AQUACULTURE PATHOLOGY OFFICE PHONE 1-520-621-8414 … · JL Kemang Amarilis 2 Blok AH No. 2 Perumanhan Kemang Pratama 2 Bekesi INDONESIA Case: 08-056 E-mail: [email protected]

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AQUACULTURE PATHOLOGYOFFICE PHONE 1-520-621-8414

LAB PHONE 1-520-621-4438FAX 1-520-621-4899

February 13, 2008Jeff ProchaskaPT Bibit UnggulJL Kemang Amarilis 2Blok AH No. 2Perumanhan Kemang Pratama 2BekesiINDONESIA

Case: 08-028E-mail: [email protected]

Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The 129 pleopods samples from Litopenaeus vannameifor DNA extraction, 123 pleopods for RNA extraction and 300 post-larvae for enteric pathogensarrived on February 11th, 2008 in good condition. The pleopods and post-larvae were pooled ingroups of 5 samples (9 to 50 mg). The samples were collected for DNA and RNAextractions.WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP and IHHNV were Not Detected inany of the samples tested. A summary of the tests and results is provided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $5,592 (26 sets of tests for WSSV/IHHV @ $47.00 each,25 sets of samples for TSV/YHV @ $47.00 each, 25 sets of samples for IMNV @ $21.00 each, 30sets of samples for MBV/HPV @ $47.00, and 60 sets of tests for BP and NHP @ $21.00 each). Aseparate invoice will be sent to you. If there are any questions regarding this case please feel freeto contact us.

UAZ Policy on certification: This report provides our findings on the samples submitted to our laboratory for examination, health status evaluation,disease diagnosis, or pathogen detection. It is our policy and intent to perform the most appropriate assay(s) for the determination of thehealth/pathogen status of all specimens submitted to our laboratory. However, this report in no way constitutes a stock or facility “certification” ora “certificate” of health/pathogen status for the sample(s) tested or for the stocks, or facility, from which the sample(s) were derived.PCR: disclaimer: This report provides our findings on the samples submitted to our laboratory for pathogen detection. The PCR assay used by thislaboratory for the detection of shrimp pathogens is a research tool. The results should be considered as experimental and tentative. Whenever possible,PCR results should be confirmed by alternative assay. This report in no way constitutes a stock or facility “certification” or a “certificate” ofhealth/pathogen status for the sample(s) tested or for the stocks, or facility, from which the sample(s) were derived.

Sincerely yours,

Ms. Solangel Navarro D.V. Lightner Ph.D.Research Specialist Professor

Page 2 of 4

Case: 08-028

Test(s) Requested: Sample Type: Species: YHV 123 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei TSV 123 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei IMNV 123 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei WSSV 129 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei IHHNV 129 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei HPV 300 post larvae in 95% ethanol; pooled in groups of 5 L.vannamei MBV 300 post larvae in 95% ethanol; pooled in groups of 5 L.vannamei NHP 300 post larvae in 95% ethanol; pooled in groups of 5 L.vannamei BP 300 post larvae in 95% ethanol; pooled in groups of 5 L.vannamei

Protocols:YHV: RT- PCR described by Tang and Lightner (DAO, 1999, 35:165-173)TSV: RT-PCR described by Nunan, et al. (DAO, 1998, 34:87-91)WSSV: Two-step nested PCR modified method from Lo, et al (DAO, 1996, 25:133-141)IHHNV: PCR described by Nunan, et al. (Marine Biotechnology, 2000, 2: 319-328)HPV: PCR protocol developed in this laboratoryNHP: 1 step PCR based on Gene bank published sequence U65509BP: PCR protocol developed in this laboratoryMBV: PCR protocol described by Surachetpong et al (Aquaculture, 2005, 249:69-75)IMNV: RT-PCR nested protocol described by Poulos and Lightner (DAO,2006,73:69-72)

Tables:Table 1: 129 pleopdos for DNA pathogens in pools of 5

UAZ # Sample Ref WSSV IHHNV08-028 A1

129 pleopods from PTBibit Unggul

Not Detected Not Detected08-028 A2 Not Detected Not Detected08-028 A3 Not Detected Not Detected08-028 A4 Not Detected Not Detected08-028 A5 Not Detected Not Detected08-028 A6 Not Detected Not Detected08-028 A7 Not Detected Not Detected08-028 A8 Not Detected Not Detected08-028 A9 Not Detected Not Detected08-028 A10 Not Detected Not Detected08-028 A11 Not Detected Not Detected08-028 A12 Not Detected Not Detected08-028 A13 Not Detected Not Detected08-028 A14 Not Detected Not Detected

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UAZ # Sample Ref WSSV IHHNV08-028 A15


Not Detected Not Detected08-028 A16 Not Detected Not Detected08-028 A17 Not Detected Not Detected08-028 A18 Not Detected Not Detected08-028 A19 Not Detected Not Detected08-028 A20 Not Detected Not Detected08-028 A21 Not Detected Not Detected08-028 A22 Not Detected Not Detected08-028 A23 Not Detected Not Detected08-028 A24 Not Detected Not Detected08-028 A25 Not Detected Not Detected08-028 A26 Not Detected Not Detected

Table 2: 123 pleopdos for RNA pathogens in pools of 5UAZ # Sample Ref TSV YHV IMNV

08-028 A1


Not Detected Not Detected Not Detected08-028 A2 Not Detected Not Detected Not Detected08-028 A3 Not Detected Not Detected Not Detected08-028 A4 Not Detected Not Detected Not Detected08-028 A5 Not Detected Not Detected Not Detected08-028 A6 Not Detected Not Detected Not Detected08-028 A7 Not Detected Not Detected Not Detected08-028 A8 Not Detected Not Detected Not Detected08-028 A9 Not Detected Not Detected Not Detected08-028 A10 Not Detected Not Detected Not Detected08-028 A11 Not Detected Not Detected Not Detected08-028 A12 Not Detected Not Detected Not Detected08-028 A13 Not Detected Not Detected Not Detected08-028 A14 Not Detected Not Detected Not Detected08-028 A15 Not Detected Not Detected Not Detected08-028 A16 Not Detected Not Detected Not Detected08-028 A17 Not Detected Not Detected Not Detected08-028 A18 Not Detected Not Detected Not Detected08-028 A19 Not Detected Not Detected Not Detected08-028 A20 Not Detected Not Detected Not Detected08-028 A21 Not Detected Not Detected Not Detected08-028 A22 Not Detected Not Detected Not Detected08-028 A23 Not Detected Not Detected Not Detected08-028 A24 Not Detected Not Detected Not Detected08-028 A25 Not Detected Not Detected Not Detected

Table 3: 150 Post larvae for enteric pathogens in pools of 5

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UAZ # Sample Ref MBV HPV BP NHP08-028 B1

300 Post-larvaefrom

PT BibitUnggul

Not Detected Not Detected Not Detected Not Detected08-028 B2 Not Detected Not Detected Not Detected Not Detected08-028 B3 Not Detected Not Detected Not Detected Not Detected08-028 B4 Not Detected Not Detected Not Detected Not Detected08-028 B5 Not Detected Not Detected Not Detected Not Detected08-028 B6 Not Detected Not Detected Not Detected Not Detected08-028 B7 Not Detected Not Detected Not Detected Not Detected08-028 B8 Not Detected Not Detected Not Detected Not Detected08-028 B9 Not Detected Not Detected Not Detected Not Detected08-028 B10 Not Detected Not Detected Not Detected Not Detected08-028 B11 Not Detected Not Detected Not Detected Not Detected08-028 B12 Not Detected Not Detected Not Detected Not Detected08-028 B13 Not Detected Not Detected Not Detected Not Detected08-028 B14 Not Detected Not Detected Not Detected Not Detected08-028 B15 Not Detected Not Detected Not Detected Not Detected08-028 B16 Not Detected Not Detected Not Detected Not Detected08-028 B17 Not Detected Not Detected Not Detected Not Detected08-028 B18 Not Detected Not Detected Not Detected Not Detected08-028 B19 Not Detected Not Detected Not Detected Not Detected08-028 B20 Not Detected Not Detected Not Detected Not Detected08-028 B21 Not Detected Not Detected Not Detected Not Detected08-028 B22 Not Detected Not Detected Not Detected Not Detected08-028 B23 Not Detected Not Detected Not Detected Not Detected08-028 B24 Not Detected Not Detected Not Detected Not Detected08-028 B25 Not Detected Not Detected Not Detected Not Detected08-028 B26 Not Detected Not Detected Not Detected Not Detected08-028 B27 Not Detected Not Detected Not Detected Not Detected08-028 B28 Not Detected Not Detected Not Detected Not Detected08-028 B29 Not Detected Not Detected Not Detected Not Detected08-028 B30 Not Detected Not Detected Not Detected Not Detected

Conclusion: WSSV, TSV, YHV, IMNNV, MBV, BP, HPV, NHP and IHHNV were NotDetected in any of he samples tested.

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LAB PHONE 1-520-621-4438FAX 1-520-621-4899

March 31, 2008Jeff ProchaskaPT Bibit UnggulJL Kemang Amarilis 2Blok AH No. 2Perumanhan Kemang Pratama 2BekesiINDONESIA

Case: 08-056E-mail: [email protected] Related Case: 08-028

Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, IMNV, YHV & TSV by PCRhave been completed. The 30 pleopods samples from Litopenaeus vannamei for DNA extractionand 30 pleopods for RNA extraction arrived on March 24th, 2008 in good condition. The pleopodswere pooled in groups of 5 samples (31 to 50 mg). The samples were collected for DNA and RNAextractions. This samples were submitted to complete the tests reported previously on UAZ Case08-028. WSSV, TSV, YHV, IMNV and IHHNV were Not Detected in any of the samples tested.A summary of the tests and results is provided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $622 (6 sets of tests for WSSV/IHHV @ $47.00 each, 5 setsof samples for TSV/YHV @ $47.00 each, 5 sets of samples for IMNV @ $21.00 each). A separateinvoice will be sent to you. If there are any questions regarding this case please feel free to contactus.


Sincerely yours,


Page 2 of 2

Case: 08-056Related Case: 08-028

Test(s) Requested: Sample Type: Species: YHV 30 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei TSV 30 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei IMNV 30 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei WSSV 30 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei IHHNV 30 pleopods in 95% ethanol; pooled in groups of 5 L.vannamei

Protocols:YHV: RT- PCR described by Tang and Lightner (DAO, 1999, 35:165-173)TSV: RT-PCR described by Nunan, et al. (DAO, 1998, 34:87-91)WSSV: Two-step nested PCR modified method from Lo, et al (DAO, 1996, 25:133-141)IHHNV: PCR described by Nunan, et al. (Marine Biotechnology, 2000, 2: 319-328)IMNV: RT-PCR nested protocol described by Poulos and Lightner (DAO,2006,73:69-72)

Tables:UAZ # Sample Ref WSSV IHHV TSV YHV IMNV

08-056/ 1

Quarantine Facilityin LombokIndonesia

NotDetected

NotDetected

NotDetected

NotDetected

NotDetected

08-056/ 2 NotDetected

NotDetected

NotDetected

NotDetected

NotDetected


NotDetected

NotDetected

NotDetected

NotDetected


NotDetected

NotDetected

NotDetected

NotDetected


NotDetected

NotDetected

NotDetected

NotDetected


NotDetected ------- ------- -------

Conclusion: WSSV, TSV, YHV, IMNNV and IHHNV were Not Detected in any of hesamples tested.

Page 1 of 6


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

September 19, 2008Jeff ProchaskaPT Bibit UnggulJL Kemang Amarilis 2Blok AH No. 2Perumanhan Kemang Pratama 2BekesiINDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The 605 pleopods, 300 postlarvae, 5 tail segments, 5hepatopancreas and 5 fecal strands samples from Litopenaeus vannamei on arrived on September9th, 2008 in good condition. All of the samples were pooled in groups of 5 samples (10 to 50 mg)per your instructions. The samples were collected for DNA and RNA extractions. WSSV, TSV,YHV, IMNV, MBV, BP, HPV, NHP and IHHNV were Not Detected in any of the samples tested.A summary of the tests and results is provided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $12,648.00 (62 sets of tests for WSSV/IHHV @ $47.00each, 62 sets of samples for TSV/YHV @ $47.00 each, 62 sets of samples for IMNV @ $21.00each, 62 sets of samples for MBV/HPV @ $47.00, and 124 sets of tests for BP and NHP @ $21.00each). A separate invoice will be sent to you. If there are any questions regarding this case pleasefeel free to contact us.


Sincerely yours,


Page 2 of 6

Case: 08-207

Test(s) Requested: Sample Type: Species:

YHV 310 pleopods or tissue samples in 95% ethanol; pooled in groups of 5 L. vannamei TSV 310 pleopods or tissue samples in 95% ethanol; pooled in groups of 5 L. vannamei IMNV 310 pleopods or tissue samples in 95% ethanol; pooled in groups of 5 L. vannamei WSSV 310 pleopods or tissue samples in 95% ethanol; pooled in groups of 5 L. vannamei IHHNV 310 pleopods or tissue samples in 95% ethanol; pooled in groups of 5 L. vannamei HPV 310 post larvae, hepatopancreas or fecal strands in 95% ethanol; pooled in groups of 5 L. vannamei MBV 310 post larvae, hepatopancreas or fecal strands in 95% ethanol; pooled in groups of 5 L. vannamei NHP 310 post larvae, hepatopancreas or fecal strands in 95% ethanol; pooled in groups of 5 L. vannamei BP 310 post larvae, hepatopancreas or fecal strands in 95% ethanol; pooled in groups of 5 L. vannamei

Protocols:

YHV: RT- PCR described by Tang and Lightner (DAO, 1999, 35:165-173)TSV: RT-PCR described by Nunan, et al. (DAO, 1998, 34:87-91)WSSV: Two-step nested PCR modified method from Lo, et al (DAO, 1996, 25:133-141)IHHNV: PCR described by Nunan, et al. (Marine Biotechnology, 2000, 2: 319-328)HPV: PCR described by Tang, et al. (DAO, 2008, 80:105-112)NHP: 1 step PCR based on Gene bank published sequence U65509BP: PCR protocol developed in this laboratoryMBV: PCR protocol described by Surachetpong et al (Aquaculture, 2005, 249:69-75)IMNV: RT-PCR nested protocol described by Poulos and Lightner (DAO,2006,73:69-72)

Page 3 of 6

Tables:Table 1: 300 pleopods from NBC and 5 tail segments from NBC Juveniles

UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV08-207 A1

NBC plepods

150 for DNASystemicpathogens

150 for RNASystemicpathogens

Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A2 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A3 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A4 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A5 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A6 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A7 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A8 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A9 Not Detected Not Detected Not Detected Not Detected Not Detected

08-207 A10 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A11 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A12 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A13 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A14 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A15 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A16 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A17 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A18 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A19 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A20 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A21 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A22 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A23 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A24 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A25 Not Detected Not Detected Not Detected Not Detected Not Detected08-207 A26 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ A27 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ A28 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ A29 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ A30 Not Detected Not Detected Not Detected Not Detected Not Detected

08-207/ C NBC juveniles5 tail segments Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 6

Table 2: 300 whole Post larvae from NBC and 5 hepatopancreas from NBC JuvenilesUAZ # Sample Ref MBV HPV BP NHP

08-207/ B1

NBC Postlarvae

300 wholePostlarvae for

DNAEnteric pathogens

Not Detected Not Detected Not Detected Not Detected08-207/ B2 Not Detected Not Detected Not Detected Not Detected08-207/ B3 Not Detected Not Detected Not Detected Not Detected08-207/ B4 Not Detected Not Detected Not Detected Not Detected08-207/ B5 Not Detected Not Detected Not Detected Not Detected08-207/ B6 Not Detected Not Detected Not Detected Not Detected08-207/ B7 Not Detected Not Detected Not Detected Not Detected08-207/ B8 Not Detected Not Detected Not Detected Not Detected08-207/ B9 Not Detected Not Detected Not Detected Not Detected

08-207/ B10 Not Detected Not Detected Not Detected Not Detected08-207/ B11 Not Detected Not Detected Not Detected Not Detected08-207/ B12 Not Detected Not Detected Not Detected Not Detected08-207/ B13 Not Detected Not Detected Not Detected Not Detected08-207/ B14 Not Detected Not Detected Not Detected Not Detected08-207/ B15 Not Detected Not Detected Not Detected Not Detected08-207/ B16 Not Detected Not Detected Not Detected Not Detected08-207/ B17 Not Detected Not Detected Not Detected Not Detected08-207/ B18 Not Detected Not Detected Not Detected Not Detected08-207/ B19 Not Detected Not Detected Not Detected Not Detected08-207/ B20 Not Detected Not Detected Not Detected Not Detected08-207/ B21 Not Detected Not Detected Not Detected Not Detected08-207/ B22 Not Detected Not Detected Not Detected Not Detected08-207/ B23 Not Detected Not Detected Not Detected Not Detected08-207/ B24 Not Detected Not Detected Not Detected Not Detected08-207/ B25 Not Detected Not Detected Not Detected Not Detected08-207/ B26 Not Detected Not Detected Not Detected Not Detected08-207/ B27 Not Detected Not Detected Not Detected Not Detected08-207/ B28 Not Detected Not Detected Not Detected Not Detected08-207/ B29 Not Detected Not Detected Not Detected Not Detected08-207/ B30 Not Detected Not Detected Not Detected Not Detected

08-207/ C NBC juveniles5 Hepatopancreas Not Detected Not Detected Not Detected Not Detected

Page 5 of 6

Table 3: 300 pleopods from BMC and 5 pleopods from QuarantineUAZ # Sample Ref WSSV IHHNV TSV YHV IMNV

08-207/ D1

BMC plepods

150 for DNASystemicpathogens

150 for RNASystemicpathogens

Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D2 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D3 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D4 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D5 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D6 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D7 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D8 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D9 Not Detected Not Detected Not Detected Not Detected Not Detected

08-207/ D10 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D11 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D12 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D13 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D14 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D15 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D16 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D17 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D18 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D19 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D20 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D21 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D22 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D23 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D24 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D25 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D26 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D27 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D28 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D29 Not Detected Not Detected Not Detected Not Detected Not Detected08-207/ D30 Not Detected Not Detected Not Detected Not Detected Not Detected

08-207/ F Quarantine5 pleopods Not Detected Not Detected Not Detected Not Detected Not Detected

Page 6 of 6

Table 4: 300 whole Post larvae from BMC and 5 fecal strands from QuarantineUAZ # Sample Ref MBV HPV BP NHP

08-207/ E1

BMC Postlarvae

300 wholePostlarvae for

DNAEnteric pathogens

Not Detected Not Detected Not Detected Not Detected08-207/ E2 Not Detected Not Detected Not Detected Not Detected08-207/ E3 Not Detected Not Detected Not Detected Not Detected08-207/ E4 Not Detected Not Detected Not Detected Not Detected08-207/ E5 Not Detected Not Detected Not Detected Not Detected08-207/ E6 Not Detected Not Detected Not Detected Not Detected08-207/ E7 Not Detected Not Detected Not Detected Not Detected08-207/ E8 Not Detected Not Detected Not Detected Not Detected08-207/ E9 Not Detected Not Detected Not Detected Not Detected

08-207/ E10 Not Detected Not Detected Not Detected Not Detected08-207/ E11 Not Detected Not Detected Not Detected Not Detected08-207/ E12 Not Detected Not Detected Not Detected Not Detected08-207/ E13 Not Detected Not Detected Not Detected Not Detected08-207/ E14 Not Detected Not Detected Not Detected Not Detected08-207/ E15 Not Detected Not Detected Not Detected Not Detected08-207/ E16 Not Detected Not Detected Not Detected Not Detected08-207/ E17 Not Detected Not Detected Not Detected Not Detected08-207/ E18 Not Detected Not Detected Not Detected Not Detected08-207/ E19 Not Detected Not Detected Not Detected Not Detected08-207/ E20 Not Detected Not Detected Not Detected Not Detected08-207/ E21 Not Detected Not Detected Not Detected Not Detected08-207/ E22 Not Detected Not Detected Not Detected Not Detected08-207/ E23 Not Detected Not Detected Not Detected Not Detected08-207/ E24 Not Detected Not Detected Not Detected Not Detected08-207/ E25 Not Detected Not Detected Not Detected Not Detected-08-207/ E26 Not Detected Not Detected Not Detected Not Detected08-207/ E27 Not Detected Not Detected Not Detected Not Detected08-207/ E28 Not Detected Not Detected Not Detected Not Detected08-207/ E29 Not Detected Not Detected Not Detected Not Detected08-207/ E30 Not Detected Not Detected Not Detected Not Detected

08-207/ F Quarantine5 Fecal strands Not Detected Not Detected Not Detected Not Detected

Conclusion: WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP and IHHNV were Not Detected in any of the samples tested

Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

February 16, 2009Jeff ProchaskaPT Bibit UnggulJL. Alternatif CibuburKomp. Cibubur Point Blok B 12 ARt. 05 RW. 09, HarjamuktiCimanggis, Depok 16954INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei on arrived on February 6th, 2009 in good condition. All of the samples were pooled ingroups of 5 samples (20 to 50 mg) per your instructions. The samples were collected for DNA andRNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP and IHHNV were NotDetected in any of the samples tested. A summary of the tests and results is provided on thefollowing page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $6,120.00 (30 sets of tests for WSSV/IHHV @ $47.00 each,30 sets of samples for TSV/YHV @ $47.00 each, 30 sets of samples for IMNV @ $21.00 each, 30sets of samples for MBV/HPV @ $47.00, and 60 sets of tests for BP and NHP @ $21.00 each). Aseparate invoice will be sent to you. If there are any questions regarding this case please feel freeto contact us.


Sincerely yours,

Ms. Solangel Navarro Carlos R. PantojaResearch Specialist Associate Research Professor

Page 2 of 4

Case: 09-033


YHV 150 pleopods in 95% ethanol; pooled in groups of 5 L. vannamei TSV 150 pleopods in 95% ethanol; pooled in groups of 5 L. vannamei IMNV 150 pleopods in 95% ethanol; pooled in groups of 5 L. vannamei WSSV 150 pleopods in 95% ethanol; pooled in groups of 5 L. vannamei IHHNV 150 pleopods in 95% ethanol; pooled in groups of 5 L. vannamei HPV 150 post larvae in 95% ethanol; pooled in groups of 5 L. vannamei MBV 150 post larvae in 95% ethanol; pooled in groups of 5 L. vannamei NHP 150 post larvae in 95% ethanol; pooled in groups of 5 L. vannamei BP 150 post larvae in 95% ethanol; pooled in groups of 5 L. vannamei

Protocols:

YHV: RT- PCR described by Tang and Lightner (DAO, 1999, 35:165-173)TSV: RT-PCR described by Nunan, et al. (DAO, 1998, 34:87-91)WSSV: Two-step nested PCR modified method from Lo, et al (DAO, 1996, 25:133-141)IHHNV: PCR described by Nunan, et al. (Marine Biotechnology, 2000, 2: 319-328)HPV: PCR described by Tang, et al. (DAO, 2008, 80:105-112)NHP: PCR described by Nunan, et al. (DAO, 2008, 80:64-73)BP: PCR protocol developed in this laboratoryMBV: PCR protocol described by Surachetpong et al (Aquaculture, 2005, 249:69-75)IMNV: RT-PCR nested protocol described by Poulos and Lightner (DAO,2006,73:69-72)

Page 3 of 4

Table 1: 300 pleopods from BMC

UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV09-033/ 1

BMC plepods

150 pleopods ingroup of 5 forDNA Systemic

pathogens

150 pleopods ingroups of 5 forRNA Systemic

pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 2 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 3 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 4 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 5 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 6 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 7 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 8 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 9 Not Detected Not Detected Not Detected Not Detected Not Detected

09-033/ 10 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 11 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 12 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 13 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 14 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 15 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 16 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 17 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 18 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 19 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 20 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 21 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 22 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 23 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 24 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 25 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 26 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 27 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 28 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 29 Not Detected Not Detected Not Detected Not Detected Not Detected09-033/ 30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4

Table 2: 150 whole Post larvae from BMC

UAZ # Sample Ref MBV HPV BP NHP09-033/ 1

BMC Postlarvae

150 Post larvae ingroups of 5 forDNA Enteric pathogens

Not Detected Not Detected Not Detected Not Detected09-033/ 2 Not Detected Not Detected Not Detected Not Detected09-033/ 3 Not Detected Not Detected Not Detected Not Detected09-033/ 4 Not Detected Not Detected Not Detected Not Detected09-033/ 5 Not Detected Not Detected Not Detected Not Detected09-033/ 6 Not Detected Not Detected Not Detected Not Detected09-033/ 7 Not Detected Not Detected Not Detected Not Detected09-033/ 8 Not Detected Not Detected Not Detected Not Detected09-033/ 9 Not Detected Not Detected Not Detected Not Detected

09-033/ 10 Not Detected Not Detected Not Detected Not Detected09-033/ 11 Not Detected Not Detected Not Detected Not Detected09-033/ 12 Not Detected Not Detected Not Detected Not Detected09-033/ 13 Not Detected Not Detected Not Detected Not Detected09-033/ 14 Not Detected Not Detected Not Detected Not Detected09-033/ 15 Not Detected Not Detected Not Detected Not Detected09-033/ 16 Not Detected Not Detected Not Detected Not Detected09-033/ 17 Not Detected Not Detected Not Detected Not Detected09-033/ 18 Not Detected Not Detected Not Detected Not Detected09-033/ 19 Not Detected Not Detected Not Detected Not Detected09-033/ 20 Not Detected Not Detected Not Detected Not Detected09-033/ 21 Not Detected Not Detected Not Detected Not Detected09-033/ 22 Not Detected Not Detected Not Detected Not Detected09-033/ 23 Not Detected Not Detected Not Detected Not Detected09-033/ 24 Not Detected Not Detected Not Detected Not Detected09-033/ 25 Not Detected Not Detected Not Detected Not Detected09-033/ 26 Not Detected Not Detected Not Detected Not Detected09-033/ 27 Not Detected Not Detected Not Detected Not Detected09-033/ 28 Not Detected Not Detected Not Detected Not Detected09-033/ 29 Not Detected Not Detected Not Detected Not Detected09-033/ 30 Not Detected Not Detected Not Detected Not Detected


Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

February 16, 2009Jeff ProchaskaPT Bibit UnggulJL. Alternatif CibuburKomp. Cibubur Point Blok B 12 ARt. 05 RW. 09, HarjamuktiCimanggis, Depok 16954INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei on arrived on February 6th, 2009 in good condition. All of the samples were pooled ingroups of 5 samples (20 to 50 mg) per your instructions. The samples were collected for DNA andRNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP and IHHNV were NotDetected in any of the samples tested. A summary of the tests and results is provided on thefollowing page.



Sincerely yours,


Page 2 of 4

Case: 09-034



Protocols:


Page 3 of 4

Table 1: 300 pleopods from NBC

UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV09-034/ 1

NBC plepods


pathogens


pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 2 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 3 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 4 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 5 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 6 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 7 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 8 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 9 Not Detected Not Detected Not Detected Not Detected Not Detected

09-034/ 10 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 11 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 12 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 13 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 14 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 15 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 16 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 17 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 18 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 19 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 20 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 21 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 22 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 23 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 24 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 25 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 26 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 27 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 28 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 29 Not Detected Not Detected Not Detected Not Detected Not Detected09-034/ 30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4

Table 2: 150 whole Post larvae from NBC

UAZ # Sample Ref MBV HPV BP NHP09-034/ 1

NBC Postlarvae


Not Detected Not Detected Not Detected Not Detected09-034/ 2 Not Detected Not Detected Not Detected Not Detected09-034/ 3 Not Detected Not Detected Not Detected Not Detected09-034/ 4 Not Detected Not Detected Not Detected Not Detected09-034/ 5 Not Detected Not Detected Not Detected Not Detected09-034/ 6 Not Detected Not Detected Not Detected Not Detected09-034/ 7 Not Detected Not Detected Not Detected Not Detected09-034/ 8 Not Detected Not Detected Not Detected Not Detected09-034/ 9 Not Detected Not Detected Not Detected Not Detected

09-034/ 10 Not Detected Not Detected Not Detected Not Detected09-034/ 11 Not Detected Not Detected Not Detected Not Detected09-034/ 12 Not Detected Not Detected Not Detected Not Detected09-034/ 13 Not Detected Not Detected Not Detected Not Detected09-034/ 14 Not Detected Not Detected Not Detected Not Detected09-034/ 15 Not Detected Not Detected Not Detected Not Detected09-034/ 16 Not Detected Not Detected Not Detected Not Detected09-034/ 17 Not Detected Not Detected Not Detected Not Detected09-034/ 18 Not Detected Not Detected Not Detected Not Detected09-034/ 19 Not Detected Not Detected Not Detected Not Detected09-034/ 20 Not Detected Not Detected Not Detected Not Detected09-034/ 21 Not Detected Not Detected Not Detected Not Detected09-034/ 22 Not Detected Not Detected Not Detected Not Detected09-034/ 23 Not Detected Not Detected Not Detected Not Detected09-034/ 24 Not Detected Not Detected Not Detected Not Detected09-034/ 25 Not Detected Not Detected Not Detected Not Detected09-034/ 26 Not Detected Not Detected Not Detected Not Detected09-034/ 27 Not Detected Not Detected Not Detected Not Detected09-034/ 28 Not Detected Not Detected Not Detected Not Detected09-034/ 29 Not Detected Not Detected Not Detected Not Detected09-034/ 30 Not Detected Not Detected Not Detected Not Detected


Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

September 1, 2009Jeff ProchaskaPT Bibit UnggulJL. Alternatif CibuburKomp. Cibubur Point Blok B 12 ARt. 05 RW. 09, HarjamuktiCimanggis, Depok 16954INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei from the BMC facility arrived on August 12th, 2009 in good condition. All of the sampleswere pooled in groups of 5 samples (10 to 35 mg) per your instructions. The samples werecollected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP andIHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.



Sincerely yours,


Page 2 of 4

Case: 09-219



Protocols:


Page 3 of 4


UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV09-219/ E1

BMC plepods


pathogens


pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E2 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E3 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E4 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E5 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E6 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E7 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E8 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E9 Not Detected Not Detected Not Detected Not Detected Not Detected

09-219/ E10 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E11 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E12 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E13 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E14 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E15 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E16 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E17 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E18 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E19 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E20 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E21 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E22 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E23 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E24 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E25 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E26 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E27 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E28 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E29 Not Detected Not Detected Not Detected Not Detected Not Detected09-219/ E30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4


UAZ # Sample Ref MBV HPV BP NHP09-219/ F1

BMC Postlarvae


Not Detected Not Detected Not Detected Not Detected09-219/ F2 Not Detected Not Detected Not Detected Not Detected09-219/ F3 Not Detected Not Detected Not Detected Not Detected09-219/ F4 Not Detected Not Detected Not Detected Not Detected09-219/ F5 Not Detected Not Detected Not Detected Not Detected09-219/ F6 Not Detected Not Detected Not Detected Not Detected09-219/ F7 Not Detected Not Detected Not Detected Not Detected09-219/ F8 Not Detected Not Detected Not Detected Not Detected09-219/ F9 Not Detected Not Detected Not Detected Not Detected

09-219/ F10 Not Detected Not Detected Not Detected Not Detected09-219/ F11 Not Detected Not Detected Not Detected Not Detected09-219/ F12 Not Detected Not Detected Not Detected Not Detected09-219/ F13 Not Detected Not Detected Not Detected Not Detected09-219/ F14 Not Detected Not Detected Not Detected Not Detected09-219/ F15 Not Detected Not Detected Not Detected Not Detected09-219/ F16 Not Detected Not Detected Not Detected Not Detected09-219/ F17 Not Detected Not Detected Not Detected Not Detected09-219/ F18 Not Detected Not Detected Not Detected Not Detected09-219/ F19 Not Detected Not Detected Not Detected Not Detected09-219/ F20 Not Detected Not Detected Not Detected Not Detected09-219/ F21 Not Detected Not Detected Not Detected Not Detected09-219/ F22 Not Detected Not Detected Not Detected Not Detected09-219/ F23 Not Detected Not Detected Not Detected Not Detected09-219/ F24 Not Detected Not Detected Not Detected Not Detected09-219/ F25 Not Detected Not Detected Not Detected Not Detected09-219/ F26 Not Detected Not Detected Not Detected Not Detected09-219/ F27 Not Detected Not Detected Not Detected Not Detected09-219/ F28 Not Detected Not Detected Not Detected Not Detected09-219/ F29 Not Detected Not Detected Not Detected Not Detected09-219/ F30 Not Detected Not Detected Not Detected Not Detected


Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

September 1, 2009Jeff ProchaskaPT Bibit UnggulJL. Alternatif CibuburKomp. Cibubur Point Blok B 12 ARt. 05 RW. 09, HarjamuktiCimanggis, Depok 16954INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei from the NBC facility arrived on August 12th, 2009 in good condition. All of the sampleswere pooled in groups of 5 samples (10 to 35 mg) per your instructions. The samples werecollected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHP andIHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.



Sincerely yours,


Page 2 of 4

Case: 09-220



Protocols:


Page 3 of 4


UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV09-220/ G1

NBC plepods


pathogens


pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G2 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G3 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G4 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G5 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G6 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G7 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G8 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G9 Not Detected Not Detected Not Detected Not Detected Not Detected

09-220/ G10 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G11 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G12 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G13 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G14 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G15 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G16 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G17 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G18 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G19 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G20 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G21 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G22 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G23 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G24 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G25 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G26 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G27 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G28 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G29 Not Detected Not Detected Not Detected Not Detected Not Detected09-220/ G30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4


UAZ # Sample Ref MBV HPV BP NHP09-220/ H1

NBC Postlarvae


Not Detected Not Detected Not Detected Not Detected09-220/ H2 Not Detected Not Detected Not Detected Not Detected09-220/ H3 Not Detected Not Detected Not Detected Not Detected09-220/ H4 Not Detected Not Detected Not Detected Not Detected09-220/ H5 Not Detected Not Detected Not Detected Not Detected09-220/ H6 Not Detected Not Detected Not Detected Not Detected09-220/ H7 Not Detected Not Detected Not Detected Not Detected09-220/ H8 Not Detected Not Detected Not Detected Not Detected09-220/ H9 Not Detected Not Detected Not Detected Not Detected

09-220/ H10 Not Detected Not Detected Not Detected Not Detected09-220/ H11 Not Detected Not Detected Not Detected Not Detected09-220/ H12 Not Detected Not Detected Not Detected Not Detected09-220/ H13 Not Detected Not Detected Not Detected Not Detected09-220/ H14 Not Detected Not Detected Not Detected Not Detected09-220/ H15 Not Detected Not Detected Not Detected Not Detected09-220/ H16 Not Detected Not Detected Not Detected Not Detected09-220/ H17 Not Detected Not Detected Not Detected Not Detected09-220/ H18 Not Detected Not Detected Not Detected Not Detected09-220/ H19 Not Detected Not Detected Not Detected Not Detected09-220/ H20 Not Detected Not Detected Not Detected Not Detected09-220/ H21 Not Detected Not Detected Not Detected Not Detected09-220/ H22 Not Detected Not Detected Not Detected Not Detected09-220/ H23 Not Detected Not Detected Not Detected Not Detected09-220/ H24 Not Detected Not Detected Not Detected Not Detected09-220/ H25 Not Detected Not Detected Not Detected Not Detected09-220/ H26 Not Detected Not Detected Not Detected Not Detected09-220/ H27 Not Detected Not Detected Not Detected Not Detected09-220/ H28 Not Detected Not Detected Not Detected Not Detected09-220/ H29 Not Detected Not Detected Not Detected Not Detected09-220/ H30 Not Detected Not Detected Not Detected Not Detected


Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

March 16, 2010Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei from the PT BUBMC facility arrived on February 19th, 2010 in good condition. All ofthe samples were pooled in groups of 5 samples (10 to 35 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHPand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.



Sincerely yours,


Page 2 of 4

Case: 10-062



Protocols:


Page 3 of 4


UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV10-062/ D1

PT BUBMC plepods


pathogens


pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D2 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D3 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D4 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D5 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D6 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D7 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D8 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D9 Not Detected Not Detected Not Detected Not Detected Not Detected

10-062/ D10 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D11 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D12 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D13 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D14 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D15 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D16 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D17 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D18 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D19 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D20 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D21 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D22 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D23 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D24 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D25 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D26 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D27 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D28 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D29 Not Detected Not Detected Not Detected Not Detected Not Detected10-062/ D30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4


UAZ # Sample Ref MBV HPV BP NHP10-062/ C1

PT BUBMC

Postlarvae


Not Detected Not Detected Not Detected Not Detected10-062/ C2 Not Detected Not Detected Not Detected Not Detected10-062/ C3 Not Detected Not Detected Not Detected Not Detected10-062/ C4 Not Detected Not Detected Not Detected Not Detected10-062/ C5 Not Detected Not Detected Not Detected Not Detected10-062/ C6 Not Detected Not Detected Not Detected Not Detected10-062/ C7 Not Detected Not Detected Not Detected Not Detected10-062/ C8 Not Detected Not Detected Not Detected Not Detected10-062/ C9 Not Detected Not Detected Not Detected Not Detected

10-062/ C10 Not Detected Not Detected Not Detected Not Detected10-062/ C11 Not Detected Not Detected Not Detected Not Detected10-062/ C12 Not Detected Not Detected Not Detected Not Detected10-062/ C13 Not Detected Not Detected Not Detected Not Detected10-062/ C14 Not Detected Not Detected Not Detected Not Detected10-062/ C15 Not Detected Not Detected Not Detected Not Detected10-062/ C16 Not Detected Not Detected Not Detected Not Detected10-062/ C17 Not Detected Not Detected Not Detected Not Detected10-062/ C18 Not Detected Not Detected Not Detected Not Detected10-062/ C19 Not Detected Not Detected Not Detected Not Detected10-062/ C20 Not Detected Not Detected Not Detected Not Detected10-062/ C21 Not Detected Not Detected Not Detected Not Detected10-062/ C22 Not Detected Not Detected Not Detected Not Detected10-062/ C23 Not Detected Not Detected Not Detected Not Detected10-062/ C24 Not Detected Not Detected Not Detected Not Detected10-062/ C25 Not Detected Not Detected Not Detected Not Detected10-062/ C26 Not Detected Not Detected Not Detected Not Detected10-062/ C27 Not Detected Not Detected Not Detected Not Detected10-062/ C28 Not Detected Not Detected Not Detected Not Detected10-062/ C29 Not Detected Not Detected Not Detected Not Detected10-062/ C30 Not Detected Not Detected Not Detected Not Detected


Page 1 of 4


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

March 16, 2010Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA


Dear Mr. Prochaska:

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The150 postlarvae and 300 pleopods from Litopenaeusvannamei from the PT BU NBC facility arrived on February 19th, 2010 in good condition. All ofthe samples were pooled in groups of 5 samples (10 to 35 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HPV, NHPand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.



Sincerely yours,


Page 2 of 4

Case: 10-061



Protocols:


Page 3 of 4


UAZ # Sample Ref WSSV IHHNV TSV YHV IMNV10-061/ G1

PT BUNBC plepods


pathogens


pathogens

Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G2 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G3 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G4 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G5 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G6 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G7 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G8 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G9 Not Detected Not Detected Not Detected Not Detected Not Detected

10-061/ G10 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G11 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G12 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G13 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G14 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G15 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G16 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G17 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G18 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G19 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G20 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G21 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G22 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G23 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G24 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G25 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G26 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G27 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G28 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G29 Not Detected Not Detected Not Detected Not Detected Not Detected10-061/ G30 Not Detected Not Detected Not Detected Not Detected Not Detected

Page 4 of 4


UAZ # Sample Ref MBV HPV BP NHP10-061/ H1

PT BUNBC

Postlarvae


Not Detected Not Detected Not Detected Not Detected10-061/ H2 Not Detected Not Detected Not Detected Not Detected10-061/ H3 Not Detected Not Detected Not Detected Not Detected10-061/ H4 Not Detected Not Detected Not Detected Not Detected10-061/ H5 Not Detected Not Detected Not Detected Not Detected10-061/ H6 Not Detected Not Detected Not Detected Not Detected10-061/ H7 Not Detected Not Detected Not Detected Not Detected10-061/ H8 Not Detected Not Detected Not Detected Not Detected10-061/ H9 Not Detected Not Detected Not Detected Not Detected

10-061/ H10 Not Detected Not Detected Not Detected Not Detected10-061/ H11 Not Detected Not Detected Not Detected Not Detected10-061/ H12 Not Detected Not Detected Not Detected Not Detected10-061/ H13 Not Detected Not Detected Not Detected Not Detected10-061/ H14 Not Detected Not Detected Not Detected Not Detected10-061/ H15 Not Detected Not Detected Not Detected Not Detected10-061/ H16 Not Detected Not Detected Not Detected Not Detected10-061/ H17 Not Detected Not Detected Not Detected Not Detected10-061/ H18 Not Detected Not Detected Not Detected Not Detected10-061/ H19 Not Detected Not Detected Not Detected Not Detected10-061/ H20 Not Detected Not Detected Not Detected Not Detected10-061/ H21 Not Detected Not Detected Not Detected Not Detected10-061/ H22 Not Detected Not Detected Not Detected Not Detected10-061/ H23 Not Detected Not Detected Not Detected Not Detected10-061/ H24 Not Detected Not Detected Not Detected Not Detected10-061/ H25 Not Detected Not Detected Not Detected Not Detected10-061/ H26 Not Detected Not Detected Not Detected Not Detected10-061/ H27 Not Detected Not Detected Not Detected Not Detected10-061/ H28 Not Detected Not Detected Not Detected Not Detected10-061/ H29 Not Detected Not Detected Not Detected Not Detected10-061/ H30 Not Detected Not Detected Not Detected Not Detected


Veterinary Science/Microbiolo gyBuilding 90, Room 20IP.O. Box 210090Tucson, /Z8572I-0090

Tnr, UNvr.Rsrry on

AruzoNR.TucsoN AruzoNn

AQUACULTURE PATHOLOGYOFFICE PHONE \-520-621-8414

LAB PHONE 1-520-621-4438FAX 1-520-621-4899

rel: (520) 621-2355Fax: (520) 621-6366

.Teff ProchaskaPT llibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi173IOINDONESIA

E-mail : j eff.prochaska@) gmai l.com

Dear Mr. Prochaska:

September 30, 2010

Case: 10-282

The tests you requested for the detection of WSSV, IF{}{NV, MBV, HPV, BP, NFIP, IN{l'lV.YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Lifopenaeusvannamei from the PT BU BMC facility arrived on September 22"d,2010 in good condition. Allofthe samples were pooled in groups of 5 samples (15 to 34 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV, TSV. YFIV, IMNV, MBV. BP, HPV. NllPand IHI-NV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the lbllou'ing page.

We hope that this information will be helpful to you. A hard copy of this report r,vill bemailed to you. The fee for these tests is $ 1 ,224.00 (6 sets of tests for WSSV/IFIIIV @ $4 7.00 each.6 sets of samples for TSV/YHV @ $47.00 each, 6 sets of samples for IIVINV @521.00 each, 6 setsof samples for MBV/HPV @) $47.00, and 12 sets of tests for BP and NHP @ $21.00 each). Aseparate invoice will be sent to you. If there are any questions regarding this case please feel freeto contact us.

tlAZ Polic)' on certi fcation: This repof prov ides our fndings on the sanrples subnr itted to our laboratorl for eraminalion. heallh status er, al uation.disease diagnosis. or pathogen detection. It is our policir and intent to perfornr the ntost appropriate assa.v(s) for the deternrin:rtion ol rhc

a "certificate'' of health/pathogen status fbr thc sample(s) tested or lbr the sbcks. or thcilit,v. fiont uhich the sarnple(s) '"vcrc clerived.

laborator-v fbr the detection ofshrirnp pathogens is a research tool. lhe rcsults should bc oonsiclered as experimcnlal and tentative. Whencvcr

Carl R. PantoiaSolangell{avalroResearch Specialist

Page I of 3

As iate Research Professor

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Veterinary Science/MicrobiologyBuilding 90, Room 201P.O. Box 210090Tucson, 1Z85721-0090

Tnr UNvrRsrvor

AruzoNA.TucsoN AruzoNa

AQUACULTI]RE PATHOLOGYOFFICE PHONE t-52{t-62t-81t4

LAB PHONE 1-520- 621-4438FAX 1-520-621-4899

Tel: (520) 621-2355Fax: (520) 62L-6366

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA

E -mail : j eff.prochaska@gmail. corn

Dear Mr. Prochaska:

September 30, 2010

Case: 10-281

The tests you requested for the detection of WSSV, IHFNV, MBV. HPV, BP. NHP. IMNV,YHV & TSV by PCR have been completed. 'fhe 30 postlarvae and 60 pleopods from Lilopenaeusvannamei from the PT BU NBC facility anived on September 22"d,2010 in good condition. All ofthe samples were pooled in groups of 5 samples ( I 7 to 3 5 mg) per your instructions. fhe sampleswere collected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, FIPV, NHPand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this reporl will be

mailedtoyou. Thefeeforthesetestsis$1,224.00(6setsoftestsfbrWsSV/IHHV@$47.00each.6 sets of san,ples for TSV/YHV @ 947 .00 each, 6 sets of samples tbr IMNV (q, $21 .00 each. 6 sets

of samples for MBV/FIPV @ $47.00. and 12 sets of tests for BP and NHP @ $21.00 each). Aseparate invoice will be sent to you. If the.re are any queslions regarding this case please fbel free

to contact us.

UAZ Policy on certification: This report provides our findings on the sarnples submitted to our laboratory fur exarnination. health status evaluation.disease diagnosis, or pathogen detection. It is our policy and intent to perfbrm the most appropriate assay(s) for the deterrnination of thehealth/pathogen status ofall specimens submitted to our laboratory. Horvever. this report in no rva.v constitutes a stock or lacility "certification'' ora "certificate" ofhealth/pathogen status for the sample(s) tested or for the siocks, or facility. fiorn r.vhich the sarrrple(s) were derived.PCR: disclaimer: This report provides our findings on the samples submitted to our laboratory fbr pathogen detection. 'fhe PCR assal, used bl,' thislaboratory for the detection ofshrimp pathogens is a rescarch tool. '[he lesults should be considered as experime.ntal and tentativc. Whcneverpossible. PCR results should be confirmed by alternative assay. 1'his repofi in no rvay constitutes a stock or facilitl"'certificatron" or a "certiticate"of health/pathogen status lbr the sanrple(s) tested or fcrr the stocks. or tacilit_v-'. fiom lvhich rhe sampie(s) rvere derivcd.

Sincerely

Research Specialist Research Professor

Page I of 3

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Veterinary Science/MicrobiologyBuilding 90, Room 20IP.O. Box 210090Tucson, AZ8572l-0090

TnE UNrvr,nsrv or

AnrzoNn.Tucsox Aruzorue

AQUACULTURE PATHOLOGYoFFrcE PHONE t-520-621-8414

LAB PHONE 1-520-621-4438FAX 1-520-621-4899

Tel: (520) 621-2355Fax: (520) 621-6366

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310NDONESIA

E-mail : j [email protected]

Dear Mr. Prochaska:

\r,,-jiiU rj'* "&,! V. r-t ,1*1.*o'-*"tfv. Lightner Ph.D. )

Professor

March 29,20II

Case: 11-118

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV byPCRhave been completed. The 60 postlarvae and 120 pleopods from Litopenaeusvannamei from the PT BU BMC and PT BU NBC facilities arrived on March 25'h.2011 in goodcondition. All of the samples were pooled in groups of 5 samples (26 to 41 mg) per your instructions.The samples were collected for DNA and RNA extractions. WSSV, TSV, YHV,IN{NV, MBV, BP.HPV, NHP and IHHNV were Not Detected in any ofthe samples tested. A summary of the tests andresults is provided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $2,592.00 (12 sets oftests for WSSV/IHHV @ $50.00 each,

12 sets of samples for TSV/YHV @ $50.00 each,72 sets of samples for IMNV @$22.00 each, 12

sets of samples for MBV/HPV $50.00, and 24 sets of tests for BP and NHP @ $22.00 each). Aseparate invoice will be sent to you. If there are any questions regarding this case please feel freeto contact us.

UAZ Policy on certification: This report provides our findings on the samples submitted to our laboratory lor examination" health status evaluation,disease diagnosis. or pathogen detection. It is our policy and intent to perform the most appropriate assay(s) fbr the determination of thehealth/pathogen status ofall specimens submitted to our Iaboratory. However, this report in no way constitutes a stock or facility "cefiification" ora "certificate'" olhealth/pathogen status fbr the sample(s) tested or for the stocks. or facility, lrom which the sample(s) were derived.PCR: disclaimer: This report provides our tlndings on the samples submitted to our laboratory fbr pathogen detection. The PCR assay used b-v thislaboratoryforthedetectionofshrimppathogensisaresearchtool. Theresultsshouldbeconsideredasexperimental andlentative. Wheneverpossible, PCR results should be conilrmed by alternative assay. This report in no way constitutes a stock or facility "certification" or a ''certificate"ofhealth/pathogen status for the sarnple(s) tested or for the stocks, or facility, from which the sample(s) were derived.

Research Specialist

Page 1 of 4

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TnE UNrvr,nsrv or

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LAB PHONE 1-520-621-4438FAX 1-520-621-4899

Tel: (520) 621-2355Fax: (520) 621-6366

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310NDONESIA

E-mail : j [email protected]

Dear Mr. Prochaska:

\r,,-jiiU rj'* "&,! V. r-t ,1*1.*o'-*"tfv. Lightner Ph.D. )

Professor

March 29,20II

Case: 11-118

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV byPCRhave been completed. The 60 postlarvae and 120 pleopods from Litopenaeusvannamei from the PT BU BMC and PT BU NBC facilities arrived on March 25'h.2011 in goodcondition. All of the samples were pooled in groups of 5 samples (26 to 41 mg) per your instructions.The samples were collected for DNA and RNA extractions. WSSV, TSV, YHV,IN{NV, MBV, BP.HPV, NHP and IHHNV were Not Detected in any ofthe samples tested. A summary of the tests andresults is provided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $2,592.00 (12 sets oftests for WSSV/IHHV @ $50.00 each,

12 sets of samples for TSV/YHV @ $50.00 each,72 sets of samples for IMNV @$22.00 each, 12

sets of samples for MBV/HPV $50.00, and 24 sets of tests for BP and NHP @ $22.00 each). Aseparate invoice will be sent to you. If there are any questions regarding this case please feel freeto contact us.

UAZ Policy on certification: This report provides our findings on the samples submitted to our laboratory lor examination" health status evaluation,disease diagnosis. or pathogen detection. It is our policy and intent to perform the most appropriate assay(s) fbr the determination of thehealth/pathogen status ofall specimens submitted to our Iaboratory. However, this report in no way constitutes a stock or facility "cefiification" ora "certificate'" olhealth/pathogen status fbr the sample(s) tested or for the stocks. or facility, lrom which the sample(s) were derived.PCR: disclaimer: This report provides our tlndings on the samples submitted to our laboratory fbr pathogen detection. The PCR assay used b-v thislaboratoryforthedetectionofshrimppathogensisaresearchtool. Theresultsshouldbeconsideredasexperimental andlentative. Wheneverpossible, PCR results should be conilrmed by alternative assay. This report in no way constitutes a stock or facility "certification" or a ''certificate"ofhealth/pathogen status for the sarnple(s) tested or for the stocks, or facility, from which the sample(s) were derived.

Research Specialist

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Sincerely

Research Specialist

Tel: (520) 62I-2355Fax: (520) 621-6366Tu6oN Aruzoun


LAB PHONE 1-520-621-4438FAX 1-520-621-4899

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi173t0INDONESIA

E-mail : j eff.prochaska@ gmail. com

Dear Mr. Prochaska:

September 28,2011

Case: 11-334 NBC

#0 I 8/Bu-Lombok/1 409 I 1

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Litopenaeusvannamei from the PT BU NBC facilities arrived on Septemb er 26th,201 1 in good condition. Allof the samples were pooled in groups of 5 samples (10 to 30 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV, TSV, YHV,IMNV, MBV, BP, HPV, NHPand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $ 1,296.00 (6 sets of tests for WSSV/IHHV @ $50.00 each,6 sets of samples for TSV/YHY @ $50.00 each,6 sets of samples for IMNV @$22.00 each, 6 sets

of samples forMBV/HPV $50.00, and12 sets of tests forBP andNHP @$22.00 each). A separateinvoice will be sent to you. If there are any questions regarding this case please feel free to contactus.

UAZ Policy on certification: This report provides our findings on the samples submitted to our laboratofy for examination, health status evaluation,disease diagnosis, or pathogen detection. It is our policy and intent to perform the most appropriate assay(s) for the determination of thehealth/pathogen status ofall specimens submitted to our laboratory. However, this report in no way constitutes a stock or lacility "certification" ora "certificate" ofhealth./pathogen status for the saqple(s) tested or for the stocks, or facility, from which the sample(s) were derived.PCR: disclaimer: This report provides our findings cin the samples submitted to our laboratory for pathogen detection. The PCR assay used by thislaboratory for the detection of shrimp pathogens is a research tool. The results should be considered as experimental and tentative. Wheneverpossible, PCR results should be confirmed by alternative assay. This report in no way constitutes a stock or facility "certification" or a "certificate"ofhealth/pathogen status for the sample(s) tested or for the stocks, or facility, from which the sample(s) were derived.

,[\ r),[/*"t4v. V.l"h v.*.'-'D.V. Lightner Ph.D. JProfessor

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AQUACULTURE PATHOLOGYOFFICE PHONE | -520 -621 -841 4

LAB PHONE 1-520-621-4438FAX 1-520-621-4899

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA

E-mail : jeff. prochaska@gmai I.com

Dear Mr. Prochaska:

September 28,2011

Case: 11-335 BMC

#0 l8/BU-Lombok/1 4091 I

The tests you requested lor the detection of WSSV, IHHNV, MBV, HPV, BP, NHP, IMNV,YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Litopenaeusvannamei from thePT BU BMC facilities anived on September 26th,2011 in good condition. Allof the samples were pooled in groups of 5 samples (10 to 29 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV, TSV, YHV, IMNV, MBV, BP, HpV, NHpand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $1,296.00 (6 sets of tests for WSSV/IHHV @ $50.00 each,6 sets of samples for TSV/YHY @ $50.00 each, 6 sets of samples for IMNV @922.00 each, 6 setsofsamplesforMBV/HPVS50.00, and12 setsoftestsforBPandNHP @$22.00 each). Aseparateinvoice will be sent to you. If there are any questions regarding this case please feel free to contactus.

UAZ Policy on certification: This report provides our findings on the samples submitted to our laboratory for examination, health status evaluation,disease diagnosis, or pathogen detection. It is our policy and intent to perform the most appropriate assay(s) for the determination ol thehealth/pathogenstatusofallspecimenssubmittedtoourlaboratory. However,thisreportinnowayconstitutesastockorfacility"certification"ora "certificate" ofhealth/pathogen status for the sample{s) tested or for the stocks, or facility, from which the sample(s) were derived.PCR:disclaimer:Thisreportprovidesourfindingsbhthesamplessubmittedtoourlaboratoryforpathogendetection. ThePCRassayusedbythislaboratory for the detection of shrimp pathogens is a research tool. The results should be considered as experimental and tentative. Wheneverpossible, PCR results should be confirmed by alternative assay. This report in no way constitutes a stock or facility "certification" or a "certificate"ofhealth/pathogen status for the sample(s) tested or lor the stocks, or facility, from which the sample(s) were derived.

Sinceqely yg,urF. i' i )

Ms. Soldngel NaVarro D.V. Lightner Ph.D. "Research Specialist

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AQUACULTURE PATHOLOGYOFFICE PHONE t-520-621_8414

LAB PHONE 1-520- 621_4438FAX 1-520-621-4899

JeffProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA

E-mai I : j eff.prochaska@gmai l.com

Dear Mr. Prochaska:

Iune 6.2072

Case: 12-159 BMC

The tests you requested for the detection of WSSV, IHHNV, MBV, HpV, Bp, NHp, IMNV,YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Litopenaeusvannamei from thePT BU BMC facilities arrived on April 25th,2012 in good condition. All of thesamples were pooled in groups of 5 samples ( 1 3 to 26 mg) per your instructions. The samples werecollected for DNA and RNA extractions. WSSV, TSV, yHV, IMNV, MBV, Bp, Hpv, NHp andIHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $1,296.00 (6 sets of tests for WSSV/IHHV @ $50.00 each,6 sets of samples for TSV/YHY @ $50.00 each, 6 sets of samples for IMNV @$22.10 each, 6 setsof samples for MBV/HPV $50.00, and 72 sets of tests for BP and NHP @522.00 each). A separateinvoice will be sent to you. trf thete are any questions regarding this case please feel free to contactus.

UAZ Policy on certification: This report provides our findings olt the samples submitted to our laboratory for examination, health status evaluation,disease diagnosis, or pathogen detection. It is our policy and intent to perform the rnost appropriate assay(s) for the determination of thehealth/pathogen status ofall specimens submitted to our laboratory. However, this report in no way constitutes a stock or facility "certitication,'ora "oertificate" ofhealth/pathogen status for the samplels) tested or for the stocks, or facility, from which the sample(s) were derived.PCR:disclaimer:Thisreporlprovidesourfindings6nthesamplessubmittedtoourlaboratoryforpathogendetection. ThepCRassayusedbythislaboratoryforthedetectionofshrimppathogensisaresearchtool. Theresultsshouldbeconsideredasexperimentalandtentative. Wheneverpossible, PCR results should be confirmed by alternative assay. This report in no way constitutes a stock or faaility "certification" or a..certificate"of health/pathogen status for the sample(s) tested or lor the stocks, or facililfrom which the sample(s) were derived.

b;'rj;Y\i__-D.V. Lightner Ph.D.Professor

Sincerelv voAtJ

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AQUACULTURE, PATHOLOGYOFFICE PHONE t-520_621_8414

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JeffProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasitnlaINDONESIA

E-mail : jeff.prochaska@gmai l.com

Dear Mr. Prochaska:

June 6, 2015

Case: 12-158 NBC

The tests you requested for the detection of WSSV, IHHNV, MBV, HpV, Bp, NHp, IMNV,YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Litopenaeusvannamei from the PT BU NBC facilities arrived on April 25th,2012 in good condition. All of thesamples were pooled in groups of 5 samples (12 to 24 mg) per your instructions. The samples werecollected for DNA and RNA extractions. WSSV, TSV, yHV, IMNV, MBV, Bp, Hpv, NHp andIHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this report will bemailed to you. The fee for these tests is $ 1,296.00 (6 sets of tests for WSSV/IHHV @ $S0.00 each,6 sets of samples for TSV/YHV @ $50.00 each, 6 sets of samples for IMNV @$22.00 each, 6 setsof samples for MBV/HPV $50.00, and 12 sets of tests for BP and NHP @$ZZ.OO each). A separateinvoice will be sent to you. If there are any questions regarding this case please feel free to contactus.

UAZ Policy on certificafion: This report provides our findings on the samples submitted to our laboratory for examination, health status evaluation,disease diagnosis, or pathogen detection. [t is our policy and intent to perlorm the most appropriate assay(s) for the determination of thehealth/pathogen status ofall specimens submitted to our laboratory. However, this report in no way constitutes astock or facility..cerIification,'ora "certificate" olhealth/pathogen status for the samglels) tested or for the stocks, or facility, from which the sample(s) were derived.PCR:disclaimer:Thisreportprovidesourfindingsbnthesamplessubmittedtoourlaboratoryforpathogendeteciion. ThepCRassayusedbythislaboratory for the detection ofshrimp pathogens is a research tool. The results should be considered as experimental and tentative. Wheneverpossible, PCR results should be confirmed by alternative assay. This report in no way constitutes a stock or faiility "certification" or a..certificate,'ofhealth/pathogen status for the sample(s) tested or for the stocks, or facility-ftem which the sample(s) were deiived

Research Specialisl Professor

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Jelf ProchaskaPT Bibir UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310INDONESIA

E-mail : i efT. [email protected]

Dear Mr. Prochaska:

Veterinary Science and MicrobiologyBuildlng 90, Room 201PO. Box 210090Tucson, AZ 85221-0090Tel: (520) 6T.-2355Fax; (520) 62I-6366

Octoaber 29.2012

Case: 12-384 NBC

The tests you reqllested for trre detection of wSSV, IHHNV, MBV, Hpv, tsp, NHp, IMNV,YHV & TSV by PCR have been completed. The 30 postlarvae and 30 pleopods tiom Litopenaeus.vannamei from the PT BU NBC facilities arrived on o.tub.. 25'h,201'2-i;;; condition. All ofthe samples were pooled in groups ol5 samples ( I I to 3 5 mg) per your instrictions. The sampleswere collected fbr DNA and RNA extractions. wssv, r.si. vuv, IMNV, MBV, Bp, Hpv, NHpand IHHNV were Not Detected in any of the samples tested. A summary of the tests and results isprovided on the following page.we hope that this information *]l] !. helpful to you. A hard copy of this reporr will bemailed to you' The fee for these tests is $930.00 (i sets oii.rt, for wSSVIIHHy @ $50.00 each,3 sets of samples for TSV/yHV @ $50.00 each,3 sets of samples for IMNV (a$22.00each, 6 setsof samples for MBV/HPV $50.00, and l2sets oftests for Bp and NHp qszzlooeach). A separateinvoice will be sent to you. If there are any questionr..guroing;hi;;X;;"r#.t free to contactus.

discase diagnosis' or pathogen detectiorr lt is our pniily uno intent to perfbnn tlre'rnost appropriate assay(s) fbr the deterrnrnation 01.thea "cerfiflcale" ofhealth/pathogen status for the sample(s) tcsted or lbr the stocks. o, lo.iiity fiorn rvhich the sanrple(s) were rjerived.

o1'health/pathogen sttltus lbr tht' sanrplels; tested or t-or the stocks. or tacilitl. liom ivhich the sample(s) were derived.

Sincer-ely

R. Panto.ja

te Research Professor

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THE UNIVERSIryOF ARIZONA.

AQUACULTURE PATHOLOGYoFFlcE PHONE t-520-621-841 4

LAB PHONE 1 -520-621-4438FAX 1-520-621-4899

Jeff ProchaskaPT Bibit UnggulJL. Raya NarogongKm 14 Desa Cikiwul Bantar GebangBekasi17310

INDONESIA

E-mail : j eff.prochaskafd grnail.com

Dear Mr. Prochaska:

Veterinary Science and MicrobiologyBuilding 90, Room 201PO. Box 210090Tucson, AZ 8572f-0090Tel: (520) 621-2355Fax: (520) 621-6366

October 29,2012

Case: 12-385 BMC

The tests you requested for the detection of WSSV, IHHNV, MBV, HPV, BP, NHP. IMNV.YHV & TSV by PCR have been completed. The 30 postlarvae and 60 pleopods from Litopenaeusvannamei from thePT BU BMC facilities arrived on October 25'h,2012 in good condition. All ofthe samples were pooled in groups of 5 samples (19 to 50 mg) per your instructions. The sampleswere collected for DNA and RNA extractions. WSSV. TSV. YHV, IMNV, MBV. BP, HpV, NHpand IHHNV were Not Detected in an-v of the samples tested. A summary of the tests and results isprovided on the following page.

We hope that this information will be helpful to you. A hard copy of this reporr wiil bemailed to you. The fee for these tests is $ 1,296.00 (6 sets of tests fbr WSSV/IHHV @ $50.00 each,6 sets of samples for TSV/YHY @ $50.00 each, 6 sets of samples for IMNV @522.00 each, 6 setsof sanrples for MBV/HPV $50,00, and \2 sets of tests for BP and NHP @522.00 each). A separateinvoice will be sent to you. If there are any questions regarding this case please feel free to contactus.

a "certiflcate" olhealth/pathogen status 1br the sample(s) tested or fbr the stocks. or lacility. liont r,vhich the sanrple(s) rvere clerived.

laboratorl'fbr thc detectiorr olshrimp pathogcns is a research tool. The results should bc consicicreci as experimcntal a1d tentatiye. Whelevcr

ot health/pathogen status lbi the sanrple(s) tested or lbr the stocks. or lacilrty', fiorn lvhich the sanrplcis) were derivecl.

Sincerely

. SolangelT!aResearch Specialist

Carlos R. Pantoja

Page I of 3

Associate Research Professor

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AQUACULTURE PATHOLOGY OFFICE PHONE 1-520-621-8414 … · JL Kemang Amarilis 2 Blok AH No. 2 Perumanhan Kemang Pratama 2 Bekesi INDONESIA Case: 08-056 E-mail: [email protected]

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