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Figure 1B. 34 rice varieties with a sub-set of nine InDel markers
Figure 2. Example of ISSR fragments separated on the Bioanalyser. The problem of variable band intensity meant that this marker system was not considered further.
Pusasugandha
II
Pusasugandha
I
Supra
Pak 386
SherbatiA
SherbatiII
SherbatiI
ShaheenB
as
Basm
ati 2000
Super
PusaB
asmati 1
Taraori
Figure 3.. INDEL Multiplex panel 1 (IMPX1).
Figure 4a. Four INDEL variety test
Figure 4b. Four INDEL variety test
Figure 4a. Four INDEL variety test
Figure 5. Agilent fingerprint profiles for 12 different samples tested with 4-INDEL test. (Note ignore the band between 400 and 500 bp in lane 4; this is an artifact of the software).
Figure 6. Manual Height Threshold (FU) indicated by a green line is reduced from 20 to 2 which allows the area for the 168 non-basmati peak to be given.
Figure 7a. INDEL B8 Quantitative test showing replicate test samples
Figure 7. b. INDEL B8 quantitative test showing standards
Quantitative SOP Validation
0.07.0
14.021.028.035.042.049.056.063.070.077.084.0
CEQ Agilent Agilent Agilent Agilent
RM201 SOP1(RM201)
SOP1(RM201)
SOP2(INDEL)
SOP2(INDEL)
FDNAS FDNAS WSS FDNAS WSS
Estim
ated
% n
on-b
asm
ati
2861
3021
3028
3101
Figure 8 Validation of Quantitative SOPs in two laboratories (WSS and FDNAS). The results for four different commercial “unknown”samples are shown relative to Quantification with RM201 on the CEQ 8000 (CEQ). Note the permitted level of non-basmati is 7%.