A S C A S AWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002 AWACSS AWACSS Dr. Dale M. Willard, Universität Tübingen (Gauglitz Group) Contract #: EVK1-CT-2000-00045 Duration: 3 Year, RTD Project (Starting March, 2001) Visit: http://barolo.ipc.uni-tuebingen.de/awacss A S C A S Automated Water Analyser Computer Supported System
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AWACSS (EVK1-CT-2000-00045)ENVSENS, Lecce, Italy, 2002 AWACSS Dr. Dale M. Willard, Universität Tübingen (Gauglitz Group) Contract #: EVK1-CT-2000-00045.
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Goal: Develop a cost-effective, on-line, water monitoring Goal: Develop a cost-effective, on-line, water monitoring biosensor that will measure a variety of small organic pollutants biosensor that will measure a variety of small organic pollutants
in short-time with remote control and surveillance.in short-time with remote control and surveillance.
Expanded multi-analyte analysis capability allowing for up to 32 simultaneous analyte measurements. Immunochemical reagents are being developed for a host of small organic water pollutants.
Novel design approaches to the optical detection and fluidics including miniturized integrated optics and microfluidics. Reduced reagent volumes. Limits of detection matching EU prescribed requirements (~1 ppb).
Intelligent remote surveillance and control that will allow for unattended continuous monitoring. Instruments will be rugged and easy to handle by untrained personnel.
Methodology: Immunoassay coupled with Total Internal Methodology: Immunoassay coupled with Total Internal Reflection FluorescenceReflection Fluorescence
Laser
IO-ChipWaveguide
Detection
PC
SamplesWaste
SignalCollection
Fluidics
Analyte derivative
Analyte
Antibody
Fluorophor
Analyte derivative
Analyte
Antibody
Fluorophor
Analyte
Antibody
Fluorophore
* *
Flow
Transducer
* *
Flow
Remaining free antibodies arecaptured and detected.
* *
Flow
* *
FlowSamples are pre-incubatedwith labelled antibodies.
Progress:• Measurement station software modules are selected and requirements are defined.• The first generation software simulator is complete.• The network design is defined.
Goals:• Cataloguing and monitoring local water quality using standard methods (HPLC). Data helps community and project establish trends and identify problems.• Establishing monitoring sites for final instruments.• Defining requirements of end-users, validating design issues, and testing final instruments.
Water Monitoring ProgressWater Monitoring Progress
• Cross-referencing exercise was performed and standardised protocols for sampling, storing, shipment, and extraction developed.
• Target sites for field deployment: Saint Joan Despi (Llobregat River, Spain), Worms (Rhine River, Germany), Novaky Chemical Plant (Nitra River, Slovak Republic).
• Data Collection: 12 measurement sites, 36 compounds9 compounds recorded significant levels
PartnersPartnersEberhard-Karls-University Tübingen: Project Management and Surface Immunoassay Development
Central Research Laboratory: Instrument Hardward Design
Siemens: Electronics, Internet Communication, and Market Analysis.
Optoelectronics Research Centre: Integrated Optical Chips
Environmental Institute, Kos: Instrument Design and Field Tests-Slovakia
King‘s College London: Immunochemistry
Institute of Chem. & Environ. Chem. IIQAB: Instrument Design and Field Tests-Spain
DVGW-Technologiezentrum Wasser: Instrument Design and Field Tests-Germany
Funding: This Project is funded under the 5th Framework Programme: Energy, Environment, andSustainable Development. Visit: http://barolo.ipc.uni-tuebingen.de/awacss.