Supplementary material Cophylogenetic patterns in algal symbionts correlate with repeated symbiont switches during diversification and geographic expansion of lichen-forming fungi in the genus Sticta (Ascomycota, Peltigeraceae) Hanna Lindgren, Bibiana Moncada, Robert Lücking, Nicolas Magain, Antoine Simon, Bernard Goffinet, Emmanuël Sérusiaux, Matthew P. Nelsen, Joel A. Mercado-Díaz, Todd J. Widhelm, H. Thorsten Lumbsch Supplementary Table 1. Specimens included in this study and the Genbank accession codes of their DNA sequences. Species DNA# Locality Voucher Photobiont Photobiont sequences Mycobiont sequences rbcL 18S ITS nuLSU Mcm7 Sticta aff. granatensis 7382 Ecuador Dal Forno 1787b Symbiochloris MT31668 8 MT31440 5 MG367416 MG062990 - S. aff. laciniosa 7226 CostaRica Moncada 5789 Symbiochloris MT31667 6 - MG367401 MG062988 MF984240 S. ainoae UCONN4148 Chile Goffinet 12691 Symbiochloris MT31659 1 MT31436 0 MG457927 MG458140 MT316689 S. caperata LG386 Reunion Sérusiaux Heveochlorella MT31660 9 MT31437 3 MG457929 - - S. caperata LG625 Reunion Sérusiaux Heveochlorella MT31661 0 MT31434 7 MG457934 - - S. caperata LG626 Reunion Sérusiaux Heveochlorella MT31662 0 MT31437 4 MG457935 MT314325 MT316727 S. caperata LG627 Reunion Sérusiaux Heveochlorella MT31662 1 MT31437 5 MG457936 MT314305 MT316728 S. caperata LG962 Reunion Magain & Sérusiaux Heveochlorella MT31661 7 MT31435 8 JQ735979 JQ735996 MT316735 S. caperata LG994 Reunion Magain & Sérusiaux Heveochlorella MT31661 2 MT31438 2 MG457960 MT314311 MT316737 1
17
Embed
ars.els-cdn.com · Web viewSupplementary material Cophylogenetic patterns in algal symbionts correlate with repeated symbiont switches during diversification and geographic expansion
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Supplementary material
Cophylogenetic patterns in algal symbionts correlate with repeated symbiont switches during diversification and geographic expansion of lichen-forming fungi in the genus Sticta (Ascomycota, Peltigeraceae)
Hanna Lindgren, Bibiana Moncada, Robert Lücking, Nicolas Magain, Antoine Simon, Bernard Goffinet, Emmanuël Sérusiaux, Matthew P. Nelsen, Joel A. Mercado-Díaz, Todd J. Widhelm, H. Thorsten Lumbsch
Supplementary Table 1. Specimens included in this study and the Genbank accession codes of their DNA sequences.
Species DNA# Locality Voucher PhotobiontPhotobiont sequences Mycobiont sequencesrbcL 18S ITS nuLSU Mcm7
Sticta aff. granatensis 7382 Ecuador Dal Forno 1787b SymbiochlorisMT31668
8MT31440
5 MG367416 MG062990 -
S. aff. laciniosa 7226 CostaRica Moncada 5789 SymbiochlorisMT31667
6 - MG367401 MG062988 MF984240
S. ainoae UCONN4148 Chile Goffinet 12691 SymbiochlorisMT31659
1MT31436
0 MG457927 MG458140 MT316689
S. caperata LG386 Reunion Sérusiaux HeveochlorellaMT31660
9MT31437
3 MG457929 - -
S. caperata LG625 Reunion Sérusiaux HeveochlorellaMT31661
0MT31434
7 MG457934 - -
S. caperata LG626 Reunion Sérusiaux HeveochlorellaMT31662
0MT31437
4 MG457935 MT314325 MT316727
S. caperata LG627 Reunion Sérusiaux HeveochlorellaMT31662
1MT31437
5 MG457936 MT314305 MT316728
S. caperata LG962 Reunion Magain & Sérusiaux HeveochlorellaMT31661
7MT31435
8 JQ735979 JQ735996 MT316735
S. caperata LG994 Reunion Magain & Sérusiaux HeveochlorellaMT31661
2MT31438
2 MG457960 MT314311 MT316737
S. cinereoglauca 10080 New Zealand de Lange & Baird CH2547 SymbiochlorisMT31668
4MT31440
6 - - -
S. dichotoma LG632 Reunion van den Boom HeveochlorellaMT31661
1MT31435
3 MG457938 MT314306 MT316729
S. dichotoma LG646 Reunion Sérusiaux HeveochlorellaMT31662
4MT31437
7 MG457946 MT314316 MT316731
S. dichotoma LG647 Reunion Sérusiaux HeveochlorellaMT31662
5MT31437
9 MG457947 MT314308 MT316732
1
S. dichotoma LG1005 Reunion Magain & Sérusiaux HeveochlorellaMT31662
9 - MG457963 MG458085 MT316738
S. dichotoma LG1050 Reunion Magain & Sérusiaux HeveochlorellaMT31663
0MT31435
6 MG457966 MT314312 MT316739
S. filix 10079 New Zealand de Lange 12557 ElliptochlorisMT31667
7MT31442
0 MT279736 MT314289 -
S. filix 15075 New Zealand Lücking et al. 38851 ElliptochlorisMT31659
4MT31436
4 MT279728 MT314319 MT316724
S. filix 15076 New Zealand Lücking et al. 39009 ElliptochlorisMT31659
2MT31436
5 MT279729 MT314320 -
S. filix 15077 New Zealand Lücking et al. 38148 ElliptochlorisMT31659
3MT31437
0 MT279730 MT314321 MT316725
S. filix 15078 New Zealand Lücking et al. 38107 ElliptochlorisMT31659
5MT31437
1 MT279731 MT314303 MT316749
S. filix 15079 New Zealand Lücking et al. 38112 ElliptochlorisMT31659
6MT31436
6 MT279727 MT314304 MT316726
S. filix 13785 New Zealand Lücking et al. 38864 ElliptochlorisMT31659
7MT31436
7 MF373770 MT314291 MT316709
S. filix 13790 New Zealand Lücking et al. 38190 Elliptochloris -MT31436
8 MF373775 MT314334 MT316742
S. filix 13792 New Zealand Lücking et al. 39003 ElliptochlorisMT31659
8MT31436
9 MF373777 MT314278 -
S. laciniata 7223 CostaRica Moncada 5778 SymbiochlorisMT31667
8 - MG367399 MG062984 -
S. laciniosa 14985 Cuba Mercado-Díaz CoccomyxaMT31660
1 - - - -
S. laciniosa 14991 Cuba Mercado-Díaz CoccomyxaMT31659
9 - - - -
S. laciniosa 14992 Cuba Mercado-Díaz CoccomyxaMT31660
0 - - - -
S. latifrons 10076 New Zealand Lücking et al. 38763 HeveochlorellaMT31660
2MT31438
7 MF373786 MT314287 MT316707
S. latifrons 13826 New Zealand Lücking et al. 38657 HeveochlorellaMT31667
9MT31439
0 MF373785 MT314279 MT316694
S. latifrons 15059 New Zealand Lücking et al. 38669 HeveochlorellaMT31668
1MT31439
1 MT279738 MT314293 MT316711
S. latifrons 15060 New Zealand Lücking et al. 38447 HeveochlorellaMT31668
2 - MT279739 MT314294 MT316746
S. latifrons 15061 New Zealand Lücking et al. 38662 HeveochlorellaMT31667
0MT31435
7 MT279718 MT314295 MT316712
S. latifrons 15064 New Zealand Lücking et al. 38318 HeveochlorellaMT31666
0MT31434
4 MT279741 MT314336 MT316745
S. latifrons 13822 New Zealand Lücking et al. 38327 HeveochlorellaMT31665
4 - MF373781 MT314328 MT3166932
S. latifrons 15065 New Zealand Lücking et al. 38357 ChloroidiumMT31665
5 - MT279720 MT314299 MT316715
S. latifrons 15073 New Zealand Lücking et al. 38202 ChloroidiumMT31666
4MT31435
9 MT279725 MT314302 MT316722
S. lobarioides 5028 Colombia Alfonso 5 SymbiochlorisMT31668
7 - KC732555 MT314323 MT316755
S. menziesii 13829 New Zealand Lücking et al. 39001 ElliptochlorisMT31665
1MT31439
7 MF373788 MG063014 MF984254
S. menziesii 13834 New Zealand Lücking et al. 39010 ElliptochlorisMT31664
3MT31441
4 MF373793 - MT316754
S. menziesii 13828 New Zealand Lücking et al. 38008 ElliptochlorisMT31660
6MT31439
6 MF373787 MT314280 MT316695
S. menziesii 13833 New Zealand Lücking et al. 38009 ElliptochlorisMT31664
4MT31439
9 MF373792 MT314330 -
S. menziesii 15072 New Zealand Lücking et al. 38048 ElliptochlorisMT31664
7MT31441
2 MT279724 MT314301 MT316721
S. menziesii 13831 New Zealand Lücking et al. 38194 ElliptochlorisMT31664
5MT31439
8 MF373790 MT314282 MT316697
S. menziesii 13830 New Zealand Lücking et al. 38782 ElliptochlorisMT31666
3MT31441
3 MF373789 MT314329 MT316696
S. menziesii 15074 New Zealand Lücking et al. 38941 ElliptochlorisMT31664
8MT31439
4 MT279726 MT314318 MT316723
S. puracensis 6198 Colombia Díaz L1 SymbiochlorisMT31668
5MT31442
1 KC732701 MT314285 MT316756
S. sp. 4676 Colombia Moncada 10475 SymbiochlorisMT31666
6MT31440
2 MT279712 MT314332 MT316702
S. sp. 4677 Colombia Moncada 10473 SymbiochlorisMT31666
7MT31440
3 MT279734 MT314284 MT316703
S. sp. 4691 Colombia Moncada 10580 SymbiochlorisMT31665
3MT31441
7 - - MT316704
S. sp. 4704 Colombia Moncada 10478 SymbiochlorisMT31666
8MT31441
8 - - MT316705
S. sp. 4758 Colombia Moncada 10519 SymbiochlorisMT31666
9MT31440
4 - - MT316706
S. sp. 2 LG4074 Madagascar Sérusiaux HeveochlorellaMT31663
5 - MG457996 MG458107 -
S. sp. 4 LG4083 Madagascar Sérusiaux HeveochlorellaMT31663
7MT31434
9 MG458001 MG458110 MT316750
S. sp. 5 LG4110 Madagascar Sérusiaux HeveochlorellaMT31664
0 - MG458022 MG458120 -
S. sp. 6 LG4084 Madagascar Sérusiaux Heveochlorella -MT31437
6 MG458002 MG458111 MT316751
S. sp. 6 LG4088 Madagascar Sérusiaux HeveochlorellaMT31661
8MT31438
3 MG458006 MT314317 MT3167403
S. sp. 6 LG4102 Madagascar Sérusiaux HeveochlorellaMT31661
6MT31438
4 MG458015 MT314314 MT316752
S. sp. 6 LG4139 Madagascar Sérusiaux HeveochlorellaMT31664
1 - MG458029 MT314315 -
S. sp. 8 LG4109 Madagascar Sérusiaux HeveochlorellaMT31663
9 - MG458021 MG458119 -
S. sp. 10 LG3453 Mauritius Sérusiaux HeveochlorellaMT31663
2 - MG457980 MG458096 -
S. sp. 11 LG640 Reunion Sérusiaux HeveochlorellaMT31661
5MT31438
5 MG457942 - MT316748
S. sp. 11 LG641 Reunion Sérusiaux HeveochlorellaMT31661
9MT31435
4 MG457943 - -
S. sp. 12 LG797 Madagascar Sérusiaux HeveochlorellaMT31662
6MT31438
0 MG457949 MT314309 MT316733
S. sp. 12 LG802 Madagascar Sérusiaux HeveochlorellaMT31662
7MT31435
5 MG457952 MT314310 MT316734
S. sp. 14 LG4066 Madagascar Sérusiaux HeveochlorellaMT31663
3 - MG457989 MG458103 -
S. sp. 19 LG4085 Madagascar Sérusiaux HeveochlorellaMT31663
8 - MG458003 MG458112 -
S. sp. 21 LG4070 Madagascar Sérusiaux HeveochlorellaMT31663
4 - MG457993 MG458105 -
S. sp. 21 LG4077 Madagascar Sérusiaux HeveochlorellaMT31663
6 - MG457998 MG458108 MT316741
S. sp. 25 LG1250 Madagascar Sérusiaux HeveochlorellaMT31661
4MT31434
8 MG457970 - -
S. squamata 13535 New Zealand Lücking et al. 39092 HeveochlorellaMT31666
1 - MT279710 MT314326 MT316690
S. squamata 13534 New Zealand Lücking et al. 39157 ElliptochlorisMT31665
9MT31435
0 MF373762 MT314286 MT316691
S. squamata 15069 New Zealand Lücking et al. 39149 HeveochlorellaMT31665
7MT31434
6 MT279742 MT314298 MT316718
S. stipitata 14461 Australia Lumbsch, Grewe, Widhelm 2174 ElliptochlorisMT31664
9MT31440
7 MT279717 MT314335 -
S. stipitata 14462 Australia Lumbsch, Grewe, Widhelm 2210 ElliptochlorisMT31667
1MT31440
8 MG754197 MG063024 MF984274
S. stipitata 14455 Australia Lumbsch, Grewe, Widhelm 2050a ElliptochlorisMT31660
4MT31436
3 MT279715 MT314288 MT316743
S. stipitata 14457 Australia Lumbsch, Grewe, Widhelm 2078a ElliptochlorisMT31660
5MT31436
1 MT279716 MT314292 MT316710
S. stipitata 14463 Australia Lumbsch, Grewe, Widhelm 2212 ElliptochlorisMT31660
7MT31436
2 MT279737 MT314276 MT316744
S. subcaperata 10084 New Zealand Lücking et al. 38852 ElliptochlorisMT31660
3MT31438
6 MT279714 MT314290 MT3167084
S. subcaperata 15062 New Zealand Lücking et al. 38013 ElliptochlorisMT31667
2MT31439
2 MT279719 MT314296 MT316713
S. subcaperata 15063 New Zealand Lücking et al. 38095 ElliptochlorisMT31667
5MT31440
9 MT279740 MT314297 MT316714
S. subcaperata 13859 New Zealand Lücking et al. 38155 ElliptochlorisMT31668
0MT31440
0 MF373797 MT314331 MT316700
S. subcaperata 13860 New Zealand Lücking et al. 38819 ElliptochlorisMT31668
3MT31441
6 MG754199 MG063023 MT316701
S. subcaperata 13852 New Zealand Lücking et al. 38189 ElliptochlorisMT31667
4MT31441
5 MT279735 MT314281 MT316698
S. subcaperata 13814 New Zealand Lücking et al. 38656 HeveochlorellaMT31668
6MT31437
2 MT279732 MT314277 MT316692
S. subcaperata 13821 New Zealand Lücking et al. 38991 ChloroidiumMT31667
3MT31438
8 MT279733 MT314327 MT316753
S. subcaperata 15066 New Zealand Lücking et al. 38044 ElliptochlorisMT31665
0MT31441
0 MT279709 MT314275 MT316747
S. subcaperata 15067 New Zealand Lücking et al. 38119 ElliptochlorisMT31665
2MT31441
1 MT279743 MT314337 MT316716
S. subcaperata 13853A New Zealand Lücking et al. 38581 HeveochlorellaMT31665
8MT31435
1 MT279711 MT314283 MT316699
S. subcaperata 15068 New Zealand Lücking et al. 38694 HeveochlorellaMT31665
6MT31434
5 MT279721 MT314322 MT316717
S. subcaperata 10077 New Zealand Lücking et al. 38937 ElliptochlorisMT31660
8MT31441
9 MT279713 MT314333 -
S. subcaperata 15070 New Zealand Lücking et al. 38045 ElliptochlorisMT31664
2MT31439
3 MT279722 MT314324 MT316719
S. subcaperata 15071 New Zealand Lücking et al. 38818 ChloroidiumMT31666
2MT31438
9 MT279723 MT314300 MT316720
S. subcaperata 13861 New Zealand Lücking et al. 38949 ElliptochlorisMT31666
5MT31440
1 MG754200 MG063022 MF984273
S. subcaperata 13537 New Zealand Lücking et al. 39061 ElliptochlorisMT31664
6MT31439
5 MG754198 MG063017 MF984231
S.variabilis LG631 Reunion van den Boom HeveochlorellaMT31662
C: cospeciation, D: duplication, D+S: duplication with a host switch, L: loss, FD: failure to diverge.
Supplementary Figure 1. 18S ML phylogeny of algae in Trebouxiophyceae. Highlighted clades represent different genera of algae associated with Sticta. Bootstrap support values ≥75 from ML analysis and Bayesian posterior probabilities ≥95 are shown at nodes.
11
12
Supplementary Figure 2. JANE cophylogeny showing cost scenario G with the lowest total cost. Phylogeny of Sticta shown in black and phylogeny of algal symbiont in blue. Dotted line = loss of symbiont, serrated line= failure of the symbiont to diverge with its host, dot and small arrow = duplication and host switch.