Antioxidant and Hepatoprotective Properities of Nigella sativa L. seeds Extract Against APAP-Induced Liver stress In vivo and In vitro

ABSTRACTGareeballah Osman AdamGraduate School of Woosuk UniversityThe objective of this study was to investigate the antioxidant and hepatoprotective effects of Nigella sativa (NS) seeds on acetaminophen (APAP)-induced acute hepatotoxicity in male Sprague-Dawley rats. There were five experimental groups, including normal control (vehicle, n=6), APAP as a hepatotoxic control (APAP 800 B.w. I.P., n=6), NS I (APAP+NS 100 mg/kg b.w. P.O., n=6), NS II (APAP+NS 300 mg/kg b.w. P.O., n=6) and NS III (APAP+NS 900 mg/kg B.w. P.O., n=6). After 24 hours, enzymes such as superoxide dismutase (SOD) and glutathion (GSH) were significantly increased, whereas malondialdehyde (MDA) was significantly decreased (P