Supplementary Fig. 2. Regulation of mitochondrial biogenesis and representative photomicrographs of confocal microscopy detection for double immunofluorescence of UCP1 and VEGF-A in ingWAT of DJ- 1 KO mice. (A) Transcript levels of indicated genes required for mitochondrial biogenesis (Nrf1, Tfam, CoxIV, and Cox8b) from tissue lysates of ingWAT. (B) Western blot (left) and densitometry analysis (right) of indicated proteins involved in mitochondrial electron transport chain (COX-IV, ATP5B and ETFA) in ingWAT extracts. (C) Representative red MitoTracker staining on ingWAT sections. Nuclei were counterstained with DAPI (blue). Scale bar, 50 µm. (D) Immunoblotting (top) and quantitation (bottom) of indicated proteins implicated in mitochondrial fusion (Mfn1) and fission (Drp1) in ingWAT tissue lysates. (E) Double immunofluorescence detection with antibodies against brown