Antinociceptive and anti-inflammatory effects of the roots ethanolic

CADI AYYAD UNIVERSITYFACULTY OF SCIENCES SEMLALIA MARRAKECH

U.F.R: BIOLOGY AND HEALTHLABORATORY OF PHARMACOLOGY, NEUROBIOLOGY

AND BEHAVIOR

Antinociceptive and anti-inflammatory effects of the roots ethanolic extract of

Rubia peregrina

21st IBRO-ISN School of Neuroscience in Africa, Fayoum, Egypt

Supervisor:

Pr. A. CHAIT

Realised by:

Samira CHAIB

OUTLINE

Introduction

Material & methodes

Conclusion

Results & discussion

• 75% of african population still rely on traditional healing practices and medicinal plants for their daily healthcare needs.

•Morocco have an ancient and rich tradition in the field of herbal medicine, which makes the use of medicinal plants in treating diseases, an integrated part of Moroccan culture.

•One of such frequently Moroccan medicinal plants used is Rubia peregrina known as « FOUA ».

1.1 Introduction

1. Introduction 2. Materials and methode 3. Results and discussion 4. Conclusion

1. Introduction 2. Materials and methodes 3. Results and discussion 4. Conclusion and perspectives

1.2 systematic

Phylum: Angiosperms

Subphylum: Spermatophytes

Classe: Broadleaf

Order: Rubiales

Family: Rubiaceae

Gender: Rubia

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

Rubia peregrina

Liver disease Hypotension

Anemia Inflammation

1.3 Traditional use

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

1. Introduction 2. Materials and methodes 3. Results and discussion 4. Conclusion and perspectives

1.4 aims of study

Evaluation of the anti-nociceptive and anti-inflammatory effects of the root ethanolic extract of Rubia peregrina

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

Entrée d’eau

Sortie d’eauRéfrigérant

Matière à extraire

2.1 extraction methode

extraction dry continuous soxhlet

weight : 25-35g

LD photoperiodWater and food is provided Ad libitum

mals and femelles swiss albinos mice

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

2.2 biological materials

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

2.4 analgesic test (writhing test)

Evaluation of the sensitivity to nociceptive stimuli against chemical nociception

Writhing test

Fontenele, J.B., Viana, G.S., Xavier-Filho, J., de-Alencar, J.W., 1996. Anti-inflammatory and analgesic activity of a water-soluble fraction from shark cartilage. Brazilian Journal of Medical and Biological Research 29, 643–646

The nociceptive response is represented by the number of writhing performed by the animal tested

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

2.5 inflammatory test:

Chen, Q., 1993. Pharmacological Research Methods in Traditional Chinese Medicine. People’s Medical Publishing House, Beijing, p. 305.

treatment of mice with distilled water,

Diclofinac, or extract

applying a drop of xylene on both surfaces of the right ear

the animals were killed by cervical dislocation

both ears cut off, sized and weighed

Two hours

One hour

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

3.2 analgesic test (writhing test)

Controle

-

ASA25

0 mg/kg

500 m

g/kg

750 m

g/kg

Nb of writhing

Controle

-

Diclofin

ac

250 m

g/kg

500 mg/kg

750 mg/kg

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

3.3 inflammatory test:

1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion

The results of the study demostrate that the administration of the ethanolic extract from R.P roots exhibits anti inflammatory properties. There was a significant and dose depondant inhibition of the acuteoedema, And a significant inhibition of pain in the writhing test.