Medicines from Nature: Adventures of a medical ethnobotanist Cassandra L. Quave, Ph.D. Assistant Professor Emory University Department of Dermatology (SOM) Center for the Study of Human Health (ECAS) E-mail: [email protected]Website: http://etnobotanica.us/
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Atlanta Botanical Garden Science Cafe: Medicines from Nature - 2014
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Medicines from Nature: Adventures of a medical ethnobotanist
Elmleaf Blackberry • Traditional uses in S. Italy:
▫ Leaves: furuncles, abscesses,
and other skin inflammations
▫ Roots: hair loss
▫ Fruits: eaten fresh and in
marmalades
• One of 116 remedies related to
SSTIs and other topical
dermatological treatments
identified
• 168 extracts screened
• Anti-biofilm activity first identified & published in 2008 and # 220 marked as possible lead
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Rubus ulmifolius Schott. (Rosaceae):
The source of the bioactive
composition “220D-F2”.
Quave, C.L., A. Pieroni, and B.C. Bennett (2008) Dermatological remedies in the traditional pharmacopoeia of Vulture-Alto Bradano,
inland southern Italy. Journal of Ethnobiology and Ethnomedicine 4:5. Quave, C.L., L.R.W. Plano, *T. Pantuso, and B.C. Bennett (2008). Effects of extracts from Italian medicinal plants on planktonic growth,
biofilm formation and adherence in MRSA. Journal of Ethnopharmacology 118: 418-428
Following bioassay-guided fractionation steps, ellagitannins and
glycosylated ellagic acid derivatives were isolated from the extract.
Activity of blackberry root extract is based on several natural products
Quave, C.L., M.E. Carmona, C.M. Compadre, *G. Hobby, H. Hendrickson, K. Beenken, and M.S. Smeltzer. (2012). Ellagic acid
derivatives from Rubus ulmifolius inhibit Staphylococcus aureus biofilm formation and improve response to antibiotics. PLoS ONE. 7(1): e28737.
220D-F2 is effective against all clonal lineages of S.
aureus, regardless of antibiotic resistance profile and is
nontoxic to mammalian cell lines.
Blackberry root extract prevents Staphylococcus aureus biofilms from forming
Quave, C.L., M.E. Carmona, C.M. Compadre, *G. Hobby, H. Hendrickson, K. Beenken, and M.S. Smeltzer. (2012). Ellagic acid
derivatives from Rubus ulmifolius inhibit Staphylococcus aureus biofilm formation and improve response to antibiotics. PLoS ONE. 7(1): e28737.
220D-F2 improves response to functionally distinct classes of antibiotics,
including daptomycin, clindamycin, vancomycin, and oxacillin.
Blackberry root extract improves antibiotic efficacy in clearing Staphylococcus
aureus biofilms
Quave, C.L., M.E. Carmona, C.M. Compadre, *G. Hobby, H. Hendrickson, K. Beenken, and M.S. Smeltzer. (2012). Ellagic acid
derivatives from Rubus ulmifolius inhibit Staphylococcus aureus biofilm formation and improve response to antibiotics. PLoS ONE. 7(1): e28737.
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C
Killing of planktonic pneumococci by 220D-F2. Streptococcus pneumoniae strain D39 was
inoculated in 24 well-plates containing THY and treated with DMSO or the indicated concentration of 220D-F2; treated cultures were incubated for 3 h at 37°C. Planktonic cells were removed (A) and then biofilms were washed and removed (B). Both populations were diluted and plated onto BAP to obtain CFU/ml. (C) Planktonic pneumococci treated for 3 h were also stained by the LIVE/DEAD assay and imaged using a fluorescent microscope.
Talekar, S.J., S. Chochua, K. Nelson, K.P. Klugman, C.L. Quave and J.E. Vidal (2014). 220D-F2 from Rubus ulmifolius kills Streptococcus pneumoniae planktonic cells and pneumococcal biofilms. PLoS ONE 9(5): e97314.
Blackberry root extract destroys Streptococcus pneumoniae biofilms and kills cells
NE Albania, 2012 Wild foods and medicinal
plants used by Albanians and
the Gorani
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Mountain Landscapes – NE Albania
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Kukes District, Albania
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Local economy
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Fermented Foods
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Quave, C.L. and A. Pieroni (2014) Fermented foods for food sovereignty and food security in the Balkans: A case study of the Gorani people of northeastern Albania. Journal of Ethnobiology 34(1): 28-43.