Aseptic technique
Dec 11, 2015
You will need a flame to light the bunsen burner
• If you are using a torch, do NOT keep striking it, if there is no spark – your fellow students would like to continue to breathe
• You will NEVER get a bunsen burner to light if there is no spark in the torch
Your right pinky is now the most important part to perform the next steps
• Hold the tube with the bacterial culture with your left hand
• Remove the cap with your pinky from your right hand
• In the aseptic technique, you want to try NOT to put anything down on the counter if you do not have to
Every time you open or close your bacterial culture, you want to flame your tube
• Remember, the cap is being held by your pinky of your right hand and is NOT on the counter
• With the tube in your left hand, pass it through the flame
• If you need to use the loop, hold it between your thumb and forefinger of your right hand
Time to clean up• When you are done with the cultures, place them in the
rack or container provided by your instructor
• Make sure that you have turned off the flame
• All bacterial plates that you do not want must be placed in the orange bags
• Tubes that you do not want should be placed in the RACK in the back of the room – they are not discarded
• Wipe down your bench with O-SYL