR was administered to mice, it was dose-dependently potentiated the delayed-type hypersensitivity reaction induced by both sheep red blood cells (SRBC) and oxazolone. It significantly enhanced the production of circulating antibody titre in mice in response to SRBC. But, DR did not any effect on macrophage phagocytosis. Prolonged administration of DR significantly ameliorated the total white blood cell count and also restored the immunosuppressive effects induced by cyclophosphamide. The present investigation reveals that DR possesses immunomodulatory activity. From the results, it was concluded that DR directly stimulated the proliferation, alkaline phosphatase activity, protein secretion and particularly type I collagen synthesis of OPC-1 at dose-dependent manner, and stimulated both the cellular and the humoral immunity. “Effects of Drynariae rhizoma on the proliferation of human bone cells and the

immunomodulatory activity”. Department of Internal Medicine, Biochemistry and Molecular

Biology, College of Oriental Medicine, Dongguk University and National Research Laboratory

for Glycobiology, Sukjang-Dong 707, Kyungju, Kyungbuk 780-714, Republic of Korea.

Pharmacol. Res. (2005).

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Effects of traditional Korean medicine, Drynariae rhizoma (DR), on the protease activity of bone loss-initiation in rats and mice were investigated. Ethanol extracts-DR (EE-DR) and water extracts-DR (WE-DR) were identified as potent inhibitor of cathepsins K and L. The original WE-DR inhibits cathepsins K and L with IC50 values of 3.7 microg/ml and 4.5 microg/ml, respectively. EE-DR was more potent than that of WE-DR, because the inhibitions of cathepsin K and L increased to 0.5 microg/ml and 0.8 microg/ml, respectively. The EE-DR was proved to be the most potent. EE-DR was found to be a potent inhibitor of cathepsins K with a Kin value of 5.0 microg/ml for cathepsin K. The activity was increased by 10-fold when the assay is performed in the presence of glutathioneat pH 7.0, which favors the formation of a GSH thiolate anion. Thus, it is suggested that this increase in potency is probably due to an enhanced chemical reactivity of the extract mixtures toward the thiolate of the active site of the enzyme. WE-DR exhibited time-dependet inhibition which allowed us to determine the association and dissociation rate constants with cathepsin K. Finally, EE-DR inhibitsbone resorption in an in vitro assay involving mouse osteocleasts and bovine bone with an IC50 value of 70 microg/ml. WE-DR represents a new herbal formulation inhibiting cathepsin K and L activity and proteolysis of bone collagen. These results strongly suggest that DR is effective for preventing the development of bone loss induced by cathepsin K. This result also suggested that the DR is effective for bone resorptive action in bone cells. “Inhibitory activity of Drynariae rhizoma

extracts on cathespin having bone resorption activity”. Immunopharmacology and

immunotoxicology. (2004) In the traditional Korean medicine, Drynariae Rhizoma (DR) [Drynaria fortunei (kunze) J. Sm] has been reported as a good enhancer for bone healing. In this experiment, we investigate the effects of DR on bone resorption using the bone cells culture. Different concentrations of crude extract of DR were added to mouse bone cells culture. The mitochondria activity of the bone cells after exposure was determined by colorimetric MTT assay. It was demonstrated that DR has potential effects on the bone cells culture without any cytotoxicity. The most effective concentration of DR on bone cells was 100 micro g/ml. On the other hand, cathepsin K (Cat K) is the major cysteine protease expressed in osteoclasts and is thought to play a key role in matrix degradation during bone resorption. In this study, Mouse long bone cells including osteoclasts and osteoblast were treated with the PI3-kinase inhibitor, wortmannin (WT), and a specific inhibitor of protein kinase C (PKC), calphostin C. Although WT prevented the osteoclast-mediated intracellular processing of Cat K, calphostin C did not. Similarly, treatment of osteoclasts-containing long bone cells with Drynariae Rhizoma (DR) extracts prevented the intracellular maturation of Cat K, suggesting that DR may disrupt the intracellular trafficking of pro Cat K. This is similar to that of WT. Since secreted proenzymes have the potential to reenter the cell via mannose-6-phosphate (M6P) receptor, to prevent this possibility, we tested WT and DR in the absence or presence of M6P. Inhibition of Cat K processing by WT or DR was observed in a dose-dependent manner. Furthermore, the addition of M6P resulted in enhanced potency of WT and DR. DR dose-dependently inhibited in vitro bone resorption with a potency similar to that observed for inhibition of Cat K processing. “Inhibition of Drynariae

Rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse

osteoclasts”. Int Immunopharmacol 2003 Nov;3(12):1685-97 Pharmacological factors are needed to prevent bone loss that occurs with increasing age. The chemical compounds that act on bone metabolism in herbal medicines, however, are poorly understood. Effects of traditional Korean medicine, Drynariae Rhizoma [Drynaria fortunei (kunze) J. Sm] extract (DR), on the osteoblastic proliferation and differentiation were investigated. The effect of DR, a natural phyto herb, on the proliferation and osteoblastic differentiation in non-transformed osteoblastic cells (MC3T3-E1) was studied. DR dose-dependently increased DNA synthesis (significant at 50–150 µg/ml). DR increased alkaline phosphatase (ALP) activity and prolyl hydroxylase activity of MC3T3-E1 cells (50–150 µg/ml). Antiestrogen tamoxifen eleminated the stimulation of proliferation and ALP activity of MC3T3-E1, which were induced by DR. DR at concentrations ranged from 30–100 µg/ml inhibited prostaglandin E2 production in MC3T3-E1. These results indicate that DR directly stimulates cell proliferation and differentiation of osteoblasts. These results also suggest and DR is effective for bone anti-resorptive action in bone cells. “Stimulative effects of Drynariae Rhizoma extracts on the proliferation and differentiation of

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osteoblastic MC3T3-E1 Cells” Elsevier Science Ltd Journal of Enthnopharmacology Volume

96, Issue 3, 15 January 2005, Pages 489-495 In a previous study (Jeong et al., 2003, Inhibition of Drynariae Rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts. International Immunopharmacology 3, 1685-1697), treatment of osteoclasts-containing long bone cells with Drynariae Rhizoma (DR) extract prevented the intracellular maturation of cathepsin K and thus, it was considered that DR is a pro-drug of a potent bone resorption inhibitor. To further clarify the role of DR in ossification, we investigated the effects of DR on the proliferation and differentiation of osteoblastic cell lines in vitro. In this study, the bone effect of DR is studied. We assessed the effects of DR on osteoblastic differentiation in nontransformed osteoblastic cells (MC3T3-E1) and rat bone marrow cells. DR enhanced alkaline phosphatase (ALP) activity and mineralization in a dose- and time-dependent fashion. This stimulatory effect of the DR was observed at relatively low doses (significant at 50-150 $g/ml and maximal at 150 $g/ml). Northern blot analysis showed that the DR (100 $g/ml) increased in bone morphogenetic protein-2 as well as ALP mRNA concentrations in MC3T3-E1 cells. DR (60 $g/ml) slightly increased in type I collagen mRNA abundance throughout the culture period, whereas it markedly inhibited the gene expression of collagenase-1 between days 15 and 20 of culture. These results indicate that DR has anabolic effects on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases such as osteoporosis. “Drynariae Rhizoma promotes osteoblast

differentiation and mineralization in MC3T3-E1 cells through regulation of bone

morphogenetic protein-2, alkaline phosphatase, type I collagen and collagenase-1”. Elsevier

Science Ltd Toxicology in Vitro, 2004 (Vol. 18) (No. 6) 829-834 2. Herba Cynomorium Songaricum

Function of the herb: • To reinforce the vital function of kidney • For premature ejaculation

In order to exploit the medicinal resources and provide a scientific evidence for the clinical use and quality of Cynomorium songaricum control, the chemical constituents and pharmacological effects of the stem of the plant were studied preliminarily. METHOD: The chemical constituents were studied by spectral analysis and chemical method. The pharmacological effects were studied by an experiment on the defecation and deficiency of kidney function. RESULT AND CONCLUSION: Seven compounds were isolated from the stem of C. songaricum and identified as beta-sitosterol(I), palmitic acid (II), ursloic acid (III), daucosterol (IV), (-)-catechin (VI), naringenin-4'-O-pyranogluoside (VII) and succinicyacid (XII). Compounds VI and XII were isolated from this species for the first time. The pharmacological results show that the different extracts facilitate catharsis and replenish the deficiency of kidney yang. “Studies on chemical constituents and pharmacological

effects of the stem of Cynomorium songaricum Rupr”. Zhongguo Zhong Yao Za Zhi 1999

May;24(5):292-4, 318-9.

3. Cortex Eucommia Ulmoides

Function of the herb: • Used to replenish liver and kidney • Strengthen bones and muscles • Treats aching back and knees • Tone up liver and kidneys • Strengthen tendon and bones

The most interesting study was conducted on an herb called Eucommia. Eucommia contains chemicals that fit into the testosterone receptor and cause it to fire as if testosterone itself were present

Page 49

- causing androgenic, or male-creating changes. These phytoandrogens also increase the activity of real testosterone. The researchers describe a phenomenal synergy between chemicals in Eucommia, testosterone receptors, and testosterone. (see abstract of the study below). Eucommia's value for strengthening the male system is not news to Asian herbalists. The Chinese have been using it for millenia, and they call it Du Zhong. They use it for increasing libido and treating impotence, as well as for some kidney/adrenal complaints, high blood pressure, arthritis and connective tissue diseases. It is one of the 50 main herbs of Traditional Chinese Medicine (TCM), and it has a long history of safe use. The history of searching for plant testosterone is an interesting look into what scientists know, and what they think they know. The scientific literature of the 1950s is littered with failed attempts to find plant sources of testosterone. They all failed because they were looking for a molecule that was identical to human testosterone. No mamalian testosterone was found in plants, but plants do produce some other molecules that hit the testosterone receptors. It took a laboratory 'bioassay' to convince scientists of what the TCM practitioners had been saying all along. The study on Eucommia found that it contains two classes of compounds that pump up Testosterone activity - terpenoids and an unusual type of fatty acids. Therefore it is useful for strengthening weak limps. BACKGROUND: Plants containing compounds such as the isoflavonoids, with female hormone-like effects that bind to human estrogen receptors, are known. But none has been previously shown to have corresponding male hormone-like effects that interact with the human androgen receptor. Here, we report that the tree bark (cortex) of the Gutta-Percha tree Eucommia ulmoides possesses bimodal phytoandrogenic and hormone potentiating effects by lipidic components. METHODS: The extracts of E. ulmoides were tested using in-vitro reporter gene bioassays and in-vivo animal studies. Key compounds responsible for the steroidogenic effects were isolated and identified using solid phase extraction (SPE), high performance liquid chromatography (HPLC), thin layer chromatography (TLC), gas chromatography-mass spectroscopy (GC-MS), electron spray ionisation-mass spectroscopy (ESI-MS) and nuclear magnetic resonance (NMR). RESULTS: The following bioactivities of E. ulmoides were found: (1) a phenomenal tripartite synergism exists between the sex steroid receptors (androgen and estrogen receptors), their cognate steroidal ligands and lipidic augmenters isolated from E. ulmoides, (2) phytoandrogenic activity of E. ulmoides was mediated by plant triterpenoids binding cognately to the androgen receptor (AR) ligand binding domain. CONCLUSION: In addition to well-known phytoestrogens, the existence of phytoandrogens is reported in this study. Furthermore, a form of tripartite synergism between sex steroid receptors, sex hormones and plant-derived lipids is described for the first time. This could have contrasting clinical applications for hypogonadal- and hyperlipidaemic-related disorders. “Novel phytoandrogens and

lipidic augmenters from Eucommia ulmoides”. BMC Complimentary Altern Med 2007 Jan

29;7:3

The biologically active compounds and free radical-/ or reactive oxygen species (ROS)-/ scavenging effect of water extract from Du-zhong (WEDZ) were investigated. The WEDZ used included leaves, raw cortex, and roasted cortex. The hot water extract of Du-zhong leaves showed marked activity as a ROS scavenger, and the scavenging effect was concentration dependent. The extract of roasted cortex exhibited a modest scavenging effect on ROS, while the extract of raw cortex had the weakest scavenging effect. The scavenging activity of WEDZ on ROS was correlated to its protocatechuic acid (PCA) content. The content of PCA in Du-zhong determined by HPLC followed the order of leaves (17.17 mg/g) > roasted cortex (2.99 mg/g) > raw cortex (1.16 mg/g). The inhibitory activity of leaf extract of Du-zhong was stronger than that of PCA on the peroxidation of linoleic acid at the same concentration of 0.1 mg/mL. The results presented herein indicated that extract of Du-zhong could possibly. act as a prophylactic (remedy that prevents or slows the course of an illness or disease) agent to prevent free radical-related diseases. “Reactive oxygen species scavenging activity

of Du-zhong (Eucommia ulmoides oliv.) and its active compounds”. J Agric Food Chem. 2000

Aug;48(8):3431-6.

4. Radix Gentiana Macrophylla

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• Antirheumatic agent for treatment of rheumatic pain of the whole body • For allergic inflammation • Tones up the liver • Strengthen bones, tendons and ligaments

Gentiana macrophylla is categorized in the Materia Medica with herbs that expel wind-dampness. The liver is said to nourish the tendons and ligaments and Gentiana macrophylla is particularly used in cases where these connective tissues become hypercontracted due to insufficient nourishment (e.g., deficiency of liver yin), yielding spasms that run from the joints to the muscles. Vangermeersch L

and Sun Peilin, Bi-Syndromes, 1994 SATAS, Brussels, Belgium. Gentiana macrophylla is as often indicated for "bone fever" in TCM pharmacology. This strange term refers to syndromes with fever and sweating in which the person feels as if there is steam coming from the bones, heating up the skin. It is used by herbal scientists to nourish and strenghten bones, temdons and ligaments. State Administration of Traditional Chinese Medicine, Advanced

Textbook on Traditional Chinese Medicine and Pharmacology, (vol. 1) 1995-6 New World

Press, Beijing. For testimonies kindly go to this webpage for more information:

http://www.nutrihuang.com/Run%20On_Testimonials.htm

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12) Slym-Care


1. Weight Loss through Thermogenesis (fat burning)

Herbs:

Herba Schizonepetae Gardeniae Fructus Radix Glycyrrhiza Glabra Forsythiae Fructus

1. Herba Schizonepetae


• it acts on lungs and liver channel.

1) Scientific research showed that Chinese herbs are effective in promoting lipolysis (breakdown

of fat stored in fat cells). "Chinese herbal medicines, Inchinko-to, Bofu-tsusho-san (among

others contain the flowing herbs: Forsythiae Fructus, Glycyrrhizae Rheum Palmatum,

Gardeniae Fructus. and Schizonepetae Spica) and Dai-saiko-to, containing 3, 18 and 8

components, respectively, have since long been used as an anti-inflammatory, antipyretic,

choleretic and diuretic agent for liver disorders and jaundice, as an anti-obesity agent, a

hypocholesterolemic agent for liver disorders and a therapeutic and/or preventive agent for

cholesterol gallstone disease with hypertriglycerid-emia in China and Japan, respectively. In the

present study, we investigated the effects of these three herbal medicines in young male mice fed

a high-fat diet. Plasma levels of lipids and the numbers of the fatty droplets in the liver

cytoplasm were markedly lowered by the diets supplemented with three herbal medicines. The

liver weights and the body growth were reduced by the diet supplemented with Dai-saiko-to,

which slightly affected the concentrations of total protein, albumin, creatinine or calcium, and

the activity of lactate dehydrogenase. Thus, Dai-saiko-to, besides Bofu-tsusho-san, seems

effective in the activities of anti-obesity, anti-hyperlipidemia and anti-hyperlipids in liver

cytoplasm, when used carefully”. “Effects of three Chinese herbal medicines on plasma and

liver lipids in mice fed a high-fat diet.” Journal of Ethnopharmacology Volume 109, Issue 2, 19

January 2007, Pages 236-240 2. Gardeniae Fructus

1) Research by Korean scientists shows that Gardeniae Fructus can reduce blood fat. Research

shows that the hypolipidemic activity of GF and its component crocin may be due to the

inhibition of pancreatic lipase and crocin, and its metabolite, crocetin, can improve

hyperlipidemia. Source: “Antihyperlipidemic (reduce blood fat) effect of crocin isolated from

the fructus of Gardenia jasminoides and its metabolite Crocetin.” Biol Pharm Bull. 2005

Nov;28(11):2106-10

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2) Research by Japanese scientists shows that Gardeniae Frustus and Rheum Palmatum

promotes breaking down of lipids (e.g. fats and cholesterol). Pharmacological examination was

made on bile secretion in rats of "Inchinkoto", the most representative preparation for jaundice

in Chinese medicine, and on each of its three constituent crude drugs (Gardeniae Fructus,

Artemisiae Capillaris and Rheum Palmatum). The extract of Gardeniae Fructus hardly

increased bile secretion, while genipin (found in Gardeniae Fructus), an agiycone of geniposide

which is present in this crude drug, markedly increased it “Pharmacological Studies of

Gardeniae Fructus. II. Contribution of the Constituent Crude Drugs of Choleretic Activity of

"Inchinko-to" in Rats”. Journal of the Pharmaceutical Society of Japan Vol.96, No.2(19760225)

pp. 147-153

3) “This article discusses the effects of Fructus Gardeniae extract on hepatic function. Fructus

Gardeniae extract manifested no hepatotoxic effects on rats, as shown by alkaline phosphatase,

aspartate aminotransferase, and lactate dehydrogenase studies. Fructus Gardeniae extract

failed to activate the UDP-glucuronyltransferase system; whereas in hyperbilirubinemic state

the enzyme was activated, presumably by substrate induction. Fructus Gardeniae extract

increased the activity of UDP-glucose dehydrogenase, which would result in an increase in

availability of UDP-glucuronic acid intracellularly. BSP clearance study showed an unexpected

impairment of hepatic uptake of the dye after extract treatment. The action mechanism

involved in lowering of serum bilirubin level by Fructus Gardeniae extract may well be

complex; it is probably acting on a locus other than glucuronyl transferase.” Effects of Fructus

Gardeniae Extract on Hepatic Function The American Journal of Chinese Medicine (AJCM)

1977 Vol: 5 Issue: 3/4 (1977) Page: 241 - 255 3. Radix Glycyrrhiza Glabra

Function of the herb: • Used to invigorate functions of the heart and spleen for the treatment of symptoms due to

deficiency of vital energy of these viscera • Cleanse and protect the liver • Promotes production of red blood cells • Invigorates the function of the heart and the spleen • Improves the vital energy • Improves the immune system

1) Research by Japanese scientists showed that Glycyrrhizae Radix (GR) extract, Forsythiae

Fructus (FF) extract and Herbal Schizonepetae (HS) extract reduces the body weight in mice

after 8 weeks through lipolysis (breakdown of fat stored in fat cells). Source: “Thermogenic,

anti-obesity effects of bofu-tsusho-san in MSG-obese mice.” Int-J-Obes-Relat-Metab-Disord.

1995 Oct; 19(10): 717-22

2) Research showed that Chinese herbs are effective in the activities of anti-obesity, anti-

hyperlipidemia and anti-hyperlipids in liver cytoplasm. "Effects of Chinese herbal medicines on

plasma and liver lipids in mice fed a high-fat diet". Journal of Ethnopharmacology Volume 109,

Issue 2, 19 January 2007, Pages 236-240. 4. Forsythiae Fructus

Functions of the herb: • It has antipyretic and anti-inflammatory agent

Research by Japanese scientists has shown that this group of herbs Forsythiae Fructus,

Glycyrrhizae Rhei Rhizoma, Gardeniae Fructus and Schizonepetae Spica can be used to reduce

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weight in humans. Their blood triglycerides levels decreased significantly following

administration of these herbs. The decrease in appetite was considered to be due to activation of

the adrenaline. BETA.3 receptor as well as due to the psychotropic actions (affecting the mind

or mood or other mental processes) of Gardeniae Fructus. Source: “The Effect of

Bofutsushosan on Weight Reduction in Humans.” Japanese Journal of Oriental Medicine

VOL.56;NO.6;PAGE.933-939(2005)


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13) SpitalCare


1. Drooling 2. Sagging Bosom 3. White Sputum

Herbs:

Fructus Amomum Villosum Semen Dolichos Lablab Semen Coix Lachrimajobi Agastachis Herba Poria Cocos Wolf Semen Perilla Frutescens

1. Semen Amoni

Function of the herb: • Used to warm up the spleen

2. Semen Coix Lachrimajobi

Function of the herb: • Improve function of the spleen and dispel dampness • For treatment of abscess of the lung

3. Agastachis Herba Long ago in China, a lady by the name of Peilan lived with her sister-in-law in the same house. One day, Peilan suffered a sunstroke with headache, dizziness, palpitations and nausea. The sister-in-law offered to go to the mountain to pick some herbs for her. One the way back, a poisonous snake bit her. She died after reaching home, leaving the herbs for Peilan. On hearing the sad news, the village decided to name the plant after her Function of the herb:

• Used to transform dampness and stimulate the spleen

4. Poria Cocos Wolf


"The antioxidant properties of twenty medical herbs used in the traditional Mediterranean and Chinese medicine were studied. Extracts from Forsythia suspensa, Helichrysum italicum, Scrophularia auriculata, Inula viscosa, Coptis chinensis, Poria cocos and Scutellaria baicalensis had previously shown anti-inflammatory activity in different experimental models. Using free radical-

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generating systems H. italicum. I. viscosa and F. suspensa protected against enzymatic and non-enzymatic lipid peroxidation in model membranes and also showed scavenging property on the superoxide radical. All extracts were assayed at a concentration of 100 microg/ml. Most of the extracts were weak scavengers of the hydroxyl radical and C. chinensis and P. cocos exhibited the highest scavenging activity. Although S. baicalensis inhibited the lipid peroxidation in rat liver microsomes and red blood cells, the extract showed inhibitory actions on aminopyrine N-demethylase and xanthine oxidase activities as well as an pro-oxidant effect observed in the Fe3+-EDTA-H2O2 system. The results of the present work suggest that the anti-inflammatory activities of the same extracts could be explained, at least in part, by their antioxidant properties." “Antioxidant activity of

anti-inflammatory plant extra." Life Sci. 2002 Jan 18;70(9):1023-33 "Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3 -hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells." "Inhibitory



Pharmaceutical University. 5. Semen Perilla Frutescens

Function of the herb: • Used as diaphoretic to disperse the external wind and cold for the treatment of common cold

without sweat • Used as an antiasthamatic • Used as an expectorant • Dispels thin white phlegm • For stuffy feeling in the chest

The body needs fatty acids to survive and is able to make all but two of them: linoleic acid, in the omega-6 family and linolenic acid, in the omega-3 family. These two fatty acids must be supplied by the diet and are therefore considered essential fatty acids (EFAs). Perilla is rich in linolenic/alpha-linolenic acid (ALA). Alpha-linolenic acid is beneficial for strengthen the lungs. Since alpha-linolenic acid or ALA cannot be manufactured by the body it must be obtained from food. ALA, as well as the fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), belongs to a group of fatty acids called omega-3 fatty acids. EPA and DHA are found primarily in fish while ALA is highly concentrated in plants like perilla. Once ingested, the body converts ALA to EPA and DHA, the two types of omega-3 fatty acids more readily used by the body. If we can consume sufficient ALA then intake of EPA and DHA is optional. Omega-3 fatty acids have been shown to reduce inflammation and help prevent certain chronic diseases such as lung disease like asthma and arthritis. These essential fatty acids are highly concentrated in the brain and appear to be particularly important for cognitive "The leaves of Perilla frutescens Britton (Labiatae) are one of the most popular garnishes in Japan, used as an antidote for fish and crab meat allergy or as a food colorant. The present study was conducted to evaluate its anti-allergic effect and to identify its active constituents using mice ear-passive cutaneous anaphylaxis (PCA)-reaction. 48 hr after the cutaneous injection of anti-ovalbumin serum into the ears of mice, ovalbumin and evansblue dye were intravenously injected. Perilla was extracted with boiling water, and intraperitoneally injected 15 min before ovalbumin-treatment. Thirty min after ovalbumin-treatment, the ears were removed and the colorant in the ear was colorimetrically

Page 55

quantitated. Perilla extract significantly suppressed the PCA-reaction, which was brought about by rosmarinic acid with a partial contribution from some macromolecular compounds. The anti-allergic titer of rosmarinic acid was more effective than tranilast, which is a modern anti-allergic drug. Perilla and rosmarinic acid are potentially promising agents for the treatment of allergic diseases." "Anti-


Mar;17(3):240-3 Okamato M, et al.Dept of Medicine, Misasa Medical Branch, Tottori, Japan. Dietary sources of alpha-linolenic acid, such as perilla seed oil, may have the capacity to inhibit the generation of leukotrienes by leucocytes in patients with asthma, as has been reported with the consumption of other long-chain n-3 fatty acids. The factors affecting the suppression of leukotriene (LT) C4 generation by leucocytes were examined by comparing the clinical features of patients with asthma who had been given dietary perilla seed oil (n-3 fatty acids). Group A consisted of patients in whom the leucocytes generation of LTC4 was suppressed by dietary perilla seed oil. Group B consisted of those in whom LTC4 generation was not suppressed. Conclusions: Dietary supplementation with perilla seed oil in selected patients with asthma suppresses the generation of LTC4 and is associated with clinical features such as respiratory function and lipometabolism. “Effects of perilla seed oil supplementation on


Int Arch Allergy Immunol 2000 Jun; 122 (2) 137-42. Okamoto M. et al., Okayama University Medical School, Tohaku-gun, Tottori. The effects of perilla seed oil (n-3fatty acids) on bronchial asthma were compared with the effects of corn-oil (n-6 fatty acids) in relation to the pulmonary function and the generation of Leukotriene b4 (LTB4) and C4 (LTC4) by leucocytes. 14 asthmatics subjects were divided randomly into two groups: one group of 7 consumed perilla seed oil-rich supplementation and the other group of 7 consumed corn-oil rich supplementation for 4 weeks. Conclusion: The results suggest that perilla seed-oil-rich supplementation is useful for the treatment of asthma in terms of suppression of LTB4 and LTC4 generation by leucocytes, and improvement of pulmonary function. “Effects of dietary


Intern Med 2000 Feb; 39(2):107-11.


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14) Steve-Nek


1. Stiff Neck, 2. Muscle Stress and Ache Time to see results:

2 to 4 days

Herbs:

Caulis Uncaria Rhynchophylla Radix Paeonia Lactiflora Radix Ledebourillae Radix Bupleurum Scorzonerifolium

1. Uncaria Ramulus Cum Uncis

Function of the herb: • Antihypertensive and anticonvulsive agent for treatment of headaches, numbness of limbs

induced by hypertension • Reduced anxiety and blood pressure • Sedate and inhibit and counteract convulsion

"Uncaria rhynchophylla (Uncaria Ramulus Cum Uncis) is a medicinal herb which has sedative and anticonvulsive effects and has been applied in the treatment of epilepsy in Oriental medicine. In this study, the effect of alkaloid fraction of U. rhynchophylla against N-methyl-D-aspartate (NMDA)-induced neuronal cell death was investigated. Pretreatment with an alkaloid fraction of U. rhynchophylla for 1 h decreased the degree of neuronal damage induced by NMDA exposure in cultured hippocampal slices and also inhibited NMDA-induced enhanced expressions of apoptosis-related genes such as c-jun, p53, and bax. In the present study, the alkaloid fraction of U. rhynchophylla was shown to have a protective property against NMDA-induced cytotoxicity by suppressing the NMDA-induced apoptosis in rat hippocampal slices." "Alkaloid fraction of Uncaria

rhynchophylla protects against N-methyl-D-aspartate-induced apoptosis in rat hippocampal

slices." Neurosci Lett. 2003 Sep 4;348(1):51-5 "This study investigated the anticonvulsant effect of Uncaria rhynchophylla (UR) and the physiological mechanisms of its action in rats. A total of 70 male Sprague-Dawley (SD) rats were selected for study. Thirty four of these rats were divided into 5 groups as follows: 1) Control group (n = 6): received intraperitoneal injection (i.p.) of kainic acid (KA, 12 mg/kg); 2) URl000 group (n = 10), 3) UR500 group (n = 6) 4) UR250 group, received UR 1000, 500, 250 mg/kg i.p. 30 min prior to KA administration, respectively; 5) Contrast group: received carbamazepine 20 mg/kg i.p. 30 min prior to KA administration. Behavior and BEG were monitored from 15 min prior to drug administration to 3 hours after KA administration. The number of wet dog shakes were counted at 10 min intervals throughout the experimental course. The remaining 36 rats were used to measure the lipid peroxide level in the cerebral cortex one hour after KA administration. These rats were divided into 6 groups of 6 rats as follows: I) Normal group: no treatment was given; 2) Control group: received KA (12 mg/kg) i.p.; 3) UR l000 group, 4) UR500 group, 5) UR250 group, received UR

Page 57

1000, 500, 250 mg/kg i.p. 30 min prior to KA administration, respectively; 6) Contrast group: received carbamazepine 20 mg/kg i.p. 30 min prior to KA administration. Our results indicated that both UR 1000 and 500 mg/ kg decreased the incidence of KA-induced wet dog shakes, no similar effect was observed in the UR 250 mg/kg and carbamazepine 20 mg/kg group. Treatment with UR 1000 mg/kg, 500 mg/kg, or 250 mg/kg and carbamazepine 20 mg/kg decreased KA-induced lipid peroxide level in the cerebral cortex and was dose-dependent. These findings suggest that the anticonvulsant effect of UR possibly results from its suppressive effect on lipid peroxidation in the brain" "Anticonvulsant Effect of Uncaria rhynchophylla (Miq) Jack. in Rats with Kainic Acid-

induced Epileptic Seizure" The American Journal of Chinese Medicine, Vol. 27, No. 2 (1999)

257-26 "We have previously shown that an aqueous extract of the hooks and stems of Uncaria sinensis (Oliv.) Havil., Uncariae Uncus Cum Ramulusis, protects against glutamate-induced neuronal death in cultured cerebellar granule cells by inhibition of Ca2+ influx. Because it is not known which components of Uncaria sinensis are active, in this study we have evaluated, by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) staining, the neuroprotective effects of the oxyindole alkaloids corynoxeine, rhynchophylline, isorhynchophylline and isocorynoxeine, and the indole alkaloids geissoschizine methyl ether, hirsuteine and hirsutine, isolated from the hooks and stems of Uncaria sinensis, on glutamate-induced cell death. We also investigated the inhibitory effects of the compounds on 45Ca2+ influx in cultured rat cerebellar granule cells. Cell viability evaluated by the MTT assay was significantly increased by application of rhynchophylline (10(-3) M), isorhynchophylline (10(-4)-10(-3) M), isocorynoxeine (10(-4)-10(-3) M), hirsuteine (10(-4)-3 x 10(-4) M) or hirsutine (10(-4)-3 x 10(-4) M) compared with exposure to glutamate only, with the effect of isorhynchophylline being the strongest. The increased 45Ca2+ influx into cells induced by glutamate was significantly inhibited by administration of rhynchophylline (10(-3) M), isorhynchophylline (3 x 10(-4)-10(-3) M), isocorynoxeine (3 x 10(-4)-10(-3) M), geissoschizine methyl ether (10(-3) M), hirsuteine (3 x 10(-4)-10(-3) M) or hirsutine (3 x 10(-4)-10(-3) M). These results suggest that oxyindole alkaloids such as isorhynchophylline, isocorynoxeine and rhynchophylline and indole alkaloids such as hirsuteine and hirsutine are the active components of the hooks and stems of Uncaria sinensis which protect against glutamate-induced neuronal death in cultured cerebellar granule cells by inhibition of Ca2+ influx." "Evaluation of the protective effects of alkaloids isolated from the

hooks and stems of Uncaria sinensis on glutamate-induced neuronal death in cultured

cerebellar granule cells from rats" J Pharm Pharmacol. 1999 Jun;51(6):715-22. "In traditional Oriental medicine, Uncaria rhynchophylla has been used to lower blood pressure and to relieve various neurological symptoms. However, scientific evidence related to its effectiveness or precise modes of action has not been available. Thus, in the current study, we evaluated neuroprotective effects of U. rhynchophylla after transient global ischemia using 4-vessel occlusion model in rats. Methanol extract of U. rhynchophylla administered intraperitoneally (100-1000 mg/kg at 0 and 90 min after reperfusion) significantly protected hippocampal CA1 neurons against 10 min transient forebrain ischemia. Measurement of neuronal cell density in CA1 region at 7 days after ischemia by Nissl staining revealed more than 70% protection in U. rhynchophylla-treated rats compared to saline-treated animals. In U. rhynchophylla-treated animals, induction of cyclooxygenase-2 in hippocampus at 24 hr after ischemia was significantly inhibited at both mRNA and protein levels. Furthermore, U. rhynchophylla extract inhibited TNF-alpha and nitric oxide production in BV-2 mouse microglial cells in vitro. These anti-inflammatory actions of U. rhynchophylla extract may contribute to its neuroprotective effects." "Neuroprotection by methanol

extract of Uncaria rhynchophylla against global cerebral ischemia in rats" Life Sci. 2002 Apr

21;70(21):2467-80. "We isolated the phenolic compounds epicatechin, catechin, procyanidin B-1, procyanidin B-2, hyperin and caffeic acid from the hooks and stems of Uncaria sinensis (HSUS), and studied their protective effects against glutamate-induced neuronal death in cultured rat cerebellar granule cells. Cell viability evaluated by MTT assay was significantly increased by application of epicatechin (100-300 microM), catechin (300 microM), procyanidin B-1 (30-300 microM) and procyanidin B-2 (100-

Page 58

300 microM) compared with exposure to glutamate only. 45Ca2+ influx into cells induced by glutamate was also significantly inhibited by administration ofepicatechin (300 microM), catechin (300 microM), procyanidin B-1 (100-300 microM) and procyanidin B-2 (100-300 microM). These results suggest that epicatechin, catechin, procyanidin B-1 and procyanidin B-2 are the active components of HSUS that protect against glutamate-induced neuronal death in cultured cerebellar granule cells by inhibition of Ca2+ influx." "Protective effect of phenolic compounds isolated from

the hooks and stems of Uncaria sinensis on glutamate-induced neuronal death." Am J Chin

Med. 2001;29(1):173-80


Function of the herb: • Used to nourish the blood • Used as a spasmodic and pain relieving agent for headaches, abdominal pains, spasms of the

calf, muscles etc. The efficacy of white peony for relieving muscle cramps of various types, particularly combined with licorice in the formula shakuyaku-kanzo-to, is supported by several clinical trials. In a double-blind, placebo-controlled trial involving 101 patients with muscle cramps due to hepatic cirrhosis, dried extract of shakuyaku-kanzo-to was significantly superior to placebo in relieving symptoms over a two-week period. (21) The dose in this study -- 2.5 g three times daily before meals -- was sufficiently high to induce signs of pseudoaldosteronism (edema and weight gain) in five patients (9%) receiving shakuyaku-kanzo-to, due to the high intake of licorice. Other uncontrolled trials have shown that skakuyaku-kanzo-to can help relieve muscle cramps in people with diabetes mellitus, (22) those undergoing dialysis, (23) in alcoholics, (24) and in people with cerebrovascular disease. (25).

(21.) Kumada T, et al. Effect of shakuyaku-kanzo-to (Tsumura TJ-68) on muscle cramps

accompanying cirrhosis in a placebo-controlled double-blind parallel study. J Clin Ther Med

1999;15:499-523. (22.) Yosida M, et al. Effects of shakuyaku-kanzo-to on muscle cramp in diabetics. Neurol Ther

1995;12:529-534. (23.) Yamashita JI. Effect of Tsumura skakuyaku-kanzo-to on pain at muscle twitch during and

after dialysis in the patients undergoing dialysis. Pain & Kampo Medicine 1992;2:18-20. (24.) Maruyama K, et al. Effectiveness of shakuyaku-kanzo-to on convulsion and pain

associated with alcohol dependence. Kampo Igaku 1996;20:81-84. (25.) Sakamoto T, Hosino M. Effect of shakuyaku-kanzo-to extract granules on convulsion of

gastrocnemius muscle in patients with cerebrovascular disorder. Jpn J Oriental Med

1995;45:563-568.

3. Radix Ledebourillae

Function of the herb: • Used for stiff neck and back pain • Used as a spasmolytic • Used for relieving pain and anti rheumatic herb • It is used to treat peripheral neuroparalysis

4. Radix Bupleuri

Function of the herb: • Used to relieve stagnancy of vital energy to the liver • To elevate vital energy of the spleen • To reinforce the resistance of capillary vessels

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15) Stro-Care


1. Stroke Herbs:

Radix Polygonum Multiflorum Rhizoma Ligustic Chuan Xiong Rhizoma Atractylodis Macrocephalae Semen Ziziphi Spinosae Calculus Bovis Ramulus Cinnamoni Fructus Lycii Fructus Psoraleae

1) Radix Polygonum Multiflorum: Tonifies the liver and the kidneys and replenishes their vitality; treats fatty liver, increases red blood cells and reduces blood fats; exerts same effect as adrenocortical hormone; nourishes blood; for aching backs and knees; treats tiredness and exhaustion; protects heart from free radical damage. Polygonum Multiflorum: Research shows that "The extract of the root of Polygonum multiflorum exhibited a significant antioxidant activity assessed by the DPPH radical scavenging activity in vitro. The bioassay-guided fractionation of the extract yielded a stilbene glucoside, (E)-2,3,5,4'-tetrahydroxystilbene-2-O-beta-d-glucopyranoside (1) as an active constituent responsible for the antioxidant property. Compound 1 demonstrated a moderate DPPH radical scavenging activity (IC50, 40 microM), while the corresponding deglucosylated stilbene 2 exhibited a much higher activity" (IC50, 0.38 microM). “The radical scavenging effects of stilbene glucosides from Polygonum

multiflorum” Arch Pharm Res 2002 Oct;25(5):636-9.

2) Rhizorna Ligustic Chuanxiong: Invigorates blood circulation and promotes the flow of vital energy.

a) Ligustilide is the most abundant bioactive ingredient in Rhizoma Chuanxiong, a Chinese medicinal herb commonly used for the treatment of cardiovascular ailments. The present study reported, for the first time, the pharmacokinetics of ligustilide, administered in its pure form and in an herbal extract, in rats. After i.v. administration of pure ligustilide, it was distributed extensively (Vd, 3.76 ± 1.23 l/kg) and eliminated rapidly (t1/2,




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10.1124/dmd.107.017707


in blood vessels, a key factor for erection to take place: this is how Viagra works) via inhibiting

voltage dependent calcium channel and receptor-mediated Ca2+

influx and release.” Vascular

Pharmacology ISSN 1537-1891 2006, vol. 45, n 3, pp.171-176

c) Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5"105 µl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6±4.6% of the control to 12.5±2.1% in the presence of TMP (1.46 mM) and SAB (10 µM) (P<0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress. “Inhibition of shear-induced platelet aggregation in rat by tetramethylpyrazine and salvianolic

acid B, active component of Rhizoma Ligustici Chuanxiong”. Clinical Hemorheology and

Microcirculation Vol. 31, Number 2/2004 Pages 97-103

3) Rhizoma Atractylodis Macrocephalae: Tones up energy and strengthens the spleen. Protects the liver, promotes urination and reduces blood sugar.

4) Semen Ziziphi Spinosae: Nourishes blood and the liver. Traditional use to placate the heart and calm the spirit.

5) Ramulus Cinnamoni Cassia: Warms and clears up blood channels and promotes blood circulation for amenorrhoea (cessation of normal menstruation) and angina pectoris.

6) Fructus Lycii Barbarum: Replenishes the vital essences of the liver and the kidneys

7) Fructus Psoraleae Corylifolia: Warms up the kidneys to reinforce the vital functions of the sexual organs.

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a) Clinical studies by western scientists have shown that Radix Angelicae Sinensis, Radix

Rubiae and Fructus Psoraleae can improve kidney functions. The results before and after

treatment showed an evident difference, with low function before treatment and normal after

treatment. Forty-one children with idiopathic thrombocytopenic purpura (ITP) were reported in this paper. Of all, 36 with petechia and ecchymosis, 28 with nasal hemorrhage, 5 with hematochezia and 1 with subcutaneous hematoma. According to the TCM differentiation, 24 and 17 of all belonged to the type of impairment of Yin due to blood-heat and both deficiencies of Qi and tonifying Kidney and its basic prescriptions were: dried Radix Rehmanniae, Radix Angelicae Sinensis, Radix Paeoniae Rubra, Radix Rubiae, Fructus Psoraleae, stir-frying Radix Astragali, Semen Cuscutae, Caulis Spatholob; Chinese Jujubae, Radix et Rhizoma Rhei. The average treating duration was 5.02 months. Results: 24 cases cured, 6 recovered, 10 improved, 1 failed, with a total effective rate of 97.6%. 22 of the cured were followed-up without recurrence in average 10.4 months after withdrawal. In 32 cases, the thrombocyte aggregative function was tested with ADP induction, meanwhile in 20 of 32 cases aggregation with adrenaline induction was tested. The results before and after treatment showed an evident difference, with low function before treatment and normal after treatment. It is concluded that the replenishing Qi and tonifying Kidney may have a better effect on both acute and chronic children ITP without recurrence after withdrawal. “Idiopathic thrombocytopenic purpura in children

treated with replenishing qi and tonifying kidney and the changes in thrombocyte aggregative

function” Zhong Xi Yi Jie He Za Zhi. 1991 Jan;11(1):14-6, 3.

b) Clinical studies by western scientists have shown that Psoralea corylifolia stimulates bone

formation. Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L” A cell differentiation bioassay measuring alkaline phosphatase activity was developed using osteoblast-like UMR 106 cell line as a model. The effect of fractions and compounds of Psoralea corylifolia L. extract on osteoblastic differentiation was investigated. The fractions or compounds were co-cultured with cells for 48 h, the cellular ALP activity was then measured. The crude ethanol extract of Psoralea corylifolia L. increased ALP activity by 39.5% at a concentration of 0.1 mg/ml, and the activity was concentrated in the ethyl acetate fraction, which produced a maximum ALP activity increase of 38.2%. A significant activity was observed for a flavonoid, corylin, providing 30.0% increment in ALP. These effects suggest that Psoralea corylifolia L. extract, its ethyl acetate fraction and corylin would stimulate bone formation. “Osteoblastic differentiation bioassay and its application to investigating the activity of fractions

and compounds from Psoralea corylifolia L.” School of Pharmacy, Shenyang Pharmaceutical

University 103 Wenhua Road, Shenyang, 110016, China ISSN:0031-7144 Vol. 58 Issue 12


http://www.nutrihuang.com/Stro-Care_Testimonials.htm

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16) Warm-Rener


1. Frequent Urination (weak renal pelvis) 2. Incontinence 3. Lower backache 4. Degenerative Diseases

Herbs:

Radix Lindera Fructus Psoraleae Fructus Corni Fructus Rubi Radix Angelica Sinensis

1. Radix Linderae

Function of the herb: • For treatment of distension with pain in the lower abdomen due to cold. • To warm up the kidney and disperse cold • Treats frequent urination and urinary incontinence along with possible weak low back with

cold and soreness • Strengthen the will

1) Clinical studies by western scientists have shown that Radix Linderae contains PEP (Prolyl

endopeptidase) inhibitors that can improve the functions of proline-containing neuropeptides,

e.g., vasopressin "kidney hormone", substance P, and thyrotropin-releasing hormone "brain

hormone" (TRH). These neuropeptides are involved in the processes of learning and memory,

renal and other physiological activities. (Neuropeptides are neuroactive molecules which helps to coordinate, integrate, and regulate physiological processes in all organisms, throughout all phases of development. Acting as neurohormones, neurotransmitters, and/or neuromodulators, they maintain physiological homeostasis and influence important behavioral patterns.) "In Japan and China, Radix Linderae are used as a traditional medicine as an analeptic (stimulates the central nervous system) and analgesic (pain killer)....Prolyl endopeptidase (PEP, EC 3.4.21.26) is a serine protease cleaving peptide bond at the carboxyl side of proline residues and was first isolated as an oxytocin-inactivating enzyme. This enzyme is distributed in a wide range of species, especially in the human brain. Prolyl endopeptidase (PEP, EC 3.4.21.26) has been proposed to play a role in degradation (digestion) of proline-containing neuropeptides. In the central nervous system, PEP degrades (digests) proline-containing neuropeptides involved in the processes of learning and memory, e.g., vasopressin, substance P, and thyrotropin-releasing hormone (TRH). In the course of our search for bioactive constituents in medicinal plants, we studied the PEP inhibitory constituents of the roots of Lindera strychnifolia F. VILL and isolated two known tannins, epicatechin (1) and aesculitannin B (2), and four known sesquiterpenes, linderene (3), linderene acetate (4), linderalactone (5) and isolinderalactone (6) as inhibitors. On the inhibitory activities of six compounds against PEP from Flavobacterium meningosepticum and that from rat brain supernatant, compounds 1, 2 and 4 inhibited the enzyme from Flavobacterium more strongly than that from rat brain supernatant. However, compounds 3, 5 and 6 inhibited the enzymes from both origins to the same extent and furthermore,

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compound 6 was the strongest natural inhibitor against PEP from rat brain supernatant. The kinetic study of these inhibitors indicated that compounds 1, 2 are noncompetitive inhibitors and compounds 3-6 are competitive inhibitors. This is the first example of non-phenolic constituents showing significant competitive inhibitory activity being isolated from natural medicines." "Prolyl

endopeptidase inhibitors from the roots of Lindera strychnifolia F. Vill." Biol Pharm Bull. 2002

Aug;25(8):1049-52

2) Clinical studies by European scientists have shown that the destructions of neuropeptides,

thyrotrophin-releasing hormone (TRH) and arginine-vasopressin (AVP), by prolyl

endopeptidase can be inhibited by PEP (Prolyl endopeptidase) inhibitors. PEP (Prolyl

endopeptidase) inhibitors can be found in plants like Radix Linderae “Compound S 17092 is a potent and selective inhibitor of prolyl endopeptidase (EC 3.4.21.26, PEP) that may be of therapeutic value for the treatment of memory impairment associated with neurodegenerative diseases. In the present study, we investigated the effects of S 17092 on the catabolism of the promnesic neuropeptides thyrotrophin-releasing hormone (TRH) and arginine-vasopressin (AVP) in the rat brain. In vitro, bacterial PEP hydrolysed both TRH and AVP, and the breakdown of the two peptides was almost completely prevented by 10(-5) M S 17092. In vivo, a single oral administration of S 17092 provoked a significant increase in TRH-like immunoreactivity (TRH-LI) in the cerebral cortex (+63% for a 10 mg/kg dose and +72% for a 30 mg/kg dose), as well as AVP-LI in the hippocampus (+54% for a 30 mg/kg dose), but did not affect TRH-LI in the amygdala nor AVP-LI in the cerebral cortex. Chronic administration of S 17092 (10 or 30 mg/kg daily) lead to a significant increase in THR-LI in the cerebral cortex (+55% and +56%, respectively), but did not modify AVP-LI in the hippocampus, nor in the cerebral cortex. These results show that the selective PEP inhibitor S 17092 increases TRH and AVP content in discrete regions of the rat brain. The present data suggest that the promnesic and antiamnesic effects of S 17092 can be accounted for, at least in part, by blockage of AVP and TRH degradation by PEP”. “Effect of prolyl endopeptidase inhibition on arginine-vasopressin and

thyrotrophin-releasing hormone catabolism in the rat brain.” J Neuroendocrinol. 2005

May;17(5):306-13.

3) Research scientists from Japan said Lindera strychnifolia, a medicinal plant, has long been

used for the treatment of cardiac, renal and rheumatic diseases. “Lindera strychnifolia (tendai-uyaku), a medicinal plant, has long been used for the treatment of cardiac, renal and rheumatic diseases in Japan. We aim to clarify (1) whether L. strychnifolia is protective against post-ischemic myocardial dysfunction, and (2) whether its effect is related to scavenging hydroxyl radicals and opening the mitochondrial KATP channels in isolated rat hearts. Male Sprague-Dawley rats were orally given 1 ml/day of L. strychnifolia, which was extracted from 0.75 and 1.5 g/kg of roots of L. strychnifolia for 4 days. The rat hearts were excised and perfused on a Langendorff apparatus with Krebs-Henseleit solution with a gas mixture of 95% O2 and 5% CO2. The hearts were paced at 320 beats/min except during ischemia. Left ventricular developed pressure (LVDP, mmHg), +/- dP/dt (mmHg/sec) and coronary flow (ml/min) were continuously monitored. All hearts were perfused for a total of 120 minutes consisting of a 30-minute pre-ischemic period followed by 30 minutes of global ischemia and 60 minutes of reperfusion with or without 5-HD, a mitochondrial KATP channel blocker. The levels of lactate, LDH and 2,5-DHBA, an indicator of hydroxyl radicals, in the perfusate during reperfusion period were also measured. Treatment with L. strychnifolia significantly improved LVDP and +/- dP/dt without altering coronary flow during reperfusion. The 100 microM of 5-HD in Krebs-Henseleit solution was perfused during the 10 minutes of pre-ischemic periods. Pretreatment with 5-HD abolished the improvement of LVDP and +/- dP/dt by L. strychnifolia. L. strychnifolia significantly attenuated the levels of lactate, LDH and 2,5-DHBA during reperfusion, and which were restored by pretreatment with 5-HD. In conclusion, L. strychnifolia is protective against post-ischemic left ventricular dysfunction through scavenging hydroxyl radicals and opening the KATP channels in the isolated rat heart.” “Lindera strychnifolia is protective against post-ischemic myocardial

dysfunction through scavenging hydroxyl radicals and opening the mitochondrial KATP

channels in isolated rat hearts.” Am J Chin Med 2004; 32(4):587-98

4) Clinical studies by western scientists have shown that the extract of Radix Linderae (RL)

slowed down the progression of diabetic nephropathy (disease of the kidney). It resulted in a

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better renal function as evaluated by creatinine clearance (Ccr) and serum creatinine. Kidneys

of the RL extract-treated group showed glomeruli with greater area and cell population etc. “We examined a possible preventive effect of Linderae radix (LR), the root of Lindera strychnifolia, on the progression of diabetic nephropathy. Water extract of Linderae radix (LR extract) was orally administered to the C57BL/KsJ-db/db (db/db) mice, a model of genetic diabetes, at a dose of 730 mg/kg/day for 12 week. The LR extract treatment did not affect glucose metabolism and systolic pressure. However, it resulted in a better renal function as evaluated by creatinine clearance (Ccr) and serum creatinine than the control; Ccr and serum creatinine were progressively worsened in controls (0.13+/-0.01 (l/day) and 0.69+/-0.04 (mg/dl), respectively) whereas unchanged in the treated group (0.24+/-0.03 (l/day), p<0.05 and 0.53+/-0.04 (mg/dl), p<0.05, respectively). Kidneys of the LR extract-treated group showed glomeruli with greater area and cell population, smaller glomerular sclerotic index, and less fibrosis in glomeruli, where apoptotic rate of glomerular cells were decreased compared with the control kidneys. Furthermore, renal TGF-beta(1) expression was decreased in the LR extract-treated group. Kidneys of the LR extract-treated group showed glomeruli with greater area and cell population.” “Water extract of the root of Lindera strychnifolia slows down the

progression of diabetic nephropathy in db/db mice.” Life Sci. 2005; 77(12) 1391-403

5) Research scientists from Japan confirmed Lindera strychnifolia has analgesic and anti-

inflammatory action. “Both of aqueous extract and alconol extract of Radix Linderae at a dose 5 g/kg and 10 g/kg could length obviously pain threshold in mice in hot plate test. The samples at a dose 20 g/kg could inhibit significantly the writhing frequency induced by potassium antimony tartate in mice, also antagonize the swelling of ear induced by inflammatory agent and decrease the swelling rate. The component further isolated from the plant could antagonize the swelling of rat toes induced by carrageenin.” “Studies on the analgesic and anti-inflammatory action of radix Linderae

extract” Zhong Yao Cai 1997; 20 (12): 629-31 6) Clinical studies by western scientists have shown the levels of tissue

prolyl endopeptidase

(PEP) activity were highest in kidney of adult rats. “The distribution and ontogeny of tissue prolyl endopeptidase and pyroglutamyl peptidase I activities were studied in the rat from the 7th day before birth to adulthood. While low levels of prolyl endopeptidase activity were demonstrable in many fetal tissues, activity in brain cortex, hypothalamus, lung, and kidney increased dramatically during the 2 wk after birth, gradually returning to adult levels. In adult rats, levels of tissue

prolyl endopeptidase

activity were highest in kidney, when compared with the intermediate levels in brain cortex, hypothalamus, and liver. Pyroglutamyl peptidase activity was widely distributed in adult rat tissues

with high levels in kidney and liver that exceeded intermediate levels in brain cortex and hypothalamus. Pyroglutamyl peptidase activities in fetal gut, brain, and lung tissue were elevated above adult values. In contrast to the development changes in prolyl endopeptidase activities, pyroglutamyl peptidase activity remained elevated above adult levels only during the first week of life. These results indicate that both prolyl endopeptidase and pyroglutamyl peptidase activities in the rat are developmentally regulated” “Distribution and ontogeny of thyrotropin-releasing hormone

degrading enzymes in rats.” Am J Physiol Endocrinol Metab 259: E787-E791, 1990; 0193-

1849/90

7) Clinical studies by western scientists have shown that Prolyl endopeptidase is a proteolytic enzyme (enzyme that digests protein) which could have a neuropeptide catabolising (destructive) role in the central nervous system. The activity of the enzyme was higher in the human than in the rat brain. Prolyl endopeptidase enzyme are also found in the kidneys. “Prolyl endopeptidase is a proteolytic enzyme (enzyme that digest protein) which could have a neuropeptide catabolising role in the central nervous system. Although prolyl endopeptidase has been described as a cytosolic enzyme, it has become clear that it can also be found in particulate form. The regional and subcellular distribution of this enzyme was evaluated in rat and human brain. The activity of the enzyme was higher in the human than in the rat brain. In the human brain, the activity levels of both soluble and particulate prolyl endopeptidase were the highest in frontal, parietal and occipital cortices and the lowest in the cerebellum. In the rat brain, the regional distribution of the enzyme was more homogeneous. The activity in all the areas of the central nervous system is higher than in peripheral tissues. Subcellular

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distribution of the enzyme in the brain indicates that prolyl endopeptidase was higher in the cytosolic fraction than in the particulate fractions. The particulate form was enriched in the synaptosomal and the myelinic membranes. The high activity of prolyl endopeptidase in the human cortex suggests that prolyl endopeptidase could play a role in the functions of this brain area.” “Distribution of prolyl

endopeptidase activities in rat and human brain.” Neurochem Int. 2002; 40(4):337-45

2. Fructus Psoraleae

Function of the herb: • It is used to warm up the kidneys for the treatment of frequent urination and uncontrollable

urination. • Strengthen the bones

Clinical studies by western scientists have shown that Radix Angelicae Sinensis, Radix Rubiae

and Fructus Psoraleae can improve kidney functions. The results before and after treatment

showed an evident difference, with low function before treatment and normal after treatment. Forty-one children with idiopathic thrombocytopenic purpura (ITP) were reported in this paper. Of all, 36 with petechia and ecchymosis, 28 with nasal hemorrhage, 5 with hematochezia and 1 with subcutaneous hematoma. According to the TCM differentiation, 24 and 17 of all belonged to the type of impairment of Yin due to blood-heat and both deficiencies of Qi and tonifying Kidney and its basic prescriptions were: dried Radix Rehmanniae, Radix Angelicae Sinensis, Radix Paeoniae Rubra, Radix Rubiae, Fructus Psoraleae, stir-frying Radix Astragali, Semen Cuscutae, Caulis Spatholob; Chinese Jujubae, Radix et Rhizoma Rhei. The average treating duration was 5.02 months. Results: 24 cases cured, 6 recovered, 10 improved, 1 failed, with a total effective rate of 97.6%. 22 of the cured were followed-up without recurrence in average 10.4 months after withdrawal. In 32 cases, the thrombocyte aggregative function was tested with ADP induction, meanwhile in 20 of 32 cases aggregation with adrenaline induction was tested. The results before and after treatment showed an evident difference, with low function before treatment and normal after treatment. It is concluded that the replenishing Qi and tonifying Kidney may have a better effect on both acute and chronic children ITP without recurrence after withdrawal. “Idiopathic thrombocytopenic purpura in children

treated with replenishing qi and tonifying kidney and the changes in thrombocyte aggregative

function” Zhong Xi Yi Jie He Za Zhi. 1991 Jan;11(1):14-6, 3.

Clinical studies by western scientists have shown that Psoralea corylifolia stimulates bone

formation. Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L” A cell differentiation bioassay measuring alkaline phosphatase activity was developed using osteoblast-like UMR 106 cell line as a model. The effect of fractions and compounds of Psoralea corylifolia L. extract on osteoblastic differentiation was investigated. The fractions or compounds were co-cultured with cells for 48 h, the cellular ALP activity was then measured. The crude ethanol extract of Psoralea corylifolia L. increased ALP activity by 39.5% at a concentration of 0.1 mg/ml, and the activity was concentrated in the ethyl acetate fraction, which produced a maximum ALP activity increase of 38.2%. A significant activity was observed for a flavonoid, corylin, providing 30.0% increment in ALP. These effects suggest that Psoralea corylifolia L. extract, its ethyl acetate fraction and corylin would stimulate bone formation. “Osteoblastic differentiation bioassay and its application to investigating the activity of fractions

and compounds from Psoralea corylifolia L.” School of Pharmacy, Shenyang Pharmaceutical

University 103 Wenhua Road, Shenyang, 110016, China ISSN:0031-7144 Vol. 58 Issue 12

3. Fructus Corni

Function of the herb: • Used to replenish the vital essence of the liver and kidney. • Used to treat frequent urination.

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Clinical results demonstrate that Fructus corni can promote a protective antioxidant defense



damage in the kidneys. The present study determined the effects of Fructus corni extract (FCE) on the levels of hydrogen peroxide (H2O2) and superoxide anion (O2-), on the glutathione (GSH) redox cycle and on the activities of antioxidant enzymes in bovine pulmonary artery endothelial cells (PAECs). 2. Confluent monolayers of PAECs were incubated with FCE, and oxidative stress was triggered by hypoxanthine and xanthine oxidase (to induce H2O2) or H2O2 (to induce O2-). 3. FCE exhibited a concentration-dependent suppression of H2O2 and O2-. 4. It modulated the GSH redox cycle by increasing the intracellular GSH content, the activities of GSH peroxidase and GSH disulfide reductase, and by decreasing the intracellular level of GSH disulfide. 5. It also increased the activities of superoxide dismutase and catalase. 6. These results demonstrate that FCE can promote a protective antioxidant defense state by affecting some important enzymatic and nonenzymatic oxidant-scavenging systems and may thus be useful for the prevention or treatment of disorders associated with oxidative damage. “Fructus corni enhances endothelial cell antioxidant defenses”. Gen

Pharmacol. 1998; 31(2):221-5

4. Fructus Rubi

Function of the herb: • Used for treatment of frequent urination 5. Radix Angelica Sinensis

Function of the herb: • Nourish the blood • Invigorate the blood circulation

Clinical studies by western scientists have shown that Radix Angelicae Sinensis has

hematopoietic effect (formation of blood cellular components) on person suffering from anemia

cause by chronic renal failure. Therefore result showed that Radix Angelicae Sinensis improves

kidney functions. The primary cause of anemia of chronic renal failure (CRF) is insufficient production of erythropoietin by the diseased kidneys. Although most patients receiving hemodialysis (HD) respond to recombinant human erythropoietin (rHuEPO) treatment, 4% prove to be rHuEPO resistant. The patient presented in this case study suffered from anemia of CRF that was resistant to rHuEPO therapy. He experienced marked improvement in the anemia after self-initiating regular consumption of the Chinese herb Dang qui (botanical name: Radix angelicae sinensis). The significant hematologic amelioration occurred in the face of a major decrease in the amount of rHuEPO administered. The causes and mechanisms of inadequate response to rHuEPO, as well as the possible beneficial hematopoietic effects of this herbal preparation, are discussed. “Hematopoietic

effect of Radix angelicae sinensis in a hemodialysis patient “Am J Kidney Dis. 1999 Aug;

34(2):349-54


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17) Uri-X


1. Uric Acid 2. Gout

Herbs:

Poria Cocos Wolf Radix Gentiana Macrophyllae Radix Ledebouriella Divaricata Herba Lysimachia Christinae Herba Artemesia Scoparia Caulis Uncaria Rhynchophylla Herba Taraxacum Mongolicum Radix Salvia Miltiorrhiza

1. Poria Cocos Wolf


"The structure of a new triterpene derivative isolated from Poria cocos was determined to be 3 beta-p-hydroxybenzoyldehydrotumulosic acid by spectral and chemical methods. 3 beta-p-hydroxybenzoyldehydrotumulosic acid showed marked inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)- and arachidonic acid (AA)-induced ear inflammation in mice. The 50% inhibitory doses of 3 beta-p-hydroxybenzoyldehydrotumulosic acid were 0.27 and 1.25 mg per ear on TPA- and AA-induced inflammation, respectively." "3 beta-p-


Phytochemistry 1998 Aug;48(8):1357-60 "Pachymic acid, 3-O-acetyl-16 alpha-hydroxytrametenolic acid, and poricoic acid B had been isolated from the sclerotium of Poria cocos Wolf. These compounds showed a strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice. At 0.2 mumol/mouse, these compounds markedly inhibited the promoting effect of 12-O-tetradecanoylphorbol-13-acetate (1 microgram/mouse) on skin tumor formation following initiation with 7,12-dimethylbenz[a]anthracene (50 micrograms/mouse)." "Inhibitory effects of lanostane-type triterpene acids, the components


carcinogenesis in mouse skin." Oncology. 1996 Sep-Oct;53(5):382-5. "Triterpene carboxylic acids were isolated from the methanol extract of Hoelen, Poria cocos, and found to inhibit 12-O-tetradecanoylphorbol 13-acetate (TAP)-induced mouse ear edema. Their chemical structures were identified as 3 beta,-16 alpha-dihydroxylanosta-7,9(11),24-trien-21-oic acid, 16 alpha-hydroxydehydropachymic acid, 16 alpha-hydroxytrametenolic acid and dehydrotumulosic acid." "Isolation of inhibitors of TPA-induced mouse ear edema from Hoelen, Poria cocos."

Chem Pharm Bull (Tokyo). 1996 Apr;44(4):847-9

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"The antioxidant properties of twenty medical herbs used in the traditional Mediterranean and Chinese medicine were studied. Extracts from Forsythia suspensa, Helichrysum italicum, Scrophularia auriculata, Inula viscosa, Coptis chinensis, Poria cocos and Scutellaria baicalensis had previously shown anti-inflammatory activity in different experimental models. Using free radical-generating systems H. italicum. I. viscosa and F. suspensa protected against enzymatic and non-enzymatic lipid peroxidation in model membranes and also showed scavenging property on the superoxide radical. All extracts were assayed at a concentration of 100 microg/ml. Most of the extracts were weak scavengers of the hydroxyl radical and C. chinensis and P. cocos exhibited the highest scavenging activity. Although S. baicalensis inhibited the lipid peroxidation in rat liver microsomes and red blood cells, the extract showed inhibitory actions on aminopyrine N-demethylase and xanthine oxidase activities as well as an pro-oxidant effect observed in the Fe3+-EDTA-H2O2 system. The results of the present work suggest that the anti-inflammatory activities of the same extracts could be explained, at least in part, by their antioxidant properties." “Antioxidant activity of

anti-inflammatory plant extra." Life Sci. 2002 Jan 18;70(9):1023-33 "Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3 -hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells." "Inhibitory



Pharmaceutical University. 2. Radix Gentiana Macrophylla

Function of the herb: • Antirheumatic agent for treatment of rheumatic pain of the whole body • For allergic inflammation • Tones up the liver

3. Radix Ledebouriella Divaricata

Function of the herb: • Effective relief of pain • Anti-rheumatic function

4. Radix Lysimachia Christinae

Function of the herb: • Counteract toxic effects • To clear up liver heat • Expel urinary stones and promote urination • Tones up liver function • Used for rheumatism

5. Herba Artemisa Scoparia

Function of the herb: • Eliminate damp heat in the liver and gallbladder

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Research by western scientists has shown that this herb is effective for treating hepatitis. The article wrote "Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-gamma/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-gamma/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression." "Scoparone from Artemisia capillaris

inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by

interferon-gamma Plus LPS." Arch Pharm Res. 2005; 28(2):203-8 (ISSN: 0253-6269) Another group of scientists wrote "The hepatoprotective activity of crude extract of artemisia scoparia (aerial parts) was investigated against experimentally produced hepatic damage using carbon tetrachloride (CCl4) as a model hepatotoxin. CCl4 at the dose of 1.5 ml/kg, produced liver damage in rats as manifested by the rise in serum levels of AST and ALT to 395 +/- 110 and 258 +/- 61 IU/l (mean +/- SEM; n = 10) respectively, compared to control values of 106 +/- 15 and 26 +/- 04. Pretreatment of rats with plant extract (150 mg/kg) significantly lowered (P < 0.01), the respective serum GOT and GPT levels to 93 +/- 05 and 27 +/- 03 IU/l, indicating hepatoprotective action. Pentobarbital sodium (75 mg/kg)-induced sleeping time in mice was found to be 140.8 +/- 1.5 min (n = 10) which was similar (P > 0.05) to that obtained in the group of animals pretreated with the plant extract (139.9 +/- 1.8 min). CCl4 treatment extended the pentobarbital sleeping time to 212.2 +/- 19.1 min and pretreatment of animals with plant extract reversed the CCl4-induced prolongation in pentobarbital sleeping time to 143.9 +/- 5.5 min (P < 0.001) which further confirms the protective action of the plant extract against CCl4-induced liver damage. These data indicate that the plant artemisia scoparia is hepatoprotective and validate the folkloric use of this plant in liver damage". "Hepatoprotective effects of artemisia scoparia against carbon tetrachloride: an environmental


scoparia and is known to have potent anti-inflammatory properties. In the current studies, we examined the effects of scoparone on inhibiting lipopolysaccharide (LPS)-induced TF expression in cultured human umbilical vein endothelial cells (HUVECs). Flow-cytometric analysis revealed LPS (10 $g/ml)-activated surface TF induction was concentration-dependently inhibited by scoparone (10-400 $M). The concentrations of scoparone used in this study did not affect cell viability. The elevation of the procoagulant activity of TF by LPS was suppressed by scoparone. The LPS-induced superoxide formation was markedly decreased by scoparone. Messenger RNA expression of TF in LPS-activated HUVECs was also reduced by scoparone. Furthermore, scoparone did not significantly affect the IB degradation. Our results demonstrate that the inhibition of scoparone on LPS-induced TF expression in HUVECs may mediate by the mechanisms suppressing superoxide anion formation and TF transcription. "Scoparone Inhibits Tissue Factor Expression in Lipopolysaccharide-Activated

Human Umbilical Vein Endothelial Cells." Journal of Biomedical Science 2003;10:518-525

(DOI: 10.1159/000072379) "A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris Thunb (Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 microg/mL) inhibited the secretion of EtOH-induced interluekin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). AC also inhibited the EtOH-, IL-1alpha-, and TNF-alpha-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the

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apoptosis of Hep G2 cells." "Inhibitory effect of Artemisia capillaris on ethanol-induced




8):557-63. "Hepatoprotective activity of hydro-methanolic extract of Artemisia scoparia (Compositae) was investigated against acetaminophen-induced hepatic damage. 2. Acetaminophen at a dose of 1 g/kg produced 100% mortality in mice while pretreatment of animals with plant extract (150 mg/kg) reduced the death rate to 20%. 3. Acetaminophen at a dose of 640 mg/kg produced liver damage in rats as manifested by the rise in serum levels of GOT and GPT to 1528 +/- 310 and 904 +/- 261 IU/l (n = 10) respectively, compared to respective control values of 80 +/- 11 and 38 +/- 09. 4. Pretreatment of rats with plant extract (150 mg/kg) lowered significantly the respective serum GOT and GPT levels to 85 +/- 11 and 23 +/- 06. 5. These results indicate that Artemisia scoparia contains hepatoprotective constituents and this study rationalizes the traditional use of this plant in hepatobiliary disorders." "Protective effect of Artemisia scoparia extract against acetaminophen-

induced hepatotoxicity." Gen Pharmacol. 1993 Nov;24(6):1455-8. 6. Caulis Uncaria Rhynchophylla

Function of the herb: • It is used as antipyretic and anticonvulsive agent

7. Folium Taraxacum Mongolicum (Fisherman's Herb) One day, the 16 year old daughter of a government official in ancient China was suffering from mastitis with a triangular lump underneath her left breast. When her maid found out, she asked her father to hire a doctor without telling why. The father thought the daughter had disgraced the family by committing an immoral act. After being punished by the father, she tried to commit suicide by jumping into the river but she was rescued by a fisherman's daughter. When the fisherman found out why, he went to look for a perennial herb, with white milky juice in it, yellowish flowers with straight stems and fleshy thick roots. He boiled some of the herbs for the girl to drink and used some for external application. Finally she recovered and her father named the plant "Fisherman's Herb" Function of the herb:

• Used for inflammatory disease T • To counteract toxic effect • Clear up heat • Disperse swelling • Regulate liver and stomach function


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• Promotes blood circulation • Remove blood stasis • Acts as a tranquilizer

In one study Salvia Miltiorrhiza (Sm) administration has been shown to reduce hepatic fibrosis (improve liver function) in rats. We investigated the hemodynamic effects of Sm on bile duct ligated (BDL) rats. Hemodynamic, histological, and vascular contractile studies were conducted in rats 4 weeks after bile duct ligation. An aqueous extract of Sm (0.2 g twice per day) or vehicle was administered for 4 weeks to BDL rats. Sm treatment in BDL rats significantly reduced histological grades of fibrosis and ameliorated the portal hypertensive state (including portal venous pressure, superior mesenteric artery blood flow, cardiac index, and total peripheral resistance) as compared with vehicle treatment. Moreover, Sm treatment enhanced the vascular sensitivity of mesenteric arteries to phenylephrine in BDL rats. Sm treatment had no effect on plasma biochemical profiles of either BDL or normal rats. Our results suggest that 4-week Sm treatment ameliorates the portal hypertensive state in BDL rats. Article: "Hemodynamic effects of Salvia miltiorrhiza on cirrhotic rats" Canadian

Journal of Physiology and Pharmacology, Volume 79, Number 7, July 2001, pp. 566-572(7) Salvia Miltiorrhiza has also been shown to be able to do the following:

• Activates kallikrein-kinin system in the (rat) kidney to promote production and secretion of prostaglandin E2, inducing dilation of renal vascular system, an increase in renal blood flow

and glomerular filtration rate • Increases coronary blood flow without affecting heart rate


miltiorrhiza on dimethylnitrosamine-intoxicated rats". J Biomed Sci. 2005;12(1):185-95. "Acute liver damage from D-galactosamine was initiated in rats that were then treated with Paeonia lactiflora and Salvia miltiorrhiza. Parameters measured in this study were changes in ALT, bilirubin, levels of plasma fibronectin and pathological histology. The results showed that both herbs increased plasma fibronectin and improved the reticuloendothelial system function. Aggregation of albumin, collagen fragments and immune complexes were markedly reduced. Liver immune damage and micro-circulation disorders were avoided, justifying the important role of Paeonia lactiflora and Salvia miltiorrhiza in protecting hepatocytes".

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Citation: Qi, XG, "Protective mechanism of Salvia miltiorrhiza and Paeonia lactiflora

for experimental liver damage", Chung His Ho Tsa Chih, 11(2), pp 102-4, 1991.


http://www.nutrihuang.com/Uri-X_Testimonials.htm

18) Vira-Liv


1. Hepatitis 2. Hepatomegaly (virus) 3. Liver Cirrhosis (virus) 4. Skin Pigmentation (combined with SpitalCare)

Herbs:

Radix Rehmannia Glutinosa Radix Glycyrrhiza Glabra Herba Artemisiae Scopariae Flos Lonicera Japonica Radix Scutellaria Baicalensis Fructus Zanthii

1. Radix Rehmannia Glutinosa

The Chinese have a famous riddle "I have visited a new place and returned to an old place simultaneously, what herb am I?" Glutious Rehmannia in its raw form is called "new place" and processed Rehmannia is called "old place" (in their Chinese character). Processed Rehmannia is derived from raw Rehmannia by steaming it for ten times and drying in the sun nine times. The functions (clinical usage) of raw and processed Rehmannia are totally different altogether. One herb plus steam and sun gives you two different herbs. That is the magical wonder of herbal science. This knowledge has to be passed down from generation to generation. It cannot be done using laboratory research. It is something that allopathic science cannot explain.

Function of the herb: • Replenish vital essence of the kidneys • Protect the liver

2. Fructus Zanthii


• It is used as an antiallergic, antipruritic and antirheumatic herb

3. Radix Glycyrrhiza Glabra

Function of the herb: • Cleanse and protect the liver • Promotes production of red blood cells • Invigorates the function of the heart and the spleen • Improves the vital energy • Improves the immune system

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Licorice root extract has been used in Japan for more than twenty years as a treatment for chronic hepatitis. Results from randomized clinical trials using licorice root have shown a significant reduction of serum amino-transferase and a significant improvement in liver histology.11,12 11 Van Rossum TG, Vulto AG, de Man RA, Brouwer JT, Schalm SW. Review Article: glycyrrhizin as a potential treatment for chronic hepatitis C. Aliment Pharmacol Ther 1998 Mar; 12(3): 199-205. 12 Yamamura Y, Kotaki H, Tanaka N, Aikawa T, Sawada Y, Iga T. The pharmacokinetics of glycyrrhizin and its restorative effect on hepatic function in patients with chronic hepatitis and chronically carbon-tetrachloride intoxicated rats. Biopharm Drug Dispos 1997 Nov;18(8):717-25. "More than one-third of Americans use herbs for health purposes, yet patients and physicians usually lack accurate information about safety and efficacy of herbal remedies. In recent years, there has been a substantial increase in the use of so-called complementary and alternative therapies by patients with liver disease. Medical professionals and laboratorians need to be informed about popular alternative therapies and be open-minded to the possibility that some benefit may come from some therapies currently regarded as alternative. The active component of licorice root, glycyrrhizin, has been shown to reduce alanine transaminase and aspartate transaminase values in the serum. This protective function has recently been explained as the inhibitory effects of glycyrrhizin on immune-mediated cytotoxicity against hepatocytes and on nuclear factor (NF)-kappa B, which activates genes encoding inflammatory cytokines in the liver." "The use of alternative medicine in the treatment of Hepatitis C" Am Clin Lab. 2002 May;21(4):19-21. "Intravenous administration of glycyrrhizin is known to decrease elevated plasma transaminase levels in patients with chronic viral hepatitis, in which immune-mediated cytotoxicity by cytotoxic T lymphocytes and tumour necrosis factor (TNF)-alpha is considered to play an important pathogenic role. However, the immunological interpretation of the transaminase-lowering action of glycyrrhizin is not known. Studies were performed to elucidate this action immunologically by assessing the effects of glycyrrhizin on immune-mediated cytotoxicity using an antigen-specific murine CD4+ T hybridoma line, which exhibits cytotoxicity against antigen-presenting cells after stimulation with specific antigen, and a murine TNF-alpha-sensitive fibroblast line. Glycyrrhizin inhibited the cytotoxic activity of the T cells against antigen-presenting cells and also suppressed TNF-alpha-induced cytotoxicity in the TNF-alpha-sensitive cell line in vitro. These results suggest that the decrease of elevated transaminase levels by glycyrrhizin in patients with chronic viral hepatitis is mediated in part by inhibition of immune-mediated cytotoxicity against hepatocytes." "Effects of

glycyrrhizin on immune-mediated cytotoxicity." J Gastroenterol Hepatol. 1997 Mar;12(3):243-

8. "Glycyrrhizin, a major component of a herb (licorice), has been widely used to treat chronic hepatitis B in Japan. This substance improves liver function with occasional complete recovery from hepatitis; its effects on the secretion of hepatitis B surface antigen (HBsAg) were examined in vitro. Glycyrrhizin suppressed the secretion of HBsAg and accumulated it dose-dependently in PLC/PRF/5 cells. Its action was further analyzed and determined in the HBsAg-expression system using the varicella-zoster virus. Glycyrrhizin suppressed the secretion of HBsAg, resulting in its accumulation in the cytoplasmic vacuoles in the Golgi apparatus area. HBsAg labeled with 35S-methionine and cysteine accumulated in the cells and its secretion was suppressed dose-dependently in glycyrrhizin-treated culture. The secreted HBsAg was modified by N-linked and O-linked glycans but its sialylation was inhibited dose-dependently by glycyrrhizin. Thus glycyrrhizin suppressed the intracellular transport of HBsAg at the trans-Golgi area after O-linked glycosylation and before its sialylation. HBsAg particles were mainly observed on the cell surface in the glycyrrhizin-treated culture but not in the untreated culture. This suggests that asialylation of HBsAg particles resulted in the novel surface nature of glycyrrhizin-treated HBsAg particles. We elucidated the unique mechanism of action of glycyrrhizin on HBsAg processing, intracellular transport, and secretion." "Effects of glycyrrhizin on hepatitis B surface antigen: a biochemical and morphological study."

J Hepatol. 1994 Oct;21(4):601-9.

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"Glycyrrhizin, an aqueous extract of licorice root, has anti-inflammatory activity and has been used for the treatment of chronic viral hepatitis. In the present study we describe the mechanism by which glycyrrhizin inhibits complement. Glycyrrhizin inhibited the cytolytic activity of complement via the activation of both the classical and alternative pathways, while it had no effect on immune adherence, suggesting that it blocks C5 or a later stage of the complement cascade. Further analysis revealed that glycyrrhizin inhibits the lytic pathway in which the membrane attack complex (MAC) is formed. This mechanism suggests that glycyrrhizin may prevent tissue injury caused by MAC not only in chronic hepatitis but in many autoimmune and inflammatory diseases." "Glycyrrhizin inhibits the lytic

pathway of complement--possible mechanism of its anti-inflammatory effect on liver cells in

viral hepatitis." Microbiol Immunol. 2000;44(9):799-804 "Active compounds in licorice root are also used to help prevent and treat chronic hepatitis (liver inflammation). In one study of Japanese patients with hepatitis C, those who received intravenous treatment with glycyrrhizin, cysteine, and glycine for an average of 10 years were significantly less likely to develop liver cancer and cirrhosis (progressive liver failure) than those who received placebo. In a second study of 57 patients with hepatitis C, glycyrrhizin (in doses ranging from 80 to 240 mg/day) significantly improved liver function after only one month. These effects diminished after glycyrrhizin treatment was discontinued, however." "Arase Y, et al. The long term efficacy of

glycyrrhizin in chronic hepatitis C. Cancer. 1997;79:1494–1500." 4. Flos Lonicera Japonica

Function of the herb: • It is used to protect the liver • It is used to reduce body fat • It is used to counteract toxic effect • It is used to cool down the blood

(This herb contains lonicerin, saponin and inositol and is found to be an effective antibacterial and antiviral herb for the treatment of contagious hepatitis)

5. Radix Scutellaria Baicalensis

Function of the herb: • It is used to protect the liver • It is used to induce liver regeneration. • It is used for the treatment of pyogenic infection. It is used for febrile diseases

Research by western scientists has shown that this herb has anti-inflammatory and anti-cancer

properties. The article wrote "Scutellaria baicalensis is a widely used Chinese herbal medicine that has been used historically in anti-inflammatory and anticancer therapy. The purpose of this study is to verify its anticancer activity on head and neck squamous cell carcinoma (HNSCC) in

vitro and in vivo and to investigate its effect on cyclooxygenase-2 (COX-2), which converts arachidonic acid to prostaglandin E2

(PGE2) and is highly expressed in HNSCC. Two human HNSCC cell lines (SCC-25 and KB) and a nontumorigenic cell line (HaCaT) were tested in vitro for growth inhibition,

proliferation cell nuclear antigen expression, and COX-2 activity and expression after treatment with Scutellaria baicalensis extract. Its effects were compared with those of baicalein (a flavonoid isolated

from Scutellaria baicalensis), indomethacin (a nonselective COX inhibitor), and celecoxib (a selective COX-2 inhibitor). Four nude mice with s.c. inoculation of KB cells were tested for its anticancer activity in vivo by oral administration of

Scutellaria baicalensis at a dose of 1.5 mg/mouse (75 mg/kg), five times/week for 7 weeks. Scutellaria baicalensis and other agents demonstrated a strong growth inhibition in both tested human HNSCC cell lines. No growth inhibition of HaCaT cells was observed with Scutellaria baicalensis. The IC50s were 150

$g/ml for Scutellaria baicalensis, 25 $M for celecoxib, and 75 $M for baicalein and indomethacin. Scutellaria

baicalensis, as well as celecoxib

and indomethacin, but not baicalein, suppressed proliferation cell nuclear antigen expression and PGE2 synthesis in both cell types. Scutellaria baicalensis

inhibited COX-2 expression, whereas celecoxib

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inhibited COX-2 activity directly. A 66% reduction in tumor mass was observed in the nude mice. Scutellaria baicalensis selectively and effectively inhibits cancer cell growth in vitro and in vivo and can be an effective chemotherapeutic agent for HNSCC. Inhibition of PGE2 synthesis via suppression of COX-2 expression may be responsible for its anticancer activity. Differences in biological effects of Scutellaria baicalensis compared with baicalein suggest the synergistic effects among components in Scutellaria baicalensis." Western scientists have proven that this herb can be used to induce liver regeneration. The article wrote "The aim of this study is to elucidate the effects of Scutellaria

baicalensis Georgi (SbG) extract

and its constituents on macrophage-hepatocyte interaction in primary cultures. By using trans-well primary Kupffer cell culture or conditioned medium (CM) from murine macrophage RAW264.7 cell line (RAW cells), effects of SbG on hepatocyte growth were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide and trypan blue exclusion assay. Cytokine production, antibody-neutralization studies, and molecular mechanisms of transforming growth factor (TGF)-ß1 gene expression were elucidated on SbG-treated RAW264.7 cells. In addition, recombinant human TGF-ß1 (r-human TGF-ß1) was added to elucidate the mechanisms of SbG effects on cultured hepatocytes. Immunohistochemistry using anti–NF- B antibody was used to determine the possible signal transduction pathways in primary hepatocyte culture. The results showed that SbG stimulated the proliferation of cultured hepatocytes, possibly through NF- B, but not of Toll-like receptor 4 activation; whereas SbG-RAW-CM and SbG in trans-well significantly suppressed the proliferation of hepatocytes. Antibody-neutralization studies revealed that TGF-ß1 was the main antimitotic cytokine in SbG-treated RAW cells CM. The growth stimulation effect of SbG on cultured hepatocytes was inhibited by exogenous administration of r-human TGF-ß1. Furthermore, SbG induced NF-kB translocation into the nuclei of cultured cells. In the RAW264.7 line, SbG and baicalin stimulated TGF-ß1 gene expression via

NF- B and protein kinase C activation. We conclude that SbG stimulates

hepatocyte growth via activation of the NF- B pathway and induces TGF-ß1 gene expression through the Kupffer cell–hepatocyte interaction, which subsequently results in the inhibition of SbG-stimulated hepatocyte growth."

6. Herba Artemisiae Scoparia

Function of the herb: • It is used to eliminate damp heat in the liver and jaundice caused by hepatitis • It is used to protect the liver • It is used to promote liver bile production and excretion • It is used as an antiviral herb • It is used as an adjuvant herb to treat liver and gall bladder disease

Research by western scientists has shown that this herb is effective for treating hepatitis. The article wrote "Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. Research results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression." "Scoparone from Artemisia

capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation

cells by interferon-gamma Plus LPS." Arch Pharm Res. 2005; 28(2):203-8 (ISSN: 0253-6269) "The hepatoprotective activity of crude extract of artemisia scoparia (aerial parts) was investigated against experimentally produced hepatic damage using carbon tetrachloride (CCl4) as a model hepatotoxin. CCl4 at the dose of 1.5 ml/kg, produced liver damage in rats as manifested by the rise in serum levels of AST and ALT to 395 +/- 110 and 258 +/- 61 IU/l (mean +/- SEM; n = 10) respectively, compared to control values of 106 +/- 15 and 26 +/- 04. Pretreatment of rats with plant extract (150 mg/kg) significantly lowered (P < 0.01), the respective serum GOT and GPT levels to 93 +/- 05 and 27 +/- 03 IU/l, indicating hepatoprotective action. Pentobarbital sodium (75 mg/kg)-induced sleeping time in mice was found to be 140.8 +/- 1.5 min (n = 10) which was similar (P > 0.05) to that obtained in the group of animals pretreated with the plant extract (139.9 +/- 1.8 min).

Page 76

CCl4 treatment extended the pentobarbital sleeping time to 212.2 +/- 19.1 min and pretreatment of animals with plant extract reversed the CCl4-induced prolongation in pentobarbital sleeping time to 143.9 +/- 5.5 min (P < 0.001) which further confirms the protective action of the plant extract against CCl4-induced liver damage. These data indicate that the plant artemisia scoparia is hepatoprotective and validate the folkloric use of this plant in liver damage". "Hepatoprotective effects of artemisia scoparia against carbon tetrachloride: an environmental


scoparia and is known to have potent anti-inflammatory properties. In the current studies, we examined the effects of scoparone on inhibiting lipopolysaccharide (LPS)-induced TF expression in cultured human umbilical vein endothelial cells (HUVECs). Flow-cytometric analysis revealed LPS (10 $g/ml)-activated surface TF induction was concentration-dependently inhibited by scoparone (10-400 $M). The concentrations of scoparone used in this study did not affect cell viability. The elevation of the procoagulant activity of TF by LPS was suppressed by scoparone. The LPS-induced superoxide formation was markedly decreased by scoparone. Messenger RNA expression of TF in LPS-activated HUVECs was also reduced by scoparone. Furthermore, scoparone did not significantly affect the IB degradation. Our results demonstrate that the inhibition of scoparone on LPS-induced TF expression in HUVECs may mediate by the mechanisms suppressing superoxide anion formation and TF transcription." "Scoparone Inhibits Tissue Factor Expression in Lipopolysaccharide-

Activated Human Umbilical Vein Endothelial Cells." Journal of Biomedical Science

2003;10:518-525 (DOI: 10.1159/000072379) "A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris Thunb (Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 microg/mL) inhibited the secretion of EtOH-induced interluekin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). AC also inhibited the EtOH-, IL-1alpha-, and TNF-alpha-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells." "Inhibitory effect of Artemisia capillaris on ethanol-induced




8):557-63. "Hepatoprotective activity of hydro-methanolic extract of Artemisia scoparia (Compositae) was investigated against acetaminophen-induced hepatic damage. 2. Acetaminophen at a dose of 1 g/kg produced 100% mortality in mice while pretreatment of animals with plant extract (150 mg/kg) reduced the death rate to 20%. 3. Acetaminophen at a dose of 640 mg/kg produced liver damage in rats as manifested by the rise in serum levels of GOT and GPT to 1528 +/- 310 and 904 +/- 261 IU/l

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(n = 10) respectively, compared to respective control values of 80 +/- 11 and 38 +/- 09. 4. Pretreatment of rats with plant extract (150 mg/kg) lowered significantly the respective serum GOT and GPT levels to 85 +/- 11 and 23 +/- 06. 5. These results indicate that Artemisia scoparia contains hepatoprotective constituents and this study rationalizes the traditional use of this plant in hepatobiliary disorders." "Protective effect of Artemisia scoparia extract against acetaminophen-

induced hepatotoxicity." Gen Pharmacol. 1993 Nov;24(6):1455-8.


http://www.nutrihuang.com/X-Hepa_Testimonials.htm

19) X-Gas-3


1. Gastric problems 2. Gastric Ulcers 3. Harmonizes and regulates stomach functions 4. Disperses congestion

Herbs:

Herba Agastachis Radix Platycladi Radix Curcuma Amomi Fructus Rhizoma Corydalis Semen Nelumbinis

1. Herba Agastachis (Sister-in-law's Mint)

Function of the herb: • It is used to transform dampness and stimulate the spleen • It is used to harmonize the stomach

They are used internally to improve the appetite and strengthen the digestive system[238]. Considered to be a "warming" herb, it is used in situations where there is "dampness" within the digestive system, resulting in poor digestion and reduced vitality[254]. [238] Bown. D. Encyclopaedia of Herbs and

their Uses. Dorling Kindersley, London. 1995 ISBN 0-7513-020-31. [254] Chevallier. A. The

Encyclopedia of Medicinal Plants Dorling Kindersley. London 1996 ISBN 9-780751-303148

2. Radix Platycladi

Function of the herb: • Used for inflammation • Effective as an expectorant because it contains saponins • Eliminate pus in the respiratory tract

The roots of Platycodon grandiflorum, which belongs to the Campanulaceae family, have been used as a food material and as a traditional Oriental medicine. The effect of P. grandiflorum against lipopolysaccharide (LPS)-stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2 )(PGE(2)) immunoassay, nitric oxide (NO) detection assay, and interleurkin-8 (IL- 8) immunoassay on BV2 microglial cells. The aqueous extract of P. grandiflorum was shown to suppress PGE(2 )synthesis and NO production by inhibiting LPS-stimulated cyclooxygenase (COX)-2 activity and expression of inducible NO synthase (iNOS) mRNAs. In addition, the treatment with P.

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grandiflorum reduced the LPS-induced IL-8 release. These results suggest that P. grandiflorum inhibits PGE(2) and NO production through its suppression of LPS-induced COX-2 and iNOS expression, and also reduces IL-8 secretion by microglial cells. "Effects of Platycodon

grandiflorum on lipopolysaccharide-stimulated production of prostaglandin E2, nitric oxide,

and interleukin-8 in mouse microglial BV2 cells." Source: Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea. J Med Food. 2006; 9(2):169-74 (ISSN: 1096-620X) Previous studies have reported that the saponins isolated from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil saponins (CKS), inhibited cyclooxygenase-2 (COX-2) expression in cultured lipopolysaccharide-activated macrophages. The aim of this presented study was to confirm the anti-inflammatory effects of CKS by examining their effect on the inflammatory response induced by carrageenan in a rat by using an acute air pouch inflammation model. CKS significantly reduced the levels of the inflammatory process markers in the air pouch, such as the volume of exudates, the amount of protein and the number of leukocytes and neutrophils. The levels of TNF-alpha and prostaglandin E2 (PGE2) were also markedly lower in the air pouch of the CKS-treated animals than in the controls. An immunoblot analysis showed that CKS reduced the COX-2 expression level in the exudate cells. In addition, CKS significantly reduced the paw edema induced by carrageenan and also markedly reduced the level of PGE2 production in the inflamed paw. These results suggest that CKS had significant anti-inflammatory effects in vivo. "Inhibitory effect of the saponins derived from

roots of Platycodon grandiflorum on carrageenan-induced inflammation." Source: Department of Pharmacy, College of Pharmacy, Research Center for Proteineous Materials, Chosun University, Kwangju, South Korea. Biosci Biotechnol Biochem. 2006; 70(4):858-64 (ISSN: 0916-8451)

3. Radix Curcuma

Function of the Herb: • to relieve pain • regulating the vital energy in the chest and abdomen area.

“Oral administration to rabbits of water or methanol extracts of the drug significantly decreased gastric secretion and increased the mucin contents of gastric juice. Intragastric administration of an ethanol extract of the drug to rats effectively inhibited gastric secretion and protected the gastroduodenal mucosa against injuries caused by pyloric ligation, hypothermic-restraint stress, indometacin, reserpine, and mercaptamine administration, andcytodestructive agents such as 80%, methanol, 0.6 mol/1 hydrochloric acid, 0.2 mol/1 sodium hydroxide and 25%, sodium chloride. The drug stimulated the production of gastric wall mucus, and it restored non-protein sulfides in rats. Curcumin, one of the anti-inflammatory constituents of the drug, has been shown to prevent and ameliorate experimentally induced gastric lesions in animal models by stimulation of mucin production. However, there are conflicting reports regarding the protective action of curcumin against histamine-induced gastric ulceration in guinea-pigs. The gastric and duodenal anti-ulcer activity of an ethanol extract of Curcuma longa (turmeric) rhizome in rats following oral administration of 500 mg/kg of the extract is described. The extract produced significant anti-ulcerogenic activity in rats. Oral administration of the drug to 116 patients with acid dyspepsia, flatulent dyspepsia, or atonic dyspepsia in a randomized, double-blind study resulted in a statistically significant response in the patients receiving the drug. The patients received 500mg of the powdered drug four times daily for 7 days, Two other clinical trials which measured the effect of the drug on peptic ulcers showed that oral administration of the drug promoted ulcer healing and decreased the abdominal pain involved. Two clinical studies have shown that curcumin is an effective anti- inflammatory drug, A short-term (2 weeks) double-blind, crossover study of 18 patients with rheumatoid arthritis showed that patients receiving either curcumin (1200mg/day) or phenylbutazone (30mg/day) had significant improvement in morning stiffness, walking time and joint swelling: In the second study, the effectiveness of curcumin and phenylbutazone on postoperative inflammation was investigated in a double-blind study. Both drugs produced a better anti-inflammatory response than a placebo, but the degree of inflammation in the patients varied greatly and was not evenly distributed among the three groups.” Alimentary Pharmacology & Therapeutics. 16(10):1689-1699, October 2002.

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The gastric and duodenal anti-ulcer activity of an ethanol extract of Curcuma longa (turmeric) rhizome in rats following oral administration of 500 mg/kg of the extract is described. The extract produced significant anti-ulcerogenic activity in rats. Curcumin and its sodium salt have been showing a strong anti-inflammatory activity in carragenin and caoline-induced edema. Turmeric powder protects the gastric mucosa against irritants. "Review on some plants of Indian traditional

medicine with antioxidant activity." Scartezzini P, Speroni E 2000. R J Ethnoparmacol 71: 23-43. There are several data in the literature indicating a great variety of pharmacological activities of Curcuma longa L. (Zingiberaceae), which exhibit anti-inflammatory, anti-human immunodeficiency virus, anti-bacteria, antioxidant effects and nematocidal activities. Curcumin is a major component in Curcuma longa L., being responsible for its biological actions. Other extracts of this plant has been showing potency too. In vitro, curcumin exhibits anti-parasitic, antispasmodic, anti-inflammatory and gastrointestinal effects; and also inhibits carcinogenesis and cancer growth. In vivo, there are experiments showing the anti-parasitic, anti-inflammatory potency of curcumin and extracts of C. longa. "Nematocidal activity of Turmeric: synergistic action of curcuminoids". Kiuchi F, Goto Y, Sugimoto N, Akao N, Kondo K, Tsuda Y 1993 Chem Pharm Bul 41: 1640-1643. This study was carried out to provide scientific basis for the medicinal use of turmeric (Curcuma

longa) in gastrointestinal and respiratory disorders. The crude extract of turmeric (Cl.Cr), relaxed the spontaneous and K+ (80 mM)-induced contractions in isolated rabbit jejunum as well as shifted the CaCl2 concentration-response curves. In rabbit tracheal preparation, Cl.Cr inhibited carbachol and K+-induced contractions. In anesthetized rats, Cl.Cr produced variable responses on blood pressure with a mixture of weak hypertensive and hypotensive actions. In rabbit aorta, Cl.Cr caused a weak vasoconstrictor and a vasodilator effect on K+ and phenylephrine-induced contractions. In guinea-pig atria, Cl.Cr inhibited spontaneous rate and force of contractions at 14–24 times higher concentrations. Activity directed fractionation revealed that the vasodilator and vasoconstrictor activities are widely distributed in the plant with no clear separation into the polar or non-polar fractions. When used for comparison, both curcumin and verapamil caused similar inhibitory effects in all smooth muscle preparations with relatively more effect against K+-induced contractions and that both were devoid of any vasoconstrictor effect and curcumin had no effect on atria. These data suggest that the inhibitory effects of Cl.Cr are mediated primarily through calcium channel blockade, though additional mechanism cannot be ruled out and this study forms the basis for the traditional use of turmeric in hyperactive states of the gut and airways. Furthermore, curcumin, the main active principle, does not share all effects of turmeric. Source: “Pharmacological basis for the use of turmeric in

gastrointestinal and respiratory disorders”. Life Sciences Volume 76, Issue 26, 13 May 2005, Pages 3089-3105 We investigated the inhibitory mechanism of curcumin and its derivative (CHC007) against #-catenin/T-cell factor (Tcf) signaling in various cancer cell lines. Curcumin is known to inhibit #-catenin/Tcf transcriptional activity in HCT116 cells but not in SW620 cells. To clarify the inhibitory effect of curcumin against #-catenin/Tcf signaling, we tested several cancer cell lines. In addition, in order to verify the inhibitory mechanism, we performed reporter gene assay, Western blot, immunoprecipitation, and electrophoretic mobility shift assay. Since inhibitors downregulated the transcriptional activity of #-catenin/Tcf in HEK293 cells transiently transfected with S33Y mutant #-catenin gene, whose product is not induced to be degraded by adenomatous polyposis coli–Axin–glycogen synthase kinase 3# complex, we concluded that the inhibitory mechanism was related to #-catenin itself or downstream components. Western blot analysis suggested that no change in the amount of cytosolic and membranous #-catenin in a cell occurred; however, nuclear #-catenin and Tcf-4 proteins were markedly reduced by inhibitors and this lead to the diminished association of #-catenin with Tcf-4 and to the reduced binding to the consensus DNA. In the present study, we demonstrate that curcumin and its derivative are excellent inhibitors of #-catenin/Tcf signaling in all tested cancer cell lines and the reduced #-catenin/Tcf transcriptional activity is due to the decreased nuclear #-catenin and Tcf-4. “The inhibitory mechanism of curcumin and its derivative against !-

catenin/Tcf signaling .” FEBS Letters , Volume 579 , Issue 13 , Pages 2965 - 2971

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4. Amomi Fructus

Function of the herb: • it is used to warm up the spleen

Large cardamom (fruit of Amomum subulatum Roxb, N.O. Zingiberaceae) commonly known as ‘Heel kalan’ or ‘Bari Ilaichi’ is used in Unani system of medicine in gastrointestinal disorders. A crude methanolic extract and its different fractions, viz. essential oil, petroleum ether (60–80°), ethyl acetate and methanolic fractions, were studied in rats for their ability to inhibit the gastric lesions induced by aspirin, ethanol and pylorus ligature. In addition their effects on wall mucus, output of gastric acid and pepsin concentration were recorded. The crude methanolic extract of A. subulatum and its fractions, viz. essential oil, petroleum ether and ethyl acetate, inhibited gastric lesions induced by ethanol significantly, but not those which were induced by pylorus ligation and aspirin. However, ethyl acetate fraction increased the wall mucus in pylorus ligated rats. The results suggest a direct protective effect of ethyl acetate fraction on gastric mucosal barrier. While the observation of decrease in gastric motility by essential oil and petroleum ether fractions suggests the gastroprotective action of the test drug. These investigations validate the use of ‘Heel kalan’ in gastrointestinal disorders by Unani physicians. “Evaluation of the gastric antiulcerogenic effect of large cardamom (fruits of

Amomum)”. Journal of Ethnopharmacology Volume 75, Issues 2-3, May 2001, Pages 89-94 To study analgesic and anti-inflammatory effects of bornyl acetate, the main ingredient of Amomum villosum volatile oil. METHODS: The analgesic effects were tested by hot-plate and writhing reaction method, the ear swelling caused by dimethylbenzene in mice. RESULTS: Bornyl acetate could restrain writhing reaction caused by acetic acid glacial, lighten the pain caused by hot-plate. It could also suppress ear swelling caused by dimethylbenzene in mice. CONCLUSION: Bornyl acetate shows analgesic and anti-inflammatory effects. “Studies on the analgesic and anti-inflammatory effect of

bornyl acetate in volatile oil from Amomum villosum.” Zhong Yao Cai. 2004 Jun; 27(6):438-9. In this study, we investigated the effect of Amomum xanthiodes (Zingiberaceae) extract (AXE) on the mast cell–mediated allergy model and studied the possible mechanism of action. We found that AXE inhibited compound 48/80–induced systemic reactions and plasma histamine release in mice. Additionally, AXE decreased immunoglobulin E (IgE)-mediated local allergic reactions and passive cutaneous anaphylaxis (PCA), and AXE dose-dependently attenuated the release of histamine from rat peritoneal mast cells (RPMC) activated by compound 48/80 or IgE. The amounts of AXE needed for inhibition of compound 48/80–induced plasma histamine release and PCA were similar to disodium cromoglycate, the known anti-allergic drug. We found that AXE increased the cAMP levels and decreased the compound 48/80–induced intracellular Ca2+. Furthermore, AXE attenuated the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (A23187)–stimulated tumor necrosis factor- (TNF- ) and interleukin (IL)-6 secretion in human mast cells. The inhibitory effect of AXE on the proinflammatory cytokines was nuclear factor- B (NF- B)–dependent. In addition, AXE decreased PMA plus A23187–induced degradation of I B and the nuclear translocation of NF- B. Our findings provide evidence that AXE inhibits mast cell–derived immediate-type allergic reactions, and that cAMP, intracellular Ca2+, proinflammatory cytokines, and NF- B are involved in these effects. “Amomum xanthiodes Inhibits Mast Cell–Mediated Allergic Reactions Through the Inhibition

of Histamine Release and Inflammatory Cytokine Production (treats gastric problems)” Experimental Biology and Medicine 230:681-687 (2005) © 2005 Society for Experimental Biology and Medicine

5. Rhizoma Corydalis

Function of the herb: • relieve pain by invigorating blood circulation • regulating the vital energy in the chest and abdomen area.

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Scientists have isolated a number of alkaloids from the tuber of corydalis, including corydaline, tetrahydropalmatine (THP), dl-Tetrahydropalmatine (dl-THP), protopine, tetrahydrocoptisine, tetrahydrocolumbamine, and corybulbine.3 Of the full range of 20 alkaloids found in the plant, THP is considered to be the most potent. In laboratory research, it has been shown to exhibit a wide number of pharmacological actions on the central nervous system, including analgesic and sedative effects.4

(3. Hsu HY. Oriental Materia Medica: A Concise Guide. Long Beach, CA: Oriental Healing Arts

Institute, 1986, 448–50. 4. Zhu YP. Chinese Materia Media: Chemistry, Pharmacology, and

Applications. Australia: Harwood Academic Publishers, 1998, 445–8.) Extracts of the herb may also be useful in the treatment of stomach ulcers. In a large sample of patients with stomach and intestinal ulcers or chronic inflammation of the stomach lining, a 90–120 mg extract of the herb per day (equal to 5–10 grams of the crude herb) was found to improve healing and symptoms in 76% of the patients. Source: Chang HM, But PPH. Pharmacology and Applications

of Chinese Materia Medica vol 1. Singapore: World Scientific Inc., 1986, 521.

6. Semen Nelumbinis

Function of the herb: • to consolidate vital energy


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20) X-Kolest


I. High blood pressure 2. High cholesterol 3. Rejuvenates blood vessels (arteriosclerosis) 4. Breathlessness 5. Cold hands and feet, numbness, Circulation problems 6. Protects cardiac muscle from ischemic injury (strengthens the heart) 7. Increases red blood cells production 8. Cancer inhibitive functions 9. Anti-tumor effect 10. Anti-fatigue 1I. Improves immunity 12. Reduces blood fats 13. Antioxidant (protects body against free radicals) 14. Improves brain function Herbs:

Radix Polygonum Multiflorum Radix Saposhnikovia Divaricata Radix Salvia Miltiorrhiza Fructus Schisandra Chinensis 1. Radix Polygonum Multiflorum


• To tone up liver and kidney and replenish their vital essence • Treat fatty liver, increase red blood cells and reduce blood fats • Exert same effect as adrenoticortical hormone • Breathlessness • Protect heart from damage from free radicals • Anti aging • Improve immune system through its antioxidant effect • Treat cholesterol problem • Retard and treat arteriosclerosis (hardening of blood vessels) • Regulate and nourish blood • Increase red blood cell production

Polygonum Multiflorum: Research shows that "The extract of the root of Polygonum multiflorum exhibited a significant antioxidant activity assessed by the DPPH radical scavenging activity in vitro. The bioassay-guided fractionation of the extract yielded a stilbene glucoside, (E)-2,3,5,4'-

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tetrahydroxystilbene-2-O-beta-d-glucopyranoside (1) as an active constituent responsible for the antioxidant property. Compound 1 demonstrated a moderate DPPH radical scavenging activity (IC50, 40 microM), while the corresponding deglucosylated stilbene 2 exhibited a much higher activity" (IC50, 0.38 microM). “The radical scavenging effects of stilbene glucosides from Polygonum

multiflorum” Arch Pharm Res. 2002 Oct;25(5):636-9.

2. Radix Saposhnikovia Divaricata


• Use as diaphoretic for affection due to wind and clod and rheumatic pain. • To relieve pain

Cimifugin, one of the analgesic and anti-hypertension principle component of Fang-Feng, was isolated from the root of Saposhnikovia divaricata. Reversed-phase high-performance liquid chromatography was employed to determine the contents of cimifugin in Fang-Feng. The separation was performed on a LiChrospher 100 RP-18e column (4 mm I.D.¡Ñ250 mm) by isocretic elution with acetonitrile-water (v/v) (18:82) as the mobile phase at a flow-rate of 1.0 ml/min, the temperature keep at 40¢J and with detection at 220 nm. Methylparaben was used as the internal standard and a regression equation revealed linear relationship between the peak-area ratio (marker substance/internal standard) and concentration. We found that the decoction from Fang-Feng with 20 times of 70% aqueous methanol in 40 minutes of sonication given the best yield of cimifugin. This HPLC method can rapidly and simply quantify the cimifugin present in Fang-Feng and can be used for quality control of commercial crude drug of Fang-Feng. “The Marker Standard Preparation of

Saposhnikoviae Radix and Quality Control” Institute of Pharmacognosy Science Taipei Medical

College CCMP87-RD-029



• Promote blood circulation • Treat coronary heart disease • Increase red and white blood cells • Protect liver • Improve function of liver and spleen

In one study Salvia Miltiorrhiza (Sm) administration has been shown to reduce hepatic fibrosis (improve liver function) in rats. We investigated the hemodynamic effects of Sm on bile duct ligated (BDL) rats. Hemodynamic, histological, and vascular contractile studies were conducted in rats 4 weeks after bile duct ligation. An aqueous extract of Sm (0.2 g twice per day) or vehicle was administered for 4 weeks to BDL rats. Sm treatment in BDL rats significantly reduced histological grades of fibrosis and ameliorated the portal hypertensive state (including portal venous pressure, superior mesenteric artery blood flow, cardiac index, and total peripheral resistance) as compared with vehicle treatment. Moreover, Sm treatment enhanced the vascular sensitivity of mesenteric arteries to phenylephrine in BDL rats. Sm treatment had no effect on plasma biochemical profiles of either BDL or normal rats. Our results suggest that 4-week Sm treatment ameliorates the portal hypertensive state in BDL rats. Article: "Hemodynamic effects of Salvia miltiorrhiza on cirrhotic rats". Canadian

Journal of Physiology and Pharmacology. Volume 79, Number 7, July 2001, pp. 566-572(7) Salvia Miltiorrhiza has also been shown to be able to do the following:

• Activates kallikrein-kinin system in the (rat) kidney to promote production and secretion of prostaglandin E2, inducing dilation of renal vascular system, an increase in renal blood flow and glomerular filtration rate

• Increases coronary blood flow without affecting heart rate

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miltiorrhiza on dimethylnitrosamine-intoxicated rats". J. Biomed Sci. 2005;12(1):185-95.



11(2), pp 102-4, 1991.




4. Fructus Schisandra Chinensis


• Effective heart tonic • Used to treat liver and gall bladder stones • Used to treat insomnia and forgetfulness • Used to treat for neurasthenia (tiredness and exhaustion)

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• Researchers now recognize it is an “adaptogen” herb – capable of increasing the body’s

resistance to disease, stress and other debilitating process, boosts recovery after surgery and improves health of adrenals.

• It is used as an adjuvant herb to treat liver disease Schisandra Chinensis: “Schisandra chinensis (TURCZ.) BAILL., originally a Japanese-Manchurian endemite, yields a vegetable drug (Schisandrae fructus) with a number of very utilizable therapeutic effects. The paper reports the results of phytochemical and pharmacological-toxicological studies approximately from the year 1990 carried out both with the drug and, in particular, the principal isolated lignans of the dibenzo[a,c]cyclooctadiene type. The results confirm the validity of the historical use of the drug, in particular as a hepatoprotective, adaptogenic, and antioxidative agent. It is obvious that a very positive therapeutic effect based on the use of a complex mixture of its principal constituents because their biological effects are complementary and potentiate each other. At the same time, some lignans (e.g. gomisin A, gomisin N) are interesting as new prospective medicines” “Phytotherapeutic aspects of diseases of the circulatory system. 7. Schisandra chinensis (Turcz.)

Baill.): its composition and biological activity”. Ceska Slov Farm. 2001 Jul;50(4):173-80. Schisandra Chinensis: "The effects of Schisandrin B (Sch B) and dimethyl-4,4'-dimethoxy-5,6,5',6'dimethylene-dioxy-biphenyl-2,2'-+ ++bicarboxylate (DDB) treatment on myocardial ischemia-reperfusion (IR) injury in isolated perfused rat hearts were examined under both in vitro and ex vivo conditions. In vitro administration of liposome-entrapped Sch B or DDB during reperfusion did not protect against myocardial IR injury, whereas ascorbic acid or Trolox supplemented perfusate produced protective effect, as evidenced by the significant decrease in the extent of lactate dehydrogenase leakage as well as an improvement in contractile force recovery. Myocardial protection afforded by N-acetyl-L-cysteine supplemented perfusate was not accompanied by the enhancement in contractile force recovery. In ex vivo experiment, pretreatment of Sch B (0.6/1.2 mmol/kg/day x 3) protected against IR-induced myocardial damage in a dose-dependent manner. The myocardial protection was associated with an enhancement in myocardial glutathione antioxidant status, as indicated by significant reductions in both the extent of IR-induced reduced glutathione depletion and inhibition of Se-glutathione peroxidase and glutathione reductase activities. In contrast, the inability of DDB pretreatment to enhance myocardial glutathione antioxidant status resulted in a failure in preventing IR injury. The ensemble of results suggests that the myocardial protection afforded by Sch B pretreatment, which was unlikely due to free radical scavenging action, may be mainly mediated by the enhancement of myocardial glutathione antioxidant status, particularly under oxidative stress conditions." “Schisandrin B protects against myocardial ischemia-reperfusion

injury by enhancing myocardial glutathione antioxidant status.” Mol Cell Biochem. 1999 Jun;

196(1-2):151-6.


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21) P 21


1. Acne 2. Ant Bites 3. Bee Stings 4. Bed Sores 5. Blocked Nose 6. Burns 7. Cold Sores 8. Diabetic Ulcer 9. Folliculitis 10. Genital Rashes 11. Gingivitis 12. Glossitis 13. Infected Ear 14. Infected Wound 15. Leukorrhoea 16. Mouth Ulcer 17. Paronychia (An infection that develops along the edge of the fingernail or toenail) 18. Sinusitis 19. Skin Rashes 20. Sore Throat 21. Toothaches

Herbs:

Radix Rheum Palmatum Semen Prunus Persica Commiphora Myrrha Rhizoma Zingiberis Radix Angelica Dahurica Rice wine

Overall Treatment Pathology

A good tincture must have the following holistic properties: • Analgesic: it relieves the pain

• Anti-inflammatory: reduce swelling of the wound

• Anti-histaminic: control the release of histamines within the skin

• Anti-exudates: prevent the production of skin fluid to allow the wound to dry faster • Antipruritic properties: controls / stop itch

• Antibiotic: cleanse the skin of harmful bacteria and virus

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• Anti-toxin: it neutralizes poison produced by the decaying tissue , bacteria or virus • A skin surface cleanser • Promotes micro blood circulation to bring efficient cell and tissue regeneration to quickly dry up

the wound

P 21 is a traditional Chinese herbal medicine (Tincture). The Tincture is formulated using herbs soaked in rice wine. The herbs are Angelica Dahurica, Rheum Palmatum, Radix Zingiberis, Semen Prunus Persica, Acanthopanax Gracilisstylus and Commiphora Myrrha. It is nontoxic and can be used for internal and external applications by both young and old. How effective is P 21? Let’s take an example the problems with burns. For burns (second or third degree), the skin would normally blisters and fluid (exudates) would collect beneath the skin with half an hour after the accident. Normally, burns take one to two weeks to recover if normal medicine is applied. However if one applies P 21 within 30 to 60 seconds after the accident for about 6 to 7 times at 30 second interval, neither blisters nor fluid would form. The affected skin would feel as if the burn has not taken place at all. There would not be any blisters or burn mark. P 21 does not stain the skin and has no side-effects and is normally applied two to three times a day for the above 21 problems

A typical proper diabetic ulcer healing sequence from P 21

Prevention using herbal capsules products:

Another Success from P 21!! This

person had 3 non-healing diabetic

wound ulcers after operation.

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The best and most effective way to treat diabetic ulcers is to prevent it in the first place. Using chemical methods to lower blood sugar is a reason why diabetic patients develop: 1) Neuropathy 2) Retinopathy, 3) Poor circulation, 4) Other diabetic complications like organ damage by drugs The key to prevent the development of diabetic ulcers problems is to use a holistic approach to treat the disease: 1) Lower and control sugar levels 2) Nourish nerves and blood vessels 3) Strengthen kidney, liver, pancreas, spleen and bladder 4) Improve blood circulation An ounce of prevention is worth a pound of cure


http://www.nutrihuang.com/Potion%2020_DiabeticSores.htm

22) D T J Traditionally used for symptomatic relief of problems related to:

1. Sprain 2. Compressed Nerves Injury 3. Nerve Injuries

Herbs:

Rehmania Glutinosa Semen Prunus Persica Cortex Acanthopanax Gracilistylus Ramulus Cinnamonium Cassia Rhizoma Notopterygii Radix Rheum Palmatum Rice Wine

1. Rhemannia Glutinosa

Function of the herb: • Used to nourish the vital essence for the treatment of its deficiency • It helps strengthen the bones and tissue

Research have shown that Rehmannia Glutinosa can helps strengthen the bones and tissue. It stimulates the formation of red blood cells and other immune potentiating substances in the bone marrow. It stimulate the secretion of another important kidney hormone, erythropoietin. This hormone acts on the bone marrow to stimulate the proliferation of precursor cells and their maturation into erythrocytes. 2. Semen Prunus Persica

Function of the herb: • Promote circulation • For traumatic injuries

3. Cortex Acanthopanax Gracilistylus

Function of the herb: • Anti-rheumatic and effective for relieving pain • Diuretic for the treatment of edema and oliguria • It produces adrenocortical hormones

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Acanthopanax Gracilistylus can be used for relieving pain and enhances the production of adrenocortical hormones. Adrenocortical hormones are any of the various hormones secreted by the adrenal cortex, especially cortisol, aldosterone, and corticosterone. It is also called cortical hormone. Cortisol helps to reduce neurogenic stress and injuries. Cortisol is clinically used in the form of hydrocortisone or prednisone to help control acute inflammation. Unfortunately cortisol type drugs do not correct the basic disease condition. With prolonged use over weeks and months it will also cause many of the above described side effects such as muscle weakness, weakness of the immune system and edemic swelling of the tissues. But plant based cortisol type chemicals like allantoin and our body's cortisol hormones do not have such problems. In fact plant based cortisol type chemical and our body's cortisol helps to increase the transport of amino acids from the extracellular fluids by helping enzymes to convert amino acids into blood glucose. Because cortisol mobilizes amino acids, it is effective in helping to repair damaged tissue. Aldosterone helps to maintain and control the biochemical balances e.g. potassium and salt balances in the body. Aldosterone, the most important mineralocorticoid is mainly controled by the renin-angiotensin mechanism and by the concentration of potassium in the extracellular fluid. In the biochemical balances goes out of sync it can create havoc in the body. For example an excessive loss of potassium from the extracellular fluid can lead to muscular weaknesses.

Acanthopanax Gracilistylus: “To study the effect of Acanthopanax Gracilistylus var pubescens LI saponins (AGVPS) on human platelet aggregation and platelet factor 4 (PF4) liberation in vitro. RESULTS: AGVPS inhibited in vitro the ARmax with IC50 of 1.33 (95% confidence limits: 1.09-1.63, ADP-induced), 1.66 (1.54-1.79, adrenaline-induced), and 4.2 g.L-1 (0.6-29, collagen-induced). The DR5 min (on ADP-induced aggregation) and LT (collagen-induced) were also increased as well. Meanwhile, AGVPS 0.63-2.50 g.L-1 prolonged HTCT on ADP- and collagen-stimulated PF4 liberation. At 0.34-1.39 g.L-1, AGVPS reduced the wet and dry weight of thrombi formed in vitro. CONCLUSION: AGVPS inhibits human platelet aggregation, liberation, and thrombosis in vitro, suggesting its possible antithrombotic action in man.” “Inhibitory effects of Acanthopanax

Gracilistylus saponins on human plate aggregation and platelet factor 4 liberation in vitro”

Chen XC, Xia L, Hu S, Huang G Department of Pharmacology, Hainan Medical College,

Haikou, China. PMID 9863147 4. Ramulus Cinnamonium Cassia

Function of the herb: • To promote blood circulation

5. Rhizoma Notopterygii

Function of the herb: • Used as an analgesic agent

Notopterygium Incisum: Clinical research by western scientists have shown that notopterol, the

analgesic component of Notopterygium incisum, has anti-inflammatory activity by its inhibitory

effect in the vascular permeability test. 39339 "Notopterol was identified as the analgesic component of Notopterygium incisum by using the acetic acid-induced writhing method. Notopterol also indicated an anti-inflammatory activity by its inhibitory effect in the vascular permeability test. The intensive prolongation of pentobarbital-induced hypnosis was possibly caused by its inhibitory effect on the drug metabolism in liver. Pharmacological differences between the analgesic components of N. incisum, Aralia cordata and Angelica pubescens were also discussed." "Analgesic

component of Notopterygium incisum Ting" Okuyama E Nishimura S Ohmori S Ozaki Y

Satake M Yamazaki M, Faculty of Pharmaceutical Sciences, Chiba University, Japan Chem

Pharm Bull (Tokyo). 1993 May;41(5):926-9 ISSN: 0009-2363 PMID: 8339339 6. Radix Rheum Palmatum

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Function of the herb: • Treatment of amenorrhoea due to blood stasis, hematemesis and epistasis • It activates the blood, remove blood coagulation and promotes energy flow.


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23) ErecKing Tonic


1. Improve and enhance men’s vitality 2. Erectile Dysfunction 3. Strengthen bodily functions 4. Improve men’s “appetite” 5. Remove tiredness after sexual activity Time to see results:

Varies from man to man. Usually within 4 to 7 days. Very rarely that it takes more than 10 day.

Initial restoration effect:

Body feels very warm, feels energized, alert, feel refreshed and improved digestion and bowel movement Herbs:

1) Fructus Rubi 2) Radix Morinda Officinalis 3) Radix Achyranthes Bidentatae 4) Radix Rehmannia Glutinosa 5) Fructus Cornus Officinalis 6) Semen Cuscutae 7) Herba Cistanche Deserticola 8) Flos Carthami 9) Herba Epimedii 10) Rhizoma Ligusticus Chuanxiong 11) Radix Dipsacus Asper 12) Radix Paeonia Lactiflora 13) Radix Salvia Miltiorrhiza 14) Radix Angelica Sinensis Rice wine make up the rest Overall Treatment Pathology

Herbal Science: To treat ED problems herbal science would use herbs to rejuvenate, nourish and strengthen the function of the body and its organs so that the root problem would be treated. Herbs can be used to strengthen the kidneys and liver, to nourish the blood and relax the smooth muscles. (That is what Viagra does. The synthetic chemical relaxes the smooth muscles to cause an

erection but with the added side effects). This is TCM's way to of restoring the body to better health and vitality. All these herbs used by herbal scientists have been proven to improve the

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functions of the body without any side effects or causing seriously long-term damage to the body unlike allopathic drugs. TCM knows that kidneys control bone growth. For bone to grow, the body needs bone marrow. In TCM bone marrow is derived from kidney essence. That explains why those who suffer from osteoporosis also suffer from dysfunction kidneys. In recent years, many bone disorders have been successfully treated by TCM practitioners through treatment of the kidney. The above researches have shown that these herbs improve the functions of the kidneys, liver, control and reduce cholesterol, improve blood essence and relax the smooth muscles. They work synergistically to restart the men’s engine to make life more meaningful. It has long lasting rejuvenation effect compared to Viagra which only relaxes the smooth muscles and can give headache and other side effects. 1. Fructus Rubi


• Used for treatment of frequent urination. • Preserve Essence

2. Radix Morinda Officinalis

Function of the herb: Ÿ It is used to reinforce the vital function of the kidneys especially that of sexual organs

for the treatment of impotence and premature ejaculation of men.

a) It is used to reinforce the vital function of the kidneys especially that of sexual organs for the treatment of impotence and premature ejaculation of men. The herb is pungent and sweet in flavour, slightly warm in nature, it is tropistic to the liver and kidney channels. Being sweet and warm, it can invigorate kidney-yang; as a pungent and warm agent, it is capable of dispelling wind-dampness. Being slightly warm, moist and dry, it has effects of invigorating Yang and supplementing vital essence and is especially effective in strengthening muscles and bones, serving to treat insufficiency of kidney-yang and deficiency-cold of essence and blood marked by impotence, infertility and athralgia due to wind-dampness. It tonifies the kidney, invigorating Yang, strengthening muscles and bones, dispelling wind and eliminating dampness. Source: "Legendary Chinese Healing Herbs" -

Dr. Henry Lu- Academy of Oriental Heritage, Blaine, USA. The author is best known for his

translation of "Yellow Emperor's Classics of Internal Medicine" written around 300 B.C. from

Chinese to English; "Dictionary Of Traditional Chinese Medicine" written by Prof. Lou Zhicen

- Professor of Pharmacognosy, Prof. Li Shuncheng - Associate Professor of Medicine and Prof.

Tang Zijin - Associate Professor of Medicine of Beijing Medical College.

b) Research showed that it benefits the reproductive organs. A 30g/kg/day dose of Radix Morindae Officinalis decoction was administered to castrated mice once a day for 15 days. Results showed that the weights of levator ani muscles, seminal vesicle and prostate were not significantly effected. Qiao Zhisheng, et al. Comparison of pharmacological activities of different Radix

Morindae Officinalis species, Chinese Journal of Integrated Traditional and Western Medicine.

1991; 11(7):4

3. Radix Achyranthis Bidentatae

Function of the herb: • It is used to nourish the liver and kidneys Researches by scientists have shown that Radix Achyrantes can be used to improve bone quality. Bones are forms from bone marrows. Bone marrows come from kidney essence. One of the reasons

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why men suffer from ED problems is because of weakening kidneys. One of the phyto-chemicals of Achyrantes is beta-sitosterol. Researches by scientists have discovered that beta-sitosterol reduces blood levels of cholesterol, and is sometimes used in treating hypercholesterolemia (high level of cholesterol in the blood. It is a well known fact high cholesterol can cause ED problems. Beta-sitosterol also prevents the oxidation of LDL cholesterol thereby reducing the risk of atherosclerosis. a) TCM believes that kidneys control bone growth. For bone to grow, the body needs bone

marrow. In TCM bone marrow is derived from kidney essence. That explains why those who

suffers from osteoporosis also suffers from dysfunction kidneys. In recent years, many bone

disorders have been successfully treated by TCM practitioners through treatment of the kidney.

Bioassay-directed fractionation of a butanol-soluble fraction of methanol extract of the root of Achyranthes bidentata resulted in the isolation of 5 new oleanolic acid glycosides 1-5, namely, 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester (1), achyranthoside C dimethyl ester (2), achyranthoside C butyl dimethyl ester (3), achyranthoside E dimethyl ester (4), and achyranthoside E butyl methyl ester (5), together with 10 known compounds. Their structures were established on the basis of spectroscopic interpretation and chemical methods. All the oleanolic acid glycosides inhibited the formation of osteoclast-like multinucleated cells (OCLs) induced by 1alpha,25(OH)2D3 in a co-culture assay system. (An osteoclast (from the Greek words for "bone" and "broken") is a type of bone cell that removes bone tissue by removing the bone's mineralized matrix. This process is known as bone resorption. Osteoclasts and osteoblasts are instrumental in controlling the amount of bone tissue. Osteoblasts form bone; osteoclasts resorb bone. Osteoclasts are formed by the fusion of cells of the monocyte cell line. Osteoclasts are characterized by high expression of tartrate resistant acid phosphatase (TRAP) and cathepsin K.). "Five new oleanolic acid glycosides from Achyranthes

Bidentata with inhibitory activity on osteoclast formation." Planta Med 2005 July 71 (7): 673-9

b) Eight compounds were separated from the roots of Achyrathes bidentata by repeated chromatography. On the basis of physicochemical properties and spectral analysis their structures were elucidated as alpha-spinasterol (1), beta-sitosterol (2), chrysophanol (3), dibutyl phthalate (4), palmitic acid (5), alpha-spinasterol-3-O-beta-D-glucoside (6), daucosterol (7) and ecdysterone (8). Compounds 1-7 were isolated from the plant for the first time. “Separation and identification of the

compounds from Achyranthes bidentata Bl” Zhongguo Zhong Yao Za Zhi 1997

May;22(5):293-5, 319-20.

c) Beta-sitosterol (BS) is a compound that has shown various activities potentially useful for human health. In the present study, we determined its antigenotoxic capacity and lymphocyte induction potential in mouse as well as its capacity to trap free radicals in vitro. BS, in doses from 200 to 1,000 mg/kg, was able to significantly reduce the frequency of sister chromatid exchanges induced by 10 mg/kg of doxorubicin (DX) in bone marrow cells. The same range of BS doses also gave rise to a strong reduction in the rate of micronucleated, polychromatic erythrocytes induced by DX. In addition, we determined an increase in the production of lymphocytes in mice administered with BS. By means of the DPPH assay, the compound was shown to trap free radicals in a concentration dependent manner as high as 78.12% using 250 µg/ml. Our research established three relevant biological activities of BS which show its potential as a chemopreventive (The use of chemical agents, drugs, or food supplements to prevent disease) agent. “Cell protection induced by beta-

sitosterol: inhibition of genotoxic damage, stimulation of lymphocyte production, and

determination of its antioxidant capacity”. Archives of Toxicology DOI 10.1007/s00204-

007-0277-3

d) Research by scientists showed that beta-sitosterol decreases cholesterol synthesis. CaCo-2 cells were used to address the effect of the plant sterol, beta-sitosterol, on cholesterol trafficking, cholesterol metabolism, and apoB secretion. Compared to cells incubated with micelles (5mM taurocholate and 250 microM oleic acid) containing cholesterol, which caused an increase in the influx of plasma membrane cholesterol to the endoplasmic reticulum and increased the secretion of cholesteryl esters derived from the plasma membrane, beta-sitosterol did not alter cholesterol

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trafficking or cholesteryl ester secretion. Including beta-sitosterol in the micelle together with cholesterol attentuated the influx of plasma membrane cholesterol and prevented the secretion of cholesteryl esters derived from the plasma membrane. Stigmasterol and campesterol had effects similar to beta-sitosterol although campesterol did not promote a modest influx of plasma membrane cholesterol. Including beta-sitosterol in the micelle with cholesterol decreased the uptake of cholesterol.Compared to cholesterol, 60% less beta-sitosterol was taken up by CaCo-2 cells. No observable esterification of beta-sitosterol was appreciated and the transport of the plant sterol to the basolateral medium was negligible. Cholesterol synthesis and HMG-CoA reductase activities were decreased in cells incubated with beta-sitosterol. This was associated with a decrease in reductase mass and mRNA levels. Cholesteryl ester synthesis and ACAT activities were unaltered by beta-sitosterol. Both stigmasterol and campesterol decreased reductase activity, but only campesterol increased ACAT activity. Beta-sitosterol did not affect the secretion of apoB mass. The results suggest that beta-sitosterol does not promote cholesterol trafficking from the plasma membrane to the endoplasmic reticulum. Beta-sitosterol interferes with the uptake of micellar cholesterol causing less plasma membrane cholesterol to influx and less cholesteryl ester to be secreted. Despite its lack of effect on cholesterol trafficking, beta-sitosterol decreases cholesterol synthesis at the level of HMG-CoA reductase gene expression. "Effect of micellar beta-sitosterol on cholesterol metabolism in

CaCo-2 cells." Journal of Lipid Research, Vol 38, 348-360

e) Some of the researches done on the relationship between beta-sitosterol and cholesterol: i) At McGill University in Montreal (Can. J. Physiol. Pharmacol. 75, 1997, p. 217-27) doctors did a review of the literature on beta-sitosterol and cholesterol metabolism. They researched in detail 18 major studies that used sitosterols to lower cholesterol and triglycerides. They concluded, "addition to diet of phytosterols represents an effective means of improving circulating lipid profiles to reduce risk of coronary heart disease." This study came complete with forty high quality references and left no doubt about the effectiveness of phytosterols on humans. Also at McGill University (Metabolism Clinic Experiments 47, 1998, p. 751-6) patients on a fixed diet were given sterols from pine oil for a mere ten days in a strict, randomized crossover study. This was not a low fat or low cholesterol diet at all. They successfully lowered both their total cholesterol and LDL levels in this short term placebo controlled experiment. They concluded, these results demonstrate the short term efficacy of pine oil plant sterols as cholesterol lowering agents" ii) A very interesting study was done at the Center for Human Nutrition in France (Ann. Nutr. Metab. 39, 1995, p. 291-5) in that healthy people with normal cholesterol levels were given beta- sitosterol to see if their normal levels could be lowered even further. We always think of studies as using unhealthy people with pathological cholesterol levels given supplements to make them normal again. Amazingly enough the healthy people lowered their normal cholesterol levels even more with no change in diet or exercise. In fact, they were a full 10% lower in only a month. This kind of effect is really fascinating. They said, "The present results may be of great interest in the prevention of high cholesterol diet-associated risks, especially in the prevention of cardiovascular diseases. Since beta-sitosterol was so effective for people who didn't even need it, think what it will do for those people who do need to lower their blood lipids. They concluded, "These findings suggest that a significant lowering of plasma total and LDL cholesterol can be effected by a modest dietary intake of soybean phytosterols" iii) A good study was done at the Wageningen Agricultural Institute in the Netherlands, the same clinic that did so much good research on trans fatty acids (Am. J. Clin. Nutr. 72, 2000, p. 1510-5). They gave men and women a margarine containing plant sterols and got very significant reductions in cholesterol as well as lower LDL levels in only three weeks. Why a clinic would give margarine to people after studying the negative effects of hydrogenated oils is another matter. Again, these were healthy subjects with normal cholesterol levels, yet they still got great benefits very quickly with no change in diet or exercise.

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iv) At Uppsala University in Sweden (Eur. Heart J. Supp. 1, 1999, p. S80-S90) the doctors wanted to give the volunteers the phytosterols in conjunction with a cholesterol lowering diet to see the results of a more comprehensive lifestyle program. The results were really impressive in that the men and women lowered total cholesterol a full 15% and LDL cholesterol a full 19% in less than a month. The shows the very dramatic results you can get with just adding some reasonable dietary changes even without any exercise program at all. v) At the University of British Columbia at their St. Paul's Hospital (American Journal of Medicine 107 (1999) p. 588-94) a very impressive review was done complete with 86 references of using plant sterols to lower total cholesterol and LDL. They said of the recent research, "In 16 recently published human studies that used phytosterols to decrease plasma cholesterol levels in a total of 590 subjects, phytosterol therapy was accompanied by an average 10% decrease in total cholesterol and 13% decrease in LDL cholesterol levels." This is the best review to date and should convince anyone of the effectiveness of sterols over drugs. vi) At the University of Kagawa in Tokyo two studies were done. The first was done on healthy young men who were given plant sterols for only five days. In this short time their cholesterol levels fell measurably (Joshi Eiyo Daigaku Kiyo 14, 1983, p. 165-72). The second study was done on healthy young women (same journal 15, 1984 p. 11-18) again giving them plant sterols for only five days. "Administration of phytosterol (mainly sitosterol) increased the output of fecal cholesterol." These were all healthy young Japanese people eating a traditional low-fat diet who did not have a cholesterol problem to begin with, yet they received measurable results in only five days. vii) At the University of California in San Diego men were isolated in a hospital ward and fed 350 mg of cholesterol and then beta-sitosterol supplements (American Journal of Clinical Nutrition 35, 1982, p. 697-700). This resulted in a 42% decrease in cholesterol absorption in the intestines. They said, "Evidently, the judicious addition of beta-sitosterol to meals containing cholesterol rich foods will result in a decrease in cholesterol absorption with a consequent decrease in plasma cholesterol" viii) The University of Helsinki took a big interest in lowering cholesterol with plant sterol therapy back in 1988. The first study (Clinical Chimica Acta 178, p. 41-9) studied familial (genetic) hypercholesteremia. The higher the sterol levels they found in the patients blood the more cholesterol was excreted rather than absorbed. The second study was in 1989 (Metabolism Clinical Experiments 38, p. 136-40). Men were studied again for blood levels of sterols and they found the higher the levels the more cholesterol was excreted successfully. The third study in 1994 (American Journal of Clinical Nutrition 59, p. 1338-46) studied vegetarians who eat twice as many plant sterols as normal people. They showed one reason vegetarians have lower cholesterol levels besides the food they eat is the efficiency of their cholesterol excretion due to their intakes of plant sterols. In the last study in 1999 (Current Opinion Lipidology 10, p. 9-14) they said, "Plant sterols may be useful for the treatment of hyper-cholesteremiaãƒ•hey may have a potent cholesterol lowering effect as shown in normal and hypercholesteremic men and women with and without coronary heart disease and diabetes mellitus" ix) The best study of all was a review from the University of British Columbia (American Journal of Medicine 107, 1999, p. 588-94). This included a full 86 references, and went over seventeen different human studies using plant sterols to lower cholesterol since 1951 (Proceedings of the Society for Biological Medicine 78, 1951, p. 143-7). A total of 590 men and women were used in these studies with phytosterol therapy resulting in an average 10% reduction in total cholesterol and a 13% reduction in LDL cholesterol. They found this worked best in high-fat diets; the worse the diet the more results the researchers got. 4. Radix Rehmannia Glutinosa


• It is used to nourish the vital essence

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Research by scientists showed that Rehmannia improves blood movement. Effects of 50% ethanolic extract (JR-ext) from Chinese Rehmanniae Radix (the steamed and dried root of Rehmannia glutinosa, "Jyuku-Jio" in Japanese) on the hemorheology of inflammatory, thrombosic and intact animals were examined in the in vivo models. JR-ext (200 mg/kg, p.o.) inhibited the reduction of fibrinolytic activity and erythrocyte deformability, the decrease in erythrocyte counts and the increase in connective tissue of the thoracic artery in a chronic inflammatory model, adjuvant-induced arthritis. However, JR-ext was ineffective on the development of edema in the arthritic rats and on acute and chronic inflammation. JR-ext inhibited the reduction of erythrocyte deformability, but not the decrease of coagulative factors in a thrombosic model, endotoxin-induced disseminated intravascular coagulation (DIC). JR-ext also showed a promoting effect on erythrocyte deformability and fibrinolytic activity in intact rats. These results suggest that orally administered JR-ext can prevent an inducement of impediment in the peripheral microcirculation of various chronic diseases through the improvement of hemorheology (blood movement). “Studies on rehmanniae radix. I. Effect of 50%

ethanolic extract from steamed and dried rehmanniae radix on hemorheology in arthritic and

thrombosic rats.” Biol Pharm Bull (1994) 17: 1282-6.

5. Fructus Cornus Officinalis


• It is used to replenish the vital essence of the liver and kidneys a) In our previous study, we reported the renoprotective effect of Corni Fructus (Cornus officinalis SIEB. et ZUCC. In this study, we investigated the effects of morroniside isolated from Corni Fructus on renal damage in streptozotocin-treated diabetic rats. Oral administration of morroniside at a dose of 20 or 100 mg/kg body weight/d for 20 d to diabetic rats resulted in significant decreases in increasing serum glucose and urinary protein levels. Moreover, the decreased levels of serum albumin and total protein in diabetic rats were significantly increased by morroniside administration at a dose of 100 mg/kg body weight/d. In addition, morroniside significantly reduced the elevated serum urea nitrogen level and showed a tendency to reduce creatinine clearance. Morroniside also significantly reduced the enhanced levels of serum glycosylated protein, and serum and renal thiobarbituric acid-reactive substances. Protein expressions related to the advanced glycation endproduct (AGE) level and actions, oxidative stress such as N(epsilon)-(carboxyethyl)lysine, as well as receptors for AGE and heme oxygenase-1 were increased in diabetic rats, but the levels were also significantly decreased by the administration of morroniside. This suggests that morroniside exhibits protective effects against diabetic renal damage by inhibiting hyperglycemia and oxidative stress. These results indicate that morroniside is one component partly responsible for the protective effects of Corni Fructus and Hachimi-jio-gan against diabetic renal damage. “Protective Effects of Morroniside Isolated from

Corni Fructus against Renal Damage in Streptozotocin-Induced Diabetic Rats.” Biol Pharm

Bull. 2008 Jul;31(7):1422-8

b) Clinical results demonstrate that Fructus corni can promote a protective antioxidant defense



damage in the kidneys. The present study determined the effects of Fructus corni extract (FCE) on the levels of hydrogen peroxide (H2O2) and superoxide anion (O2-), on the glutathione (GSH) redox cycle and on the activities of antioxidant enzymes in bovine pulmonary artery endothelial cells (PAECs). 2. Confluent monolayers of PAECs were incubated with FCE, and oxidative stress was triggered by hypoxanthine and xanthine oxidase (to induce H2O2) or H2O2 (to induce O2-). 3. FCE exhibited a concentration-dependent suppression of H2O2 and O2-. 4. It modulated the GSH redox cycle by increasing the intracellular GSH content, the activities of GSH peroxidase and GSH disulfide reductase, and by decreasing the intracellular level of GSH disulfide. 5. It also increased the activities of superoxide dismutase and catalase. 6. These results demonstrate that FCE can promote a protective antioxidant defense state by affecting some important enzymatic and nonenzymatic oxidant-scavenging systems and may thus be useful for the prevention or treatment of disorders associated

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with oxidative damage. “Fructus corni enhances endothelial cell antioxidant defenses”. Gen

Pharmacol. 1998; 31(2):221-5

6. Semen Cuscutae

Function of the herb

! Invigorates the reproductive system ! To replenish liver and kidney ! To improve eyesight

a) Clinical research showed that Semen Cuscutae can be used to treat male sterility. To observe the effects of Semen Cuscutae (SC), Rhizoma Curculiginis (RC) and Radix Morindae Officinalis (RMO) on human sperm motility in vitro and cytomembrane function. METHODS: Compare the sperm motility of the above-mentioned medicinal plants with modified Tyrode's solution (MTS) and MTS added caffeine in vitro. Human spermatozoa were incubated with decoctions of medicinal plant at various concentrations in 37 degrees C for 30 minutes. The motility of sperm was evaluated by the sperm capillary penetrating test, sperm speed test and sperm activity index calculations. The spermatozoa membrane function was evaluated by spermatozoal hypo-osmotic swelling test together with eosin staining methods. RESULTS: The sperm motility improved markedly and sperm membrane function became more stabilized after incubation, the SC had best effect, RC took second place, RMO was relatively poor. CONCLUSIONS: SC decoction is an effective promoting preparation for sperm motility and the membrane function stabilized. Therefore, SC may be beneficial in treating male sterility and may raise success rate in artificial insemination. “Effects of semen

Cuscutae, rhizoma Curculiginis, radix Morindae officinalis on human spermatozoan's motility

and membrane function in vitro “.10035370 Zhongguo Zhong Xi Yi Jie He Za Zhi. 1997 Mar

;17 (3):145-7

b) To study the mechanism of flavonoids from Semen Cuscutae (FSC) improving the ovarian endocrine functions. METHODS: (1) FSC were obtained from the semen of Cuscuta chinensis through solvent extraction and polyamide columnar chromatography. (2) Sound, light and electricity were combined into a stressful stimulas to induce psychologic stress in female rats. (3) To observe the effects of FSC on the changes of beta-EP in hypothalamuses, FSH and LH in anterior pituitaries and the changes in pituitary morphology in female rats exposed to psychologic stress. RESULTS: FSC decreased the content of beta-EP in hypothalamuses, and increased the numbers of basophilic cell and content of LH in anterior pituitaries, didn't change content of FSH in anterior pituitaries in female rats exposed to psychologic stress. CONCLUSION: FSC decreases the contents of beta-EP in hypothalamuses and increases contents of LH in anterior pituitaries in female rats exposed to psychologic stress, which may be one of the mechanism of FSC improving hypothalamus-pituitary-ovary axis. “Effects of flavonoids from semen Cuscutae on changes of beta-EP in

hypothalamuses and FSH and LH in anterior pituitaries in female rats exposed to psychologic

stress.” Zhong Yao Cai. 2002 Dec ;25 (12):886-8 12685350 (P,S,E,B)

7. Herba Cistanche Deserticola


! To reinforce the vital function of the kidney especially that of the sexual organ

a) To investigate the pharmacological basis of the “Yang-invigorating” action of Herbal Cistanche [the dried whole plant of Cistanche deserticola Y.C. Ma (Orobanchaceae)], in Chinese medicine, the effects of the methanol extract of Herba Cistanche on mitochondrial ATP generation capacity were examined using an ex vivo rat heart model and an in situ H9c2 cell assay. Treatment with Herba Cistanche extract increased the myocardial mitochondrial ATP generation capacity in a dose-dependent manner in rats, as assessed by in vitro measurement. The stimulation of ATP generation capacity was associated with a parallel enhancement in mitochondrial electron transport supported by

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pyruvate but not succinate. Herba Cistanche treatment also increased the myocardial mitochondrial complex I and complex III activities, with the extent of stimulation on complex I activity being larger. Herba Cistanche treatment produced a dose-and time-dependent increase in mitochondrial ATP generation capacity in H9c2 cells. The results indicate that Herba Cistanche treatment can increase mitochondrial ATP generation in rat hearts ex vivo and H9c2 cells in situ, possibly through enhancing the oxidative phosphorylation. “Herba Cistanche Extract Enhances Mitochondrial ATP

Generation in Rat Hearts and H9c2 Cells” Pharmaceutical Biology, Volume 46, Issue 6 June

2008 , pages 418 - 424

b) Chinese tonifying herbs such as Herba Cistanche, which possess antioxidant and/or immunomodulatory activities, can be useful in the prevention and treatment of age-related diseases. Pharmacological studies on Yang and Yin tonifying herbs suggest that Yang tonifying herbs stimulate mitochondrial adenosine triphosphate (ATP) generation, presumably through the intermediacy of reactive oxidant species, leading to the enhancement of cellular/mitochondrial antioxidant status. Yin tonifying herbs, however, apart from possessing antioxidant properties, exert mainly immunomodulatory functions that may boost a weak immune system and may also suppress overreactive immune responses. The abilities of Yang and Yin Chinese tonifying herbs to enhance ATP generation and to exhibit antioxidant and/or immunomodulatory actions are the pharmacological basis for their beneficial effects on the retardation of aging. “Enhancement of ATP generation

capacity, antioxidant activity and immunomodulatory activities by Chinese Yang and Yin

tonifying herbs.” Chin Med. 2007; 2: 3. doi: 10.1186/1749-8546-2-3.

8. Flos Carthami


! It is used to stimulate blood flow a) The apoptotic death of cardiomyocytes due to ischemia/reperfusion is one of the major complications of heart disease. Ischemia/reperfusion has been shown to lead to the activation of the stress-activated protein (SAP) kinases and the p38/reactivating kinase (p38/RK). In this study, the direct effect of an aqueous Flos carthami (FC) extract on SAP kinases was investigated. When isolated rat hearts were perfused by Langendorff mode with media containing FC extract prior to the induction of global ischemia and the subsequent reperfusion, SAP kinase activity was inhibited 95%. Untreated ischemic/reperfused hearts showed a 57% elevation in the activity of SAP kinase. The in

vitro effect of these FC extracts on SAP kinase was also tested. At a concentration of 10 g/ml, the aqueous FC extract resulted in 50% inhibition of SAP kinase activity in ischemic heart tissue. Our results showed that FC affected both the interaction of SAP kinase with c-jun as well as the phosphotransferase reaction. These results clearly demonstrate that extracts from Flos carthami exerted inhibitory effects on SAP kinase. The administration of the FC extract may lead to a modulation of the apoptotic effect of SAP kinase activation induced during ischemia/reperfusion.

“Effect of Flos carthami on stress-activated protein kinase activity in the isolated reperfused rat

heart.” Molecular and Cellular Biochemistry Volume 207, Numbers 1-2 / April, 2000

10.1023/A:1017266628572

9. Herba Epimedii


! To reinforce the vital function of the kidney especially that of the sexual organ

a) Objective: study the influencing rules about ()~(45)Ca influx and efflux of rat's principal visceral organs with the different extracts; study the effect of Herba Epimedil's extracts on myocardial ischemia injury in rats. Method: 1.wide aperture resin were used to isolate chemical components; 2. ()~(45)Ca transmembrane influx technique was used to study influencing rules and substance

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foundation. Results: 1.the extracts of Herba! Epimedii have significant effects on PDC ()~(45)Ca channel of three visceral organs in rats: heart, artery, smooth muscle and kidney. They not only block ()~(45)Ca coming into cells, but also can facilitate efflux of ()~(45)Ca in the cell. 2. the regulating effect about the extracts by alkali is better than those by alcohol and water. 3. Herba Epimedii extracts possess significant protection effects on myocardial ischemia induced either by drugs or by ligating the coronary artery. Conclusion: these results suggested that Herba Epimedii had significant regulating effect on PDC ()~(45)Ca channel, that Herba Epimedii possesses significant protection effects on myocardial ischemia. “Effect of Herba Epimedii on the Visceral Organs and

Myocardial Ischemia Injury in Rats.” Journal of Sichuan University (Natural Science Edition)

February 28, 2005

b) Herba epimedii (HEP) is one of the most frequently used herbs prescribed for treatment of osteoporosis in China. In the present study, the in vivo effects of HEP extract on bone metabolism

were evaluated using 4-month-old ovariectomized (OVX) or sham-operated (Sham) female Sprague-Dawley rats orally administered with HEP extract (110 mg kg–1d–1), 17ß-estrogen (2 mg kg–1d–1) or its vehicle for 3 months. HEP extract significantly decreased urinary calcium excretion, suppressed serum alkaline phosphatase (ALP) activity and urinary deoxypyridinoline levels in OVX rats (P < 0.05 versus vehicle-treated OVX rats). Histomorphometric analysis indicated that HEP extract could

prevent OVX-induced bone loss by increasing tibial trabecular bone area and decreasing trabecular separation in OVX rats (P < 0.05 versus vehicle-treated OVX group). The in vitro effects of HEP extract were also studied using rat osteoblast-like UMR 106 cells. HEP extract significantly stimulated cell proliferation in a dose-dependent manner (P < 0.01 versus vehicle-treated) and increased ALP activity at 200 $gml–1 (P < 0.01 versus vehicle-treated) in UMR 106 cells. It modulated

osteoclastogenesis by increasing osteoprotegrin (OPG) mRNA and decreasing receptor activator of NF- B ligand (RANKL) mRNA expression, resulting in a dose-dependent increase in OPG/RANKL mRNA ratio (P < 0.01 versus vehicle-treated). Taken together, HEP treatment can effectively suppress the OVX-induced increase in bone turnover possibly by both an increase in osteoblastic activities and

a decrease in osteoclastogenesis. The present study provides the evidence that HEP can be considered as a complementary and alternative medicine for treatment of post-menopausal osteoporosis. “The

osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro.” Oxford Journal

Evidenced-based Complimentary and Alternative Medicine CAM 2005 2(3):353-361;

doi:10.1093/ecam/neh101

c) The effects of tonifying the kidney and strengthening the "Yang" produced by the extracts of Chinese herbs Epimedium wushanense and E. pubescens were studied in this paper. The results showed that both two herbs could decrease the concentration of plasma middle molecular substances and increase the concentration of plasma sulfhydryl group of "Yang-deficiency" model mice, thus suggesting that the above-said tonifying and strengthening effects of Herba Epimedii may result from its effects on middle molecular substances and sulfhydryl group, so as to strengthen the body resistance and eliminate the invading pathogenic factors. “Effects of Sichuan herba Epimedii on the

concentration of plasma middle molecular substances and sulfhydryl group of "yang-

deficiency" model animal.” Zhongguo Zhong Yao Za Zhi 1995 Apr;20(4):238-9, 254 PMID:

7646796

d) The active constituents of Herba Epimedii can raise the hypothalamic monoamine neurotransmitters levels in aging male rats and improve the learning and memory in aging rats and mice. It can also inhibit the activities of AchE both in brain tissues and whole blood in mice. Effect of

active constituents of Herba Epimedii on hypothalamic monoamine neurotransmitters and

other brain functions in aging ratsZhongguo Zhong Yao Za Zhi 1996 Nov;21(11):683-5 PMID:

9812700

e) In order to confirm the theory of administration at optimal time in The Yellow Emperor's internal

Classic, Dr. Wang Fei, Dr. Zheng Yang and Xiao Honbin, et al (Helongjiang University of Traditional Chinese Medicine, Harbin, China ) have investigated effects of administration of Herba Empimedii at the optimal time on the level of sexual hormones in young mice to explore the

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relationship between the time of administration and the effect. Results indicated that (1) Herba Epimedii decoction had sexual hormone like actions, increasing weight of uterus and serum estradiol ( E2 ) content in the female mice, increasing serum testosterone ( T ) content in the male mice ; (2) The administration at Mao ( the period of the day from 5am to 7am ) and You ( from 5pm to 7pm ) hours influenced the increases of uterus weight and serum E2 content in the female mice and serum T content in the male mice to a certain degree, the action at Mao being stronger than that at the You. It is suggested that administration for tonifying the kidney and strengthening Yang in the early

morning has the best effect. Effect of Administration of Herba Epimedii on Levels of Sexual

Hormones. JOURNAL OF TRADITIONAL CHINESE MEDICINE Vol. 42 No. 10 October

2001)

f) Objective: To investigate the prevention effect of aqueous extract of Epimedii sagittatum (ESE) on ovariectomy-induced (OVX) bone loss in rats. Methods: Rats were divided into sham-operated and OVX groups. The OVX rats were divided into four groups treated with distilled water, 17#-estradiol (1 mg/kg, ig) and ESE (0.5 and 1 g /kg, ig) for 11 weeks. Serum calcium, phosphorus, estradiol, bone gla protein concentrations and serum alkaline phosphatase activity were measured. Bone density was assayed by dual-energy X-ray absorptiometry. The undecalcified longitudinal proximal tibial metaphysical sections were cut and stained for the bone histomorphometric analysis. Results: In OVX rats, alkaline phosphatase activity in serum was markedly increased by ESE treatment, which had no obvious influence on the body weight. Meanwhile, atrophy of uterus and descent of bone mineral density were suppressed by ESE treatment. In addition, ESE completely corrected the decreased concentrations of calcium and E2 in serum observed in OVX rats. Histological results also showed ESE prevented the increases in trabecular separation (Tb.Sp) in OVX rats whereas it did not alter trabecular thickness (Tb.Th) and trabecular number (Tb.N) in OVX rats. Moreover, ESE had remarkable effect on bone formation rate with bone volume as referent (BFR/BV) and bone formation rate with bone surface as referent (BFR/BS). Conclusion: The findings assessed on the basis of biochemical test, bone mineral density and histomorphometric parameters show that aqueous extract of Epimedii sagittatum has a definite antiosteoporotic effect and can prevent the OVX-induced bone loss in rats. “Prevention of bone loss

by aqueous extract of Epimedii sagittatum in an ovariectomized rat model of osteoporosis.”

Journal of Chinese Integrative Medicine: 2006; 4(6): 628-633 DOI: 10.3736/jcim20060618

g) Aim: To evaluate the testosterone mimetic properties of icariin. Methods: Forty-eight healthy male Sprague-Dawley rats at the age of 15 months were randomly divided into four groups with 12 rats each: the control group (C), the model group (M), the icariin group (ICA) and the testosterone group (T). The reproductive system was damaged by cyclophosphamide (intraperitoneal injection, 20 mg/kg!day) for 5 consecutive days for groups M, ICA and T, at the sixth day, ICA (gastric gavage, 200 mg/kg!day) for the ICA group and sterandryl (subcutaneous injection, 5 mg/rat!day) for the T group for 7 consecutive days, respectively. The levels of serum testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), serum bone Gla-protein (BGP) and tartrate-resistant acid phosphatase activity in serum (StrACP) were determined. The histological changes of the testis and the penis were observed by microscope with hematoxylin-eosin (HE) staining and terminal deoxynucleotidyl transferase biotin-dUTP-X nick end labeling (TUNEL), respectively. Results: (1) Icariin improved the condition of reproductive organs and increased the circulating levels of testosterone. (2) Icariin treatment also improved the steady-state serum BGP and might have promoted bone formation. At the same time, it decreased the serum levels of StrACP and might have reduced the bone resorption. (3) Icarrin suppressed the extent of apoptosis of penile cavernosal smooth muscle cells. Conclusion: Icariin has testosterone mimetic properties and has therapeutic potential in the management of hypoandrogenism. “The testosterone mimetic properties of icariin

from Herba Epimedii.” Asian Journal of Andrology Volume 8 Issue 5, Pages 601-605

h) AIM: To clarify the mechanism of the therapeutic action of icariin on erectile dysfunction (ED). METHODS: PDE5 was isolated from the human platelet and PDE4 from the rat liver tissue using the FPLC system (Pharmacia, Milton Keynes, UK) and the Mono Q column. The inhibitory effects of

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icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [(3)H]-cGMP/[(3)H]-cAMP. Papaverine served as the control drug. RESULTS: Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities. The IC(50) of Icariin and papaverine on PDE5 were 0.432 micromol/L and 0.680 micromol/L, respectively and those on PDE4, 73.50 micromol/L and 3.07 micromol/L, respectively. The potencies of selectivity of icariin and papaverine on PDE5 (PDE4/PDE5 of IC(50)) were 167.67 times and 4.54 times, respectively. CONCLUSION: Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED. “Effects of icariin from Herba Epimedii on cGMP-

specific PDE5 and cAMP-specific PDE4 activities.” Asian J Androl. 2003 Mar;5(1):15-8. PMID:

12646997

i) To further investigate the mechanisms of action of icariin (ICA), we assessed the effects of ICA on the in vitro formation of cGMP and cAMP in isolated rabbit corpus cavernosum. Isolated segments of rabbit corpus cavernosum were exposed to increasing concentrations of ICA and the dose-dependent accumulation of cGMP and cAMP was determined in the tissues samples by means of 125I radioimmunoassay. Responses of the isolated tissues preparations to ICA were compared with those obtained with the reference compounds sildenafil (Sild). Furthermore, the effects of ICA on the mRNA expression of specific cGMP-binding phosphodiesterase type V (PDE5) in rat penis were also observed. After incubation with ICA for 6 h or 14 h respectively, the levels of PDE5 mRNA were examined by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that ICA increased cGMP concentrations directly (P<0.05), but there was no significant effect on cAMP concentrations (P<0.05). In the presence of sodium nitroprusside (SNP), a stimulatory agent of cGMP, both ICA and Sild increased cGMP concentrations with increasing dose (P<0.01). Their EC50 was 4.62 (ICA) and 0.42 (Sild) µ mol/L respectively. Under the same condition, ICA and Sild unaltered cAMP level significantly (P>0.05). There were PDE5A1 and PDE5A2 mRNA expressions in rat corpus cavernosum with PDE5A2 being the dominant isoform. ICA could obviously inhibit these two isoforms mRNA expression in rat penis, and decrease PDE5A1 more pronouncedly (P<0.01). The present study indicated that the aphrodisiac mechanisms of icariin involved the NO-cGMP signal transduction pathway, with increasing cGMP levels in the corpus cavernosum smooth muscle. The inhibitory effect of icariin on PDE5 mRNA expression, especially on PDE5A1, might account for its molecular mechanisms for its long-term activity. “Effect of icariin from Herba

Epimedii on cyclic GMP levels and on the mRNA expression of cGMP-binding cGMP-specific

phosphodiesterase (PDE5) in penile cavernosum.” Journal of Huazhong University of Science

and Technology Volume 26, Number 4 / September, 2006 10.1007/s11596-006-0421-y

j) The study the effects of oral administered icariin on intracavernosal pressure (ICP) and on expression of the nitrogen oxide synthase (NOS) isoforms in corpus cavernosum (CC) of arteriogenic erectile dysfunction (A-ED) rat model. METHODS: Forty adult male Wistar rats were randomly divided into 4 groups of 10 rats: shame operated group (group A) and three A-ED model groups (group B, C and D). The internal pudendal arteries were isolated and ligated with 7-O nylon thread at both the main trunk and the penile branches to establish the A-ED model. ICP were tested after the operation to make sure the successful model establishment. The groups A and B were treated with saline: and the groups C and D were treated with icariin (5 mg/kg/day and 10mg/kg/day respectively) orally for 30 days. Then the ICP was measured again. The tissues of corpus cavernosum were taken and RT-PCR was used to detect the mRNA expression of nNOS, iNOS and eNOS in CC, and Western-blot was used to detect the protein expression of these NOS isoforms. RESULTS: The ICP in the group B was significantly decreased compared to the group A (P < 0.01), but the ICP values in the groups C and D were both increased compared to those in the group B (both P < 0.01). The expressions of the mRNA and protein of nNOS, iNOS, and eNOS were all decreased in the group B, however, the mRNA and protein expressions of eNOS were increased a in the groups C and D. In the group C, iNOS also increased. The expression of nNOS showed no obvious changes in the group C and group D. CONCLUSION: Chronic oral treatment with Icariin increases the erectile function (ICP) and restores the eNOS expression in CC of A-ED rats. Icariin may have a long-term therapeutic effect on ischemia/hypoxia induced ED. “Effects of icariin from Herba Epidemiic on the erectile

function and expression of nitrogen oxide synthase isoforms in corpus cavernosum of

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arterigenic erectile dysfunction rat model.” Zhongguo Zhong Yao Za Zhi 2004 Jun

2;84(11):954-7. PMID: 15329286

10. Rhizoma Ligusticum Chuanxiong


• It is used to invigorate blood circulation and promote the flow of vital energy a) Ligustilide is the most abundant bioactive ingredient in Rhizoma Chuanxiong, a Chinese medicinal herb commonly used for the treatment of cardiovascular ailments. The present study reported, for the first time, the pharmacokinetics of ligustilide, administered in its pure form and in an herbal extract, in rats. After i.v. administration of pure ligustilide, it was distributed extensively (Vd, 3.76 ± 1.23 l/kg) and eliminated rapidly (t1/2,





10.1124/dmd.107.017707


in blood vessels, a key factor for erection to take place: this is how Viagra works) via inhibiting

voltage dependent calcium channel and receptor-mediated Ca2+

influx and release.” Vascular

Pharmacology ISSN 1537-1891 2006, vol. 45, n 3, pp.171-176

c) Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5"105 µl. HAAKE rheometer RS 600

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with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6±4.6% of the control to 12.5±2.1% in the presence of TMP (1.46 mM) and SAB (10 µM) (P<0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress. “Inhibition of shear-induced platelet aggregation in rat by tetramethylpyrazine and salvianolic acid B, active component of Rhizoma Ligustici Chuanxiong”. Clinical Hemorheology and Microcirculation Vol. 31, Number 2/2004 Pages 97-103 11. Radix Dipsacus Asper


• It is used to replenish the liver and kidneys The methanol extract from Dipsacus asper Wall (Dipsacaceae) was found to show antioxidant activity against free radical and Cu(2+)-mediated LDL oxidation. In further study, to identify active constituents from the plant, six caffeoyl quinic acid derivatives: 3,4-di-O-caffeoylquinic acid (1), methyl 3,4-di-O-caffeoyl quinate (2), 3,5-di-O-caffeoylquinic acid (3), methyl 3,5-di-O-caffeoyl quinate (4), 4,5-di-O-caffeoylquinic acid (5) and methyl 4,5-di-O-caffeoyl quinate (6) were isolated. Their structures were identified by spectroscopic methods including 2D-NMR. The isolated compounds, 1-6, were found to be potent scavengers of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), and are more potent than butylated hydroxyl toluene (BHT) used as a positive control. The compounds 1-6 also inhibited Cu(2+)-mediated low-density lipoprotein (LDL) oxidation. They increased the lag time of conjugated dienes formation and inhibited the generation of thiobarbituric acid reactive substances (TBARS) in a dose-dependent manner. These results suggested that Dipsacus asper due to its antioxidant constituents, 1-6, may have a role to play in preventing the development and progression of atherosclerotic disease. “The antioxidant activity of caffeoyl quinic acid from

the roots of Dipsacus asper.” J Ethnopharmacol. 2006 May 22; : 16809011



• It is used to nourish the blood a) Paeonia lactiflora and Astragalus membranaceus are two popular traditional Chinese medicines, commonly used in Chinese herb prescription to treat liver disease. The extract prepared from the roots of Paeonia lactiflora and Astragalus membranaceus (PAE) demonstrated more excellent hepato-protective activity than the single herbs used individually as indicated in our preliminary studies. The present study was carried out to investigate the effects of PAE on liver fibrosis in rats induced by carbon tetrachloride (CCl4) and to explore its possible mechanisms. Liver fibrosis was induced in male Sprague–Dawley rats by injection with 50% CCl4 subcutaneously twice a week for 8 weeks. At the same time, PAE (40, 80 and 160 mg/kg) was administered intragastrically. Upon pathological examination, the PAE-treated rats significantly reduced the liver damage and the symptoms of liver fibrosis. Administration of PAE decreased CCl4-induced elevation of serum transaminase activities, hyaluronic acid, laminin and procollagen type III levels, and contents of hydroxyproline in liver tissue by approximately 30–60%. It also restored the decrease in SOD and GSH-Px activites and inhibited the formation of lipid peroxidative products during CCl4 treatment. Moreover, PAE (80, 160 mg/kg, ig) decreased the elevation of TGF-#1 by 47.7% and 53.1%, respectively. In the primary cultured hepatic stellate cells (HSCs), PAE also significantly decreased [3H] thymidine incorporation in cells stimulated with platelet-derived growth factor-B subunit homodimer (PDGF-BB) and suppressed [3H] proline incorporation. These results suggested that PAE significantly inhibited the progression of hepatic fibrosis induced by CCl4, and the inhibitory effect of PAE on hepatic fibrosis might be associated with its ability to scavenge free radicals, decrease the level of TGF-#1 and inhibit collagen synthesis and proliferation in HSCs. “Effects and mechanisms of extract from Paeonia lactiflora

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and Astragalus membranaceus on liver fibrosis induced by carbon tetrachloride in rat.”

Journal of Enthnopharmacology Volume 112, Issue 3 25 July 2007, Pages 514-523

b) This study examined the effect of Paeoniae Radix (PR) on endothelial function and the activity of superoxide dismutase (SOD) of erythrocytes in rats administered a high-fat diet. Administration of the extract of PR increased the endothelium-dependent relaxation and the activities of SOD compared with high the cholesterol diet group significantly. Hypercholesterolaemia induced an increase of endothelial superoxide anion and endothelial dysfunction. Paeoniae Radix is suggested to have a protective effect on endothelial cells and their function. “Effect of extract prepared from the roots

of Paeonia lactiflora on endothelium-dependent relaxation and antioxidant enzyme activity in

rats administered high-fat diet.” Phytother Res. 1999 Sep;13(6):526-8

(The endothelium is the thin layer of cells that line the interior surface of blood vessels. Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. These cells reduce turbulence of the flow of blood allowing the fluid to be pumped farther.) c) Paeoniae Radix is a crude drug that is used in Asia and Europe to improve blood flow. We studied its vasodilator effect and mechanisms of action in vitro. The extract from Paeoniae Radix (PRE) relaxed prostaglandin F2a-precontracted aortic ring preparations of isolated rat aorta that contained endothelium. Relaxation by PRE did not occur in specimens without endothelium, and was inhibited by pretreatment with 10-4 M NG-nitro-1-arginine methyl ester. Paeoniflorin and paeonol, the main active components of Paeoniae Radix, lacked a vasodilator effect. The effect of the component gallotannin was examined after treating PRE with tannase, but the product lacked a vasodilator effect. Pentagalloylglucose, hexagalloylglucose, heptagalloylglucose, and octagalloylglucose were extracted from PRE ; they relaxed aortic rings with endothelium, but failed to relax aortic rings without endothelium. We conclude that PRE exhibits an endothelium-dependent vasodilator effect on isolated rat aorta. “Endothelium-dependent vasodilator effect of extract prepared from the roots of

Paeonia lactiflora on isolated rat aorta.” Planta medica ISSN 0032-0943 1996, vol. 62, n 5, pp.

436-439 (21 ref.)

d) Research showed that the herb: 1) Lower cholesterol levels. “Zhang Y. The effects of nifedipine, diltiazem, and Paeonia lactiflora Pall. on atherogenesis in rabbits. Chung Hua Hsin Hsueh Kuan Ping Tsa Chih 1991;19:100–3” 2) Improve liver function and help prevent liver damage from chemical toxins such as carbon tetrachloride. “Qi XG. Protective mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage. Chung Hsi I Chieh Ho Tsa Chih 1991;11:69, 102–4” 3) Inhibit blood platelet aggregation. “Liu J. Effect of Paeonia obovata 801 on metabolism of thromboxane B2 and arachidonic acid and on platelet aggregation in patients with coronary heart disease and cerebral thrombosis. Chung Hua I Hsueh Tsa Chih (Chin Med J) 1983;63:477–81.” e) Clinical research by western scientists confirmed the validity of the historical use of the drug,

Radix Paeonia Lactiflora for improving liver functions by preventing liver damage and micro-

circulation disorders. "Acute liver damage from D-galactosamine was initiated in rats that were then treated with Paeonia lactiflora and Salvia miltiorrhiza. Parameters measured in this study were changes in ALT, bilirubin, levels of plasma fibronectin and pathological histology. The results showed that both herbs increased plasma fibronectin and improved the reticuloendothelial system function. Aggregation of albumin, collagen fragments and immune complexes were markedly reduced. Liver immune damage and micro-circulation disorders were avoided, justifying the important role of Paeonia lactiflora and Salvia miltiorrhiza in protecting hepatocytes". Protective

mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage. Zhong

Xi Yi Jie He Za Zhi 1991 Feb;11(2):102-4, 69. PMID: 2060049



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! Promote blood circulation ! Treat coronary heart disease ! Increase red and white blood cells ! Protect liver ! Improve function of liver and spleen

a) Research by western scientists confirmed that Salvia Miltiorrhiza extracts are effective in

preventing the development of bone loss. The preventive effect of Salvia miltiorrhiza extracts (SMEs) on the progress of bone loss induced by ovariectomy (OVX) was studied in rats. We measured body weight and bone histomorphometry in sham, OVX or SMEs-administered OVX rats. From light microscopic analyses, a porous or erosive appearances were observed on the surface of trabecular bone of tibia in OVX rats, whereas those of the same bone in sham rats and in SMEs-administered rats were composed of fine particles. The trabecular bone area and trabecular thickness in OVX rats decreased by 50% from those in sham rats, these decreases were completely inhibited by administration of SMEs for 7 weeks. In this study, the mechanical strength in femur neck was significantly enhanced by the treatment of SMEs for 7 weeks. In OVX rats, free T3 was normal in all cases, whereas free T4 was significantly increased. Although there was no difference between OVX and SMEs-administered rats in T3 level, we have found significant difference between them in T4 level. These results strongly suggest that SMEs are effective in preventing the development of bone loss induced by OVX in rats. “Prevention of bone loss in ovariectomized rats: the effect of Salvia

miltiorrhiza extracts.” Immunopharmacol Immunotoxicol 2004 Feb;26(1):135-44. Department

of Pharmacology, Institute of Cardiovascular Research, School of Medicine, Chonbuk National

University, South Korea. (PMID: 15106738)

b) Clinical research by western scientists from Taiwan showed that Salvia Miltiorrhiza exerts

antifibrotic effects and confirmed the validity of the historical use of the herb for improving

liver functions. Stronger liver would reduce kidney stress. Excessive oxidative stress is implicated in hepatic fibrogenesis. Extracts of Salvia miltiorrhiza (Sm) have been shown to protect cells against oxidative stress. In this study we investigated the in vitro and in vivo effects of Sm on hepatic fibrosis. A cell line of rat hepatic stellate cells (HSC-T6) was stimulated with transforming growth factor-beta1 (TGF-beta1). The inhibitory effects of Sm (50-400 microg/ml) on TGF-beta1-induced alpha-smooth muscle actin (alpha-SMA) secretion and the mRNA expressions of fibrosis-related genes, including alpha-SMA, connective tissue growth factor (CTGF), and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed. Fibrosis was induced by dimethylnitrosamine (DMN) administration in rats. DMN-treated rats were randomly assigned to 1 of 4 groups: saline, Sm (20 mg/kg), Sm (100 mg/kg), or silymarin (100 mg/kg), each given by gavage twice daily for 5 weeks starting from the onset of DMN administration. Sm (200 and 400 microg/ml) significantly inhibited TGF-beta1-stimulated alpha-SMA secretion and the mRNA expressions of alpha-SMA, CTGF, and TIMP-1 in HSC-T6 cells. Fibrosis scores of livers from DMN-treated rats with either a low (1.8 +/- 0.2) or high (1.8 +/- 0.1) dose of Sm, or silymarin (1.4 +/- 0.2) were significantly reduced in comparison with DMN-treated rats receiving saline (3.1 +/- 0.1). Hepatic collagen contents were also significantly reduced by either Sm or silymarin treatment. The mRNA expression levels of alpha-SMA, TGF-beta1, and procollagen I were all attenuated in Sm- and silymarin-treated rats. Moreover, levels of plasma aspartate transaminase activities were reduced by Sm and silymarin treatment. In conclusion, our results show that Sm exerted antifibrotic effects in both HSC-T6 cells and in rats with DMN-induced fibrosis. “Antifibrotic effects of Salvia miltiorrhiza on dimethylnitrosamine-intoxicated rats.” J.

Biomed Sci 2005;12(1):185-95 Institute of Traditional Medicine, School of Medicine, National

Yang-Ming University, Taipei, Taiwan PMID: 15864749

c) Clinical research by western scientists confirmed the validity of the historical use of the drug,

Salvia Miltiorrhiza for improving liver functions by preventing liver damage and micro-

circulation disorders. “Acute liver damage from D-galactosamine was initiated in rats that were then treated with Paeonia lactiflora and Salvia miltiorrhiza. Parameters measured in this study were changes in ALT, bilirubin, levels of plasma fibronectin and pathological histology. The results

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showed that both herbs increased plasma fibronectin and improved the reticuloendothelial system function. Aggregation of albumin, collagen fragments and immune complexes were markedly reduced. Liver immune damage and micro-circulation disorders were avoided, justifying the important role of Paeonia lactiflora and Salvia miltiorrhiza in protecting hepatocytes.” "Protective

mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage", Chung

His Ho Tsa Chih, 11(2), pp 102-4, 1991.

d) Clinical research by western scientists confirmed the validity of the historical use of the drug

for improving liver functions. “Salvia miltiorrhiza (Sm) administration has been shown to reduce hepatic fibrosis in rats. We investigated the hemodynamic effects of Sm on bile duct ligated (BDL) rats. Hemodynamic, histological, and vascular contractile studies were conducted in rats 4 weeks after bile duct ligation. An aqueous extract of Sm (0.2 g twice per day) or vehicle was administered for 4 weeks to BDL rats. Sm treatment in BDL rats significantly reduced histological grades of fibrosis and ameliorated the portal hypertensive state (including portal venous pressure, superior mesenteric artery blood flow, cardiac index, and total peripheral resistance) as compared with vehicle treatment. Moreover, Sm treatment enhanced the vascular sensitivity of mesenteric arteries to phenylephrine in BDL rats. Sm treatment had no effect on plasma biochemical profiles of either BDL or normal rats. Our results suggest that 4-week Sm treatment ameliorates the portal hypertensive state in BDL rats.Key words: hemodynamics, fibrosis, portal hypertension, vascular contractile response, Salvia miltiorrhiza.” “Hemodynamic effects of Salvia miltiorrhiza on cirrhotic rats.” Canadian Journal

of Physiology and Pharmacology, Volume 79, Number 7, July 2001 , pp. 566-572(7)

e) Clinical research by western scientists have shown that Salvia miltiorrhiza can be used to

treat stroke patients and heart diseases. “According to the theory of traditional Chinese medicine, cerebral infarction results from blood stasis, and the method of quickening the blood and dispelling stasis is used to treat cerebral infarct. salvia miltorrhiza bunge (SM) is a Chinese herb, which is considered to have an action of quickening the blood and dispelling stasis, and is frequently used to treat related disorders of blood stasis such as cerebrovascular accident and ischemic heart disease. The aim of the present study was to investigate the effect of SM on cerebral infarct in ischemia-reperfusion injured rats. A total of 30 Sprague-Dawley (SD) rats were studied. A model of focal cerebral infarct was developed by occluding both common carotid arteries and the right middle cerebral artery for 90 minutes. After 24 hours reperfusion, the rats were killed and the brain tissue was stained with 2, 3, 5-triphenyl-tetrazolium chloride (TTC). The areas of cerebral infarct were calculated, and lumino-chemiluminesence (CL) counts and lucigenin-CL counts of peripheral blood taken at this time were measured. The changes in the area of cerebral infarct were used as an index to evaluate the effect of SM on cerebral infarct. The results indicated that pretreatment with intraperitoneal injection of 30 mg/kg and 15 mg/kg SM reduced the area of cerebral infarct and also reduced the luminol-CL counts of peripheral blood in ischemia-reperfusion injured rats. This study has demonstrated that SM can reduce the area of cerebral infarct in ischemia-reperfusion injured rats, suggesting it may be useful in the treatment of cerebral infarct in humans. The therapeutic effect of SM may be partly due to its free radical scavenging activities.” “Effect of salvia miltiorrhiza bunge

on cerebral infarct in ischemia-reperfusion injured rats.” Am J Chin Med. 2003;31(2):191-200

Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan, ROC.. PMID:

12856858

f) Clinical research showed that Salvia miltiorrhiza induced apoptosis in HL60 human

premyelocytic leukemia cell line. Apoptosis is a new therapeutic target of cancer research. Tanshinone IIA isolated from Salvia miltiorrhiza BUNGE, a traditional oriental medical herb, was observed to induce apoptosis in HL60 human premyelocytic leukemia cell line. Tanshinone IIA induced DNA fragmentation into the multiples of 180 bp and increased the percentage of hypodiploid cells in flow cytometry after propidium iodide (PI) staining. Tanshinone IIA-induced apoptosis is accompanied by the specific proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) and the activation of caspase-3, a major component in apoptotic cell death mechanism. “Tanshinone IIA

isolated from Salvia miltiorrhiza BUNGE induced apoptosis in HL60 human premyelocytic

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leukemia cell line.” J. Ethnopharmacol 1999 Dec 15;68(1-3):121-7. Laboratory of Cancer

Research, Korea Institute of Oriental Medicine, Seoul, South Korea. PMID: 10624871

g) Clinical research by western scientists confirmed Salvia Miltiorrhiza has antioxidative agent

by inhibiting the production of nitric oxide (NO), interleukin-1beta (IL-1beta), interleukin-6

(IL-6) and tumor necrosis factor-alpha (TNF-alpha), and suppressed the expression of inducible

nitric oxide synthase (iNOS). “The inhibitory effects of tanshinone IIA, a diterpene isolated from Salvia miltiorrhiza root, on the production of nitric oxide (NO), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), and the expression of inducible nitric oxide synthase (iNOS) were investigated in activated RAW 264.7 cells. This compound markedly inhibited the production of NO, IL-1beta and TNF-alpha, and suppressed the expression of iNOS in a dose-dependent manner. These results suggest that the traditional use of S. miltiorrhiza as an anti-inflammatory herbal medicine may be explained, in part, by the inhibition of NO, IL-1beta, IL-6 and TNF-alpha production, and expression of iNOS.” “Tanshinone IIA from Salvia

miltiorrhiza inhibits inducible nitric oxide synthase expression and production of TNF-alpha,

IL-1beta and IL-6 in activated RAW 264.7 cells.” Planta Med. 2003 Nov;69(11):1057-9

Department of Oral Microbiology, School of Dentistry, Wonkwang University, Iksan, Chonbuk,

South Korea. PMID: 14735448

h) Clinical research by western scientists have shown that Tanshinone I from Salvia

miltiorrhiza has in vivo anti-inflammatory activity. The methanol extract of Salvia miltiorrhiza

was found to inhibit PGD2 production and Tanshinone I was found to inhibit PGE2 formation.

“Arachidonic acid (AA) mainly released from the cell membrane by phospholipase A2 (PLA2) is converted to eicosanoids by the action of cyclooxygenase (COX) and lipoxygenase (LO). In order to find the specific inhibitors of AA metabolism especially PLA2 and COX-2, 300 plant extracts were evaluated for their inhibitory activity on PGD2 production from cytokine-induced mouse bone marrow-derived mast cells in vitro. From this screening procedure, the methanol extract of Salvia

miltiorrhiza was found to inhibit PGD2 production and the ethyl acetate subfraction gave the strongest inhibition of five subfractions tested. From this ethyl acetate subfraction, an activity-guided isolation finally gave tanshinone I as an active principle. This investigation deals with the effects of tanshinone I on AA metabolism from lipopolysaccharide (LPS)-induced RAW 264.7 cells and in vivo antiinflammatory activity. Tanshinone I inhibited PGE2 formation from LPS-induced RAW macrophages (IC50 = 38 M). However, this compound did not affect COX-2 activity or COX-2 expression. Tanshinone I was found to be an inhibitor of type IIA human recombinant sPLA2(IC50 = 11 M) and rabbit recombinant cPLA2 (IC50 = 82 M). In addition, tanshinone I showed in vivo antiinflammatory activity in rat carrageenan-induced paw oedema and adjuvant-induced arthritis.” “Effects of tanshinone I isolated from Salvia miltiorrhiza Bunge on arachidonic

acid metabolism and in vivo inflammatory responses” Korea Phytotherapy Research. Volume

16, Issue 7, Pages 616-620 Natural Products Research Institute, Seoul National University, Seoul

110-460,

i) Clinical research by western scientists have shown that hydroxyl radical seems to be the most

important factor in ischemia (reduce blood supply) and reperfusion (damage to tissue caused

when blood supply returns to the tissue after a period of ischemia) injury to skeletal muscle and

that Salvia Miltiorrhiza have a role in antioxidation as a hydroxyl radical scavenger. “The extract of salvia miltiorrhiza radix (SMR) is a well known Chinese herbal medicine known as danshen. It has been found in several experimental studies, published mostly in China, to have antioxidant properties. The purpose of this experiment was, therefore, to compare the effects of danshen injection with those of superoxide dismutase (SOD) and mannitol on skeletal muscle ischemia and reperfusion injury in rabbits. The authors used a rabbit limb replantation model subjected to 5 h of global ischemia. Immediately before reperfusion, the animals received SOD (16,000 U/kg), mannitol (1 g/kg) or danshen injection (2 g/kg) intravenously. Both danshen and mannitol significantly increased limb survival (P<0.01). Increased limb survival was observed in the SOD-treated group compared with the saline-treated group (P<0.05) but not with the ischemic group

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(P>0.05). Light and transmission electron microscopy revealed that the pathologic and ultrastructural changes in skeletal muscles, which were subjected to 5 h of ischemia followed by 2 h of reperfusion, were reduced with SOD, mannitol and danshen injection. These results suggest that the hydroxyl radical seems to be the most important factor in ischemia and reperfusion injury to skeletal muscle and that danshen, like mannitol, may have a role in antioxidation as a hydroxyl radical scavenger.” “Effect of the extract from salvia miltiorrhiza radix (SMR) on skeletal muscle ischemia and

reperfusion injury in rabbits.” The Canadian Journal of Plastic Surgery 1997, Volume 5,

Number 3: 171-175

14. Radix Angelica Sinensis (Ought-To-Return, Danggui)

A young man went on a long journey to climb a high, treacherous mountain to pick some precious herbs. He told his wife to remarry if he failed to return after 3 years. When he did not return after 3 years, the wife thought he had died climbing the treacherous mountain and eventually she remarried. A few days after she remarried, the husband returned. Upon hearing the news, she burst into tears and became seriously ill. One of the herbs he picked up was a great tonic for women. He decocted the herb and gave it to her to drink. She recovered after a few days. To commemorate the incident, a Chinese poet wrote "He ought to return a little sooner but failed; she ought to wait a little longer but did not." Thus the herb was named "Ought-To-Return". It is also called a "female ginseng". Function of the herb:

! Invigorate blood circulation for the treatment of menstrual disorder ! Promote the flow of vital energy for treatment of abnormal menstruation ! Regulate menstruation due to hormonal imbalance ! Treat headaches and heart disease due to blood stagnation ! Nourish the blood ! For abdominal pain

Clinical studies by western scientists have shown that Radix Angelicae Sinensis has hematopoietic effect (formation of blood cellular components) on person suffering from anemia cause by chronic renal failure. Therefore result showed that Radix Angelicae Sinensis improves kidney functions. The primary cause of anemia of chronic renal failure (CRF) is insufficient production of erythropoietin by the diseased kidneys. Although most patients receiving hemodialysis (HD) respond to recombinant human erythropoietin (rHuEPO) treatment, 4% prove to be rHuEPO resistant. The patient presented in this case study suffered from anemia of CRF that was resistant to rHuEPO therapy. He experienced marked improvement in the anemia after self-initiating regular consumption of the Chinese herb Dang qui (botanical name: Radix angelicae sinensis). The significant hematologic amelioration occurred in the face of a major decrease in the amount of rHuEPO administered. The causes and mechanisms of inadequate response to rHuEPO, as well as the possible beneficial hematopoietic effects of this herbal preparation, are discussed. “Hematopoietic effect of Radix angelicae sinensis in a hemodialysis

patient “Am J Kidney Dis. 1999 Aug; 34(2):349-54. Department of Medicine, Tufts University

School of Nutrition &amp School of Medicine, Boston, MA, USA. PMID: 10430986

Not for for those with hypertension (high blood pressure)


http://www.nutrihuang.com/ErecKing_Testimonials.htm

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24) 40 Plus Tonic

Traditionally used for symptomatic relief of problems related to: 1. Powerful Immune Booster 2. Weak kidneys (lower back ache) 3. Osteoporosis (One of our herbs contains plant-like hormones and behaves like progesterone. It

helps to stimulate the absorption of calcium and our herbs have high colloidal calcium content. One of the herb’s calcium content can be as high as 40,000 ppm. Pure calcium compound like calcium carbonate are not well absorbed by the body)

4. Enhance women’s vitality (Not so tired and exhausted) 5. Increase body warmth (Ideal for those who constantly feel

cold especially at night) 6. Excessive perspiration 7. Tonifies liver, kidney and spleen 8. Improves menstrual period 9. Purifies blood

Herbs:

Poria Cocos Wolf Radix Polygonum Multiflorum Radix Salvia Miltiorrhiza Fructus Lycii Barbarum Chaenomeles Speciosa Cortex Eucommia Ulmoides Folium Perilla Fructescens Sinapis Alba Schizonepeta Tenuifolia Gentiana Macrophylla Cimifugae Rhizome 1) Poria Cocos Wolf:

a) Clinical research by western scientists have shown that Poria cocos improves kidney

functions. 07662 b) Clinical research by western scientists have shown that Poria cocos has anti-inflammatory

functions and protects red blood cells. 160 - 162 c) Clinical studies by western scientists have shown that Poria Cocos Wolf has anti-tumor and

immune-enhancing activity. 65424

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d) Clinical studies by western scientists have shown that triterpene carboxylic acids were

isolated from the methanol extract of Hoelen, Poria cocos, and found to inhibit 12-O-

tetradecanoylphorbol 13-acetate (TAP)-induced edema. 81415 e) Clinical research by western scientists have shown that Poria Cocos have anti-inflammatory

functions, high scavenging activity and protects liver and red blood cells from lipid

peroxidation. 60151

2) Polygonum Multiflorum:

a) Clinical research shows that the extract of the root of Polygonum multiflorum exhibited a

significant antioxidant activity. 33196 b) Clinical research by western scientists have shown that Polygonum Multiflorum increases

high-density lipoprotein (HDL) cholesterol, reduce total cholesterol, free cholesterol,

triglycerides, retard atherosclerosis, protect the heart, strengthen the membranes of

erythrocytes (red blood cells) and has powerful natural antioxidant and free radical scavenger

and these have powerful anti-aging benefits in humans. Forster

3) Salvia Miltiorrhiza:

a) Scientists confirmed the validity of the historical use of the drug to improve heart and

kidneys functions, promoting coronary blood flow without affecting heart rate, inducing

dilation of renal vascular system, an increase in renal blood flow and glomerular filtration rate,

decreases blood urea nitrogen, serum creatinine, methylguanidine, guanidinosuccinic acid and

inorganic phosphate (in rat studies), is a free radical scavenger, inhibits platelet aggregation and

reduces accumulation of methylguanidine and guanidinosuccinic acid levels (methylguanidine

and guanidinosuccinic acid have been reported to cause platelet dynfunction, hemolytic activity,

glucose metabolism disturbance and inhibition of lymphocyte transformation). 1527-0661 b) Clinical research by western scientists confirmed the validity of the historical use of the drug,

Salvia Miltiorrhiza for improving liver functions. Stronger liver would reduce kidney stress.

64749 c) Clinical research by western scientists confirmed the validity of the historical use of the drug,

Salvia Miltiorrhiza for improving liver functions by preventing liver damage and micro-

circulation disorders 41991. d) Clinical research by western scientists confirmed the validity of the historical use of the drug

for improving liver functions. 566-572(7)

4) Fructus Lycii Barbarum: Replenishes vital essence in the liver and the kidneys.

5) Cortex Eucommia Ulmoides: This has the following function:

• Used to replenish liver and kidney • Strengthen bones and muscles • Treats aching back and knees • Tone up liver and kidneys • Strengthen tendon and bones

a) The most interesting study was conducted on an herb called Eucommia. Eucommia contains chemicals that fit into the testosterone receptor and cause it to fire as if testosterone itself were present - causing androgenic, or male-creating changes. These phytoandrogens also increase the activity of real testosterone. The researchers describe a phenomenal synergy between chemicals in Eucommia, testosterone receptors, and testosterone. (see abstract of the study below). Eucommia's value for strengthening the male system is not news to Asian herbalists. The Chinese have been using it for millenia, and they call it Du Zhong. They use it for increasing libido and treating impotence, as well as for some kidney/adrenal complaints, high blood pressure, arthritis and connective tissue diseases. It is one of the 50 main herbs of Traditional Chinese Medicine (TCM), and it has a long history of safe

Page 110

use. The history of searching for plant testosterone is an interesting look into what scientists know, and what they think they know. The scientific literature of the 1950s is littered with failed attempts to find plant sources of testosterone. They all failed because they were looking for a molecule that was identical to human testosterone. No mamalian testosterone was found in plants, but plants do produce some other molecules that hit the testosterone receptors. It took a laboratory 'bioassay' to convince scientists of what the TCM practitioners had been saying all along. The study on Eucommia found that it contains two classes of compounds that pump up Testosterone activity - terpenoids and an unusual type of fatty acids. Therefore it is useful for strengthening weak limps.

b) BACKGROUND: Plants containing compounds such as the isoflavonoids, with female hormone-like effects that bind to human estrogen receptors, are known. But none has been previously shown to have corresponding male hormone-like effects that interact with the human androgen receptor. Here, we report that the tree bark (cortex) of the Gutta-Percha tree Eucommia ulmoides possesses bimodal phytoandrogenic and hormone potentiating effects by lipidic components. METHODS: The extracts of E. ulmoides were tested using in-vitro reporter gene bioassays and in-vivo animal studies. Key compounds responsible for the steroidogenic effects were isolated and identified using solid phase extraction (SPE), high performance liquid chromatography (HPLC), thin layer chromatography (TLC), gas chromatography-mass spectroscopy (GC-MS), electron spray ionisation-mass spectroscopy (ESI-MS) and nuclear magnetic resonance (NMR). RESULTS: The following bioactivities of E. ulmoides were found: (1) a phenomenal tripartite synergism exists between the sex steroid receptors (androgen and estrogen receptors), their cognate steroidal ligands and lipidic augmenters isolated from E. ulmoides, (2) phytoandrogenic activity of E. ulmoides was mediated by plant triterpenoids binding cognately to the androgen receptor (AR) ligand binding domain. CONCLUSION: In addition to well-known phytoestrogens, the existence of phytoandrogens is reported in this study. Furthermore, a form of tripartite synergism between sex steroid receptors, sex hormones and plant-derived lipids is described for the first time. This could have contrasting clinical applications for hypogonadal- and hyperlipidaemic-related disorders. “Novel phytoandrogens and

lipidic augmenters from Eucommia ulmoides”. BMC Complimentary Altern Med 2007 Jan

29;7:3

c) The biologically active compounds and free radical-/ or reactive oxygen species (ROS)-/ scavenging effect of water extract from Du-zhong (WEDZ) were investigated. The WEDZ used included leaves, raw cortex, and roasted cortex. The hot water extract of Du-zhong leaves showed marked activity as a ROS scavenger, and the scavenging effect was concentration dependent. The extract of roasted cortex exhibited a modest scavenging effect on ROS, while the extract of raw cortex had the weakest scavenging effect. The scavenging activity of WEDZ on ROS was correlated to its protocatechuic acid (PCA) content. The content of PCA in Du-zhong determined by HPLC followed the order of leaves (17.17 mg/g) > roasted cortex (2.99 mg/g) > raw cortex (1.16 mg/g). The inhibitory activity of leaf extract of Du-zhong was stronger than that of PCA on the peroxidation of linoleic acid at the same concentration of 0.1 mg/mL. The results presented herein indicated that extract of Du-zhong could possibly. act as a prophylactic (remedy that prevents or slows the course of an illness or disease) agent to prevent free radical-related diseases. “Reactive oxygen species

scavenging activity of Du-zhong (Eucommia ulmoides oliv.) and its active compounds”. J Agric

Food Chem. 2000 Aug;48(8):3431-6.

6) Perilla Frutescens:

a) Clinical research by western scientists have shown that Perilla frutescens has potentially

promising agents for the treatment of allergic diseases like bronchitis and asthma. Research

showed that the anti-allergic titer of rosmarinic acid was more effective than tranilast, which is

a modern anti-allergic drug. Perilla and its constituent rosmarinic acid are potentially

promising agents for the treatment of allergic diseases. 72153 b) Clinical research by western scientists have shown that Perilla frutescens improves lung

functions. In scientists proved that dietary supplementation with perilla seed oil in selected

Page 111

patients with asthma suppresses the generation of LTC4 and is associated with clinical features

such as respiratory function and lipometabolism. 78492 c) Clinical research by western scientists have shown that Perilla frutescens is useful for the

treatment of asthma in terms of suppression of LTB4 and LTC4 generation by leucocytes, and

improvement of pulmonary function. 32825 d) Clinical research by western scientists have shown that Perilla frutescens is an effective

intervention for allergic asthma possibly through the amelioration of increases in cytokines,

chemokines, and allergen-specific antibody. 1365-2222

7) Sinapis Alba:

a) Clinical research by western scientists have shown that allyl and phenyl isothiocyanates

found in Sinapis alba are effective for strengthening the lungs. 81806 b) Clinical research by western scientists have shown that napins (the major allergenic proteins

of Sinapis alba) is useful for building up the body. 66859 8) Radix Gentiana Macrophylla: The function of this herb is to tones up the liver and strengthen bones, tendons and ligaments.

a) Gentiana macrophylla is categorized in the Materia Medica with herbs that expel wind-dampness. The liver is said to nourish the tendons and ligaments and Gentiana macrophylla is particularly used in cases where these connective tissues become hypercontracted due to insufficient nourishment (e.g., deficiency of liver yin), yielding spasms that run from the joints to the muscles. Vangermeersch L

and Sun Peilin, Bi-Syndromes, 1994 SATAS, Brussels, Belgium.

b) Gentiana macrophylla is as often indicated for "bone fever" in TCM pharmacology. This strange term refers to syndromes with fever and sweating in which the person feels as if there is steam coming from the bones, heating up the skin. It is used by herbal scientists to nourish and strenghten bones, temdons and ligaments. State Administration of Traditional Chinese Medicine, Advanced

Textbook on Traditional Chinese Medicine and Pharmacology, (vol. 1) 1995-6 New World

Press, Beijing Overall TCM Pathology

For kidney, liver and spleen function, lower backache, women feeling cold at night, pain of joints & bones due to osteoporosis, purify blood, children with retarded growth, enhance women's vitality and improves functions of the intestines. How does Rena-Liv treat osteoporosis? The herbs stimulate the liver and kidneys in the production of somatomedin. Somatomedin stimulates growth of bone and muscles and promotes cell growth and division and also influences calcium, phosphate, carbohydrate and lipid metabolism. That is why some experiences “puberty” type of sleep at night. It is like turning back the clock and experience a second slow rejuvenation. In TCM, kidneys govern the bones and marrow. Taken long-term it benefits the skin.


http://www.nutrihuang.com/40_Plus_Tonic_Testimonials.htm

Page 112

25) Arthri-T Tonic


1. Arthritic joint problem

Herbs:

Radix Gentiana Macrophylla Cortex Eucommia Ulmoides Radix Notopterygium Incisum Fructus Psoralea Corylifolia Radix Lysimachia Christinae Poria Cocos Wolf 1. Radix Gentiana Macrophylla

Function of the herb: • Antirheumatic agent for treatment of rheumatic pain of the whole body • For allergic inflammation • Tones up the liver

2. Cortex Eucommia Ulmoides: Cortex Eucommia Ulmoides is a type of rubber tree. Historically, it has been commonly used for alleviating lower body pain and for weakness of the sinews and bones. This is a condition that arises with deficiency of the liver and kidney. "Aucubin and the other iridoids of eucommia are likely responsible for the anti-inflammatory effect, which is attained by inhibiting the arachidonic acid pathway" (Recio MC, Structural considerations on the iridoids as anti-

inflammatory agents, Planta Medica 1994; 60(3): 232-234 and Yue H, et al., Development of healthy

beverage from Eucommia ulmoides foliage, Chinese Medicinal Herbs 1999; 22(8): 385-387).This may partly explain its use in treatment of arthritis. Eucommia was mentioned in the Shennong Bencao Jing

"Eucommia is acrid and balanced. It mainly treats pain in the lower back and knees, supplements the center, boosts the essence qi, fortifies the sinews and bones, strengthens the will, and eliminates genital damp itch and dribbling urine after voiding. Protracted taking may make the body light and slow aging".

3. Notopterygium Incisum: Clinical research by western scientists have shown that notopterol,

the analgesic component of Notopterygium incisum, has anti-inflammatory activity by its

inhibitory effect in the vascular permeability test. 39339

Page 113

4. Fructus Psoraleae: Clinical studies by western scientists have shown that Radix Angelicae

Sinensis, Radix Rubiae and Fructus Psoraleae can improve kidney functions. The results before

and after treatment showed an evident difference, with low function before treatment and

normal after treatment. 54885

5. Lysimachia Christinae: Clinical research by western scientists has shown that aqueous

extract of Lysimachia christinae possesses potent hypouricemic (meaning removing uric acid) effects on models of hyperuricemia in mice pretreated with oxonate. 41944

6. Poria Cocos Wolf: a) Clinical research by western scientists from Japan has shown that Poria cocos has anti-

inflammatory functions. 20314 b) Research by western scientists from Spain has shown that Poria cocos is effective in treating

chronic and acute inflammation. 25140 c) Clinical research by western scientists has shown that Poria Cocos Wolf has immune-

enhancing activity. 65424 d) Clinical research by western scientists has shown that Poria cocos has strong anti-tumor

effect (antioxidant). 84472 e) Clinical research by western scientists have shown that Poria Cocos have anti-inflammatory

functions, high scavenging activity and protects liver and red blood cells from lipid

peroxidation. 60151 f) Clinical research by western scientists has shown that Poria cocos has anti-inflammatory

functions and protects red blood cells from AAPH-induced hemolysis (affected by

microorganisms). 160 - 162 g) Clinical research by western scientists has shown that Poria cocos improves kidney functions.

This would protect the kidneys from being damage by uric acid. 07662 Overall TCM Pathology

1) To reduce inflammation

Page 114

26) Kopi Herba Adam


1. Improve and enhance men’s vitality 2. Erectile Dysfunction 3. Strengthen bodily functions 4. Improve men’s .appetite. 5. Remove tiredness after sexual activity 6. Sexual enhancement

Herbs:

Radix Morinda Officinalis Radix Achyranthis Bidentatae Rehmannia Root Cornus Officinalis Ligusticum ChuanXiong Panax Quinquefolium Radix Dipsaci Paeonia Lactiflora For research write-up please go to Adam’s Pride

Page 115

27) Kopi Herba Hawa


1. Menstrual cramps 2. Irregular menstrual period 3. Sallow (pale) complexion 4. Pale lips and finger nails 5. Tonifies, nourishes and promotes blood flow

Herbs:

Radix Angelica Sinensis Radix Paeonia Lactiflora Radix Atractylodes Lancea Radix Glycyrrhiza Glabra Diospycos Kaki L. For research write-up please go to Eve’s Pride

Page 116

28) Teh Herba Cholest-Less


I. High blood pressure 2. High cholesterol 3. Rejuvenates blood vessels (arteriosclerosis) 4. Breathlessness 5. Cold hands and feet, numbness, Circulation problems 6. Protects cardiac muscle from ischemic injury (strengthens the heart) 7. Increases red blood cells production 8. Cancer inhibitive functions 9. Anti-tumor effect 10. Anti-fatigue 1I. Improves immunity 12. Reduces blood fats 13. Antioxidant (protects body against free radicals) 14. Improves brain function Herbs:

Radix Polygonum Multiflorum Radix Saposhnikovia Divaricata Radix Salvia Miltiorrhiza Fructus Schisandra Chinensis For research write-up please go to X-Kolest

Page 117

29) Teh Herba D-B-T

1. Diabetes • Lowers blood sugar • Treats Retinopathy (eye problems) • Treats Neuropathy • Improves the function of liver, spleen, kidney, pancreas

and bladder

Herbs:

Radix Rehmannia Glutinosa Radix Trichosanthis Semen Cuscutae Radix Polygonum Multiflorum Herba Cynomorii Semen Plantaginis Poria Cocos Wolf For research write-up please go to D-Be Te

Page 118

PRODUCTS PROBLEMS - SYMPTOMS TIME TO SEE RESULTS FOODS TO AVOID INITIAL

RESTORATION

EFFECT

DOSAGE

1) 5-Elements

Not for pregnant

women

90 cap

Promotes healthy kidney, liver, spleen,

heart and lungs for the purposes of

Restorative Immunology. Supports

the cardiovascular system, promotes

healthy blood pressure and blood flow

as well as healthy cholesterol level.

Promotes healthy bones and women’s

vitality. Promotes uninterrupted sleep

(for those who suffer from insomnia).

Prevents excessive perspiration.

Increases red blood cell production.

For breathlessness

Depends on the problems being treated.

Generally it is about 10 to 15 days to see

the restorative effect. Takes about 3

months to see a good overhaul of the five

organ system.

Ideal to use as a long-term health

maintenance product

Might feel thirsty,

slight tiredness or

nausea for 2 to 7

days. 1 to 2 weeks

after consumption,

one feels energized.

Initially frequency of

urination increases.

5 to 6 capsules in the

morning

5 to 6 capsules in the

evening

2) X-Kolest

90 cap To improve circulatory system,

strengthen heart, high blood pressure,

high cholesterol, arteriosclerosis,

breathlessness, anti-fatigue, improve

brain function, reduce blood fat, anti-

oxidant

Symptoms - stiff body, mild pain that

seems to run all over the body, tired

easily, poor memory, poor blood

circulation, bad stools, cold hands and

feet

Between 2 weeks to 6 weeks depending

on type of condition

Suitable to take long term for body

maintenance

* See Note below

Feel thirsty, slight

tiredness or nausea

for 2 to 7 days. 1 to

2 weeks after

consumption, one

feels energized and

for those who suffer

from breathlessness,

the problem stops

3 capsules in the

morning

3 capsules in the

evening

3) Rener-Liv

Not for pregnant

women

90 cap

For kidney, liver and spleen function -

lower back pain, women feeling cold

at night, pigmentation, pain of joints &

bones due to osteoporosis, purifies

blood, children with retarded growth,

enhance women's vitality

Symptoms - lower backache

1 to 3 months

(suitable to take long term for body

maintenance)

* See Note below


maintenance

Citrus fruit drinks,

barbecued foods Feel thirsty and body

feels warm. May

need to sleep early

because bone is been

nourished

3 capsules in the

morning

3 capsules in the

evening

Page 119


RESTORATION

EFFECT

DOSAGE

4) Breath-Ezy

90 cap For weak lung

Symptoms - frequent bouts of colds

and flu, lethargy, excessive

perspiration

Minimum 2 weeks depend on the age

group.


maintenance

Cold drinks, fruit

juice, cold Chinese

tea

Initially feels thirsty

and increased

frequency of

urination and body

feels warm. For

some there will be

coughing out of

phlegm

3 capsules in the

morning

3 capsules in the

evening

5) D-Be-Te

60 cap Lower blood sugar, treats retinopathy,

improve functions of liver, kidneys,

spleen, pancreas, bladder and heart

2 weeks

Excessive sugar and

oily foods 2 capsules in the

morning

2 capsules in the

evening 6) X-Gas-3

60 cap Gastric problems (the only product in

the world that effectively cure gastric

all the time)

1 to 2 weeks Cold drinks, cold

fruit juice, cold fruits

and must consume 3

regular meals a day

4 capsules in the

morning

7) Adam’s Pride

60 cap Erectile Dysfunction (ED)

Enhance libido For total ED - minimum 3 months

For enhancement - 10 to 15 days Have extra energy

and more alert

2 capsules in the

morning

2 capsules in the

evening 8) Eve’s Pride

60 cap

Menstrual cramps and irregular

menstrual period

Symptoms - pale face, lethargy

Approximately 2 weeks Citrus fruit drinks 2 capsules in the

morning

2 capsules in the

evening 9) Alco-Liv

90 cap For liver cirrhosis, liver detoxification,

fatigue due to poor liver function,

expels urinary stones, fatty liver.

Symptoms for this type of liver

problems – always feeling thirsty

Minimum 1 month Alcohol Improved appetite 3 capsules in the

morning

3 capsules in the

evening

10) ColonEase

60 cap

For intestinal and colon cleansing The next day Initially the stools

will be loose, sticky

and smelly.

Frequency of

defecation may

increase.

6 to 8 capsules before

bed or in the morning

before breakfast

Page 120


RESTORATION

EFFECT

DOSAGE

11) Uri-X

90 cap Gout based problems - high uric acid 2 weeks Any food that

increases uric acid

levels

3 capsules in the

morning

3 capsules in the

evening 12) NerboneCare

60 cap Weak joints - to nourish tendons,

ligaments, nerves and bones 1 week to 1 month Certain part of the

body may feel

painful for 10 to 14

days because of

restoration crises.

2 capsules in the

morning

2 capsules in the

evening

13) Steve-Nek

60 cap

For stiff neck and lower backache due

to poor sitting position

2 to 4 days 2 capsules in the

morning

2 capsules in the

evening

14) Vira-Liv

60 cap

For problems related to hepatitis 1 month Avoid sea foods like

cockles and all types

of shellfish

2 capsules in the

morning

2 capsules in the

evening

15) Stro-Care

90 cap

For stroke Minimum 9 months to a year 4 capsules in the

morning

4 capsules in the

afternoon

4 capsules in the

evening

16) InfaLive

60 cap For weak children

Symptoms - always sick, prominent

green veins on the face

Minimum 1 month Cold drinks or cold

fruit juice, cold fruits Improved appetite 2 capsules in the

morning

2 capsules in the

evening 17) Warm-Rener

90 cap

For lower backache, constant

urination, uncontrollable urination

1 month "Kat Chai" drinks,

barbecued foods 3 capsules in the

morning

3 capsules in the

evening

Page 121


RESTORATION

EFFECT

DOSAGE

18) SpitalCare

90 cap Always spit out sticky whitish phlegm

(although do not have flu or cold) Minimum 2 weeks Cold drinks or cold

fruit juice, cold fruits There will be

coughing out of

phlegm

3 capsules in the

morning

3 capsules in the

evening 19) Slym-Care

90 cap

For weight loss Minimum 1 month Avoid over eating Body feels very

warm because of

lipolysis

3 capsules in the

morning

3 capsules in the

evening

20) Run-On

90 cap

For weak legs and arms Minimum 1 month 3 capsules in the

morning

3 capsules in the

evening

21) ErecKing

50 ml For men with ED and weak erection

problems

For ladies: consume this product with

EVE’S PRIDE

Usually within 5 days to 7 days Stomach feels very

warm with 10

minutes and slowly

spread to the rest of

the body

5 to 10 ml before bed

22) 40 Plus Tonic

50 ml

For men and women above 40 Usually within 5 days to 14 days Body feels very

warm the whole

night

10 to 15 ml before

bed

23) P 21

15 ml Diabetic foot ulcer, bed sores, cold

sores, sore throat, rashes, burns,

sinusitis, etc .

Spray or use cotton

bud to apply

24) D T J

30 ml For nerve, tendon and ligament injury Application period: 4 to 8 days Rub affected area

until warm. Rub in

D T J. Do not

expose affected area

to water for 2 to 3

hours

25) Arthri-T Tonic

100 ml

Arthritis joint problem One month High uric acid food 5 to 10 ml before bed

For children aged below 12 - half dosage.

Page 122

As our general body health conditions are different, it is advisable to start with half dosage and gradually increases to full dosage especially for products like RespireCare,

Rena-Liv and X-Cholest. If it is taken for maintenance, every three months, stop for one month.

Please take note:

1. Rena-Liv – For those who consistently have poor appetite, this product will bring the appetite back to normal but for those with normal appetite, it would not

increase their appetite further.

2. X-Cholest – For those who take cholesterol-lowering drugs, it affects the efficacy of X-Cholest

NOTE: As all our health conditions are different, different individual may experience different results.

Page 123

Product Usage Guide by Disease Problems Holistic Relieve and Rejuvenation

1 Amnesia 40 PLUS TONIC

2 Anaemia

Micro: X-KOLEST and RENER-LIV

Macro: SPITALCARE, 40 PLUS TONIC and X-KOLEST

3 Angina X-KOLEST, ALCO-LIV and NERBONECARE

4 Anorexia SPITALCARE, X-GAS-3, ALCO-LIV and COLONEASE

5 Anti-Aging SPITALCARE, X-KOLEST, 40 PLUS TONIC

6 Arteriosclerosis X-KOLEST

7 Arthritis ARTHRI-T TONIC, 40 PLUS TONIC, RENER-LIV and D T

J

8 Bed Sores P 21

9 Bladder Problems Incontinence: WARM-RENER. Weak Kidneys: RENER-LIV or

40 PLUS TONIC

10 Blocked Nose P 21

11 Breathlessness X-KOLEST and NERBONECARE

12 Bronchial Asthma Micro Holistic: BREATH-EZY

Macro Holistic: BREATH-EZY, SPITALCARE and 40 PLUS

TONIC

13 Burns P 21 (BEFORE BLISTER IS FORMED, QUICKLY SPRAY

P21 ONTO THE BURN AREA EVERY 30 SECONDS FOR 7

TO 8 TIMES)

14 Cardiac Insufficiency X-KOLEST and NERBONECARE

15 Cataracts X-KOLEST, ALCO-LIV and 40 PLUS TONIC

16 Cholesterol X-KOLEST (IF YOU HAVE FATTY LIVER PROBLEMS

THEN ALCO-LIV SHOULD BE ADDED)

17 Chronic Bronchitis SPITALCARE and BREATH-EZY

18 Cold Sores P 21

19 Colon Cleanse COLONEASE

20 Constipation COLONEASE, SPITALCARE and 40 PLUS TONIC

21 Cough (Prolong) SPITALCARE, BREATH-EZY and ALCO-LIV

22 Deafness 40 PLUS TONIC and RENER-LIV

23 Diabetes Micro Holistic: D-BE-TE

Macro Holistic: D-BE-TE, SPITALCARE, X-GAS-3 and 40

PLUS TONIC

24 Diabetic Ulcer P 21

25 Dizziness X-KOLEST, ALCO-LIV, SPITALCARE and 40 PLUS

TONIC

26 Eczema Micro Holistic: P 21

Macro Holistic: P 21, BREATH-EZY, COLONEASE, X-GAS-

3, X-KOLEST

27 Edema (Heart) X-KOLEST

28 Edema (Kidney) RENER-LIV

29 Emphysema BREATH-EZY, X-KOLEST and 40 PLUS TONIC.

30 Energy Booster 5-ELEMENTS and 40 PLUS TONIC

31 Enuresis WARM-RENER

32 Epilepsy SPITALCARE, BREATH-EZY, X-KOLEST, 40 PLUS

TONIC, and NERBONECARE

33 Erectile Dysfunction Micro Holistic: ADAM’S PRIDE or ERECKING

Macro Holistic: ADAM’S PRIDE or ERECKING and

SPITALCARE, X-KOLEST ALCO-LIV, 40 PLUS TONIC

and BREATH-EZY

Page 124

34 Exhaustion X-KOLEST, ALCO-LIV, SPITALCARE and 40 PLUS

TONIC

35 Eyesight X-KOLEST, ALCO-LIV and NERBONECARE

36 Fatty Liver ALCO-LIV

37 Flatulence SPITALCARE, X-GAS-3 and INFALIVE

38 Folliculitis P21

39 Forgetfulness 40 PLUS TONIC, ALCO-LIV and SPITALCARE

40 Frozen Shoulder SPITALCARE, BREATH-EZY, 40 PLUS TONIC,

NERBONECARE and D T J.

41 Gallstones Use one whole lemon to squeeze juice and mix juice with slightly

hot water. Drink this combination 8 times a day at an interval of 1

hour for 14 days continually without break and then take ALCO-

LIV

42 Gastritis X-GAS-3

43 Gastric Ulcer SPITALCARE, X-GAS-3, and ALCO-LIV

44 Genital Rashes P 21

45 Gingivitis P21

46 Glaucoma X-KOLEST, SPITALCARE and RENER-LIV

47 Gout Micro Holistic: URI-X

Macro Holistic: URI-X, X-KOLEST, ALCO-LIV, and 40

PLUS TONIC.

48 Hay Fever SPITALCARE, BREATH-EZY and P 21

49 Headache ALCO-LIV and X-GAS-3

50 Heartburn SPITALCARE, X-GAS-3, and ALCO-LIV

51 Hepatomegaly

(Alcohol)

ALCO-LIV

52 Hepatomegaly

(Virus)

VIRA-LIV

53 Hepatitis VIRA-LIV

54 Hiatus Hernia SPITALCARE and X-GAS-3

55 Hot Flashes EVE’S PRIDE AND 40 PLUS TONIC.

56 Hypertension X-KOLEST, STRO-CARE, ALCO-LIV (NERBONECARE

SPITALCARE and 40 PLUS TONIC (or RENER-LIV)

OPTIONAL)

57 Impotence Adam’s Pride or ErecKing

58 Incontinence WARM-RENER

59 Infected Wound P 21

60 Infected ear P 21

61 Insomnia 5-ELEMENTS

62 Irregular

Menstruation

EVE’S PRIDE

63 Irritable Bowel

Syndrome

SPITALCARE, X-GAS-3 and RENER-LIV

64 Ladies’ Libido EVE’S PRIDE, ERECKING

65 Lethargy 5-ELEMENTS

66 Leukorrhoea P21

67 Ligaments Problems D T J, NERBONECARE and X-KOLEST

68 Liver Cirrhosis

(Alcohol)

ALCO-LIV

69 Liver Cirrhosis

(Virus)

VIRA-LIV

70 Liver Detox (Alco) ALCO-LIV

71 Liver Detox (Hepa) VIRA-LIV

Page 125

72 Lower Backache RENER-LIV or WARM-RENER

73 Lumbago STEVE-NEK, X-KOLEST and 40 PLUS TONIC

74 Menopause EVE’S PRIDE

75 Menstrual Cramps EVE’S PRIDE

76 Migraine X-KOLEST, ALCO-LIV, SPITALCARE and X-GAS-3

77 Mouth Ulcer P 21

78 Multiple Sclerosis X-KOLEST NERBONECARE STRO-CARE and 40 PLUS

TONIC

79 Muscle Stress/Ache STEVE-NEK

80 Nephritis BREATH-EZY, SPITALCARE and 40 PLUS TONIC

81 Neuralgia ALCO-LIV

82 Neuropathy D-BE-TE and NERBONECARE

83 Nose and Troat

Aliments

BREATH-EZY and SPITALCARE.

84 Old Age Bones and

Joints

NERBONECARE

85 Osteoarthritis Micro Holistic: NERBONECARE

Macro Holistic: NERBONECARE, 40 PLUS TONIC, ALCO-

LIV and X-KOLEST

86 Osteoporosis Slow: RENER-LIV

Fast: 40 PLUS TONIC and RENER-LIV

87 Ovulation Prob. X-KOLEST, ALCO-LIV and 40 PLUS TONIC

88 Palpitations X-KOLEST, NERBONECARE, ALCO-LIV, SPITALCARE

and 40 PLUS TONIC

89 Peptic Ulcer X-KOLEST, SPITALCARE, X-GAS-3 and ALCO-LIV

90 Pigmentation SPITALCARE and VIRA-LIV

91 Pimples P 21

92 Poor Appetite (Adult) INFALIVE and RENER-LIV

93 Poor Appetite INFALIVE and RENER-LIV

94 Poor Blood

Circulation

X-KOLEST

95 Prolapse SPITALCARE (has to be taken long-term)

96 Psoriasis Relieve: SPITALCARE, ALCO-LIV and RENER-LIV

Application: P 21

97 Rashes P21

98 Retinopathy D-BE-TE

99 Restlessness X-KOLEST, SPITALCARE and 40 PLUS TONIC

100 Rheumatoid Arthritis Detox: URI-X

Nourishment: 40 PLUS TONIC, ALCO-LIV, SPITALCARE

and RESPIRECARE.

101 Sagging Bosom SPITALCARE

102 Schizophrenia X-KOLEST, 40 PLUS TONIC and ALCO-LIV

103 Sciatica X-KOLEST, ALCO-LIV and RENER-LIV

104 Sinusitis P21, BREATH-EZY, ALCO-LIV and SPITALCARE

105 Skin Rashes P 21

106 Sore Throat P 21

107 Sperm Problems ADAM’S PRIDE or ERECKING and 40 PLUS TONIC

108 Spleen Detox SPITALCARE

109 Sprain D T J

110 Stiff Neck STEVE-NEK

111 Stings P21

112 Stroke NERBONECARE, X-KOLEST, ALCO-LIV, STRO-CARE

113 Sweating (Excessive) BREATH-EZY and SPITALCARE

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114 Teeth Grinding SPITALCARE

115 Tendon disorder ALCO-LIV, SPITALCARE, X-KOLEST and

NERBONECARE

116 Tennis Elbow

117 Tic ALCO-LIV, SPITALCARE and X-KOLEST

118 Tinnitus 40 PLUS TONIC, X-KOLEST and NERBONECARE

119 Toothaches P21

120 Tonsillitis 40 PLUS TONIC and BREATH-EZY

121 Uric Acid Micro Holistic: URI-X

Macro Holistic: URI-X, X-KOLEST, ALCO-LIV, and 40

PLUS TONIC.

122 Uterine disorders EVE’S PRIDE, SPITALCARE, BREATH-EZY and 40 PLUS

TONIC

123 Vertigo X-KOLEST, ALCO-LIV, NERBONECARE and BREATH-

EZY.

124 Weak Children INFALIVE

125 Weak Constituent 40 PLUS TONIC.

126 Weak Erection Micro Holistic: ADAM’S PRIDE or ERECKING

Macro Holistic: ADAM’S PRIDE or ERECKING and

SPITALCARE, X-KOLEST ALCO-LIV and 40 PLUS TONIC

127 Weak Leg RUN ON

128 Weak Lungs BREATH-EZY and SPITALCARE (with phlegm)

BREATH-EZY (without phlegm)

129 Weight Loss Detox: COLONEASE

Lipolysis: SLYM-CARE

Restore: 40 PLUS TONIC, ALCO-LIV and SPITALCARE

130 White Sputum SPITALCARE and BREATH-EZY

Products you can trust.

For more information kindly go to: http://www.nutrihuang.com/Treatment_Pathology.htm

or contact any Awal Mart representative. Thank you

Advice: Do not trust fanciful concept or symptomatic relief that only masks your problems. Go

for the real thing!!!

Please note the change in name: RespireCare to Breath-Ezy, IntestCleanse to Colonease, Diabe-

X to D-Be-Te, LadyCare to Eve’s Pride, Rena-Liv to Rener-Liv, Hour Glass to Slym-Care,

Cold-Rener to Warm-Rener, X-Hepa to Vira-Liv and X-Cholest to X-Kolest

Page 127

Understanding How you are Healed

Proven Macro Holistic TCM Treatment Pathology

1. ACNE (PIMPLES) Description: An inflammatory disease cause by problematic sebaceous glands. Cause: Spleen and stomach deficiency, occasionally the trouble can be traced to the lungs.

Also damp heat brought about by prolonged overeating of hot and damp food causing

unbalance in the digestive system. Treatment: 1. Micro Holistic: Treat the skin infection and treat the inflammation

2. Macro Holistic: Restore functions of the spleen, the stomach, and the lungs as well

as detoxify the large and small intestines. Products to 1. Micro Holistic: P 21 Relieve: 2. Macro Holistic: P 21, SPITALCARE, X-GAS-3, BREATH-EZY

and COLONEASE. Restriction: To avoid deep-fried food like fish and chips or crisps, or hot food like chilly and

curry. Also to avoid alcohol and to cut-down on dairy products especially chocolates.

Do not apply oils and lotions as both will only make matters worse.

2. AMNESIA Description: Temporary memory loss can be caused by severe shock often following an accident,

and sometimes illness. Meningitis, epilepsy or brain tumour can trigger amnesia. Cause: Kidney weakness due to lack of “essence”. Treatment: To tonify and strengthen the kidneys so that it can nourish the brain to improve

memory. "Kidneys nourish the marrow and marrow feeds the brain". Products to

Relieve: 40 PLUS TONIC Restriction: Avoid coffee, Chinese tea, fried foods, fatty foods, dairy products, cold drinks and

carbonated drinks. 3. ANAEMIA Description: Persons with a sallow complexion due to iron deficiency. Cause: Spleen not transforming qi correctly. Pernicious anaemia is caused by the body's

failure to absorb adequate quantities of vitamin B12, and it can affect vegans (a strict

vegetarian) whose diet may lack protein. Megaloblastic anaemia is caused by a

deficiency of folic acid found in fresh vegetables and liver. Treatment: 1. Micro Holistic: Induce the body to increase blood production

2. Macro Holistic: Restore functions of the spleen and kidneys and induce blood

production. Products to 1. Micro Holistic: X-KOLEST and RENER-LIV Relieve: 2. Macro Holistic: SPITALCARE, 40 PLUS TONIC and X-KOLEST Restriction: Avoid consuming iron supplements to prevent constipation. Drink plenty of water.

4. ANGINA Description: Inadequate supply of blood to the heart which impedes the blood flow through a

narrowing of the arteries. It usually comes on suddenly, causing severe chest pains,

often spreading to one or both arms and to the neck, throat, jawbone or back. Cause: Hardening and narrowing of the arteries due to accumulation of cholesterol. The

cholesterol plaque causes narrowing of the coronary arteries which leads to reduce

oxygen supply to the heart Treatment: Thin blood down, reduce cholesterol and triglyceride levels, which are contributory

factors in atherosclerotic heart disease and dislodge the blockage in arteries, as well

as softening of the blood vessels. Products to

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Relieve: X-KOLEST, ALCO-LIV and NERBONECARE Restriction: Avoid fried, barbeque and fatty foods and alcoholic drinks, Chinese tea, dairy

products. 5. ANOREXIA Description: Often called the "slimmer's disease" because it is a phobia against eating and a

fixation about weight gain. Cause: A weakness in the digestive system, the intestines and the spleen. Treatment: To restore the digestive system, the intestines and the spleen. Products to

Relieve: SPITALCARE, X-GAS-3, ALCO-LIV and COLONEASE Restriction: Avoid coffee, Chinese tea, fast foods, dairy products, starchy foods, and snacks.

6. ARTERIOSCLEROSIS Description: Hardening of the arteries. Cause: An ageing process when the arterial walls lose tone, and the blood circulation is

affected. Symptoms include spells of dizziness, general weakness of blurred vision,

and in severe cases there can be a risk of thrombosis or stroke. Symptoms Spells of dizziness and body aches at different location at different times. Treatment: 1. Micro Holistic: Treat the plaque and cholesterol problem and soften the blood

vessel 2. Macro Holistic: Two major contributory factors are the blood vessels and the

blood itself. The heart, kidneys, spleen and liver are all involved in the quality of the

blood Products to 1. Micro Holistic: X-KOLEST and NERBONECARE Relieve: 2. Macro Holistic: X-KOLEST, NERBONECARE, SPITALCARE and

ALCO-LIV Restriction: Avoid fried, barbeque and fatty foods and alcoholic drinks, Chinese tea, dairy

products.

7. ARTHRITIS Description: Forms of arthritis are osteoarthritis and rheumatoid arthritis. Osteoarthritis usually

affects the hips, knees, spine and hands, causing them to swell and stiffen. The

condition is caused when the cartilage between the bones of the joints becomes worn;

so that the bones rub against each other when the joints are flexed, causing intense

pain. Rheumatoid arthritis also involves swelling and stiffness and pain in the joints.

It is characterized by feverish attacks during which the tissue, tendons and ligaments

of the joints become inflamed and the skin swells and becomes very tender. Cause: Both kinds of arthritis are caused by "wind damp"; one is the result of wind cold and

the other of wind heat. In cold arthritis the joints are painful particularly in the cold

weather whereas when heat is the cause of arthritis, there is swelling and heat in the

joints. Treatment: To clear the wind and dampness so as to release the energy that has become

congealed. Products to

Relieve: ARTHRIT TONIC, 40 PLUS TONIC, RENER-LIV and D T J Restriction: Avoid Chinese tea, coffee, continual strain on joints, continual soaking of joints in

water and bathing after sunset.

8. BACK PAIN Description: Variable pains arising from either one of these problems; muscular, spinal, and

nervous in origin or caused by a disease of the internal organs, strained or weak

muscles or sciatica. Cause: Pending on diagnosis the cause of pain maybe due to strained nerves and muscles,

compressed nerves, weak kidneys, and poor blood circulation.

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Treatment: To improve blood circulation and treat the strained muscles, repair nerves and to

nourish the kidneys. Products to 1. Strained nerves and muscles: STEVE-NEK Relieve: 2. Compressed nerves: D T J

3. Weak kidneys: 40 PLUS TONIC and RENER-LIV Restriction: For problems caused by weak kidneys: Avoid pickled foods, cold drinks.

9. BALDNESS Description: Baldness is often due to hereditary factors and as such is difficult to treat. Cause: The Chinese theory is that hair is nourished by the blood (which is dominated by the

liver) and by the essence of the kidney. Treatment: Liver and kidney deficiencies are considered the likely causes of baldness, so

treatment will involve toning both the organs. Products to

Relieve: X-KOLEST, ALCO-LIV, 40 PLUS TONIC Restriction: Avoid lesser salt intake and prolonged sleep defiant

10. BLADDER PROBLEMS Description: A weak bladder causing urination at frequent intervals and also a leaking bladder that

cannot hold urine for long. Cause: Due to weakness of the kidney qi or the kidney yang. If the kidneys are weak, the

quality of the urine is poor, and this irritated the bladder. Also known as “Kidney

Flu” in the medulla part of the kidneys. Treatment: To warm and strengthen the kidneys. Products to 1. WARM-RENER (if due to incontinence: “kidney flu”) Relieve: 2. 40 PLUS TONIC (no incontinence: weak kidneys) Restriction: Avoid cold drinks, Chinese tea and cold desserts.

11. BREATHLESSNESS Description: A person who suffers from the shortness of breath. Cause: The accumulation of cholesterol plaque causes narrowing of the blood vessels and the

poor blood flow caused by accumulation of blood fat which leads to reduce oxygen

supply to the body Treatment: Treat the plaque and cholesterol problem and reduce blood fat Products to

Relieve: X-KOLEST and NERBONECARE Restriction: Avoid fried, barbeque and fatty foods and alcoholic drinks, Chinese tea, dairy

products.

12. BRONCHIAL ASTHMA Description: A deficiency of the lungs energy that fails to clear the air passages resulting in

breathing difficulty. Cause: The major cause is phlegm produced by a weakness of lungs, spleen and kidneys

resulting in phlegm clogging the lungs. Treatment: 1. Micro Holistic: To strengthen the lungs

2. Macro Holistic: To nourish and to restore the functions of the spleen, the kidneys

and the lungs to dispel phlegm. Products to 1. Micro Holistic: BREATH-EZY Relieve: 2. Macro Holistic: BREATH-EZY, SPITALCARE and 40 PLUS TONIC Restriction: Avoid cold drinks, Chinese tea, smoking areas, dairy products especially chocolate,

milk and sweets

13. CATARACTS Description: Clouding of the natural lens of the eye impairing vision. Cause: Weakness of the liver and kidneys due to the lack of blood nourishment.

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Treatment: To nourish the blood, the liver and the kidneys. Products to

Relieve: X-KOLEST, ALCO-LIV and 40 PLUS TONIC

Restriction: Avoid coffee, Chinese tea, pickled foods, fried and fatty foods, dairy products, and

direct sunlight.

14. CHRONIC BRONCHITIS Description: Chronic coughing due to external reasons such as wind, cold or heat invasion and is

attributed to lung problems. Cause: Deficiency of the spleen and lungs resulting in a built-up of phlegm. Treatment: To tonify and restore spleen and lungs interactivity relationship. Products to

Relieve: SPITALCARE and BREATH-EZY Restriction: Avoid cold drinks, Chinese tea, smoking areas, dairy products especially chocolate,

milk and sweets and bathing after sunset.

15. CONSTIPATION Description: Constipation is defined as having a bowel movement fewer than three times per

week. Cause: The spleen plays an important part in digestion and stool production through

its transformation and transportation functions. These functions need the

support of the kidneys to perform normally. When kidney yin is deficient, the

large intestine does not have adequate fluid for nourishment and constipation results. Therefore the cause is kidney yin deficiency, excessive heat in the large

intestine and spleen deficiency. Treatment: 1. Micro Holistic: Cool the intestines

2. Macro Holistic: Tonify the kidneys and strengthen the spleen and cool the

intestine Products to 1. Micro Holistic: COLONEASE Relieve: 2. Macro Holistic: COLONEASE, SPITALCARE and 40 PLUS TONIC Restriction: Avoid cold drinks and fruits and sour foods, Chinese tea.

16. DEAFNESS Description: Partial or complete loss of hearing or tinnitus caused by age or excessive mucus and

wax formed in the years. Cause: Weakness in the kidneys. Treatment: To tonify and nourish the kidneys. Products to

Relieve: 40 PLUS TONIC and RENER-LIV Restriction: Avoid Chinese tea, coffee and cold drinks.

17. DIABETES (see also RETINOPATHY) Description: When the body becomes insulin tolerant or when the pancreas is unable to produce

enough insulin to regulate the body’s blood sugar level. Cause: The problem is caused by excessive thirst in the upper abdomen and excessive

urination in the lower abdomen and the failure of the digestive system to regulate

glucose. Failure of the digestive system to regulate glucose. Primarily considered a

problem related to the stomach, the kidneys and the spleen. In addition lungs are

having a functional problem caused by heat in the body through a yin deficiency. Treatment: The principle treatment for diabetes is to treat and strengthen the upper, middle and

lower abdomen by clearing the heat from lungs stomach and kidneys and to nourish

the spleen, stomach and kidneys at the same time. Products to 1. Micro Holistic: D-BE-TE Relieve: 2. Macro Holistic: D-BE-TE, SPITALCARE, X-GAS-3 and 40 PLUS

TONIC

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(Note: If diabetes has affected the blood circulatory system then products for treating

blood circulatory system should be taken) Restriction: Avoid excessive sweetened foods and fruits, Chinese tea, food rich in carbohydrate,

alcoholic and carbonated drinks.

18. DIZZINESS Description: The inability to feel balance and stay focus in vision and having a reeling sensation. Cause: Liver heat rising due to kidney deficiency, blood deficiency and an invasion of wind

due to pathogenic factors. Treatment: To tonify the liver, nourish the kidneys and nourish the blood. Products to

Relieve: X-KOLEST, ALCO-LIV, SPITALCARE and 40 PLUS TONIC Restriction: Avoid fried and fatty foods, hot and spicy foods, dairy products, Chinese tea and

pickled foods. 19. ECZEMA Description: There are many different types of this skin disease; (1) weeping skin with discharge

and sensation of itchiness, (2) skin dryness, redness and itchiness, (3) skin eruptions. Cause: Deficiencies of the lungs, stomach, heart and blood. (1) damp heat of the organs, (2)

heat in the blood, (3) wind attacks the organs Treatment: 1. Micro Holistic: Sooth the skin problems

2. Macro Holistic: To clear toxins in the lungs, spleen, stomach and blood and to

restore functions of the organs and sooth the skin problem Products to 1. Micro Holistic: P 21 Relieve: 2. Macro Holistic: P 21, BREATH-EZY, COLONEASE

X-GAS-3, X-KOLEST Restriction: Avoid fried and fatty foods, hot and spicy foods, dairy products, pickled foods, chilly

and spices, salted foods, Chinese tea, and seafood.

20. EMPHYSEMA Description: Mucus blocking the air passages in the lungs leading to chronic bronchitis and poor

blood circulation within the lungs. Cause: Continual exposure to smoking, pollutants and asthmatics causing the elasticity of the

lungs to be impaired and having poor blood circulation affecting respiration. Treatment: To strengthen the lungs along with the heart and kidneys. Products to

Relieve: BREATH-EZY, X-KOLEST and 40 PLUS TONIC. Restriction: Avoid smoking and smoking areas, alcoholic drinks, spicy foods, dairy products and

Chinese tea.

21. ENURESIS (BED-WETTING) Description: The inability to hold urine. Cause: Weak kidneys leading to functional disorder of kidney energy to control bladder and

to hold urine. Treatment: To warm-up the kidneys and to strengthen kidney energy. Products to

Relieve: WARM-RENER Restriction: Avoid cold drinks, Chinese tea.

22. EPILEPSY Description: Excessive electrical discharges in some of the nerve cells in the brain causing

momentarily loss of consciousness froth at the mouth and threshing of the hands and

arms while the eyes blink rapidly. Cause: Phlegm blocking the orifice of the heart, plus internal dampness and qi or blood

stagnation.

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Treatment: To strengthen the functions of the spleen, the lungs, warm-up the spleens, nourish the

kidneys and improve quality of blood, and to remove blood stagnation. Products to SPITALCARE, BREATH-EZY, X-KOLEST, 40 PLUS TONIC, and

Relieve: NERBONECARE. Restriction: Avoid cold drinks, smoking areas, coffee, Chinese tea, dairy products, bath after

sunset, fried and fatty foods, hot and spicy foods.

23. EXHAUSTION Description: Energy deficiency leading to easy tiredness even at the slightest use of physical or

mental energy. Cause: A qi and blood deficiency Treatment: To nourish the liver and kidneys, and to restore blood circulation. Products to

Releive: X-KOLEST, ALCO-LIV, SPITALCARE and 40 PLUS TONIC Restriction: Avoid Chinese tea, and fried foods.

24. EYESIGHT Description: Problems include vision impaired and sight easily blurred due to eye straining in

artificial light and poor natural light for long hours. Cause: Blood deficiency and poor blood circulation causing eye muscles to weaken and

under nourished. Treatment: To nourish the blood and remove plaque in blood vessels. Products to

Relieve: X-KOLEST, ALCO-LIV and NERBONECARE Restriction: Avoid Chinese tea, alcoholic drinks, and fried foods.

25. FLATULENCE Description: A state of excessive gas in the alimentary canal. Cause: Food stagnation and damp heat or indigestion caused by stagnation of the stomach

energy. Treatment: To tonify spleen and stomach functions and to dispel heat in stomach. Products to

Relieve: SPITALCARE, X-GAS-3 and INFALIVE Restriction: Avoid oily foods, fried foods, icy drinks, beverages, dairy products, hot and spicy

foods, yellow noodles (mee) and nuts and beans of all kinds.

26. FORGETFULNESS/ POOR MEMORY Description: Poor memory usually age-related to remember names or telephone numbers and

events. Cause: Deficiency of the kidney essence, spleen and liver qi Treatment: Tone and nourish the kidneys Products to 40 PLUS TONIC, ALCO-LIV and SPITALCARE if other general weaknesses

Relieve: are present. Restriction: Avoid Chinese tea, pickled foods, salted foods, and cold drinks.

27. FROZEN SHOULDER Description: Water in the body congeals into dampness thereafter stagnates in the shoulder joint.

This usually starts with inflammation of the tendons in the joint, or inflammation of

the joint itself. Cause: Weakening of yang qi Treatment: To nourish the spleen, lungs, kidneys and to remove plaques in nerves and to tonify

tendons. Products to SPITALCARE, BREATH-EZY, 40 PLUS TONIC, NERBONECARE and

Relieve: D-T J.

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Restriction: Avoid cold drinks, Chinese tea, bath after sunset, pickled foods, seafood, sour foods,

ducks, fried foods, and salted foodstuff.

28. GALLSTONES Description: The symptoms include pain in the upper abdomen and tenderness under the ribs on

the right side of the body, accompanied by nausea and vomiting. Cause: Damp heat in the liver and gallbladder Treatment: To remove heat in the liver and to dissolve stones in the gallbladder. Use one whole

lemon to squeeze juice and mix juice with slightly hot water. Drink this combination

8 times a day at an interval of 1 hour for 14 days continually without break. Products to

Relieve: ALCO-LIV. Restriction: Avoid coffee, Chinese tea, beverage drinks, carbonated drinks, fried and fatty foods,

hot and spicy foods, curry of any sort, and sour foods.

29. GASTRIC ULCER Description: The protective mucus coating in the stomach has worn off due to regurgitation of bile

or over-production of acid. Cause: Ulcer is caused by stomach qi and liver qi stagnation. Food allergies, stress, certain

drugs and poor eating-habits, heavy drinking or smoking are the major sources of

ulcer. Treatment: The move the stomach qi and liver qi. Products to

Relieve: SPITALCARE, X-GAS-3, and ALCO-LIV. Restriction: Avoid smoking areas, alcoholic drinks, Chinese tea, dairy products, hot and spicy

foods, sour foodstuff, pickled foods, carbonated drinks, salted foodstuff. Eat at least

three meals daily.

30. GLAUCOMA Description: Blurred vision as the result of build-up of fluid in the area between the cornea and the

lens of the eye due to blockage of the channel or the tissues through which fluid

drains. This increases pressure on the eyeball and affecting tiny blood vessels at the

back of the eye where nerve fibers from the retina enter the optic nerve. Cause: Weakness in the liver and kidneys combined with heat and fire in the gallbladder,

stomach and the liver. Treatment: To strengthen the kidneys and liver thereby reducing the fire (heat) and clearing the

phlegm. Products to

Relieve: X-KOLEST, SPITALCARE and RENER-LIV Restriction: Avoid Chinese tea, cold drinks, carbonated drinks, fried foods

31. GOUT Description: An excess of uric acid and other compounds which accumulate in the blood. The

intense pain and swelling in the joints is caused by acid and urate crystals and the

crystals can also collect in the kidneys to cause kidney stones. Cause: Heat and damp in the blood and liver channel and kidney deficiency. Treatment: 1. Micro Holistic: To remove toxic excess

2. Macro Holistic: To remove toxic excess and treat the underlying problems Products to 1. Micro Holistic: URI-X Relieve: 2. Macro Holistic: URI-X, X-KOLEST, ALCO-LIV, and 40 PLUS TONIC. Restriction: Avoid seafood, hot and spicy food, fried food, sour food, pickled food, dairy

products, alcoholic drinks, carbonated drinks, Chinese tea and mineral supplements.

32. HAY FEVER

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Description: An allergy to pollen released by grasses, flowers and trees. The pollen causes cells to

release histamine and other chemicals, resulting in blocked sinuses, a permanently

runny, itchy nose, often a sore, irritated throat, watery eyes and constant sneezing. Cause: Wind-heat invasion in the lungs Treatment: To open the lung qi to expel the wind heat and to restore lung functions. Products to SPITALCARE, BREATH-EZY and P 21 (apply as inhaler inside

Relieve: nose) Restriction: Avoid cold drinks, Chinese tea, carbonated drinks, and dairy products.

33. HEADACHE Description: Pain in the head caused by dilation of cerebral arteries or muscle contractions or a

reaction to drugs or due to continual anxiety, stress and worries. Cause: Excessive stagnation of energy in the liver and gallbladder which then invades the

stomach. Treatment: To strengthen and to tone the stomach and to reduce the tension in the liver; moving

the qi from the liver and gallbladder. Products to

Relieve: ALCO-LIV and X-GAS-3 Restriction: Avoid fried food, hot and spicy food, chilly, dairy products, and drink plenty of

water.

34. HEARTBURN Description: Heartburn caused by indigestion. Cause: Spleen deficiency and liver stagnation. Treatment: Improve function the spleen and treat liver stagnation. Products to

Relieve: SPITALCARE, X-GAS-3, and ALCO-LIV Restriction: Avoid fried, fatty foods, acidic food and "cold" food like cold fruit juice and fruits

from the refrigerator.

35. HEART DISORDERS Description: The condition whereby the force to move blood in the heart is weakened causing

blood and qi stagnation at the same time triggering pain at the point of blockage. Cause: A yang qi deficiency Treatment: 1. Micro Holistic: To warm the heart, tone the energy, clear the blockage and move

the blood. 2. Macro Holistic: To warm the heart, tone the energy, clear the blockage and move

the blood. Palpitations are due to heart yin and blood deficiency and are caused by

stress, fatigue, anxiety and nervousness. The treatment aims to nourish the heart,

lungs and blood. Products to 1. Micro Holistic: X-KOLEST Relieve: 2. Macro Holistic: X-KOLEST, ALCO-LIV, BREATH-EZY and

NERBONCARE Restriction: Avoid high cholesterol foods, fried foods, Chinese tea, dairy products, starchy foods,

alcoholic drinks, vigorous exercise, and late night sleep.

36. HEPATITIS Description: It is a breakdown of liver functions which may in its advance stage may lead to

serious liver damage such as cirrhosis (scarring of the liver), liver failure and liver

cancer, and eventually death. Cause: Liver stagnation caused by external pathogenic influences like hepatitis virus.

Excessive damp heat from the liver and the gallbladder will cause jaundice. Treatment: Dispel liver stagnation Products to

Relieve: VIRA-LIV

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Restriction: Avoid seafood, alcoholic drinks, fried foods, oily foods, and practice safe sex.

37. HIGH BLOOD PRESSURE Description: High blood pressure is diagnosed as internal wind. Low blood pressure is diagnosed

as deficiency of the qi, the blood and the heart. Cause: Weakness in the liver yin, irregularity in the blood and blockage in the blood arteries. Treatment: To detox and nourish the blood, to remove plaque in the arteries, to restore liver

functions, to restore spleen functions and to nourish the kidney yin.

Products to X-KOLEST, ALCO-LIV, STRO-CARE AND 40 PLUS TONIC

Relieve: (NERBONECARE AND SPITALCARE: OPTIONAL)

Restriction: Avoid alcoholic drinks, smoking, table salt, pickled foods, preserved foods, salted

foodstuff, Chinese tea, and fatty foods.

38. HIATUS HERNIA Description: The stomach protrudes up through the diaphragm due to a weakening of the tissue

around the opening of the diaphragm and thus pressing on the valve between it and

the esophagus. Pressure prevents the valve from functioning properly, allowing acid

from the stomach to well up into the esophagus, causing heartburn and sometimes

pain in the neck and arms Cause: Weakening of the tissue around the opening of the diaphragm. Treatment: To nourish and restore the functions of the stomach and also to restore the position of

the diaphragm. Products to

Relieve: SPITALCARE and X-GAS-3 Restriction: Avoid Chinese tea, and fatty foods.

39. IMPOTENCE Description: Impotence is described as the quality of lacking strength or power, being weak and

feeble to achieve penis erection for copulation. Cause: May be due to either physical, psychological, neurological, chemical factors or a side

effect of drug therapy. In body health it is caused by a dysfunction of the lymphatic

(spleen and kidney) and blood circulatory (liver and heart) system. Treatment: 1. Micro Holistic: To nourish and tonify the kidney and liver functions.

2. Macro Holistic: To restore lymphatic and blood circulatory functions Products to 1. Micro Holistic: ADAM’S PRIDE or ERECKING Relieve: 2. Macro Holistic: ADAM’S PRIDE or ERECKING and SPITALCARE,

X-KOLEST, ALCO-LIV and 40 PLUS TONIC Restriction: Avoid Chinese tea and alcoholic drinks.

40. INSOMNIA Description: Insomnia is a sleep disorder that is characterized difficulty in falling and/or staying

asleep. Insomnia is considered to be connected with the heart, kidneys, liver, lungs

and spleen. Insomnia is considered to be connected with the heart, kidneys, liver and

stomach, but particularly with the heart, which houses the shen, or spirit. The TCM

view is that the spirit cannot rest, either because there is heat in the heart, or as a

result of weakness in the kidneys caused by fire and water being out of balance. Cause: Insomnia is considered to be connected with the kidneys, liver, lungs and spleen

disharmony. The heart is the organ of fire, while the kidney is the water organ. Water

normally subdues fire, but if the kidneys are weak there is not enough water to do this

and the fire can get out of control. Over-eating and indigestion caused by a weak

stomach also lead to insomnia. Treatment: To harmonize the functions of the above organs Products to

Relieve: 5-ELEMENTS Restriction: Avoid Chinese tea, deep fried food and cold and sour food.

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41. IRRITABLE BOWEL SYNDROME Description: This is due to a weakness of the muscle action in the walls of the gut, which pushes

down through the intestines into the colon and rectum. It causes spasmodic pain with

explosive, watery diarrhea, alternating with bouts of constipation. Cause: Possibly psychological reasons, and a poor diet with insufficient fiber. TCM regards

it as an imbalance between stomach, spleen and intestines, sometimes also affecting

the liver and kidneys. Normally it is a weakness of the spleen and kidneys, with too

much dampness in the intestines, plus liver qi stagnation. Treatment: To tone the spleen, clear the toxins from the body; ease the bloated feeling in the

stomach, stop constipation, control diarrhea and balance the immune system. Products to

Relieve: SPITALCARE, X-GAS-3 and RENER-LIV. Restriction: Avoid starchy foods, fried foods, dairy products, hot and spicy food, pickled foods,

canned foods and eat plenty vegetables

42. LIGAMENT SPRAIN Description: An injury regarded as a problem of internal blood and qi stagnation. Cause: Sudden exertion of strain against muscles, tendons and ligaments. Treatment: To help move the blood and the qi. Products to 1. Micro Holistic: D T J (should recover within 8 days) Relieve: 2. Macro Holistic: D T J, NERBONECARE and X-KOLEST (for very serious

injury and for areas that it is difficult to apply D T J REGULARLY) Restriction: Avoid soaking affected in cold water.

43. LUMBAGO Description: Backache affecting the lumbar region or lower back. Cause: Muscle strain or arthritis or vascular insufficiency or a ruptured inter-vertebral disc

and weakness in the kidneys to execute functions. Treatment: To restore and tonify kidneys, improve the blood circulation, warm the body and stop

the pain. Products to

Relieve: STEVE-NEK, X-KOLEST and 40 PLUS TONIC Restriction: Avoid massaging affected area if it is not muscle strain as it might be a ruptured inter-

vertebral disc. Avoid hot and spicy food, sour foodstuff, pickled foods, Chinese tea,

and vigorous exercise.

44. MENSTRUAL PROBLEMS Description: In general, these are diagnosed as an imbalance of liver, spleen and kidney. Excessive

blood loss is ascribed to heat in the blood. Late, scanty and painful periods are due to

cold in the blood. Cause: An imbalance interactivity of liver, spleen and kidney; also the liver function to

produce blood every 28 days is affected due to the imbalance of the mentioned

organs. Treatment: To nourish the liver, spleen and kidney and to restore the interactivity of these organs. Products to 1) For painful and heavy periods: Relieve: a) Micro Holistic: EVE’S PRIDE,

b) Macro Holistic: EVE’S PRIDE SPITALCARE and 40 PLUS TONIC.

2) For painful and menstrual irregularities: a) Micro Holistic: EVE’S PRIDE, b) Macro Holistic: EVE’S PRIDE, SPITALCARE, X-KOLEST and

RENER-LIV

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Restriction: Avoid hot and spicy food for heavy periods and to avoid cold food and cold drinks

for irregular and painful periods. Others to avoid are Chinese tea, barley water,

chrysanthemum water, “cooling effect” fruits, and dairy products.

45. MIGRAINE Description: The headaches may be preceded by flashing lights, blurred vision and other visual

disturbances such as an "aura" caused by temporary narrowing of the blood vessels to

the brain. Cause: Excessive stagnation of energy in the liver and gallbladder which invades the

stomach. The pain above the eyes, or flashing lights with pain in the forehead or sides

of the head, indicate involvement of the channels from the liver. The nausea is caused

by liver invasion of the stomach. Treatment: To strengthen and tone the stomach, reduce the tension in the liver, and move the qi

from the liver and gallbladder. Products to

Relieve: X-KOLEST, ALCO-LIV, SPITALCARE and X-GAS-3 Restriction: Avoid fried foods, hot and spicy foods, dairy products, chilly, Chinese tea, tea dust

beverage, and drink plenty of water.

46. MOUTH ULCERS Description: These small round or oval ulcers with a yellowish edge can appear for no apparent

reason, and recur regularly. Cause: Either heat in the heart, heat in the stomach or weakness of the kidneys. If they occur

on the tongue, the cause is heat in the heart, or sometimes the spleen. Ulcers around

the gums are caused by heat in the stomach. If they are all over the mouth, and

constantly recur, this is caused by the kidneys. The spleen and liver hyperactivity also

causes heat accumulation in the spleen, liver and stomach to trigger mouth ulcers. Treatment: To remove heat from the heart, stomach, spleen, liver and to nourish the kidneys. Products to 1) To soothe: - Apply P 21 on affected parts Relieve: 2) To nourish: - RENER-LIV and SPITALCARE (if mouth ulcer is frequent) Restriction: Avoid spicy foods, deep-fried foods or acidic fruit like citrus fruit. Plenty of greens

should be included in the diet, the bowels should be kept open, and plenty of sleep is

also recommended.

47. MULTIPLE SCLEROSIS Description: A chronic progressive nervous disorder involving loss of myelin sheath around

certain nerve fibers. Cause: A general weakness of the whole body, especially the kidneys and spleen which

cause the central nervous system to malfunction and results in wastage of the

muscles. Treatment: To improve the blood circulation, stimulate the nervous system and help to restore

muscles tissues. Revitalize the kidneys and the stomach to stop deterioration. Products to 1) To move blood: X-KOLEST (or X-KOLEST TONIC) and Relieve: NERBONECARE

2) To restore nerves and muscles tissues: STRO-CARE 3) To nourish and tonify kidneys: 40 PLUS TONIC

Restriction: Avoid spicy foods, deep-fried foods or acidic fruit like citrus fruit. Plenty of greens

should be included in the diet, make sure regular bowel movement and plenty of sleep

is also recommended. Avoid dairy products, carbonated drinks, pickled foods, salted

foodstuff, canned foods and seafood.

48. NEPHRITIS Description: Inflammation of the kidneys, often following a streptococcal infection of the throat,

flu or tonsillitis. It usually affects children. The Kidneys cease to function, leading to

raised blood pressure, vomiting or sometimes fits. The urine is scanty, and has a

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smoky or bloody tinge. Fluid retention causes tissue swelling around the eyes, and

repeated attacks can lead to chronic kidney failure. Cause: The problem is connected with the lungs, spleen and kidneys, and all three needs

treatment. There is a blockage of the lungs, causing oedema. Acute nephritis often

follows, and Chinese doctors regard it as the heat from those conditions invading the

lungs. Oedema can also be connected with a weakness of the spleen, tiredness and

swollen limbs, but some kinds also involve kidney deficiency Treatment: To strengthen the lungs, spleen and kidneys. Products to

Relieve: BREATH-EZY, SPITALCARE and 40 PLUS TONIC Restriction: Avoid cold drinks, carbonated drinks, dairy products, hot and spicy foods, fried

foods, sour foods, pickled foods, salted foodstuff and bath after sunset.

49. NEURALGIA Description: Acute nerve pains in the head or face Cause: Wind, fire and heat in the channels connected with the liver and gallbladder. Treatment: To clear wind and internal heat in the liver and gallbladder. Products to

Relieve: ALCO-LIV Restriction: Avoid oily foods, fried foods, curry and chilly, dairy products and alcoholic drinks.

50. NOSE AND THROAT ALIMENTS Description: Frequent throat problems leading to regular coughing episode Cause: Due lungs and spleen deficiency Treatment: Strengthen lungs and spleen. Products to

Relieve: BREATH-EZY and SPITALCARE. Restriction: Avoid cold drinks, carbonated drinks, cooling beverages, cool citrus fruits, sour

foods, pickled foods

51. OEDEMA Description: The body cannot get rid of excess water. The spleen processes the food, the blood and

transforms the dampness in both. The lung is responsible for water retention if there

is a blockage. If the kidney is deficient it will also retain water. Cause: The spleen, lungs and the kidneys are weak and imbalance. Treatment: To nourish and to restore the functions of the spleen, lungs and kidneys. Products to

Relieve: SPITALCARE, BREATH-EZY and 40 PLUS TONIC Restriction: Avoid cold drinks, carbonated drinks, cooling beverages, citrus fruits, milk, dairy

products, sour foods, pickled foods and coffee.

52. OLD AGE JOINTS Description: Weak and at time slight sore joints. Cause: It is the result of wear and tear of joints and due to aging kidneys. Treatment: 1. Micro Holistic: Nourishes the tendons, ligaments, nerves and bones

2. Macro Holistic: Warm the channels of the body and the blood, improving blood

circulation and nourishing the joints. Products to 1. Micro Holistic: NERBONECARE Relieve: 2. Macro Holistic: NERBONECARE, X-KOLEST and RENER-LIV

(or 40 PLUS TONIC) Restriction: It is important to keep warm. Avoid cold drinks, cooling beverages, citrus fruits,

milk, dairy products, sour foods, pickled foods and Chinese tea.

53. OSTEOARTHRITIS

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Description: The result of wear and tear on the joints damaging the cartilage between the bones

due to depleting kidney essence. The joints may become swollen and deformed,

mainly at the hips, knuckles and spine. Cause: Due to weakness in the kidney and the liver, plus a lack of energy causing stagnation

in the blood. Treatment: 1. Micro Holistic: Nourishes the tendons, ligaments, nerves and bones

2. Macro Holistic: Warm the channels of the body and the blood, improving blood circulation and nourishing the joints.

Products to 1. Micro Holistic: NERBONECARE Relieve: 2. Macro Holistic: NERBONECARE, 40 PLUS TONIC, ALCO-LIV

and X-KOLEST Restriction: It is important to keep warm. Avoid cold drinks, cooling beverages, citrus fruits,

milk, dairy products, sour foods, pickled foods and Chinese tea.

54. OSTEOPOROSIS Description: The bones become decalcified due to the loss of calcium which then become

progressively weaker, thinner and more brittle, and can break very easily, particularly

at the pelvis or the wrist. Cause: The kidneys dominate the bones, so osteoporosis is the result of a kidney deficiency.

Note: As the kidneys lie deep in the body, they are not easy to tone quickly, so

treatment must be long-term. Treatment: To tonify the kidneys and to nourish the kidneys. Products to Slow: RENER-LIV Relieve: Fast: 40 PLUS TONIC and RENER-LIV Restriction: Avoid Chinese tea and coffee.

55. OVULATION PROBLEMS Description: Abdominal pain during the menstrual cycle and infertility. Cause: Imbalance of the liver functions and blood production by the liver owing to kidney

deficiencies to balance blood, qi and providing essence to nourish the blood. Treatment: To tonify the liver, nourish blood components, and to nourish the kidneys to provide

essence for blood production. Products to

Relieve: X-KOLEST, ALCO-LIV and 40 PLUS TONIC Restriction: Avoid fried foods, cold drinks, alcoholic drinks, pickled foods, hot and spicy foods

and Chinese tea.

56. PALPITATIONS Description: A symptom of a heart disorder or an overactive thyroid or nervous tension due to

excessive drinking of coffee or a blockage of the heart valves. Cause: A deficiency of the heart and heart blood due to plaques in the blood vessels and

thickening of the blood. Treatment: To remove plaque in blood vessels, thin the blood, restore liver and spleen functions

and strengthen kidneys. Products to X-KOLEST, NERBONECARE, ALCO-LIV, SPITALCARE and Relieve: 40 PLUS TONIC Restriction: Avoid fried and oily foods, dairy products, seafood, sour foods, salted foods, pickled

foodstuff, coffee, alcoholic drinks, carbonated drinks, smoking, and late night sleep.

57. PEPTIC ULCER Description: An ulcer of the mucous membrane lining of the alimentary tract. Cause: Weakness of the spleen, stagnation of stomach qi and stagnation of the liver which

overstrains the stomach. Liver stagnation also causes depression and anxiety, so there

can be an emotional aspect. Treatment: To clear the heat, move the stagnation and help the digestion

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Products to

Relieve: X-KOLEST, SPITALCARE, X-GAS-3 and ALCO-LIV Restriction: Avoid tea, coffee and alcohol particularly when the stomach is empty. Sufferers need

to relax and watch their diet

58. POOR APPETITE Description: Appetite is the desire to eat. Decrease appetite is when our desire to eat reduces. But

poor appetite is the consistent lack of desire to eat. Cause: In TCM, the spleen promotes digestion because it rules transformation and

transportation. It is the primary organ for digestion, absorption and distribution of nutrient essences to the entire body. In order to work properly, the spleen

depends on the warming and propelling activities of kidney-yang. Treatment: To tone up the spleen and nourish the kidneys Products to

Relieve: INFALIVE and RENER-LIV Restriction: Avoid Chinese tea and sour food

59. PROLAPSE Description: The slipping or falling out of place of an organ (e.g. the uterus). Cause: The prolapse of organs is caused by a deficiency of the qi in the middle of the body

that is controlled by the spleen. Treatment: To tone the spleen. Products to

Relieve: SPITALCARE (has to be taken long-term) Restriction: Avoid coffee, Chinese tea and fried foods

60. PSORIASIS Description: A chronic skin disease characterized by dry red patches covered with scales; occurs

especially on the scalp and ears and genitalia and the skin over bony prominences. Cause: Due to a blood heat syndrome. Treatment: To detox the blood and restoring clean blood components. Products to 1) Restoration: SPITALCARE, ALCO-LIV and RENER-LIV Relieve: 2) Application: P 21 Restriction: Avoid fried foods, grilled foods, coffee, Chinese tea, alcoholic drinks, dairy products,

pickled foods and peanuts.

61. RESTLESSNESS Description: Inability to rest or relax or be still. Cause: Due to heat in the heart caused by blood or yin deficiency. Some cases are due to

internal heat. Treatment: To nourish the blood and the yin. Products to

Relieve: X-KOLEST, SPITALCARE and 40 PLUS TONIC Restriction: Avoid fried foods, hot and spicy foods, dairy products, Chinese tea, pickled foods,

salted foodstuff and nuts.

62. RHEUMATOID ARTHRITIS Description: A chronic autoimmune disease with inflammation of the joints and marked

deformities; something (possibly a virus) triggers an attack on the synovium by the

immune system, which releases cytokines that stimulate an inflammatory reaction

that can lead to the destruction of all components of the joint. Cause: General weaknesses of the four vital organs; kidneys, liver, spleen and lungs to

regulate filtration and nourishment functions causing too high acidity in the lymphatic

system. Treatment: To restore the functions of the kidneys, liver, spleen and lungs.

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Products to 1) Detox: URI-X Relieve: 2) Nourishment: 40 PLUS TONIC, ALCO-LIV, SPITALCARE and

BREATH-EZY. Restriction: Avoid total seafood, hot and spicy food, sour foods, pickled foodstuff, coffee,

Chinese tea, cold drinks, carbonated drinks and alcoholic drinks. 63. SAGGING BOSOM Description: When the female mammary glands lose their muscle tone and elasticity and cause

them to droop. Cause: Dysfunction of the spleen due to deficient spleen qi Treatment: To improve function of the spleen, warm it up and dispel dampness. Products to

Relieve: SPITALCARE Restriction: Avoid Chinese tea, cold drinks and fruits

64. SCHIZOPHRENIA Description: This is a serious mental disorder which usually strikes between the teens and

twenties, often affecting very bright and promising young people. They may suffer

delusions of persecution, for example that messages are coming through the

television, or that their minds are being controlled by some hidden force. They may

hear voices instructing them to violent or bizarre acts, and suffer from irrational fears. Cause: A problem of imbalance between heart, kidneys and liver. Treatment: To nourish the heart, kidneys and the liver and then tonify the interactivity between

the organs. Products to

Relieve: X-KOLEST, 40 PLUS TONIC and ALCO-LIV Restriction: Avoid hot and spicy food, salted and pickled foodstuff, dairy products, coffee,

Chinese tea, and alcoholic drinks.

65. SCIATICA Description: Begins when the sciatica nerve is irritated or compressed at its root at the base of the

spine, causing severe shooting pains which will run down the back and outside the leg

from the thigh to the foot. Cause: Damp heat stagnation in the gallbladder meridian or in the urinary bladder meridian. Treatment: To remove heat in the liver and the kidneys and nourishing these organs

simultaneously. Products to

Relieve: X-KOLEST, ALCO-LIV and RENER-LIV Restriction: Avoid fried foods, hot and spicy foods, cold drinks, citrus fruits, coffee, Chinese tea

and carbonated drinks.

66. SINUS PROBLEMS Description: Symptoms are a blocked-up nose, a nasal tone in the speech, and sometimes a

headache over one or both eyes, usually worse in the mornings, when lying down or

bending forwards. Normally mucus is channeled through the tiny air ducts to the

nose, but during colds the ducts can become congested and when the mucus builds

up, the sinuses become inflamed. Cause: Lung qi deficiency, blood stagnation and with damp heat. Treatment: To heat the lungs and to restore the functions. Products to

Relieve: BREATH-EZY, ALCO-LIV and SPITALCARE Restriction: Avoid cold drinks, citrus fruits, coffee, Chinese tea, carbonated drinks, sour foods and

pickled foodstuff.

67. SPERM DISORDERS Description: Low sperm count or having abnormal shaped sperm.

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Cause: A deficiency of kidney essence and yin/yang or exposure to toxins, radiation, PCBs,

solvents, pesticides, heavy metals and pollution or wearing tight fitting clothing and

underwear causing an increase in temperature of the testicle region. Frequent hot

baths in tubs and sauna is also another cause of sperm disorders. Treatment: To remove toxins from the kidneys. To strengthen and tone-up the kidneys. Products to

Relieve: ADAM’S PRIDE or ERECKING and 40 PLUS TONIC Restriction: Avoid wearing tight pants and underwear, soaking in hot water, sauna, coffee,

Chinese tea, pickled foodstuff, sour food and hard liquor.

68. STROKE Description: TCM defines two kinds of stroke; of the head and of the heart. A head stroke is called

a wind stroke and can occasionally be fatal or leave victims partly paralyzed. It

happens when the blood supply to the brain is disturbed or insufficient and can vary

in severity and symptoms. There may be sudden loss of movement or speech,

dizziness, blurred vision, sudden heaviness in the limbs or feelings of numbness, and

a loss of consciousness. If symptoms last for more than twenty-four hours, a full

stroke has occurred. Sometimes they wear off after a few hours, in which case the

condition is called a transient ischemic attack. One in three strokes is fatal and,

depending on the extent of damage, survivors may be left with impairment of speech,

movement or ability to read. Cause: Blood stagnation in blood vessels, plaque in blood vessels, accumulation of wind in

blood vessels due to blood stasis, and blood clots in the brain tissues. Treatment: Detox the liver, kidneys, and the alimentary channel. Nourish the liver and the

kidneys, move the blood and open the channel to clear the wind. Products to 1) Clear the channels: NERBONECARE. Relieve: 2) Move the blood: X-KOLEST and ALCO-LIV

3) Nourish: STRO-CARE Restriction: Avoid fried food, seafood, pickled foodstuff, hot and spicy food, sour foodstuff, dairy

products, alcoholic drinks, carbonated drinks, cold drinks, yellow noodles, and starch

food.

69. SWEATING (EXCESSIVE) Description: The process of the sweat glands of the skin secreting in excess a salty fluid or

tasteless sweat. Cause: Weak lungs and a deficiency of the qi, and in particular the "defensive" qi. Night

sweats are due to yin deficiency. Treatment: To strengthen the lung and to make it warm so that it can tone the qi and also to

restore yin. Products to

Relieve: BREATH-EZY and SPITALCARE Restriction: Avoid cold drinks, iced carbonated drinks, citrus fruits, coffee, Chinese tea and dairy

food.

70. TEETH GRINDING Description: Usually happens during asleep when heat rises from the spleen and the stomach; may

also be the presence of some kind of parasite in the intestines. Cause: Heat in the spleen and the stomach; also the presence of parasites in the intestines. Treatment: To cool the heat in the spleen and to detox the digestive system. Products to

Relieve: Restore: SPITALCARE Restriction: Avoid hot and spicy food, fried food, chilly, coffee, Chinese tea and dairy products.

71. TENDON DISORDERS

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Description: Tendons weakened due to attacks by diseases (e.g. chronic arthritis) and mainly due

to poor blood circulation. Cause: Weak liver and spleen. Treatment: To nourish the liver, the spleen, and to improve blood circulation by nourishing blood

and removing plaque in blood vessels. Products to

Relieve: ALCO-LIV, SPITALCARE, X-KOLEST and NERBONECARE Restriction: Avoid fried foods, grilled foods, oily foodstuff, seafood, pickled food, sour food,

chocolate, coffee and Chinese tea.

72. TENNIS ELBOW Description: Inflammation of the tendon which joins the muscles of the forearm to the lower end

of the upper arm bone (the humerus). The muscles at the back of the forearm flex the

fingers and wrists, and they are all connected to the same point on the outer side of

the humerus. Not surprisingly, in view of the muscles' constant use, inflammation can

sometimes result, causing extreme tenderness and pain down the forearm and up to

the shoulder. Any repetition action - typing, working at a supermarket checking out,

or repeated lifting - can cause this condition, as well as playing tennis frequently. Cause: Cold and damp in the elbow region as a result of poor blood circulation. Treatment: To improve the quality of blood and to remove blockage in blood vessels so that the

tendons are well nourished. Products to X-KOLEST, NERBONECARE and RENER-LIV. (Add D T J for

Relieve: soothing) Restriction: Avoid seafood, cold drinks, alcoholic drinks, coffee and Chinese tea.

73. TIC Description: A nervous twitch of a muscle in the face, this is also known as habit spasm. Cause: Wind in the liver caused by the accumulation of heat in the liver or by a liver blood

deficiency. Treatment: To remove toxins in the liver, nourish the liver and nourish the blood. Products to

Relieve: ALCO-LIV, SPITALCARE and X-KOLEST Restriction: Avoid fried foods, dairy products, salted foodstuff, pickled foodstuff, curry, coffee

and Chinese tea.

74. TINNITUS Description: This condition, which causes ringing in the ears, can stem from the ear itself, but may

also be caused by high blood pressure and other problems. Cause: Chronic weakness of kidneys and poor blood circulation. Treatment: To restore essence in the kidneys and to increase blood circulation. Products to

Relieve: 40 PLUS TONIC, X-KOLEST and NERBONECARE Restriction: Avoid citrus fruits, cold drinks, carbonated drinks, sour food, pickled foodstuff,

coffee and Chinese tea.

75. TONSILLITIS Description: Inflammation of the tonsils leading to a very sore throat at the back of the throat. The

neck glands are also affected in many cases, and there may be a dry cough. Cause: Wind and heat or, sometimes, to fire (burn) poison within the liver, spleen, stomach,

and lungs. Treatment: To remove toxins in the liver, spleen, stomach and lungs and to restore their

interactive metabolisms. Products to

Relieve: 40 PLUS TONIC and BREATH-EZY

Page 144

Restriction: Avoid spicy food, fried food, chilly, curry, dairy products, liquor, cold drinks and

citrus fruits.

76. UTERINE DISORDERS Description: These include anything from cancer or fibroids to certain kinds of colic or simply

infections. Cause: Accumulation of phlegm and blood stasis in the uterine. Treatment: To dislodge and to prevent phlegm accumulation and to remove blood stasis. Products to

Relieve: EVE’S PRIDE, SPITALCARE, BREATH-EZY and 40 PLUS TONIC Restriction: Avoid cold drinks, sour food, pickled foodstuff, hot and spicy food, carbonated

drinks, pickled food, dairy products, starchy food and sweetened foodstuff

77. VARICOSE VEINS Description: These are veins that have become enlarged and twisted Cause: Stagnation of the blood and qi leading to fermenting heat and damp which erupt into

ulcer. Treatment: The damp heat has to be cleared and the circulation of the blood and qi improved. Products to

Relieve: X-KOLEST and NERBONECARE. Restriction: Avoid fried foods, grilled food, pickled foodstuff, sour food, seafood, salted

foodstuff, dairy products, coffee, Chinese tea, icy food and starchy cakes.

78. VERTIGO Description: The condition is often triggered by fear of falling from a height, or during bouts of

nausea and vomiting. Sufferers feel that their head is spinning when in fact it is quite

still. Cause: Inflammation of the balance organ in the inner ear, or by some other ear disorder.

Three possible causes: a deficiency of the blood and the qi (common in old people or

after serious illness), or liver wind or phlegm in the body. Treatment: To improve the quality of blood, blood circulation, restore liver functions and prevent

phlegm accumulation in the lungs. Products to

Relieve: X-KOLEST, ALCO-LIV, NERBONECARE and BREATH-EZY. Restriction: Avoid fried food, dairy products, coffee, Chinese tea, oily food, salted foodstuff,

and pickled foodstuff

79. WEAK LEGS Description: It is a condition whereby the legs are unable to walk long distances and climb slopes

or stairs. Untreated they could lead to permanent disability and permanently wheel

chair bound. Cause: In TCM, kidneys rule the bones. Kidney yin (essence) is needed for healthy bones.

The spleen rules the muscles, flesh and the four limbs. It does this by transporting the

body's energy and blood to the muscles, flesh, arms and legs. Treatment: Restore kidneys and spleen deficiency Products to

Relieve: RUN ON Restriction: Avoid Chinese tea, cold drinks and fruits, and sour food

80. WEAK LUNGS Description: When a person suffers from frequent cold and flu. Cause: Deficient lung qi Treatment: Nourish the lungs Products to

Relieve: BREATH-EZY

Page 145

Restriction: Avoid Chinese tea, cold drinks and fruits, and sour food

81. WEIGHT PROBLEMS Description: The common perception about people who are overweight is that they become that

way through overeating, but this is not necessarily the case. Some people eat less than

others, yet still put on weight. There are two points of influence here - what you eat

and what you are. Some people have a more efficient metabolism than others, and

process the food more efficiently. Too many fatty foods and dairy products are not good for the system, particularly in

someone with a tendency to gain weight. Rice and vegetables is a much more sensible

diet. It may not be necessary to eat less, just to change your eating habits. Cause: Weakness of the kidneys, liver (liver stagnation) and spleen. Sluggish metabolism

produces a lot of internal phlegm and dampness. Treatment: To remove toxins in the liver, kidneys, stomach, large intestines and small intestines,

and to restore kidney, liver and spleen functions. Products to 1) Detox: COLONEASE Relieve: 2) Lipolysis: SLYM-CARE

3) Restore: 40 PLUS TONIC, ALCO-LIV and SPITALCARE Restriction: Avoid fried food, starchy food, oily food, dairy products, carbonated drinks, yellow

noodles (mee), fatty food and any fast food.

Page 146

Buku Panduan

Produk

Kenyataan

Buku Panduan Produk in adalah hak cipta pemilik Awal Mart Corporation Sdn. Bhd dan

dikeluarkan secara khusus untuk kegunaan pengedar/ahli kami yang sah dan atau sekutu

perniagaan yang berkaitan. Ia mangandungi perincian tentang huraian produk-produk

berkaitan dengan senarai produk-produk yang terkandung di antaranya, penyediaan Herba

TCM Awal Mart Group dan atau produk makanan yang mana berkenaan adalah diluluskan

oleh Kementarian Kesihatan Malaysia. Buku Panduan produk in dihasilkan untuk tujuan

maklumat sahaja dan tanpa asas prejudis. Ia tidak bermaksud untuk menyumbang juga

pergantungan keatas keperluan mana-mana professional atau nasihat ahli perubatan yang

berkelayakan dalam apa-apa pun pada bahagian kami

Tiada bahagian dalam penerbitan ini yang boleh dicetak ulang dalam bentuk apa sekalipun

atau diedarkan kepada orang lain tanpa keizinnan bertulis daripada pihak kami.

Hakcipta Nutri-Huang Sdn. Bhd. 2009. Hakcipta adalah

terpelihara.

AwalMart_Catalogue2

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