ATM / ATR Perforin CTL IRE1α Caspase-10 Caspase-3 Caspase-6 Caspase-12 Caspase-9 ASK1 MKK7 PKC PI3K Akt PDK1 PIP2 PIP3 p90RSK Bad Bax Bim Bid tBid Bcl-2 Bcl-2 TRAF2 ASK1 IAP Apaf-1 Cytochrome c p53 p53AIP1 Bcl-x Ca 2+ ICAD CAD CAD DNA damage DNA fragmentation DNA Repair Cell shrinkage Membrane blebbing Growth Factors Cytokines Irradiation Fas ligand AIF Chromatin condensation Smac/DIABLO Smac/DIABLO Acinus PARP ER Stress Death Ligand- Death Receptor Caspase-8 pro-Caspase-8 pro-Caspase-3 pro-Caspase-3 pro-Caspase-3 DNA Fragmentation Cytochrome c Release from Mitochondria Caspase Activation Annexin V PI PI Fas DcR3 Daxx FADD DEDAF JNK Granzyme B Caspase-3 Cell Death Apoptosis Caspase-3 Withdrawal of Growth Factors Cytokines Apoptosis Inducer Phospholipid Translocation Annexin V Kits MEBCYTO ® Apoptosis Kit Annexin V Apoptosis Detection Kits FITC, EGFP, PE, Cy3, Cy5, Biotin Fas ELISA Kit Fas Ligand ELISA Kit TUNEL Kits MEBSTAIN TM Apoptosis Kit Direct MEBSTAIN TM Apoptosis Kit II DNA Ladder Detection Kits Quick Cell Proliferation Assay Kit Live/Dead Double Staining Kit Under development Cell Isolation Apoptotic Cell Isolation Kit Magnetic Separator Cell Fractionation Cytochrome c ELISA Kit Cytochrome c Releasing Apoptosis Assay Kit MitoCapture TM Apoptosis Detection Kit ApoSENSOR TM Cell Viability Assay Kit ApoSENSOR TM ADP/ATP Ratio Assay Kit AP1 PUMA Noxa JNK Granzyme B AIF Mitochondria/Cytosol Fractionation Kit Nuclear/Cytosol Fractionation Kit pro-Caspase-7 CaspGLOW TM Active Caspase Staining Kits Caspase Protease Assay Kits Colorimetric, Fluorometric CaspSCREEN TM Flow Cytometric Apoptosis Detection Kits CaspSELECT TM Immunoassay Kits International Corporation { Apoptosis Kits Apoptosis Signal Cascade and Detection Kits life.science.discovery. www.mblintl.com For Research Only Phagocytosis Apoptosis
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Apoptosis Apoptosis Kits Apoptosis Signal Cascade and … · 2019. 3. 21. · Flow Cytometry analysis of apoptosis by MEBCYTO® Apoptosis Kit in Jurkat cells treated with CH-11 for
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ATM /ATR
Perforin
CTL
IRE1α
Caspase-10
Caspase-3
Caspase-6
Caspase-12
Caspase-9
ASK1
MKK7
PKC
PI3K
Akt
PDK1
PIP2 PIP3
p90RSK
Bad
Bax
Bim
Bid
tBid
Bcl-2
Bcl-2
TRAF2
ASK1
IAP
Apaf-1
Cytochrome c
p53
p53AIP1Bcl-xCa2+
ICAD
CAD
CAD
DNA damage
DNA fragmentation
DNA Repair
Cell shrinkageMembrane blebbing
Growth FactorsCytokines
IrradiationFas ligand
AIF
Chromatin condensation
Smac/DIABLO
Smac/DIABLOAcinus
PARP
ER
Stress
Death Ligand- Death Receptor
Caspase-8
pro-Caspase-8
pro-Caspase-3
pro-Caspase-3pro-Caspase-3
DNA Fragmentation
Cytochrome c Release from Mitochondria
Caspase Activation
Annexin V
PI
PIFas
DcR3
Daxx FADD
DEDAF
JNK
Granzyme B
Caspase-3
Cell Death
ApoptosisCaspase-3
Withdrawal ofGrowth FactorsCytokines
Apoptosis Inducer
Phospholipid Translocation
Annexin V KitsMEBCYTO®Apoptosis KitAnnexin V Apoptosis Detection KitsFITC, EGFP, PE, Cy3, Cy5, Biotin
Fas ELISA KitFas Ligand ELISA Kit
TUNEL Kits
MEBSTAINTM Apoptosis Kit Direct MEBSTAINTM Apoptosis Kit II
DNA Ladder Detection Kits
Quick Cell Proliferation Assay Kit
Live/Dead Double Staining Kit
Under development
Cell Isolation
Apoptotic Cell Isolation KitMagnetic Separator
Cell Fractionation
Cytochrome c ELISA KitCytochrome c Releasing Apoptosis Assay Kit
This Kit utilizes MitoCaptureTM cationic dye, which has the unique property of being able to differentiate between normal
and apoptotic cells. In healthy cells, MitoCaptureTM accumulates and aggregates in the mitochondria, giving off a bright
red fluorescence. In apoptotic cells, MitoCaptureTM is prevented from aggregating in the mitochondria due to the altered
mitochondrial membrane potential.
MitoCaptureTM
Apoptosis Detection Kit
Reduction of red fluorescence intensity is shown in Jurkat cells after apoptosis induction by CH-11.
Open histogram: Untreated Jurkat cellsShaded histogram: Apoptosis induced Jurkat cells
Fluorescence intensity
Products
MitoCaptureTM Apoptosis Detection Kit
MitoCapture Incubation Buffer
Code No.
BV-K250-2
BV-K250-3
JM-1065-200
Quantity
25 tests
100 tests
200 mL
This kit contains unique formulations of reagents to isolate a
highly enriched mitochondrial fraction from cytosol. The procedure
is so simple and easy to perform, no ultracentrifugation is
required and no toxic chemicals are involved. Cytochrome c
is released from mitochondria into the cytosol. It can then be
detected by Western blotting using the cytochrome c antibody
provided with this kit.
Caspase dependency of cytochrome c release from mitochondria
HL60 cells were incubated with 5 μg/mL of Actinomycin D for 4hrs in the presence or absence of Z-VAD-FMK. DNA fragmentation was detected by TUNEL assay (A). Cytochrome c in the cytosol fraction was detected and measured by Western blot (B) and ELISA (C) respectively.
The Caspase (Colorimetric and Fluorometric) Protease Assay Kits detect
the cleavage of substrates peptide pNA or peptide-AFC in cell lysates. In
the colorimetric assay, the pNA can be quantified using a spectrophotometer
or microtiter plate reader at 405 nm. In the fluorometric assay, the substrate
peptide-AFC emits blue light (λmax=400 nm). Upon cleavage of the substrate
by caspases, the free AFC emits a yellow-green fluorescence (λmax =
505 nm) that can be quantified using a fluorometer or a fluorescence
microtiter plate reader. TUNEL-based detection KitsMeasurement of caspase activity in cell lysates from apoptosis induced Jurkat cells by CH-11 for indicated hours using Caspase Colorimetric Protease Assay Kits.
pN
A c
on
cen
tra
tion
(μM
)
Products
Caspase-1 Colorimetric Protease Assay Kit
Caspase-1 Fluorometric Protease Assay Kit
Caspase-2 Colorimetric Protease Assay Kit
Caspase-2 Fluorometric Protease Assay Kit
Caspase-3 Colorimetric Protease Assay Kit
Caspase-3 Fluorometric Protease Assay Kit
Caspase-4 Colorimetric Protease Assay Kit
Caspase-4 Fluorometric Protease Assay Kit
Caspase-5 Colorimetric Protease Assay Kit
Caspase-5 Fluorometric Protease Assay Kit
Code No.
BV-K111-2
BV-K111-3
BV-K111-13
BV-K111-4
BV-K110-2
BV-K110-3
BV-K110-13
BV-K110-4
BV-K117-2
BV-K117-3
BV-K117-13
BV-K117-4
BV-K116-2
BV-K116-3
BV-K116-13
BV-K116-4
BV-K106-2
BV-K106-13
BV-K106-4
BV-K105-2
BV-K105-13
BV-K105-4
JM-K127-25
JM-K127-100
JM-K126-25
JM-K126-100
BV-K123-2
BV-K123-3
BV-K123-13
BV-K123-4
BV-K122-2
BV-K122-3
BV-K122-13
BV-K122-4
Quantity
25 tests
100 tests
200 tests
400 tests
25 tests
100 tests
200 tests
400 tests
25 tests
100 tests
200 tests
400 tests
25 tests
100 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
200 tests
400 tests
25 tests
100 tests
200 tests
400 tests
Products
Caspase-6 Colorimetric Protease Assay Kit
Caspase-6 Fluorometric Protease Assay Kit
Caspase-8 Colorimetric Protease Assay Kit
Caspase-8 Fluorometric Protease Assay Kit
Caspase-9 Colorimetric Protease Assay Kit
Caspase-9 Fluorometric Protease Assay Kit
Caspase-10 Colorimetric Protease Assay Kit
Caspase-10 Fluorometric Protease Assay Kit
Caspase-12 Fluorometric Protease Assay Kit
Code No.
BV-K115-2
BV-K115-3
BV-K115-13
BV-K115-4
BV-K114-2
BV-K114-3
BV-K114-13
BV-K114-4
BV-K113-2
BV-K113-13
BV-K113-4
BV-K112-2
BV-K112-13
BV-K112-4
BV-K119-2
BV-K119-13
BV-K119-4
BV-K118-2
BV-K118-13
BV-K118-4
BV-K125-2
BV-K125-3
BV-K124-2
BV-K124-3
JM-K139-25
JM-K139-100
Quantity
25 tests
100 tests
200 tests
400 tests
25 tests
100 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
200 tests
400 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
Caspase Activity Detection Kits
For more information and to order go to www.mblintl.com6
APOPCYTOTM
Intracellular Caspase Activity Detection Kits
The APOPCYTO™ Intracellular Caspase Activity Detection Kits are designed to detect caspase activity in living cells using a non-toxic, cell permeable, fluorescent substrate. The kit contains all reagents necessary for the assay, including a control caspase inhibitor, washing buffer, and labeled substrate. Activated caspases can be detected easily and quickly in individual cells by fluorescence microscopy or flow cytometry (excitation 485 nm, emission 535 nm).
CaspGLOWTM Active Caspase Staining Kits provide a convenient and sensitive method for detecting in situ activated caspases in living cells. This assay utilizes a caspase family inhibitor conjugated to FITC (FITC-peptide-FMK) or sulfo-rhodamine (Red-peptide-FMK) as the fluorescent in situ marker. FITC-peptide-FMK and Red-peptide-FMK are cell permeable, nontoxic, and irreversibly bind to activated caspases in apoptotic cells. The fluorescent signal can be detected by fluorescence microscopy, flow cytometry, or a fluorescence plate reader. We have 9 different kits that vary by fluorochrome* and peptide sequence**.* FITC or sulfo-rhodamine** VDVAD or DEVD or IETD or LEHD or VAD
Products
CaspGLOWTM Fluorescein Active Caspase Staining Kit
CaspGLOWTM Red Active Caspase Staining Kit
CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit
CaspGLOWTM Fluorescein Active Caspase-3 Staining Kit
CaspGLOWTM Red Active Caspase-3 Staining Kit
CaspGLOWTM Fluorescein Active Caspase-8 Staining Kit
CaspGLOWTM Red Active Caspase-8 Staining Kit
CaspGLOWTM Fluorescein Active Caspase-9 Staining Kit
CaspGLOWTM Red Active Caspase-9 Staining Kit
Code No.
JM-K180-25
JM-K180-100
JM-K190-25
JM-K190-100
JM-K182-25
JM-K182-100
JM-K183-25
JM-K183-100
JM-K193-25
JM-K193-100
JM-K188-25
JM-K188-100
JM-K198-25
JM-K198-100
JM-K189-25
JM-K189-100
JM-K199-25
JM-K199-100
Quantity
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
25 tests
100 tests
CaspGLOWTM
(Fluorescein or Red) Active Caspase Staining Kits
Caspase Activity Detection Kits
Caspase Inhibitor Screening Kits
Inhibition of caspases can delay apoptosis, implicating a
potential role in drug screening efforts. The Caspase Inhibitor
Screening Kits provide an effective means for screening
caspase inhibitors using fluorometric methods. Each kit
contains active caspase enzyme and a synthetic AFC-
labeled peptide substrate. Active caspase cleaves the substrate
to release free AFC which is then quantified by fluorometry.
Compounds to be screened are added to the reaction and
the level of inhibition of caspase activity is determined by
comparing the fluorescence intensity of samples with and
without the inhibitors. The assay is simple, straightforward,
and can be performed directly in microtiter plates.
Products
Caspase-1 Inhibitor Screening Kit
Caspase-2 Inhibitor Screening Kit
Caspase-3 Inhibitor Screening Kit
Caspase-4 Inhibitor Screening Kit
Caspase-5 Inhibitor Screening Kit
Caspase-6 Inhibitor Screening Kit
Caspase-7 Inhibitor Screening Kit
Caspase-8 Inhibitor Screening Kit
Caspase-9 Inhibitor Screening Kit
Caspase-10 Inhibitor Screening Kit
Code No.
JM-K151-100
JM-K152-100
JM-K153-100
JM-K154-100
JM-K155-100
JM-K156-100
JM-K157-100
JM-K158-100
JM-K159-100
JM-K160-100
Quantity
100 tests
100 tests
100 tests
100 tests
100 tests
100 tests
100 tests
100 tests
100 tests
100 tests
DNA Ladder Detection Kits
Product
MEBCYTOTM Apoptosis Ladder Detection Kit
Apoptotic DNA Ladder Isolation Kit
Quick Apoptotic DNA Ladder Detection Kit
Enhanced Apoptotic DNA Ladder Detection Kit
Code No.
GS-J0103
JM-K170-50
BV-K120-6
JM-K130-50
Quantity
30 tests
50 tests
50 tests
50 tests
MBL carries several kits for the isolation and detection of DNA fragmentation in apoptotic cells and tissues, so scientists can
select the optimum kit for their research needs. The MEBCYTO™ Apoptosis Ladder Detection Kit provides an economical
method to rapidly and efficiently isolate DNA fragments from multiple samples. The Apoptotic DNA Ladder Isolation Kit
selectively isolates DNA ladders without interference of regular genomic DNA, which significantly improves detection sensitivity.
This kit can detect apoptotic DNA ladder from both tissues and cells that contain as little as 5% or less apoptotic cell
population. The Quick Apoptotic DNA Ladder Detection Kit provides a fast, easy way to isolate DNA fragments in a single
tube, without extraction steps or columns, in less than 90 minutes. Finally, the Enhanced Apoptotic DNA Ladder Kit utilizes a
unique dye that is 5-10 times more sensitive than ethidium bromide for the ultimate assay sensitivity.
MEBCYTOTM Apoptosis Ladder Detection KitApoptotic DNA Ladder Isolation KitQuick Apoptotic DNA Ladder Detection KitEnhanced Apoptotic DNA Ladder Detection Kit
Detection of DNA ladder in apoptotic HL60 cells induced by actinomycin D for indicated hours. DNA ladders are readily apparent after 2 hours. 'M' means marker.
hours0M 1 2 3 4 5 M
For more information and to order go to www.mblintl.com7
Code No.
5251
5255
BV-K501-3
BV-K301-10
BV-K301-2500
JM-K258-30
JM-1999-1
JM-K256-100
JM-K266-100
Quantity
96 wells
96 wells
100 tests
500 tests
2500 tests
30 tests
1 each
100 tests
100 tests
sFas ELISA Kit
sFas Ligand ELISA Kit
Live/Dead Double Staining Kit
Quick Cell Proliferation Assay Kit
Apoptotic Cell Isolation Kit
Magnetic Separator
Mitochondria/Cytosol Fractionation Kit
Nuclear/Cytosol Fractionation Kit
Products
TUNEL-Based Detection Kits
The TUNEL-based detection kits utilize terminal deoxynucleotidyl transferase (TdT) to induce incorporation of labeled
dUTP at the free 3'-OH ends of fragmented DNA. MBL provides several different choices for TUNEL assays to meet your
research needs. The ApoDIRECT kit labels DNA with fluorescein-dUTP for detection of DNA fragmention by both flow
cytometry and histochemistry. The MEBSTAIN™ assay kits use proprietary buffers for improved sensitivity and lower
backgrounds than traditional TUNEL protocols. The MEBSTAIN™ Direct kit includes fluorescein-dUTP to directly label
DNA for visualization of DNA fragments at the single cell level, while the MEBSTAIN Apoptosis Kit II labels cells with
biotin-dUTP followed by avidin-FITC. The Apo-BrdU In Situ DNA Fragmentation Kit labels DNA with BrdU followed by
detection with FITC-conjugated anti-BrdU antibody. A similar kit, the Apo-BrdU Red kit substitutes TRITC-labeled
antibody, to permit staining of GFP or Azami-Green-transfected cells. Finally, the ApoBrdU-IHC DNA Fragmentation Kit
uses BrdU incorporation, biotinylated anti-BrdU antibody, and HRP-conjugated streptavidin to permit colorimetric staining
of frozen or paraffin-embedded tissue samples.
Detection of apoptosis by MEBSTAIN Apoptosis Kit Direct (left) and Apo-BrdU In situ DNA Fragmentation Assay Kit (right) in Jurkat cells treated with CH-11 for 7 hrs.
Open histograms: Untreated Jurkat cellsShaded histograms: Jurkat cells treated with CH-11
Detection of apoptosis by MEBSTAIN Apoptosis Kits in mouse ovary
A: MEBSTAIN Apoptosis Kit DirectB: MEBSTAIN Apoptosis Kit II
Fluorescence intensity
A B
Products
MEBSTAINTM Apoptosis Kit Direct
MEBSTAINTM Apoptosis Kit II
Apo-BrdU In Situ DNA Fragmentation Assay Kit
Apo-BrdU Red DNA Fragmentation Assay Kit
ApoDIRECT In Situ DNA Fragmentation Assay Kit
ApoBrdU-IHC DNA Fragmentation Kit
Code No.
8445
8441
JM-K401-60
JM-K404-60
JM-K402-50
JM-K403-50
Quantity
FCM: 66 tests / IHC: 40 tests
FCM: 66 tests / IHC: 40 tests
60 assays
60 assays
50 assays
50 assays
Additional Apoptosis-Related
Code No.
JM-K267-50
JM-K268-50
JM-K269-500
JM-K270-50
JM-K280-50
JM-K281-50
JM-K251-100
JM-K4360-1000
Quantity
50 tests
50 tests
500 tests
50 tests
50 tests
50 tests
100 tests
1000 tests
Cytosol/Particulate Rapid Separation Kit
Membrane Protein Extraction Kit
Mammalian Cell Extraction Kit
FractionPREP Cell Fractionation Kit
Mitochondrial DNA Isolation Kit
Genomic DNA Isolation Kit
ApoGSHTM Glutathione Detection Kit
Human TWEAK ELISA Development Kit
Products
International Corporation
International Corporation
International Corporation
International Corporation
International Corporation Medical & Biological Laboratories Co., LTD.4 H Constitution Way Tel: (781) 939-6964 KDX Nagoya Sakae Bldg. 10FWoburn, MA 01801, USA (800) 200-5459 4-5-3 Sakae, Naka-ku, Nagoya, Aichi Fax: (781) 939-6963 460-0008, JAPAN Tel: 81-52-971-2081 Fax: 81-52-971-2337URL: http://www.mblintl.com email: [email protected] URL: https://ruo.mbl.co.jp