Atache Lift Therapy Scientific Presentation

LIFT THERAPY PROFESSIONAL PROGRAM

(5 sessions)

LIFT EXCELLENCEConcentrated facial firming

solution

SCULPT LIFT SHOCKTensor effect final emulsion

MODEL LIFT MASKFirming face and neck mask

PERFORMANCE NECK SOLUTION PROFESSIONALFirming serum for double chin and neck

PERFORMANCE NECK SOLUTION PROFESSIONAL

Firming serum for double chin and neck

PACKAGING : single-dose tube 4mlCOSMETIC FORM: cream -gel. DESCRIPTION:Effective firming serum especially for neck and double chinIt combines firming, tensor and slimming active ingredients.BENEFITS:It improves firmness, elasticity and hydration.It strengthens and restructures thin skin.


Firming Serum for double chin and neck

• LIPOBELLE SOYAGLICONE, 3%• DSH CN, silanol derivative of high

molecular weight hyaluronic acid, 5%• RENOVHYAL Low molecular weight

hyaluronic acid, 0.5%• ESSENSKIN, 2.5%• SLIMBUSTER, 3%

ACTIVE INGREDIENTS

IT PROMOTES ABSORPTION OF ACTIVE INGREDIENTS:

ARLASOLVE DMI-PC 3%


Firming serum for double chin and neck• ESSENSKIN

An additional source of calcium and essential amino acids through the synergisticassociation of Calcium -α-Hydroxymethionine and Homotaurine.

It fortifies and restructures thin and fragile skin



ACTIVE INGREDIENT: ESSENSKIN

Young skin

Mature skin

No calcium gradient

Calcium gradient

In young people, calcium is very present in the upper part of the granular layer, but nearly absent in the spinous layer. This calcium gradient alters with age. Cell renewal becomes slower. The epidermis becomes thin and dry.


• ESSENSKINCa- HYDROXYMETHIONINE

Calcium:In skin, the calcium gradient regulates homeostasis of the epidermal barrier and differentiation.

Methionine: an essential amino acid, containing sulphur.

It acts as a mediator in the biosynthesis of amino acids and phospholipids.

It is a key element in the process of protein synthesis, inactivates free radicals and participates in DNA repair, protecting and preserving cells.


• ESSENSKIN

Taurine: the role of taurine in the maintenance of ion pumps and calcium homeostasis, as well as in neuroprotection, has been clearly described.

With age, taurine levels fall off. Homotaurine is an analogue more stable than taurine. It regulates the ionic balance, affecting chlorine, calcium, sodium and potassium channels through GABA receptors. The homotaurine contributes to the maintenance of the skin barrier homeostasis.



IN VIVO• Skin thickness• Firmness and elasticity• Roughness• Radiant skin

• ESSENSKIN

Efficacy tests:

IN VITRO

• Gene activation tests

• Cell differentiation

• Cell proliferation

• Osmotic protection and flow management



• ESSENSKIN

Essenskin™ improves the epidermal homeostasis and restores the main cellular functions:� Differentiation� Proliferation� Protein and lipid synthesis� Skin recovers its thickness, firmness, elasticity and smoothness.


Firming serum for double chin and neck• IN VIVO:

+ 65%Skin thickness

Visible improvement of thickness in both epidermal and dermal layers with EssenskinTM



Variation %

Placebo Variation %Significance versus Placebo

Average variation (%) for the 23 respondantsSignificance versus Placebo

Elasticity Firmness

T1 month T2 months T1 month T2 months

An increase in firmness and elasticity is observed in 80% of the panelists after 1 and 2 months.

Firmness

RoughnessAfter 2 monthsThickness of peaks by mm2...........................................-22.3%Distance between peaks (µm).......................................+30.1%Significance versus placebo p=0.01 and p<0.1, respectively

An improvement of the “crêpe paper” appearance of the skin. Skin is smoother and has a younger appearance .

ReflectanceReflectance is the amount of light reflected (specular reflectance + diffuse reflectance). It depends on the opacity of the epidermis and it increases with age

Specular reflectance

Diffuse reflectance

Total reflectance

RESULTS AFTER TWO MONTHS

Densified skin....................................+ 65%Thicker skin....................................... .......+21%



• SLIMBUSTER:

Coffea Arabica (Coffee) Seed Oil and sterified phytosterols from Brassica campestris

It is an agent with effective skin properties that stimulates elasticity and firmness through increasing the synthesis of essential fibre.

Four benefits:1-It leads to lipolysis and inhibits lipogenesis in the adipose tissue.

2-It stimulates the synthesis of tissue growth factor, and induces tissue repair.3-It induces tissue repair.

4-It develops the synthesis of the extracellular matrix compounds (ECM), mainly collagen I and II, elastin and glycosaminoglycans.

SLIMBUSTER

1- SLIMMING EFFECT

Conclusions:

• It promotes intense lipolysis, by reducing the amount of triglycerides within cells.

• Reduction of fat.

CULTURE OF HUMAN ADIPOCYTES TREATED WITH 2.5%



• Lipobelle SoyagliconeLiposomal preparation of genistein (biologically active form of themost abundant soy isoflavone). Liposomal genistein is able to penetrate deeply into the stratum corneum.


Firming Serum for double chain and neck

Lipobelle Soyaglicone:

�Biologically active genistein.

�Genistein is an effective collagen stimulator.

Transformation of glycosylated genistein (genistein) without biological activity into

active genistein.



• Lipobelle Soyaglicone: Action Mechanism, mild estrogenic effect and inhibition of tyrosine kinase.



• Lipobelle Soyaglicone:In vivo efficacy study.

• Re-densifying effect. Efficacy of a cream containing 3% of Lipobelle Soyaglicone.

• A 12% increase of skin thickness after 3 months.



• It stimulates the synthesis of collagen IV. Efficacy of a cream containing a 3% of Lipobelle Soyaglicone.

• The immunofluorescence staining of collagen IV and the cell nucleus, showed a 53% increase of collagen IV.

�Lipobelle Soyaglicone : In vivo efficacy study.


Firming Serum for double chin and neckLipobelle Soyaglicone : In vivo efficacy study

Lipobelle Soyaglicone

�Provides a visible lifting effect.

�Reduces lines and wrinkles.

�Re-densifies and firms the skin.

�Skin appears 5 years younger.



DSH CN AND RENOVHYAL: HYALURONIC ACID

A great number of these molecules

provides a structure with a high hydration,

responsible for the skin turgor .

Macromolecule derived from the linear polymerisation of two glucose derivatives: the glucuronic acid and the N-acetylglucosamine.


Hyaluronic acid is naturally present in all human tissue.This molecule is similar in all species and in all types of tissue.

Its function is to firmly contribute to the maintenance of water and to the structure of the skin.


� Main component of the skin.

� This component decreases with age and because of external aggressions.

� The addition of hyaluronic acid through different means, allows for an excellent hydration and elasticity of the skin, reducing wrinkles.

Hyaluronic acid holds water, hydrating and giving volume as well as smoothing the skin.


�Depending on its number of units, hyaluronic acid has a certain molecular weight.

�The highest molecular weightacts on a superficial level, it has a high hydrating and non-occlusive filling power. It provides long-term hydration.

�The lowest molecular weightpenetrates the skin.



DSH CN: Dimethyl Silanol Hyaluronate.

� Organic silicon with hyaluronate. It is an organic derivative rich in high molecular weight hyaluronic acid.

� It has an effective hydrating action, both long and short term.

� Cytostimulating effect on fibroblasts.



RENOVHYAL: Sodium Hyaluronate (biotechnological origin)

Low molecular weight hyaluronic acid

-Keeps skin hydrated by reinforcing the intercellular bonds responsible for cellular cohesion.- Increases firmness of the skin, stimulating the synthesis of collagen I.

RENOVHYAL: Sodium HyaluronateMechanism of action

HYDRATION:

It stimulates the synthesis of binding proteins.

It prevents transepidermal water loss.

WATER RETENTION IN THE DERMIS

FIRMNESS:

It stimulates the synthesis of collagen I

STIMULANTS OF THE ABSORPTION OF ACTIVE INGREDIENTS

• ARLASOLVE DMI-PC 3%



STIMULANTS OF THE ABSORPTION OF ACTIVE INGREDIENTS

ARLASOLVE DMI-PC: DIMETHYL ISOSORBIDE

High-purity ingredient, which is being developed to enhance absorption of active ingredients:

� It enhances formulation and stability.

� It increases absorption through the epidermis.

� It reduces cell inflammation.

LIFT EXCELLENCEConcentrated facial firming solution

PACKAGING : single-dose tube 4 mlCOSMETIC FORM: cream -gel. DESCRIPTION:Effective firming serum.It combines firming and tensor active ingredients.BENEFITS:It provides firmness, elasticity and hydration.


ACTIVE INGREDIENTS:� Gatuline In-Tense, 2%� Lipobelle Soyaglicone, 3%� DSH CN, 5%, silanol derivative of high

molecular weight hyaluronic acid.� Renovhyal, 0.5%

Low molecular weight hyaluronic acid.


• Gatuline In- Tense

Spilanthes acmella oily extract.Its main components are alkylamides , ensuring a minimum of 0.5%. New concentrated firming and anti-wrinkle active ingredients.It stimulates the natural skin firming properties .


Gatuline In- Tense

Collagen fibres

Fibroblasts

Elastin fibres

With age, cell dynamics and the interaction between cells and support fibres decrease. This results in a disorganization of the collagen structure.

Gatuline® In-Tense can reorganise and recover the structure of collagen fibres.


Gatuline In- Tense• In vitro efficacy• It has a tensor and firming effect. It has

been tested on live artificial dermis.

• The effect has been evaluated by measuring the surface of contraction, using cytokine tGFβ as positive control. Kinetics of tissue contraction after 7 days.


Gatuline In-Tense• In vitro efficacy

Efficacy was evaluated after 7 days. Visible results from the very first day. Power of tissue contraction on day 1.


Clinical study:• 28 women aged 45 to 65, applied the product twice

daily for 28 days.

Gatuline In-Tense

Before and after pictures show:The smoothing action of Gatuline® In-Tense. A visible reduction of wrinkled skin.


Gatuline In-TenseClinical study. Visible results


Gatuline In-TenseClinical study: Ultra-rapid action – results on Day 1

(profilometry)

Anti-wrinkle effect on Day 1.Differences between placebo and Gatuline® In-Tense


Gatuline In-TenseClinical study Visible results

Anti-wrinkle effect on day 1

Wrinkles may be reduced up to 51% from the first application.

Gatuline In-Tense

�Firming active ingredient, which is extremely rapid and efficient.

� Its action on the support tissue allows a rapid reorganization of the collagen fibres, as well as a visible smoothing of the skin.

Gatuline In- Tense

� It reduces wrinkles, as well as the most visible signs of ageing, which is the wrinkled appearance of skin.


ACTIVE INGREDIENTS:�Gatuline In-Tense, 2%�Lipobelle Soyaglicone, 3%�DSH CN, 5%. Silanol derivative of high

molecular weight hyaluronic acid.� Renovhyal, 0.5%. Low molecular

weight hyaluronic acid.

MODEL LIFT MASK

Firming face and neck

Alginate Peel-off Mask with:

Firming action: since it is rich in silicon, it provides firmness and prevents flaccidity.

Occlusive action: it increases the temperature and helps the absorption of active ingredients.Silicon content of 6%


PACKAGING : tube 30 MLCOSMETIC FORM: Emulsion. DESCRIPTION:Final product in the treatment, only for 5 sessions of the professional treatment.It combines firming and tensor active ingredients.BENEFITS:It provides firmness, elasticity, hydration and a tensor effect.

ACTIVE INGREDIENTS:�Gatuline In-Tense, 2%

�Lipobelle Soyaglicone, 3%

�DSH CN, 6%

�Nab Siberian Ginseng 5%



Nab Siberian Ginseng:Eleutherococcus senticosus.Ingredient rich in sugar, which shows its effect by leaving a thin layer on the skin when it dries.It is important to warn clients not to touch their skin for a few minutes after its application.

PRODUCTS FOR SALE

• LIFT THERAPY FOR SALE

� PERFORMANCE NECK SOLUTION :Firming solution for double chin and neck

� INTENSIVE LIFT CONTOUR :Emulsion for eye and lip liner

� FORCE LIFT DAY:Firming day facial emulsion

� SUBLIME LIFT NIGHT : Night firming serum

PERFORMANCE NECK SOLUTION Firming solution for double chin and neck

PACKAGING : cream jar 50 ML.COSMETIC FORM : cream-gel.DESCRIPTION:- Effective firming cream especially for neck and double chin.- It combines firming, tensor and slimming active ingredients.BENEFITS:- It provides firmness, elasticity and hydration.- It strengthens and restructures thin skin.

�Lipobelle Soyaglicone, 3%�DSH CN, silanol derivative of high

molecular weight hyaluronic acid, 5%�RENOVHYAL, low molecular weight

hyaluronic acid 0.5%�Essenskin, 2.5%�Slimbuster, 3%

PERFORMANCE NECK SOLUTIONFirming solution for double chin and neck

ACTIVE INGREDIENTS:

INTENSIVE LIFT CONTOUR :Eye and lip outliner

PACKAGING : TUBE 15 ML.COSMETIC FORM : Emulsion. DESCRIPTION:- Firming cream specific for eye and lip outliner.- It combines firming, tensor and anti-expression lines active ingredients.BENEFITS:- Firmness, elasticity, and hydration.- It reduces expression lines.

pH 6.0-7.0

INTENSIVE LIFT CONTOUR : Eye and lip outliner

ACTIVE INGREDIENTS:�Gatuline IN- TENSE, 2%

�DSH CN,silanol derivative of high molecular weight hyaluronic acid, 3%

�SNAP-8, 10%

�SYNTHETIC TRIPEPTIDE, based on the snake’s venom ( SYN-AKE), 2%

INTENSIVE LIFT CONTOUR : Eye and lip outliner

• SNAP-8Anti-expression line octapeptide: Acetyl Octapeptide-3

� Octapeptide (combination of 8 amino acids)

� Evolution of argireline with an improved efficacy

� Trial IN VIVO of the anti-wrinkle activity:

Maximum reduction 63.23%

Average reduction 34.98%

PRESYNAPTIC NEURON

Transport of Ach

Acetylcholine Ach

Synaptic vesicle

Acetylcholine released (Ach)

SYNAPTIC SPACE

Temple viper, the inspiration

Source: McArdle, JJ, T L Lentz, V Witzemann, H Schwarz, SA Weinstein & JJ Schmidt. 1999. Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor. J. Pharmacol. Exp. Therap. 289:543-550.

Viper venom contains proteins with different functions:• Digestion• Paralysis• ToxinsWaglerin 1 is an antagonist of the muscle nicotinic acetylcholine receptor (mnAChR).

Waglerin 1 blocks the neuromuscular contraction.



• SYN-AKE

A synthetic tripeptide that acts against expression lines when face muscles are relaxed. It was developed when investigating viper venom, and it acts in a similar way to Waglerin 1 , a neuromuscular blocker found in the venom of the Temple Viper.

NH

NH

O

O

NH2

N

ONH2

Patent WO 2006/047900 (11.5.06)

• Synthetic tripeptide with a molecular weight lower than 500 Da for optimal skin absorption.

• It mimics a small sequence of Waglerin 1 found in the venom of the Temple Viper.

• INCI (active): Dipeptide Diaminobutyroyl Benzylamide Diacetate.


Signal transduction in the synaptic fissure.

SYN®-AKE reduces the transduction of the neuronal signa l by binding to mnAChR.


Frequency of contraction depending on the post-incubation time (Concentration of peptide : 0.5 mM)

• SYN®-AKE reduces the contraction of muscle cells.• Fast , long-lasting and totally reversible action.


IN VITRO EFFICACY : Contraction of the innervated muscle cel ls

• Double-blind intra-individual study • Measurements are taken before/after• 15 volunteers per group (women, aged 41-60)• Was applied on the face twice daily for 28 days• Assessment of the anti-wrinkle effect with

Primos 3D®

• Crow’s feet and forehead

In Vivo EfficacyStudy design

INTENSIVE LIFT CONTOUR : Eye and lip liner

In Vivo EfficacyCrow’s feet wrinkles

Measurement of smoothing (Ra) and anti-wrinkle effect (Rz and Rt) of SYN®-AKE (4%) in crow’s feet after 28 days.VOLUNTEER #49 Day 0

Day 28

Effect of SYN®-AKE (4%) in smoothing (Ra) and anti-wrinkle effect (Rz and Rt) measured in the forehead after 28 days , compared with a competitor’s product (10%) and placebo.

-2%-4%

-21%

-2%

-20%

-1%-3%

-15%

0%

-25%

-15%

-5%

PlaceboAcetyl hexapeptide-3

solution (10%) SYN-AKE (4%)

Ra Rz Rt

** (**) p≤0.01(*) p<0.05

Wrin

kle

para

met

er [%

of d

ay 0

]

In vivo EfficacyWrinkles in the forehead

**

*

In Vivo EfficacyWrinkles in the forehead

Measurement of the anti-wrinkle effect in volunteer #9 (mesured in the forehead after 28 days)

Day 0Day 28

Day 28

Day 0

FORCE LIFT DAY : Firming day facial emulsion

PACKAGING: Tube 50 MLCOSMETIC FORM : Emulsion DESCRIPTION:Firming DAY emulsion, specific for the face. It combines firming, tensor and antioxidant active ingredients.BENEFITS:It provides firmness, elasticity, hydration and sun protection.

FORCE LIFT DAY:Firming day facial emulsion

� GATULINE IN-TENSE, 2%

� LIPOBELLE SOYAGLYCONE, 3%

�VITAMIN E ACETATE, 0.5%

� SUNSCREEN, (FPS 20)

SUBLIME LIFT NIGHT :Night firming serum

PACKAGING : Dropper bottle 30 ML.COSMETIC FORM: Serum (water/glycerine)DESCRIPTION:NIGHT firming serum specific for the face. It combines firming, tensor and anti-expression line active ingredients.BENEFIT:It provides firmness, elasticity, and hydration.It reduces expression lines.

SUBLIME LIFT NIGHT :

Night firming serum

� GATULINE IN-TENSE, 2%� LIPOBELLE SOYAGLYCONE, 3%� SNAP-8, 3% � SYNTHETIC TRIPEPTIDE, based on snake venom ( SYN-AKE), 2%� DSH CN, 6%� RENOVHYAL 0.5%� ARLASOLVE DMI-PC, 3%

ACTIVE INGREDIENTS:

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