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Intranasal immunization with synthetic peptides corresponding to the E6and E7 oncoproteins of human papillomavirus type 16 induces systemic
and mucosal cellular immune responses and tumor protection
Pallavi R. Manuri a, Bharti Nehete b, Pramod N. Nehete b, Rose Reisenauer a,Seth Wardell a, Amy N. Courtney a, Ratish Gambhira b, Dakshyani Lomada c,
Ashok K. Chopra d, K. Jagannadha Sastry a,b,∗a Department of Immunology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, United States
b Department of Veterinary Sciences, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, United Statesc Department of Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, United States
d Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555, United States
Received 22 June 2006; received in revised form 20 December 2006; accepted 4 January 2007
bstract
The E6 and E7 oncoproteins of the high-risk HPV type16 represent ideal targets for HPV vaccine development, they being consistentlyxpressed in cervical cancer lesions. Since HPV-16 is primarily transmitted through genital mucosal route, mucosal immune responsesonstitute an essential feature for vaccination strategies against HPV-associated lesions. We present here evidence showing that mucosalmmunization of mice by the intranasal route with a mixture of peptides E744–62 and E643–57 from the E7 and E6 oncoproteins of HPV-16,espectively, using a mutant cholera toxin adjuvant (CT-2*), primed strong antigen-specific cellular immune responses in systemic and mucosalissues. Significant levels of IFN-� production by both CD4 and CD8 cells were observed along with CTL responses that were effective againstoth peptide-pulsed targets as well as syngeneic tumor cells (TC-1) expressing the cognate E6 and E7 proteins. Furthermore, mice immunized
*
Cith the peptide mixture and CT-2 effectively resisted TC-1 tumor challenge. These results together with our earlier observations that T cellesponses to these peptides correlate with recurrence-free survival in women after ablative treatment for HPV-associated cervical intraepithelialeoplasia, support the potential of these E6 and E7 peptides for inclusion in vaccine formulations.
2007 Published by Elsevier Ltd.
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Eeywords: HPV16 E7 and E6 peptides; Mucosal immunization; Mutant ch
. Introduction
Papillomaviruses are small DNA viruses that infect ver-ebrate hosts, including humans and cause the formation
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f hyperproliferative epithelial lesions [1]. The Oncogenicigh-risk types of human papillomaviruses (HPV) suchs HPV-16 are the main causative factors in the patho-
∗ Corresponding author at: Department of Immunology, UT M.D. Ander-on Cancer Center, 7455 Fannin, Unit 901, Houston, TX 77030, Unitedtates. Tel.: +1 713 563 3304; fax: +1 713 563 3276.
enesis of cervical carcinomas [2]. Several strategies forPV16 vaccines have been developed and evaluated in ani-al models. These included the use of recombinant E7
rotein [3], DNA vaccine encoding E7 [4], and bacte-ial/viral vectors expressing E7 or E7 epitopes [5–7] as wells CTL epitope peptides of E7 [8]. All of these strate-ies represent parenteral routes of immunization, but HPVs a sexually transmitted mucosal pathogen and, therefore,
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
ucosal vaccination may be necessary for protection. Both 15
he oral and intranasal routes constitute attractive strategies 16
or priming systemic as well as mucosal immunity against 17
Delivery of virus like particles (VLPs), corresponding toPV16 and 18, by the oral route was shown to be effec-
ive in generating antigen-specific immune responses in micend these could be significantly enhanced by the use ofucosal adjuvants like, LT192G, the non-toxic mutant form
f Escherishia coli heat-labile enterotoxin (LT), or GpG-ontaining oligodeoxynucleotides [9]. However, intranasalmmunization has emerged as the optimal vaccination strat-gy in rodents for induction of antibody responses in genitalissues [10–12] and was also shown to be effective in humantudies [11,13,14]. Intranasal immunization of mice withPV16 L1 protein or the HPV16 L1 gene in combinationith the adjuvant cholera toxin was shown to elicit sys-
emic and mucosal humoral and cellular immune responses15]. Recently, it was reported that intranasal immunizationf mice with live lactococci expressing the E7 antigen andL-12 induced systemic and mucosal immune responses andlso protected mice against challenge with an E7-expressingurine tumor cell line TC-1 [16].Vaccines based on peptide antigens have been proposed
nd pursued by several groups for a variety of pathogensecause the advantages related to safety and ease of pro-uction, but their weak immunogenic properties must bevercome through the use of adjuvants, fusion proteins, ornchor-modified peptide epitopes. The adjuvant propertiesf cholera toxin (CT), an enterotoxin produced by Vibrioholerae have been described extensively [17,18]. Sincehe native CT is unsuitable for human use because of itsoxic effects, many mutants lacking toxicity but retainingheir adjuvanticity have been developed and characterized19,20]. The mucosal adjuvanticity of CT-2*, a choleraoxin mutant derived by introducing two-codon substitutionsArg7-Lys and Glu112-Gln) into the CT-A subunit [21] waseported earlier [22] where intranasal (i.n.) administrationf a CTL epitope peptide from HIV-1 with CT-2* gener-ted strong mucosal and systemic immune responses. Here,e obtained data demonstrating that i.n. administration of a
ombination of HPV-16 E744–62 and E643–57 peptides alongith CT-2* as the adjuvant induced antigen-specific cellular
mmune responses at various mucosal and systemic com-artments and protected mice against challenge with TC-1umor cells expressing the E6 and E7 oncoproteins of HPV-6.
. Materials and methods
.1. Animals
Female C57BL/6 mice of 6–8 weeks age were purchasedrom NCI. All the procedures for handling the animals werearried out in accordance with institutionally approved pro-
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ocols. The animals were housed in microisolator cages androvided with sterile food and water. The animal facilitys fully accredited by the Association for Assessment andccreditation of Laboratory Animals Care International. The
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tudies were conducted according to National Institute ofealth Guidelines on the care and use of Laboratory Animals.
.2. Cell lines and cell cultures
The cell lines EL-4 (C57BL/6, H-2b, Thymoma) andAC-1 were maintained in RPMI complete media (CM)upplemented with 10% heat-inactivated FBS, 50 U/ml ofenicillin–streptomycin and 50 �g/ml gentamycin. The TC-tumor cells are primary lung epithelial cells of C57BL/6ice origin that were transfected to express the E6 and E7
ncogenes of HPV-16 as described earlier [23] and werekind gift from Dr. T.-C. Wu, Johns Hopkins Medical
nstitution, Baltimore, MD. The TC-1 cells were grownn complete RPMI supplemented with 400 �g/ml of G418.he EG7·Ova cells were EL-4 thymoma cells transfectedith chicken ovalbumin [24] and were a kind gift from Dr.hen, Dept. of Immunology, UTMD Anderson Cancer Cen-
er. They were maintained in DMEM supplemented with 10%eat-inactivated FBS, 2 mM glutamine, 100 U/ml penicillin,00 �g streptomycin and 400 �g/ml G418.
.3. Peptides
The E744–62 peptide, Q19D (QAEPDRAHVYNIVTFC-KCD) and the E643–57 peptide, Q15L (QLLRREVYD-AFRDL) were used for immunization of mice. In additiono these peptides, the E749–57 peptide, R9F (RAHVYNIVTF)nd the E649–58 peptide, V10C (VYDFAFRDLC) that repre-ent murine H2b-restricted CTL epitopes, were used in theTL assays. All the peptides were prepared in the insti-
utional antigen-core facility utilizing FMOC solid phasehemistry on a PTI Symphony Peptide Synthesizer (Pro-ein Technologies Inc., Tucson, Arizona). Peptide purity wasetermined to be >95% by high-pressure liquid chromatog-aphy (HPLC) and was validated by mass spectrometry.
.4. Mutant cholera toxin (CT-2*)
The CT-2* protein, a mutant form of the cholera toxin,roduced by a vaccine strain of V. cholerae, was purifiedo homogeneity by sodium hexametaphosphate precipitation,ffinity purification on a galactose column and Sephadex G75el filtration chromatography as described earlier [25–28].he purified toxin was dissolved in pyrogen-free water,nd the lipopolysaccharide (LPS) contamination was deter-ined by the Limulus amebocyte lysate assay (QCL-1000kit,ioWhitaker, Walkerville, MD). The amount of LPS detected
n 1 �g of CT-2* (amount used as adjuvant in mice) was.5 pg, which did not stimulate production of any cytokine inhe mouse ligated ileal loops [25–28].
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
.5. Immunizations 116
Mice (n = 5) were immunized by the intranasal route 117
wice at 5-day intervals with a mixture of the Q19D and 118
15L peptides (100 �g of each/mouse) along with CT-2*
1 �g/mouse). The mice were anaesthetized by the intraperi-oneal injection of ketamine–xylaxine mixture and weremmunized by introducing a small volume (10–15 �l) con-isting of the peptide mixture and the CT-2* adjuvant in PBSnto each nostril. Five days after the last immunization, miceere sacrificed and cell suspensions were prepared from the
pleen, cervical lymph nodes (CLN), mesentric lymph nodesMLN), Peyer’s patches (PP), and the vaginal-associatedymphoid tissue (VALT) by homogenization or enzymaticissociation using collagenase type IV (Sigma).
.6. CTL assay
The CTL assay was carried out as described previously29]. Briefly, cells isolated from different tissues were res-imulated for 5 days with the cognate peptide mixture usedor immunizing the mice. The cytolytic activity of the res-imulated cells was assayed against syngeneic 51Cr-labeledL-4 cells that were incubated with either medium or the
ndividual cognate peptides. Additional targets included the1Cr-labeled TC-1 cells expressing the E6 and E7 oncogenesf HPV-16. Unlabeled Yac-1 cells were mixed with theabeled TC-1 cells to eliminate contribution from the NK cellysis for calculating the antigen-specific CTL activity. Theercentage (%) of specific lysis was calculated using the fol-owing formula: % specific lysis = (experimental release −pontaneous release)/(maximum release-spontaneouselease) × 100, where the spontaneous release represents theadioactivity obtained when the target cells were incubatedn culture medium without effectors and maximum releaseepresents the radioactivity obtained when the target cellsere lysed with 1% Triton X-100.
.7. Intracellular cytokine staining and flow cytometrynalysis
Cells isolated from the various tissues were stimulatedith the individual E6 and E7 peptides used for immu-izing mice, and the percentages of CD4+ and CD8+
cells producing IFN-� were determined by the intra-ellular cytokine flow cytometry [30]. The peptides fortimulating the various cells were used at a concentrationf 2 �g/ml for 4 h at 37 ◦C, with the Golgistop reagent1 �l/ml, Pharmingen, San Diego, CA) added for an addi-ional 8 h before harvesting the cells from the culture.ells were then washed once with FACS buffer (PBS + 1%BS + 0.1% sodium azide) and stained with the followingorochrome-conjugated monoclonal rat anti-mouse antibod-
es (Pharmingen, San Diego, CA): CD3-APC, CD8-FITCnd CD4-PerCP. Cells were then subjected to intracellularytokine staining using the Cytofix/Cytoperm kit accord-
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ng to the manufacturer’s instructions (Pharmingen) andE-conjugated anti-IFN-� antibody or the immunoglobulin
sotype control antibody (rat IgG1), both purchased fromharmingen. Sample acquisition was done on a FACScal-
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bur and analyzed using the Flowjo 6.4.2 software (Bectonickinson, CA).
.8. Measurement of IFN-γ production by ELISPOTssay
Cells isolated from spleen, CLN, and MLN of immunizedice were subjected to ELISPOT assay for antigen specific
FN-�-producing cells as described earlier [22] using the kitrom Pharmingen, San Diego, CA. The spots, representingndividual IFN-�-producing cell as spot forming cells (SFC),n the membrane were enumerated by Zellnet Consultingnc., New York, NY using the KS-ELISPOT automatic sys-em (Carl Zeisis Inc., Thornwood, NY). Responses wereonsidered positive when they were above 50 SFC/well andt least double the number obtained in cells cultured withedium alone. A non-specific negative peptide was included
s another control. Peptide specific and negative controlesponses were compared with the responses of medium and-values ≤0.05 (*) were considered significant.
.9. In vivo tumor protection experiments
Two groups of mice (n = 5) were immunized by the i.n.oute twice at 5 days intervals with the mixture of the HPV-6 peptides E744–62 and E643–57 (100 �g each) along with theutant cholera toxin, CT-2* (1 �g). Five days after the last
mmunization, one group of mice were injected in the rightank by the subcutaneous route with 2 × 105 TC-1 tumorells, and the other group of mice that served as a negativeontrol group, were injected with 5 × 106 EG7·Ova tumorells. A separate group of naı̈ve unvaccinated mice (n = 5)njected with the TC-1 tumor cells (2 × 105) served as anotheregative control group. Mice in all the groups were monitoredwice a week for tumor growth, and the tumor size was mea-ured using a caliper and was recorded as mean diameter:ongest surface length (a) and width (b), and the tumor sizealculated using the formula, (a + b)/2. Mice were euthanizedhen the tumor size reached 20 mm in mean diameter.
. Results
.1. Intranasal immunization with a combination of the7 peptide Q19D and the E6 peptide Q15L of HPV-16long with the mutant cholera toxin CT-2* adjuvantrimes antigen-specific CTL that lyse syngeneic TC-1umor cells expressing the cognate proteins
A group of five C57BL/6 mice were intranasally immu-ized twice at 5-day intervals with a mixture of HPV-16eptides Q19D and Q15L (E744–62 and E643–57, respectively)
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
dmixed with CT-2*, a two-codon mutant of cholera toxin, 215
bserved in our earlier studies to be effective as a mucosal 216
djuvant [22]. Analyses of cells from the spleens and drain- 217
ng cervical lymph nodes (CLN) showed strong CTL activity 218
gainst target cells pulsed with either Q19D or Q15L peptidesFig. 1). The specific lysis values obtained at an effector toarget cell (E:T) ratio of 100:1 were 75% in spleen and 50% inLN for Q19D peptide and 58% in spleen and 40% in CLN
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or Q15L peptide. These responses were found to be highlyignificant when compared to EL-4 targets (p ≤ 0.00005).hese effectors also lysed EL-4 target cells pulsed with pep-
ides R9F (E749–57) and V10C (E649–58) (p ≤ 0.05), the CTL
ig. 1. Intranasal immunization with the mixture of E744–62 (Q19D) and643–57 (Q15L) peptides from HPV-16 using the mutant cholera toxin (CT-*) adjuvant induces CTL responses. C57Bl/6 mice were immunized by thentranasal route with peptides Q19D and Q15L (100 �g each) and CT-2*
1 �g) a total of two times at 5 days intervals. Cells isolated from the spleennd cervical lymph nodes (CLN) were restimulated for 5 days in vitro with19D and Q15L peptides before assaying for CTL activity. (A) The res-
imulated spleen cells (panel A) and CLN (panel B) were tested for CTLctivity against MHC-matched EL-4 target cells pulsed with Q19D, R9F,15L or V10C peptides or TC-1 tumor cells. The 51Cr-labelled TC-1 cellsere mixed with unlabelled YAC-1 cells that serve as targets for NK activ-
ty, to determine the tumor-cell-specific lysis. The EG7·Ova tumor targetsxpressing Ova protein served as negative control target cells for ascertaininghe antigen specific lysis of the TC-1 cells. The horizontal line in each panelndicates the cut-off value for positive response (10% antigen-specific lysis).ata shown is an average of at least three separate experiments. Student’s
-test analysis was done by comparing with the EL-4 empty targets to deter-ine the p-values. Values ≤0.00005 have been assigned (***), p ≤ 0.0005
**), and p ≤ 0.005 (*).
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pitopes within the Q19D and Q15L sequences, respectively.urthermore, significant lysis of the TC-1 tumor cells thatxpress the E7 and E6 oncoproteins of HPV-16 was alsobserved by the spleen and CLN cells from these immu-ized mice. The specific lysis values at an E:T ratio of 100:1ere 38% and 20% for the spleen and the CLN cells, respec-
ively (Fig. 1). The values were compared to those obtainedith EG7·Ova targets and were found to be highly signifi-
ant (p ≤ 0.00005). No CTL activity was observed both in thepleen and CLN against either the Yac-1 cells (NK targets) orhe EG7·Ova cells (syngeneic tumor cells expressing the non-pecific ovalbumin antigen) used as targets (Fig. 1) indicatinghe antigen-specificity of the CTL responses induced.
.2. Immunization with the mixture of the Q19D and15L peptides induces IFN-γ production by CD4+ andD8+ T cells from the systemic and mucosalompartments
We also determined whether the mucosal immunizationrotocol adopted was effective in priming antigen-specificellular immune responses in different mucosal and sys-emic compartments. For this, cells isolated from differentissues of mice immunized with the mixture of Q19D and15L peptides along with the CT-2* adjuvant were assayed
or antigen-specific IFN-� production by CD8+ and CD4+
cells. As shown in Fig. 2A we observed significant per-entages of CD8+ T cells producing IFN-� in response totimulation with the Q19D peptide in the spleen (7.88%),he draining cervical lymph nodes (4.58%), as well as mul-iple mucosal tissues like the MLN (6.26%), Peyer’s patches9.41%), and the VALT (14.5%). Similar responses werebserved in the cells from the various tissues in response totimulation with the Q15L peptide (7.92% in spleen, 5.95%n CLN, 5.96% in MLN, 11.7% in PP, and 11.3% in VALT).
e also observed strong IFN-� production by CD4+ T cells inesponse to both Q19D and Q15L (Fig. 2B). No positive CD8nd CD4 responses were observed for all the tissues with theegative control peptide used for stimulation (Fig. 2A and B).verall, the amounts of IFN-� producing CD8+ T cells were
omparable to that of CD4+ T cells in response to Q19D asell as Q15L peptides (Fig. 2C).We also measured the number of antigen-specific IFN-�
roducing cells generated by intranasal immunization withhe HPV peptides and CT-2* on the day of sacrifice byLISPOT assay. Fig. 3A depicts the number of IFN-� spot
orming cells observed in the spleens of mice immunizedith CT-2* and the combination of HPV16 HPV16E744–62
nd E643–57 peptides. The number of spot forming cells in thepleen was significantly higher for the peptides (Q19D and15L) when compared to those in the medium alone con-
rol (p ≤ 0.05). Similar analyses were performed using cells
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
solated from the CLN and MLN. As shown in Figure 3B 277
ositive peptide specific responses were significantly higher 278
han the medium control (p ≤ 0.05) in mice immunized with 279
T-2* and the combination of HPV16 HPV16E744–62 and 280
643–57 peptides. Responses to the negative peptide were notositive according to the criteria described in the methodsection in spleen (Fig. 3A), CLN and MLN (Fig. 3B).
.3. Protection against TC-1 tumor growth by intranasalmmunization with the mixture of the Q19D and Q15Leptides
In order to determine the in vivo efficacy of the cellu-ar immune responses induced after i.n. immunization of
ice with the combination of the Q19D and Q15L pep-ides using the CT-2* adjuvant, we adopted a tumor-challenge
odel. The TC-1 cells are syngeneic to the C57BL/6 micend express the E6 and E7 oncoproteins of HPV-16 [23].
group of naı̈ve mice injected with the TC-1 tumor cellserved as unvaccinated controls (group 1). At seven days after
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he second i.n. immunization, mice were injected with eitherhe EG7·Ova tumor cells that are syngeneic but express therrelevant ovalbumin antigen (group 2) or the TC-1 tumorells (group 3). Mice in all the three groups were monitored
toua
ig. 2. Intranasal immunization with the mixture of E744–62 (Q19D) and E643–57 (Q1rimes antigen-specific IFN-� production by CD4+ and CD8+ T cells. Cells isolatey the intranasal route with mixture of Q19D and Q15L peptides were stimulatedeptide individually (2 �g/ml) for determining the IFN-� producing in (A) CD8+
arkers and for intracellular IFN-� as described in the methods section. The numercentages of CD8+/IFN-�+ CD4+/IFN-�+ (double-positive) T cells are shown in tf five separate experiments performed. (C) The bar graph shows the average valuedouble-positive) T cells in 1 × 106 cells specific to peptides Q19D and Q15L in sply comparing with medium alone and values ≤0.05 (*) are considered significant.
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or tumor development over 15 days. As shown in Fig. 4A,days after injection of TC-1 tumor cells mice in groupdeveloped tumors with a mean size of 14.15 ± 1.15 mm.
imilarly, mice in group 2 that received the EG7·Ova tumorells, tumors started growing by day 7 and reached a meanize of 11.15 ± 0.36 mm. However, mice in group 3 immu-ized with the HPV peptides (Q19D and Q15L along with theT-2* antigen) and injected with TC-1 tumors had no mea-
urable tumors (p < 0.05 as compared with EG7·Ova groupnd naı̈ve group). By day 15, naı̈ve mice injected with theC-1 tumor cells (group 1) as well as the mice injected with
he EG7·Ova tumor cells (group 2) showed tumors measuringnd 17.0 ± 0.5 mm and 16.15 ± 0.23, respectively (Fig. 4A).n the other hand, mice in group 3 immunized with the HPVeptides and injected with TC-1 tumor cells did not developny measurable tumors by day 15. Analyses of the data for
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ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
he percentages of mice in each group that were free of tumor 315
ver a period of 60 days follow-up showed 15% of the naı̈ve 316
nimmunized mice challenged with TC-1 tumors (group 1) 317
nd 20% of immunized mice challenged with the EG7·Ova 318
5L) peptides from HPV-16 using the mutant cholera toxin (CT-2*) adjuvantd from various mucosal and systemic tissues of C57BL/6 mice immunizedin vitro using medium cognate peptides or a non-specific negative controland (B) CD4+ T cells. Cells were stained for CD3, CD4 and CD8 surfacebers of IFN-�-producing T cells were analyzed by flow cytometry and thehe upper right corner of each dot plot. The data shown is one representatives along with standard errors of percentages of CD8+/IFN-�+ CD4+/IFN-�+
een, CLN, MLN, Peyer’s patches and VALT. The p-values were determined
umors (group 2) were without tumors (Fig. 4B) while nonen the group 3 mice showed tumors. Between days 15–18, allhe mice in the groups 1 and 2 developed large tumors while00% of the mice immunized with the mixture of E7 and6 peptides (Q19D and Q15L, respectively) along with theT-2* as adjuvant and challenged with the TC-1 tumors were
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umor-free throughout the follow-up period of 60 days. Theseesults clearly demonstrate the efficacy of intranasal immu-ization with the mixture of the HPV-16 peptides against theC-1 tumor challenge.
dppb
ued ).
. Discussion
Both the E7 and E6 oncoproteins of HPV are expressed inre-neoplastic as well as cancerous lesions of the cervix andepresent potential targets for prophylaxis and immunother-py approaches. Most studies to date focused on the
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
evelopment of vaccines employing either the whole E7 or E6 333
roteins in separate formulations or individual immunogenic 334
eptides corresponding to these proteins [30–33]. Peptide- 335
ased vaccines have gained much importance over the last 336
Fig. 3. Induction of peptide specific IFN-� producing cells by intranasalimmunization with the mixture of E744–62 (Q19D) and E643–57 (Q15L) pep-tides from HPV-16 using the mutant cholera toxin (CT-2*) as adjuvant. Cellsisolated from spleen, CLN and MLN of C57BL/6 mice immunized by theintranasal route with mixture of Q19D and Q15L peptides were stimulated invitro using medium, cognate peptides or a non-specific negative control pep-tide individually (2 �g/ml) in a 96 well plate ELISPOT. After 48 h the plateswere washed and developed and the number IFN-� producing spot formingcells were enumerated in each well and the number of spot forming cellscalculated per 1 × 106 cells is plotted. Average values and errors bars werecalculated from two separate experiments consisting of three mice in each.Responses were considered positive when they were above 50 SFC/well andat least double the number obtained in cells cultured with medium alone (thiscca
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Fig. 4. Intranasal immunization with the mixture of E744–62 (Q19D) andE643–57 (Q15L) peptides from HPV-16 using the mutant cholera toxin (CT-2*) adjuvant protects mice against challenge with the TC-1 tumor cells. Threeexperimental groups of C57BL/6 mice were included: naı̈ve unimmunizedmice were injected subcutaneously on the right flank with 1 × 105 TC-1tumor cells per mouse (group 1, n = 6); mice immunized intranasally withthe mixture of Q19D and Q15L peptides (100 �g each) along with CT-2*
adjuvant (1 �g) twice at 5 days intervals and 1 week after the last immuniza-tion injected subcutaneously on the right flank with either 1 × 106 EG7·Ovatumor cells per mouse (group 2, n = 6) or 2 × 105 TC-1 tumor cells per mouse(group 3, n = 10). The size of the tumors (length + width/2) on days 7 and15 was measured in all three groups were measured and the average valuesplotted (panel A). Panel B shows the percentages of mice tumor-free over aperiod of 60 days follow-up in the three different groups: Data for group 1ifS
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tides used for immunization of mice were observed in the 363
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Rut-off value shown as horizontal line in the two panels). The p-values werealculated comparing with the medium alone control and values ≤0.05 (*)re considered significant.
ecade as they are relatively easy to construct and pro-uce, chemically stable, and unlike the E6 and E7 proteins,ack oncogenic potential. Many investigators have developedowerful paradigms to choose peptides from the E6 and E7ncoproteins of HPV-16 for immunization and establishedhe pre-clinical evaluation to develop peptide-based tumoraccines [8,34,35].
In a previous study [36], we demonstrated the immuno-enic potential of the Q19D and Q15L peptides from the7 and E6 oncoprotein of HPV-16 (E744–62 and E643–57,
espectively), delivered individually by the parenteral route
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sing the Freund’s complete adjuvant, for inducing antigen-pecific CTL responses in mice. A 35-amino acid E7 peptideverlapping with the Q19D peptide, but also encompassing
Vaw
s shown as light gray line, as dark gray line for group 2, and as black lineor group 3. Statistical analysis between the three groups was done using thetudent’s t-test.
n E7-derived Th epitope, potentiated antigen specific CTLesponses in mice [32]. The Q15L peptide from the E6 onco-rotein used in our study overlaps with a murine CTL epitopeeptide (E648–57, presented by H-2Kb) reported by Pengt al. [37]. Results from the present investigation demon-trated that intranasal immunization with a mixture of the E7nd E6 peptides, Q19D and Q15L, using the mucosal adju-ant CT-2*, induced strong antigen-specific T cell responseshat afforded protection against challenge with tumor cellsxpressing the E6 and E7 oncoproteins of HPV-16. Impor-antly, antigen-specific T cell responses, in terms of IFN-�roducing CD4+ and CD8+ T cells in response to the pep-
ization with synthetic peptides corresponding to the E6 and E7ucosal cellular immune responses and tumor protection, Vaccine
ALT. No positive peptide specific antibody responses (IgG 364
nd IgA) were observed in the serum, saliva and vaginal 365
ashes (data not shown) tested at different time points by 366
he immunization procedure followed (two doses at 5 daysntervals). The significance of this data lies in the fact thatPV is a sexually transmitted pathogen, and the develop-ent of an HPV vaccine/adjuvant formulation capable of
liciting mucosal immune responses, including at the genitalucosal surfaces, may be important for protection againstPV-associated cervical lesions. Furthermore, in a previous
ross-sectional study we observed a significant associationf cellular immune responses specific to these E7 and E6eptides, Q19D and Q15L, with recurrence-free survival ofomen that underwent ablative treatment for HPV-associated
ervical intraepithelial neoplasia.Even though literature reports described DNA vaccines
ased on the E7 protein or the immunodominant CTL epi-opes to be effective in generating HPV-specific systemicmmunity [30], prevalence of antigen specific T cell responsest distant mucosal sites has not been investigated in detail.imilarly, the efficacy of delivering the E7 protein along withpG-oligodeoxynucleotide (ODN), a strong immunomodu-
atory agent [38–41], for priming systemic but not mucosalmmune responses was reported by Tae-Yoon et al. [31].ne recent report described intranasal immunizations with
n adeno-associated virus (AAV2) vector construct express-ng the codon-optimized major capsid gene L1 (L1H) fromPV-16 to induce long-lasting humoral and cellular immune
esponses [42]. In our study, we show for the first time thato-delivery of peptides from the E7 and E6 proteins of HPV-6 along with the non-toxic mucosal adjuvant CT-2* by thentranasal route elicited strong systemic and mucosal cellularmmune responses along with anti-tumor efficacy. We believehat an ideal vaccine for the prophylaxis and therapy of HPV-ssociated cancers should prime strong immunity at multipleucosal sites and exhibit protective efficacy, and results from
he present investigation provide strong support for the util-ty of these E6 and E7 peptides and the intranasal deliverytrategy for potential clinical testing.
cknowledgements
All the culture media were produced by the central mediaab, and all the synthetic peptides were prepared in the Syn-hetic Antigen Core Facility, both supported by funds fromIH grant CA 16672.Conflict of interest statement: None of the authors have a
ommercial association that might pose conflict of interest.Funding: Studies in this manuscript are supported in part
y funds from the NCI grant CA077378, NIAID AI42694,nstitutional grant MRP from M.D. Anderson Cancer Center.
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