Arbutus, Catlin, Green, Oneida, Onondaga, and Wolf.

Arbutus, Catlin, Green, Oneida, Onondaga, and Wolf.

Presented by

Thomas Clement

&

Amanda Stella

http://www.imagequest3d.com/photos/phytoplankton/s003.jpg

http://www.nies.go.jp/biology/mcc/images/PCD5008/0998L.jpg

Why are phytoplankton important?

• Produce O2 in lakes

• Indicator of primary production

• Control Other populations of organisms

http://www.umanitoba.ca/institutes/fisheries/227_305.jpg

How phytoplankton is measured

• Sampling Techniques– Van Dorn – Preservation with Lugols solution

• Lab Techniques– Water settled for several days– Increased concentration– Identified to Genus

http://www.fisheries.org/education/fisheries_techniques/Chapter4/Removal%20of%20a%20water%20sampler%20from%20a%20Van%20Dorn%20sampler.jpg

Chlorophyll

• Main pigment in photosynthetic organisms

• Commonly used as an indicator of production

http://www.public.asu.edu/~jpbirk/3dstruct/chlorophyll.gif

Chlorophyll Analysis

• Water sampled out in field

• In Lab– Mix sample– Measure sample – Filtered– Packaged in foil– Frozen until analysis

http://www.umass.edu/tei/mwwp/Images/Filt.gif&imgrefurl=http://www.umass.edu/tei/mwwp/chloro.html&h=267&w=247&sz=6&hl=en&start=71&tbnid=DZS0KGvprZR8wM:&tbnh=113&tbnw=105&prev=/images%3Fq%3Dchlorophyll%26start%3D60%26ndsp%3D20%26svnum%3D10%26hl%3Den%26lr%3D%26safe%3Doff%26client%3Dfirefox-%26rls%3Dorg.mozilla:en-US:official%26sa%3DN

Chlorophyll Analysis Con’t

• 90% buffered acetone used for extraction

• Filters added to 15ml alkaline acetone solution

• Refrigerated for 24 hours• Fluorometer was used

– Fluorometer produces a concentration in µgrams/liter

http://cacique.uprm.edu/gers/fluorometer.jpg

Chlorophyll concentrations in the lakes

0

5

10

15

20

25

0 20 40 60 80 100 120 140 160

Chl a (micrograms/liter)

De

pth

(m

)

Onondaga

Catlin

Oneida

Green

Wolf

Arbutus

Phytoplankton Arbutus

012345678

0 2 4 6 8

# of Individuals

Dep

th

Stynura

Mallamonas

Microcystis

Dinobryon

Chlamydomon

Catlin

0

2

4

6

8

10

12

14

0 2 4 6 8 10

# of Individuals

Dep

th

Chrysophyte

Centric Diatom

Flagellate

Tabellaria

Mougeotia

Green

0

5

10

15

20

0 0.5 1 1.5 2 2.5

# of Individuals

Dep

th

Euglenophyta

Oneida

0

2

4

6

8

10

12

0 20 40 60 80 100

# of Individuals

Dep

ths

Aulacosira

Microcystis

Small Flagellates

Mallanomous

Acinastrum

Phytoplankton Con’tOnondaga

02468

1012141618

0 10 20 30 40 50

Depth

# o

f In

div

idu

als

Microcystis

Chrysophyaceae

Actinastrum

Polyblepharides

Bacillariophyceae

Arbutus

012345678

0 2 4 6 8

# of Individuals

Dep

th

Stynura

Mallamonas

Microcystis

Dinobryon

Chlamydomon

Phytoplankton AbundanceArbutus

Catlin

Green

Phytoplankton Abundance Cont’d

OneidaOnondaga

Conclusions

• Cross-Lake Comparisons– Wide variety of phytoplankton

• Sampling Error– Sampling day conditions– Analyzing techniques