Applications of cell lines Presented by: Arshvir kaur M.pharm 1 st year
Applications of cell lines
Presented by:
Arshvir kaur
M.pharm 1st year
Cell culture
Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown
under controlled conditions. But in practice it refers to the culturing of cells derived
from animal cells.
Cell culture was first successfully undertaken by Ross Harrison in 1907
Roux in 1885 for the first time maintained embryonic chick cells in a cell culture.
Why is cell culture used for?
Areas where cell culture technology is currently playing a major role.
Model systems for
Studying basic cell biology, interactions between disease causing agents and cells,
effects of drugs on cells, process and triggering of aging & nutritional studies
Toxicity testing
Study the effects of new drugs
Cancer research
Study the function of various chemicals, virus & radiation to convert normal cultured
cells to cancerous cells
Conti…..
Virology
Cultivation of virus for vaccine production, also used to study there infectious cycle. e.g.
polio, rabies, chicken pox, hepatitis B & measles.
Gene therapy
Cells having a functional gene can be replaced to cells which are having non-functional
gene.
Tissue culture
•In vitro cultivation of organs, tissues & cells at defined temperature using an incubator
& supplemented with a medium containing cell nutrients & growth factors is collectively
known as tissue culture.
•Different types of cell grown in culture includes connective tissue elements such as
fibroblasts, skeletal tissue, cardiac, epithelial tissue (liver, breast, skin, kidney) and many
different types of tumor cells.
Primary culture
•Cells when surgically or enzymatically removed from an organism and placed in suitable
culture environment will attach and grow are called as primary culture.
•Primary cells have a finite life span.
•Primary culture contains a very heterogeneous population of cells.
•Treated by proteolytic enzyme (Trypsin).
Cell lines
A cell line is a product of immortal cells that are used for biological research.
Cells used for cell lines are immortal, that happens if a cell is cancerous.
The cells can perpetuate division indefinitely which is unlike regular cells which can only
divide approximately 50 times.
WRL-68HeLa Jurkat
Common cell lines
Human cell lines
MCF-7 breast cancer
HL 60 Leukemia
HEK-293 Human embryonic kidney
HeLa Henrietta lacks
Primate cell lines
Vero African green monkey kidney epithelial cells
Cos-7 African green monkey kidney cells
And others such as CHO from hamster, sf9 & sf21 from insect cells.
Cell line
Species of origin
Tissue of origin
Cell morphology
Growth in suspension?
3T3 Mouse Connective Fibroblast No
CHO ChineseHamster
Ovary Epithelial Yes
BHK21 SyrianHamster
Kidney Fibroblast Yes
HeLa Human CervicalCarcinoma
Epithelial Yes
Continuous cell lines
Cell lines which either occur spontaneously or induced virally or chemically
transformed into Continuous cell lines
Characteristics of continuous cell lines
• -smaller, more rounded, less adherent with a higher nucleus /cytoplasm
ratio
• -Fast growth and have aneuploid chromosome number
Introduction of Cell Culture Lab(Equipment)
CO2-thermostats
Airflow
Solutions
Dishes
Freezers
Liquid nitrogen
Centrifuges
Vacuum ovens
Cryotubes
Microscopes
ELISA-readers
Autoclave
Co2 incubator Dishes
CentrifugesAutoclaves
Vacuum Ovens
ELISA readers
What is in the media?
Dulbecco’ Modified Eagle’s Media (DMEM)
Contains glucose, some proteins, and essential salts
Contains a pH indicator (phenol red) Media looks pink/red at pH 7.2 Acidic -yellow or orange (cell growth, bacterial growth)
Basic -purple (no cell growth, not enough CO2)
Antibiotics (penicillin and streptomycin) Prevent bacterial contamination
Salts and buffers To simulate in vivo environment
Conti……
SerumPortion of blood after the cells and fibers have clotted
From cow, horse, sheep
added to media as a nutrient source for growing cells
Lipids, proteins
Use of tissue cultures in toxicity testing
Mammalian cell cultures can be a suitable alternative for the use of whole animal tests
to establish the potential toxicity of compounds.
This due to many reasons:
•They can overcome the disadvantages of the whole animal tests including:
High cost
Variability of results
•Cell culture tests are ripid,allow more efficient screening of novel compounds and sometimes can
allow identification of metabolic target of inhibition.
Conti…..
Cell culture tests can be designed to evaluate various effect:
Reduced growth rate
Breakdown of membrane permeability
Tissue specificity response
Ability to metabolize toxic compound
Genetic effect
Use of tissue culture for biological products
Production of vaccines:
• Two factors stimulated the use of tissue cultures for vaccine production:
▫ The ability to grow viruses in cell culture
Current egg vaccine production require long time(9 month) that hinder the response to
unanticipated demands.
In (1949),Enders discovered that the poliomyelitis virus could be grown from primary
monkey cells in culture.
The polio vaccine, produced in 1954, was the first human vaccine to be produced using
large-scale cell culture techniques.
Conti…
Animal cell technology is considerably developed for the production of a range of human and veterinary viral vaccines against a variety of diseases.
(b) Production of antibodies:
Also, the in vitro methods for production of mABs are the methods of
choice because of:
The ease of culture for production.
Less economic consideration compared with the use of animals.
Conti…..
Practical uses of the in vitro produced mABs:
Diagnostic tests for the identification of small quantities of specific antigens.
mABs also are used therapeutically: OKT3 recognizes a surface antigen (CD3) on T cell
and is one of the most effective agents in preventing immunological rejection of
transplanted kidneys.
Conti…..
Various mAbs designed to destruct tumor cells by targeting a membrane bound
protein antigens specifically expressed by these cells.
The conjugation of radiactive or toxic compounds to the antibody can result in a
localized high concentration resulting in cytotoxicity to the target cells.
Conti….
(c)Recombinant proteins:
Proteins extracted from biological sources have been important for the substitution
therapy.
since the 1920s when Best and Banting used insulin to treat diabetes.
Conti….
Some examples for these biological products:
Interferone:
Discovered when Isaacs and Lindenmann (1957) found that culture medium taken from cells that
had supported viral growth could protect non-infected cells from a subsequent viral infection.
Tissue plasminogen activator (t-PA ):
t-PA was produced in large scale by Genenteck from transfected CHO-K1 cells. It is used to prevent
undesirable formation fibrin clots in the bloodstream.
Blood clotting factors:
For example, factor VIII is produced in large scale by Bayer through transfection of the mammalian
kidney cell line (BHK) with an appropriate gene.
Cell therapy
Literally, cell therapy means treatment with cells, i.e replacing diseased or
dysfunctional cells with healthy functioning ones.
For example:
•When hematopoietic cells are vulnerable to destruction by any cytotoxic drugs used in
chemotherapy to eradicate residual tumor cells.
Drug screening & development
• Cell based assay have become increasingly important for the pharmaceutical industry,
not just for cytotoxicity testing but also for high throughput screening of compounds
that may have potential use of drugs. Originally these cell culture tests were done in 96
well plates, but increasing use is now being made of 384 & 1536 well plate.
Corning micro plate
Respiratory cell line
Alveolar cells
A549 cells
Bronchial epithelial cells
BEAS 2B, DMS53, SHP-77,NCI-H23, 292, 524, 727,11655,1299HBE4-E6/E7 etc….. (>140)
Macrophage
Differentiated U937/MonoMac6 cellMurine RAW264.7
• Respiratory cell lines are useful for evaluating of molecular mechanisms of inflammation and other physiological /pathological events.
Pancreatic beta cell lines and their applications in diabetes mellitus research
During the past 30 years great effort has been put into establishing an insulin-
secreting beta cell line that retains normal regulation of insulin secretion, but only few
of these attempts have been successful.
To overcome the limited availability of primary beta cells into the field of diabetes
mellitus research, numerous investigators used X-rays or viruses to induce insulinomas,
in vitro transformation, derivation of cells from transgenic mice or even non-islet cells to
produce immortalized beta cell lines.
The most widely used insulin-secreting cell lines are RIN, HIT, MIN, INS-1 and TC
cells.
These cells produce insulin and small amounts of glucagon and somatostatin.
Despite problems associated with beta cell cultures, these cell lines have provided
some valuable information about physiological processes.
However, an urgent need to establish a "normal" beta cell line of human or pig
origin remains.
Vivalis, a Nantes, France-based biotechnology company, has developed EB66®, a
novel cell line derived from duck embryonic stem cells for the cell culture
production of viral vaccines.
As a result, EB66 cells have become a superior industry alternative for the safe,
cost effective manufacturing of viral vaccines.
Cell line in vaccinesCell lines in vaccines
Vivalis offers research and commercial licenses for this cell line to pharmaceutical
and biotechnology companies for the production of prophylactic and therapeutic
vaccines.